AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0115460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115460 MtrunA17_Chr1g0212421 98.361 122 2 0 1 122 1 122 3.53e-84 241
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115460 AT5G08535.4 60.526 114 35 2 10 122 12 116 3.88e-36 120
Msa0115460 AT5G08535.2 60.526 114 35 2 10 122 12 116 3.88e-36 120
Msa0115460 AT5G08535.3 60.526 114 35 2 10 122 12 116 3.88e-36 120
Msa0115460 AT5G08535.1 60.526 114 35 2 10 122 37 141 6.36e-36 120
Msa0115460 AT3G52350.1 57.391 115 40 3 9 122 74 180 1.71e-29 105

Find 50 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTCCAATATAGGGTTTC+AGG 0.095136 1_3:-34344950 Msa0115460:intron
CAACGTTTCTTCCATGACTA+TGG 0.275578 1_3:+34344996 None:intergenic
ACAATCAACTCTTCCAATAT+AGG 0.297658 1_3:-34344958 Msa0115460:CDS
TACCTGCTTATTGTCTCCTT+TGG 0.305621 1_3:+34344191 None:intergenic
AGAGTTCCCCGTATGGGTTT+TGG 0.312465 1_3:-34341138 Msa0115460:three_prime_UTR
GCGCTTTCTTTAGAGAGTTC+TGG 0.323271 1_3:-34341634 Msa0115460:CDS
CAATCAACTCTTCCAATATA+GGG 0.331331 1_3:-34344957 Msa0115460:CDS
AAGAATAATAAACGAGGTTT+GGG 0.354264 1_3:-34344264 Msa0115460:CDS
AAGAACCTGAAACCCTATAT+TGG 0.360282 1_3:+34344945 None:intergenic
CTGCTTATTGTCTCCTTTGG+AGG 0.363940 1_3:+34344194 None:intergenic
AAAGAATAATAAACGAGGTT+TGG 0.364649 1_3:-34344265 Msa0115460:CDS
TGGAAAGAAGGGACTGGTCT+TGG 0.385117 1_3:-34344800 Msa0115460:CDS
CCAGGATACTTACAGGATAT+AGG 0.394488 1_3:-34341483 Msa0115460:three_prime_UTR
CCTATATCCTGTAAGTATCC+TGG 0.407046 1_3:+34341483 None:intergenic
AAGCCAAAGCACTTTCTAAA+CGG 0.409716 1_3:-34341709 Msa0115460:CDS
CACCCGTTTAGAAAGTGCTT+TGG 0.422675 1_3:+34341706 None:intergenic
ATGTGTCAAATGTGCAAGTA+TGG 0.429149 1_3:-34341167 Msa0115460:three_prime_UTR
TTGGAGGAATCACCGTGATC+AGG 0.437824 1_3:+34344210 None:intergenic
AAGAAGCATGGTTGGAAAGA+AGG 0.444986 1_3:-34344812 Msa0115460:CDS
AGCCAAAGCACTTTCTAAAC+GGG 0.461909 1_3:-34341708 Msa0115460:CDS
AACTCATCCAAAACCCATAC+GGG 0.471803 1_3:+34341131 None:intergenic
TGAAAAGAAGATGCAGGAGA+AGG 0.481160 1_3:-34341666 Msa0115460:CDS
TGGTCTTGGAATCTCTGAGC+AGG 0.482435 1_3:-34344786 Msa0115460:intron
ACTAAGAAAGTGAATGCAAA+AGG 0.483023 1_3:+34341557 None:intergenic
CTATATCCTGTAAGTATCCT+GGG 0.489397 1_3:+34341484 None:intergenic
ATGATAGGCCATGTCCCTCC+TGG 0.498265 1_3:-34341335 Msa0115460:three_prime_UTR
AGCAGATATAGTGAAGAAGA+AGG 0.498955 1_3:-34344241 Msa0115460:CDS
ATCAGAGGGAAGGTAATGAT+AGG 0.500568 1_3:-34341350 Msa0115460:three_prime_UTR
TTCTTTACACGAGTTTCCAC+AGG 0.518432 1_3:+34344282 None:intergenic
TTCTTGAGAGTTCCCCGTAT+GGG 0.519570 1_3:-34341144 Msa0115460:three_prime_UTR
CAACTCATCCAAAACCCATA+CGG 0.535090 1_3:+34341130 None:intergenic
AGAAGCATGGTTGGAAAGAA+GGG 0.546784 1_3:-34344811 Msa0115460:CDS
AGAGATTGAAAAGAAGATGC+AGG 0.557295 1_3:-34341672 Msa0115460:CDS
GTTCTTGAGAGTTCCCCGTA+TGG 0.557993 1_3:-34341145 Msa0115460:three_prime_UTR
TTTCAAAGTCGTGTGCAAGT+TGG 0.562551 1_3:-34341523 Msa0115460:three_prime_UTR
CATAGTCATGGAAGAAACGT+TGG 0.564577 1_3:-34344995 Msa0115460:exon
TTTGTGTAAATCCATAGTCA+TGG 0.565979 1_3:-34345007 Msa0115460:exon
ATAGTCATGGAAGAAACGTT+GGG 0.566192 1_3:-34344994 Msa0115460:exon
CTCCAAAGGAGACAATAAGC+AGG 0.587624 1_3:-34344193 Msa0115460:intron
GGAAGTGTCTTTAGAGCTAG+TGG 0.595771 1_3:+34341247 None:intergenic
