AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55815


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55815.t1 MTR_8g099615 95.000 140 7 0 1 140 1 140 5.36e-98 280
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55815.t1 AT5G42070 78.571 70 15 0 65 134 69 138 7.90e-36 122

Find 47 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCTCTACGTTCAATTAA+AGG 0.240046 4.3:-4411352 MS.gene55815:CDS
TGTCCTGGATTTGTGGCTTC+TGG 0.277176 4.3:-4411767 MS.gene55815:CDS
GGTGGTATTGCAACAGTTCC+TGG 0.294401 4.3:-4411824 MS.gene55815:CDS
CATGGCCTGTATGCCTTTAT+AGG 0.312442 4.3:+4411788 None:intergenic
CTTTGGCTGGTGGCCTATAA+AGG 0.340571 4.3:-4411801 MS.gene55815:CDS
ATTGCAACAGTTCCTGGCTT+TGG 0.355448 4.3:-4411818 MS.gene55815:CDS
CAGGCCATGTCCTGGATTTG+TGG 0.362074 4.3:-4411774 MS.gene55815:CDS
CAACAGTTCCTGGCTTTGGC+TGG 0.392114 4.3:-4411814 MS.gene55815:CDS
CACCTCTACGTTCAATTAAA+GGG 0.424582 4.3:-4411351 MS.gene55815:CDS
CAGTTCCTGGCTTTGGCTGG+TGG 0.448784 4.3:-4411811 MS.gene55815:CDS
GCAGAAGAAATGAGTGAAGT+TGG 0.456714 4.3:+4412042 None:intergenic
CAATGGTGAGGGTAGGATGC+AGG 0.456901 4.3:-4411846 MS.gene55815:CDS
GAAACAGCAAGTTCATCATC+TGG 0.480522 4.3:+4412018 None:intergenic
GACGAGGTTGTCTCCGGTAG+TGG 0.489301 4.3:-4411713 MS.gene55815:CDS
CCACCAGAAGCCACAAATCC+AGG 0.502695 4.3:+4411764 None:intergenic
GACTTGGAGTCAGTTACAGT+TGG 0.504211 4.3:+4411677 None:intergenic
TGGATGGTACCTCTGTTTAG+TGG 0.504482 4.3:+4411978 None:intergenic
AAGGCATACAGGCCATGTCC+TGG 0.519447 4.3:-4411782 MS.gene55815:CDS
GGAACAAATATCTTAGTAGC+TGG 0.533811 4.3:+4412081 None:intergenic
TGGTGGTAGGTACAGACGAC+AGG 0.534138 4.3:-4411747 MS.gene55815:CDS
GATTTGTGGCTTCTGGTGGT+AGG 0.534194 4.3:-4411760 MS.gene55815:CDS
ATAGGCCACCAGCCAAAGCC+AGG 0.536722 4.3:+4411806 None:intergenic
TCTAGTTAGTTCAAATGAGA+AGG 0.537196 4.3:-4411538 MS.gene55815:CDS
AGCATGGACGAGGTTGTCTC+CGG 0.542240 4.3:-4411719 MS.gene55815:CDS
GAAGTTGGAGAGCATAGTGT+GGG 0.545271 4.3:+4412057 None:intergenic
GGAAACGAAGAAATTGAGAA+AGG 0.546629 4.3:-4411872 MS.gene55815:CDS
TGAAGTTGGAGAGCATAGTG+TGG 0.558341 4.3:+4412056 None:intergenic
CCTGGATTTGTGGCTTCTGG+TGG 0.563457 4.3:-4411764 MS.gene55815:CDS
GAAATTGAGAAAGGATGCAA+TGG 0.563789 4.3:-4411863 MS.gene55815:CDS
TGATGATAATGTTGAGGTGT+TGG 0.564881 4.3:+4412135 None:intergenic
AAGGATGCAATGGTGAGGGT+AGG 0.574706 4.3:-4411853 MS.gene55815:CDS
AATGGTGAGGGTAGGATGCA+GGG 0.575709 4.3:-4411845 MS.gene55815:CDS
CAGACGACAGGGACAAAGCA+TGG 0.576125 4.3:-4411735 MS.gene55815:CDS
GGTGGCCTATAAAGGCATAC+AGG 0.580795 4.3:-4411793 MS.gene55815:CDS
GAGAAAGGATGCAATGGTGA+GGG 0.581838 4.3:-4411857 MS.gene55815:CDS
AGACAATAGATACCTTGACT+TGG 0.607021 4.3:+4411661 None:intergenic
TGAGAAAGGATGCAATGGTG+AGG 0.617666 4.3:-4411858 MS.gene55815:CDS
GTTGGAGAGCATAGTGTGGG+AGG 0.638611 4.3:+4412060 None:intergenic
AACAGCGGTTGCAAAGCATG+TGG 0.642827 4.3:-4411893 MS.gene55815:intron
CTGTAACTGACTCCAAGTCA+AGG 0.645140 4.3:-4411673 MS.gene55815:intron
ACAGGGACAAAGCATGGACG+AGG 0.654227 4.3:-4411729 MS.gene55815:CDS
AGCCCTTTAATTGAACGTAG+AGG 0.660275 4.3:+4411349 None:intergenic
TGAACGTGATGATAATGTTG+AGG 0.683199 4.3:+4412129 None:intergenic
GAAGCCACAAATCCAGGACA+TGG 0.686791 4.3:+4411770 None:intergenic
GGTGGTAGGTACAGACGACA+GGG 0.692150 4.3:-4411746 MS.gene55815:CDS
