Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25476.t1 | XP_003628361.1 | 93.2 | 472 | 30 | 1 | 1 | 470 | 15 | 486 | 8.40E-243 | 849.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25476.t1 | Q9SWI1 | 36.2 | 473 | 179 | 22 | 12 | 443 | 21 | 411 | 8.4e-42 | 172.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25476.t1 | G7LA55 | 93.2 | 472 | 30 | 1 | 1 | 470 | 15 | 486 | 6.1e-243 | 849.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene25476 | 0.826579 | 2.51E-54 | -1.69E-46 |
| MS.gene049171 | MS.gene25476 | 0.846266 | 2.42E-59 | -1.69E-46 |
| MS.gene049173 | MS.gene25476 | 0.833548 | 5.01E-56 | -1.69E-46 |
| MS.gene049217 | MS.gene25476 | 0.818676 | 1.73E-52 | -1.69E-46 |
| MS.gene049222 | MS.gene25476 | 0.851121 | 1.09E-60 | -1.69E-46 |
| MS.gene049232 | MS.gene25476 | 0.839201 | 1.83E-57 | -1.69E-46 |
| MS.gene049285 | MS.gene25476 | 0.848457 | 6.06E-60 | -1.69E-46 |
| MS.gene049320 | MS.gene25476 | 0.847945 | 8.38E-60 | -1.69E-46 |
| MS.gene049396 | MS.gene25476 | 0.81823 | 2.18E-52 | -1.69E-46 |
| MS.gene049552 | MS.gene25476 | 0.807293 | 5.43E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene25476 | 0.861948 | 7.16E-64 | -1.69E-46 |
| MS.gene049633 | MS.gene25476 | 0.821567 | 3.77E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene25476 | 0.851881 | 6.65E-61 | -1.69E-46 |
| MS.gene05010 | MS.gene25476 | 0.8256 | 4.29E-54 | -1.69E-46 |
| MS.gene050163 | MS.gene25476 | 0.811236 | 7.75E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene25476 | 0.831753 | 1.40E-55 | -1.69E-46 |
| MS.gene050323 | MS.gene25476 | 0.835049 | 2.10E-56 | -1.69E-46 |
| MS.gene050333 | MS.gene25476 | 0.836788 | 7.62E-57 | -1.69E-46 |
| MS.gene050496 | MS.gene25476 | 0.804168 | 2.46E-49 | -1.69E-46 |
| MS.gene050502 | MS.gene25476 | 0.801172 | 1.02E-48 | -1.69E-46 |
| MS.gene050504 | MS.gene25476 | 0.832425 | 9.52E-56 | -1.69E-46 |
| MS.gene050518 | MS.gene25476 | 0.820944 | 5.25E-53 | -1.69E-46 |
| MS.gene050577 | MS.gene25476 | 0.853467 | 2.34E-61 | -1.69E-46 |
| MS.gene050593 | MS.gene25476 | 0.809664 | 1.69E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene25476 | 0.82505 | 5.79E-54 | -1.69E-46 |
| MS.gene050662 | MS.gene25476 | 0.824667 | 7.13E-54 | -1.69E-46 |
| MS.gene050675 | MS.gene25476 | 0.827151 | 1.83E-54 | -1.69E-46 |
| MS.gene050686 | MS.gene25476 | 0.814456 | 1.53E-51 | -1.69E-46 |
| MS.gene050699 | MS.gene25476 | 0.840627 | 7.76E-58 | -1.69E-46 |
| MS.gene050747 | MS.gene25476 | 0.860427 | 2.08E-63 | -1.69E-46 |
| MS.gene050772 | MS.gene25476 | 0.884099 | 2.56E-71 | -1.69E-46 |
| MS.gene05080 | MS.gene25476 | 0.830631 | 2.63E-55 | -1.69E-46 |
| MS.gene050868 | MS.gene25476 | 0.820721 | 5.91E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene25476 | 0.821814 | 3.31E-53 | -1.69E-46 |
| MS.gene050904 | MS.gene25476 | 0.809009 | 2.34E-50 | -1.69E-46 |
| MS.gene050950 | MS.gene25476 | 0.845003 | 5.32E-59 | -1.69E-46 |
| MS.gene050986 | MS.gene25476 | 0.820974 | 5.17E-53 | -1.69E-46 |
| MS.gene05100 | MS.gene25476 | 0.820608 | 6.27E-53 | -1.69E-46 |
| MS.gene051167 | MS.gene25476 | -0.800083 | 1.70E-48 | -1.69E-46 |
| MS.gene051387 | MS.gene25476 | 0.824675 | 7.10E-54 | -1.69E-46 |
| MS.gene051509 | MS.gene25476 | 0.812026 | 5.22E-51 | -1.69E-46 |
| MS.gene051551 | MS.gene25476 | 0.82543 | 4.71E-54 | -1.69E-46 |
| MS.gene051666 | MS.gene25476 | 0.818279 | 2.13E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene25476 | 0.826595 | 2.49E-54 | -1.69E-46 |
| MS.gene051858 | MS.gene25476 | 0.800908 | 1.16E-48 | -1.69E-46 |
| MS.gene051941 | MS.gene25476 | 0.831965 | 1.24E-55 | -1.69E-46 |
| MS.gene052164 | MS.gene25476 | 0.853891 | 1.77E-61 | -1.69E-46 |
