Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36056.t1 | XP_003628540.1 | 91.2 | 147 | 12 | 1 | 1 | 146 | 1 | 147 | 1.50E-54 | 222.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36056.t1 | Q94KR7 | 84.1 | 82 | 13 | 0 | 59 | 140 | 54 | 135 | 5.2e-35 | 148.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36056.t1 | G7LDQ8 | 91.2 | 147 | 12 | 1 | 1 | 146 | 1 | 147 | 1.1e-54 | 222.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene36056 | 0.83187 | 1.31E-55 | -1.69E-46 |
| MS.gene049171 | MS.gene36056 | 0.937996 | 1.39E-98 | -1.69E-46 |
| MS.gene049173 | MS.gene36056 | 0.86069 | 1.73E-63 | -1.69E-46 |
| MS.gene049217 | MS.gene36056 | 0.863836 | 1.87E-64 | -1.69E-46 |
| MS.gene049222 | MS.gene36056 | 0.889457 | 2.38E-73 | -1.69E-46 |
| MS.gene049232 | MS.gene36056 | 0.817637 | 2.97E-52 | -1.69E-46 |
| MS.gene049281 | MS.gene36056 | -0.807595 | 4.68E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene36056 | 0.912368 | 2.11E-83 | -1.69E-46 |
| MS.gene049320 | MS.gene36056 | 0.902756 | 7.00E-79 | -1.69E-46 |
| MS.gene04938 | MS.gene36056 | 0.802173 | 6.36E-49 | -1.69E-46 |
| MS.gene04939 | MS.gene36056 | 0.856451 | 3.19E-62 | -1.69E-46 |
| MS.gene049609 | MS.gene36056 | 0.846334 | 2.32E-59 | -1.69E-46 |
| MS.gene049627 | MS.gene36056 | 0.814132 | 1.80E-51 | -1.69E-46 |
| MS.gene049633 | MS.gene36056 | 0.883926 | 2.97E-71 | -1.69E-46 |
| MS.gene049805 | MS.gene36056 | 0.83429 | 3.26E-56 | -1.69E-46 |
| MS.gene05010 | MS.gene36056 | 0.843892 | 1.06E-58 | -1.69E-46 |
| MS.gene050163 | MS.gene36056 | 0.916332 | 2.02E-85 | -1.69E-46 |
| MS.gene050165 | MS.gene36056 | 0.912314 | 2.24E-83 | -1.69E-46 |
| MS.gene050170 | MS.gene36056 | 0.846613 | 1.95E-59 | -1.69E-46 |
| MS.gene050323 | MS.gene36056 | 0.850063 | 2.16E-60 | -1.69E-46 |
| MS.gene050333 | MS.gene36056 | 0.880286 | 6.22E-70 | -1.69E-46 |
| MS.gene050338 | MS.gene36056 | -0.814706 | 1.34E-51 | -1.69E-46 |
| MS.gene050444 | MS.gene36056 | 0.837786 | 4.23E-57 | -1.69E-46 |
| MS.gene050496 | MS.gene36056 | 0.826566 | 2.53E-54 | -1.69E-46 |
| MS.gene050504 | MS.gene36056 | 0.912418 | 1.99E-83 | -1.69E-46 |
| MS.gene050518 | MS.gene36056 | 0.915309 | 6.86E-85 | -1.69E-46 |
| MS.gene050538 | MS.gene36056 | 0.801933 | 7.13E-49 | -1.69E-46 |
| MS.gene050577 | MS.gene36056 | 0.930657 | 1.19E-93 | -1.69E-46 |
| MS.gene050612 | MS.gene36056 | 0.916042 | 2.86E-85 | -1.69E-46 |
| MS.gene050675 | MS.gene36056 | 0.87417 | 8.32E-68 | -1.69E-46 |
| MS.gene050699 | MS.gene36056 | 0.916081 | 2.73E-85 | -1.69E-46 |
| MS.gene050747 | MS.gene36056 | 0.84953 | 3.05E-60 | -1.69E-46 |
| MS.gene050772 | MS.gene36056 | 0.863618 | 2.19E-64 | -1.69E-46 |
| MS.gene05080 | MS.gene36056 | 0.860908 | 1.49E-63 | -1.69E-46 |
| MS.gene05081 | MS.gene36056 | 0.804128 | 2.51E-49 | -1.69E-46 |
| MS.gene050832 | MS.gene36056 | -0.803097 | 4.10E-49 | -1.69E-46 |
| MS.gene050833 | MS.gene36056 | -0.801691 | 7.99E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene36056 | 0.849082 | 4.06E-60 | -1.69E-46 |
| MS.gene050871 | MS.gene36056 | 0.844485 | 7.34E-59 | -1.69E-46 |
| MS.gene050876 | MS.gene36056 | 0.848929 | 4.48E-60 | -1.69E-46 |
| MS.gene050904 | MS.gene36056 | 0.873824 | 1.09E-67 | -1.69E-46 |