CAGAATTCTCCACTAAACAG+AGG 0.708713 4.3:-4411987 MS.gene55815:intron
GGTGAGGGTAGGATGCAGGG+TGG 0.740434 4.3:-4411842 MS.gene55815:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAAAATAAAAAATA+GGG + chr4.3:4412048-4412067 None:intergenic 0.0%
!! AAGATTTATCTATAAATAAA+TGG + chr4.3:4411899-4411918 None:intergenic 10.0%
!!! AAAGATTTTTATTTAATGAA+GGG + chr4.3:4411557-4411576 None:intergenic 10.0%
!! CTTCATTAAATAAAAATCTT+TGG - chr4.3:4411556-4411575 MS.gene55815:CDS 15.0%
!!! AAGATTTTTATTTAATGAAG+GGG + chr4.3:4411556-4411575 None:intergenic 15.0%
!!! CAAAGATTTTTATTTAATGA+AGG + chr4.3:4411558-4411577 None:intergenic 15.0%
!! TCTATAAATAAATGGTAAAC+TGG + chr4.3:4411891-4411910 None:intergenic 20.0%
!!! ATTTTGTTTATGTTGATAGA+AGG - chr4.3:4412009-4412028 MS.gene55815:CDS 20.0%
!!! TTTTGTTTATGTTGATAGAA+GGG - chr4.3:4412010-4412029 MS.gene55815:CDS 20.0%
! TGTACAAACCAAAAAAATCA+TGG - chr4.3:4411400-4411419 MS.gene55815:intron 25.0%
! TTATTTAATGAAGGGGAAAA+TGG + chr4.3:4411549-4411568 None:intergenic 25.0%
!! TTTGTTTATGTTGATAGAAG+GGG - chr4.3:4412011-4412030 MS.gene55815:CDS 25.0%
!!! ATTTTTTATTTTGGTTCGCA+AGG - chr4.3:4412056-4412075 MS.gene55815:CDS 25.0%
!!! CAAATGAGAAGGATTTTAAT+AGG - chr4.3:4411977-4411996 MS.gene55815:intron 25.0%
!!! TTTTTTATTTTGGTTCGCAA+GGG - chr4.3:4412057-4412076 MS.gene55815:CDS 25.0%
CTTGATTCTAAGTCTTTCTT+AGG - chr4.3:4412129-4412148 MS.gene55815:CDS 30.0%
TCTAGTTAGTTCAAATGAGA+AGG - chr4.3:4411966-4411985 MS.gene55815:intron 30.0%
TGTCTCTTTCTCATGTATAT+GGG - chr4.3:4411861-4411880 MS.gene55815:CDS 30.0%
TTGTCTCTTTCTCATGTATA+TGG - chr4.3:4411860-4411879 MS.gene55815:CDS 30.0%
!!! TGTTTTAATTTTGCCACTAC+CGG + chr4.3:4411807-4411826 None:intergenic 30.0%
!!! TTTTTATTTTGGTTCGCAAG+GGG - chr4.3:4412058-4412077 MS.gene55815:CDS 30.0%
AGACAATAGATACCTTGACT+TGG + chr4.3:4411846-4411865 None:intergenic 35.0%
CACCTCTACGTTCAATTAAA+GGG - chr4.3:4412153-4412172 MS.gene55815:CDS 35.0%
GAAATTGAGAAAGGATGCAA+TGG - chr4.3:4411641-4411660 MS.gene55815:intron 35.0%
GGAAACGAAGAAATTGAGAA+AGG - chr4.3:4411632-4411651 MS.gene55815:intron 35.0%
GGAACAAATATCTTAGTAGC+TGG + chr4.3:4411426-4411445 None:intergenic 35.0%
TCACCTCTACGTTCAATTAA+AGG - chr4.3:4412152-4412171 MS.gene55815:CDS 35.0%
TTAATGAAGGGGAAAATGGA+TGG + chr4.3:4411545-4411564 None:intergenic 35.0%
! AAGCTGCATCAAGACTTTTA+AGG - chr4.3:4411942-4411961 MS.gene55815:intron 35.0%
! AGCTGCATCAAGACTTTTAA+GGG - chr4.3:4411943-4411962 MS.gene55815:intron 35.0%
! TGAACGTGATGATAATGTTG+AGG + chr4.3:4411378-4411397 None:intergenic 35.0%
! TGATGATAATGTTGAGGTGT+TGG + chr4.3:4411372-4411391 None:intergenic 35.0%
AGCCCTTTAATTGAACGTAG+AGG + chr4.3:4412158-4412177 None:intergenic 40.0%
CAGAATTCTCCACTAAACAG+AGG - chr4.3:4411517-4411536 MS.gene55815:intron 40.0%
GAAACAGCAAGTTCATCATC+TGG + chr4.3:4411489-4411508 None:intergenic 40.0%
GCAGAAGAAATGAGTGAAGT+TGG + chr4.3:4411465-4411484 None:intergenic 40.0%
TTTGAGTAGTTGCTGAACAG+CGG - chr4.3:4411596-4411615 MS.gene55815:intron 40.0%
!!! GTAGCTGGCCATGATTTTTT+TGG + chr4.3:4411411-4411430 None:intergenic 40.0%
ATTGCAACAGTTCCTGGCTT+TGG - chr4.3:4411686-4411705 MS.gene55815:CDS 45.0%
CATGGCCTGTATGCCTTTAT+AGG + chr4.3:4411719-4411738 None:intergenic 45.0%
GACTTGGAGTCAGTTACAGT+TGG + chr4.3:4411830-4411849 None:intergenic 45.0%