| MS.gene052271 | MS.gene25476 | 0.819242 | 1.29E-52 | -1.69E-46 |
| MS.gene052318 | MS.gene25476 | 0.802382 | 5.76E-49 | -1.69E-46 |
| MS.gene052421 | MS.gene25476 | 0.831196 | 1.91E-55 | -1.69E-46 |
| MS.gene052482 | MS.gene25476 | 0.847481 | 1.13E-59 | -1.69E-46 |
| MS.gene052486 | MS.gene25476 | 0.85219 | 5.43E-61 | -1.69E-46 |
| MS.gene052501 | MS.gene25476 | 0.811411 | 7.09E-51 | -1.69E-46 |
| MS.gene052503 | MS.gene25476 | 0.854271 | 1.38E-61 | -1.69E-46 |
| MS.gene052504 | MS.gene25476 | 0.82597 | 3.51E-54 | -1.69E-46 |
| MS.gene052506 | MS.gene25476 | 0.848156 | 7.34E-60 | -1.69E-46 |
| MS.gene052615 | MS.gene25476 | 0.843113 | 1.71E-58 | -1.69E-46 |
| MS.gene052820 | MS.gene25476 | 0.815827 | 7.57E-52 | -1.69E-46 |
| MS.gene052822 | MS.gene25476 | 0.850982 | 1.19E-60 | -1.69E-46 |
| MS.gene052823 | MS.gene25476 | 0.850389 | 1.75E-60 | -1.69E-46 |
| MS.gene052825 | MS.gene25476 | 0.838643 | 2.55E-57 | -1.69E-46 |
| MS.gene052916 | MS.gene25476 | 0.830888 | 2.28E-55 | -1.69E-46 |
| MS.gene052966 | MS.gene25476 | 0.819896 | 9.13E-53 | -1.69E-46 |
| MS.gene053117 | MS.gene25476 | 0.815317 | 9.83E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene25476 | 0.829286 | 5.60E-55 | -1.69E-46 |
| MS.gene053207 | MS.gene25476 | 0.823489 | 1.35E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene25476 | 0.861996 | 6.93E-64 | -1.69E-46 |
| MS.gene053286 | MS.gene25476 | 0.860298 | 2.28E-63 | -1.69E-46 |
| MS.gene053436 | MS.gene25476 | 0.881997 | 1.51E-70 | -1.69E-46 |
| MS.gene053479 | MS.gene25476 | 0.80715 | 5.82E-50 | -1.69E-46 |
| MS.gene053480 | MS.gene25476 | 0.831155 | 1.96E-55 | -1.69E-46 |
| MS.gene053505 | MS.gene25476 | 0.812851 | 3.44E-51 | -1.69E-46 |
| MS.gene053574 | MS.gene25476 | 0.866926 | 1.99E-65 | -1.69E-46 |
| MS.gene053626 | MS.gene25476 | 0.864398 | 1.25E-64 | -1.69E-46 |
| MS.gene053632 | MS.gene25476 | 0.850821 | 1.33E-60 | -1.69E-46 |
| MS.gene053813 | MS.gene25476 | 0.801665 | 8.09E-49 | -1.69E-46 |
| MS.gene053824 | MS.gene25476 | 0.842111 | 3.16E-58 | -1.69E-46 |
| MS.gene053912 | MS.gene25476 | 0.817402 | 3.36E-52 | -1.69E-46 |
| MS.gene053916 | MS.gene25476 | 0.846221 | 2.49E-59 | -1.69E-46 |
| MS.gene054003 | MS.gene25476 | 0.801804 | 7.58E-49 | -1.69E-46 |
| MS.gene054408 | MS.gene25476 | 0.833985 | 3.89E-56 | -1.69E-46 |
| MS.gene054595 | MS.gene25476 | 0.854877 | 9.18E-62 | -1.69E-46 |
| MS.gene054600 | MS.gene25476 | 0.836971 | 6.84E-57 | -1.69E-46 |
| MS.gene054642 | MS.gene25476 | 0.845784 | 3.27E-59 | -1.69E-46 |
| MS.gene05470 | MS.gene25476 | 0.80482 | 1.80E-49 | -1.69E-46 |
| MS.gene054927 | MS.gene25476 | 0.830557 | 2.75E-55 | -1.69E-46 |
| MS.gene054954 | MS.gene25476 | 0.843479 | 1.37E-58 | -1.69E-46 |
| MS.gene05505 | MS.gene25476 | 0.856185 | 3.82E-62 | -1.69E-46 |
| MS.gene055206 | MS.gene25476 | 0.837419 | 5.26E-57 | -1.69E-46 |
| MS.gene055224 | MS.gene25476 | 0.807935 | 3.96E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene25476 | 0.815226 | 1.03E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene25476 | 0.856983 | 2.22E-62 | -1.69E-46 |
| MS.gene055502 | MS.gene25476 | 0.842427 | 2.60E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene25476 | 0.823705 | 1.20E-53 | -1.69E-46 |
| MS.gene055722 | MS.gene25476 | 0.854311 | 1.34E-61 | -1.69E-46 |
| MS.gene055748 | MS.gene25476 | 0.807065 | 6.06E-50 | -1.69E-46 |
| MS.gene055750 | MS.gene25476 | 0.828918 | 6.88E-55 | -1.69E-46 |
| MS.gene055751 | MS.gene25476 | 0.805244 | 1.47E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene25476 | 0.830433 | 2.95E-55 | -1.69E-46 |
| MS.gene055909 | MS.gene25476 | 0.836338 | 9.92E-57 | -1.69E-46 |