AAATGTATGAGAATTGATGA+TGG 0.601449 1_3:-34341606 Msa0115460:exon
CATGGTTGGAAAGAAGGGAC+TGG 0.614326 1_3:-34344806 Msa0115460:CDS
GATCACGGTGATTCCTCCAA+AGG 0.624641 1_3:-34344207 Msa0115460:CDS
ACAGGGTAGATTAGAACCTG+TGG 0.628498 1_3:-34344298 Msa0115460:intron
AATGTATGAGAATTGATGAT+GGG 0.631074 1_3:-34341605 Msa0115460:exon
AAGAATATATAAAATACATG+TGG 0.634241 1_3:+34341226 None:intergenic
GTGTGCAAGTTGGATCTCGA+AGG 0.648173 1_3:-34341513 Msa0115460:three_prime_UTR
AAGGTTGTGAAACCTGATCA+CGG 0.667061 1_3:-34344222 Msa0115460:CDS
CGTGTAAAGAATAATAAACG+AGG 0.690122 1_3:-34344270 Msa0115460:CDS
ACTCATCCAAAACCCATACG+GGG 0.741115 1_3:+34341132 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTATAAAAATAAAAA+AGG - chr1_3:34344005-34344024 Msa0115460:intron 0.0%
!!! TTTTTAAAAAAAAAAAAAAA+TGG - chr1_3:34344928-34344947 Msa0115460:intron 0.0%
!!! TTAAAAATATATTTAAAGTG+GGG - chr1_3:34343307-34343326 Msa0115460:intron 10.0%
!!! TATAAAAATAAAAAAGGTAT+AGG - chr1_3:34344011-34344030 Msa0115460:intron 10.0%
!!! ATAAAAATAAAAAAGGTATA+GGG - chr1_3:34344012-34344031 Msa0115460:intron 10.0%
!!! TTTTTTTTTTTTTAAAAACC+AGG + chr1_3:34344926-34344945 None:intergenic 10.0%
!! ACTAAAAATTGATATGAAAT+TGG + chr1_3:34341109-34341128 None:intergenic 15.0%
!! AAAAAAAGAAAAAGAAAGAA+GGG + chr1_3:34341330-34341349 None:intergenic 15.0%
!!! TGTAATTGTAAATGTAATTT+TGG - chr1_3:34341374-34341393 Msa0115460:three_prime_UTR 15.0%
!! AATAGAATTATGCAAATAAT+AGG - chr1_3:34342557-34342576 Msa0115460:intron 15.0%
!! ATAGAATTATGCAAATAATA+GGG - chr1_3:34342558-34342577 Msa0115460:intron 15.0%
!! ATATGCATTCTTAAAAAAAA+TGG - chr1_3:34342739-34342758 Msa0115460:intron 15.0%
!! ATTCTTAAAAAAAATGGAAA+AGG - chr1_3:34342745-34342764 Msa0115460:intron 15.0%
!! TTCTTAAAAAAAATGGAAAA+GGG - chr1_3:34342746-34342765 Msa0115460:intron 15.0%
!!! TTTTCTTATTTGAGTTTAAA+GGG + chr1_3:34343396-34343415 None:intergenic 15.0%
!! AATTATTAGAAAGAAAATTG+AGG - chr1_3:34343660-34343679 Msa0115460:intron 15.0%
!! AAACTTCAAAAATAAGAAAT+AGG - chr1_3:34344276-34344295 Msa0115460:CDS 15.0%
!! GAATCGAAAAAAAAAAAAAA+AGG + chr1_3:34344843-34344862 None:intergenic 15.0%
!! AAGAATATATAAAATACATG+TGG + chr1_3:34345017-34345036 None:intergenic 15.0%
!! GAAAAAAAGAAAAAGAAAGA+AGG + chr1_3:34341331-34341350 None:intergenic 20.0%
!! TGTGCGATTAAATTAAATTA+GGG + chr1_3:34341683-34341702 None:intergenic 20.0%
!! ATGTGCGATTAAATTAAATT+AGG + chr1_3:34341684-34341703 None:intergenic 20.0%
!!! TAAATTAGTATGAGTATTGT+AGG - chr1_3:34341751-34341770 Msa0115460:intron 20.0%
!!! TCTGATACACAATTTTATTT+TGG - chr1_3:34341917-34341936 Msa0115460:intron 20.0%
!!! ACAATTTTATTTTGGTAACA+GGG - chr1_3:34341925-34341944 Msa0115460:intron 20.0%
!! TAACAATTTACAATAGTAGA+AGG - chr1_3:34342119-34342138 Msa0115460:intron 20.0%
!! AACAATTTACAATAGTAGAA+GGG - chr1_3:34342120-34342139 Msa0115460:intron 20.0%
!! AACTACATAACTATGTAAAA+CGG - chr1_3:34342144-34342163 Msa0115460:intron 20.0%
!!! TTATTTTATTGAATTTGGTG+AGG - chr1_3:34342287-34342306 Msa0115460:intron 20.0%
!! ATCATTTGTAATCAAACAAA+TGG - chr1_3:34342493-34342512 Msa0115460:intron 20.0%
!! TCTTAAAAAAAATGGAAAAG+GGG - chr1_3:34342747-34342766 Msa0115460:intron 20.0%
!! ACCTCAAAAATATATATGAA+GGG - chr1_3:34342832-34342851 Msa0115460:intron 20.0%
!! ATTCATTTCTTAGATATGTT+TGG + chr1_3:34342987-34343006 None:intergenic 20.0%
