AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0967670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0967670 MtrunA17_Chr3g0127091 94.015 401 12 3 1 400 1 390 0.0 761
Msa0967670 MtrunA17_Chr2g0293811 32.492 317 152 10 57 356 57 328 1.06e-35 134
Msa0967670 MtrunA17_Chr4g0001761 29.882 338 162 10 60 356 22 325 3.30e-31 122
Msa0967670 MtrunA17_Chr1g0194191 30.312 320 167 9 58 363 76 353 5.56e-31 122
Msa0967670 MtrunA17_Chr4g0065141 28.617 311 165 8 58 356 82 347 5.72e-30 119
Msa0967670 MtrunA17_Chr5g0407161 28.846 312 164 7 60 361 84 347 1.11e-29 118
Msa0967670 MtrunA17_Chr1g0150071 28.691 359 182 8 60 377 22 347 3.22e-29 116
Msa0967670 MtrunA17_Chr3g0136811 26.810 373 179 8 60 381 22 351 9.26e-29 115
Msa0967670 MtrunA17_Chr3g0138281 26.129 310 172 7 58 356 79 342 1.27e-27 112
Msa0967670 MtrunA17_Chr1g0150451 27.044 318 167 9 47 356 60 320 1.31e-27 111
Msa0967670 MtrunA17_Chr3g0136711 26.899 316 166 9 49 356 19 277 1.49e-27 110
Msa0967670 MtrunA17_Chr1g0212961 29.703 303 145 10 60 356 94 334 1.94e-27 111
Msa0967670 MtrunA17_Chr5g0430481 29.236 301 148 11 68 359 130 374 3.68e-26 108
Msa0967670 MtrunA17_Chr3g0126511 27.759 299 154 8 63 356 96 337 3.58e-25 105
Msa0967670 MtrunA17_Chr6g0483411 28.754 313 151 9 68 356 51 315 4.81e-25 104
Msa0967670 MtrunA17_Chr3g0112291 25.680 331 167 8 61 359 92 375 6.66e-25 105
Msa0967670 MtrunA17_Chr3g0105371 27.723 303 150 9 68 359 94 338 3.45e-24 102
Msa0967670 MtrunA17_Chr3g0135961 24.930 357 172 11 56 359 226 539 2.84e-23 101
Msa0967670 MtrunA17_Chr2g0317021 27.365 296 145 10 73 359 98 332 4.57e-22 96.3
Msa0967670 MtrunA17_Chr1g0158781 29.630 324 157 12 59 362 110 382 5.87e-22 96.7
Msa0967670 MtrunA17_Chr1g0171571 27.500 320 155 12 58 359 384 644 6.43e-22 97.8
Msa0967670 MtrunA17_Chr4g0070871 24.928 345 164 9 62 359 207 503 9.12e-22 97.1
Msa0967670 MtrunA17_Chr4g0050381 27.273 308 153 11 59 356 27 273 9.75e-22 94.4
Msa0967670 MtrunA17_Chr3g0105351 25.688 327 171 10 47 359 46 314 1.90e-21 94.0
Msa0967670 MtrunA17_Chr8g0360351 27.574 272 129 8 92 356 68 278 3.16e-21 92.8
Msa0967670 MtrunA17_Chr1g0150781 25.356 351 162 11 62 359 240 543 2.42e-20 93.2
Msa0967670 MtrunA17_Chr5g0396731 27.759 299 159 8 42 329 53 305 3.36e-20 91.3
Msa0967670 MtrunA17_Chr2g0279151 26.948 308 154 11 59 356 31 277 3.19e-19 87.0
Msa0967670 MtrunA17_Chr2g0299981 25.205 365 170 13 54 359 215 535 1.20e-18 87.8
Msa0967670 MtrunA17_Chr8g0389921 23.739 337 171 9 60 359 147 434 1.79e-17 84.0
Msa0967670 MtrunA17_Chr4g0068971 32.121 165 79 3 210 372 113 246 2.50e-17 81.3
Msa0967670 MtrunA17_Chr4g0051661 24.828 290 140 10 74 344 165 395 2.87e-17 82.8
Msa0967670 MtrunA17_Chr5g0435721 24.468 282 149 7 61 327 192 424 4.40e-17 82.8
Msa0967670 MtrunA17_Chr7g0256751 26.923 286 173 9 90 363 69 330 2.44e-16 80.5
Msa0967670 MtrunA17_Chr3g0089721 24.367 316 174 10 90 368 68 355 2.51e-14 73.9
Msa0967670 MtrunA17_Chr3g0089711 23.975 317 176 10 90 369 68 356 6.64e-14 72.4
Msa0967670 MtrunA17_Chr7g0256771 25.660 265 152 9 90 337 70 306 3.54e-13 69.7
Msa0967670 MtrunA17_Chr7g0263281 25.856 263 138 10 89 328 70 298 5.41e-12 66.6
Msa0967670 MtrunA17_Chr1g0211251 24.092 303 167 7 44 327 49 307 6.16e-12 66.6
Msa0967670 MtrunA17_Chr1g0207571 25.984 254 139 7 89 322 60 284 1.47e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0967670 AT4G27800.1 62.687 402 133 5 1 399 1 388 0.0 509
Msa0967670 AT4G27800.2 62.248 347 114 5 1 344 1 333 2.01e-150 429
Msa0967670 AT4G27800.3 61.062 339 115 5 1 336 1 325 2.79e-144 413
Msa0967670 AT3G62260.1 29.204 339 182 9 51 372 67 364 4.83e-33 128
Msa0967670 AT3G62260.2 29.204 339 182 9 51 372 68 365 5.33e-33 127
Msa0967670 AT1G43900.1 31.229 301 142 10 62 356 124 365 4.10e-30 119
Msa0967670 AT3G62260.3 28.621 290 161 7 89 372 26 275 5.05e-30 117
Msa0967670 AT3G51470.2 27.213 305 167 6 58 356 25 280 9.21e-30 117
Msa0967670 AT3G51470.1 27.213 305 167 6 58 356 69 324 1.92e-29 117
Msa0967670 AT5G53140.1 27.217 327 175 9 56 377 96 364 9.57e-28 114
Msa0967670 AT5G53140.2 27.217 327 175 9 56 377 96 364 9.57e-28 114
Msa0967670 AT2G25620.1 26.198 313 178 7 58 359 86 356 1.60e-27 112
Msa0967670 AT2G25620.2 26.198 313 178 7 58 359 86 356 1.60e-27 112
Msa0967670 AT2G33700.1 26.380 326 182 5 58 373 81 358 3.86e-27 111
Msa0967670 AT5G24940.1 27.129 317 166 9 48 356 17 276 5.40e-27 112
Msa0967670 AT2G25070.1 27.479 353 162 9 60 361 22 331 6.80e-27 110
Msa0967670 AT2G25070.2 27.479 353 162 9 60 361 22 331 6.80e-27 110
Msa0967670 AT4G31750.2 28.975 283 140 8 49 323 18 247 1.09e-26 108
Msa0967670 AT1G48040.1 28.354 328 180 10 58 372 75 360 1.51e-26 110
Msa0967670 AT4G31750.1 28.975 283 140 8 49 323 18 247 1.95e-26 108
Msa0967670 AT4G31750.3 28.975 283 140 8 49 323 18 247 1.95e-26 108
Msa0967670 AT3G17250.1 28.571 336 177 13 41 356 98 390 2.57e-26 110
Msa0967670 AT1G67820.2 31.599 269 120 8 62 322 85 297 1.50e-25 107
Msa0967670 AT5G59220.1 27.517 298 140 7 61 335 111 355 1.63e-25 107
Msa0967670 AT5G10740.1 26.814 317 167 9 48 356 17 276 1.64e-25 106
Msa0967670 AT5G10740.2 26.814 317 167 9 48 356 17 276 1.64e-25 106
Msa0967670 AT1G67820.1 31.599 269 120 8 62 322 121 333 2.26e-25 107
Msa0967670 AT2G29380.1 26.888 331 163 9 61 359 77 360 2.89e-25 105
Msa0967670 AT2G40180.1 30.132 302 150 11 68 359 135 385 4.57e-25 105
