AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70074


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70074.t1 MTR_4g016930 97.297 148 4 0 1 148 150 297 8.48e-104 298
MS.gene70074.t1 MTR_7g079520 41.860 129 71 2 21 148 52 177 2.27e-30 108
MS.gene70074.t1 MTR_4g088905 46.491 114 54 2 34 147 147 253 1.69e-26 103
MS.gene70074.t1 MTR_4g088905 42.991 107 54 3 40 142 382 485 1.06e-22 93.2
MS.gene70074.t1 MTR_4g088905 40.385 104 59 1 39 142 277 377 1.22e-20 87.4
MS.gene70074.t1 MTR_2g103130 35.417 96 59 1 47 142 55 147 2.04e-18 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70074.t1 AT2G38270 77.397 146 33 0 1 146 147 292 2.26e-85 252
MS.gene70074.t1 AT3G54900 44.828 116 57 2 31 142 51 163 1.17e-28 103
MS.gene70074.t1 AT4G04950 41.935 124 56 2 24 147 143 250 4.12e-24 97.1
MS.gene70074.t1 AT4G04950 41.667 96 53 1 47 142 390 482 2.35e-21 89.4
MS.gene70074.t1 AT4G04950 40.000 100 57 1 43 142 279 375 8.89e-18 79.3
MS.gene70074.t1 AT3G15660 37.374 99 59 1 44 142 62 157 2.23e-20 82.4
MS.gene70074.t1 AT3G15660 37.374 99 59 1 44 142 62 157 2.23e-20 82.4