| MS.gene050950 | MS.gene36056 | 0.843302 | 1.52E-58 | -1.69E-46 |
| MS.gene050986 | MS.gene36056 | 0.885911 | 5.40E-72 | -1.69E-46 |
| MS.gene05100 | MS.gene36056 | 0.897701 | 1.09E-76 | -1.69E-46 |
| MS.gene051138 | MS.gene36056 | -0.804607 | 1.99E-49 | -1.69E-46 |
| MS.gene051268 | MS.gene36056 | 0.810107 | 1.36E-50 | -1.69E-46 |
| MS.gene051317 | MS.gene36056 | 0.883898 | 3.04E-71 | -1.69E-46 |
| MS.gene051336 | MS.gene36056 | 0.816068 | 6.69E-52 | -1.69E-46 |
| MS.gene051551 | MS.gene36056 | 0.867204 | 1.63E-65 | -1.69E-46 |
| MS.gene051651 | MS.gene36056 | -0.821091 | 4.85E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene36056 | 0.850565 | 1.56E-60 | -1.69E-46 |
| MS.gene051810 | MS.gene36056 | 0.860154 | 2.52E-63 | -1.69E-46 |
| MS.gene051838 | MS.gene36056 | 0.807953 | 3.93E-50 | -1.69E-46 |
| MS.gene051858 | MS.gene36056 | 0.816647 | 4.96E-52 | -1.69E-46 |
| MS.gene051949 | MS.gene36056 | 0.83553 | 1.59E-56 | -1.69E-46 |
| MS.gene052129 | MS.gene36056 | -0.804134 | 2.50E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene36056 | 0.869572 | 2.80E-66 | -1.69E-46 |
| MS.gene052271 | MS.gene36056 | 0.853345 | 2.54E-61 | -1.69E-46 |
| MS.gene052379 | MS.gene36056 | 0.803471 | 3.43E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene36056 | 0.878658 | 2.35E-69 | -1.69E-46 |
| MS.gene052486 | MS.gene36056 | 0.883177 | 5.59E-71 | -1.69E-46 |
| MS.gene052503 | MS.gene36056 | 0.855524 | 5.96E-62 | -1.69E-46 |
| MS.gene052506 | MS.gene36056 | 0.83599 | 1.22E-56 | -1.69E-46 |
| MS.gene052615 | MS.gene36056 | 0.854982 | 8.56E-62 | -1.69E-46 |
| MS.gene052822 | MS.gene36056 | 0.84572 | 3.41E-59 | -1.69E-46 |
| MS.gene052823 | MS.gene36056 | 0.85246 | 4.55E-61 | -1.69E-46 |
| MS.gene052825 | MS.gene36056 | 0.845077 | 5.09E-59 | -1.69E-46 |
| MS.gene052870 | MS.gene36056 | 0.803331 | 3.67E-49 | -1.69E-46 |
| MS.gene052872 | MS.gene36056 | 0.820703 | 5.96E-53 | -1.69E-46 |
| MS.gene052916 | MS.gene36056 | 0.827328 | 1.66E-54 | -1.69E-46 |
| MS.gene052966 | MS.gene36056 | 0.821333 | 4.27E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene36056 | 0.806491 | 8.02E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene36056 | 0.864799 | 9.38E-65 | -1.69E-46 |
| MS.gene053207 | MS.gene36056 | 0.81151 | 6.75E-51 | -1.69E-46 |
| MS.gene053213 | MS.gene36056 | 0.868111 | 8.32E-66 | -1.69E-46 |
| MS.gene053284 | MS.gene36056 | 0.828005 | 1.14E-54 | -1.69E-46 |
| MS.gene053285 | MS.gene36056 | 0.816555 | 5.21E-52 | -1.69E-46 |
| MS.gene053286 | MS.gene36056 | 0.862912 | 3.62E-64 | -1.69E-46 |
| MS.gene053322 | MS.gene36056 | 0.812993 | 3.20E-51 | -1.69E-46 |
| MS.gene053436 | MS.gene36056 | 0.835142 | 1.99E-56 | -1.69E-46 |
| MS.gene053479 | MS.gene36056 | 0.812043 | 5.17E-51 | -1.69E-46 |
| MS.gene053480 | MS.gene36056 | 0.841076 | 5.92E-58 | -1.69E-46 |
| MS.gene053574 | MS.gene36056 | 0.845675 | 3.50E-59 | -1.69E-46 |
| MS.gene053626 | MS.gene36056 | 0.813832 | 2.10E-51 | -1.69E-46 |
| MS.gene053632 | MS.gene36056 | 0.879891 | 8.61E-70 | -1.69E-46 |
| MS.gene053824 | MS.gene36056 | 0.847987 | 8.17E-60 | -1.69E-46 |
| MS.gene053916 | MS.gene36056 | 0.833644 | 4.74E-56 | -1.69E-46 |
| MS.gene054408 | MS.gene36056 | 0.832907 | 7.23E-56 | -1.69E-46 |