Msa0967670 AT1G18030.1 29.221 308 156 9 68 356 81 345 4.87e-25 105
Msa0967670 AT1G07160.1 27.729 339 165 11 44 359 95 376 1.05e-24 104
Msa0967670 AT2G40860.4 27.469 324 169 10 47 359 282 550 7.13e-24 103
Msa0967670 AT2G40860.1 27.778 324 168 10 47 359 380 648 7.67e-24 104
Msa0967670 AT2G40860.2 28.571 308 155 10 47 341 380 635 1.38e-22 100
Msa0967670 AT2G30020.1 27.575 301 155 9 68 359 146 392 6.27e-22 97.1
Msa0967670 AT4G28400.3 26.027 292 146 9 74 356 27 257 6.75e-22 94.7
Msa0967670 AT4G28400.1 26.027 292 146 9 74 356 49 279 1.13e-21 94.4
Msa0967670 AT1G07430.1 24.457 368 166 11 55 359 115 433 1.54e-21 96.3
Msa0967670 AT3G15260.2 26.962 293 144 12 73 356 54 285 3.99e-21 93.2
Msa0967670 AT3G15260.1 26.962 293 144 12 73 356 54 285 3.99e-21 93.2
Msa0967670 AT2G20630.1 26.370 292 145 10 74 356 45 275 4.30e-20 90.1
Msa0967670 AT2G20630.2 26.370 292 145 10 74 356 45 275 6.66e-20 89.7
Msa0967670 AT5G57050.2 24.277 346 167 10 62 359 73 371 1.37e-19 90.1
Msa0967670 AT5G57050.1 24.277 346 167 10 62 359 113 411 2.02e-19 90.1
Msa0967670 AT3G11410.1 27.243 301 156 9 49 330 94 350 2.72e-19 89.4
Msa0967670 AT1G18030.2 29.771 262 123 9 68 311 81 299 4.24e-19 87.4
Msa0967670 AT2G34740.1 26.621 293 143 12 74 356 101 331 5.28e-19 87.8
Msa0967670 AT1G72770.4 24.384 365 167 12 56 359 185 501 1.02e-18 88.2
Msa0967670 AT1G72770.1 24.384 365 167 12 56 359 185 501 1.02e-18 88.2
Msa0967670 AT1G72770.3 24.384 365 167 12 56 359 185 501 1.02e-18 88.2
Msa0967670 AT5G59220.2 28.922 204 106 6 61 241 111 298 6.34e-18 84.0
Msa0967670 AT4G26080.1 23.729 354 177 11 62 370 129 434 6.62e-18 85.5
Msa0967670 AT1G78200.1 24.918 305 165 10 60 356 31 279 3.03e-17 82.0
Msa0967670 AT1G78200.3 24.918 305 165 10 60 356 31 279 3.03e-17 82.0
Msa0967670 AT1G78200.2 24.918 305 165 10 60 356 31 279 3.03e-17 82.0
Msa0967670 AT1G78200.5 25.172 290 154 9 74 356 1 234 5.21e-17 80.1
Msa0967670 AT1G78200.4 25.172 290 154 9 74 356 1 234 5.21e-17 80.1
Msa0967670 AT1G22280.3 23.962 313 169 10 56 356 28 283 1.80e-16 79.7
Msa0967670 AT1G22280.1 24.437 311 164 10 56 356 28 277 2.58e-16 79.0
Msa0967670 AT5G51760.2 23.876 356 173 9 55 359 73 381 2.88e-16 80.1
Msa0967670 AT5G51760.1 23.876 356 173 9 55 359 103 411 4.41e-16 80.1
Msa0967670 AT1G34750.2 25.240 313 157 12 60 359 33 281 1.23e-14 74.3
Msa0967670 AT1G34750.1 25.240 313 157 12 60 359 33 281 1.23e-14 74.3
Msa0967670 AT1G34750.3 25.240 313 157 12 60 359 33 281 1.23e-14 74.3
Msa0967670 AT2G40860.3 27.612 268 131 9 47 303 380 595 8.64e-14 73.6
Msa0967670 AT5G02760.1 24.242 297 174 8 44 328 40 297 5.22e-13 70.5
Msa0967670 AT2G34740.3 25.911 247 115 10 74 310 97 285 7.59e-13 69.3
Msa0967670 AT2G34740.2 25.911 247 115 10 74 310 115 303 8.46e-13 69.3
Msa0967670 AT5G57050.3 28.293 205 111 7 62 240 113 307 8.60e-13 69.3
Msa0967670 AT5G06750.3 25.746 268 139 9 86 327 78 311 1.70e-12 68.9
Msa0967670 AT5G06750.1 25.746 268 139 9 86 327 78 311 1.70e-12 68.9
Msa0967670 AT4G38520.2 24.521 261 144 6 89 328 80 308 3.02e-12 68.2
Msa0967670 AT4G38520.1 24.521 261 144 6 89 328 80 308 3.02e-12 68.2
Msa0967670 AT3G17090.1 26.172 256 144 11 89 327 83 310 3.89e-12 67.8
Msa0967670 AT3G17090.2 26.172 256 144 11 89 327 83 310 4.18e-12 67.0
Msa0967670 AT5G19280.1 27.431 288 144 12 88 359 339 577 9.33e-12 67.0
Msa0967670 AT3G51370.1 24.125 257 148 9 89 327 78 305 3.36e-11 64.7
Msa0967670 AT3G51370.3 24.125 257 148 9 89 327 78 305 3.36e-11 64.7
Msa0967670 AT1G34750.4 25.670 261 127 10 60 310 33 236 3.93e-11 63.2

Find 114 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAGAGGTGGAGTGTTTA+TGG 0.113064 6_3:+97773818 None:intergenic
TCTGTTTGTGTTTATGTATT+TGG 0.232802 6_3:+97773798 None:intergenic
GAGAAACCTTGGAGACTATT+TGG 0.260980 6_3:+97773622 None:intergenic
CTTATTATTTCCTAGGAATA+TGG 0.264600 6_3:+97772734 None:intergenic
TGTTAGCATCCGATGGCTTA+TGG 0.271564 6_3:-97770661 Msa0967670:CDS
TGAGGACTAGTTAGCAATTC+TGG 0.276029 6_3:+97772183 None:intergenic
CTGACATGAGGTTGTTAAAA+TGG 0.280042 6_3:-97772917 Msa0967670:intron
GCTTCAAACTTGAAAGCTTT+AGG 0.297676 6_3:-97773890 Msa0967670:five_prime_UTR
TTGCTGTATCCCGTGCATTT+GGG 0.314251 6_3:-97771086 Msa0967670:CDS
AAGCTGCTAATTTCACATAT+TGG 0.316833 6_3:-97772714 Msa0967670:CDS
ATTGCTGTATCCCGTGCATT+TGG 0.322501 6_3:-97771087 Msa0967670:CDS
TGGAAGATGATATTATTCTT+AGG 0.339370 6_3:-97773645 Msa0967670:CDS
AGAGTTACTTGATAAATATC+AGG 0.343230 6_3:+97770707 None:intergenic
TCTTTCGCGGCCGTGTTTGA+TGG 0.343544 6_3:-97773601 Msa0967670:CDS
AACATTTAAAAGAAAATATC+AGG 0.346106 6_3:+97769651 None:intergenic
GAATTGTGTCTATTGGAATC+TGG 0.357830 6_3:-97769815 Msa0967670:CDS
ACCGTTGGAATTGTGTCTAT+TGG 0.358693 6_3:-97769822 Msa0967670:CDS
CAGACTCTCCTCCTATTAGA+TGG 0.360549 6_3:-97773702 Msa0967670:CDS
AATCCGTTAAAATCAGTTAG+AGG 0.362802 6_3:-97774011 Msa0967670:five_prime_UTR
ATCCCTTGCACAAGTCGTTT+TGG 0.370646 6_3:-97770376 Msa0967670:intron
AGCATTATAATTGCCGATCT+AGG 0.371221 6_3:-97770251 Msa0967670:CDS
GAGAGAGAGAGTTAGATAAC+AGG 0.372425 6_3:+97773978 None:intergenic
GATACTGATCCCCATCTAAT+AGG 0.373259 6_3:+97773691 None:intergenic
TTAGCAGCTTATTATTTCCT+AGG 0.379640 6_3:+97772727 None:intergenic
GTTTATGGAGCTTAGTTAAG+AGG 0.385365 6_3:+97773833 None:intergenic
ACTAGTCCTCACCGTCCATA+TGG 0.386128 6_3:-97772171 Msa0967670:CDS
GTTAGCATCCGATGGCTTAT+GGG 0.389003 6_3:-97770660 Msa0967670:CDS
