AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0180120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180120 MtrunA17_Chr1g0206091 100.000 96 0 0 1 96 1 96 1.73e-67 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180120 AT1G61780.1 88.776 98 9 1 1 96 1 98 7.77e-60 178

Find 45 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATCTTGGATATTCAAAT+TGG 0.227939 1_4:+67895144 Msa0180120:five_prime_UTR
GCACCTTCTTTCCATTTATC+TGG 0.231971 1_4:-67895363 None:intergenic
CAATTTGAATATCCAAGATT+TGG 0.257745 1_4:-67895143 None:intergenic
TGAAGGGAACCTGTTCTTCT+TGG 0.293706 1_4:-67895439 None:intergenic
AGGTGATAGTACCAGATAAA+TGG 0.304949 1_4:+67895352 Msa0180120:CDS
CCCCTCCCTCTCATAAGTTT+TGG 0.330996 1_4:-67898858 None:intergenic
AATTAGCTGAAAAGGGGTAT+AGG 0.350950 1_4:+67895112 Msa0180120:five_prime_UTR
GAAGGCTGTCGACGTCGTTT+TGG 0.355325 1_4:+67895053 Msa0180120:five_prime_UTR
ATTAGCTGAAAAGGGGTATA+GGG 0.371633 1_4:+67895113 Msa0180120:five_prime_UTR
TTAGACTATATTATATATAA+AGG 0.375911 1_4:-67898646 None:intergenic
TATGAGAGGGAGGGGCGTTT+TGG 0.376336 1_4:+67898866 Msa0180120:three_prime_UTR
AACTCCTTTCCAAGAAGAAC+AGG 0.405714 1_4:+67895430 Msa0180120:CDS
ACGACACGAATTAGCTGAAA+AGG 0.416736 1_4:+67895104 Msa0180120:five_prime_UTR
AGAGGAACCTTACGTCTTGC+CGG 0.429446 1_4:-67898898 None:intergenic
CCTTGTAGATGGACCCCTTA+CGG 0.465158 1_4:+67898343 Msa0180120:intron
GTTGCTTTGTTTATAAAACT+TGG 0.466176 1_4:-67898569 None:intergenic
CACACGTGTGCTTACTCAAA+AGG 0.468563 1_4:+67898421 Msa0180120:CDS
TGCACTTGGTTGTTCCGTAA+GGG 0.477894 1_4:-67898357 None:intergenic
ATGCACTTGGTTGTTCCGTA+AGG 0.478636 1_4:-67898358 None:intergenic
CTGCTTACAAATGATGCACT+TGG 0.484104 1_4:-67898371 None:intergenic
CGACACGAATTAGCTGAAAA+GGG 0.490307 1_4:+67895105 Msa0180120:five_prime_UTR
CACTTTGATCAATAGCATAG+AGG 0.500824 1_4:-67898916 None:intergenic
ATAAACAAAGCAACGTGTAG+TGG 0.523993 1_4:+67898578 Msa0180120:CDS
TTGTAAGCAGCAAGTGCACC+AGG 0.524349 1_4:+67898384 Msa0180120:CDS
GTACCAGATAAATGGAAAGA+AGG 0.526643 1_4:+67895360 Msa0180120:CDS
CCAAAACTTATGAGAGGGAG+GGG 0.533985 1_4:+67898858 Msa0180120:three_prime_UTR
GCACTTGGTTGTTCCGTAAG+GGG 0.542962 1_4:-67898356 None:intergenic
TGTGACAGTACTTGCCATCC+TGG 0.544753 1_4:-67898402 None:intergenic
GGAACCTGTTCTTCTTGGAA+AGG 0.545948 1_4:-67895434 None:intergenic
GATGAATCCGGCAAGACGTA+AGG 0.551859 1_4:+67898891 Msa0180120:three_prime_UTR
ACCAAAACTTATGAGAGGGA+GGG 0.578094 1_4:+67898857 Msa0180120:three_prime_UTR
TTTGACCAAAACTTATGAGA+GGG 0.582502 1_4:+67898853 Msa0180120:three_prime_UTR
GACCAAAACTTATGAGAGGG+AGG 0.596320 1_4:+67898856 Msa0180120:three_prime_UTR
TAGATCTCATAAATACTCGA+AGG 0.603857 1_4:+67895035 Msa0180120:five_prime_UTR
ATTTGACCAAAACTTATGAG+AGG 0.607189 1_4:+67898852 Msa0180120:three_prime_UTR
AGCAATACTACTGAGGGTGG+TGG 0.611332 1_4:+67895387 Msa0180120:CDS
AATTTGAGTTGATCTTAGCA+AGG 0.619178 1_4:-67898832 None:intergenic
GGTGCAAGCAATACTACTGA+GGG 0.634401 1_4:+67895381 Msa0180120:CDS
AAGCAGCAAGTGCACCAGGA+TGG 0.635149 1_4:+67898388 Msa0180120:CDS
AGAATCATTAGCGACTGCGA+TGG 0.640288 1_4:+67895206 Msa0180120:exon
GCAGGAGTCTGTGCGATGTG+TGG 0.652505 1_4:+67898529 Msa0180120:intron
GCAAGCAATACTACTGAGGG+TGG 0.654071 1_4:+67895384 Msa0180120:CDS
GACACGAATTAGCTGAAAAG+GGG 0.659143 1_4:+67895106 Msa0180120:five_prime_UTR
