AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037331.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037331.01.T01 AT3G46610 67.045 88 28 1 1 88 578 664 3.89e-36 129
MsG0780037331.01.T01 AT3G46610 67.045 88 28 1 1 88 578 664 3.89e-36 129

Find 17 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGTGCCACTATTGATAT+AGG 0.306782 7:+23674529 MsG0780037331.01.T01:CDS
TTGCAATCCTCTAAGGTTCA+TGG 0.357840 7:+23674511 MsG0780037331.01.T01:CDS
ATTTCATAAGTTACCTCATT+GGG 0.395807 7:-23674379 None:intergenic
TTGGCTTTCCATCGTTCGCA+AGG 0.460104 7:-23674414 None:intergenic
CATTTCATAAGTTACCTCAT+TGG 0.465745 7:-23674380 None:intergenic
AGTGGCACCATGAACCTTAG+AGG 0.485274 7:-23674518 None:intergenic
TGAGTAGTGCAGCGTATGAA+TGG 0.506016 7:+23674326 MsG0780037331.01.T01:CDS
TGGCTTTCCATCGTTCGCAA+GGG 0.530441 7:-23674413 None:intergenic
TATAACTCATACGCAAGCCT+TGG 0.532210 7:-23674433 None:intergenic
AGAGCTCAGAATGAGGGCCT+CGG 0.532473 7:+23674460 MsG0780037331.01.T01:CDS
TATTTGAGAGCTCAGAATGA+GGG 0.544302 7:+23674454 MsG0780037331.01.T01:CDS
ATTGAGGCCCTTGCGAACGA+TGG 0.551286 7:+23674406 MsG0780037331.01.T01:CDS
ATATTTGAGAGCTCAGAATG+AGG 0.579174 7:+23674453 MsG0780037331.01.T01:CDS
AACTTATGAAATGCTGATTG+AGG 0.587767 7:+23674390 MsG0780037331.01.T01:CDS
TCAGAGCATCACTCCCAATG+AGG 0.654791 7:+23674366 MsG0780037331.01.T01:CDS
TTGCGAACGATGGAAAGCCA+AGG 0.659971 7:+23674416 MsG0780037331.01.T01:CDS
AAGCTTTGAAAGATAGACCG+AGG 0.705314 7:-23674477 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAACCTATATCAATAG+TGG - Chr7:23674539-23674558 None:intergenic 20.0%
!!! ACTATTGATATAGGTTTTTT+AGG + Chr7:23674538-23674557 MsG0780037331.01.T01:CDS 20.0%
! ATTTCATAAGTTACCTCATT+GGG - Chr7:23674382-23674401 None:intergenic 25.0%
CATTTCATAAGTTACCTCAT+TGG - Chr7:23674383-23674402 None:intergenic 30.0%
! AACTTATGAAATGCTGATTG+AGG + Chr7:23674390-23674409 MsG0780037331.01.T01:CDS 30.0%
!! ACTTTAGTCTTTTTATCTGC+GGG - Chr7:23674571-23674590 None:intergenic 30.0%
!! CACTTTAGTCTTTTTATCTG+CGG - Chr7:23674572-23674591 None:intergenic 30.0%
ATATTTGAGAGCTCAGAATG+AGG + Chr7:23674453-23674472 MsG0780037331.01.T01:CDS 35.0%
TATTTGAGAGCTCAGAATGA+GGG + Chr7:23674454-23674473 MsG0780037331.01.T01:CDS 35.0%
TATAACTCATACGCAAGCCT+TGG - Chr7:23674436-23674455 None:intergenic 40.0%
! TGCAGTTTTGCAATCCTCTA+AGG + Chr7:23674504-23674523 MsG0780037331.01.T01:CDS 40.0%
!! AAGCTTTGAAAGATAGACCG+AGG - Chr7:23674480-23674499 None:intergenic 40.0%
!! CATGGTGCCACTATTGATAT+AGG + Chr7:23674529-23674548 MsG0780037331.01.T01:CDS 40.0%
!! TTAGTCTTTTTATCTGCGGG+AGG - Chr7:23674568-23674587 None:intergenic 40.0%
!! TTGCAATCCTCTAAGGTTCA+TGG + Chr7:23674511-23674530 MsG0780037331.01.T01:CDS 40.0%
TGAGTAGTGCAGCGTATGAA+TGG + Chr7:23674326-23674345 MsG0780037331.01.T01:CDS 45.0%
AGTGGCACCATGAACCTTAG+AGG - Chr7:23674521-23674540 None:intergenic 50.0%
TGGCTTTCCATCGTTCGCAA+GGG - Chr7:23674416-23674435 None:intergenic 50.0%
TTGCGAACGATGGAAAGCCA+AGG + Chr7:23674416-23674435 MsG0780037331.01.T01:CDS 50.0%
! GTCTTTTTATCTGCGGGAGG+AGG - Chr7:23674565-23674584 None:intergenic 50.0%
! TCAGAGCATCACTCCCAATG+AGG + Chr7:23674366-23674385 MsG0780037331.01.T01:CDS 50.0%
! TTGGCTTTCCATCGTTCGCA+AGG - Chr7:23674417-23674436 None:intergenic 50.0%
AGAGCTCAGAATGAGGGCCT+CGG + Chr7:23674460-23674479 MsG0780037331.01.T01:CDS 55.0%
ATTGAGGCCCTTGCGAACGA+TGG + Chr7:23674406-23674425 MsG0780037331.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 23674325 23674615 23674325 ID=MsG0780037331.01;Name=MsG0780037331.01
Chr7 mRNA 23674325 23674615 23674325 ID=MsG0780037331.01.T01;Parent=MsG0780037331.01;Name=MsG0780037331.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|96
Chr7 exon 23674325 23674615 23674325 ID=MsG0780037331.01.T01:exon:6477;Parent=MsG0780037331.01.T01
Chr7 CDS 23674325 23674615 23674325 ID=MsG0780037331.01.T01:cds;Parent=MsG0780037331.01.T01
Gene Sequence

>MsG0780037331.01.T01

ATGAGTAGTGCAGCGTATGAATGGTTTCACAGAATGAAAGTTCAGAGCATCACTCCCAATGAGGTAACTTATGAAATGCTGATTGAGGCCCTTGCGAACGATGGAAAGCCAAGGCTTGCGTATGAGTTATATTTGAGAGCTCAGAATGAGGGCCTCGGTCTATCTTTCAAAGCTTATGATGCAGTTTTGCAATCCTCTAAGGTTCATGGTGCCACTATTGATATAGGTTTTTTAGGACCTCCTCCCGCAGATAAAAAGACTAAAGTGAAATTTAGAAAAAATATCGACTGA

Protein sequence

>MsG0780037331.01.T01

MSSAAYEWFHRMKVQSITPNEVTYEMLIEALANDGKPRLAYELYLRAQNEGLGLSFKAYDAVLQSSKVHGATIDIGFLGPPPADKKTKVKFRKNID*