Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63787.t1 | KEH28429.1 | 90.1 | 81 | 5 | 1 | 1 | 81 | 1 | 78 | 6.70E-31 | 142.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63787.t1 | A0A072UFB0 | 90.1 | 81 | 5 | 1 | 1 | 81 | 1 | 78 | 4.8e-31 | 142.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene63787 | 0.83988 | 1.22E-57 | -1.69E-46 |
| MS.gene049171 | MS.gene63787 | 0.868161 | 8.02E-66 | -1.69E-46 |
| MS.gene049173 | MS.gene63787 | 0.831937 | 1.26E-55 | -1.69E-46 |
| MS.gene049217 | MS.gene63787 | 0.8739 | 1.03E-67 | -1.69E-46 |
| MS.gene049222 | MS.gene63787 | 0.854172 | 1.47E-61 | -1.69E-46 |
| MS.gene049285 | MS.gene63787 | 0.895844 | 6.51E-76 | -1.69E-46 |
| MS.gene049320 | MS.gene63787 | 0.878848 | 2.01E-69 | -1.69E-46 |
| MS.gene04939 | MS.gene63787 | 0.801021 | 1.10E-48 | -1.69E-46 |
| MS.gene049427 | MS.gene63787 | 0.858991 | 5.63E-63 | -1.69E-46 |
| MS.gene049609 | MS.gene63787 | 0.854244 | 1.40E-61 | -1.69E-46 |
| MS.gene049614 | MS.gene63787 | 0.83899 | 2.07E-57 | -1.69E-46 |
| MS.gene049627 | MS.gene63787 | 0.825116 | 5.58E-54 | -1.69E-46 |
| MS.gene049633 | MS.gene63787 | 0.817888 | 2.61E-52 | -1.69E-46 |
| MS.gene049805 | MS.gene63787 | 0.855097 | 7.93E-62 | -1.69E-46 |
| MS.gene050076 | MS.gene63787 | 0.82673 | 2.31E-54 | -1.69E-46 |
| MS.gene05010 | MS.gene63787 | 0.816065 | 6.70E-52 | -1.69E-46 |
| MS.gene050163 | MS.gene63787 | 0.887382 | 1.50E-72 | -1.69E-46 |
| MS.gene050165 | MS.gene63787 | 0.865796 | 4.55E-65 | -1.69E-46 |
| MS.gene050333 | MS.gene63787 | 0.853531 | 2.25E-61 | -1.69E-46 |
| MS.gene050432 | MS.gene63787 | 0.820354 | 7.17E-53 | -1.69E-46 |
| MS.gene050444 | MS.gene63787 | 0.809179 | 2.15E-50 | -1.69E-46 |
| MS.gene050475 | MS.gene63787 | -0.817369 | 3.42E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene63787 | 0.861933 | 7.24E-64 | -1.69E-46 |
| MS.gene050518 | MS.gene63787 | 0.857243 | 1.86E-62 | -1.69E-46 |
| MS.gene050538 | MS.gene63787 | 0.823856 | 1.11E-53 | -1.69E-46 |
| MS.gene050541 | MS.gene63787 | -0.813645 | 2.31E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene63787 | 0.826962 | 2.03E-54 | -1.69E-46 |
| MS.gene050593 | MS.gene63787 | 0.842564 | 2.39E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene63787 | 0.86428 | 1.36E-64 | -1.69E-46 |
| MS.gene050675 | MS.gene63787 | 0.842297 | 2.82E-58 | -1.69E-46 |
| MS.gene050699 | MS.gene63787 | 0.881803 | 1.77E-70 | -1.69E-46 |
| MS.gene050747 | MS.gene63787 | 0.834585 | 2.75E-56 | -1.69E-46 |
| MS.gene050767 | MS.gene63787 | 0.842899 | 1.95E-58 | -1.69E-46 |
| MS.gene050770 | MS.gene63787 | 0.849248 | 3.65E-60 | -1.69E-46 |