AGGTCAGAAGAAATTATCGA+AGG 0.666813 1_4:+67895332 Msa0180120:intron
AGGTGCAAGCAATACTACTG+AGG 0.668804 1_4:+67895380 Msa0180120:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATTTATTTTTTTAATT+AGG + chr1_4:67895475-67895494 Msa0180120:intron 0.0%
!!! ATTATTTATTTTTTTAATTA+GGG + chr1_4:67895476-67895495 Msa0180120:intron 0.0%
!!! AATAATTTTAGTTTTGATAT+TGG + chr1_4:67895571-67895590 Msa0180120:intron 10.0%
!!! AAAAAGTAAATAAATTTTAG+GGG - chr1_4:67896583-67896602 None:intergenic 10.0%
!!! GAAAAAGTAAATAAATTTTA+GGG - chr1_4:67896584-67896603 None:intergenic 10.0%
!!! TGAAAAAGTAAATAAATTTT+AGG - chr1_4:67896585-67896604 None:intergenic 10.0%
!!! TAAATTTTGAGTAATATTTA+AGG + chr1_4:67897544-67897563 Msa0180120:intron 10.0%
!!! AATATATTTTTTGTATAGTA+TGG + chr1_4:67897573-67897592 Msa0180120:intron 10.0%
!! TTAGACTATATTATATATAA+AGG - chr1_4:67898649-67898668 None:intergenic 10.0%
!!! GTAAATTCAATTGAATTTTT+TGG + chr1_4:67895289-67895308 Msa0180120:intron 15.0%
!!! TTTTGAAAACAGTTATTTTT+TGG + chr1_4:67896443-67896462 Msa0180120:intron 15.0%
!!! AAAAGTAAATAAATTTTAGG+GGG - chr1_4:67896582-67896601 None:intergenic 15.0%
!! AAAAAGATAAGTAAAAAGAA+CGG - chr1_4:67897292-67897311 None:intergenic 15.0%
!! ATTTACAAAAGCTAGAAATA+TGG - chr1_4:67895275-67895294 None:intergenic 20.0%
!! AATAAATAATTGAAGGGTTT+AGG - chr1_4:67895466-67895485 None:intergenic 20.0%
!! AATTGTCAAAATTAATTCCA+CGG + chr1_4:67896044-67896063 Msa0180120:intron 20.0%
!!! TTTTATATGTAGACTTGATA+TGG + chr1_4:67896251-67896270 Msa0180120:intron 20.0%
!!! AAAGTAAATAAATTTTAGGG+GGG - chr1_4:67896581-67896600 None:intergenic 20.0%
!!! CCTGTTAAAAAATAAAGTTT+TGG - chr1_4:67896609-67896628 None:intergenic 20.0%
!!! CCAAAACTTTATTTTTTAAC+AGG + chr1_4:67896606-67896625 Msa0180120:intron 20.0%
!!! TTATTTTTTAACAGGACATA+AGG + chr1_4:67896614-67896633 Msa0180120:intron 20.0%
!! AAAGGAAAACATTTAGTATT+GGG - chr1_4:67897132-67897151 None:intergenic 20.0%
!! AAAAGGAAAACATTTAGTAT+TGG - chr1_4:67897133-67897152 None:intergenic 20.0%
!!! TATTTTTCTTTTTGAAAGAC+AGG - chr1_4:67897189-67897208 None:intergenic 20.0%
!! AATAATCTAAGTAGAAAGAA+AGG - chr1_4:67897243-67897262 None:intergenic 20.0%
!! AAGCTATTGAACATTAAAAT+TGG + chr1_4:67897779-67897798 Msa0180120:intron 20.0%
!!! ATATTTTTGATATATCTGAG+TGG + chr1_4:67897842-67897861 Msa0180120:intron 20.0%
!! TGAACATTTACAAAAAGTAA+TGG - chr1_4:67898150-67898169 None:intergenic 20.0%
!! CTGTAAAAAGTTAATATTGT+GGG + chr1_4:67898699-67898718 Msa0180120:three_prime_UTR 20.0%
! CAATTTGAATATCCAAGATT+TGG - chr1_4:67895146-67895165 None:intergenic 25.0%
! CAAATCTTGGATATTCAAAT+TGG + chr1_4:67895144-67895163 Msa0180120:five_prime_UTR 25.0%
! TAATTTGAATTCGATGTTGT+AGG + chr1_4:67895312-67895331 Msa0180120:intron 25.0%
!! GTATGAAAAGCATAGTAATT+TGG + chr1_4:67895498-67895517 Msa0180120:intron 25.0%
!! TGGTATAACTTCAAATTTTG+AGG + chr1_4:67895626-67895645 Msa0180120:intron 25.0%
!!! TATAACTTCAAATTTTGAGG+TGG + chr1_4:67895629-67895648 Msa0180120:intron 25.0%
!!! ATAACTTCAAATTTTGAGGT+GGG + chr1_4:67895630-67895649 Msa0180120:intron 25.0%
!!! ATAAGTTTTATGTGGTTGTT+CGG + chr1_4:67895708-67895727 Msa0180120:intron 25.0%
! TAGCTTATGATATTGTTCAT+AGG + chr1_4:67895746-67895765 Msa0180120:intron 25.0%
! TTAAGATAGCTTATGAAAAC+AGG + chr1_4:67895797-67895816 Msa0180120:intron 25.0%