GAGAAAGGATGCAATGGTGA+GGG - chr4.3:4411647-4411666 MS.gene55815:intron 45.0%
TGAGAAAGGATGCAATGGTG+AGG - chr4.3:4411646-4411665 MS.gene55815:intron 45.0%
TGGATGGTACCTCTGTTTAG+TGG + chr4.3:4411529-4411548 None:intergenic 45.0%
! CTGTAACTGACTCCAAGTCA+AGG - chr4.3:4411831-4411850 MS.gene55815:CDS 45.0%
! GAAGTTGGAGAGCATAGTGT+GGG + chr4.3:4411450-4411469 None:intergenic 45.0%
! TGAAGTTGGAGAGCATAGTG+TGG + chr4.3:4411451-4411470 None:intergenic 45.0%
!! CAAAATAAAAAATAAAAAAT+AGG + chr4.3:4412049-4412068 None:intergenic 5.0%
!!! CTATTTTTTATTTTTTATTT+TGG - chr4.3:4412047-4412066 MS.gene55815:CDS 5.0%
AACAGCGGTTGCAAAGCATG+TGG - chr4.3:4411611-4411630 MS.gene55815:intron 50.0%
AAGGATGCAATGGTGAGGGT+AGG - chr4.3:4411651-4411670 MS.gene55815:intron 50.0%
AATGGTGAGGGTAGGATGCA+GGG - chr4.3:4411659-4411678 MS.gene55815:intron 50.0%
CTTTGGCTGGTGGCCTATAA+AGG - chr4.3:4411703-4411722 MS.gene55815:CDS 50.0%
GAAGCCACAAATCCAGGACA+TGG + chr4.3:4411737-4411756 None:intergenic 50.0%
GATTTGTGGCTTCTGGTGGT+AGG - chr4.3:4411744-4411763 MS.gene55815:CDS 50.0%
GGTGGTATTGCAACAGTTCC+TGG - chr4.3:4411680-4411699 MS.gene55815:CDS 50.0%
TGTCCTGGATTTGTGGCTTC+TGG - chr4.3:4411737-4411756 MS.gene55815:CDS 50.0%
! GGTGGCCTATAAAGGCATAC+AGG - chr4.3:4411711-4411730 MS.gene55815:CDS 50.0%
AAGGCATACAGGCCATGTCC+TGG - chr4.3:4411722-4411741 MS.gene55815:CDS 55.0%
ACAGGGACAAAGCATGGACG+AGG - chr4.3:4411775-4411794 MS.gene55815:CDS 55.0%
AGCATGGACGAGGTTGTCTC+CGG - chr4.3:4411785-4411804 MS.gene55815:CDS 55.0%
CAATGGTGAGGGTAGGATGC+AGG - chr4.3:4411658-4411677 MS.gene55815:intron 55.0%
CAGACGACAGGGACAAAGCA+TGG - chr4.3:4411769-4411788 MS.gene55815:CDS 55.0%
CAGGCCATGTCCTGGATTTG+TGG - chr4.3:4411730-4411749 MS.gene55815:CDS 55.0%
CCACCAGAAGCCACAAATCC+AGG + chr4.3:4411743-4411762 None:intergenic 55.0%
CCTGGATTTGTGGCTTCTGG+TGG - chr4.3:4411740-4411759 MS.gene55815:CDS 55.0%
GGTGGTAGGTACAGACGACA+GGG - chr4.3:4411758-4411777 MS.gene55815:CDS 55.0%
TGGTGGTAGGTACAGACGAC+AGG - chr4.3:4411757-4411776 MS.gene55815:CDS 55.0%
! GTTGGAGAGCATAGTGTGGG+AGG + chr4.3:4411447-4411466 None:intergenic 55.0%
!! CAACAGTTCCTGGCTTTGGC+TGG - chr4.3:4411690-4411709 MS.gene55815:CDS 55.0%
ATAGGCCACCAGCCAAAGCC+AGG + chr4.3:4411701-4411720 None:intergenic 60.0%
GACGAGGTTGTCTCCGGTAG+TGG - chr4.3:4411791-4411810 MS.gene55815:CDS 60.0%
!! CAGTTCCTGGCTTTGGCTGG+TGG - chr4.3:4411693-4411712 MS.gene55815:CDS 60.0%
GGTGAGGGTAGGATGCAGGG+TGG - chr4.3:4411662-4411681 MS.gene55815:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 4411345 4412181 4411345 ID=MS.gene55815
chr4.3 mRNA 4411345 4412181 4411345 ID=MS.gene55815.t1;Parent=MS.gene55815
chr4.3 exon 4411988 4412181 4411988 ID=MS.gene55815.t1.exon1;Parent=MS.gene55815.t1
chr4.3 CDS 4411988 4412181 4411988 ID=cds.MS.gene55815.t1;Parent=MS.gene55815.t1
chr4.3 exon 4411674 4411910 4411674 ID=MS.gene55815.t1.exon2;Parent=MS.gene55815.t1
chr4.3 CDS 4411674 4411910 4411674 ID=cds.MS.gene55815.t1;Parent=MS.gene55815.t1
chr4.3 exon 4411528 4411581 4411528 ID=MS.gene55815.t1.exon3;Parent=MS.gene55815.t1
chr4.3 CDS 4411528 4411581 4411528 ID=cds.MS.gene55815.t1;Parent=MS.gene55815.t1
chr4.3 exon 4411345 4411375 4411345 ID=MS.gene55815.t1.exon4;Parent=MS.gene55815.t1
chr4.3 CDS 4411345 4411375 4411345 ID=cds.MS.gene55815.t1;Parent=MS.gene55815.t1
Gene Sequence