| MS.gene050772 | MS.gene63787 | 0.874262 | 7.75E-68 | -1.69E-46 |
| MS.gene050781 | MS.gene63787 | 0.802343 | 5.87E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene63787 | 0.802892 | 4.52E-49 | -1.69E-46 |
| MS.gene05081 | MS.gene63787 | 0.802452 | 5.57E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene63787 | 0.837248 | 5.81E-57 | -1.69E-46 |
| MS.gene050986 | MS.gene63787 | 0.83713 | 6.23E-57 | -1.69E-46 |
| MS.gene05100 | MS.gene63787 | 0.862619 | 4.46E-64 | -1.69E-46 |
| MS.gene05101 | MS.gene63787 | 0.801179 | 1.02E-48 | -1.69E-46 |
| MS.gene05105 | MS.gene63787 | 0.80781 | 4.21E-50 | -1.69E-46 |
| MS.gene051228 | MS.gene63787 | 0.824649 | 7.20E-54 | -1.69E-46 |
| MS.gene051317 | MS.gene63787 | 0.803005 | 4.29E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene63787 | 0.840046 | 1.10E-57 | -1.69E-46 |
| MS.gene051434 | MS.gene63787 | 0.815496 | 8.98E-52 | -1.69E-46 |
| MS.gene051551 | MS.gene63787 | 0.842791 | 2.08E-58 | -1.69E-46 |
| MS.gene051666 | MS.gene63787 | 0.844707 | 6.40E-59 | -1.69E-46 |
| MS.gene051692 | MS.gene63787 | 0.811384 | 7.19E-51 | -1.69E-46 |
| MS.gene051694 | MS.gene63787 | 0.844778 | 6.12E-59 | -1.69E-46 |
| MS.gene051810 | MS.gene63787 | 0.880058 | 7.50E-70 | -1.69E-46 |
| MS.gene051858 | MS.gene63787 | 0.868817 | 4.92E-66 | -1.69E-46 |
| MS.gene051949 | MS.gene63787 | 0.810834 | 9.46E-51 | -1.69E-46 |
| MS.gene052271 | MS.gene63787 | 0.900843 | 4.88E-78 | -1.69E-46 |
| MS.gene052290 | MS.gene63787 | 0.827115 | 1.87E-54 | -1.69E-46 |
| MS.gene052310 | MS.gene63787 | 0.805217 | 1.49E-49 | -1.69E-46 |
| MS.gene052322 | MS.gene63787 | 0.80341 | 3.53E-49 | -1.69E-46 |
| MS.gene052379 | MS.gene63787 | 0.880656 | 4.59E-70 | -1.69E-46 |
| MS.gene052421 | MS.gene63787 | 0.815876 | 7.38E-52 | -1.69E-46 |
| MS.gene052481 | MS.gene63787 | 0.817308 | 3.53E-52 | -1.69E-46 |
| MS.gene052482 | MS.gene63787 | 0.800041 | 1.74E-48 | -1.69E-46 |
| MS.gene052484 | MS.gene63787 | 0.838707 | 2.45E-57 | -1.69E-46 |
| MS.gene052486 | MS.gene63787 | 0.813323 | 2.71E-51 | -1.69E-46 |
| MS.gene052503 | MS.gene63787 | 0.805506 | 1.29E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene63787 | 0.873843 | 1.07E-67 | -1.69E-46 |
| MS.gene052823 | MS.gene63787 | 0.800834 | 1.20E-48 | -1.69E-46 |
| MS.gene052870 | MS.gene63787 | 0.827441 | 1.56E-54 | -1.69E-46 |
| MS.gene052872 | MS.gene63787 | 0.832488 | 9.19E-56 | -1.69E-46 |
| MS.gene052916 | MS.gene63787 | 0.817548 | 3.11E-52 | -1.69E-46 |
| MS.gene053009 | MS.gene63787 | 0.822849 | 1.90E-53 | -1.69E-46 |
| MS.gene053103 | MS.gene63787 | 0.829115 | 6.17E-55 | -1.69E-46 |
| MS.gene053104 | MS.gene63787 | 0.845995 | 2.87E-59 | -1.69E-46 |