| MS.gene054595 | MS.gene36056 | 0.857116 | 2.03E-62 | -1.69E-46 |
| MS.gene054600 | MS.gene36056 | 0.860739 | 1.67E-63 | -1.69E-46 |
| MS.gene054642 | MS.gene36056 | 0.895201 | 1.20E-75 | -1.69E-46 |
| MS.gene05470 | MS.gene36056 | 0.914268 | 2.34E-84 | -1.69E-46 |
| MS.gene054884 | MS.gene36056 | -0.802942 | 4.42E-49 | -1.69E-46 |
| MS.gene054954 | MS.gene36056 | 0.907122 | 7.13E-81 | -1.69E-46 |
| MS.gene054959 | MS.gene36056 | -0.802255 | 6.12E-49 | -1.69E-46 |
| MS.gene055154 | MS.gene36056 | -0.812883 | 3.39E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene36056 | 0.858542 | 7.67E-63 | -1.69E-46 |
| MS.gene055251 | MS.gene36056 | -0.815217 | 1.04E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene36056 | 0.901378 | 2.85E-78 | -1.69E-46 |
| MS.gene055397 | MS.gene36056 | 0.817775 | 2.77E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene36056 | 0.902622 | 8.03E-79 | -1.69E-46 |
| MS.gene055577 | MS.gene36056 | 0.872422 | 3.22E-67 | -1.69E-46 |
| MS.gene055647 | MS.gene36056 | 0.810445 | 1.15E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene36056 | 0.816855 | 4.46E-52 | -1.69E-46 |
| MS.gene055729 | MS.gene36056 | -0.801587 | 8.39E-49 | -1.69E-46 |
| MS.gene055730 | MS.gene36056 | -0.801058 | 1.08E-48 | -1.69E-46 |
| MS.gene055748 | MS.gene36056 | 0.908706 | 1.28E-81 | -1.69E-46 |
| MS.gene055750 | MS.gene36056 | 0.894199 | 3.09E-75 | -1.69E-46 |
| MS.gene055751 | MS.gene36056 | 0.904324 | 1.38E-79 | -1.69E-46 |
| MS.gene055776 | MS.gene36056 | 0.852924 | 3.35E-61 | -1.69E-46 |
| MS.gene055849 | MS.gene36056 | 0.839626 | 1.42E-57 | -1.69E-46 |
| MS.gene05589 | MS.gene36056 | 0.879227 | 1.48E-69 | -1.69E-46 |
| MS.gene055941 | MS.gene36056 | 0.846027 | 2.81E-59 | -1.69E-46 |
| MS.gene055956 | MS.gene36056 | -0.814512 | 1.48E-51 | -1.69E-46 |
| MS.gene056083 | MS.gene36056 | 0.8192 | 1.32E-52 | -1.69E-46 |
| MS.gene056374 | MS.gene36056 | 0.862193 | 6.03E-64 | -1.69E-46 |
| MS.gene056409 | MS.gene36056 | 0.817448 | 3.28E-52 | -1.69E-46 |
| MS.gene056450 | MS.gene36056 | 0.81206 | 5.13E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene36056 | 0.878022 | 3.93E-69 | -1.69E-46 |
| MS.gene056510 | MS.gene36056 | 0.882035 | 1.46E-70 | -1.69E-46 |
| MS.gene056549 | MS.gene36056 | 0.823986 | 1.03E-53 | -1.69E-46 |
| MS.gene056550 | MS.gene36056 | -0.805826 | 1.11E-49 | -1.69E-46 |
| MS.gene056610 | MS.gene36056 | 0.84734 | 1.23E-59 | -1.69E-46 |
| MS.gene056657 | MS.gene36056 | 0.895865 | 6.38E-76 | -1.69E-46 |
| MS.gene056692 | MS.gene36056 | 0.893563 | 5.59E-75 | -1.69E-46 |
| MS.gene056856 | MS.gene36056 | -0.829195 | 5.90E-55 | -1.69E-46 |
| MS.gene056898 | MS.gene36056 | 0.816796 | 4.60E-52 | -1.69E-46 |
| MS.gene056946 | MS.gene36056 | 0.802512 | 5.42E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene36056 | 0.8098 | 1.58E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene36056 | 0.840752 | 7.20E-58 | -1.69E-46 |
| MS.gene057332 | MS.gene36056 | 0.812092 | 5.04E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene36056 | 0.870956 | 9.86E-67 | -1.69E-46 |
| MS.gene057658 | MS.gene36056 | 0.863406 | 2.55E-64 | -1.69E-46 |
| MS.gene057831 | MS.gene36056 | 0.904135 | 1.68E-79 | -1.69E-46 |
