AlfalfaGEDB | Msa1280530

M. sativa cultivar zhongmu-4 / Msa1280530

KEGG: K13459
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1280530	GAU40301.1	67.616	281	80	2	3	281	537	808	2.30e-121	376

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1280530	A0A2Z6N9B3	67.616	281	80	2	3	281	537	808	1.10e-121	376

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004180	Msa1280530	-0.808492	3.015538e-50	-8.615850e-47
Msa0025570	Msa1280530	0.804811	1.806016e-49	-8.615850e-47
Msa0027020	Msa1280530	-0.824105	9.667588e-54	-8.615850e-47
Msa0073110	Msa1280530	0.815534	8.798780e-52	-8.615850e-47
Msa0074860	Msa1280530	-0.801323	9.505847e-49	-8.615850e-47
Msa0075820	Msa1280530	-0.801828	7.488820e-49	-8.615850e-47
Msa0093360	Msa1280530	-0.807369	5.227930e-50	-8.615850e-47
Msa0107830	Msa1280530	0.811720	6.079659e-51	-8.615850e-47
Msa0117650	Msa1280530	0.811147	8.097584e-51	-8.615850e-47
Msa0139680	Msa1280530	0.803650	3.149731e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1280530	MtrunA17_Chr8g0348201	32.042	284	172	5	1	282	178	442	2.00e-37	141
Msa1280530	MtrunA17_Chr8g0348201	28.922	204	139	4	59	259	731	931	1.16e-19	89.4
Msa1280530	MtrunA17_Chr8g0348201	27.027	259	162	6	39	272	1221	1477	2.25e-19	88.6
Msa1280530	MtrunA17_Chr2g0313541	33.813	278	173	5	1	274	988	1258	2.40e-37	141
Msa1280530	MtrunA17_Chr2g0313541	27.749	191	122	5	85	271	1335	1513	1.33e-11	65.5
Msa1280530	MtrunA17_Chr2g0313591	33.898	295	175	7	1	290	980	1259	2.82e-37	140
Msa1280530	MtrunA17_Chr3g0081941	32.955	264	171	4	1	264	976	1233	5.60e-35	134
Msa1280530	MtrunA17_Chr2g0313331	34.926	272	159	7	1	265	673	933	4.35e-34	131
Msa1280530	MtrunA17_Chr2g0313461	34.066	273	161	7	1	265	979	1240	1.54e-33	130
Msa1280530	MtrunA17_Chr2g0314301	32.881	295	178	7	1	290	990	1269	1.62e-32	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 54 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTGATGGTGGTTAGTT+TGG	0.270799	8_3:+19157094	None:intergenic
AAATGGAGCAATGGTCGGAA+TGG	0.524129	8_3:-19157314	Msa1280530:CDS
AACATTCCCTCCACGTAACA+AGG	0.565384	8_3:+19157482	None:intergenic
AACTGGAGCATGTATGGAAA+TGG	0.416327	8_3:-19157331	Msa1280530:CDS
AACTTGATGGTGGTTAGTTT+GGG	0.337126	8_3:+19157095	None:intergenic
AAGCGCCTGGCTACAGAAAC+TGG	0.332009	8_3:+19157224	None:intergenic
AATGGAGCAATGGTCGGAAT+GGG	0.497571	8_3:-19157313	Msa1280530:CDS
ACTAAGTAGTATGAAGATTT+TGG	0.227799	8_3:-19157597	Msa1280530:CDS
ACTGGATACTTTGATCATTG+AGG	0.554897	8_3:-19157531	Msa1280530:CDS
AGAAGAGTGTAAAAGCTAAC+CGG	0.487818	8_3:+19156912	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCACACATTCACTTATGAAA+AGG	+	chr8_3:19156919-19156938	None:intergenic	30.0%
AGGATAACTATCTTTCTCTT+GGG	+	chr8_3:19156960-19156979	None:intergenic	30.0%
AGTATGGCTTCACTTTAATA+TGG	-	chr8_3:19157022-19157041	Msa1280530:CDS	30.0%
AATCCCATAAGAGAAATCAA+TGG	+	chr8_3:19157116-19157135	None:intergenic	30.0%
TTGATTTCTCTTATGGGATT+TGG	-	chr8_3:19157116-19157135	Msa1280530:CDS	30.0%
AGATAAGAAACAACTATGGA+GGG	+	chr8_3:19157149-19157168	None:intergenic	30.0%
AAGATAAGAAACAACTATGG+AGG	+	chr8_3:19157150-19157169	None:intergenic	30.0%
TAGGAAAGTAACAAACTTGA+AGG	-	chr8_3:19158077-19158096	Msa1280530:intron	30.0%
TCACACATTCACTTATGAAA+AGG	+	chr8_3:19156919-19156938	None:intergenic	30.0%
AGGATAACTATCTTTCTCTT+GGG	+	chr8_3:19156960-19156979	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	19156887	19158590	19156887	ID=Msa1280530;Name=Msa1280530
chr8_3	mRNA	19156887	19158590	19156887	ID=Msa1280530-mRNA-1;Parent=Msa1280530;Name=Msa1280530-mRNA-1;_AED=0.93;_eAED=0.93;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|297
chr8_3	exon	19158495	19158590	19158495	ID=Msa1280530-mRNA-1:exon:7155;Parent=Msa1280530-mRNA-1
chr8_3	exon	19156887	19157684	19156887	ID=Msa1280530-mRNA-1:exon:7154;Parent=Msa1280530-mRNA-1
chr8_3	CDS	19158495	19158590	19158495	ID=Msa1280530-mRNA-1:cds;Parent=Msa1280530-mRNA-1
chr8_3	CDS	19156887	19157684	19156887	ID=Msa1280530-mRNA-1:cds;Parent=Msa1280530-mRNA-1