| MS.gene053107 | MS.gene63787 | 0.818128 | 2.30E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene63787 | 0.816082 | 6.64E-52 | -1.69E-46 |
| MS.gene053207 | MS.gene63787 | 0.860631 | 1.81E-63 | -1.69E-46 |
| MS.gene053213 | MS.gene63787 | 0.896026 | 5.48E-76 | -1.69E-46 |
| MS.gene053286 | MS.gene63787 | 0.888528 | 5.45E-73 | -1.69E-46 |
| MS.gene053322 | MS.gene63787 | 0.90159 | 2.30E-78 | -1.69E-46 |
| MS.gene053436 | MS.gene63787 | 0.80737 | 5.23E-50 | -1.69E-46 |
| MS.gene053480 | MS.gene63787 | 0.809724 | 1.64E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene63787 | 0.831284 | 1.82E-55 | -1.69E-46 |
| MS.gene053579 | MS.gene63787 | 0.801307 | 9.58E-49 | -1.69E-46 |
| MS.gene053632 | MS.gene63787 | 0.861655 | 8.81E-64 | -1.69E-46 |
| MS.gene053824 | MS.gene63787 | 0.822238 | 2.64E-53 | -1.69E-46 |
| MS.gene053915 | MS.gene63787 | 0.83085 | 2.33E-55 | -1.69E-46 |
| MS.gene053916 | MS.gene63787 | 0.801274 | 9.73E-49 | -1.69E-46 |
| MS.gene054376 | MS.gene63787 | 0.835381 | 1.73E-56 | -1.69E-46 |
| MS.gene054408 | MS.gene63787 | 0.82491 | 6.25E-54 | -1.69E-46 |
| MS.gene054595 | MS.gene63787 | 0.828282 | 9.80E-55 | -1.69E-46 |
| MS.gene054600 | MS.gene63787 | 0.866037 | 3.82E-65 | -1.69E-46 |
| MS.gene054642 | MS.gene63787 | 0.897653 | 1.14E-76 | -1.69E-46 |
| MS.gene054802 | MS.gene63787 | 0.858726 | 6.76E-63 | -1.69E-46 |
| MS.gene054803 | MS.gene63787 | 0.855944 | 4.49E-62 | -1.69E-46 |
| MS.gene054804 | MS.gene63787 | 0.859591 | 3.72E-63 | -1.69E-46 |
| MS.gene054927 | MS.gene63787 | 0.802252 | 6.13E-49 | -1.69E-46 |
| MS.gene054954 | MS.gene63787 | 0.852601 | 4.15E-61 | -1.69E-46 |
| MS.gene05505 | MS.gene63787 | 0.830118 | 3.52E-55 | -1.69E-46 |
| MS.gene055154 | MS.gene63787 | -0.811848 | 5.70E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene63787 | 0.882801 | 7.68E-71 | -1.69E-46 |
| MS.gene055224 | MS.gene63787 | 0.800369 | 1.49E-48 | -1.69E-46 |
| MS.gene055289 | MS.gene63787 | 0.819487 | 1.13E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene63787 | 0.877772 | 4.81E-69 | -1.69E-46 |
| MS.gene055397 | MS.gene63787 | 0.856353 | 3.41E-62 | -1.69E-46 |
| MS.gene055412 | MS.gene63787 | 0.827111 | 1.87E-54 | -1.69E-46 |
| MS.gene055499 | MS.gene63787 | 0.873533 | 1.37E-67 | -1.69E-46 |
| MS.gene055502 | MS.gene63787 | 0.877816 | 4.64E-69 | -1.69E-46 |
| MS.gene055577 | MS.gene63787 | 0.844584 | 6.90E-59 | -1.69E-46 |
| MS.gene055636 | MS.gene63787 | 0.83639 | 9.62E-57 | -1.69E-46 |
| MS.gene055638 | MS.gene63787 | 0.828977 | 6.66E-55 | -1.69E-46 |
| MS.gene055647 | MS.gene63787 | 0.886265 | 3.97E-72 | -1.69E-46 |
