AlfalfaGEDB | Msa1333220

M. sativa cultivar zhongmu-4 / Msa1333220

KEGG: K00951
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008728 GO:0009507 GO:0009536 GO:0016740 GO:0016772 GO:0016778 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF13328.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1333220	XP_003609875.1	96.207	580	18	2	33	608	1	580	0.0	1132

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1333220	sp\|Q84R11\|CRSH_ARATH	69.275	524	154	3	90	606	57	580	0.0	712

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1333220	G7JR05	96.207	580	18	2	33	608	1	580	0.0	1132

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa1333220	0.835718	1.425690e-56	-8.615850e-47
Msa0000800	Msa1333220	-0.809160	2.171023e-50	-8.615850e-47
Msa0002110	Msa1333220	0.883564	4.031206e-71	-8.615850e-47
Msa0002690	Msa1333220	0.859694	3.464321e-63	-8.615850e-47
Msa0002700	Msa1333220	0.864921	8.586290e-65	-8.615850e-47
Msa0003840	Msa1333220	-0.846515	2.069624e-59	-8.615850e-47
Msa0004180	Msa1333220	0.840415	8.818073e-58	-8.615850e-47
Msa0005500	Msa1333220	0.876676	1.154950e-68	-8.615850e-47
Msa0007570	Msa1333220	0.816758	4.688810e-52	-8.615850e-47
Msa0007810	Msa1333220	0.911096	8.946141e-83	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1333220	MtrunA17_Chr4g0069291	96.207	580	18	2	33	608	1	580	0.0	1132
Msa1333220	MtrunA17_Chr7g0269591	29.752	363	218	8	148	484	175	526	1.04e-39	155
Msa1333220	MtrunA17_Chr8g0344131	29.261	352	212	7	140	484	242	563	1.27e-33	136
Msa1333220	MtrunA17_Chr5g0395711	36.719	128	74	3	335	461	443	564	2.54e-14	76.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1333220	AT3G17470.2	69.275	524	154	3	90	606	72	595	0.0	712
Msa1333220	AT3G17470.1	69.275	524	154	3	90	606	57	580	0.0	712
Msa1333220	AT3G17470.3	68.668	383	118	1	90	470	57	439	0.0	543
Msa1333220	AT1G54130.1	32.530	332	191	7	140	463	234	540	5.10e-38	150
Msa1333220	AT3G14050.1	32.530	332	190	9	141	463	230	536	7.72e-37	146
Msa1333220	AT4G02260.3	29.969	327	199	7	148	455	177	492	1.11e-36	146
Msa1333220	AT4G02260.2	29.787	329	201	7	148	457	177	494	1.88e-36	146
Msa1333220	AT4G02260.4	29.787	329	201	7	148	457	177	494	1.95e-36	146
Msa1333220	AT4G02260.1	29.697	330	201	8	148	457	177	495	6.68e-35	141

