AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0061380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0061380 MtrunA17_Chr1g0161491 93.370 181 12 0 6 186 10 190 7.54e-115 352
Msa0061380 MtrunA17_Chr1g0161521 75.419 179 41 2 11 186 1 179 2.80e-84 270
Msa0061380 MtrunA17_Chr1g0161511 69.000 200 56 4 11 204 1 200 3.30e-83 267
Msa0061380 MtrunA17_Chr1g0161471 70.950 179 49 2 11 186 1 179 7.98e-80 258
Msa0061380 MtrunA17_Chr1g0161451 34.104 173 112 2 14 185 2 173 2.19e-26 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0061380 AT1G14790.1 45.641 195 97 3 15 201 3 196 3.02e-52 180

Find 39 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACGATGTTAAACTGTATTT+TGG 0.159141 1_2:-19457544 Msa0061380:CDS
GTGGATGCTAGTGTAGATTT+TGG 0.172622 1_2:-19457481 Msa0061380:CDS
AGCTTTCATATGAGAATATT+TGG 0.191630 1_2:-19457399 Msa0061380:CDS
GGAACTCCTATTTAAAAGTT+AGG 0.218706 1_2:-19457618 Msa0061380:CDS
TCCAAAGCCAAGGGCATTCT+TGG 0.267359 1_2:-19457575 Msa0061380:CDS
TGCAAAGTATCTATTGATTC+AGG 0.268018 1_2:-19457344 Msa0061380:CDS
AGGAAAGATTCTCTATATTA+TGG 0.268444 1_2:-19457507 Msa0061380:CDS
TCCAAGAATGCCCTTGGCTT+TGG 0.307615 1_2:+19457574 None:intergenic
ACCATTGTTCATAATTCATA+TGG 0.333704 1_2:+19457194 None:intergenic
AGTAAAACAATTGAGTTGTA+TGG 0.346819 1_2:-19457835 Msa0061380:CDS
TGAATAATTGCAAATGCTCT+TGG 0.351532 1_2:+19457706 None:intergenic
GCAGAACTAAATTGCGGTTC+GGG 0.394506 1_2:-19457639 Msa0061380:CDS
CTATTTAAAAGTTAGGGAAA+TGG 0.416320 1_2:-19457611 Msa0061380:CDS
AGCAGAACTAAATTGCGGTT+CGG 0.428037 1_2:-19457640 Msa0061380:CDS
TGAGACACGTTCACAAAAGA+AGG 0.445945 1_2:+19457808 None:intergenic
GAACTCCTATTTAAAAGTTA+GGG 0.447970 1_2:-19457617 Msa0061380:CDS
TTGGGATTGGTATGAGAAAG+TGG 0.485612 1_2:-19457462 Msa0061380:CDS
TGTTTAAAATATTTAATGAA+AGG 0.487953 1_2:+19457308 None:intergenic
CAATTTAGTTCTGCTAGGTA+AGG 0.518592 1_2:+19457648 None:intergenic
AGCAACCACACTTCCTTCAC+CGG 0.525082 1_2:+19457759 None:intergenic
GATTGAGCTGCATCAACCAC+AGG 0.538892 1_2:-19457374 Msa0061380:CDS
AAACTGTCCAAGAATGCCCT+TGG 0.565377 1_2:+19457568 None:intergenic
GTAGAGCATTATACCGGTGA+AGG 0.569466 1_2:-19457772 Msa0061380:CDS
ATTCTCTATATTATGGAAGG+TGG 0.569959 1_2:-19457500 Msa0061380:CDS
ATATTTGTAGAGCATTATAC+CGG 0.575930 1_2:-19457778 Msa0061380:CDS
AAGATTCTCTATATTATGGA+AGG 0.592994 1_2:-19457503 Msa0061380:CDS
AAAACATGCTACATTCATGA+TGG 0.594459 1_2:-19457671 Msa0061380:CDS
GTTGCTATGAAGATTAGAGT+AGG 0.605906 1_2:-19457742 Msa0061380:CDS
GAGATATTGATCCAAAGCCA+AGG 0.607190 1_2:-19457585 Msa0061380:CDS
TACCTAGCAGAACTAAATTG+CGG 0.623189 1_2:-19457645 Msa0061380:CDS
GAGACACGTTCACAAAAGAA+GGG 0.638551 1_2:+19457809 None:intergenic