| MS.gene055722 | MS.gene63787 | 0.853937 | 1.72E-61 | -1.69E-46 |
| MS.gene05592 | MS.gene63787 | 0.859721 | 3.40E-63 | -1.69E-46 |
| MS.gene055941 | MS.gene63787 | 0.850423 | 1.71E-60 | -1.69E-46 |
| MS.gene055956 | MS.gene63787 | -0.82455 | 7.60E-54 | -1.69E-46 |
| MS.gene056083 | MS.gene63787 | 0.853963 | 1.69E-61 | -1.69E-46 |
| MS.gene056374 | MS.gene63787 | 0.890461 | 9.65E-74 | -1.69E-46 |
| MS.gene056449 | MS.gene63787 | 0.802174 | 6.36E-49 | -1.69E-46 |
| MS.gene056452 | MS.gene63787 | 0.800107 | 1.68E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene63787 | 0.810014 | 1.42E-50 | -1.69E-46 |
| MS.gene056510 | MS.gene63787 | 0.830145 | 3.46E-55 | -1.69E-46 |
| MS.gene056549 | MS.gene63787 | 0.832565 | 8.79E-56 | -1.69E-46 |
| MS.gene056610 | MS.gene63787 | 0.817274 | 3.59E-52 | -1.69E-46 |
| MS.gene056657 | MS.gene63787 | 0.884224 | 2.30E-71 | -1.69E-46 |
| MS.gene056692 | MS.gene63787 | 0.843208 | 1.61E-58 | -1.69E-46 |
| MS.gene056897 | MS.gene63787 | 0.804274 | 2.34E-49 | -1.69E-46 |
| MS.gene056946 | MS.gene63787 | 0.817617 | 3.00E-52 | -1.69E-46 |
| MS.gene057011 | MS.gene63787 | 0.875921 | 2.10E-68 | -1.69E-46 |
| MS.gene057076 | MS.gene63787 | 0.81354 | 2.43E-51 | -1.69E-46 |
| MS.gene057123 | MS.gene63787 | 0.807441 | 5.05E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene63787 | 0.885768 | 6.11E-72 | -1.69E-46 |
| MS.gene057146 | MS.gene63787 | 0.847821 | 9.07E-60 | -1.69E-46 |
| MS.gene057196 | MS.gene63787 | 0.840439 | 8.69E-58 | -1.69E-46 |
| MS.gene057331 | MS.gene63787 | 0.849439 | 3.23E-60 | -1.69E-46 |
| MS.gene057332 | MS.gene63787 | 0.80611 | 9.65E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene63787 | 0.898323 | 5.94E-77 | -1.69E-46 |
| MS.gene057658 | MS.gene63787 | 0.823131 | 1.63E-53 | -1.69E-46 |
| MS.gene057831 | MS.gene63787 | 0.837765 | 4.29E-57 | -1.69E-46 |
| MS.gene058006 | MS.gene63787 | 0.813899 | 2.03E-51 | -1.69E-46 |
| MS.gene058019 | MS.gene63787 | 0.851919 | 6.49E-61 | -1.69E-46 |
| MS.gene058130 | MS.gene63787 | 0.808956 | 2.40E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene63787 | 0.803616 | 3.20E-49 | -1.69E-46 |
| MS.gene058323 | MS.gene63787 | 0.848272 | 6.81E-60 | -1.69E-46 |
| MS.gene058338 | MS.gene63787 | 0.830215 | 3.33E-55 | -1.69E-46 |
| MS.gene05837 | MS.gene63787 | 0.835706 | 1.44E-56 | -1.69E-46 |
| MS.gene058419 | MS.gene63787 | 0.901852 | 1.76E-78 | -1.69E-46 |
| MS.gene058544 | MS.gene63787 | 0.874766 | 5.23E-68 | -1.69E-46 |
| MS.gene058660 | MS.gene63787 | 0.819603 | 1.06E-52 | -1.69E-46 |
| MS.gene058723 | MS.gene63787 | 0.832807 | 7.66E-56 | -1.69E-46 |
| MS.gene058773 | MS.gene63787 | 0.854139 | 1.50E-61 | -1.69E-46 |