| MS.gene055911 | MS.gene25476 | 0.829404 | 5.25E-55 | -1.69E-46 |
| MS.gene05592 | MS.gene25476 | 0.800544 | 1.37E-48 | -1.69E-46 |
| MS.gene055941 | MS.gene25476 | 0.836613 | 8.44E-57 | -1.69E-46 |
| MS.gene055954 | MS.gene25476 | 0.811533 | 6.68E-51 | -1.69E-46 |
| MS.gene056040 | MS.gene25476 | 0.805365 | 1.38E-49 | -1.69E-46 |
| MS.gene056046 | MS.gene25476 | 0.811805 | 5.83E-51 | -1.69E-46 |
| MS.gene056166 | MS.gene25476 | 0.833177 | 6.20E-56 | -1.69E-46 |
| MS.gene056374 | MS.gene25476 | 0.833005 | 6.84E-56 | -1.69E-46 |
| MS.gene056409 | MS.gene25476 | 0.800742 | 1.25E-48 | -1.69E-46 |
| MS.gene056446 | MS.gene25476 | 0.828808 | 7.32E-55 | -1.69E-46 |
| MS.gene056448 | MS.gene25476 | 0.812593 | 3.92E-51 | -1.69E-46 |
| MS.gene056449 | MS.gene25476 | 0.818991 | 1.47E-52 | -1.69E-46 |
| MS.gene056450 | MS.gene25476 | 0.803941 | 2.74E-49 | -1.69E-46 |
| MS.gene056487 | MS.gene25476 | 0.826591 | 2.49E-54 | -1.69E-46 |
| MS.gene056504 | MS.gene25476 | 0.862075 | 6.55E-64 | -1.69E-46 |
| MS.gene056510 | MS.gene25476 | 0.842277 | 2.85E-58 | -1.69E-46 |
| MS.gene056511 | MS.gene25476 | 0.821527 | 3.85E-53 | -1.69E-46 |
| MS.gene056610 | MS.gene25476 | 0.802348 | 5.85E-49 | -1.69E-46 |
| MS.gene05664 | MS.gene25476 | 0.813972 | 1.95E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene25476 | 0.852849 | 3.52E-61 | -1.69E-46 |
| MS.gene056692 | MS.gene25476 | 0.822405 | 2.41E-53 | -1.69E-46 |
| MS.gene056878 | MS.gene25476 | 0.82186 | 3.23E-53 | -1.69E-46 |
| MS.gene057019 | MS.gene25476 | 0.806652 | 7.41E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene25476 | 0.837046 | 6.55E-57 | -1.69E-46 |
| MS.gene057086 | MS.gene25476 | 0.818782 | 1.64E-52 | -1.69E-46 |
| MS.gene057331 | MS.gene25476 | 0.804126 | 2.51E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene25476 | 0.854177 | 1.46E-61 | -1.69E-46 |
| MS.gene057658 | MS.gene25476 | 0.806369 | 8.51E-50 | -1.69E-46 |
| MS.gene057734 | MS.gene25476 | 0.851362 | 9.32E-61 | -1.69E-46 |
| MS.gene057831 | MS.gene25476 | 0.836383 | 9.66E-57 | -1.69E-46 |
| MS.gene057856 | MS.gene25476 | 0.822114 | 2.82E-53 | -1.69E-46 |
| MS.gene057857 | MS.gene25476 | 0.801944 | 7.09E-49 | -1.69E-46 |
| MS.gene05787 | MS.gene25476 | 0.845082 | 5.07E-59 | -1.69E-46 |
| MS.gene058019 | MS.gene25476 | 0.826157 | 3.16E-54 | -1.69E-46 |
| MS.gene058130 | MS.gene25476 | 0.802541 | 5.34E-49 | -1.69E-46 |
| MS.gene058157 | MS.gene25476 | 0.807486 | 4.94E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene25476 | 0.832353 | 9.92E-56 | -1.69E-46 |
| MS.gene05837 | MS.gene25476 | 0.829911 | 3.95E-55 | -1.69E-46 |
| MS.gene058419 | MS.gene25476 | 0.864743 | 9.76E-65 | -1.69E-46 |
| MS.gene058544 | MS.gene25476 | 0.835255 | 1.87E-56 | -1.69E-46 |
| MS.gene058773 | MS.gene25476 | 0.831477 | 1.63E-55 | -1.69E-46 |
| MS.gene058838 | MS.gene25476 | 0.847556 | 1.07E-59 | -1.69E-46 |
| MS.gene059022 | MS.gene25476 | 0.840674 | 7.54E-58 | -1.69E-46 |
| MS.gene059023 | MS.gene25476 | 0.840309 | 9.40E-58 | -1.69E-46 |
| MS.gene05913 | MS.gene25476 | 0.819185 | 1.33E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene25476 | 0.802727 | 4.89E-49 | -1.69E-46 |
| MS.gene059367 | MS.gene25476 | 0.836983 | 6.79E-57 | -1.69E-46 |
| MS.gene059368 | MS.gene25476 | 0.819597 | 1.07E-52 | -1.69E-46 |
| MS.gene059455 | MS.gene25476 | 0.844201 | 8.76E-59 | -1.69E-46 |
| MS.gene059667 | MS.gene25476 | 0.831988 | 1.22E-55 | -1.69E-46 |
| MS.gene059677 | MS.gene25476 | 0.832148 | 1.11E-55 | -1.69E-46 |
| MS.gene059711 | MS.gene25476 | 0.81272 | 3.68E-51 | -1.69E-46 |
| MS.gene059718 | MS.gene25476 | 0.84946 | 3.19E-60 | -1.69E-46 |