>MS.gene55815

ATGACAACAGCTTTCTTCACAGTTCCAACACCTCAACATTATCATCACGTTCAATTGTACAAACCAAAAAAATCATGGCCAGCTACTAAGATATTTGTTCCTCCCACACTATGCTCTCCAACTTCACTCATTTCTTCTGCACCAGATGATGAACTTGCTGTTTCTGATCAATCAGAATTCTCCACTAAACAGAGGTACCATCCATTTTCCCCTTCATTAAATAAAAATCTTTGGAGACAACAACATCTTATTTTGAGTAGTTGCTGAACAGCGGTTGCAAAGCATGTGGAAACGAAGAAATTGAGAAAGGATGCAATGGTGAGGGTAGGATGCAGGGTGGTATTGCAACAGTTCCTGGCTTTGGCTGGTGGCCTATAAAGGCATACAGGCCATGTCCTGGATTTGTGGCTTCTGGTGGTAGGTACAGACGACAGGGACAAAGCATGGACGAGGTTGTCTCCGGTAGTGGCAAAATTAAAACACCAACTGTAACTGACTCCAAGTCAAGGTATCTATTGTCTCTTTCTCATGTATATGGGCTATCCAGTTTACCATTTATTTATAGATAAATCTTTGTCTTACAACATATCATTCATGAAGCTGCATCAAGACTTTTAAGGGTCTAGTTAGTTCAAATGAGAAGGATTTTAATAGGTACATATTTATTTTGTTTATGTTGATAGAAGGGGAGACAACATAGCCCTATTTTTTATTTTTTATTTTGGTTCGCAAGGGGAGTGATTGATTTTAACTAGTAGCAAATTTACATTTAATATTCTTGTAGCTTGATTCTAAGTCTTTCTTAGGTCACCTCTACGTTCAATTAAAGGGCTATGA

Protein sequence

>MS.gene55815.t1

MTTAFFTVPTPQHYHHVQLYKPKKSWPATKIFVPPTLCSPTSLISSAPDDELAVSDQSEFSTKQSGCKACGNEEIEKGCNGEGRMQGGIATVPGFGWWPIKAYRPCPGFVASGGRYRRQGQSMDEVVSGSGKIKTPTVTDSKSSCIKTFKGLVSSNEKDFNRSPLRSIKGL