!!! CATGTTTTTATGAATGATAT+TGG - chr1_3:34343015-34343034 Msa0115460:intron 20.0%
!!! GTTTTCTTATTTGAGTTTAA+AGG + chr1_3:34343397-34343416 None:intergenic 20.0%
!! GAAGTAAAGTGAATTAAAAA+AGG - chr1_3:34343505-34343524 Msa0115460:intron 20.0%
!! TCTAAATCATTCTTAATGTT+AGG + chr1_3:34343833-34343852 None:intergenic 20.0%
!! TAACATTAAGAATGATTTAG+AGG - chr1_3:34343832-34343851 Msa0115460:intron 20.0%
!! TAATAAATGACATAACTGTA+AGG - chr1_3:34343876-34343895 Msa0115460:intron 20.0%
!! AGAATATCTTAAAATAAGCA+CGG - chr1_3:34343973-34343992 Msa0115460:intron 20.0%
!!! TTTTTTTCAGAGAGAAAAAA+GGG + chr1_3:34344122-34344141 None:intergenic 20.0%
!! CTCTTCAAATTAAAAAGAAA+TGG - chr1_3:34344250-34344269 Msa0115460:CDS 20.0%
!! TCATATTTAACAATAATGCA+AGG - chr1_3:34344383-34344402 Msa0115460:intron 20.0%
!! CATATTTAACAATAATGCAA+GGG - chr1_3:34344384-34344403 Msa0115460:intron 20.0%
!!! AATTTTTTTTTGCAAACAGA+AGG - chr1_3:34344780-34344799 Msa0115460:intron 20.0%
!! ACTATTTACAGATGAAAAAA+GGG + chr1_3:34344817-34344836 None:intergenic 20.0%
!! AGAAATATACAAAAATCAGA+GGG - chr1_3:34344876-34344895 Msa0115460:intron 20.0%
!!! TTTTTTTTTAAAAACCAGGA+GGG + chr1_3:34344922-34344941 None:intergenic 20.0%
!!! TTTTTTTTTTAAAAACCAGG+AGG + chr1_3:34344923-34344942 None:intergenic 20.0%
!! GTAAATGTAATTTTGGATGA+TGG - chr1_3:34341381-34341400 Msa0115460:three_prime_UTR 25.0%
! TACAAATTTAATGCCCTTAA+AGG + chr1_3:34341624-34341643 None:intergenic 25.0%
!! TTTGTGTCAAATTAGTTCTT+AGG - chr1_3:34341645-34341664 Msa0115460:CDS 25.0%
!! TTTAATTTAATCGCACATTC+AGG - chr1_3:34341686-34341705 Msa0115460:CDS 25.0%
! TTAATTTAATCGCACATTCA+GGG - chr1_3:34341687-34341706 Msa0115460:CDS 25.0%
! ACTCATACTAATTTAACTCA+AGG + chr1_3:34341746-34341765 None:intergenic 25.0%
! TACAAGAGAAATACTGAAAT+AGG + chr1_3:34341861-34341880 None:intergenic 25.0%
!!! TTGTATTCTGAAACAGTTTT+AGG - chr1_3:34341876-34341895 Msa0115460:intron 25.0%
!!! TGTATTCTGAAACAGTTTTA+GGG - chr1_3:34341877-34341896 Msa0115460:intron 25.0%
!!! CACAATTTTATTTTGGTAAC+AGG - chr1_3:34341924-34341943 Msa0115460:intron 25.0%
! AAAGAATAATAAACGAGGTT+TGG - chr1_3:34341975-34341994 Msa0115460:intron 25.0%
! AAGAATAATAAACGAGGTTT+GGG - chr1_3:34341976-34341995 Msa0115460:intron 25.0%
! ACCTAAAATAAGAAAACCTT+CGG + chr1_3:34342211-34342230 None:intergenic 25.0%
! TGGTATAGTACTTAGTAATT+AGG - chr1_3:34342317-34342336 Msa0115460:intron 25.0%
! AGAAGTTGAGTAATTAATGA+AGG - chr1_3:34342346-34342365 Msa0115460:intron 25.0%
! TGTCATGGAATTTAGTAATT+AGG - chr1_3:34342404-34342423 Msa0115460:intron 25.0%
! AGATATACTTTGTTGACATA+TGG - chr1_3:34342460-34342479 Msa0115460:intron 25.0%
!! ATATCTATCTCTAAGAACAA+AGG - chr1_3:34342381-34342400 Msa0115460:intron 25.0%
! GAGAATAACTATAGAAATAC+AGG - chr1_3:34342681-34342700 Msa0115460:intron 25.0%
! GACCTCAAAAATATATATGA+AGG - chr1_3:34342831-34342850 Msa0115460:intron 25.0%
!!! ACTGTATTTTGCTTTTACTT+TGG + chr1_3:34343131-34343150 None:intergenic 25.0%
! TGAAAATAAGAGAACCTAAA+GGG - chr1_3:34343198-34343217 Msa0115460:intron 25.0%
!!! TGAAAACAAATCTTTTTGAG+AGG + chr1_3:34343538-34343557 None:intergenic 25.0%
! TTCTACCATCTCTATTATAA+AGG + chr1_3:34343689-34343708 None:intergenic 25.0%
!!! GTGCTTATTTTAAGATATTC+TGG + chr1_3:34343974-34343993 None:intergenic 25.0%