| MS.gene059756 | MS.gene25476 | 0.816174 | 6.34E-52 | -1.69E-46 |
| MS.gene059801 | MS.gene25476 | 0.848152 | 7.35E-60 | -1.69E-46 |
| MS.gene059865 | MS.gene25476 | 0.827611 | 1.42E-54 | -1.69E-46 |
| MS.gene06000 | MS.gene25476 | 0.817828 | 2.69E-52 | -1.69E-46 |
| MS.gene060482 | MS.gene25476 | 0.830798 | 2.40E-55 | -1.69E-46 |
| MS.gene060762 | MS.gene25476 | 0.835649 | 1.48E-56 | -1.69E-46 |
| MS.gene060778 | MS.gene25476 | 0.852095 | 5.78E-61 | -1.69E-46 |
| MS.gene06077 | MS.gene25476 | 0.852803 | 3.63E-61 | -1.69E-46 |
| MS.gene060835 | MS.gene25476 | 0.830214 | 3.33E-55 | -1.69E-46 |
| MS.gene060837 | MS.gene25476 | 0.824914 | 6.24E-54 | -1.69E-46 |
| MS.gene06092 | MS.gene25476 | 0.825714 | 4.03E-54 | -1.69E-46 |
| MS.gene06097 | MS.gene25476 | 0.847071 | 1.46E-59 | -1.69E-46 |
| MS.gene060982 | MS.gene25476 | 0.83566 | 1.47E-56 | -1.69E-46 |
| MS.gene061150 | MS.gene25476 | 0.830177 | 3.40E-55 | -1.69E-46 |
| MS.gene061171 | MS.gene25476 | 0.817495 | 3.20E-52 | -1.69E-46 |
| MS.gene061200 | MS.gene25476 | 0.800251 | 1.57E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25476.t1 | MTR_8g055860 | 96.610 | 472 | 14 | 1 | 1 | 470 | 15 | 486 | 0.0 | 942 |
| MS.gene25476.t1 | MTR_5g040340 | 47.327 | 505 | 178 | 22 | 3 | 470 | 7 | 460 | 1.41e-108 | 330 |
| MS.gene25476.t1 | MTR_7g088200 | 28.358 | 335 | 194 | 11 | 98 | 407 | 129 | 442 | 1.16e-14 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25476.t1 | AT2G02950 | 37.360 | 447 | 160 | 21 | 34 | 443 | 48 | 411 | 2.16e-50 | 178 |
| MS.gene25476.t1 | AT1G14280 | 33.182 | 440 | 142 | 22 | 61 | 444 | 74 | 417 | 7.36e-36 | 138 |
Find 84 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAAAGCTGTTAGAGTTTC+TGG | 0.087161 | 8.3:-43004685 | MS.gene25476:CDS |
| GCAGAAGAAATTGGAGTATT+TGG | 0.123215 | 8.3:-43005714 | MS.gene25476:CDS |
| GGTTTCAATGGTGTCCTTAT+TGG | 0.175910 | 8.3:+43004766 | None:intergenic |
| AGAAAATCACTTGAAGTATT+TGG | 0.180016 | 8.3:-43005126 | MS.gene25476:CDS |
| AATAACAAAACTTCTAAGTA+TGG | 0.262272 | 8.3:-43005381 | MS.gene25476:CDS |
| GGAGTTTCTATGGTTGATAA+AGG | 0.283830 | 8.3:+43005758 | None:intergenic |
| GATGAAGAGGGGAGTTTCTA+TGG | 0.288069 | 8.3:+43005748 | None:intergenic |
| CCTTCTTTCATATTCAAGTA+TGG | 0.298687 | 8.3:+43005825 | None:intergenic |
| TTTCCAGGAGAGACAAATTA+TGG | 0.316379 | 8.3:-43004574 | MS.gene25476:CDS |
| CACTCTTTAAGAGTGCATTT+TGG | 0.316916 | 8.3:+43005538 | None:intergenic |
| TTCAAAAGCAAGAGAAGTTA+AGG | 0.319574 | 8.3:-43005242 | MS.gene25476:CDS |
| TCTCAAGTGGCAAGGTTTCC+AGG | 0.321527 | 8.3:-43004589 | MS.gene25476:CDS |
| AAGCAATGAAAAGAATAGTT+TGG | 0.330151 | 8.3:-43005211 | MS.gene25476:CDS |
| TCACTCGGCGCGTAACAATT+CGG | 0.340359 | 8.3:+43004877 | None:intergenic |
| GTGGAAAAGGCAGAAGAAAT+TGG | 0.345679 | 8.3:-43005723 | MS.gene25476:CDS |
| TGATAAAGGGATTGATTGTT+TGG | 0.345694 | 8.3:+43005772 | None:intergenic |
| AGAAAGTACCAAGTTGAGTA+TGG | 0.364459 | 8.3:-43005597 | MS.gene25476:CDS |
| CACTCGGCGCGTAACAATTC+GGG | 0.365890 | 8.3:+43004878 | None:intergenic |
| TTATTGATCAATATTGGATT+TGG | 0.374889 | 8.3:+43005099 | None:intergenic |
| AGTGGAGTGAGAAATTCTAT+AGG | 0.378295 | 8.3:-43005519 | MS.gene25476:CDS |
| TGAAGCATGTGGCGAGTTAG+AGG | 0.378519 | 8.3:+43004501 | None:intergenic |
| GGTGTCTCTTTAGCGACCTT+TGG | 0.382312 | 8.3:+43004745 | None:intergenic |
| CGGCGGCCTGGCATGTTGTT+GGG | 0.387012 | 8.3:-43004718 | MS.gene25476:CDS |
| AGACAAATTATGGTTCAAAA+AGG | 0.388140 | 8.3:-43004564 | MS.gene25476:CDS |
| GCGGCGGCCTGGCATGTTGT+TGG | 0.388150 | 8.3:-43004719 | MS.gene25476:CDS |
| TTAGCGACCTTTGGTTTCAA+TGG | 0.392681 | 8.3:+43004754 | None:intergenic |