ATGGGGATCAGTATCTTTGC+AGG 0.390272 6_3:-97773683 Msa0967670:CDS
TTGCTTCTGTCCAGTTCCTC+AGG 0.392143 6_3:-97773567 Msa0967670:intron
TTTAAAGGCTTGAAATGAAA+GGG 0.400684 6_3:-97772791 Msa0967670:intron
GTGAAAAGGATGCTACAAAA+AGG 0.413005 6_3:-97770917 Msa0967670:CDS
ATAGCTAAGACCTGAGGAAC+TGG 0.414366 6_3:+97773557 None:intergenic
GGGGAGGAGGAGGATGAATC+AGG 0.415467 6_3:-97772771 Msa0967670:CDS
TGAAATGAAAGGGGAGGAGG+AGG 0.428058 6_3:-97772781 Msa0967670:CDS
GATATTTATCAAGTAACTCT+TGG 0.438968 6_3:-97770704 Msa0967670:CDS
ATCAAAAGAATCAGAGAAGC+AGG 0.445855 6_3:-97772123 Msa0967670:CDS
GGACAGAAAAGTTCATTTCC+CGG 0.447424 6_3:-97770877 Msa0967670:intron
GCAGGGTCCACGTGATGAAA+TGG 0.449478 6_3:-97773665 Msa0967670:CDS
AAGAGGAATCTTTGTATATG+TGG 0.453079 6_3:+97773850 None:intergenic
GAGGCCTTGTTGCTCCCATA+TGG 0.455386 6_3:+97772156 None:intergenic
TTCCAACGGTGGCTAGGGCT+TGG 0.460560 6_3:+97769835 None:intergenic
TGTGTTTATGTATTTGGAAG+AGG 0.466639 6_3:+97773804 None:intergenic
CACAATTCCAACGGTGGCTA+GGG 0.467000 6_3:+97769830 None:intergenic
GCGGCCGTGTTTGATGGCCA+TGG 0.471142 6_3:-97773595 Msa0967670:CDS
AAGTTTGAAGCTAAAGATGA+AGG 0.471540 6_3:+97773902 None:intergenic
GAAGTAAAGAAGTATGCATC+TGG 0.475549 6_3:+97769740 None:intergenic
GCTTGAAATGAAAGGGGAGG+AGG 0.477022 6_3:-97772784 Msa0967670:CDS
ATTACCAACGGTAGGATTTG+CGG 0.480678 6_3:-97771114 Msa0967670:CDS
CCGTGTTTGATGGCCATGGA+GGG 0.482647 6_3:-97773591 Msa0967670:CDS
ACATCTTAGTAGAATCTACT+TGG 0.485502 6_3:-97769686 Msa0967670:three_prime_UTR
ACACAATTCCAACGGTGGCT+AGG 0.491960 6_3:+97769829 None:intergenic
TCTCCAAGGTTTCTCTTTCG+CGG 0.496528 6_3:-97773614 Msa0967670:CDS
ACAGCAACTGCCATATTCCT+AGG 0.507970 6_3:-97772744 Msa0967670:CDS
AACTTGAAAGCTTTAGGTGA+TGG 0.512962 6_3:-97773884 Msa0967670:exon
CTTAGGCCAAATAGTCTCCA+AGG 0.519183 6_3:-97773628 Msa0967670:CDS
GCTTATGGGATTACATGAGC+AGG 0.519531 6_3:-97770646 Msa0967670:intron
ATGTACACTAAATATTATAC+AGG 0.519730 6_3:+97769714 None:intergenic
CTAGTCCTCACCGTCCATAT+GGG 0.520919 6_3:-97772170 Msa0967670:CDS
GAATCAGAGAAGCAGGTGGA+TGG 0.523820 6_3:-97772116 Msa0967670:CDS
AAGGAGCTCAGGAAGGAAGA+TGG 0.526606 6_3:-97770898 Msa0967670:CDS
TGCTGTATCCCGTGCATTTG+GGG 0.527648 6_3:-97771085 Msa0967670:CDS
GCATTATAATTGCCGATCTA+GGG 0.535217 6_3:-97770250 Msa0967670:intron
AGTTTGAAGCTAAAGATGAA+GGG 0.543295 6_3:+97773903 None:intergenic
AGACTCTCCTCCTATTAGAT+GGG 0.543447 6_3:-97773701 Msa0967670:CDS
TTTATAGTGTTAGCATCCGA+TGG 0.544491 6_3:-97770668 Msa0967670:CDS
AACGGATTGGATTCTCTCGG+TGG 0.545973 6_3:+97774026 None:intergenic
GTGCTTGCAGGAAGACAGAC+TGG 0.546345 6_3:-97769902 Msa0967670:intron
GGAGAGTCTGTCAATGATGA+AGG 0.549099 6_3:+97773715 None:intergenic
CGGCCGCGAAAGAGAAACCT+TGG 0.549935 6_3:+97773611 None:intergenic
CTCAGTTCAGATACTGAAGA+AGG 0.550057 6_3:+97773942 None:intergenic
GTCCAAGCCCTAGCCACCGT+TGG 0.555090 6_3:-97769837 Msa0967670:CDS
CAATGATGAAGGTGCATCAA+TGG 0.556018 6_3:+97773726 None:intergenic
ACAAAATACATACCCTAGAT+CGG 0.557827 6_3:+97770238 None:intergenic
ATCGCATGTCCCCAAATGCA+CGG 0.559501 6_3:+97771076 None:intergenic
TTTAACGGATTGGATTCTCT+CGG 0.562239 6_3:+97774023 None:intergenic
CCCTCCATGGCCATCAAACA+CGG 0.563996 6_3:+97773591 None:intergenic
TGTACAGTTCAAAGATGACT+TGG 0.565147 6_3:-97770741 Msa0967670:CDS
AATCAGAGAAGCAGGTGGAT+GGG 0.572153 6_3:-97772115 Msa0967670:intron
GTTTATGTATTTGGAAGAGG+TGG 0.576117 6_3:+97773807 None:intergenic
TGTTGAAGAAGCATTAAAGC+AGG 0.576690 6_3:-97772952 Msa0967670:CDS
TCGCATGTCCCCAAATGCAC+GGG 0.578399 6_3:+97771077 None:intergenic
TACCAAAACGACTTGTGCAA+GGG 0.580364 6_3:+97770374 None:intergenic
GAGTGTCTTGAAGCTCTACA+AGG 0.580723 6_3:-97773008 Msa0967670:CDS
TTACCAAAACGACTTGTGCA+AGG 0.581838 6_3:+97770373 None:intergenic
TTAAAGGCTTGAAATGAAAG+GGG 0.583830 6_3:-97772790 Msa0967670:intron
ACACTAAATATTATACAGGC+AGG 0.583996 6_3:+97769718 None:intergenic
CTACAGGGATGAGTTATACA+AGG 0.587623 6_3:-97773030 Msa0967670:intron
GGACAGAAGCAAACCCTCCA+TGG 0.588408 6_3:+97773578 None:intergenic
ATCTCCGCAAATCCTACCGT+TGG 0.588610 6_3:+97771110 None:intergenic
GAGTTACTTGATAAATATCA+GGG 0.596312 6_3:+97770708 None:intergenic
ACTGATCCCCATCTAATAGG+AGG 0.597752 6_3:+97773694 None:intergenic
GTCTTGAAGCTCTACAAGGT+GGG 0.604160 6_3:-97773004 Msa0967670:CDS
CATGTAATCCCATAAGCCAT+CGG 0.609703 6_3:+97770652 None:intergenic
GCCGTGTTTGATGGCCATGG+AGG 0.611537 6_3:-97773592 Msa0967670:CDS
AAGGCTTGAAATGAAAGGGG+AGG 0.613590 6_3:-97772787 Msa0967670:CDS
TGAAGGTGCATCAATGGCTA+TGG 0.614608 6_3:+97773732 None:intergenic
ACATATTGGTGACTCATCTG+TGG 0.614996 6_3:-97772700 Msa0967670:intron
TCATCTTCCATTTCATCACG+TGG 0.615775 6_3:+97773658 None:intergenic
TGGGGATCAGTATCTTTGCA+GGG 0.616882 6_3:-97773682 Msa0967670:CDS
TTCTTTAGATTACCAACGGT+AGG 0.622788 6_3:-97771122 Msa0967670:intron
CCTTGTTGCTCCCATATGGA+CGG 0.623217 6_3:+97772160 None:intergenic
GACTCTCCTCCTATTAGATG+GGG 0.625445 6_3:-97773700 Msa0967670:CDS
TGGCTATGGCAGAACAGCAA+CGG 0.629952 6_3:+97773746 None:intergenic
CCGTCCATATGGGAGCAACA+AGG 0.639612 6_3:-97772160 Msa0967670:CDS
CATTTGTTAAAGCTGACATG+AGG 0.639772 6_3:-97772929 Msa0967670:CDS
TCTGCAGGACCAACGTACAC+AGG 0.646615 6_3:-97770282 Msa0967670:intron
AGGGATCAACTACGAAAACA+TGG 0.647059 6_3:-97770512 Msa0967670:CDS
TCCAATAGACACAATTCCAA+CGG 0.647966 6_3:+97769821 None:intergenic