| MS.gene05787 | MS.gene36056 | 0.808453 | 3.07E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene36056 | 0.820254 | 7.56E-53 | -1.69E-46 |
| MS.gene058334 | MS.gene36056 | -0.873035 | 2.01E-67 | -1.69E-46 |
| MS.gene058338 | MS.gene36056 | 0.847669 | 9.99E-60 | -1.69E-46 |
| MS.gene05837 | MS.gene36056 | 0.843508 | 1.34E-58 | -1.69E-46 |
| MS.gene058419 | MS.gene36056 | 0.926519 | 4.19E-91 | -1.69E-46 |
| MS.gene058544 | MS.gene36056 | 0.907113 | 7.20E-81 | -1.69E-46 |
| MS.gene058719 | MS.gene36056 | 0.817398 | 3.37E-52 | -1.69E-46 |
| MS.gene058723 | MS.gene36056 | 0.844109 | 9.26E-59 | -1.69E-46 |
| MS.gene058773 | MS.gene36056 | 0.861242 | 1.18E-63 | -1.69E-46 |
| MS.gene058838 | MS.gene36056 | 0.879675 | 1.03E-69 | -1.69E-46 |
| MS.gene05883 | MS.gene36056 | 0.818223 | 2.19E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene36056 | 0.907689 | 3.86E-81 | -1.69E-46 |
| MS.gene059023 | MS.gene36056 | 0.900812 | 5.03E-78 | -1.69E-46 |
| MS.gene059029 | MS.gene36056 | 0.813774 | 2.16E-51 | -1.69E-46 |
| MS.gene059079 | MS.gene36056 | 0.822558 | 2.22E-53 | -1.69E-46 |
| MS.gene05912 | MS.gene36056 | 0.834904 | 2.29E-56 | -1.69E-46 |
| MS.gene05914 | MS.gene36056 | 0.829976 | 3.81E-55 | -1.69E-46 |
| MS.gene05934 | MS.gene36056 | 0.835082 | 2.06E-56 | -1.69E-46 |
| MS.gene059667 | MS.gene36056 | 0.81193 | 5.47E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene36056 | 0.922312 | 1.16E-88 | -1.69E-46 |
| MS.gene059718 | MS.gene36056 | 0.877218 | 7.49E-69 | -1.69E-46 |
| MS.gene059756 | MS.gene36056 | 0.892256 | 1.88E-74 | -1.69E-46 |
| MS.gene059801 | MS.gene36056 | 0.825278 | 5.11E-54 | -1.69E-46 |
| MS.gene059837 | MS.gene36056 | 0.828849 | 7.15E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene36056 | 0.880618 | 4.73E-70 | -1.69E-46 |
| MS.gene059902 | MS.gene36056 | 0.814233 | 1.71E-51 | -1.69E-46 |
| MS.gene059953 | MS.gene36056 | -0.812397 | 4.33E-51 | -1.69E-46 |
| MS.gene059960 | MS.gene36056 | 0.803904 | 2.79E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene36056 | 0.843792 | 1.13E-58 | -1.69E-46 |
| MS.gene060337 | MS.gene36056 | -0.806151 | 9.46E-50 | -1.69E-46 |
| MS.gene060526 | MS.gene36056 | 0.806728 | 7.15E-50 | -1.69E-46 |
| MS.gene060703 | MS.gene36056 | 0.869792 | 2.37E-66 | -1.69E-46 |
| MS.gene060728 | MS.gene36056 | 0.813588 | 2.37E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene36056 | 0.891615 | 3.38E-74 | -1.69E-46 |
| MS.gene060778 | MS.gene36056 | 0.826277 | 2.96E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene36056 | 0.904451 | 1.21E-79 | -1.69E-46 |
| MS.gene060823 | MS.gene36056 | -0.814793 | 1.29E-51 | -1.69E-46 |
| MS.gene06084 | MS.gene36056 | -0.808764 | 2.64E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene36056 | 0.857951 | 1.15E-62 | -1.69E-46 |
| MS.gene060982 | MS.gene36056 | 0.885394 | 8.44E-72 | -1.69E-46 |
| MS.gene061044 | MS.gene36056 | -0.829962 | 3.84E-55 | -1.69E-46 |
| MS.gene06105 | MS.gene36056 | 0.804678 | 1.92E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene36056 | 0.904456 | 1.20E-79 | -1.69E-46 |
| MS.gene061200 | MS.gene36056 | 0.817194 | 3.74E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36056.t1 | MTR_8g061130 | 97.279 | 147 | 3 | 1 | 1 | 146 | 1 | 147 | 5.74e-101 | 286 |