! ATTGTCAAAATTAATTCCAC+GGG + chr1_4:67896045-67896064 Msa0180120:intron 25.0%
!! GAAAGAAACCTTATGTTTTT+AGG + chr1_4:67896296-67896315 Msa0180120:intron 25.0%
! AATAAAATGCAAGGGAAAAA+CGG - chr1_4:67896492-67896511 None:intergenic 25.0%
! TGGCATTAAATAAAATGCAA+GGG - chr1_4:67896500-67896519 None:intergenic 25.0%
! ATGGCATTAAATAAAATGCA+AGG - chr1_4:67896501-67896520 None:intergenic 25.0%
!!! TGTTTTTTTGGCTATTTTGT+GGG + chr1_4:67896884-67896903 Msa0180120:intron 25.0%
!!! AGAGTAAGGTGTTTTATAAA+AGG - chr1_4:67897150-67897169 None:intergenic 25.0%
!! ATGTTCAATAGCTTTCTTTT+TGG - chr1_4:67897773-67897792 None:intergenic 25.0%
!!! TGTAATTCTTGCTTTTTGAA+TGG + chr1_4:67898022-67898041 Msa0180120:intron 25.0%
! ACAGATCAAAGTATAGTAAT+TGG - chr1_4:67898228-67898247 None:intergenic 25.0%
! CAAATTACAAAAGGAGAAAA+GGG - chr1_4:67898264-67898283 None:intergenic 25.0%
! TCAAATTACAAAAGGAGAAA+AGG - chr1_4:67898265-67898284 None:intergenic 25.0%
! TTGAATTTATTCCTTGTAGA+TGG + chr1_4:67898332-67898351 Msa0180120:intron 25.0%
!!! GTTGCTTTGTTTATAAAACT+TGG - chr1_4:67898572-67898591 None:intergenic 25.0%
! GCTGTAAAAAGTTAATATTG+TGG + chr1_4:67898698-67898717 Msa0180120:three_prime_UTR 25.0%
!!! ACAATAGTGTTTTTTCGATA+AGG - chr1_4:67898760-67898779 None:intergenic 25.0%
! TATCGAAAAAACACTATTGT+TGG + chr1_4:67898760-67898779 Msa0180120:three_prime_UTR 25.0%
TAGATCTCATAAATACTCGA+AGG + chr1_4:67895035-67895054 Msa0180120:five_prime_UTR 30.0%
ACAAAAGCTAGAAATATGGA+TGG - chr1_4:67895271-67895290 None:intergenic 30.0%
!! AGTTTTGATATTGGAAGCTT+TGG + chr1_4:67895580-67895599 Msa0180120:intron 30.0%
!! GTTTTGATATTGGAAGCTTT+GGG + chr1_4:67895581-67895600 Msa0180120:intron 30.0%
! AATTTGAAGTTATACCAAGC+AGG - chr1_4:67895623-67895642 None:intergenic 30.0%
!!! TAACTTCAAATTTTGAGGTG+GGG + chr1_4:67895631-67895650 Msa0180120:intron 30.0%
!! TAAGTTTTATGTGGTTGTTC+GGG + chr1_4:67895709-67895728 Msa0180120:intron 30.0%
! ACAACTTGTTTCCAGTTTAT+AGG - chr1_4:67895920-67895939 None:intergenic 30.0%
AGAAAATGGAACAAAAAGAG+TGG - chr1_4:67895996-67896015 None:intergenic 30.0%
! AGCATCGAGTTTAAAGAAAA+TGG - chr1_4:67896010-67896029 None:intergenic 30.0%
CCTTAAACATCAAATCCTAT+TGG - chr1_4:67896148-67896167 None:intergenic 30.0%
CCAATAGGATTTGATGTTTA+AGG + chr1_4:67896145-67896164 Msa0180120:intron 30.0%
! ACTTGATATGGTTAGTTGTA+TGG + chr1_4:67896263-67896282 Msa0180120:intron 30.0%
!!! AGAAACCTTATGTTTTTAGG+TGG + chr1_4:67896299-67896318 Msa0180120:intron 30.0%
AACTACCACCTAAAAACATA+AGG - chr1_4:67896307-67896326 None:intergenic 30.0%
TGAACAAGTGAATCAATTGT+TGG + chr1_4:67896366-67896385 Msa0180120:intron 30.0%
AATCAATTGTTGGATGTCAT+TGG + chr1_4:67896376-67896395 Msa0180120:intron 30.0%
ATCAATTGTTGGATGTCATT+GGG + chr1_4:67896377-67896396 Msa0180120:intron 30.0%
TCAAAATTGTATCAACGTAC+AGG - chr1_4:67896429-67896448 None:intergenic 30.0%
AGTGAGCATGATGAAATTAT+GGG - chr1_4:67896542-67896561 None:intergenic 30.0%
AAGTGAGCATGATGAAATTA+TGG - chr1_4:67896543-67896562 None:intergenic 30.0%
!!! GTGTTTTTTTGGCTATTTTG+TGG + chr1_4:67896883-67896902 Msa0180120:intron 30.0%
! TCTCGTTTTATATGCGTTTT+TGG - chr1_4:67896929-67896948 None:intergenic 30.0%
GAGGAATGAAGATGAAATAA+AGG + chr1_4:67896987-67897006 Msa0180120:intron 30.0%