!! CTTTTATATTGAACAACTCT+AGG - chr1_3:34344039-34344058 Msa0115460:intron 25.0%
!!! TTTTTTCAGAGAGAAAAAAG+GGG + chr1_3:34344121-34344140 None:intergenic 25.0%
!!! GTTTTTTTCAGAGAGAAAAA+AGG + chr1_3:34344123-34344142 None:intergenic 25.0%
!!! CTTTTTAATTTGAAGAGAAG+AGG + chr1_3:34344247-34344266 None:intergenic 25.0%
! TCAAAAATAAGAAATAGGAG+AGG - chr1_3:34344281-34344300 Msa0115460:CDS 25.0%
! ATAATATGATATCCGTGAAA+GGG + chr1_3:34344416-34344435 None:intergenic 25.0%
! TATAATATGATATCCGTGAA+AGG + chr1_3:34344417-34344436 None:intergenic 25.0%
!! TTTTCAGTCATTTGGATTTT+TGG - chr1_3:34344465-34344484 Msa0115460:intron 25.0%
!! ATCAATTCTCATACATTTTC+AGG + chr1_3:34344632-34344651 None:intergenic 25.0%
! AAATGTATGAGAATTGATGA+TGG - chr1_3:34344634-34344653 Msa0115460:intron 25.0%
!! AATGTATGAGAATTGATGAT+GGG - chr1_3:34344635-34344654 Msa0115460:intron 25.0%
! GACTATTTACAGATGAAAAA+AGG + chr1_3:34344818-34344837 None:intergenic 25.0%
! GAGAAATATACAAAAATCAG+AGG - chr1_3:34344875-34344894 Msa0115460:intron 25.0%
!! ATATTGTGTTCATTCTTTTC+AGG - chr1_3:34344963-34344982 Msa0115460:CDS 25.0%
! GTTTTACGATGTATCTTCAA+TGG - chr1_3:34341167-34341186 Msa0115460:three_prime_UTR 30.0%
TTTGTGTAAATCCATAGTCA+TGG - chr1_3:34341233-34341252 Msa0115460:three_prime_UTR 30.0%
ACAATCAACTCTTCCAATAT+AGG - chr1_3:34341282-34341301 Msa0115460:three_prime_UTR 30.0%
CAATCAACTCTTCCAATATA+GGG - chr1_3:34341283-34341302 Msa0115460:three_prime_UTR 30.0%
! GAGTATTGTAGGTTAATTGA+TGG - chr1_3:34341762-34341781 Msa0115460:intron 30.0%
TGTTTATATCAGTTCTCCTT+AGG + chr1_3:34341792-34341811 None:intergenic 30.0%
!!! GTATTCTGAAACAGTTTTAG+GGG - chr1_3:34341878-34341897 Msa0115460:intron 30.0%
!!! GTGTATCAGAATTTTTTGCA+AGG + chr1_3:34341907-34341926 None:intergenic 30.0%
CGTGTAAAGAATAATAAACG+AGG - chr1_3:34341970-34341989 Msa0115460:intron 30.0%
!! AAAGTTTGCTTTTGTTCTCA+AGG - chr1_3:34342080-34342099 Msa0115460:intron 30.0%
!! AAGTTTGCTTTTGTTCTCAA+GGG - chr1_3:34342081-34342100 Msa0115460:intron 30.0%
!! CTGAGTTTTTCATTTGGAAT+TGG - chr1_3:34342169-34342188 Msa0115460:intron 30.0%
!! TTTTATTGAATTTGGTGAGG+TGG - chr1_3:34342290-34342309 Msa0115460:intron 30.0%
! TTTATTGAATTTGGTGAGGT+GGG - chr1_3:34342291-34342310 Msa0115460:intron 30.0%
GTAGATTTATAATACGACTC+AGG + chr1_3:34342591-34342610 None:intergenic 30.0%
AGTCGTATTATAAATCTACG+AGG - chr1_3:34342592-34342611 Msa0115460:intron 30.0%
GTCGTATTATAAATCTACGA+GGG - chr1_3:34342593-34342612 Msa0115460:intron 30.0%
! ACTATAGAAATACAGGTTGA+TGG - chr1_3:34342688-34342707 Msa0115460:intron 30.0%
!! GCCCTTCATATATATTTTTG+AGG + chr1_3:34342836-34342855 None:intergenic 30.0%
TCGAACTACAAAACACTTTA+CGG - chr1_3:34342879-34342898 Msa0115460:intron 30.0%
GTGTTTGTAGTTTCTAAACT+TGG + chr1_3:34343163-34343182 None:intergenic 30.0%
ACAAACACATCATAACATAG+AGG - chr1_3:34343175-34343194 Msa0115460:intron 30.0%
GTGAAAATAAGAGAACCTAA+AGG - chr1_3:34343197-34343216 Msa0115460:intron 30.0%
GAAAATAAGAGAACCTAAAG+GGG - chr1_3:34343199-34343218 Msa0115460:intron 30.0%
GTTTGTCATACAAAGTGATA+GGG - chr1_3:34343453-34343472 Msa0115460:intron 30.0%
TTTGTCATACAAAGTGATAG+GGG - chr1_3:34343454-34343473 Msa0115460:intron 30.0%
! ATAAAGATGAGTTGAATGAG+TGG - chr1_3:34343600-34343619 Msa0115460:intron 30.0%
ATCCAATTTACTCTTCTTAC+AGG + chr1_3:34343759-34343778 None:intergenic 30.0%