Find 41 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACATAACGGCGCACTTC+TGG 0.276069 4.2:-85052751 None:intergenic
TTGGATGAAGATTATAACTA+TGG 0.276665 4.2:+85052837 MS.gene70074:CDS
TCTTACCCAAGTGGCGTTTC+CGG 0.280532 4.2:-85052601 None:intergenic
GCGTTTCCGGATTCATTTCC+AGG 0.302092 4.2:-85052588 None:intergenic
CGAAGATCAGCCTTCTTCTT+AGG 0.340552 4.2:-85052630 None:intergenic
GGATTCTCTCAGAGGGTAAT+AGG 0.345382 4.2:+85052771 MS.gene70074:CDS
CAAGTTGGTAAAAGAGAATA+AGG 0.354560 4.2:+85052707 MS.gene70074:CDS
GGTAATAGGCATTCTTGAAA+AGG 0.467989 4.2:+85052785 MS.gene70074:CDS
TCAGCCTTCTTCTTAGGCTG+TGG 0.482968 4.2:-85052624 None:intergenic
TTGACCTCCATGTTTGAGAA+AGG 0.485069 4.2:+85052948 MS.gene70074:CDS
AAGATTATAACTATGGATTG+AGG 0.490227 4.2:+85052844 MS.gene70074:CDS
TTATGAGAGTGTAGATGTAT+TGG 0.491403 4.2:+85052818 MS.gene70074:CDS
GTTCCACCTGGAAATGAATC+CGG 0.492241 4.2:+85052582 MS.gene70074:CDS
CACTTCACCTTTCTCAAACA+TGG 0.492756 4.2:-85052955 None:intergenic
TCCAGAAGTGCGCCGTTATG+TGG 0.500421 4.2:+85052750 MS.gene70074:CDS
AGATTATAACTATGGATTGA+GGG 0.506926 4.2:+85052845 MS.gene70074:CDS
TTGAATGGTGAACTTGTTGG+TGG 0.513486 4.2:+85052915 MS.gene70074:CDS
ATGAATCCGGAAACGCCACT+TGG 0.525120 4.2:+85052595 MS.gene70074:CDS
TAAGGTAGTTGCATTCATCA+AGG 0.556799 4.2:+85052725 MS.gene70074:CDS
TGAAGAGCTCGCGGACAAGT+TGG 0.558657 4.2:+85052692 MS.gene70074:CDS
TTTGAGAAAGGTGAAGTGGC+TGG 0.570458 4.2:+85052960 MS.gene70074:CDS
GTTAGTTGCACGTGACGACC+AGG 0.575913 4.2:-85052660 None:intergenic
AAGACCACAGCCTAAGAAGA+AGG 0.577244 4.2:+85052620 MS.gene70074:CDS
CGCGAGCTCTTCAAGAGGGA+CGG 0.580487 4.2:-85052682 None:intergenic
AAGAACACATAAAGATCAGT+GGG 0.590962 4.2:+85052556 MS.gene70074:CDS
ATCAGTGGGAAAGTTCCACC+TGG 0.600855 4.2:+85052570 MS.gene70074:CDS
CGTTATGTGGATTCTCTCAG+AGG 0.601281 4.2:+85052763 MS.gene70074:CDS
GAAGAACACATAAAGATCAG+TGG 0.602390 4.2:+85052555 MS.gene70074:CDS
TTGTCCGCGAGCTCTTCAAG+AGG 0.606513 4.2:-85052687 None:intergenic
ATAGGCATTCTTGAAAAGGA+AGG 0.610176 4.2:+85052789 MS.gene70074:CDS
CTCTGAGAGAATCCACATAA+CGG 0.611300 4.2:-85052762 None:intergenic
TGAATCCGGAAACGCCACTT+GGG 0.613599 4.2:+85052596 MS.gene70074:CDS
AAGGTAGTTGCATTCATCAA+GGG 0.614128 4.2:+85052726 MS.gene70074:CDS
TGTCCGCGAGCTCTTCAAGA+GGG 0.639718 4.2:-85052686 None:intergenic
GCTGATCTTCGATTAACACC+TGG 0.649446 4.2:+85052642 MS.gene70074:CDS
CATGTTTGAGAAAGGTGAAG+TGG 0.660920 4.2:+85052956 MS.gene70074:CDS
GTTATGTGGATTCTCTCAGA+GGG 0.661671 4.2:+85052764 MS.gene70074:CDS
CGTCCCTCTTGAAGAGCTCG+CGG 0.666440 4.2:+85052683 MS.gene70074:CDS
TTTCCGGATTCATTTCCAGG+TGG 0.667945 4.2:-85052585 None:intergenic
AGGCTGTGGTCTTACCCAAG+TGG 0.670747 4.2:-85052610 None:intergenic
TTCACCTTTCTCAAACATGG+AGG 0.696862 4.2:-85052952 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGGATGAAGATTATAACTA+TGG + chr4.2:85052837-85052856 MS.gene70074:CDS 25.0%
!! AAGATTATAACTATGGATTG+AGG + chr4.2:85052844-85052863 MS.gene70074:CDS 25.0%
!! AGATTATAACTATGGATTGA+GGG + chr4.2:85052845-85052864 MS.gene70074:CDS 25.0%
AAAAATATCTGAGGGAATGT+AGG - chr4.2:85052897-85052916 None:intergenic 30.0%
AAGAACACATAAAGATCAGT+GGG + chr4.2:85052556-85052575 MS.gene70074:CDS 30.0%
CACCATTCAAAAATATCTGA+GGG - chr4.2:85052905-85052924 None:intergenic 30.0%
TCACCATTCAAAAATATCTG+AGG - chr4.2:85052906-85052925 None:intergenic 30.0%
! CACTGAAAAAGTATAGCAAT+TGG + chr4.2:85052871-85052890 MS.gene70074:CDS 30.0%
! TTATGAGAGTGTAGATGTAT+TGG + chr4.2:85052818-85052837 MS.gene70074:CDS 30.0%
! TTTTTGAATGGTGAACTTGT+TGG + chr4.2:85052912-85052931 MS.gene70074:CDS 30.0%
!! CAAGTTGGTAAAAGAGAATA+AGG + chr4.2:85052707-85052726 MS.gene70074:CDS 30.0%
!! TTCCCTCAGATATTTTTGAA+TGG + chr4.2:85052900-85052919 MS.gene70074:CDS 30.0%
AAGGTAGTTGCATTCATCAA+GGG + chr4.2:85052726-85052745 MS.gene70074:CDS 35.0%
ATAGGCATTCTTGAAAAGGA+AGG + chr4.2:85052789-85052808 MS.gene70074:CDS 35.0%
GAAGAACACATAAAGATCAG+TGG + chr4.2:85052555-85052574 MS.gene70074:CDS 35.0%
GGTAATAGGCATTCTTGAAA+AGG + chr4.2:85052785-85052804 MS.gene70074:CDS 35.0%
TAAGGTAGTTGCATTCATCA+AGG + chr4.2:85052725-85052744 MS.gene70074:CDS 35.0%
CACTTCACCTTTCTCAAACA+TGG - chr4.2:85052958-85052977 None:intergenic 40.0%
CATGTTTGAGAAAGGTGAAG+TGG + chr4.2:85052956-85052975 MS.gene70074:CDS 40.0%
GTTATGTGGATTCTCTCAGA+GGG + chr4.2:85052764-85052783 MS.gene70074:CDS 40.0%
TTCACCTTTCTCAAACATGG+AGG - chr4.2:85052955-85052974 None:intergenic 40.0%
TTGACCTCCATGTTTGAGAA+AGG + chr4.2:85052948-85052967 MS.gene70074:CDS 40.0%
! CTCTGAGAGAATCCACATAA+CGG - chr4.2:85052765-85052784 None:intergenic 40.0%
!! TTGAATGGTGAACTTGTTGG+TGG + chr4.2:85052915-85052934 MS.gene70074:CDS 40.0%
AAGACCACAGCCTAAGAAGA+AGG + chr4.2:85052620-85052639 MS.gene70074:CDS 45.0%
CGAAGATCAGCCTTCTTCTT+AGG - chr4.2:85052633-85052652 None:intergenic 45.0%
CGTTATGTGGATTCTCTCAG+AGG + chr4.2:85052763-85052782 MS.gene70074:CDS 45.0%
GCTGATCTTCGATTAACACC+TGG + chr4.2:85052642-85052661 MS.gene70074:CDS 45.0%
GGATTCTCTCAGAGGGTAAT+AGG + chr4.2:85052771-85052790 MS.gene70074:CDS 45.0%
GTTCCACCTGGAAATGAATC+CGG + chr4.2:85052582-85052601 MS.gene70074:CDS 45.0%
TTTCCGGATTCATTTCCAGG+TGG - chr4.2:85052588-85052607 None:intergenic 45.0%
TTTGAGAAAGGTGAAGTGGC+TGG + chr4.2:85052960-85052979 MS.gene70074:CDS 45.0%
ATCAGTGGGAAAGTTCCACC+TGG + chr4.2:85052570-85052589 MS.gene70074:CDS 50.0%
ATGAATCCGGAAACGCCACT+TGG + chr4.2:85052595-85052614 MS.gene70074:CDS 50.0%
GCGTTTCCGGATTCATTTCC+AGG - chr4.2:85052591-85052610 None:intergenic 50.0%
TCAGCCTTCTTCTTAGGCTG+TGG - chr4.2:85052627-85052646 None:intergenic 50.0%
TCTTACCCAAGTGGCGTTTC+CGG - chr4.2:85052604-85052623 None:intergenic 50.0%
TGAATCCGGAAACGCCACTT+GGG + chr4.2:85052596-85052615 MS.gene70074:CDS 50.0%
GTTAGTTGCACGTGACGACC+AGG - chr4.2:85052663-85052682 None:intergenic 55.0%
TCCACATAACGGCGCACTTC+TGG - chr4.2:85052754-85052773 None:intergenic 55.0%
TGAAGAGCTCGCGGACAAGT+TGG + chr4.2:85052692-85052711 MS.gene70074:CDS 55.0%
TGTCCGCGAGCTCTTCAAGA+GGG - chr4.2:85052689-85052708 None:intergenic 55.0%
TTGTCCGCGAGCTCTTCAAG+AGG - chr4.2:85052690-85052709 None:intergenic 55.0%
! AGGCTGTGGTCTTACCCAAG+TGG - chr4.2:85052613-85052632 None:intergenic 55.0%
! TCCAGAAGTGCGCCGTTATG+TGG + chr4.2:85052750-85052769 MS.gene70074:CDS 55.0%
CGCGAGCTCTTCAAGAGGGA+CGG - chr4.2:85052685-85052704 None:intergenic 60.0%
CGTCCCTCTTGAAGAGCTCG+CGG + chr4.2:85052683-85052702 MS.gene70074:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 85052552 85052998 85052552 ID=MS.gene70074
chr4.2 mRNA 85052552 85052998 85052552 ID=MS.gene70074.t1;Parent=MS.gene70074
chr4.2 exon 85052552 85052998 85052552 ID=MS.gene70074.t1.exon1;Parent=MS.gene70074.t1
chr4.2 CDS 85052552 85052998 85052552 ID=cds.MS.gene70074.t1;Parent=MS.gene70074.t1
Gene Sequence