AGATATTGATCCAAAGCCAA+GGG 0.640291 1_2:-19457584 Msa0061380:CDS
ATATGAATTATGAACAATGG+TGG 0.643067 1_2:-19457192 Msa0061380:three_prime_UTR
ATGAAGATTAGAGTAGGCAA+AGG 0.643746 1_2:-19457736 Msa0061380:CDS
AACCGCAATTTAGTTCTGCT+AGG 0.654522 1_2:+19457643 None:intergenic
TCCATATGAATTATGAACAA+TGG 0.662687 1_2:-19457195 Msa0061380:three_prime_UTR
TTATACCGGTGAAGGAAGTG+TGG 0.673698 1_2:-19457764 Msa0061380:CDS
ATTGAGCTGCATCAACCACA+GGG 0.682058 1_2:-19457373 Msa0061380:CDS
GGAAAGATTCAAGAATGACA+TGG 0.688166 1_2:-19457106 Msa0061380:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAAAATTGAAAAAAA+AGG + chr1_2:19457210-19457229 None:intergenic 10.0%
!!! TGTTTAAAATATTTAATGAA+AGG + chr1_2:19457812-19457831 None:intergenic 10.0%
!! AGAAAAAAAATTGAAAAAAA+AGG + chr1_2:19457210-19457229 None:intergenic 10.0%
!!! TGTTTAAAATATTTAATGAA+AGG + chr1_2:19457812-19457831 None:intergenic 10.0%
!!! GAATAATAAATGATTTTCTA+TGG + chr1_2:19457131-19457150 None:intergenic 15.0%
!!! GAATAATAAATGATTTTCTA+TGG + chr1_2:19457131-19457150 None:intergenic 15.0%
!!! ATATCAAGAATGATTTTATC+AGG - chr1_2:19457990-19458009 Msa0061380:intron 20.0%
!!! ATATCAAGAATGATTTTATC+AGG - chr1_2:19457990-19458009 Msa0061380:intron 20.0%
!! AGTAAAACAATTGAGTTGTA+TGG - chr1_2:19457282-19457301 Msa0061380:CDS 25.0%
!! ATATTTGTAGAGCATTATAC+CGG - chr1_2:19457339-19457358 Msa0061380:CDS 25.0%
! GAACTCCTATTTAAAAGTTA+GGG - chr1_2:19457500-19457519 Msa0061380:CDS 25.0%
!! CATTTCCCTAACTTTTAAAT+AGG + chr1_2:19457508-19457527 None:intergenic 25.0%
! CTATTTAAAAGTTAGGGAAA+TGG - chr1_2:19457506-19457525 Msa0061380:CDS 25.0%
! AGGAAAGATTCTCTATATTA+TGG - chr1_2:19457610-19457629 Msa0061380:CDS 25.0%
! AAGATTCTCTATATTATGGA+AGG - chr1_2:19457614-19457633 Msa0061380:CDS 25.0%
!!! GTTTGAACTTCTTATTTTCA+TGG + chr1_2:19457695-19457714 None:intergenic 25.0%
! AGCTTTCATATGAGAATATT+TGG - chr1_2:19457718-19457737 Msa0061380:CDS 25.0%
! TCCATATGAATTATGAACAA+TGG - chr1_2:19457922-19457941 Msa0061380:intron 25.0%
! ACCATTGTTCATAATTCATA+TGG + chr1_2:19457926-19457945 None:intergenic 25.0%
! ATATGAATTATGAACAATGG+TGG - chr1_2:19457925-19457944 Msa0061380:intron 25.0%
!! AGTAAAACAATTGAGTTGTA+TGG - chr1_2:19457282-19457301 Msa0061380:CDS 25.0%
!! ATATTTGTAGAGCATTATAC+CGG - chr1_2:19457339-19457358 Msa0061380:CDS 25.0%
! GAACTCCTATTTAAAAGTTA+GGG - chr1_2:19457500-19457519 Msa0061380:CDS 25.0%
!! CATTTCCCTAACTTTTAAAT+AGG + chr1_2:19457508-19457527 None:intergenic 25.0%
! CTATTTAAAAGTTAGGGAAA+TGG - chr1_2:19457506-19457525 Msa0061380:CDS 25.0%
! AGGAAAGATTCTCTATATTA+TGG - chr1_2:19457610-19457629 Msa0061380:CDS 25.0%