TGTAAGAAGAGTAAATTGGA+TGG - chr1_3:34343758-34343777 Msa0115460:intron 30.0%
AGTCCAATTGTTATAGACAA+AGG - chr1_3:34343786-34343805 Msa0115460:intron 30.0%
GTCCAATTGTTATAGACAAA+GGG - chr1_3:34343787-34343806 Msa0115460:intron 30.0%
ATTGAACAACTCTAGGTAAT+TGG - chr1_3:34344046-34344065 Msa0115460:intron 30.0%
TTTCACATTTGTCATTCTGA+TGG - chr1_3:34344151-34344170 Msa0115460:intron 30.0%
TAATATGATATCCGTGAAAG+GGG + chr1_3:34344415-34344434 None:intergenic 30.0%
TGACTGAAAACGAAAAACAA+CGG + chr1_3:34344455-34344474 None:intergenic 30.0%
! GTTTTTCGTTTTCAGTCATT+TGG - chr1_3:34344457-34344476 Msa0115460:intron 30.0%
! GATTGACTTCAATTGATTTC+AGG - chr1_3:34344490-34344509 Msa0115460:intron 30.0%
ACTAAGAAAGTGAATGCAAA+AGG + chr1_3:34344686-34344705 None:intergenic 30.0%
! TTTTCGATTCTTCTGTACAA+TGG - chr1_3:34344853-34344872 Msa0115460:intron 30.0%
ATATACAAAAATCAGAGGGA+AGG - chr1_3:34344880-34344899 Msa0115460:intron 30.0%
! CTGTTCAACGATTCCTTTTA+AGG - chr1_3:34341136-34341155 Msa0115460:three_prime_UTR 35.0%
!!! GATTCCTTTTAAGGCTTCAA+GGG - chr1_3:34341145-34341164 Msa0115460:three_prime_UTR 35.0%
AAAACCCTTGAAGCCTTAAA+AGG + chr1_3:34341152-34341171 None:intergenic 35.0%
ACGATGTATCTTCAATGGTT+TGG - chr1_3:34341172-34341191 Msa0115460:three_prime_UTR 35.0%
!! GTATCTTCAATGGTTTGGTT+TGG - chr1_3:34341177-34341196 Msa0115460:three_prime_UTR 35.0%
ATAGTCATGGAAGAAACGTT+GGG - chr1_3:34341246-34341265 Msa0115460:three_prime_UTR 35.0%
AAGAACCTGAAACCCTATAT+TGG + chr1_3:34341298-34341317 None:intergenic 35.0%
TAGTAATATCATGTCTGTGC+CGG - chr1_3:34341529-34341548 Msa0115460:three_prime_UTR 35.0%
! TCAAATTAGTTCTTAGGTCC+AGG - chr1_3:34341651-34341670 Msa0115460:CDS 35.0%
CTACTAAATATGCAATTCCC+TGG + chr1_3:34341723-34341742 None:intergenic 35.0%
AGCAGATATAGTGAAGAAGA+AGG - chr1_3:34341999-34342018 Msa0115460:intron 35.0%
AAAATAAGAAAACCTTCGGC+AGG + chr1_3:34342207-34342226 None:intergenic 35.0%
!!! GCCGAAGGTTTTCTTATTTT+AGG - chr1_3:34342207-34342226 Msa0115460:intron 35.0%
! TTATTGAATTTGGTGAGGTG+GGG - chr1_3:34342292-34342311 Msa0115460:intron 35.0%
! CTCTAAGAACAAAGTTGTCA+TGG - chr1_3:34342389-34342408 Msa0115460:intron 35.0%
AAACCAGCTTTGCTTATGTT+TGG - chr1_3:34342658-34342677 Msa0115460:intron 35.0%
AACCAGCTTTGCTTATGTTT+GGG - chr1_3:34342659-34342678 Msa0115460:intron 35.0%
! GAGAATCTAGAGAGGTTTTA+TGG - chr1_3:34342777-34342796 Msa0115460:intron 35.0%
!! ATCTAGAGAGGTTTTATGGA+AGG - chr1_3:34342781-34342800 Msa0115460:intron 35.0%
AAACACTTTACGGTAACCAT+AGG - chr1_3:34342889-34342908 Msa0115460:intron 35.0%
TGACATTTAATGCACACCTA+TGG + chr1_3:34342908-34342927 None:intergenic 35.0%
CTATCTATCTCTATCTCTCT+TGG + chr1_3:34343054-34343073 None:intergenic 35.0%
GCAATATTGGGAGTTCTTAT+TGG + chr1_3:34343262-34343281 None:intergenic 35.0%
TTCTCACATTTGTAGTACCT+TGG - chr1_3:34343430-34343449 Msa0115460:intron 35.0%
TCTCACATTTGTAGTACCTT+GGG - chr1_3:34343431-34343450 Msa0115460:intron 35.0%
GGTTTGTCATACAAAGTGAT+AGG - chr1_3:34343452-34343471 Msa0115460:intron 35.0%
GGTAGCCTTTATAATAGAGA+TGG - chr1_3:34343681-34343700 Msa0115460:intron 35.0%
CACCTGTAAGAAGAGTAAAT+TGG - chr1_3:34343754-34343773 Msa0115460:intron 35.0%
CTCCCTTTGTCTATAACAAT+TGG + chr1_3:34343792-34343811 None:intergenic 35.0%
! GTTAGGTCTATCATTTTCCT+TGG + chr1_3:34343816-34343835 None:intergenic 35.0%