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Find 165 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATTCCTCTTTACAAA+CGG	0.460364	8_4:-53257678	None:intergenic
AAAATCTGATCCCATACTTG+CGG	0.616260	8_4:+53256259	Msa1333220:CDS
AAGAAAACACTAACCTCCTC+TGG	0.456445	8_4:-53256689	None:intergenic
AAGAAGACCAGAGGAACTTG+TGG	0.542935	8_4:-53254664	None:intergenic
AAGATGGTGATAGAGCTTGT+CGG	0.658286	8_4:+53254603	Msa1333220:CDS
AAGCATTGAGGAACTTACAG+AGG	0.688318	8_4:+53257016	Msa1333220:CDS
AATGCATTATCTCCAGACTT+AGG	0.376086	8_4:-53256410	None:intergenic
ACAGAGGTGATAGAGGAGCT+TGG	0.612835	8_4:+53257032	Msa1333220:CDS
ACAGATAAGAACTACTGAAA+TGG	0.407491	8_4:+53256607	Msa1333220:CDS
ACCACCGGAGACCGGTGACG+CGG	0.582141	8_4:-53254539	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAGTTTCATCCACAATAT+TGG	+	chr8_4:53254328-53254347	Msa1333220:CDS	30.0%
TTGAGACTTGAACCTAAAAA+AGG	-	chr8_4:53254993-53255012	None:intergenic	30.0%
CATTGAGTAGTTAATGTATG+TGG	-	chr8_4:53255080-53255099	None:intergenic	30.0%
ATAATTTGGTTATGTGTCTC+TGG	-	chr8_4:53255503-53255522	None:intergenic	30.0%
TTGCAGTTAACAAAACACAT+AGG	+	chr8_4:53255533-53255552	Msa1333220:intron	30.0%
AACAGCTACTATACTCTTAA+TGG	-	chr8_4:53255572-53255591	None:intergenic	30.0%
CAATGAAATTATGACACTCA+AGG	-	chr8_4:53255709-53255728	None:intergenic	30.0%
ATAGTAAATGTGATTGCAGA+TGG	+	chr8_4:53255746-53255765	Msa1333220:intron	30.0%
CATATCTTTATGTGGATACA+TGG	+	chr8_4:53256168-53256187	Msa1333220:CDS	30.0%
ATCAAATCATCCAATCTATG+TGG	+	chr8_4:53256465-53256484	Msa1333220:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	53254246	53257747	53254246	ID=Msa1333220;Name=Msa1333220
chr8_4	mRNA	53254246	53257747	53254246	ID=Msa1333220-mRNA-1;Parent=Msa1333220;Name=Msa1333220-mRNA-1;_AED=0.15;_eAED=0.15;_QI=81\|1\|1\|1\|1\|1\|4\|234\|608
chr8_4	exon	53254246	53254800	53254246	ID=Msa1333220-mRNA-1:exon:16255;Parent=Msa1333220-mRNA-1
chr8_4	exon	53255765	53256697	53255765	ID=Msa1333220-mRNA-1:exon:16256;Parent=Msa1333220-mRNA-1
chr8_4	exon	53256867	53257151	53256867	ID=Msa1333220-mRNA-1:exon:16257;Parent=Msa1333220-mRNA-1
chr8_4	exon	53257379	53257747	53257379	ID=Msa1333220-mRNA-1:exon:16258;Parent=Msa1333220-mRNA-1
chr8_4	five_prime_UTR	53254246	53254326	53254246	ID=Msa1333220-mRNA-1:five_prime_utr;Parent=Msa1333220-mRNA-1
chr8_4	CDS	53254327	53254800	53254327	ID=Msa1333220-mRNA-1:cds;Parent=Msa1333220-mRNA-1
chr8_4	CDS	53255765	53256697	53255765	ID=Msa1333220-mRNA-1:cds;Parent=Msa1333220-mRNA-1
chr8_4	CDS	53256867	53257151	53256867	ID=Msa1333220-mRNA-1:cds;Parent=Msa1333220-mRNA-1
chr8_4	CDS	53257379	53257513	53257379	ID=Msa1333220-mRNA-1:cds;Parent=Msa1333220-mRNA-1
chr8_4	three_prime_UTR	53257514	53257747	53257514	ID=Msa1333220-mRNA-1:three_prime_utr;Parent=Msa1333220-mRNA-1