TTGCTCCCATATGGACGGTG+AGG 0.659175 6_3:+97772165 None:intergenic
AAAAGAATCAGAGAAGCAGG+TGG 0.661475 6_3:-97772120 Msa0967670:CDS
TGTCTTGAAGCTCTACAAGG+TGG 0.664593 6_3:-97773005 Msa0967670:CDS
ACGAAAACATGGAAACACAC+AGG 0.687122 6_3:-97770501 Msa0967670:intron
AATAGACACAATTCCAACGG+TGG 0.712558 6_3:+97769824 None:intergenic
TGACATTATCCTGTGTACGT+TGG 0.736320 6_3:+97770273 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATGAAAATAAAATTC+TGG - chr6_3:97771727-97771746 Msa0967670:intron 10.0%
!! ATTAATTAATTATGTGTGTT+TGG - chr6_3:97770114-97770133 Msa0967670:intron 15.0%
!! TTAATTAATTATGTGTGTTT+GGG - chr6_3:97770115-97770134 Msa0967670:intron 15.0%
!! AAATTTACAAAATCAATACA+AGG + chr6_3:97770162-97770181 None:intergenic 15.0%
!!! TTAATCAATTTTATTCATGT+AGG - chr6_3:97771389-97771408 Msa0967670:intron 15.0%
!!! ATAATTAATCTGATGTTTTA+TGG - chr6_3:97771786-97771805 Msa0967670:intron 15.0%
!!! TAATTAATCTGATGTTTTAT+GGG - chr6_3:97771787-97771806 Msa0967670:intron 15.0%
!!! TCATTTTGAATAATTTGTTT+AGG - chr6_3:97771975-97771994 Msa0967670:intron 15.0%
!!! CTTTTTTAATAATATTGGAT+AGG + chr6_3:97772024-97772043 None:intergenic 15.0%
!!! CATCTCTTTTTTAATAATAT+TGG + chr6_3:97772029-97772048 None:intergenic 15.0%
!! TTTGAATTTCATTGATATTT+AGG - chr6_3:97772252-97772271 Msa0967670:intron 15.0%
!!! TTTTATTTAATTTACCTTGA+TGG - chr6_3:97772374-97772393 Msa0967670:intron 15.0%
!!! TTTTTTTTTTCTTTTCACAA+AGG + chr6_3:97773460-97773479 None:intergenic 15.0%
!! AACATTTAAAAGAAAATATC+AGG + chr6_3:97773967-97773986 None:intergenic 15.0%
!! TAATTAATTATGTGTGTTTG+GGG - chr6_3:97770116-97770135 Msa0967670:intron 20.0%
!! TAGTTAAAAGCTTATAACAT+AGG - chr6_3:97770279-97770298 Msa0967670:intron 20.0%
!!! ATAACAAATCTAGAATCATT+TGG + chr6_3:97770765-97770784 None:intergenic 20.0%
!!! ATTTGTTATGATTACATTGA+AGG - chr6_3:97770776-97770795 Msa0967670:intron 20.0%
!!! AGACAGTATTTTGTTTTTAA+AGG - chr6_3:97770809-97770828 Msa0967670:intron 20.0%
!!! AAAACCAATTTTGTATTCTT+AGG - chr6_3:97771030-97771049 Msa0967670:intron 20.0%
!!! TTCAAAAAATGGAAAAGTAT+CGG + chr6_3:97771248-97771267 None:intergenic 20.0%
!! ATAAATCATACATTTGACTA+GGG - chr6_3:97771281-97771300 Msa0967670:intron 20.0%
!! ATATAAAAAATGAAGGAACA+AGG + chr6_3:97771534-97771553 None:intergenic 20.0%
!!! AAAGAGCATATAAAAAATGA+AGG + chr6_3:97771541-97771560 None:intergenic 20.0%
!!! AATTAATCTGATGTTTTATG+GGG - chr6_3:97771788-97771807 Msa0967670:intron 20.0%
!! GAAATATATTGTTTCCAATA+AGG - chr6_3:97771873-97771892 Msa0967670:intron 20.0%
!! AAACACAATCCTAATAAAAT+AGG - chr6_3:97772154-97772173 Msa0967670:CDS 20.0%
!!! TGATTTCATGATTTTGTTTT+GGG + chr6_3:97772199-97772218 None:intergenic 20.0%
!!! TTGATTTCATGATTTTGTTT+TGG + chr6_3:97772200-97772219 None:intergenic 20.0%
!! ATAAATTCCTTTATACTAGA+AGG + chr6_3:97772610-97772629 None:intergenic 20.0%
!! ATTGAATAAAACTCATATTC+TGG - chr6_3:97773492-97773511 Msa0967670:intron 20.0%
!! ATGTACACTAAATATTATAC+AGG + chr6_3:97773904-97773923 None:intergenic 20.0%
! TCTGTTTGTGTTTATGTATT+TGG + chr6_3:97769820-97769839 None:intergenic 25.0%
! TGGAAGATGATATTATTCTT+AGG - chr6_3:97769970-97769989 Msa0967670:intron 25.0%
! TGTTTCTTTATTATGAAAGC+CGG - chr6_3:97770232-97770251 Msa0967670:intron 25.0%
!! TTATTTTCATAAGTGGTACA+TGG + chr6_3:97770337-97770356 None:intergenic 25.0%
!!! TTTCAGCTTATTTTCATAAG+TGG + chr6_3:97770344-97770363 None:intergenic 25.0%
! TAGAGAATTTCAAATGTGTT+TGG + chr6_3:97770495-97770514 None:intergenic 25.0%
!!! TTTTAAAGGCTTGAAATGAA+AGG - chr6_3:97770823-97770842 Msa0967670:intron 25.0%
! TTTAAAGGCTTGAAATGAAA+GGG - chr6_3:97770824-97770843 Msa0967670:intron 25.0%
! CTTATTATTTCCTAGGAATA+TGG + chr6_3:97770884-97770903 None:intergenic 25.0%
! ACTACCTAAGAATACAAAAT+TGG + chr6_3:97771037-97771056 None:intergenic 25.0%
! CTACTTACTTAGTTACATAT+CGG - chr6_3:97771182-97771201 Msa0967670:intron 25.0%
! TACTTACTTAGTTACATATC+GGG - chr6_3:97771183-97771202 Msa0967670:intron 25.0%
!!! TCAAAAAATGGAAAAGTATC+GGG + chr6_3:97771247-97771266 None:intergenic 25.0%
! GTTGTAGATACTTCAAAAAA+TGG + chr6_3:97771259-97771278 None:intergenic 25.0%
! CATAAATCATACATTTGACT+AGG - chr6_3:97771280-97771299 Msa0967670:intron 25.0%
! ATCATTCAACTAGAATATCT+AGG - chr6_3:97771339-97771358 Msa0967670:intron 25.0%
! TCATTCAACTAGAATATCTA+GGG - chr6_3:97771340-97771359 Msa0967670:intron 25.0%
!!! TTTTATATGCTCTTTGAGTT+TGG - chr6_3:97771546-97771565 Msa0967670:intron 25.0%
!!! TTGAGTTTGGTCAATTTTTT+TGG - chr6_3:97771559-97771578 Msa0967670:intron 25.0%
!!! TTTTTGGTTTTAAAGAGTCT+AGG - chr6_3:97771575-97771594 Msa0967670:intron 25.0%
! GTCAAAAAGACATCAATTTA+TGG - chr6_3:97771914-97771933 Msa0967670:intron 25.0%
!!! TTTTTGCTTTTGTTTGAGTT+TGG + chr6_3:97772086-97772105 None:intergenic 25.0%
! CTAATATCCAACTACAAATT+AGG - chr6_3:97772222-97772241 Msa0967670:intron 25.0%
! TTCAAACTACACTTTATTGA+AGG - chr6_3:97772308-97772327 Msa0967670:intron 25.0%
!! TTGAAGGTCATTCTTTTAAT+TGG - chr6_3:97772324-97772343 Msa0967670:intron 25.0%
!!! TGAAGGTCATTCTTTTAATT+GGG - chr6_3:97772325-97772344 Msa0967670:intron 25.0%
! AAGGAATGATTTAGAGATTA+AGG + chr6_3:97772591-97772610 None:intergenic 25.0%
! AATCATTCCTTCTAGTATAA+AGG - chr6_3:97772600-97772619 Msa0967670:intron 25.0%
! GAGTTACTTGATAAATATCA+GGG + chr6_3:97772910-97772929 None:intergenic 25.0%