GAAGATGAAATAAAGGACTT+TGG + chr1_4:67896994-67897013 Msa0180120:intron 30.0%
ATCAAACTAGGTTAAAGTTG+TGG + chr1_4:67897078-67897097 Msa0180120:intron 30.0%
TAAAGTTGTGGAAACTTATC+TGG + chr1_4:67897090-67897109 Msa0180120:intron 30.0%
AAAGTTGTGGAAACTTATCT+GGG + chr1_4:67897091-67897110 Msa0180120:intron 30.0%
GGTGTATTAGTAAAAGAGTA+AGG - chr1_4:67897164-67897183 None:intergenic 30.0%
!! TTTCTTTTTGAAAGACAGGT+GGG - chr1_4:67897185-67897204 None:intergenic 30.0%
!! TTTTCTTTTTGAAAGACAGG+TGG - chr1_4:67897186-67897205 None:intergenic 30.0%
TCAATTATACGTAAGACTTG+TGG + chr1_4:67897494-67897513 Msa0180120:intron 30.0%
TATATGCTAGTGTTATGTTG+TGG - chr1_4:67897727-67897746 None:intergenic 30.0%
TGGAATGGAAAATGTTAGTA+TGG - chr1_4:67897753-67897772 None:intergenic 30.0%
!! CAATAGCTTTCTTTTTGGAA+TGG - chr1_4:67897768-67897787 None:intergenic 30.0%
ACAATGTAACCAAAACAGTT+CGG - chr1_4:67897877-67897896 None:intergenic 30.0%
AGACATATCATGATGAGATA+TGG - chr1_4:67897913-67897932 None:intergenic 30.0%
GCGCTTATATTTAGTAATCA+CGG + chr1_4:67897992-67898011 Msa0180120:intron 30.0%
! GTTTAGAATGAGTGATGTTA+TGG + chr1_4:67898077-67898096 Msa0180120:intron 30.0%
!! ACTTTGATCTGTTGTTTCAA+TGG + chr1_4:67898236-67898255 Msa0180120:intron 30.0%
!! CTTTGATCTGTTGTTTCAAT+GGG + chr1_4:67898237-67898256 Msa0180120:intron 30.0%
GGTGTGTATCAAATTACAAA+AGG - chr1_4:67898273-67898292 None:intergenic 30.0%
GTTCACATAACATAAAACCA+GGG - chr1_4:67898294-67898313 None:intergenic 30.0%
TGTTCACATAACATAAAACC+AGG - chr1_4:67898295-67898314 None:intergenic 30.0%
!! AATTTGAGTTGATCTTAGCA+AGG - chr1_4:67898835-67898854 None:intergenic 30.0%
ATTTGACCAAAACTTATGAG+AGG + chr1_4:67898852-67898871 Msa0180120:three_prime_UTR 30.0%
TTTGACCAAAACTTATGAGA+GGG + chr1_4:67898853-67898872 Msa0180120:three_prime_UTR 30.0%
!! CGTTTTGAGCTTTTGTTTAC+AGG - chr1_4:67895087-67895106 None:intergenic 35.0%
! AATTAGCTGAAAAGGGGTAT+AGG + chr1_4:67895112-67895131 Msa0180120:five_prime_UTR 35.0%
! ATTAGCTGAAAAGGGGTATA+GGG + chr1_4:67895113-67895132 Msa0180120:five_prime_UTR 35.0%
!! TAGGGTTTTGATCCAAATCT+TGG + chr1_4:67895131-67895150 Msa0180120:five_prime_UTR 35.0%
TATGGATGGATGGAAGAAAA+AGG - chr1_4:67895257-67895276 None:intergenic 35.0%
AAGCTAGAAATATGGATGGA+TGG - chr1_4:67895267-67895286 None:intergenic 35.0%
AGGTCAGAAGAAATTATCGA+AGG + chr1_4:67895332-67895351 Msa0180120:intron 35.0%
AGGTGATAGTACCAGATAAA+TGG + chr1_4:67895352-67895371 Msa0180120:CDS 35.0%
GTACCAGATAAATGGAAAGA+AGG + chr1_4:67895360-67895379 Msa0180120:CDS 35.0%
!!! GTTTGTTCTCGTTGATTTTG+CGG - chr1_4:67895413-67895432 None:intergenic 35.0%
!! ATTGAAGGGTTTAGGTTGAA+GGG - chr1_4:67895458-67895477 None:intergenic 35.0%
!! AATTGAAGGGTTTAGGTTGA+AGG - chr1_4:67895459-67895478 None:intergenic 35.0%
! GCAATTTTCTTTTACCTGCT+TGG + chr1_4:67895606-67895625 Msa0180120:intron 35.0%
!! TTTTGGATTATTGGCCTGTT+TGG + chr1_4:67895656-67895675 Msa0180120:intron 35.0%
!! CTAGTGGCATAAGTTTTATG+TGG + chr1_4:67895700-67895719 Msa0180120:intron 35.0%
TTGTGCTATAGCCTATAAAC+TGG + chr1_4:67895906-67895925 Msa0180120:intron 35.0%
ATCAAATCCTATTGGTCCAT+GGG - chr1_4:67896140-67896159 None:intergenic 35.0%
AAGGATGTGCTATGCATATT+TGG + chr1_4:67896164-67896183 Msa0180120:intron 35.0%
!! TAAATTTTAGGGGGGTAACA+TGG - chr1_4:67896573-67896592 None:intergenic 35.0%