>MS.gene70074

ATGGAAGAACACATAAAGATCAGTGGGAAAGTTCCACCTGGAAATGAATCCGGAAACGCCACTTGGGTAAGACCACAGCCTAAGAAGAAGGCTGATCTTCGATTAACACCTGGTCGTCACGTGCAACTAACCGTCCCTCTTGAAGAGCTCGCGGACAAGTTGGTAAAAGAGAATAAGGTAGTTGCATTCATCAAGGGATCCAGAAGTGCGCCGTTATGTGGATTCTCTCAGAGGGTAATAGGCATTCTTGAAAAGGAAGGAGTAGATTATGAGAGTGTAGATGTATTGGATGAAGATTATAACTATGGATTGAGGGAGACACTGAAAAAGTATAGCAATTGGCCTACATTCCCTCAGATATTTTTGAATGGTGAACTTGTTGGTGGTTGTGATATATTGACCTCCATGTTTGAGAAAGGTGAAGTGGCTGGTTTGTTCAAAAAGTAA

Protein sequence

>MS.gene70074.t1

MEEHIKISGKVPPGNESGNATWVRPQPKKKADLRLTPGRHVQLTVPLEELADKLVKENKVVAFIKGSRSAPLCGFSQRVIGILEKEGVDYESVDVLDEDYNYGLRETLKKYSNWPTFPQIFLNGELVGGCDILTSMFEKGEVAGLFKK