! AGAGTTACTTGATAAATATC+AGG + chr6_3:97772911-97772930 None:intergenic 25.0%
! GATATTTATCAAGTAACTCT+TGG - chr6_3:97772911-97772930 Msa0967670:intron 25.0%
! TGCCAAAAACTTTATATTTG+AGG - chr6_3:97773139-97773158 Msa0967670:intron 25.0%
!! TGCCTCAAATATAAAGTTTT+TGG + chr6_3:97773144-97773163 None:intergenic 25.0%
! TTGTCATGTATATATGACTT+TGG - chr6_3:97773396-97773415 Msa0967670:intron 25.0%
! TTGTTTAAATCCTAAACCTT+TGG + chr6_3:97773569-97773588 None:intergenic 25.0%
!! TCTAACTGATTTTAACGGAT+TGG + chr6_3:97769605-97769624 None:intergenic 30.0%
AATCCGTTAAAATCAGTTAG+AGG - chr6_3:97769604-97769623 Msa0967670:three_prime_UTR 30.0%
!! AAGCCTCTAACTGATTTTAA+CGG + chr6_3:97769610-97769629 None:intergenic 30.0%
! AGTTTGAAGCTAAAGATGAA+GGG + chr6_3:97769715-97769734 None:intergenic 30.0%
! AAGTTTGAAGCTAAAGATGA+AGG + chr6_3:97769716-97769735 None:intergenic 30.0%
AAGAGGAATCTTTGTATATG+TGG + chr6_3:97769768-97769787 None:intergenic 30.0%
TGTGTTTATGTATTTGGAAG+AGG + chr6_3:97769814-97769833 None:intergenic 30.0%
TAAGCTAACAAACATGAGAA+AGG + chr6_3:97770094-97770113 None:intergenic 30.0%
CTTATCCAAACAAATTACAC+CGG + chr6_3:97770254-97770273 None:intergenic 30.0%
!! ACTAAGCTTTAGAACATGTT+TGG + chr6_3:97770460-97770479 None:intergenic 30.0%
! GACACGTTTTATGTATTAAC+TGG + chr6_3:97770728-97770747 None:intergenic 30.0%
TTAAAGGCTTGAAATGAAAG+GGG - chr6_3:97770825-97770844 Msa0967670:intron 30.0%
TTAGCAGCTTATTATTTCCT+AGG + chr6_3:97770891-97770910 None:intergenic 30.0%
AAGCTGCTAATTTCACATAT+TGG - chr6_3:97770901-97770920 Msa0967670:CDS 30.0%
CTAAAGACACAAATGTCATT+TGG + chr6_3:97771083-97771102 None:intergenic 30.0%
ATGACATTTGTGTCTTTAGT+TGG - chr6_3:97771084-97771103 Msa0967670:CDS 30.0%
! TATCAGACATGTATCGATAT+TGG - chr6_3:97771209-97771228 Msa0967670:intron 30.0%
!! CAAAAAATGGAAAAGTATCG+GGG + chr6_3:97771246-97771265 None:intergenic 30.0%
CATTCAACTAGAATATCTAG+GGG - chr6_3:97771341-97771360 Msa0967670:intron 30.0%
!!! TCTTTTGATTTCTTGAAGAG+AGG + chr6_3:97771481-97771500 None:intergenic 30.0%
AAAATCACACTTACAAAGTG+TGG - chr6_3:97771602-97771621 Msa0967670:intron 30.0%
ATTATGAGTTGTGACAACTT+AGG - chr6_3:97771757-97771776 Msa0967670:intron 30.0%
! AATTGATGTCTTTTTGACAG+TGG + chr6_3:97771912-97771931 None:intergenic 30.0%
TCAGGTGATAGGAAATTAAT+TGG - chr6_3:97771952-97771971 Msa0967670:intron 30.0%
AGAAATAACAAAAACAGTCG+AGG - chr6_3:97772052-97772071 Msa0967670:intron 30.0%
GAAATAACAAAAACAGTCGA+GGG - chr6_3:97772053-97772072 Msa0967670:intron 30.0%
AAATAACAAAAACAGTCGAG+GGG - chr6_3:97772054-97772073 Msa0967670:intron 30.0%
!!! AGGATTGTGTTTTTAGAGAT+AGG + chr6_3:97772146-97772165 None:intergenic 30.0%
CCTAATAAAATAGGACAGTT+CGG - chr6_3:97772163-97772182 Msa0967670:CDS 30.0%
GACAACGTTCATTTCAAAAA+TGG + chr6_3:97772412-97772431 None:intergenic 30.0%
CGTGCTTTCATTATATAGAA+CGG + chr6_3:97772436-97772455 None:intergenic 30.0%
CGTTCTATATAATGAAAGCA+CGG - chr6_3:97772434-97772453 Msa0967670:intron 30.0%
! CCTTTTCTTTAGATTACCAA+CGG - chr6_3:97772489-97772508 Msa0967670:intron 30.0%
ACATACATAAGATTACCTAC+CGG + chr6_3:97772760-97772779 None:intergenic 30.0%
GAACAAGCAGAATATTATTG+CGG + chr6_3:97773199-97773218 None:intergenic 30.0%
ACAAAATACATACCCTAGAT+CGG + chr6_3:97773380-97773399 None:intergenic 30.0%
CAGCAATTACGTTCTATAAT+TGG - chr6_3:97773423-97773442 Msa0967670:intron 30.0%
TAAAACTCATATTCTGGTCA+TGG - chr6_3:97773498-97773517 Msa0967670:intron 30.0%
! ATATCACTAGAGCATTAATG+TGG - chr6_3:97773542-97773561 Msa0967670:intron 30.0%
ACACTAAATATTATACAGGC+AGG + chr6_3:97773900-97773919 None:intergenic 30.0%
ACATCTTAGTAGAATCTACT+TGG - chr6_3:97773929-97773948 Msa0967670:five_prime_UTR 30.0%
!! TTTAACGGATTGGATTCTCT+CGG + chr6_3:97769595-97769614 None:intergenic 35.0%
GCTTCAAACTTGAAAGCTTT+AGG - chr6_3:97769725-97769744 Msa0967670:three_prime_UTR 35.0%
!! AACTTGAAAGCTTTAGGTGA+TGG - chr6_3:97769731-97769750 Msa0967670:three_prime_UTR 35.0%
GTTTATGGAGCTTAGTTAAG+AGG + chr6_3:97769785-97769804 None:intergenic 35.0%
GTTTATGTATTTGGAAGAGG+TGG + chr6_3:97769811-97769830 None:intergenic 35.0%
TTGAATATAGCTAAGACCTG+AGG + chr6_3:97770067-97770086 None:intergenic 35.0%
TTTCATAAGTGGTACATGGT+AGG + chr6_3:97770333-97770352 None:intergenic 35.0%
AATAAGCTGAAAACAGCTCA+TGG - chr6_3:97770352-97770371 Msa0967670:intron 35.0%
AGATTGTTTGGGAAAGCTTA+TGG + chr6_3:97770396-97770415 None:intergenic 35.0%
GAACACTAGTGAGATTGTTT+GGG + chr6_3:97770407-97770426 None:intergenic 35.0%
TGAACACTAGTGAGATTGTT+TGG + chr6_3:97770408-97770427 None:intergenic 35.0%
!! GCTTTAGAACATGTTTGGAT+TGG + chr6_3:97770455-97770474 None:intergenic 35.0%
ATAGTGTGCATTTGCTCTTA+TGG + chr6_3:97770518-97770537 None:intergenic 35.0%
!! TGTTGAAGAAGCATTAAAGC+AGG - chr6_3:97770663-97770682 Msa0967670:CDS 35.0%
! CATTTGTTAAAGCTGACATG+AGG - chr6_3:97770686-97770705 Msa0967670:CDS 35.0%
CTGACATGAGGTTGTTAAAA+TGG - chr6_3:97770698-97770717 Msa0967670:CDS 35.0%
!! GTTTTATGAGAGGCACAATA+AGG + chr6_3:97770958-97770977 None:intergenic 35.0%
! CATTGTGCATGTTTTATGAG+AGG + chr6_3:97770968-97770987 None:intergenic 35.0%
!!! TTGGTTTTGTGACACATTCA+TGG + chr6_3:97771018-97771037 None:intergenic 35.0%
CTTAGGTAGTAATGTATCGA+AGG - chr6_3:97771047-97771066 Msa0967670:intron 35.0%
!! ATGTAGGTTTTATGCAGATC+TGG - chr6_3:97771405-97771424 Msa0967670:intron 35.0%
ATCAAAAGAATCAGAGAAGC+AGG - chr6_3:97771492-97771511 Msa0967670:intron 35.0%