GTAGAAAGAGAAAGAGAGAA+AGG + chr1_4:67896962-67896981 Msa0180120:intron 35.0%
!!! ATTTTGATTGATGTCGCTCT+TGG + chr1_4:67897816-67897835 Msa0180120:intron 35.0%
!! TTTTGATTGATGTCGCTCTT+GGG + chr1_4:67897817-67897836 Msa0180120:intron 35.0%
! TTCAAACTTCCGAACTGTTT+TGG + chr1_4:67897865-67897884 Msa0180120:intron 35.0%
AATATCTGTCCAAACTGTCA+AGG + chr1_4:67898048-67898067 Msa0180120:intron 35.0%
CACATGATTCTCAATACCAT+TGG + chr1_4:67898190-67898209 Msa0180120:intron 35.0%
TTGGACAAAAAACTTGCCAA+TGG - chr1_4:67898209-67898228 None:intergenic 35.0%
TTGTAATTTGATACACACCC+TGG + chr1_4:67898274-67898293 Msa0180120:intron 35.0%
CCTGCAAATACAAAAAGATC+AGG - chr1_4:67898514-67898533 None:intergenic 35.0%
! CCTGATCTTTTTGTATTTGC+AGG + chr1_4:67898511-67898530 Msa0180120:intron 35.0%
ATAAACAAAGCAACGTGTAG+TGG + chr1_4:67898578-67898597 Msa0180120:CDS 35.0%
! TGCATTTTGCATCTTCTTCT+AGG + chr1_4:67898609-67898628 Msa0180120:three_prime_UTR 35.0%
CACTTTGATCAATAGCATAG+AGG - chr1_4:67898919-67898938 None:intergenic 35.0%
ACGACACGAATTAGCTGAAA+AGG + chr1_4:67895104-67895123 Msa0180120:five_prime_UTR 40.0%
CGACACGAATTAGCTGAAAA+GGG + chr1_4:67895105-67895124 Msa0180120:five_prime_UTR 40.0%
GACACGAATTAGCTGAAAAG+GGG + chr1_4:67895106-67895125 Msa0180120:five_prime_UTR 40.0%
GCACCTTCTTTCCATTTATC+TGG - chr1_4:67895366-67895385 None:intergenic 40.0%
AACTCCTTTCCAAGAAGAAC+AGG + chr1_4:67895430-67895449 Msa0180120:CDS 40.0%
!!! AATTTTGAGGTGGGGTGTTT+TGG + chr1_4:67895639-67895658 Msa0180120:intron 40.0%
CAGGTAAGTCAAATCCAAAC+AGG - chr1_4:67895673-67895692 None:intergenic 40.0%
ACTTACCTGAGCTTATCTAG+TGG + chr1_4:67895684-67895703 Msa0180120:intron 40.0%
TTATGCCACTAGATAAGCTC+AGG - chr1_4:67895692-67895711 None:intergenic 40.0%
!! CGTGAATTTTAGGCACTGTT+TGG - chr1_4:67895947-67895966 None:intergenic 40.0%
ATGGAACAAAAAGAGTGGCA+TGG - chr1_4:67895991-67896010 None:intergenic 40.0%
!! CTGCCATTTCCTACTTTTGT+GGG - chr1_4:67896090-67896109 None:intergenic 40.0%
!! TCTGCCATTTCCTACTTTTG+TGG - chr1_4:67896091-67896110 None:intergenic 40.0%
ATGGCAGATTTGAATCTGAC+AGG + chr1_4:67896103-67896122 Msa0180120:intron 40.0%
TGGCAGATTTGAATCTGACA+GGG + chr1_4:67896104-67896123 Msa0180120:intron 40.0%
TCAAATCCTATTGGTCCATG+GGG - chr1_4:67896139-67896158 None:intergenic 40.0%
CATCAAATCCTATTGGTCCA+TGG - chr1_4:67896141-67896160 None:intergenic 40.0%
! TTAATGCCATTTACGTGCTG+TGG + chr1_4:67896511-67896530 Msa0180120:intron 40.0%
! AGGACATAAGGTTGACTTCA+TGG + chr1_4:67896626-67896645 Msa0180120:intron 40.0%
! TAAGGTTGACTTCATGGTTG+AGG + chr1_4:67896632-67896651 Msa0180120:intron 40.0%
AGAGAAAGAGAGAAAGGATG+AGG + chr1_4:67896968-67896987 Msa0180120:intron 40.0%
CAGATCTGAAAGAAGAAGAC+TGG + chr1_4:67897030-67897049 Msa0180120:intron 40.0%
AAGAAGAAGAAGAAGCTGTG+CGG + chr1_4:67897053-67897072 Msa0180120:intron 40.0%
!!! TTTTTTGAGATGCGAGTTGC+AGG + chr1_4:67897439-67897458 Msa0180120:intron 40.0%
TGCTAGTGTTATGTTGTGGA+TGG - chr1_4:67897723-67897742 None:intergenic 40.0%
! TGTCCAAACTGTCAAGGTTT+TGG + chr1_4:67898054-67898073 Msa0180120:intron 40.0%
! GTCCAAACTGTCAAGGTTTT+GGG + chr1_4:67898055-67898074 Msa0180120:intron 40.0%
AACCCAAAACCTTGACAGTT+TGG - chr1_4:67898060-67898079 None:intergenic 40.0%
AGTACATGACACTTGTAGGA+GGG + chr1_4:67898119-67898138 Msa0180120:intron 40.0%
