AlfalfaGEDB | Msa0182280

M. sativa cultivar zhongmu-4 / Msa0182280

KEGG: K12160
GO: -
PFAM: PF11976.11

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0182280	XP_013470130.1	95.000	220	7	1	1	220	1	216	7.43e-146	415

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0182280	G8A288	95.000	220	7	1	1	220	1	216	3.55e-146	415

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0182280	-0.846495	2.096220e-59	-8.615850e-47
Msa0004060	Msa0182280	0.830588	2.699427e-55	-8.615850e-47
Msa0006290	Msa0182280	0.818471	1.927097e-52	-8.615850e-47
Msa0006880	Msa0182280	0.837440	5.192028e-57	-8.615850e-47
Msa0010820	Msa0182280	0.826068	3.322584e-54	-8.615850e-47
Msa0015680	Msa0182280	0.825968	3.509707e-54	-8.615850e-47
Msa0026150	Msa0182280	0.816905	4.346512e-52	-8.615850e-47
Msa0026660	Msa0182280	0.800468	1.421309e-48	-8.615850e-47
Msa0027580	Msa0182280	0.854411	1.253348e-61	-8.615850e-47
Msa0029260	Msa0182280	0.814778	1.295334e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0182280	MtrunA17_Chr1g0209081	95.000	220	7	1	1	220	1	216	6.83e-150	415

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0182280	AT1G68185.1	47.085	223	107	6	1	220	1	215	1.05e-49	161
Msa0182280	AT1G68185.3	47.085	223	107	6	1	220	1	215	1.05e-49	161
Msa0182280	AT1G68185.2	47.085	223	107	6	1	220	47	261	7.82e-49	160

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Find 70 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTTACAGCCTTAGAG+AGG	0.611672	1_4:+70688833	None:intergenic
AAAATATGTACTGTACAATT+AGG	0.339197	1_4:+70689064	None:intergenic
AAACAAGGGTTCAAGTTCTT+CGG	0.392297	1_4:+70691570	None:intergenic
AAAGCAGATTCGGATGTTTA+TGG	0.264643	1_4:-70690445	Msa0182280:intron
AAAGGTTACAGCCTTAGAGA+GGG	0.685304	1_4:+70688834	None:intergenic
AACCAAATTTGTATAACATG+AGG	0.554290	1_4:-70688781	Msa0182280:three_prime_UTR
AACCCCTGCCAGCCTTGAAA+TGG	0.394451	1_4:-70690074	Msa0182280:CDS
AACGCGACTGTAATCAAACA+AGG	0.602179	1_4:+70691555	None:intergenic
AATAATTAATGATTATATTT+TGG	0.153894	1_4:+70688881	None:intergenic
AATCCAGACAGCGATAATGG+CGG	0.717060	1_4:-70691686	Msa0182280:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATTCTTCTTTCTCTTCTT+AGG	+	chr1_4:70689193-70689212	None:intergenic	30.0%
TTTGTTTACTTAACACTCCT+AGG	-	chr1_4:70689535-70689554	Msa0182280:intron	30.0%
AATTAAGCCTATTCTTGGTT+TGG	-	chr1_4:70689790-70689809	Msa0182280:intron	30.0%
TAAAGTTCCAAACCAAGAAT+AGG	+	chr1_4:70689800-70689819	None:intergenic	30.0%
TTTGTATAGATTGAAGAAGC+AGG	-	chr1_4:70689914-70689933	Msa0182280:intron	30.0%
ATCCTACAGAGAATTGAAAA+AGG	+	chr1_4:70690359-70690378	None:intergenic	30.0%
GGTAAATATCATTTGTAAGG+AGG	-	chr1_4:70690909-70690928	Msa0182280:intron	30.0%
AGAACAATGCAATAATGCAA+AGG	+	chr1_4:70691178-70691197	None:intergenic	30.0%
ATGATGTTGTGCATTATGTT+TGG	-	chr1_4:70691598-70691617	Msa0182280:intron	30.0%
TGATGTTGTGCATTATGTTT+GGG	-	chr1_4:70691599-70691618	Msa0182280:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	70688740	70691836	70688740	ID=Msa0182280;Name=Msa0182280
chr1_4	mRNA	70688740	70691836	70688740	ID=Msa0182280-mRNA-1;Parent=Msa0182280;Name=Msa0182280-mRNA-1;_AED=0.07;_eAED=0.07;_QI=144\|1\|1\|1\|0.83\|0.71\|7\|471\|220
chr1_4	exon	70688740	70689205	70688740	ID=Msa0182280-mRNA-1:exon:23671;Parent=Msa0182280-mRNA-1
chr1_4	exon	70690028	70690200	70690028	ID=Msa0182280-mRNA-1:exon:23670;Parent=Msa0182280-mRNA-1
chr1_4	exon	70690446	70690653	70690446	ID=Msa0182280-mRNA-1:exon:23669;Parent=Msa0182280-mRNA-1
chr1_4	exon	70691109	70691251	70691109	ID=Msa0182280-mRNA-1:exon:23668;Parent=Msa0182280-mRNA-1
chr1_4	exon	70691348	70691415	70691348	ID=Msa0182280-mRNA-1:exon:23667;Parent=Msa0182280-mRNA-1
chr1_4	exon	70691530	70691599	70691530	ID=Msa0182280-mRNA-1:exon:23666;Parent=Msa0182280-mRNA-1
chr1_4	exon	70691687	70691836	70691687	ID=Msa0182280-mRNA-1:exon:23665;Parent=Msa0182280-mRNA-1
chr1_4	five_prime_UTR	70691693	70691836	70691693	ID=Msa0182280-mRNA-1:five_prime_utr;Parent=Msa0182280-mRNA-1
chr1_4	CDS	70691687	70691692	70691687	ID=Msa0182280-mRNA-1:cds;Parent=Msa0182280-mRNA-1
chr1_4	CDS	70691530	70691599	70691530	ID=Msa0182280-mRNA-1:cds;Parent=Msa0182280-mRNA-1
chr1_4	CDS	70691348	70691415	70691348	ID=Msa0182280-mRNA-1:cds;Parent=Msa0182280-mRNA-1
chr1_4	CDS	70691109	70691251	70691109	ID=Msa0182280-mRNA-1:cds;Parent=Msa0182280-mRNA-1
chr1_4	CDS	70690446	70690653	70690446	ID=Msa0182280-mRNA-1:cds;Parent=Msa0182280-mRNA-1
chr1_4	CDS	70690033	70690200	70690033	ID=Msa0182280-mRNA-1:cds;Parent=Msa0182280-mRNA-1
chr1_4	three_prime_UTR	70690028	70690032	70690028	ID=Msa0182280-mRNA-1:three_prime_utr;Parent=Msa0182280-mRNA-1
chr1_4	three_prime_UTR	70688740	70689205	70688740	ID=Msa0182280-mRNA-1:three_prime_utr;Parent=Msa0182280-mRNA-1