CACTTGGTAAAACTAGGAAA+TGG + chr6_3:97771828-97771847 None:intergenic 35.0%
AACAATCAAACCACAACACT+TGG + chr6_3:97771844-97771863 None:intergenic 35.0%
GTAACTGATTGGAACCTTAT+TGG + chr6_3:97771890-97771909 None:intergenic 35.0%
! TTTTGACAGTGGTAACTGAT+TGG + chr6_3:97771901-97771920 None:intergenic 35.0%
TTAATTTCCTATCACCTGAC+AGG + chr6_3:97771951-97771970 None:intergenic 35.0%
! CCGAACTGTCCTATTTTATT+AGG + chr6_3:97772166-97772185 None:intergenic 35.0%
! ATGAGCACCTAATTTGTAGT+TGG + chr6_3:97772232-97772251 None:intergenic 35.0%
!! GGTCATTCTTTTAATTGGGT+TGG - chr6_3:97772329-97772348 Msa0967670:intron 35.0%
!!! TTACCTTGATGGTGCAATTT+TGG - chr6_3:97772385-97772404 Msa0967670:intron 35.0%
!! CCGTTGGTAATCTAAAGAAA+AGG + chr6_3:97772492-97772511 None:intergenic 35.0%
TTCTTTAGATTACCAACGGT+AGG - chr6_3:97772493-97772512 Msa0967670:intron 35.0%
!!! CACTTAGGAAGCATTTTAAC+AGG + chr6_3:97772661-97772680 None:intergenic 35.0%
GCAAAAGGTAGATAACACTT+AGG + chr6_3:97772676-97772695 None:intergenic 35.0%
GTGAAAAGGATGCTACAAAA+AGG - chr6_3:97772698-97772717 Msa0967670:intron 35.0%
CATACATAAGATTACCTACC+GGG + chr6_3:97772759-97772778 None:intergenic 35.0%
TGTACAGTTCAAAGATGACT+TGG - chr6_3:97772874-97772893 Msa0967670:intron 35.0%
TTTATAGTGTTAGCATCCGA+TGG - chr6_3:97772947-97772966 Msa0967670:CDS 35.0%
! AGGGTAGCAGAAATGATTTT+GGG + chr6_3:97773009-97773028 None:intergenic 35.0%
! AAGGGTAGCAGAAATGATTT+TGG + chr6_3:97773010-97773029 None:intergenic 35.0%
!!! TTCTGCTACCCTTCTTTTTT+TGG - chr6_3:97773017-97773036 Msa0967670:CDS 35.0%
ATTGTGAAGGTAATTCTCTG+CGG + chr6_3:97773045-97773064 None:intergenic 35.0%
AGCATTATAATTGCCGATCT+AGG - chr6_3:97773364-97773383 Msa0967670:intron 35.0%
GCATTATAATTGCCGATCTA+GGG - chr6_3:97773365-97773384 Msa0967670:intron 35.0%
!! TTCTTTTCACAAAGGCATAG+CGG + chr6_3:97773452-97773471 None:intergenic 35.0%
TTAATGTGGTCCAAAGGTTT+AGG - chr6_3:97773556-97773575 Msa0967670:intron 35.0%
AGCACTCAATATAAAACGCA+TGG + chr6_3:97773698-97773717 None:intergenic 35.0%
! GTTTTATATTGAGTGCTTGC+AGG - chr6_3:97773701-97773720 Msa0967670:CDS 35.0%
TCCAATAGACACAATTCCAA+CGG + chr6_3:97773797-97773816 None:intergenic 35.0%
GAATTGTGTCTATTGGAATC+TGG - chr6_3:97773800-97773819 Msa0967670:CDS 35.0%
! GAAGTAAAGAAGTATGCATC+TGG + chr6_3:97773878-97773897 None:intergenic 35.0%
GAGAGAGAGAGTTAGATAAC+AGG + chr6_3:97769640-97769659 None:intergenic 40.0%
CTCAGTTCAGATACTGAAGA+AGG + chr6_3:97769676-97769695 None:intergenic 40.0%
! CAATGATGAAGGTGCATCAA+TGG + chr6_3:97769892-97769911 None:intergenic 40.0%
AGACTCTCCTCCTATTAGAT+GGG - chr6_3:97769914-97769933 Msa0967670:intron 40.0%
GATACTGATCCCCATCTAAT+AGG + chr6_3:97769927-97769946 None:intergenic 40.0%
TCATCTTCCATTTCATCACG+TGG + chr6_3:97769960-97769979 None:intergenic 40.0%
GAGAAACCTTGGAGACTATT+TGG + chr6_3:97769996-97770015 None:intergenic 40.0%
!! GAAAGCCGGTGTAATTTGTT+TGG - chr6_3:97770246-97770265 Msa0967670:intron 40.0%
GTATAACTCATCCCTGTAGT+TGG + chr6_3:97770584-97770603 None:intergenic 40.0%
CTACAGGGATGAGTTATACA+AGG - chr6_3:97770585-97770604 Msa0967670:intron 40.0%
! ACATATTGGTGACTCATCTG+TGG - chr6_3:97770915-97770934 Msa0967670:CDS 40.0%
! TATGAGAGGCACAATAAGGA+TGG + chr6_3:97770954-97770973 None:intergenic 40.0%
! GTATCGATATTGGATAGCGT+CGG - chr6_3:97771219-97771238 Msa0967670:intron 40.0%
!! TGAGGACTAGTTAGCAATTC+TGG + chr6_3:97771435-97771454 None:intergenic 40.0%
AAAAGAATCAGAGAAGCAGG+TGG - chr6_3:97771495-97771514 Msa0967670:intron 40.0%
ACTTACAAAGTGTGGAGAAG+AGG - chr6_3:97771610-97771629 Msa0967670:intron 40.0%
CTTACAAAGTGTGGAGAAGA+GGG - chr6_3:97771611-97771630 Msa0967670:intron 40.0%
AAGTCAGCTTAGACGGTAAA+AGG + chr6_3:97771685-97771704 None:intergenic 40.0%
! TTACCGTCTAAGCTGACTTT+CGG - chr6_3:97771686-97771705 Msa0967670:intron 40.0%
AGTTGTGACAACTTAGGATG+TGG - chr6_3:97771763-97771782 Msa0967670:intron 40.0%
CCACAACACTTGGTAAAACT+AGG + chr6_3:97771834-97771853 None:intergenic 40.0%
!!! CCTAGTTTTACCAAGTGTTG+TGG - chr6_3:97771831-97771850 Msa0967670:intron 40.0%
! TATAATGAAAGCACGGTGCA+TGG - chr6_3:97772441-97772460 Msa0967670:intron 40.0%
ATTACCAACGGTAGGATTTG+CGG - chr6_3:97772501-97772520 Msa0967670:intron 40.0%
! TCTACCTTTTGCGTGTGAAA+AGG - chr6_3:97772684-97772703 Msa0967670:intron 40.0%
GGACAGAAAAGTTCATTTCC+CGG - chr6_3:97772738-97772757 Msa0967670:CDS 40.0%
AGAAAAGTTCATTTCCCGGT+AGG - chr6_3:97772742-97772761 Msa0967670:CDS 40.0%
GTCATCTTTGAACTGTACAC+TGG + chr6_3:97772873-97772892 None:intergenic 40.0%
CATGTAATCCCATAAGCCAT+CGG + chr6_3:97772966-97772985 None:intergenic 40.0%
! GGGTAGCAGAAATGATTTTG+GGG + chr6_3:97773008-97773027 None:intergenic 40.0%
CTGCGGAACCAAAAAAAGAA+GGG + chr6_3:97773028-97773047 None:intergenic 40.0%
TCTGCGGAACCAAAAAAAGA+AGG + chr6_3:97773029-97773048 None:intergenic 40.0%
CAAGGATAGCAAGATTGTGA+AGG + chr6_3:97773058-97773077 None:intergenic 40.0%
! AGACGCAGTTTCTTTTGTGA+GGG - chr6_3:97773084-97773103 Msa0967670:intron 40.0%
AGGGATCAACTACGAAAACA+TGG - chr6_3:97773103-97773122 Msa0967670:intron 40.0%
ACGAAAACATGGAAACACAC+AGG - chr6_3:97773114-97773133 Msa0967670:intron 40.0%
TACCAAAACGACTTGTGCAA+GGG + chr6_3:97773244-97773263 None:intergenic 40.0%
TTACCAAAACGACTTGTGCA+AGG + chr6_3:97773245-97773264 None:intergenic 40.0%
TCCATTATGATACACTCTGC+AGG - chr6_3:97773318-97773337 Msa0967670:intron 40.0%