| MS.gene058838 | MS.gene63787 | 0.817052 | 4.03E-52 | -1.69E-46 |
| MS.gene05883 | MS.gene63787 | 0.849065 | 4.11E-60 | -1.69E-46 |
| MS.gene059022 | MS.gene63787 | 0.86664 | 2.46E-65 | -1.69E-46 |
| MS.gene059023 | MS.gene63787 | 0.860126 | 2.57E-63 | -1.69E-46 |
| MS.gene059029 | MS.gene63787 | 0.868773 | 5.09E-66 | -1.69E-46 |
| MS.gene05934 | MS.gene63787 | 0.845778 | 3.29E-59 | -1.69E-46 |
| MS.gene059536 | MS.gene63787 | 0.865753 | 4.70E-65 | -1.69E-46 |
| MS.gene059677 | MS.gene63787 | 0.887093 | 1.93E-72 | -1.69E-46 |
| MS.gene059718 | MS.gene63787 | 0.870695 | 1.20E-66 | -1.69E-46 |
| MS.gene059756 | MS.gene63787 | 0.860787 | 1.62E-63 | -1.69E-46 |
| MS.gene059801 | MS.gene63787 | 0.811854 | 5.69E-51 | -1.69E-46 |
| MS.gene059858 | MS.gene63787 | 0.809788 | 1.59E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene63787 | 0.80201 | 6.87E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene63787 | 0.867149 | 1.69E-65 | -1.69E-46 |
| MS.gene059960 | MS.gene63787 | 0.887361 | 1.53E-72 | -1.69E-46 |
| MS.gene060193 | MS.gene63787 | 0.827165 | 1.82E-54 | -1.69E-46 |
| MS.gene060675 | MS.gene63787 | 0.822417 | 2.40E-53 | -1.69E-46 |
| MS.gene060703 | MS.gene63787 | 0.823356 | 1.45E-53 | -1.69E-46 |
| MS.gene060727 | MS.gene63787 | 0.805318 | 1.41E-49 | -1.69E-46 |
| MS.gene060728 | MS.gene63787 | 0.809439 | 1.89E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene63787 | 0.82397 | 1.04E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene63787 | 0.849215 | 3.73E-60 | -1.69E-46 |
| MS.gene060804 | MS.gene63787 | 0.850591 | 1.54E-60 | -1.69E-46 |
| MS.gene060818 | MS.gene63787 | 0.823094 | 1.67E-53 | -1.69E-46 |
| MS.gene06092 | MS.gene63787 | 0.874703 | 5.49E-68 | -1.69E-46 |
| MS.gene061041 | MS.gene63787 | 0.810863 | 9.33E-51 | -1.69E-46 |
| MS.gene061134 | MS.gene63787 | 0.836902 | 7.13E-57 | -1.69E-46 |
| MS.gene061150 | MS.gene63787 | 0.898689 | 4.15E-77 | -1.69E-46 |
| MS.gene061171 | MS.gene63787 | 0.80927 | 2.06E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene63787 | 0.888654 | 4.87E-73 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63787.t1 | MTR_5g093240 | 90.123 | 81 | 5 | 1 | 1 | 81 | 1 | 78 | 6.47e-47 | 145 |
| MS.gene63787.t1 | MTR_5g093240 | 90.123 | 81 | 5 | 1 | 1 | 81 | 1 | 78 | 2.68e-46 | 143 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTTCAGTAAGTTCATTA+AGG | 0.284385 | 5.1:+74249560 | MS.gene63787:CDS |
| CGCAGCAGCAACATTATCTA+AGG | 0.361910 | 5.1:+74249473 | MS.gene63787:CDS |
| GCAGCAACAACAATGATATC+TGG | 0.364461 | 5.1:-74249595 | None:intergenic |
| GCACAACACAAAACTACTTT+GGG | 0.366214 | 5.1:+74249501 | MS.gene63787:CDS |