! AAGATTCTCTATATTATGGA+AGG - chr1_2:19457614-19457633 Msa0061380:CDS 25.0%
!!! GTTTGAACTTCTTATTTTCA+TGG + chr1_2:19457695-19457714 None:intergenic 25.0%
! AGCTTTCATATGAGAATATT+TGG - chr1_2:19457718-19457737 Msa0061380:CDS 25.0%
! TCCATATGAATTATGAACAA+TGG - chr1_2:19457922-19457941 Msa0061380:intron 25.0%
! ACCATTGTTCATAATTCATA+TGG + chr1_2:19457926-19457945 None:intergenic 25.0%
! ATATGAATTATGAACAATGG+TGG - chr1_2:19457925-19457944 Msa0061380:intron 25.0%
!!! CTTTTTTGACTTTCCTGAAT+AGG + chr1_2:19457241-19457260 None:intergenic 30.0%
! AAAAAAGTTAGCACTGAAGA+TGG - chr1_2:19457254-19457273 Msa0061380:three_prime_UTR 30.0%
! AAAAAGTTAGCACTGAAGAT+GGG - chr1_2:19457255-19457274 Msa0061380:three_prime_UTR 30.0%
TGAATAATTGCAAATGCTCT+TGG + chr1_2:19457414-19457433 None:intergenic 30.0%
AAAACATGCTACATTCATGA+TGG - chr1_2:19457446-19457465 Msa0061380:CDS 30.0%
GGAACTCCTATTTAAAAGTT+AGG - chr1_2:19457499-19457518 Msa0061380:CDS 30.0%
! GACGATGTTAAACTGTATTT+TGG - chr1_2:19457573-19457592 Msa0061380:CDS 30.0%
ATTCTCTATATTATGGAAGG+TGG - chr1_2:19457617-19457636 Msa0061380:CDS 30.0%
!! TGCAAAGTATCTATTGATTC+AGG - chr1_2:19457773-19457792 Msa0061380:CDS 30.0%
!!! CTTTTTTGACTTTCCTGAAT+AGG + chr1_2:19457241-19457260 None:intergenic 30.0%
! AAAAAAGTTAGCACTGAAGA+TGG - chr1_2:19457254-19457273 Msa0061380:three_prime_UTR 30.0%
! AAAAAGTTAGCACTGAAGAT+GGG - chr1_2:19457255-19457274 Msa0061380:three_prime_UTR 30.0%
TGAATAATTGCAAATGCTCT+TGG + chr1_2:19457414-19457433 None:intergenic 30.0%
AAAACATGCTACATTCATGA+TGG - chr1_2:19457446-19457465 Msa0061380:CDS 30.0%
GGAACTCCTATTTAAAAGTT+AGG - chr1_2:19457499-19457518 Msa0061380:CDS 30.0%
! GACGATGTTAAACTGTATTT+TGG - chr1_2:19457573-19457592 Msa0061380:CDS 30.0%
ATTCTCTATATTATGGAAGG+TGG - chr1_2:19457617-19457636 Msa0061380:CDS 30.0%
!! TGCAAAGTATCTATTGATTC+AGG - chr1_2:19457773-19457792 Msa0061380:CDS 30.0%
TTTCTTCTGCTATCCTATTC+AGG - chr1_2:19457225-19457244 Msa0061380:three_prime_UTR 35.0%
GTTGCTATGAAGATTAGAGT+AGG - chr1_2:19457375-19457394 Msa0061380:CDS 35.0%
ATGAAGATTAGAGTAGGCAA+AGG - chr1_2:19457381-19457400 Msa0061380:CDS 35.0%
!! CAATTTAGTTCTGCTAGGTA+AGG + chr1_2:19457472-19457491 None:intergenic 35.0%
TACCTAGCAGAACTAAATTG+CGG - chr1_2:19457472-19457491 Msa0061380:CDS 35.0%
AGATATTGATCCAAAGCCAA+GGG - chr1_2:19457533-19457552 Msa0061380:CDS 35.0%
! TGGATGCTAGTGTAGATTTT+GGG - chr1_2:19457637-19457656 Msa0061380:CDS 35.0%
GGAAAGATTCAAGAATGACA+TGG - chr1_2:19458011-19458030 Msa0061380:intron 35.0%
TTTCTTCTGCTATCCTATTC+AGG - chr1_2:19457225-19457244 Msa0061380:three_prime_UTR 35.0%
GTTGCTATGAAGATTAGAGT+AGG - chr1_2:19457375-19457394 Msa0061380:CDS 35.0%