| MS.gene36056.t1 | MTR_0002s0770 | 73.529 | 68 | 18 | 0 | 70 | 137 | 15 | 82 | 1.74e-31 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36056.t1 | AT4G11175 | 69.014 | 71 | 22 | 0 | 67 | 137 | 67 | 137 | 1.01e-30 | 108 |
Find 68 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATGGTGGAGTGGGGTTT+AGG | 0.216308 | 8.1:+52707286 | None:intergenic |
| CTAAAGGACGTATCATTTAT+AGG | 0.230954 | 8.1:-52707041 | MS.gene36056:CDS |
| GGATGGTGGAGTGGGGTTTA+GGG | 0.277606 | 8.1:+52707287 | None:intergenic |
| TTCTGCTCGCTGGCTTTGTC+GGG | 0.289706 | 8.1:+52707234 | None:intergenic |
| TTTCTGCTCGCTGGCTTTGT+CGG | 0.294784 | 8.1:+52707233 | None:intergenic |
| ATAAATGATACGTCCTTTAG+AGG | 0.337699 | 8.1:+52707044 | None:intergenic |
| ACAAAGCCAGCGAGCAGAAA+TGG | 0.346149 | 8.1:-52707230 | MS.gene36056:CDS |
| GTTGGCGATGGTGAATGATT+GGG | 0.370868 | 8.1:+52707394 | None:intergenic |
| GATGGAGGGCGGAGGAAGGA+TGG | 0.371912 | 8.1:+52707312 | None:intergenic |
| TGGGGTGCGAAAGTGGAGAG+TGG | 0.374140 | 8.1:+52707332 | None:intergenic |
| GAATGATTGGGGCGTGGAAT+GGG | 0.377126 | 8.1:+52707406 | None:intergenic |
| AATTACGTTCGGATCCTTCC+TGG | 0.385097 | 8.1:-52707102 | MS.gene36056:CDS |
| TGGTGGAGTGGGGTTTAGGG+GGG | 0.388729 | 8.1:+52707290 | None:intergenic |
| GATGGTGGAGTGGGGTTTAG+GGG | 0.391934 | 8.1:+52707288 | None:intergenic |
| ATGGAGGGCGGAGGAAGGAT+GGG | 0.406506 | 8.1:+52707313 | None:intergenic |
| ATGGTGGAGTGGGGTTTAGG+GGG | 0.407363 | 8.1:+52707289 | None:intergenic |
| GGTTGGCGATGGTGAATGAT+TGG | 0.410559 | 8.1:+52707393 | None:intergenic |
| CGAACACGGAACATGCCGTT+AGG | 0.422494 | 8.1:+52707177 | None:intergenic |
| GGATCCGAACGTAATTCTTT+CGG | 0.427721 | 8.1:+52707109 | None:intergenic |
| CTTATCCTTGGATATATCTC+TGG | 0.434923 | 8.1:-52707138 | MS.gene36056:CDS |
| TGGAGGGCGGAGGAAGGATG+GGG | 0.435398 | 8.1:+52707314 | None:intergenic |
| GTGAAGATGGATGGTGGAGT+GGG | 0.440957 | 8.1:+52707279 | None:intergenic |
| GTGGACCCATTTCTGCTCGC+TGG | 0.449738 | 8.1:+52707224 | None:intergenic |
| GGAAGGATGGGGTGCGAAAG+TGG | 0.455893 | 8.1:+52707325 | None:intergenic |
| TGAATGATTGGGGCGTGGAA+TGG | 0.456507 | 8.1:+52707405 | None:intergenic |
| GGGGTGCGAAAGTGGAGAGT+GGG | 0.461179 | 8.1:+52707333 | None:intergenic |
| GAGTGGGTGGTGACGGTAGA+GGG | 0.464511 | 8.1:+52707372 | None:intergenic |
| CTTGGCGGCGACGGTGAAGA+TGG | 0.464539 | 8.1:+52707266 | None:intergenic |
| CAGAGATATATCCAAGGATA+AGG | 0.465359 | 8.1:+52707139 | None:intergenic |
| GGGTGCGAAAGTGGAGAGTG+GGG | 0.467038 | 8.1:+52707334 | None:intergenic |
| CAAAGCCAGCGAGCAGAAAT+GGG | 0.476852 | 8.1:-52707229 | MS.gene36056:CDS |
| AGTCGATATGATTCCTCTAA+AGG | 0.477088 | 8.1:-52707057 | MS.gene36056:CDS |
| GATAATGAAGACCTTATCCT+TGG | 0.484697 | 8.1:-52707150 | MS.gene36056:CDS |
| TATAGGCTTCGTAATACTCC+TGG | 0.487758 | 8.1:-52707024 | MS.gene36056:CDS |
| GGTTGAGGACTTGGCGGCGA+CGG | 0.489028 | 8.1:+52707257 | None:intergenic |
| GATTGGGGCGTGGAATGGGA+TGG | 0.497058 | 8.1:+52707410 | None:intergenic |
| GGAGTGGGTGGTGACGGTAG+AGG | 0.509085 | 8.1:+52707371 | None:intergenic |
| TCTGCTCGCTGGCTTTGTCG+GGG | 0.517091 | 8.1:+52707235 | None:intergenic |