| GAAATTGATAATGCAAACTA+TGG | 0.394797 | 8.3:-43005024 | MS.gene25476:CDS |
| TTACAATAAGGAGTCCAATA+AGG | 0.397883 | 8.3:-43004780 | MS.gene25476:CDS |
| ATGAATTAGTATAGTGCAAA+AGG | 0.407405 | 8.3:-43004270 | MS.gene25476:intron |
| TCTAAGTATGGTGCAGTTCA+AGG | 0.411902 | 8.3:-43005369 | MS.gene25476:CDS |
| GAACCATAATTTGTCTCTCC+TGG | 0.414611 | 8.3:+43004571 | None:intergenic |
| CCCTCTTCATCAAGTGGAAA+AGG | 0.418252 | 8.3:-43005736 | MS.gene25476:CDS |
| AAATATCAACCTCTTATCAT+TGG | 0.420551 | 8.3:+43005059 | None:intergenic |
| GTGGAGTGAGAAATTCTATA+GGG | 0.424894 | 8.3:-43005518 | MS.gene25476:CDS |
| GTTCATTGACCAATGATAAG+AGG | 0.426335 | 8.3:-43005068 | MS.gene25476:CDS |
| GAGTTTCTATGGTTGATAAA+GGG | 0.435292 | 8.3:+43005759 | None:intergenic |
| AAATGTCTGAGTTTACAATA+AGG | 0.441417 | 8.3:-43004792 | MS.gene25476:CDS |
| TTATACCACACCACCATTGC+AGG | 0.447302 | 8.3:-43004524 | MS.gene25476:CDS |
| GAGACACCGAGGCGGCGGCC+TGG | 0.448368 | 8.3:-43004730 | MS.gene25476:CDS |
| AGGTACTTTGTAGAAGGTCT+TGG | 0.451368 | 8.3:+43005654 | None:intergenic |
| TCAAAAGCAAGAGAAGTTAA+GGG | 0.452923 | 8.3:-43005241 | MS.gene25476:CDS |
| TCTAGTAAGAAAATTGTTGT+TGG | 0.460554 | 8.3:+43004933 | None:intergenic |
| AACATGCCAGGCCGCCGCCT+CGG | 0.461235 | 8.3:+43004724 | None:intergenic |
| CAGAAACTCTAACAGCTTTG+TGG | 0.461380 | 8.3:+43004686 | None:intergenic |
| TTGTTTCCTTTCTCTGAGGA+AGG | 0.475302 | 8.3:+43005634 | None:intergenic |
| AGTAAGAAAATTGTTGTTGG+AGG | 0.489605 | 8.3:+43004936 | None:intergenic |
| TAAAACTGATTTCACTTGCA+TGG | 0.493162 | 8.3:+43005403 | None:intergenic |
| CATTCTATGCTTGCCTCACT+CGG | 0.500118 | 8.3:+43004862 | None:intergenic |
| GCGCGTAACAATTCGGGCTA+AGG | 0.501756 | 8.3:+43004884 | None:intergenic |
| GTAAGAAAATTGTTGTTGGA+GGG | 0.511679 | 8.3:+43004937 | None:intergenic |
| AAAGTACCTTCCTCAGAGAA+AGG | 0.512613 | 8.3:-43005640 | MS.gene25476:CDS |
| TTGAGTAAGATTTCTGAGCT+AGG | 0.514673 | 8.3:+43005279 | None:intergenic |
| TTGCATCCCAACAACATGCC+AGG | 0.520880 | 8.3:+43004712 | None:intergenic |
| GTGCAAAAGGCAAAGAGGTA+TGG | 0.521742 | 8.3:-43004257 | MS.gene25476:CDS |
| TGAGGAAGGTACTTTGTAGA+AGG | 0.524612 | 8.3:+43005648 | None:intergenic |
| CCATACTTGAATATGAAAGA+AGG | 0.535861 | 8.3:-43005825 | MS.gene25476:CDS |
| GTGAGAAATTCTATAGGGAA+TGG | 0.537374 | 8.3:-43005513 | MS.gene25476:CDS |
| TTTGAGATTGAGAGTCTCAA+AGG | 0.537828 | 8.3:-43004964 | MS.gene25476:CDS |
| AAACTCCCCTCTTCATCAAG+TGG | 0.542981 | 8.3:-43005742 | MS.gene25476:CDS |
| CGAGTGAGGCAAGCATAGAA+TGG | 0.547758 | 8.3:-43004861 | MS.gene25476:CDS |
| GTTGACACAGAACTAATACT+TGG | 0.553240 | 8.3:+43005570 | None:intergenic |
| AGAAAACAGCATCATGGAGG+TGG | 0.557647 | 8.3:+43005850 | None:intergenic |
| CTAAAGAGACACCGAGGCGG+CGG | 0.557969 | 8.3:-43004735 | MS.gene25476:CDS |
| TTGGAGTTCCATACTCAACT+TGG | 0.566689 | 8.3:+43005589 | None:intergenic |
| ATGGAGAGAAAACAGCATCA+TGG | 0.576053 | 8.3:+43005844 | None:intergenic |
| AAACAATACTAATTCTCAAG+TGG | 0.581231 | 8.3:-43004602 | MS.gene25476:CDS |
| ATACTAATTCTCAAGTGGCA+AGG | 0.582567 | 8.3:-43004597 | MS.gene25476:CDS |
| AAGCAAGAGAAGTTAAGGGT+AGG | 0.593464 | 8.3:-43005237 | MS.gene25476:CDS |
| TATAGTAACTTTGAAGCATG+TGG | 0.603295 | 8.3:+43004490 | None:intergenic |
| GAGTTAGAGGCCTGCAATGG+TGG | 0.606643 | 8.3:+43004514 | None:intergenic |
| TCCTCAGAGAAAGGAAACAA+TGG | 0.613500 | 8.3:-43005631 | MS.gene25476:CDS |
| AAAAGGCATGGACAACAACA+TGG | 0.618653 | 8.3:-43004547 | MS.gene25476:CDS |
| CAAAATGCACTCTTAAAGAG+TGG | 0.623280 | 8.3:-43005537 | MS.gene25476:CDS |
| GCCATTGTTTCCTTTCTCTG+AGG | 0.638901 | 8.3:+43005630 | None:intergenic |
| GTATTAGTTCTGTGTCAACA+TGG | 0.647005 | 8.3:-43005566 | MS.gene25476:CDS |