Gene Sequence

>Msa1280530

ATGGCTGAGAGTTTACTGAATCTGCAAAGCCTTTTCATAAGTGAATGTGTGAAGATGAGACACATCTTTTCCCAAGAGAAAGATAGTTATCCTGAGTTAATTAACTTATGGGTATTTGAACCAAAACAGGGTAGCATCTTTCCAAATTTGAAGACTATCAAACTAAGTAGTATGAAGATTTTGGTGGAGATATGGAACTCTGAACTCTCTTCAAATTCCTTTGGCAAACTGGATACTTTGATCATTGAGGAGTGTGACAAATTTATTGTCGTTTTTCCTTGTTACGTGGAGGGAATGTTTCAGCATCTATGCAACTTGAGGGTTACAAATTGTAAATCTCTCAAAGCAGTATTTGAATTAGATGATAAAAAACGACATAGTAGGAAAGTAACAAACTTGAAGGATATTTATTTAGAAACACTCCCAAAACTGGAGCATGTATGGAAATGGAGCAATGGTCGGAATGGGATTCTTGAATTAAAAAGTGTGCAGAAGTTGTGGGTTCGTGACTGTCTAAGCTTAGAAAATATATTTCCAGTTTCTGTAGCCAGGCGCTTTCACAGTCTTGCGTACCTTGTAATATGGGATTGCTATCAATTGAGGGAAATTGTTGCCAAGAGAGAAGGTACTGGGAGCAACATTCTTGATTCATCCTTTGATTTTCCCAAACTAACCACCATCAAGTTTTCGGAACTACCAATGCTTACGTGCTTCTGTCCAGGAGATTATAAATTCAATTGTCCAATACTGAATGACCTGTCTATTGAGCTTTGTGACAGACTGGAACCGTTCAGAAAAGAAACCACAGATGCACAAACAAATTCAACCCTCTTTTCTGAAGAGGTACCGGTTAGCTTTTACACTCTTCTAAGAAATTTTTCTACTTCATTGTAA

Protein sequence

>Msa1280530

MAESLLNLQSLFISECVKMRHIFSQEKDSYPELINLWVFEPKQGSIFPNLKTIKLSSMKILVEIWNSELSSNSFGKLDTLIIEECDKFIVVFPCYVEGMFQHLCNLRVTNCKSLKAVFELDDKKRHSRKVTNLKDIYLETLPKLEHVWKWSNGRNGILELKSVQKLWVRDCLSLENIFPVSVARRFHSLAYLVIWDCYQLREIVAKREGTGSNILDSSFDFPKLTTIKFSELPMLTCFCPGDYKFNCPILNDLSIELCDRLEPFRKETTDAQTNSTLFSEEVPVSFYTLLRNFSTSL*