| AGAAAAGGAGTGGGTGGTGA+CGG | 0.521019 | 8.1:+52707365 | None:intergenic |
| CTGCTCGCTGGCTTTGTCGG+GGG | 0.526210 | 8.1:+52707236 | None:intergenic |
| GGTGAAGATGGATGGTGGAG+TGG | 0.532605 | 8.1:+52707278 | None:intergenic |
| AACTAAGAAGAGCCACCACC+AGG | 0.533810 | 8.1:+52707006 | None:intergenic |
| CTTCGTAATACTCCTGGTGG+TGG | 0.553297 | 8.1:-52707018 | MS.gene36056:CDS |
| GGGTGGTGACGGTAGAGGGT+TGG | 0.556218 | 8.1:+52707376 | None:intergenic |
| TTGGCGATGGTGAATGATTG+GGG | 0.557416 | 8.1:+52707395 | None:intergenic |
| TGAAGATGGATGGTGGAGTG+GGG | 0.567905 | 8.1:+52707280 | None:intergenic |
| GTGGAATGGGATGGAAGATG+AGG | 0.567978 | 8.1:+52707419 | None:intergenic |
| GCGGCGACGGTGAAGATGGA+TGG | 0.571559 | 8.1:+52707270 | None:intergenic |
| GGATCCGAAAGAATTACGTT+CGG | 0.574169 | 8.1:-52707113 | MS.gene36056:CDS |
| CACGGAACATGCCGTTAGGG+AGG | 0.578464 | 8.1:+52707181 | None:intergenic |
| GAACACGGAACATGCCGTTA+GGG | 0.578793 | 8.1:+52707178 | None:intergenic |
| CTTCATTATCGAGACGAACA+CGG | 0.580715 | 8.1:+52707163 | None:intergenic |
| TCAACTCTAACTCTATCTCC+AGG | 0.592855 | 8.1:+52707084 | None:intergenic |
| ATCACAGAATCCCTCCCTAA+CGG | 0.596048 | 8.1:-52707192 | MS.gene36056:CDS |
| GGTGCGAAAGTGGAGAGTGG+GGG | 0.609773 | 8.1:+52707335 | None:intergenic |
| TGACGGTAGAGGGTTGGCGA+TGG | 0.612664 | 8.1:+52707382 | None:intergenic |
| CTCTAACTCTATCTCCAGGA+AGG | 0.615980 | 8.1:+52707088 | None:intergenic |
| GGGTGTGGAAAGAAAAGGAG+TGG | 0.621177 | 8.1:+52707355 | None:intergenic |
| GCGACGGTGAAGATGGATGG+TGG | 0.622288 | 8.1:+52707273 | None:intergenic |
| GTGGAAAGAAAAGGAGTGGG+TGG | 0.622441 | 8.1:+52707359 | None:intergenic |
| ATTCTGTGATGAGACCTTCG+TGG | 0.627359 | 8.1:+52707205 | None:intergenic |
| ACGGAACATGCCGTTAGGGA+GGG | 0.631389 | 8.1:+52707182 | None:intergenic |
| GATGGTGAATGATTGGGGCG+TGG | 0.638062 | 8.1:+52707400 | None:intergenic |
| TCCTTGGATATATCTCTGGA+AGG | 0.641053 | 8.1:-52707134 | MS.gene36056:CDS |
| TCCTTCCAGAGATATATCCA+AGG | 0.654970 | 8.1:+52707133 | None:intergenic |
| GGTGTGGAAAGAAAAGGAGT+GGG | 0.666040 | 8.1:+52707356 | None:intergenic |
| AGGCTTCGTAATACTCCTGG+TGG | 0.702905 | 8.1:-52707021 | MS.gene36056:CDS |
| GAGCAGAAATGGGTCCACGA+AGG | 0.710770 | 8.1:-52707219 | MS.gene36056:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAAATGATACGTCCTTTAG+AGG | + | chr8.1:52707393-52707412 | None:intergenic | 30.0% | |
| CTAAAGGACGTATCATTTAT+AGG | - | chr8.1:52707393-52707412 | MS.gene36056:CDS | 30.0% | |
| AGTCGATATGATTCCTCTAA+AGG | - | chr8.1:52707377-52707396 | MS.gene36056:CDS | 35.0% | |
| CAGAGATATATCCAAGGATA+AGG | + | chr8.1:52707298-52707317 | None:intergenic | 35.0% | |
| CTTATCCTTGGATATATCTC+TGG | - | chr8.1:52707296-52707315 | MS.gene36056:CDS | 35.0% | |
| GATAATGAAGACCTTATCCT+TGG | - | chr8.1:52707284-52707303 | MS.gene36056:CDS | 35.0% | |
| CTTCATTATCGAGACGAACA+CGG | + | chr8.1:52707274-52707293 | None:intergenic | 40.0% | |
| GGATCCGAAAGAATTACGTT+CGG | - | chr8.1:52707321-52707340 | MS.gene36056:CDS | 40.0% | |
| GGATCCGAACGTAATTCTTT+CGG | + | chr8.1:52707328-52707347 | None:intergenic | 40.0% | |
| TATAGGCTTCGTAATACTCC+TGG | - | chr8.1:52707410-52707429 | MS.gene36056:CDS | 40.0% | |
| TCAACTCTAACTCTATCTCC+AGG | + | chr8.1:52707353-52707372 | None:intergenic | 40.0% | |