| AGAGGCCTGCAATGGTGGTG+TGG | 0.651196 | 8.3:+43004519 | None:intergenic |
| GAATTGTTACGCGCCGAGTG+AGG | 0.653973 | 8.3:-43004875 | MS.gene25476:CDS |
| GTATAGTGCAAAAGGCAAAG+AGG | 0.661197 | 8.3:-43004262 | MS.gene25476:intron |
| AAGGACACCATTGAAACCAA+AGG | 0.662052 | 8.3:-43004761 | MS.gene25476:CDS |
| GGCGAGTTAGAGGCCTGCAA+TGG | 0.698179 | 8.3:+43004511 | None:intergenic |
| GAGAGAAAACAGCATCATGG+AGG | 0.723218 | 8.3:+43005847 | None:intergenic |
| TCGCTAAAGAGACACCGAGG+CGG | 0.748620 | 8.3:-43004738 | MS.gene25476:CDS |
| GGCAAGCATAGAATGGAGTG+TGG | 0.753377 | 8.3:-43004854 | MS.gene25476:CDS |
| AGGTCGCTAAAGAGACACCG+AGG | 0.789401 | 8.3:-43004741 | MS.gene25476:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAACAAAACTTCTAAGTA+TGG | - | chr8.3:43004752-43004771 | MS.gene25476:CDS | 20.0% |
| !! | TTATTGATCAATATTGGATT+TGG | + | chr8.3:43005037-43005056 | None:intergenic | 20.0% |
| !!! | AGACTTTTTTTTTGTTGTTT+TGG | - | chr8.3:43005828-43005847 | MS.gene25476:CDS | 20.0% |
| !!! | CTTTTGTTATTGATCAATAT+TGG | + | chr8.3:43005043-43005062 | None:intergenic | 20.0% |
| !!! | TTATCAATTTCTTCCATTTT+TGG | + | chr8.3:43005100-43005119 | None:intergenic | 20.0% |
| ! | AAACAATACTAATTCTCAAG+TGG | - | chr8.3:43005531-43005550 | MS.gene25476:CDS | 25.0% |
| ! | AAATATCAACCTCTTATCAT+TGG | + | chr8.3:43005077-43005096 | None:intergenic | 25.0% |
| ! | AAATGTCTGAGTTTACAATA+AGG | - | chr8.3:43005341-43005360 | MS.gene25476:CDS | 25.0% |
| ! | AAGCAATGAAAAGAATAGTT+TGG | - | chr8.3:43004922-43004941 | MS.gene25476:CDS | 25.0% |
| ! | AGACAAATTATGGTTCAAAA+AGG | - | chr8.3:43005569-43005588 | MS.gene25476:CDS | 25.0% |
| ! | ATGAATTAGTATAGTGCAAA+AGG | - | chr8.3:43005863-43005882 | MS.gene25476:CDS | 25.0% |
| ! | GGAAAGTTTATAGCAAAAAT+TGG | - | chr8.3:43004329-43004348 | MS.gene25476:intron | 25.0% |
| ! | TCTAGTAAGAAAATTGTTGT+TGG | + | chr8.3:43005203-43005222 | None:intergenic | 25.0% |
| !! | AATACTTCAAGTGATTTTCT+AGG | + | chr8.3:43005007-43005026 | None:intergenic | 25.0% |
| !! | AGAAAATCACTTGAAGTATT+TGG | - | chr8.3:43005007-43005026 | MS.gene25476:CDS | 25.0% |
| !!! | GAAATTGATAATGCAAACTA+TGG | - | chr8.3:43005109-43005128 | MS.gene25476:CDS | 25.0% |
| !!! | TTGTTTCTGCTACTAATTTT+TGG | + | chr8.3:43005511-43005530 | None:intergenic | 25.0% |
| AATTATGGTTCAAAAAGGCA+TGG | - | chr8.3:43005574-43005593 | MS.gene25476:CDS | 30.0% | |
| CCATACTTGAATATGAAAGA+AGG | - | chr8.3:43004308-43004327 | MS.gene25476:intron | 30.0% | |
| CCTTCTTTCATATTCAAGTA+TGG | + | chr8.3:43004311-43004330 | None:intergenic | 30.0% | |
| GCTATGTAATACAAACACTT+CGG | - | chr8.3:43005682-43005701 | MS.gene25476:CDS | 30.0% | |
| GCTGAAAAATACTTCAATAG+AGG | - | chr8.3:43004443-43004462 | MS.gene25476:intron | 30.0% | |
| TCAAAAGCAAGAGAAGTTAA+GGG | - | chr8.3:43004892-43004911 | MS.gene25476:CDS | 30.0% | |
| TGATAAAGGGATTGATTGTT+TGG | + | chr8.3:43004364-43004383 | None:intergenic | 30.0% | |
| TTACAATAAGGAGTCCAATA+AGG | - | chr8.3:43005353-43005372 | MS.gene25476:CDS | 30.0% | |
| TTCAAAAGCAAGAGAAGTTA+AGG | - | chr8.3:43004891-43004910 | MS.gene25476:CDS | 30.0% | |
| ! | AGTAAGAAAATTGTTGTTGG+AGG | + | chr8.3:43005200-43005219 | None:intergenic | 30.0% |
| ! | GTAAGAAAATTGTTGTTGGA+GGG | + | chr8.3:43005199-43005218 | None:intergenic | 30.0% |
| ! | TAAAACTGATTTCACTTGCA+TGG | + | chr8.3:43004733-43004752 | None:intergenic | 30.0% |
| !! | GAGGTTGATATTTCCAAAAA+TGG | - | chr8.3:43005084-43005103 | MS.gene25476:CDS | 30.0% |
| !! | GAGTTTCTATGGTTGATAAA+GGG | + | chr8.3:43004377-43004396 | None:intergenic | 30.0% |
| !! | TATAGTAACTTTGAAGCATG+TGG | + | chr8.3:43005646-43005665 | None:intergenic | 30.0% |