TTTGTCATTCTGATGGTACT+TGG - chr1_3:34344158-34344177 Msa0115460:intron 35.0%
TCATGTGTATGTTTCACTGA+AGG - chr1_3:34344199-34344218 Msa0115460:CDS 35.0%
! TGATTCAATACAGGGTACTA+GGG + chr1_3:34344352-34344371 None:intergenic 35.0%
! AAGCCAAAGCACTTTCTAAA+CGG - chr1_3:34344531-34344550 Msa0115460:intron 35.0%
AGAGATTGAAAAGAAGATGC+AGG - chr1_3:34344568-34344587 Msa0115460:intron 35.0%
CTATATCCTGTAAGTATCCT+GGG + chr1_3:34344759-34344778 None:intergenic 35.0%
ATGTGTCAAATGTGCAAGTA+TGG - chr1_3:34345073-34345092 Msa0115460:intron 35.0%
!!! CGATTCCTTTTAAGGCTTCA+AGG - chr1_3:34341144-34341163 Msa0115460:three_prime_UTR 40.0%
CAACGTTTCTTCCATGACTA+TGG + chr1_3:34341247-34341266 None:intergenic 40.0%
CATAGTCATGGAAGAAACGT+TGG - chr1_3:34341245-34341264 Msa0115460:three_prime_UTR 40.0%
CTCTTCCAATATAGGGTTTC+AGG - chr1_3:34341290-34341309 Msa0115460:three_prime_UTR 40.0%
!! AGCTTTTGAAGAAGCATGGT+TGG - chr1_3:34341420-34341439 Msa0115460:three_prime_UTR 40.0%
! AAGAAGCATGGTTGGAAAGA+AGG - chr1_3:34341428-34341447 Msa0115460:three_prime_UTR 40.0%
! AGAAGCATGGTTGGAAAGAA+GGG - chr1_3:34341429-34341448 Msa0115460:three_prime_UTR 40.0%
ATCCAGGAACTTCAAAAGCA+CGG + chr1_3:34341590-34341609 None:intergenic 40.0%
! ATGGCGGAAGTTACCTTTAA+GGG - chr1_3:34341608-34341627 Msa0115460:exon 40.0%
ATTAAATTAGGGCAGAGTCC+TGG + chr1_3:34341672-34341691 None:intergenic 40.0%
! ATTCAGGGACTAATTTTGCC+AGG - chr1_3:34341702-34341721 Msa0115460:CDS 40.0%
! TTCAGGGACTAATTTTGCCA+GGG - chr1_3:34341703-34341722 Msa0115460:CDS 40.0%
GTTAATTGATGGAACGCCTA+AGG - chr1_3:34341773-34341792 Msa0115460:intron 40.0%
TTCTTTACACGAGTTTCCAC+AGG + chr1_3:34341961-34341980 None:intergenic 40.0%
AAGGTTGTGAAACCTGATCA+CGG - chr1_3:34342018-34342037 Msa0115460:intron 40.0%
TACCTGCTTATTGTCTCCTT+TGG + chr1_3:34342052-34342071 None:intergenic 40.0%
! GCTTTTGTTCTCAAGGGTTT+TGG - chr1_3:34342087-34342106 Msa0115460:intron 40.0%
!! ACGGTGCTGAGTTTTTCATT+TGG - chr1_3:34342163-34342182 Msa0115460:intron 40.0%
! TTAGGTAGTACTAGCTTGTC+AGG - chr1_3:34342225-34342244 Msa0115460:intron 40.0%
! TATTGAATTTGGTGAGGTGG+GGG - chr1_3:34342293-34342312 Msa0115460:intron 40.0%
GCAAGATAGAGAATCTAGAG+AGG - chr1_3:34342769-34342788 Msa0115460:intron 40.0%
! TTGCGACAATCTTTAGAAGC+AGG - chr1_3:34343095-34343114 Msa0115460:intron 40.0%
AGTGCAGAAGTGGCAATATT+GGG + chr1_3:34343274-34343293 None:intergenic 40.0%
AAGTGCAGAAGTGGCAATAT+TGG + chr1_3:34343275-34343294 None:intergenic 40.0%
GTTTGTATGAAGTGCAGAAG+TGG + chr1_3:34343284-34343303 None:intergenic 40.0%
CACTTTGTATGACAAACCCA+AGG + chr1_3:34343450-34343469 None:intergenic 40.0%
! AATCCCGACTTATGGTGTTT+TGG - chr1_3:34343707-34343726 Msa0115460:intron 40.0%
! ATCCCGACTTATGGTGTTTT+GGG - chr1_3:34343708-34343727 Msa0115460:intron 40.0%
ATGCCCAAAACACCATAAGT+CGG + chr1_3:34343714-34343733 None:intergenic 40.0%
TTATAGACAAAGGGAGACCA+AGG - chr1_3:34343796-34343815 Msa0115460:intron 40.0%
GAGTCTGCTACAAAGATCAA+TGG + chr1_3:34343905-34343924 None:intergenic 40.0%
TTGTAGCAGACTCTACATAG+TGG - chr1_3:34343912-34343931 Msa0115460:intron 40.0%
TGTAGCAGACTCTACATAGT+GGG - chr1_3:34343913-34343932 Msa0115460:intron 40.0%
! AGACTCTACATAGTGGGAAA+AGG - chr1_3:34343919-34343938 Msa0115460:intron 40.0%
TTCAGAGAGAAAAAAGGGGA+GGG + chr1_3:34344117-34344136 None:intergenic 40.0%