| ATGGATCGGCGGGTTGTGGC+AGG | 0.389363 | 5.1:-74249624 | None:intergenic |
| GAAGCAATGAAGAGGGGAGG+TGG | 0.407582 | 5.1:-74249429 | None:intergenic |
| CTATGTGGCATGGTCTTGCT+TGG | 0.417895 | 5.1:-74249529 | None:intergenic |
| AGCACAACACAAAACTACTT+TGG | 0.452055 | 5.1:+74249500 | MS.gene63787:CDS |
| CAATGATATCTGGAATTGCC+TGG | 0.462076 | 5.1:-74249585 | None:intergenic |
| CTTACTGGGAGAAAAGTAGA+TGG | 0.479013 | 5.1:-74249643 | None:intergenic |
| GTAGATGGATCGGCGGGTTG+TGG | 0.490348 | 5.1:-74249628 | None:intergenic |
| CTTAGATAATGTTGCTGCTG+CGG | 0.540896 | 5.1:-74249472 | None:intergenic |
| TGCATGAGAAGCAATGAAGA+GGG | 0.563172 | 5.1:-74249436 | None:intergenic |
| TGGATCGGCGGGTTGTGGCA+GGG | 0.568272 | 5.1:-74249623 | None:intergenic |
| AGAAAAGTAGATGGATCGGC+GGG | 0.588206 | 5.1:-74249634 | None:intergenic |
| AATGATATCTGGAATTGCCT+GGG | 0.588413 | 5.1:-74249584 | None:intergenic |
| GAAGAAAAGTGCTTACTATG+TGG | 0.588606 | 5.1:-74249544 | None:intergenic |
| TGGGAGAAAAGTAGATGGAT+CGG | 0.598368 | 5.1:-74249638 | None:intergenic |
| GCAGCAACATTATCTAAGGA+TGG | 0.606428 | 5.1:+74249477 | MS.gene63787:CDS |
| AGTAAGTTCATTAAGGACCC+AGG | 0.609846 | 5.1:+74249567 | MS.gene63787:CDS |
| AAAGTGCTTACTATGTGGCA+TGG | 0.619717 | 5.1:-74249539 | None:intergenic |
| GGGGAGGTGGAGACAAGTCA+TGG | 0.642574 | 5.1:-74249416 | None:intergenic |
| ATGCATGAGAAGCAATGAAG+AGG | 0.647708 | 5.1:-74249437 | None:intergenic |
| TGAGAAGCAATGAAGAGGGG+AGG | 0.663602 | 5.1:-74249432 | None:intergenic |
| GAGAAAAGTAGATGGATCGG+CGG | 0.670606 | 5.1:-74249635 | None:intergenic |
| GCATGAGAAGCAATGAAGAG+GGG | 0.705476 | 5.1:-74249435 | None:intergenic |
| CACAACACAAAACTACTTTG+GGG | 0.712340 | 5.1:+74249502 | MS.gene63787:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAGTATTTTTTTTTTACACT+AGG | + | chr5.1:74249718-74249737 | MS.gene63787:intron | 15.0% |
| ! | GTTCATTATGTCATATTTCA+TGG | + | chr5.1:74249688-74249707 | MS.gene63787:intron | 25.0% |
| ! | TTTCTTCAGTAAGTTCATTA+AGG | + | chr5.1:74249560-74249579 | MS.gene63787:CDS | 25.0% |
| AGCACAACACAAAACTACTT+TGG | + | chr5.1:74249500-74249519 | MS.gene63787:CDS | 35.0% | |
| CACAACACAAAACTACTTTG+GGG | + | chr5.1:74249502-74249521 | MS.gene63787:CDS | 35.0% | |
| GCACAACACAAAACTACTTT+GGG | + | chr5.1:74249501-74249520 | MS.gene63787:CDS | 35.0% | |
| ! | AATGATATCTGGAATTGCCT+GGG | - | chr5.1:74249587-74249606 | None:intergenic | 35.0% |
| !! | GAAGAAAAGTGCTTACTATG+TGG | - | chr5.1:74249547-74249566 | None:intergenic | 35.0% |
| AGAAAAGAACCGAACCTTAC+TGG | - | chr5.1:74249661-74249680 | None:intergenic | 40.0% | |