TCCTGCAGAGTGTATCATAA+TGG + chr6_3:97773322-97773341 None:intergenic 40.0%
TGACATTATCCTGTGTACGT+TGG + chr6_3:97773345-97773364 None:intergenic 40.0%
! AGAGCATTAATGTGGTCCAA+AGG - chr6_3:97773550-97773569 Msa0967670:intron 40.0%
AATAGACACAATTCCAACGG+TGG + chr6_3:97773794-97773813 None:intergenic 40.0%
ACCGTTGGAATTGTGTCTAT+TGG - chr6_3:97773793-97773812 Msa0967670:CDS 40.0%
! TGGAAGAGGTGGAGTGTTTA+TGG + chr6_3:97769800-97769819 None:intergenic 45.0%
! TGAAGGTGCATCAATGGCTA+TGG + chr6_3:97769886-97769905 None:intergenic 45.0%
GGAGAGTCTGTCAATGATGA+AGG + chr6_3:97769903-97769922 None:intergenic 45.0%
CAGACTCTCCTCCTATTAGA+TGG - chr6_3:97769913-97769932 Msa0967670:intron 45.0%
GACTCTCCTCCTATTAGATG+GGG - chr6_3:97769915-97769934 Msa0967670:intron 45.0%
ACTGATCCCCATCTAATAGG+AGG + chr6_3:97769924-97769943 None:intergenic 45.0%
ATGGGGATCAGTATCTTTGC+AGG - chr6_3:97769932-97769951 Msa0967670:intron 45.0%
TGGGGATCAGTATCTTTGCA+GGG - chr6_3:97769933-97769952 Msa0967670:intron 45.0%
CTTAGGCCAAATAGTCTCCA+AGG - chr6_3:97769987-97770006 Msa0967670:intron 45.0%
TCTCCAAGGTTTCTCTTTCG+CGG - chr6_3:97770001-97770020 Msa0967670:intron 45.0%
ATAGCTAAGACCTGAGGAAC+TGG + chr6_3:97770061-97770080 None:intergenic 45.0%
TGTGCCAAATGCCAACTACA+GGG - chr6_3:97770570-97770589 Msa0967670:intron 45.0%
TCATCCCTGTAGTTGGCATT+TGG + chr6_3:97770577-97770596 None:intergenic 45.0%
GAGTGTCTTGAAGCTCTACA+AGG - chr6_3:97770607-97770626 Msa0967670:intron 45.0%
TGTCTTGAAGCTCTACAAGG+TGG - chr6_3:97770610-97770629 Msa0967670:intron 45.0%
GTCTTGAAGCTCTACAAGGT+GGG - chr6_3:97770611-97770630 Msa0967670:intron 45.0%
AAGGCTTGAAATGAAAGGGG+AGG - chr6_3:97770828-97770847 Msa0967670:intron 45.0%
ACAGCAACTGCCATATTCCT+AGG - chr6_3:97770871-97770890 Msa0967670:intron 45.0%
AATCAGAGAAGCAGGTGGAT+GGG - chr6_3:97771500-97771519 Msa0967670:intron 45.0%
AGTGTGGAGAAGAGGGTTAT+TGG - chr6_3:97771618-97771637 Msa0967670:intron 45.0%
CGGAGACATTTGATGTGTTG+GGG + chr6_3:97771659-97771678 None:intergenic 45.0%
GCGGAGACATTTGATGTGTT+GGG + chr6_3:97771660-97771679 None:intergenic 45.0%
TGCGGAGACATTTGATGTGT+TGG + chr6_3:97771661-97771680 None:intergenic 45.0%
GCTTAGACGGTAAAAGGTTG+CGG + chr6_3:97771679-97771698 None:intergenic 45.0%
! TACCGTCTAAGCTGACTTTC+GGG - chr6_3:97771687-97771706 Msa0967670:intron 45.0%
CTATCACCTGACAGGCTAAA+GGG + chr6_3:97771943-97771962 None:intergenic 45.0%
GGTCCAAAATTGCACCATCA+AGG + chr6_3:97772391-97772410 None:intergenic 45.0%
ATTGCTGTATCCCGTGCATT+TGG - chr6_3:97772528-97772547 Msa0967670:intron 45.0%
TTGCTGTATCCCGTGCATTT+GGG - chr6_3:97772529-97772548 Msa0967670:intron 45.0%
! GCATCCTTTTCACACGCAAA+AGG + chr6_3:97772691-97772710 None:intergenic 45.0%
GATGCTACAAAAAGGAGCTC+AGG - chr6_3:97772706-97772725 Msa0967670:CDS 45.0%
CTACAAAAAGGAGCTCAGGA+AGG - chr6_3:97772710-97772729 Msa0967670:CDS 45.0%
TGTTAGCATCCGATGGCTTA+TGG - chr6_3:97772954-97772973 Msa0967670:CDS 45.0%
GTTAGCATCCGATGGCTTAT+GGG - chr6_3:97772955-97772974 Msa0967670:CDS 45.0%
GCTTATGGGATTACATGAGC+AGG - chr6_3:97772969-97772988 Msa0967670:CDS 45.0%
! CAGACGCAGTTTCTTTTGTG+AGG - chr6_3:97773083-97773102 Msa0967670:intron 45.0%
TATTATTGCGGCTGCTTCGA+TGG + chr6_3:97773187-97773206 None:intergenic 45.0%
! ATCCCTTGCACAAGTCGTTT+TGG - chr6_3:97773239-97773258 Msa0967670:intron 45.0%
! GTGACATTTTGTTGCTGCAG+TGG + chr6_3:97773748-97773767 None:intergenic 45.0%
TGGCTATGGCAGAACAGCAA+CGG + chr6_3:97769872-97769891 None:intergenic 50.0%
TTGCTTCTGTCCAGTTCCTC+AGG - chr6_3:97770048-97770067 Msa0967670:intron 50.0%
CTGTGCCAAATGCCAACTAC+AGG - chr6_3:97770569-97770588 Msa0967670:intron 50.0%
GCTTGAAATGAAAGGGGAGG+AGG - chr6_3:97770831-97770850 Msa0967670:intron 50.0%
TGAAATGAAAGGGGAGGAGG+AGG - chr6_3:97770834-97770853 Msa0967670:intron 50.0%
ACTAGTCCTCACCGTCCATA+TGG - chr6_3:97771444-97771463 Msa0967670:intron 50.0%
CTAGTCCTCACCGTCCATAT+GGG - chr6_3:97771445-97771464 Msa0967670:intron 50.0%
CCTTGTTGCTCCCATATGGA+CGG + chr6_3:97771458-97771477 None:intergenic 50.0%
GAATCAGAGAAGCAGGTGGA+TGG - chr6_3:97771499-97771518 Msa0967670:intron 50.0%
ACATTTGATGTGTTGGGGCG+TGG + chr6_3:97771654-97771673 None:intergenic 50.0%
GTCCCGAAAGTCAGCTTAGA+CGG + chr6_3:97771692-97771711 None:intergenic 50.0%
! TAAGCTGACTTTCGGGACTC+TGG - chr6_3:97771694-97771713 Msa0967670:intron 50.0%
CCTATCACCTGACAGGCTAA+AGG + chr6_3:97771944-97771963 None:intergenic 50.0%
CCTTTAGCCTGTCAGGTGAT+AGG - chr6_3:97771941-97771960 Msa0967670:intron 50.0%
ATCTCCGCAAATCCTACCGT+TGG + chr6_3:97772508-97772527 None:intergenic 50.0%
TGCTGTATCCCGTGCATTTG+GGG - chr6_3:97772530-97772549 Msa0967670:intron 50.0%
ATCGCATGTCCCCAAATGCA+CGG + chr6_3:97772542-97772561 None:intergenic 50.0%
AAGGAGCTCAGGAAGGAAGA+TGG - chr6_3:97772717-97772736 Msa0967670:CDS 50.0%
!! CATGTTGCACAGAAGTGCTC+TGG - chr6_3:97773605-97773624 Msa0967670:CDS 50.0%
CACAATTCCAACGGTGGCTA+GGG + chr6_3:97773788-97773807 None:intergenic 50.0%
ACACAATTCCAACGGTGGCT+AGG + chr6_3:97773789-97773808 None:intergenic 50.0%
GCAGGGTCCACGTGATGAAA+TGG - chr6_3:97769950-97769969 Msa0967670:intron 55.0%
TCTTTCGCGGCCGTGTTTGA+TGG - chr6_3:97770014-97770033 Msa0967670:intron 55.0%
CCCTCCATGGCCATCAAACA+CGG + chr6_3:97770027-97770046 None:intergenic 55.0%
! CCGTGTTTGATGGCCATGGA+GGG - chr6_3:97770024-97770043 Msa0967670:intron 55.0%