| !!! | CCAAGATTGAACACTTTTTT+CGG | + | chr8.3:43004800-43004819 | None:intergenic | 30.0% |
| AAATGTAACCACATGTGTCT+CGG | + | chr8.3:43005739-43005758 | None:intergenic | 35.0% | |
| AATGGCAAAGAAAAAGAGCA+TGG | - | chr8.3:43004638-43004657 | MS.gene25476:CDS | 35.0% | |
| AGTGGAGTGAGAAATTCTAT+AGG | - | chr8.3:43004614-43004633 | MS.gene25476:CDS | 35.0% | |
| ATACTAATTCTCAAGTGGCA+AGG | - | chr8.3:43005536-43005555 | MS.gene25476:CDS | 35.0% | |
| CAAAATGCACTCTTAAAGAG+TGG | - | chr8.3:43004596-43004615 | MS.gene25476:CDS | 35.0% | |
| GTGAGAAATTCTATAGGGAA+TGG | - | chr8.3:43004620-43004639 | MS.gene25476:CDS | 35.0% | |
| GTGGAGTGAGAAATTCTATA+GGG | - | chr8.3:43004615-43004634 | MS.gene25476:CDS | 35.0% | |
| GTTCATTGACCAATGATAAG+AGG | - | chr8.3:43005065-43005084 | MS.gene25476:CDS | 35.0% | |
| GTTGACACAGAACTAATACT+TGG | + | chr8.3:43004566-43004585 | None:intergenic | 35.0% | |
| TACAAACACTTCGGATTTGA+AGG | - | chr8.3:43005691-43005710 | MS.gene25476:CDS | 35.0% | |
| TTGAGTAAGATTTCTGAGCT+AGG | + | chr8.3:43004857-43004876 | None:intergenic | 35.0% | |
| TTTCCAGGAGAGACAAATTA+TGG | - | chr8.3:43005559-43005578 | MS.gene25476:CDS | 35.0% | |
| ! | GCAGAAGAAATTGGAGTATT+TGG | - | chr8.3:43004419-43004438 | MS.gene25476:intron | 35.0% |
| ! | GGTGTCTTTTCATCATCTAA+TGG | + | chr8.3:43004986-43005005 | None:intergenic | 35.0% |
| ! | TTTGAGATTGAGAGTCTCAA+AGG | - | chr8.3:43005169-43005188 | MS.gene25476:CDS | 35.0% |
| !! | AGAAAGTACCAAGTTGAGTA+TGG | - | chr8.3:43004536-43004555 | MS.gene25476:CDS | 35.0% |
| !! | CACTCTTTAAGAGTGCATTT+TGG | + | chr8.3:43004598-43004617 | None:intergenic | 35.0% |
| !! | CCGAAAAAAGTGTTCAATCT+TGG | - | chr8.3:43004797-43004816 | MS.gene25476:CDS | 35.0% |
| !! | GGAGTTTCTATGGTTGATAA+AGG | + | chr8.3:43004378-43004397 | None:intergenic | 35.0% |
| !! | GTATTAGTTCTGTGTCAACA+TGG | - | chr8.3:43004567-43004586 | MS.gene25476:CDS | 35.0% |
| AAATGTGTTAGTGTCGTGTC+TGG | - | chr8.3:43005776-43005795 | MS.gene25476:CDS | 40.0% | |
| AAGCAAGAGAAGTTAAGGGT+AGG | - | chr8.3:43004896-43004915 | MS.gene25476:CDS | 40.0% | |
| AAGGACACCATTGAAACCAA+AGG | - | chr8.3:43005372-43005391 | MS.gene25476:CDS | 40.0% | |
| AAGGTGTGACTATTAGTGTC+TGG | - | chr8.3:43005710-43005729 | MS.gene25476:CDS | 40.0% | |
| AGGTACTTTGTAGAAGGTCT+TGG | + | chr8.3:43004482-43004501 | None:intergenic | 40.0% | |
| ATGGAGAGAAAACAGCATCA+TGG | + | chr8.3:43004292-43004311 | None:intergenic | 40.0% | |
| CACAAAGCTGTTAGAGTTTC+TGG | - | chr8.3:43005448-43005467 | MS.gene25476:CDS | 40.0% | |
| CAGAAACTCTAACAGCTTTG+TGG | + | chr8.3:43005450-43005469 | None:intergenic | 40.0% | |
| GGTTTCAATGGTGTCCTTAT+TGG | + | chr8.3:43005370-43005389 | None:intergenic | 40.0% | |
| GTATAGTGCAAAAGGCAAAG+AGG | - | chr8.3:43005871-43005890 | MS.gene25476:CDS | 40.0% | |
| GTGGAAAAGGCAGAAGAAAT+TGG | - | chr8.3:43004410-43004429 | MS.gene25476:intron | 40.0% | |
| TCCTCAGAGAAAGGAAACAA+TGG | - | chr8.3:43004502-43004521 | MS.gene25476:CDS | 40.0% | |
| TCTAAGTATGGTGCAGTTCA+AGG | - | chr8.3:43004764-43004783 | MS.gene25476:CDS | 40.0% | |
| TTGGAGTTCCATACTCAACT+TGG | + | chr8.3:43004547-43004566 | None:intergenic | 40.0% | |
| ! | AAAAGGCATGGACAACAACA+TGG | - | chr8.3:43005586-43005605 | MS.gene25476:CDS | 40.0% |
| ! | AAAGTACCTTCCTCAGAGAA+AGG | - | chr8.3:43004493-43004512 | MS.gene25476:intron | 40.0% |
| ! | CCTTTTCCACTTGATGAAGA+GGG | + | chr8.3:43004400-43004419 | None:intergenic | 40.0% |
| ! | CTTTTCCACTTGATGAAGAG+GGG | + | chr8.3:43004399-43004418 | None:intergenic | 40.0% |
| ! | GAACCATAATTTGTCTCTCC+TGG | + | chr8.3:43005565-43005584 | None:intergenic | 40.0% |