TTTCAGAGAGAAAAAAGGGG+AGG + chr1_3:34344118-34344137 None:intergenic 40.0%
! CTGATTCAATACAGGGTACT+AGG + chr1_3:34344353-34344372 None:intergenic 40.0%
AGTACCCTGTATTGAATCAG+TGG - chr1_3:34344353-34344372 Msa0115460:intron 40.0%
GTACCCTGTATTGAATCAGT+GGG - chr1_3:34344354-34344373 Msa0115460:intron 40.0%
!! TGTCCCACTGATTCAATACA+GGG + chr1_3:34344360-34344379 None:intergenic 40.0%
! AGCCAAAGCACTTTCTAAAC+GGG - chr1_3:34344532-34344551 Msa0115460:intron 40.0%
TGAAAAGAAGATGCAGGAGA+AGG - chr1_3:34344574-34344593 Msa0115460:intron 40.0%
TTTCAAAGTCGTGTGCAAGT+TGG - chr1_3:34344717-34344736 Msa0115460:intron 40.0%
! TGTTTTCCCAGGATACTTAC+AGG - chr1_3:34344750-34344769 Msa0115460:intron 40.0%
CCTATATCCTGTAAGTATCC+TGG + chr1_3:34344760-34344779 None:intergenic 40.0%
CCAGGATACTTACAGGATAT+AGG - chr1_3:34344757-34344776 Msa0115460:intron 40.0%
ATCAGAGGGAAGGTAATGAT+AGG - chr1_3:34344890-34344909 Msa0115460:intron 40.0%
TTTAAAAACCAGGAGGGACA+TGG + chr1_3:34344916-34344935 None:intergenic 40.0%
AACTCATCCAAAACCCATAC+GGG + chr1_3:34345112-34345131 None:intergenic 40.0%
CAACTCATCCAAAACCCATA+CGG + chr1_3:34345113-34345132 None:intergenic 40.0%
!! CTGCAGCTTTTGAAGAAGCA+TGG - chr1_3:34341416-34341435 Msa0115460:three_prime_UTR 45.0%
!!! GTGCTTTTGAAGTTCCTGGA+TGG - chr1_3:34341589-34341608 Msa0115460:three_prime_UTR 45.0%
!!! CTTTTGAAGTTCCTGGATGG+CGG - chr1_3:34341592-34341611 Msa0115460:three_prime_UTR 45.0%
!! GATGGCGGAAGTTACCTTTA+AGG - chr1_3:34341607-34341626 Msa0115460:exon 45.0%
ACAGGGTAGATTAGAACCTG+TGG - chr1_3:34341942-34341961 Msa0115460:intron 45.0%
CTGCTTATTGTCTCCTTTGG+AGG + chr1_3:34342049-34342068 None:intergenic 45.0%
CTCCAAAGGAGACAATAAGC+AGG - chr1_3:34342047-34342066 Msa0115460:intron 45.0%
AAATTTGCAATGCCTGCCGA+AGG - chr1_3:34342192-34342211 Msa0115460:intron 45.0%
CTCCCAAACATAAGCAAAGC+TGG + chr1_3:34342664-34342683 None:intergenic 45.0%
! CGACAATCTTTAGAAGCAGG+TGG - chr1_3:34343098-34343117 Msa0115460:intron 45.0%
CTGACATTGTATGCCCCTTT+AGG + chr1_3:34343215-34343234 None:intergenic 45.0%
GATGGTAGAATCCCGACTTA+TGG - chr1_3:34343699-34343718 Msa0115460:intron 45.0%
TGCCCAAAACACCATAAGTC+GGG + chr1_3:34343713-34343732 None:intergenic 45.0%
TCAGAGAGAAAAAAGGGGAG+GGG + chr1_3:34344116-34344135 None:intergenic 45.0%
!! CTGTCCCACTGATTCAATAC+AGG + chr1_3:34344361-34344380 None:intergenic 45.0%
!! CACCCGTTTAGAAAGTGCTT+TGG + chr1_3:34344537-34344556 None:intergenic 45.0%
! GCGCTTTCTTTAGAGAGTTC+TGG - chr1_3:34344606-34344625 Msa0115460:intron 45.0%
!! GGAAGTGTCTTTAGAGCTAG+TGG + chr1_3:34344996-34345015 None:intergenic 45.0%
TTCTTGAGAGTTCCCCGTAT+GGG - chr1_3:34345096-34345115 Msa0115460:intron 45.0%
ACTCATCCAAAACCCATACG+GGG + chr1_3:34345111-34345130 None:intergenic 45.0%
!!! CGTATGGGTTTTGGATGAGT+TGG - chr1_3:34345111-34345130 Msa0115460:intron 45.0%
!! AAATTCAATAAAATAAAATA+AGG + chr1_3:34342283-34342302 None:intergenic 5.0%
!!! TTATTTTATTTTATTGAATT+TGG - chr1_3:34342282-34342301 Msa0115460:intron 5.0%
!! AATTAAAAATATATTTAAAG+TGG - chr1_3:34343305-34343324 Msa0115460:intron 5.0%
!!! ATTAAAAATATATTTAAAGT+GGG - chr1_3:34343306-34343325 Msa0115460:intron 5.0%
CATGGTTGGAAAGAAGGGAC+TGG - chr1_3:34341434-34341453 Msa0115460:three_prime_UTR 50.0%
!! TGGAAAGAAGGGACTGGTCT+TGG - chr1_3:34341440-34341459 Msa0115460:three_prime_UTR 50.0%