Gene Sequence

>Msa1333220

ATGAAGTTTCATCCACAATATTGGTGTTGTGTTTCTTTGTCACAACCAACAACATTACATTACATTCATAGATGTCAACTACGCATTGAACCAAAAATGGAACTCTTACACTTCCACGCTTCCGATTACCCCACGCGCCACCATTCTCTTCTCAAACAAACCACTACCACTCACCACCGCTTACTCTCACTCTTCTTCCCCACTCGACCCACCCGCGTCACCGGTCTCCGGTGGTCCACTCCGAGAGCGTGTGCTGTAGAAGTTCCCGGTGGAGGAAAGATGGTGATAGAGCTTGTCGGTGCTTTCAATGATTTGACTGAGAGGATGAAGGTGTTATCCACAAGTTCCTCTGGTCTTCTTTTCAAATCATTGAAACTCTCCATTCCTGTTCTTCAAAGCTCTCCTCTTACTCCTGATGGTCGCTCTCCTCTTTCCAAAGCTTTATCCATTGCCATGCTTCTCGCTGATCTTCAGATGGATGCAGAAGTTATATCTGCTGGAATATTGAGAGAGGTGTTGGAAGTAGGTGAGTTAAATCTACATGAAGTTCGAAGTCAGATAGGTTCTGCTACTGCTCATTTGGTGCACGAGAGTTTGCGTGTTAAGAACTTTGCATCTAGAGTAGACATTCTGGATGAAGAGAATGCTGCTGCTTTGAGAAAGTTCTGTCTCACTTACTATGACATTAGAGCTTTGATTTTGGACTTGGCTTTGAAGCTTGACATGATGAGGCATCTTGGTCACCTTCCAAGGTATCAGCAGCAGATCATTTCTCTGCAAGTTATGAAAATATATGCTCCTCTTGCTCATGCTGTGGGAATTAACTACATATCACTTGAATTGGAAGATCTCTCGTTTCAGTATTTGTTTCCTTATTCATATCTTTATGTGGATACATGGTTGAGAAGTCAGGAGACAGGTGGTATATCTCTTATCGATGTATACAAAGATGAATTGCTTCAGTCACTAAAATCTGATCCCATACTTGCGGAATTAGTGGATGATATCTCAGTTAAAGGTCGTTATAAAAGTCGTTACAGCACAATGAAGAAGCTCTTGAAGGATGGTCGGAGACCAGAAGATGTAAATGATGTGCTTGGACTGCGAGTGGTGTTGAACCCTAAGTCTGGAGATAATGCATTGGAAGCTGGAGAGAGAGCGTGCTACAGGGCTCATCAAATCATCCAATCTATGTGGAAAGAAATCCCTTCTCGGACTAAAGATTATATTGCCAGGCCAAAAGGGAATGGATACAAAAGTTTGCATATGGCAGTGGATGTAAGTGAAATTGGGAGGACTAGACCCTTGATGGAAATACAGATAAGAACTACTGAAATGGATAGGTTAGCTGTTGGTGGAATGGCATCTCATTCATTATACAAGGCTGGTCTTACTAATCCAGAGGAGGCAAAGCGTTTAAAGACCATCATGCTGGCTGCAGCTGAACTGGCTGCTTTGCGCCTTAAAGATTTTCCAAGTGCAAATCATAAAGGGATTGAATTTGATCAGAGAGATAGAGTATTTCGCCTTCTCGACAAGAACGGAGATGGGAAGATAAGCATTGAGGAACTTACAGAGGTGATAGAGGAGCTTGGTGCACCAGGAGAAGATGCACATGACATGATGCTGCTTCTTGACTCGAACAGTGATGGCTCCCTCAGCTCTGATGAATTTCAGATGTTCCAAAAACAGGTCGAGATGGTTCGTAATTTAGAGGACAGAGATGATGAATACAAGAAAATATTGGATGAGAAACTTCATATGGCAGATGATAGTGGTTTAATTCAGGTATATAATAAGGAGTTTGGCAACAGGCTTGTGAGCTAG

Protein sequence

>Msa1333220

MKFHPQYWCCVSLSQPTTLHYIHRCQLRIEPKMELLHFHASDYPTRHHSLLKQTTTTHHRLLSLFFPTRPTRVTGLRWSTPRACAVEVPGGGKMVIELVGAFNDLTERMKVLSTSSSGLLFKSLKLSIPVLQSSPLTPDGRSPLSKALSIAMLLADLQMDAEVISAGILREVLEVGELNLHEVRSQIGSATAHLVHESLRVKNFASRVDILDEENAAALRKFCLTYYDIRALILDLALKLDMMRHLGHLPRYQQQIISLQVMKIYAPLAHAVGINYISLELEDLSFQYLFPYSYLYVDTWLRSQETGGISLIDVYKDELLQSLKSDPILAELVDDISVKGRYKSRYSTMKKLLKDGRRPEDVNDVLGLRVVLNPKSGDNALEAGERACYRAHQIIQSMWKEIPSRTKDYIARPKGNGYKSLHMAVDVSEIGRTRPLMEIQIRTTEMDRLAVGGMASHSLYKAGLTNPEEAKRLKTIMLAAAELAALRLKDFPSANHKGIEFDQRDRVFRLLDKNGDGKISIEELTEVIEELGAPGEDAHDMMLLLDSNSDGSLSSDEFQMFQKQVEMVRNLEDRDDEYKKILDEKLHMADDSGLIQVYNKEFGNRLVS*