ATGAAGATTAGAGTAGGCAA+AGG - chr1_2:19457381-19457400 Msa0061380:CDS 35.0%
!! CAATTTAGTTCTGCTAGGTA+AGG + chr1_2:19457472-19457491 None:intergenic 35.0%
TACCTAGCAGAACTAAATTG+CGG - chr1_2:19457472-19457491 Msa0061380:CDS 35.0%
AGATATTGATCCAAAGCCAA+GGG - chr1_2:19457533-19457552 Msa0061380:CDS 35.0%
! TGGATGCTAGTGTAGATTTT+GGG - chr1_2:19457637-19457656 Msa0061380:CDS 35.0%
GGAAAGATTCAAGAATGACA+TGG - chr1_2:19458011-19458030 Msa0061380:intron 35.0%
GAGACACGTTCACAAAAGAA+GGG + chr1_2:19457311-19457330 None:intergenic 40.0%
TGAGACACGTTCACAAAAGA+AGG + chr1_2:19457312-19457331 None:intergenic 40.0%
!! AACCGCAATTTAGTTCTGCT+AGG + chr1_2:19457477-19457496 None:intergenic 40.0%
AGCAGAACTAAATTGCGGTT+CGG - chr1_2:19457477-19457496 Msa0061380:CDS 40.0%
GAGATATTGATCCAAAGCCA+AGG - chr1_2:19457532-19457551 Msa0061380:CDS 40.0%
!! TTTTGGCTGTCAGATCTCAA+AGG - chr1_2:19457590-19457609 Msa0061380:CDS 40.0%
! GTGGATGCTAGTGTAGATTT+TGG - chr1_2:19457636-19457655 Msa0061380:CDS 40.0%
!!! GCTAGTGTAGATTTTGGGAT+TGG - chr1_2:19457642-19457661 Msa0061380:CDS 40.0%
! TTGGGATTGGTATGAGAAAG+TGG - chr1_2:19457655-19457674 Msa0061380:CDS 40.0%
! TACTTTGCAGTTTTACCCTG+TGG + chr1_2:19457762-19457781 None:intergenic 40.0%
GAGACACGTTCACAAAAGAA+GGG + chr1_2:19457311-19457330 None:intergenic 40.0%
TGAGACACGTTCACAAAAGA+AGG + chr1_2:19457312-19457331 None:intergenic 40.0%
!! AACCGCAATTTAGTTCTGCT+AGG + chr1_2:19457477-19457496 None:intergenic 40.0%
AGCAGAACTAAATTGCGGTT+CGG - chr1_2:19457477-19457496 Msa0061380:CDS 40.0%
GAGATATTGATCCAAAGCCA+AGG - chr1_2:19457532-19457551 Msa0061380:CDS 40.0%
!! TTTTGGCTGTCAGATCTCAA+AGG - chr1_2:19457590-19457609 Msa0061380:CDS 40.0%
! GTGGATGCTAGTGTAGATTT+TGG - chr1_2:19457636-19457655 Msa0061380:CDS 40.0%
!!! GCTAGTGTAGATTTTGGGAT+TGG - chr1_2:19457642-19457661 Msa0061380:CDS 40.0%
! TTGGGATTGGTATGAGAAAG+TGG - chr1_2:19457655-19457674 Msa0061380:CDS 40.0%
! TACTTTGCAGTTTTACCCTG+TGG + chr1_2:19457762-19457781 None:intergenic 40.0%
! GTAGAGCATTATACCGGTGA+AGG - chr1_2:19457345-19457364 Msa0061380:CDS 45.0%
TTATACCGGTGAAGGAAGTG+TGG - chr1_2:19457353-19457372 Msa0061380:CDS 45.0%
GCAGAACTAAATTGCGGTTC+GGG - chr1_2:19457478-19457497 Msa0061380:CDS 45.0%
AAACTGTCCAAGAATGCCCT+TGG + chr1_2:19457552-19457571 None:intergenic 45.0%
ATTGAGCTGCATCAACCACA+GGG - chr1_2:19457744-19457763 Msa0061380:CDS 45.0%
! GTAGAGCATTATACCGGTGA+AGG - chr1_2:19457345-19457364 Msa0061380:CDS 45.0%
TTATACCGGTGAAGGAAGTG+TGG - chr1_2:19457353-19457372 Msa0061380:CDS 45.0%
GCAGAACTAAATTGCGGTTC+GGG - chr1_2:19457478-19457497 Msa0061380:CDS 45.0%
AAACTGTCCAAGAATGCCCT+TGG + chr1_2:19457552-19457571 None:intergenic 45.0%