| TCCTTCCAGAGATATATCCA+AGG | + | chr8.1:52707304-52707323 | None:intergenic | 40.0% | |
| TCCTTGGATATATCTCTGGA+AGG | - | chr8.1:52707300-52707319 | MS.gene36056:CDS | 40.0% | |
| AATTACGTTCGGATCCTTCC+TGG | - | chr8.1:52707332-52707351 | MS.gene36056:CDS | 45.0% | |
| ATCACAGAATCCCTCCCTAA+CGG | - | chr8.1:52707242-52707261 | MS.gene36056:CDS | 45.0% | |
| ATTCTGTGATGAGACCTTCG+TGG | + | chr8.1:52707232-52707251 | None:intergenic | 45.0% | |
| CTCTAACTCTATCTCCAGGA+AGG | + | chr8.1:52707349-52707368 | None:intergenic | 45.0% | |
| GGTGTGGAAAGAAAAGGAGT+GGG | + | chr8.1:52707081-52707100 | None:intergenic | 45.0% | |
| GTTGGCGATGGTGAATGATT+GGG | + | chr8.1:52707043-52707062 | None:intergenic | 45.0% | |
| TTGGCGATGGTGAATGATTG+GGG | + | chr8.1:52707042-52707061 | None:intergenic | 45.0% | |
| ACAAAGCCAGCGAGCAGAAA+TGG | - | chr8.1:52707204-52707223 | MS.gene36056:CDS | 50.0% | |
| AGAAAAGGAGTGGGTGGTGA+CGG | + | chr8.1:52707072-52707091 | None:intergenic | 50.0% | |
| AGGCTTCGTAATACTCCTGG+TGG | - | chr8.1:52707413-52707432 | MS.gene36056:CDS | 50.0% | |
| CAAAGCCAGCGAGCAGAAAT+GGG | - | chr8.1:52707205-52707224 | MS.gene36056:CDS | 50.0% | |
| CTTCGTAATACTCCTGGTGG+TGG | - | chr8.1:52707416-52707435 | MS.gene36056:CDS | 50.0% | |
| GAACACGGAACATGCCGTTA+GGG | + | chr8.1:52707259-52707278 | None:intergenic | 50.0% | |
| GAATGATTGGGGCGTGGAAT+GGG | + | chr8.1:52707031-52707050 | None:intergenic | 50.0% | |
| GGGTGTGGAAAGAAAAGGAG+TGG | + | chr8.1:52707082-52707101 | None:intergenic | 50.0% | |
| GGTTGGCGATGGTGAATGAT+TGG | + | chr8.1:52707044-52707063 | None:intergenic | 50.0% | |
| GTGGAAAGAAAAGGAGTGGG+TGG | + | chr8.1:52707078-52707097 | None:intergenic | 50.0% | |
| GTGGAATGGGATGGAAGATG+AGG | + | chr8.1:52707018-52707037 | None:intergenic | 50.0% | |
| TGAATGATTGGGGCGTGGAA+TGG | + | chr8.1:52707032-52707051 | None:intergenic | 50.0% | |
| ! | AGTGGGGGTGTGGAAAGAAA+AGG | + | chr8.1:52707087-52707106 | None:intergenic | 50.0% |
| !! | GTGAAGATGGATGGTGGAGT+GGG | + | chr8.1:52707158-52707177 | None:intergenic | 50.0% |
| !! | TGAAGATGGATGGTGGAGTG+GGG | + | chr8.1:52707157-52707176 | None:intergenic | 50.0% |
| !! | TTTCTGCTCGCTGGCTTTGT+CGG | + | chr8.1:52707204-52707223 | None:intergenic | 50.0% |
| ACGGAACATGCCGTTAGGGA+GGG | + | chr8.1:52707255-52707274 | None:intergenic | 55.0% | |
| CGAACACGGAACATGCCGTT+AGG | + | chr8.1:52707260-52707279 | None:intergenic | 55.0% | |
| GAGCAGAAATGGGTCCACGA+AGG | - | chr8.1:52707215-52707234 | MS.gene36056:CDS | 55.0% | |
| GATGGTGAATGATTGGGGCG+TGG | + | chr8.1:52707037-52707056 | None:intergenic | 55.0% | |
| TTTGTCGGGGGTTGAGGACT+TGG | + | chr8.1:52707189-52707208 | None:intergenic | 55.0% | |
| ! | ATGGTGGAGTGGGGTTTAGG+GGG | + | chr8.1:52707148-52707167 | None:intergenic | 55.0% |
| !! | GATGGTGGAGTGGGGTTTAG+GGG | + | chr8.1:52707149-52707168 | None:intergenic | 55.0% |
| !! | GGATGGTGGAGTGGGGTTTA+GGG | + | chr8.1:52707150-52707169 | None:intergenic | 55.0% |
| !! | GGTGAAGATGGATGGTGGAG+TGG | + | chr8.1:52707159-52707178 | None:intergenic | 55.0% |
| !! | TGGATGGTGGAGTGGGGTTT+AGG | + | chr8.1:52707151-52707170 | None:intergenic | 55.0% |
| !! | TTCTGCTCGCTGGCTTTGTC+GGG | + | chr8.1:52707203-52707222 | None:intergenic | 55.0% |