! TGGTCTTGGAATCTCTGAGC+AGG - chr1_3:34341454-34341473 Msa0115460:three_prime_UTR 50.0%
! CTTCTGATCGAAGGCGTGTT+CGG + chr1_3:34341564-34341583 None:intergenic 50.0%
!!! GTCCGTGCTTTTGAAGTTCC+TGG - chr1_3:34341585-34341604 Msa0115460:three_prime_UTR 50.0%
AAAGGTAACTTCCGCCATCC+AGG + chr1_3:34341606-34341625 None:intergenic 50.0%
TTGGAGGAATCACCGTGATC+AGG + chr1_3:34342033-34342052 None:intergenic 50.0%
GATCACGGTGATTCCTCCAA+AGG - chr1_3:34342033-34342052 Msa0115460:intron 50.0%
CAGAGAGAAAAAAGGGGAGG+GGG + chr1_3:34344115-34344134 None:intergenic 50.0%
CAAGGGAAAAGCCCCTTTCA+CGG - chr1_3:34344401-34344420 Msa0115460:intron 50.0%
! GTGTGCAAGTTGGATCTCGA+AGG - chr1_3:34344727-34344746 Msa0115460:intron 50.0%
!!! GATCTCGAAGGTGTTTTCCC+AGG - chr1_3:34344739-34344758 Msa0115460:intron 50.0%
GTTCTTGAGAGTTCCCCGTA+TGG - chr1_3:34345095-34345114 Msa0115460:intron 50.0%
! AGAGTTCCCCGTATGGGTTT+TGG - chr1_3:34345102-34345121 Msa0115460:intron 50.0%
GCACGGACACTTCTGATCGA+AGG + chr1_3:34341573-34341592 None:intergenic 55.0%
! GAATTTGGTGAGGTGGGGGT+TGG - chr1_3:34342297-34342316 Msa0115460:intron 55.0%
GGGGTGACGTTGCACAATAG+TGG - chr1_3:34343326-34343345 Msa0115460:intron 55.0%
ATGATAGGCCATGTCCCTCC+TGG - chr1_3:34344905-34344924 Msa0115460:intron 55.0%
GCGTGTTCGGTCTAAGCGTC+CGG + chr1_3:34341551-34341570 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 34341015 34345247 34341015 ID=Msa0115460;Name=Msa0115460
chr1_3 mRNA 34341015 34345247 34341015 ID=Msa0115460-mRNA-1;Parent=Msa0115460;Name=Msa0115460-mRNA-1;_AED=0.02;_eAED=0.02;_QI=92|1|1|1|0.75|0.6|5|604|122
chr1_3 exon 34341015 34341750 34341015 ID=Msa0115460-mRNA-1:exon:10863;Parent=Msa0115460-mRNA-1
chr1_3 exon 34344194 34344316 34344194 ID=Msa0115460-mRNA-1:exon:10862;Parent=Msa0115460-mRNA-1
chr1_3 exon 34344787 34344840 34344787 ID=Msa0115460-mRNA-1:exon:10861;Parent=Msa0115460-mRNA-1
chr1_3 exon 34344951 34345035 34344951 ID=Msa0115460-mRNA-1:exon:10860;Parent=Msa0115460-mRNA-1
chr1_3 exon 34345181 34345247 34345181 ID=Msa0115460-mRNA-1:exon:10859;Parent=Msa0115460-mRNA-1
chr1_3 five_prime_UTR 34345181 34345247 34345181 ID=Msa0115460-mRNA-1:five_prime_utr;Parent=Msa0115460-mRNA-1
chr1_3 five_prime_UTR 34345011 34345035 34345011 ID=Msa0115460-mRNA-1:five_prime_utr;Parent=Msa0115460-mRNA-1
chr1_3 CDS 34344951 34345010 34344951 ID=Msa0115460-mRNA-1:cds;Parent=Msa0115460-mRNA-1
chr1_3 CDS 34344787 34344840 34344787 ID=Msa0115460-mRNA-1:cds;Parent=Msa0115460-mRNA-1
chr1_3 CDS 34344194 34344316 34344194 ID=Msa0115460-mRNA-1:cds;Parent=Msa0115460-mRNA-1
chr1_3 CDS 34341619 34341750 34341619 ID=Msa0115460-mRNA-1:cds;Parent=Msa0115460-mRNA-1
chr1_3 three_prime_UTR 34341015 34341618 34341015 ID=Msa0115460-mRNA-1:three_prime_utr;Parent=Msa0115460-mRNA-1
Gene Sequence

>Msa0115460

ATGGAAGAAACGTTGGGTTCAACAACAACTACAATCAACTCTTCCAATATAGGGTTTCAGCTTTTGAAGAAGCATGGTTGGAAAGAAGGGACTGGTCTTGGAATCTCTGAGCAGGGTAGATTAGAACCTGTGGAAACTCGTGTAAAGAATAATAAACGAGGTTTGGGAGCAGATATAGTGAAGAAGAAGGTTGTGAAACCTGATCACGGTGATTCCTCCAAAGGAGACAATAAGCAGGAACAATTGTCTCAAAAGAAAGCCAAAGCACTTTCTAAACGGGTGAGAAAGATGCAAGAGATTGAAAAGAAGATGCAGGAGAAGGAATTCGAGCGCGCTTTCTTTAGAGAGTTCTGGCCTGAAAATGTATGA
Protein sequence

>Msa0115460

MEETLGSTTTTINSSNIGFQLLKKHGWKEGTGLGISEQGRLEPVETRVKNNKRGLGADIVKKKVVKPDHGDSSKGDNKQEQLSQKKAKALSKRVRKMQEIEKKMQEKEFERAFFREFWPENV*