| ! | TTAGCGACCTTTGGTTTCAA+TGG | + | chr8.3:43005382-43005401 | None:intergenic | 40.0% |
| ! | TTGTTTCCTTTCTCTGAGGA+AGG | + | chr8.3:43004502-43004521 | None:intergenic | 40.0% |
| !! | TGAGGAAGGTACTTTGTAGA+AGG | + | chr8.3:43004488-43004507 | None:intergenic | 40.0% |
| AAACTCCCCTCTTCATCAAG+TGG | - | chr8.3:43004391-43004410 | MS.gene25476:intron | 45.0% | |
| AGAAAACAGCATCATGGAGG+TGG | + | chr8.3:43004286-43004305 | None:intergenic | 45.0% | |
| CATTCTATGCTTGCCTCACT+CGG | + | chr8.3:43005274-43005293 | None:intergenic | 45.0% | |
| CCCTCTTCATCAAGTGGAAA+AGG | - | chr8.3:43004397-43004416 | MS.gene25476:intron | 45.0% | |
| GAGAGAAAACAGCATCATGG+AGG | + | chr8.3:43004289-43004308 | None:intergenic | 45.0% | |
| GATGAAGAGGGGAGTTTCTA+TGG | + | chr8.3:43004388-43004407 | None:intergenic | 45.0% | |
| GCCATTGTTTCCTTTCTCTG+AGG | + | chr8.3:43004506-43004525 | None:intergenic | 45.0% | |
| GTGCAAAAGGCAAAGAGGTA+TGG | - | chr8.3:43005876-43005895 | MS.gene25476:CDS | 45.0% | |
| TTATACCACACCACCATTGC+AGG | - | chr8.3:43005609-43005628 | MS.gene25476:CDS | 45.0% | |
| ! | GCCTTTTCCACTTGATGAAG+AGG | + | chr8.3:43004401-43004420 | None:intergenic | 45.0% |
| CGAGTGAGGCAAGCATAGAA+TGG | - | chr8.3:43005272-43005291 | MS.gene25476:CDS | 50.0% | |
| GGCAAGCATAGAATGGAGTG+TGG | - | chr8.3:43005279-43005298 | MS.gene25476:CDS | 50.0% | |
| GGTGTCTCTTTAGCGACCTT+TGG | + | chr8.3:43005391-43005410 | None:intergenic | 50.0% | |
| TCACTCGGCGCGTAACAATT+CGG | + | chr8.3:43005259-43005278 | None:intergenic | 50.0% | |
| TCTCAAGTGGCAAGGTTTCC+AGG | - | chr8.3:43005544-43005563 | MS.gene25476:CDS | 50.0% | |
| TCTGGTATCCGAGACACATG+TGG | - | chr8.3:43005728-43005747 | MS.gene25476:CDS | 50.0% | |
| TGAAGCATGTGGCGAGTTAG+AGG | + | chr8.3:43005635-43005654 | None:intergenic | 50.0% | |
| TTGCATCCCAACAACATGCC+AGG | + | chr8.3:43005424-43005443 | None:intergenic | 50.0% | |
| AGGTCGCTAAAGAGACACCG+AGG | - | chr8.3:43005392-43005411 | MS.gene25476:CDS | 55.0% | |
| CACTCGGCGCGTAACAATTC+GGG | + | chr8.3:43005258-43005277 | None:intergenic | 55.0% | |
| GAATTGTTACGCGCCGAGTG+AGG | - | chr8.3:43005258-43005277 | MS.gene25476:CDS | 55.0% | |
| GAGTTAGAGGCCTGCAATGG+TGG | + | chr8.3:43005622-43005641 | None:intergenic | 55.0% | |
| GCGCGTAACAATTCGGGCTA+AGG | + | chr8.3:43005252-43005271 | None:intergenic | 55.0% | |
| TCGCTAAAGAGACACCGAGG+CGG | - | chr8.3:43005395-43005414 | MS.gene25476:CDS | 55.0% | |
| CTAAAGAGACACCGAGGCGG+CGG | - | chr8.3:43005398-43005417 | MS.gene25476:CDS | 60.0% | |
| GGCGAGTTAGAGGCCTGCAA+TGG | + | chr8.3:43005625-43005644 | None:intergenic | 60.0% | |
| ! | AGAGGCCTGCAATGGTGGTG+TGG | + | chr8.3:43005617-43005636 | None:intergenic | 60.0% |
| CGGCGGCCTGGCATGTTGTT+GGG | - | chr8.3:43005415-43005434 | MS.gene25476:CDS | 65.0% | |
| AACATGCCAGGCCGCCGCCT+CGG | + | chr8.3:43005412-43005431 | None:intergenic | 70.0% | |
| GCGGCGGCCTGGCATGTTGT+TGG | - | chr8.3:43005414-43005433 | MS.gene25476:CDS | 70.0% | |
| ! | GAGACACCGAGGCGGCGGCC+TGG | - | chr8.3:43005403-43005422 | MS.gene25476:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 43004245 | 43005910 | 43004245 | ID=MS.gene25476 |
| chr8.3 | mRNA | 43004245 | 43005910 | 43004245 | ID=MS.gene25476.t1;Parent=MS.gene25476 |
| chr8.3 | exon | 43004499 | 43005910 | 43004499 | ID=MS.gene25476.t1.exon1;Parent=MS.gene25476.t1 |
| chr8.3 | CDS | 43004499 | 43005910 | 43004499 | ID=cds.MS.gene25476.t1;Parent=MS.gene25476.t1 |
| chr8.3 | exon | 43004245 | 43004278 | 43004245 | ID=MS.gene25476.t1.exon2;Parent=MS.gene25476.t1 |
| chr8.3 | CDS | 43004245 | 43004278 | 43004245 | ID=cds.MS.gene25476.t1;Parent=MS.gene25476.t1 |
| Gene Sequence |
| Protein sequence |