| ATGGAGGGCGGAGGAAGGAT+GGG | + | chr8.1:52707124-52707143 | None:intergenic | 60.0% | |
| CACGGAACATGCCGTTAGGG+AGG | + | chr8.1:52707256-52707275 | None:intergenic | 60.0% | |
| GAAAGTGGAGAGTGGGGGTG+TGG | + | chr8.1:52707097-52707116 | None:intergenic | 60.0% | |
| GATTGGGGCGTGGAATGGGA+TGG | + | chr8.1:52707027-52707046 | None:intergenic | 60.0% | |
| GCGACGGTGAAGATGGATGG+TGG | + | chr8.1:52707164-52707183 | None:intergenic | 60.0% | |
| GGAAGGATGGGGTGCGAAAG+TGG | + | chr8.1:52707112-52707131 | None:intergenic | 60.0% | |
| GGGGTGCGAAAGTGGAGAGT+GGG | + | chr8.1:52707104-52707123 | None:intergenic | 60.0% | |
| GGGTGCGAAAGTGGAGAGTG+GGG | + | chr8.1:52707103-52707122 | None:intergenic | 60.0% | |
| GGTGCGAAAGTGGAGAGTGG+GGG | + | chr8.1:52707102-52707121 | None:intergenic | 60.0% | |
| GTGGACCCATTTCTGCTCGC+TGG | + | chr8.1:52707213-52707232 | None:intergenic | 60.0% | |
| TGGGGTGCGAAAGTGGAGAG+TGG | + | chr8.1:52707105-52707124 | None:intergenic | 60.0% | |
| TGGGGTTTAGGGGGGATGGA+GGG | + | chr8.1:52707139-52707158 | None:intergenic | 60.0% | |
| TGGTGGAGTGGGGTTTAGGG+GGG | + | chr8.1:52707147-52707166 | None:intergenic | 60.0% | |
| ! | TGACGGTAGAGGGTTGGCGA+TGG | + | chr8.1:52707055-52707074 | None:intergenic | 60.0% |
| !! | GAGTGGGTGGTGACGGTAGA+GGG | + | chr8.1:52707065-52707084 | None:intergenic | 60.0% |
| !! | TCTGCTCGCTGGCTTTGTCG+GGG | + | chr8.1:52707202-52707221 | None:intergenic | 60.0% |
| CTTGGCGGCGACGGTGAAGA+TGG | + | chr8.1:52707171-52707190 | None:intergenic | 65.0% | |
| GATGGAGGGCGGAGGAAGGA+TGG | + | chr8.1:52707125-52707144 | None:intergenic | 65.0% | |
| GCGGCGACGGTGAAGATGGA+TGG | + | chr8.1:52707167-52707186 | None:intergenic | 65.0% | |
| GGAGTGGGGTTTAGGGGGGA+TGG | + | chr8.1:52707143-52707162 | None:intergenic | 65.0% | |
| GGTTGAGGACTTGGCGGCGA+CGG | + | chr8.1:52707180-52707199 | None:intergenic | 65.0% | |
| GGTTTAGGGGGGATGGAGGG+CGG | + | chr8.1:52707136-52707155 | None:intergenic | 65.0% | |
| GTCGGGGGTTGAGGACTTGG+CGG | + | chr8.1:52707186-52707205 | None:intergenic | 65.0% | |
| GTGGGGTTTAGGGGGGATGG+AGG | + | chr8.1:52707140-52707159 | None:intergenic | 65.0% | |
| TGGAGGGCGGAGGAAGGATG+GGG | + | chr8.1:52707123-52707142 | None:intergenic | 65.0% | |
| !! | CTGCTCGCTGGCTTTGTCGG+GGG | + | chr8.1:52707201-52707220 | None:intergenic | 65.0% |
| !! | GCTGGCTTTGTCGGGGGTTG+AGG | + | chr8.1:52707195-52707214 | None:intergenic | 65.0% |
| !! | GGAGTGGGTGGTGACGGTAG+AGG | + | chr8.1:52707066-52707085 | None:intergenic | 65.0% |
| !! | GGGTGGTGACGGTAGAGGGT+TGG | + | chr8.1:52707061-52707080 | None:intergenic | 65.0% |
| TTAGGGGGGATGGAGGGCGG+AGG | + | chr8.1:52707133-52707152 | None:intergenic | 70.0% | |
| GGGGGATGGAGGGCGGAGGA+AGG | + | chr8.1:52707129-52707148 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 52707008 | 52707448 | 52707008 | ID=MS.gene36056 |
| chr8.1 | mRNA | 52707008 | 52707448 | 52707008 | ID=MS.gene36056.t1;Parent=MS.gene36056 |
| chr8.1 | exon | 52707008 | 52707448 | 52707008 | ID=MS.gene36056.t1.exon1;Parent=MS.gene36056.t1 |
| chr8.1 | CDS | 52707008 | 52707448 | 52707008 | ID=cds.MS.gene36056.t1;Parent=MS.gene36056.t1 |
| Gene Sequence |
| Protein sequence |
