AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036800.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036800.01.T02 MTR_7g019160 100.000 37 0 0 1 37 26 62 1.01e-19 73.2
MsG0780036800.01.T02 MTR_6g016890 77.143 35 8 0 1 35 27 61 3.03e-13 57.0
MsG0780036800.01.T01 MTR_7g019160 96.774 62 2 0 1 62 1 62 2.26e-36 116
MsG0780036800.01.T01 MTR_6g016890 65.000 60 21 0 1 60 2 61 3.24e-21 78.2
MsG0780036800.01.T01 MTR_5g005330 61.290 62 18 3 3 58 5 66 1.82e-13 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 7 sgRNAs with CRISPR-Local

Find 7 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCTCTTCAATAAGCTTTC+TGG 0.211273 7:+14120940 None:intergenic
CTCTTCTCCATCTTATCATA+TGG 0.344037 7:+14120998 None:intergenic
CAAGAAAGGGATATGGGTCT+TGG 0.416355 7:-14121073 None:intergenic
GGATATGGGTCTTGGAAGAC+TGG 0.493445 7:-14121065 None:intergenic
GAAGAAGCCATATGATAAGA+TGG 0.518528 7:-14121005 MsG0780036800.01.T01:CDS
GAAGAGTGAGAGTATGAGAG+TGG 0.529189 7:-14120981 MsG0780036800.01.T01:CDS
ATGGGTCTTGGAAGACTGGT+AGG 0.573515 7:-14121061 MsG0780036800.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTCAAAGACTTTATTTT+TGG + Chr7:14120931-14120950 None:intergenic 15.0%
!! AAGTTGAAAGATTTTGTCTT+TGG + Chr7:14121060-14121079 None:intergenic 25.0%
AGTCTCTTCAATAAGCTTTC+TGG + Chr7:14121019-14121038 None:intergenic 35.0%
CTCTTCTCCATCTTATCATA+TGG + Chr7:14120961-14120980 None:intergenic 35.0%
GAAGAAGCCATATGATAAGA+TGG - Chr7:14120951-14120970 MsG0780036800.01.T01:CDS 35.0%
! GAAGAGTGAGAGTATGAGAG+TGG - Chr7:14120975-14120994 MsG0780036800.01.T01:CDS 45.0%
ATGGGTCTTGGAAGACTGGT+AGG - Chr7:14120895-14120914 MsG0780036800.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 14120895 14121083 14120895 ID=MsG0780036800.01;Name=MsG0780036800.01
Chr7 mRNA 14120895 14121083 14120895 ID=MsG0780036800.01.T01;Parent=MsG0780036800.01;Name=MsG0780036800.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|62
Chr7 exon 14120895 14121083 14120895 ID=MsG0780036800.01.T01:exon:2927;Parent=MsG0780036800.01.T01
Chr7 CDS 14120895 14121083 14120895 ID=MsG0780036800.01.T01:cds;Parent=MsG0780036800.01.T01
Chr7 mRNA 14120895 14121008 14120895 ID=MsG0780036800.01.T02;Parent=MsG0780036800.01;Name=MsG0780036800.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|37
Chr7 exon 14120895 14121008 14120895 ID=MsG0780036800.01.T02:exon:2928;Parent=MsG0780036800.01.T02
Chr7 CDS 14120895 14121008 14120895 ID=MsG0780036800.01.T02:cds;Parent=MsG0780036800.01.T02
Gene Sequence

>MsG0780036800.01.T02

ATGGAGAAGAGTGAGAGTATGAGAGTGGAGATAAAGAGCAGAAAAGCCAGAAAGCTTATTGAAGAGACTCTCAAAATTGCTGATTCACCAAAGACAAAATCTTTCAACTTCTGA

>MsG0780036800.01.T01

ATGGGTCTTGGAAGACTGGTAGGAACACTAAATCCAAAAATAAAGTCTTTGAAATTGAAGAAGCCATATGATAAGATGGAGAAGAGTGAGAGTATGAGAGTGGAGATAAAGAGCAGAAAAGCCAGAAAGCTTATTGAAGAGACTCTCAAAATTGCTGATTCACCAAAGACAAAATCTTTCAACTTCTGA

Protein sequence

>MsG0780036800.01.T02

MEKSESMRVEIKSRKARKLIEETLKIADSPKTKSFNF*

>MsG0780036800.01.T01

MGLGRLVGTLNPKIKSLKLKKPYDKMEKSESMRVEIKSRKARKLIEETLKIADSPKTKSFNF*