ATTGAGCTGCATCAACCACA+GGG - chr1_2:19457744-19457763 Msa0061380:CDS 45.0%
!!! AACAAAATTTAAAATAAAAA+AGG + chr1_2:19457849-19457868 None:intergenic 5.0%
!!! TAATAGATATATATATTTTT+AGG - chr1_2:19457957-19457976 Msa0061380:intron 5.0%
!!! AACAAAATTTAAAATAAAAA+AGG + chr1_2:19457849-19457868 None:intergenic 5.0%
!!! TAATAGATATATATATTTTT+AGG - chr1_2:19457957-19457976 Msa0061380:intron 5.0%
AGCAACCACACTTCCTTCAC+CGG + chr1_2:19457361-19457380 None:intergenic 50.0%
!! TCCAAAGCCAAGGGCATTCT+TGG - chr1_2:19457542-19457561 Msa0061380:CDS 50.0%
TCCAAGAATGCCCTTGGCTT+TGG + chr1_2:19457546-19457565 None:intergenic 50.0%
GATTGAGCTGCATCAACCAC+AGG - chr1_2:19457743-19457762 Msa0061380:CDS 50.0%
AGCAACCACACTTCCTTCAC+CGG + chr1_2:19457361-19457380 None:intergenic 50.0%
!! TCCAAAGCCAAGGGCATTCT+TGG - chr1_2:19457542-19457561 Msa0061380:CDS 50.0%
TCCAAGAATGCCCTTGGCTT+TGG + chr1_2:19457546-19457565 None:intergenic 50.0%
GATTGAGCTGCATCAACCAC+AGG - chr1_2:19457743-19457762 Msa0061380:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 19457102 19458037 19457102 ID=Msa0061380;Name=Msa0061380
chr1_2 mRNA 19457102 19458037 19457102 ID=Msa0061380-mRNA-1;Parent=Msa0061380;Name=Msa0061380-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0.5|0.5|1|1|2|177|205
chr1_2 exon 19457102 19457892 19457102 ID=Msa0061380-mRNA-1:exon:7570;Parent=Msa0061380-mRNA-1
chr1_2 exon 19458034 19458037 19458034 ID=Msa0061380-mRNA-1:exon:7569;Parent=Msa0061380-mRNA-1
chr1_2 CDS 19458034 19458037 19458034 ID=Msa0061380-mRNA-1:cds;Parent=Msa0061380-mRNA-1
chr1_2 CDS 19457279 19457892 19457279 ID=Msa0061380-mRNA-1:cds;Parent=Msa0061380-mRNA-1
chr1_2 three_prime_UTR 19457102 19457278 19457102 ID=Msa0061380-mRNA-1:three_prime_utr;Parent=Msa0061380-mRNA-1
Gene Sequence

>Msa0061380

ATGAGAAAGTCAAAAAAGTTAGCACTGAAGATGGGTAAAAGTAAAACAATTGAGTTGTATGGATTCCCTTCTTTTGTGAACGTGTCTCAAGTAAAGATATTTGTAGAGCATTATACCGGTGAAGGAAGTGTGGTTGCTATGAAGATTAGAGTAGGCAAAGGACAGAATCCAAGAGCATTTGCAATTATTCAATTCACTGCTGCAAAACATGCTACATTCATGATGGCCTTACCTAGCAGAACTAAATTGCGGTTCGGGAACTCCTATTTAAAAGTTAGGGAAATGGAAAGAGATATTGATCCAAAGCCAAGGGCATTCTTGGACAGTTTAGACGATGTTAAACTGTATTTTGGCTGTCAGATCTCAAAGGAAAGATTCTCTATATTATGGAAGGTGGATGCTAGTGTAGATTTTGGGATTGGTATGAGAAAGTGGCGATTCTCTATGCTCCATGAAAATAAGAAGTTCAAACTTGAGCTTTCATATGAGAATATTTGGAAGATTGAGCTGCATCAACCACAGGGTAAAACTGCAAAGTATCTATTGATTCAGGTTGTTAAATTCAACCTTTCATTAAATATTTTAAACAATGTTCTTCATTTTCCTTTTTTATTTTAA

Protein sequence

>Msa0061380

MRKSKKLALKMGKSKTIELYGFPSFVNVSQVKIFVEHYTGEGSVVAMKIRVGKGQNPRAFAIIQFTAAKHATFMMALPSRTKLRFGNSYLKVREMERDIDPKPRAFLDSLDDVKLYFGCQISKERFSILWKVDASVDFGIGMRKWRFSMLHENKKFKLELSYENIWKIELHQPQGKTAKYLLIQVVKFNLSLNILNNVLHFPFLF*