Gene Sequence

>Msa0182280

ATGGCGGATGAAACCGAAGAACTTGAACCCTTGTTTGATTACAGTCGCGTTCAACCCAATATCGTGTGCCTCGATGATGATGAAGACGATGATGTTGTTGCTGTTCCTAAGAAGAGAAAGAAGAATTCTAAGTCTCAACATGTAGTTGAGAATGGGAAGAATACTAAGGTGGAAACAGTGAATGTGGTGGACCTCGAGGATGATGATTGGTTACCTTCGCCACCAAGGGTCACCGGTAAGGCCCATAAGAAGACTGATGAGGAGGATTCAACTTTGAAGAATTTGAGATTGAAGAAGCAGGAGCTTGCCGCTTTTGCTGAATCAGCAAAAAAATTGTTGAAAACTGTTGAGAATTCTGTAGAAATTGAAAATTCCAATTCATTGCAACCTTCCGTGGACGACGTAAGTGAAAAAGCGTCAAAACCTTCTGAACGAGCAAAGATTCTCATCTCTGTTCAAGACAAGGATGAAACAAAGCAGATTCGGATGTTTATGGATGACCAGTTTGAAAGGATTATCAAAACATATGCTGAGAAAATGAAGTGTGATCTGAAACAAATTGTGTTGTCATTTGATGGTGATAAAATAAGTTCATCTCAAACCCCTGCCAGCCTTGAAATGGAAGATAATGATATTATTGAAGTTCATGTGAAGTCAAGTTGA

Protein sequence

>Msa0182280

MADETEELEPLFDYSRVQPNIVCLDDDEDDDVVAVPKKRKKNSKSQHVVENGKNTKVETVNVVDLEDDDWLPSPPRVTGKAHKKTDEEDSTLKNLRLKKQELAAFAESAKKLLKTVENSVEIENSNSLQPSVDDVSEKASKPSERAKILISVQDKDETKQIRMFMDDQFERIIKTYAEKMKCDLKQIVLSFDGDKISSSQTPASLEMEDNDIIEVHVKSS*