GGACAGAAGCAAACCCTCCA+TGG + chr6_3:97770040-97770059 None:intergenic 55.0%
TTGCTCCCATATGGACGGTG+AGG + chr6_3:97771453-97771472 None:intergenic 55.0%
CCGTCCATATGGGAGCAACA+AGG - chr6_3:97771455-97771474 Msa0967670:intron 55.0%
!! GAGGCCTTGTTGCTCCCATA+TGG + chr6_3:97771462-97771481 None:intergenic 55.0%
TGGTCTCCCTTTAGCCTGTC+AGG - chr6_3:97771934-97771953 Msa0967670:intron 55.0%
TCGCATGTCCCCAAATGCAC+GGG + chr6_3:97772541-97772560 None:intergenic 55.0%
GAAACTGCGTCTGAGCTGCA+AGG + chr6_3:97773076-97773095 None:intergenic 55.0%
TCTGCAGGACCAACGTACAC+AGG - chr6_3:97773333-97773352 Msa0967670:intron 55.0%
GTGCTTGCAGGAAGACAGAC+TGG - chr6_3:97773713-97773732 Msa0967670:CDS 55.0%
CGGCCGCGAAAGAGAAACCT+TGG + chr6_3:97770007-97770026 None:intergenic 60.0%
! GCCGTGTTTGATGGCCATGG+AGG - chr6_3:97770023-97770042 Msa0967670:intron 60.0%
GGGGAGGAGGAGGATGAATC+AGG - chr6_3:97770844-97770863 Msa0967670:intron 60.0%
TTCCAACGGTGGCTAGGGCT+TGG + chr6_3:97773783-97773802 None:intergenic 60.0%
! GCGGCCGTGTTTGATGGCCA+TGG - chr6_3:97770020-97770039 Msa0967670:intron 65.0%
GTCCAAGCCCTAGCCACCGT+TGG - chr6_3:97773778-97773797 Msa0967670:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 97769592 97774045 97769592 ID=Msa0967670;Name=Msa0967670
chr6_3 mRNA 97769592 97774045 97769592 ID=Msa0967670-mRNA-1;Parent=Msa0967670;Name=Msa0967670-mRNA-1;_AED=0.11;_eAED=0.11;_QI=158|0.9|0.90|1|1|1|11|199|400
chr6_3 exon 97769592 97769914 97769592 ID=Msa0967670-mRNA-1:exon:18199;Parent=Msa0967670-mRNA-1
chr6_3 exon 97770251 97770297 97770251 ID=Msa0967670-mRNA-1:exon:18198;Parent=Msa0967670-mRNA-1
chr6_3 exon 97770377 97770409 97770377 ID=Msa0967670-mRNA-1:exon:18197;Parent=Msa0967670-mRNA-1
chr6_3 exon 97770502 97770556 97770502 ID=Msa0967670-mRNA-1:exon:18196;Parent=Msa0967670-mRNA-1
chr6_3 exon 97770647 97770763 97770647 ID=Msa0967670-mRNA-1:exon:18195;Parent=Msa0967670-mRNA-1
chr6_3 exon 97770878 97770958 97770878 ID=Msa0967670-mRNA-1:exon:18194;Parent=Msa0967670-mRNA-1
chr6_3 exon 97771057 97771136 97771057 ID=Msa0967670-mRNA-1:exon:18193;Parent=Msa0967670-mRNA-1
chr6_3 exon 97772116 97772226 97772116 ID=Msa0967670-mRNA-1:exon:18192;Parent=Msa0967670-mRNA-1
chr6_3 exon 97772701 97772806 97772701 ID=Msa0967670-mRNA-1:exon:18191;Parent=Msa0967670-mRNA-1
chr6_3 exon 97772918 97773046 97772918 ID=Msa0967670-mRNA-1:exon:18190;Parent=Msa0967670-mRNA-1
chr6_3 exon 97773568 97774045 97773568 ID=Msa0967670-mRNA-1:exon:18189;Parent=Msa0967670-mRNA-1
chr6_3 five_prime_UTR 97773888 97774045 97773888 ID=Msa0967670-mRNA-1:five_prime_utr;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97773568 97773887 97773568 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97772918 97773046 97772918 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97772701 97772806 97772701 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97772116 97772226 97772116 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97771057 97771136 97771057 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97770878 97770958 97770878 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97770647 97770763 97770647 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97770502 97770556 97770502 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97770377 97770409 97770377 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97770251 97770297 97770251 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 CDS 97769791 97769914 97769791 ID=Msa0967670-mRNA-1:cds;Parent=Msa0967670-mRNA-1
chr6_3 three_prime_UTR 97769592 97769790 97769592 ID=Msa0967670-mRNA-1:three_prime_utr;Parent=Msa0967670-mRNA-1
Gene Sequence

>Msa0967670

ATGGCGTTATCAACACCACATATACAAAGATTCCTCTTAACTAAGCTCCATAAACACTCCACCTCTTCCAAATACATAAACACAAACAGAAACAAAAACAGATTCATCAGAAGAAGAAACCGTTGCTGTTCTGCCATAGCCATTGATGCACCTTCATCATTGACAGACTCTCCTCCTATTAGATGGGGATCAGTATCTTTGCAGGGTCCACGTGATGAAATGGAAGATGATATTATTCTTAGGCCAAATAGTCTCCAAGGTTTCTCTTTCGCGGCCGTGTTTGATGGCCATGGAGGGTTTGCTTCTGTCCAGTTCCTCAGGGATGAGTTATACAAGGAGTGTCTTGAAGCTCTACAAGGTGGGTTACTATTGCTTGAGAAAGATTTCAAAGCTGTTGAAGAAGCATTAAAGCAGGCATTTGTTAAAGCTGACATGAGGTTGTTAAAATGGCTTGAAATGAAAGGGGAGGAGGAGGATGAATCAGGTGCTACAGCAACTGCCATATTCCTAGGAAATAATAAGCTGCTAATTTCACATATTGGTGACTCATCTGTGGTTTTATGCAGATCTGGAAAACCAGAATTGCTAACTAGTCCTCACCGTCCATATGGGAGCAACAAGGCCTCTCTTCAAGAAATCAAAAGAATCAGAGAAGCAGGTGGATGGATTACCAACGGTAGGATTTGCGGAGATATTGCTGTATCCCGTGCATTTGGGGACATGCGATTCAAAACAAAGAAGATCGATGTTATCTACCTTTTGCGTGTGAAAAGGATGCTACAAAAAGGAGCTCAGGAAGGAAGATGGACAGAAAAGTTCATTTCCCGTGTACAGTTCAAAGATGACTTGGTTGTTGCACGCCCTGATATTTATCAAGTAACTCTTGGCTCAGATGCAGAATTTATAGTGTTAGCATCCGATGGCTTATGGGATTACATGAGCAGCTCAGACGCAGTTTCTTTTGTGAGGGATCAACTACGAAAACATGGAAACACACAGCTAGCTTGTGAATCCCTTGCACAAGTCGTTTTGGACCAACGTACACAGGATAATGTCAGCATTATAATTGCCGATCTAGGGAAGACAGACTGGAAGAGTGTACCACTGCAGCAACAAAATGTCACATTTGAGCTAGTCCAAGCCCTAGCCACCGTTGGAATTGTGTCTATTGGAATCTGGTTTTCATCACAGCTTTCTTTATAG
Protein sequence

>Msa0967670

MALSTPHIQRFLLTKLHKHSTSSKYINTNRNKNRFIRRRNRCCSAIAIDAPSSLTDSPPIRWGSVSLQGPRDEMEDDIILRPNSLQGFSFAAVFDGHGGFASVQFLRDELYKECLEALQGGLLLLEKDFKAVEEALKQAFVKADMRLLKWLEMKGEEEDESGATATAIFLGNNKLLISHIGDSSVVLCRSGKPELLTSPHRPYGSNKASLQEIKRIREAGGWITNGRICGDIAVSRAFGDMRFKTKKIDVIYLLRVKRMLQKGAQEGRWTEKFISRVQFKDDLVVARPDIYQVTLGSDAEFIVLASDGLWDYMSSSDAVSFVRDQLRKHGNTQLACESLAQVVLDQRTQDNVSIIIADLGKTDWKSVPLQQQNVTFELVQALATVGIVSIGIWFSSQLSL*