| AGTAAGTTCATTAAGGACCC+AGG | + | chr5.1:74249567-74249586 | MS.gene63787:CDS | 40.0% | |
| ATGCATGAGAAGCAATGAAG+AGG | - | chr5.1:74249440-74249459 | None:intergenic | 40.0% | |
| CTTACTGGGAGAAAAGTAGA+TGG | - | chr5.1:74249646-74249665 | None:intergenic | 40.0% | |
| CTTAGATAATGTTGCTGCTG+CGG | - | chr5.1:74249475-74249494 | None:intergenic | 40.0% | |
| GAAAAGAACCGAACCTTACT+GGG | - | chr5.1:74249660-74249679 | None:intergenic | 40.0% | |
| GCAGCAACAACAATGATATC+TGG | - | chr5.1:74249598-74249617 | None:intergenic | 40.0% | |
| GCAGCAACATTATCTAAGGA+TGG | + | chr5.1:74249477-74249496 | MS.gene63787:CDS | 40.0% | |
| TGCATGAGAAGCAATGAAGA+GGG | - | chr5.1:74249439-74249458 | None:intergenic | 40.0% | |
| TGGGAGAAAAGTAGATGGAT+CGG | - | chr5.1:74249641-74249660 | None:intergenic | 40.0% | |
| ! | AAAGTGCTTACTATGTGGCA+TGG | - | chr5.1:74249542-74249561 | None:intergenic | 40.0% |
| ! | ACTTTTCTCCCAGTAAGGTT+CGG | + | chr5.1:74249649-74249668 | MS.gene63787:intron | 40.0% |
| ! | CAATGATATCTGGAATTGCC+TGG | - | chr5.1:74249588-74249607 | None:intergenic | 40.0% |
| !! | CATCTACTTTTCTCCCAGTA+AGG | + | chr5.1:74249644-74249663 | MS.gene63787:intron | 40.0% |
| AGAAAAGTAGATGGATCGGC+GGG | - | chr5.1:74249637-74249656 | None:intergenic | 45.0% | |
| CGCAGCAGCAACATTATCTA+AGG | + | chr5.1:74249473-74249492 | MS.gene63787:CDS | 45.0% | |
| GAGAAAAGTAGATGGATCGG+CGG | - | chr5.1:74249638-74249657 | None:intergenic | 45.0% | |
| GCATGAGAAGCAATGAAGAG+GGG | - | chr5.1:74249438-74249457 | None:intergenic | 45.0% | |
| TGAGAAGCAATGAAGAGGGG+AGG | - | chr5.1:74249435-74249454 | None:intergenic | 50.0% | |
| ! | CTATGTGGCATGGTCTTGCT+TGG | - | chr5.1:74249532-74249551 | None:intergenic | 50.0% |
| GAAGCAATGAAGAGGGGAGG+TGG | - | chr5.1:74249432-74249451 | None:intergenic | 55.0% | |
| !! | GTAGATGGATCGGCGGGTTG+TGG | - | chr5.1:74249631-74249650 | None:intergenic | 60.0% |
| !! | ATGGATCGGCGGGTTGTGGC+AGG | - | chr5.1:74249627-74249646 | None:intergenic | 65.0% |
| !! | TGGATCGGCGGGTTGTGGCA+GGG | - | chr5.1:74249626-74249645 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 74249418 | 74249748 | 74249418 | ID=MS.gene63787 |
| chr5.1 | mRNA | 74249418 | 74249748 | 74249418 | ID=MS.gene63787.t1;Parent=MS.gene63787 |
| chr5.1 | exon | 74249418 | 74249660 | 74249418 | ID=MS.gene63787.t1.exon1;Parent=MS.gene63787.t1 |
| chr5.1 | CDS | 74249418 | 74249660 | 74249418 | ID=cds.MS.gene63787.t1;Parent=MS.gene63787.t1 |
| chr5.1 | exon | 74249740 | 74249748 | 74249740 | ID=MS.gene63787.t1.exon2;Parent=MS.gene63787.t1 |
| chr5.1 | CDS | 74249740 | 74249748 | 74249740 | ID=cds.MS.gene63787.t1;Parent=MS.gene63787.t1 |
| Gene Sequence |
| Protein sequence |