CTGCTTACAAATGATGCACT+TGG - chr1_4:67898374-67898393 None:intergenic 40.0%
ACCAAAACTTATGAGAGGGA+GGG + chr1_4:67898857-67898876 Msa0180120:three_prime_UTR 40.0%
AGAATCATTAGCGACTGCGA+TGG + chr1_4:67895206-67895225 Msa0180120:exon 45.0%
AGGTGCAAGCAATACTACTG+AGG + chr1_4:67895380-67895399 Msa0180120:CDS 45.0%
GGTGCAAGCAATACTACTGA+GGG + chr1_4:67895381-67895400 Msa0180120:CDS 45.0%
GGAACCTGTTCTTCTTGGAA+AGG - chr1_4:67895437-67895456 None:intergenic 45.0%
TGAAGGGAACCTGTTCTTCT+TGG - chr1_4:67895442-67895461 None:intergenic 45.0%
!! GGTGGGGTGTTTTGGATTAT+TGG + chr1_4:67895647-67895666 Msa0180120:intron 45.0%
! TACCACGCAGCGTGAATTTT+AGG - chr1_4:67895957-67895976 None:intergenic 45.0%
! TACAGTTGATTCAAGACCCG+TGG - chr1_4:67896064-67896083 None:intergenic 45.0%
ACTGTACCTCCCACAAAAGT+AGG + chr1_4:67896078-67896097 Msa0180120:intron 45.0%
!! CCATTTCCTACTTTTGTGGG+AGG - chr1_4:67896087-67896106 None:intergenic 45.0%
CCTCCCACAAAAGTAGGAAA+TGG + chr1_4:67896084-67896103 Msa0180120:intron 45.0%
GAGAATCCACAGCACGTAAA+TGG - chr1_4:67896520-67896539 None:intergenic 45.0%
TTAGGGGGGTAACATGGAAA+GGG - chr1_4:67896567-67896586 None:intergenic 45.0%
TTTAGGGGGGTAACATGGAA+AGG - chr1_4:67896568-67896587 None:intergenic 45.0%
! GTTGACTTCATGGTTGAGGT+GGG + chr1_4:67896636-67896655 Msa0180120:intron 45.0%
TTTGACAACAGAGGTGGATG+GGG - chr1_4:67896709-67896728 None:intergenic 45.0%
GTTTGACAACAGAGGTGGAT+GGG - chr1_4:67896710-67896729 None:intergenic 45.0%
!!! TACGGGTCGGAGTGTTTTTT+TGG + chr1_4:67896872-67896891 Msa0180120:intron 45.0%
TAGTTGTCAAAGCGGAAACG+CGG - chr1_4:67897698-67897717 None:intergenic 45.0%
GGATGGCATAGTTGTCAAAG+CGG - chr1_4:67897706-67897725 None:intergenic 45.0%
ACAAGTGTCATGTACTGGAC+AGG - chr1_4:67898115-67898134 None:intergenic 45.0%
GTCCAGTACATGACACTTGT+AGG + chr1_4:67898115-67898134 Msa0180120:intron 45.0%
CTCCTACAAGTGTCATGTAC+TGG - chr1_4:67898120-67898139 None:intergenic 45.0%
CAGTACATGACACTTGTAGG+AGG + chr1_4:67898118-67898137 Msa0180120:intron 45.0%
TGCACTTGGTTGTTCCGTAA+GGG - chr1_4:67898360-67898379 None:intergenic 45.0%
ATGCACTTGGTTGTTCCGTA+AGG - chr1_4:67898361-67898380 None:intergenic 45.0%
CACACGTGTGCTTACTCAAA+AGG + chr1_4:67898421-67898440 Msa0180120:CDS 45.0%
GACCAAAACTTATGAGAGGG+AGG + chr1_4:67898856-67898875 Msa0180120:three_prime_UTR 45.0%
CCAAAACTTATGAGAGGGAG+GGG + chr1_4:67898858-67898877 Msa0180120:three_prime_UTR 45.0%
!! GGCGTTTTGGTAGATGAATC+CGG + chr1_4:67898879-67898898 Msa0180120:three_prime_UTR 45.0%
!! TAAAAAAATAAATAATTGAA+GGG - chr1_4:67895472-67895491 None:intergenic 5.0%
!! TTAAAAAAATAAATAATTGA+AGG - chr1_4:67895473-67895492 None:intergenic 5.0%
GCAAGCAATACTACTGAGGG+TGG + chr1_4:67895384-67895403 Msa0180120:CDS 50.0%
AGCAATACTACTGAGGGTGG+TGG + chr1_4:67895387-67895406 Msa0180120:CDS 50.0%
TGCCTAAAATTCACGCTGCG+TGG + chr1_4:67895952-67895971 Msa0180120:intron 50.0%
GATTCTCCCCATGGACCAAT+AGG + chr1_4:67896130-67896149 Msa0180120:intron 50.0%
! GGTTGACTTCATGGTTGAGG+TGG + chr1_4:67896635-67896654 Msa0180120:intron 50.0%
ACTTCATGGTTGAGGTGGGA+GGG + chr1_4:67896640-67896659 Msa0180120:intron 50.0%
CGTTTGACAACAGAGGTGGA+TGG - chr1_4:67896711-67896730 None:intergenic 50.0%
TCCACCTCTGTTGTCAAACG+CGG + chr1_4:67896711-67896730 Msa0180120:intron 50.0%
! CTTTTCACAAACGCGCGAAG+CGG - chr1_4:67896798-67896817 None:intergenic 50.0%
CTTCGCGCGTTTGTGAAAAG+CGG + chr1_4:67896798-67896817 Msa0180120:intron 50.0%
TTCGCGCGTTTGTGAAAAGC+GGG + chr1_4:67896799-67896818 Msa0180120:intron 50.0%
AGCTGTGCGGCTATCAAACT+AGG + chr1_4:67897066-67897085 Msa0180120:intron 50.0%
TGGAAACTTATCTGGGCCCT+AGG + chr1_4:67897098-67897117 Msa0180120:intron 50.0%
GGAAACTTATCTGGGCCCTA+GGG + chr1_4:67897099-67897118 Msa0180120:intron 50.0%
GAAACTTATCTGGGCCCTAG+GGG + chr1_4:67897100-67897119 Msa0180120:intron 50.0%
CCTTGTAGATGGACCCCTTA+CGG + chr1_4:67898343-67898362 Msa0180120:intron 50.0%
GCACTTGGTTGTTCCGTAAG+GGG - chr1_4:67898359-67898378 None:intergenic 50.0%
TTGTAAGCAGCAAGTGCACC+AGG + chr1_4:67898384-67898403 Msa0180120:CDS 50.0%
TGTGACAGTACTTGCCATCC+TGG - chr1_4:67898405-67898424 None:intergenic 50.0%
! CCCCTCCCTCTCATAAGTTT+TGG - chr1_4:67898861-67898880 None:intergenic 50.0%
GATGAATCCGGCAAGACGTA+AGG + chr1_4:67898891-67898910 Msa0180120:three_prime_UTR 50.0%
AGAGGAACCTTACGTCTTGC+CGG - chr1_4:67898901-67898920 None:intergenic 50.0%
! GAAGGCTGTCGACGTCGTTT+TGG + chr1_4:67895053-67895072 Msa0180120:five_prime_UTR 55.0%
!!! CTGTCGACGTCGTTTTGGAG+AGG + chr1_4:67895058-67895077 Msa0180120:five_prime_UTR 55.0%
ACAGGGTGAGATTCTCCCCA+TGG + chr1_4:67896121-67896140 Msa0180120:intron 55.0%
GACTTCATGGTTGAGGTGGG+AGG + chr1_4:67896639-67896658 Msa0180120:intron 55.0%
TGGTTGAGGTGGGAGGGTTA+AGG + chr1_4:67896646-67896665 Msa0180120:intron 55.0%
ACCGCGTTTGACAACAGAGG+TGG - chr1_4:67896715-67896734 None:intergenic 55.0%
!! CTGACGCGGTAGGCTGATTT+TGG - chr1_4:67896768-67896787 None:intergenic 55.0%
!! GTTTCGCGTCCGCTTTTACG+TGG + chr1_4:67896821-67896840 Msa0180120:intron 55.0%
!! GTCGATAGCGGGCGCTATTA+CGG + chr1_4:67896854-67896873 Msa0180120:intron 55.0%
!! TCGATAGCGGGCGCTATTAC+GGG + chr1_4:67896855-67896874 Msa0180120:intron 55.0%
GTATTGGGCCTTAGCCCCTA+GGG - chr1_4:67897117-67897136 None:intergenic 55.0%
! AGTATTGGGCCTTAGCCCCT+AGG - chr1_4:67897118-67897137 None:intergenic 55.0%
! TACCGCGCCGCGTCAATTTT+TGG + chr1_4:67897657-67897676 Msa0180120:intron 55.0%
! ACCGCGCCGCGTCAATTTTT+GGG + chr1_4:67897658-67897677 Msa0180120:intron 55.0%
GGCCACCCCAAAAATTGACG+CGG - chr1_4:67897667-67897686 None:intergenic 55.0%
CCGTAAGGGGTCCATCTACA+AGG - chr1_4:67898346-67898365 None:intergenic 55.0%
AAGCAGCAAGTGCACCAGGA+TGG + chr1_4:67898388-67898407 Msa0180120:CDS 55.0%
!!! TATGAGAGGGAGGGGCGTTT+TGG + chr1_4:67898866-67898885 Msa0180120:three_prime_UTR 55.0%
GAGGTGGGAGGGTTAAGGAG+TGG + chr1_4:67896651-67896670 Msa0180120:intron 60.0%
GCGACCGCGTTTGACAACAG+AGG - chr1_4:67896718-67896737 None:intergenic 60.0%
TTGTCAAACGCGGTCGCTCC+CGG + chr1_4:67896721-67896740 Msa0180120:intron 60.0%
TTCACAAACGCGCGAAGCGG+TGG - chr1_4:67896795-67896814 None:intergenic 60.0%
!! GCGTCCGCTTTTACGTGGAG+CGG + chr1_4:67896826-67896845 Msa0180120:intron 60.0%
CGCTCCGCTCCACGTAAAAG+CGG - chr1_4:67896833-67896852 None:intergenic 60.0%
! CGCTTTTACGTGGAGCGGAG+CGG + chr1_4:67896831-67896850 Msa0180120:intron 60.0%
! GCTTTTACGTGGAGCGGAGC+GGG + chr1_4:67896832-67896851 Msa0180120:intron 60.0%
! TAGCGGGCGCTATTACGGGT+CGG + chr1_4:67896859-67896878 Msa0180120:intron 60.0%
TATCTGGGCCCTAGGGGCTA+AGG + chr1_4:67897106-67897125 Msa0180120:intron 60.0%
GCGGGGGCTATTACATGACG+CGG - chr1_4:67897625-67897644 None:intergenic 60.0%
AATTGACGCGGCGCGGTATG+CGG - chr1_4:67897655-67897674 None:intergenic 60.0%
CCCCAAAAATTGACGCGGCG+CGG - chr1_4:67897662-67897681 None:intergenic 60.0%
! CCGCGCCGCGTCAATTTTTG+GGG + chr1_4:67897659-67897678 Msa0180120:intron 60.0%
!! CGCCGCGTCAATTTTTGGGG+TGG + chr1_4:67897662-67897681 Msa0180120:intron 60.0%
GCAGGAGTCTGTGCGATGTG+TGG + chr1_4:67898529-67898548 Msa0180120:intron 60.0%
GAAGCGGTGGCGTTCTGACG+CGG - chr1_4:67896782-67896801 None:intergenic 65.0%
ATTGACGCGGCGCGGTATGC+GGG - chr1_4:67897654-67897673 None:intergenic 65.0%
GTCAAAGCGGAAACGCGGCG+CGG - chr1_4:67897693-67897712 None:intergenic 65.0%
CGGTGGCGTTCTGACGCGGT+AGG - chr1_4:67896778-67896797 None:intergenic 70.0%
GGAGCGGAGCGGGTCGATAG+CGG + chr1_4:67896842-67896861 Msa0180120:intron 70.0%
GAGCGGAGCGGGTCGATAGC+GGG + chr1_4:67896843-67896862 Msa0180120:intron 70.0%
GGTCGCTCCCGGCGCTATCG+CGG + chr1_4:67896732-67896751 Msa0180120:intron 75.0%
GGTATGCGGGCGTGACGCGG+GGG - chr1_4:67897641-67897660 None:intergenic 75.0%
CGGTATGCGGGCGTGACGCG+GGG - chr1_4:67897642-67897661 None:intergenic 75.0%
GCGGTATGCGGGCGTGACGC+GGG - chr1_4:67897643-67897662 None:intergenic 75.0%
CGCGGTATGCGGGCGTGACG+CGG - chr1_4:67897644-67897663 None:intergenic 75.0%
! CTCCCGGCGCTATCGCGGCG+CGG + chr1_4:67896737-67896756 Msa0180120:intron 80.0%
! CTCCGCGCCGCGATAGCGCC+GGG - chr1_4:67896742-67896761 None:intergenic 80.0%
! GCTCCGCGCCGCGATAGCGC+CGG - chr1_4:67896743-67896762 None:intergenic 80.0%
! GGCGCTATCGCGGCGCGGAG+CGG + chr1_4:67896742-67896761 Msa0180120:intron 80.0%
! AGCGGAAACGCGGCGCGGCG+CGG - chr1_4:67897688-67897707 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 67895018 67898957 67895018 ID=Msa0180120;Name=Msa0180120
chr1_4 mRNA 67895018 67898957 67895018 ID=Msa0180120-mRNA-1;Parent=Msa0180120;Name=Msa0180120-mRNA-1;_AED=0.00;_eAED=0.00;_QI=207|1|1|1|1|1|4|360|96
chr1_4 exon 67895018 67895240 67895018 ID=Msa0180120-mRNA-1:exon:22376;Parent=Msa0180120-mRNA-1
chr1_4 exon 67895334 67895451 67895334 ID=Msa0180120-mRNA-1:exon:22377;Parent=Msa0180120-mRNA-1
chr1_4 exon 67898351 67898442 67898351 ID=Msa0180120-mRNA-1:exon:22378;Parent=Msa0180120-mRNA-1
chr1_4 exon 67898533 67898957 67898533 ID=Msa0180120-mRNA-1:exon:22379;Parent=Msa0180120-mRNA-1
chr1_4 five_prime_UTR 67895018 67895224 67895018 ID=Msa0180120-mRNA-1:five_prime_utr;Parent=Msa0180120-mRNA-1
chr1_4 CDS 67895225 67895240 67895225 ID=Msa0180120-mRNA-1:cds;Parent=Msa0180120-mRNA-1
chr1_4 CDS 67895334 67895451 67895334 ID=Msa0180120-mRNA-1:cds;Parent=Msa0180120-mRNA-1
chr1_4 CDS 67898351 67898442 67898351 ID=Msa0180120-mRNA-1:cds;Parent=Msa0180120-mRNA-1
chr1_4 CDS 67898533 67898597 67898533 ID=Msa0180120-mRNA-1:cds;Parent=Msa0180120-mRNA-1
chr1_4 three_prime_UTR 67898598 67898957 67898598 ID=Msa0180120-mRNA-1:three_prime_utr;Parent=Msa0180120-mRNA-1
Gene Sequence

>Msa0180120

ATGGTCTGCGAAAAGTGTCAGAAGAAATTATCGAAGGTGATAGTACCAGATAAATGGAAAGAAGGTGCAAGCAATACTACTGAGGGTGGTGGCCGCAAAATCAACGAGAACAAACTCCTTTCCAAGAAGAACAGATGGACCCCTTACGGAACAACCAAGTGCATCATTTGTAAGCAGCAAGTGCACCAGGATGGCAAGTACTGTCACACGTGTGCTTACTCAAAAGGAGTCTGTGCGATGTGTGGCAAGCAAGTTCTTGACACCAAGTTTTATAAACAAAGCAACGTGTAG
Protein sequence

>Msa0180120

MVCEKCQKKLSKVIVPDKWKEGASNTTEGGGRKINENKLLSKKNRWTPYGTTKCIICKQQVHQDGKYCHTCAYSKGVCAMCGKQVLDTKFYKQSNV*