AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0460100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0460100 MtrunA17_Chr3g0109351 100.000 71 0 0 1 71 1 71 3.61e-47 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0460100 AT3G14430.1 63.492 63 22 1 9 71 15 76 3.19e-24 86.7

Find 39 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGACTGATTGTTAGAATT+TGG 0.141769 3_3:-57127304 Msa0460100:five_prime_UTR
TCATACTCAGTTCTCAAATT+TGG 0.144615 3_3:-57125839 Msa0460100:three_prime_UTR
CAAACATCAATTCCTTTAAA+AGG 0.150864 3_3:+57126189 None:intergenic
TATCTTTCATGTCAAAGATA+TGG 0.312816 3_3:+57125791 None:intergenic
ATTTCTCACAAAGGCGAAAA+TGG 0.321389 3_3:+57127205 None:intergenic
GTTAATGTTACATTCTAGTT+AGG 0.334854 3_3:-57125993 Msa0460100:three_prime_UTR
ACAAATACTTGATTGTATTC+AGG 0.336136 3_3:+57126244 None:intergenic
GTATCTTTATGTAAGTTTGT+AGG 0.358607 3_3:-57126050 Msa0460100:three_prime_UTR
TTTGAGAACTGAGTATGAAT+AGG 0.363509 3_3:+57125844 None:intergenic
CTGGTGGTGGACCAGAGCTT+TGG 0.389186 3_3:-57126213 Msa0460100:CDS
CTTTAAAAGGGCCAAAGCTC+TGG 0.402112 3_3:+57126202 None:intergenic
TCTCATTCTATATCTCGTTT+CGG 0.403988 3_3:-57125895 Msa0460100:three_prime_UTR
ATCTCGTTTCGGTTTAGCTT+AGG 0.404307 3_3:-57125884 Msa0460100:three_prime_UTR
TGTCTGTCTCTATTTCAGAT+TGG 0.429046 3_3:-57125927 Msa0460100:three_prime_UTR
AAACATCAATTCCTTTAAAA+GGG 0.443719 3_3:+57126190 None:intergenic
AGCTTAGGAACAGAAATGCT+TGG 0.457112 3_3:-57125869 Msa0460100:three_prime_UTR
TTGAGAACTGAGTATGAATA+GGG 0.461008 3_3:+57125845 None:intergenic
AAAGGAATTGATGTTTGGAC+AGG 0.475928 3_3:-57126183 Msa0460100:three_prime_UTR
CAAAGCTCTGGTCCACCACC+AGG 0.488950 3_3:+57126214 None:intergenic
CAAGTATTTGTGAAGAAGCC+TGG 0.508253 3_3:-57126232 Msa0460100:CDS
GACAGGTTGGACGTCATCCA+TGG 0.522357 3_3:-57126166 Msa0460100:three_prime_UTR
CATAAAGATACAAATACAAA+CGG 0.528427 3_3:+57126062 None:intergenic
GAATTGATGTTTGGACAGGT+TGG 0.543485 3_3:-57126179 Msa0460100:three_prime_UTR
TAAAACAGAGAGAAGAAGAC+TGG 0.550409 3_3:-57127323 Msa0460100:five_prime_UTR
GACTGATTGTTAGAATTTGG+AGG 0.551707 3_3:-57127301 Msa0460100:five_prime_UTR
CAGGCACGTACAAATACAAA+CGG 0.558174 3_3:+57126103 None:intergenic
TGAAGAAGAAAAGAAAGAAG+AGG 0.565354 3_3:+57127168 None:intergenic
GAGAGAAAACAACGATGGCT+AGG 0.568009 3_3:-57127277 Msa0460100:exon
GAAATATGTAAAATCATCAC+AGG 0.585504 3_3:+57126084 None:intergenic
GAAATATGCGAAATCATCAC+AGG 0.596731 3_3:+57126125 None:intergenic
TTTGTGAAGAAGCCTGGTGG+TGG 0.604245 3_3:-57126226 Msa0460100:CDS
AGAACGAGTCGTTATTGACT+CGG 0.608689 3_3:-57127238 Msa0460100:CDS
ATTAACATCATTCAAATCTG+TGG 0.614330 3_3:+57126010 None:intergenic
AAACTCAGCATTTCTCACAA+AGG 0.666973 3_3:+57127196 None:intergenic
AGAAAACAACGATGGCTAGG+CGG 0.694012 3_3:-57127274 Msa0460100:exon
GCGTGCTTAGAATACCTCCA+TGG 0.702615 3_3:+57126149 None:intergenic
GAGGTGAGAGAAAACAACGA+TGG 0.733406 3_3:-57127282 Msa0460100:exon
AGGTTGGACGTCATCCATGG+AGG 0.746774 3_3:-57126163 Msa0460100:three_prime_UTR
GTATTTGTGAAGAAGCCTGG+TGG 0.787083 3_3:-57126229 Msa0460100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGGATAATTATATCAAAA+AGG - chr3_3:57126141-57126160 Msa0460100:three_prime_UTR 15.0%
!! ATTGGATAATTATATCAAAA+AGG - chr3_3:57126141-57126160 Msa0460100:three_prime_UTR 15.0%
!!! CTTTTAATGTTTGAGAAATT+AGG - chr3_3:57126057-57126076 Msa0460100:three_prime_UTR 20.0%
!! TTAGTTACTAAATTGAAAAC+TGG - chr3_3:57126107-57126126 Msa0460100:three_prime_UTR 20.0%
!! AGATTCACAAATTTATATGT+CGG - chr3_3:57126377-57126396 Msa0460100:intron 20.0%
!! CTTCATAATTCATTCATATA+TGG - chr3_3:57126661-57126680 Msa0460100:intron 20.0%
!! ATAATTCATTCATATATGGT+TGG - chr3_3:57126665-57126684 Msa0460100:intron 20.0%
!!! TTTTGATAAGTGGAAATTTT+TGG - chr3_3:57126688-57126707 Msa0460100:intron 20.0%
!!! AAACATCAATTCCTTTAAAA+GGG + chr3_3:57126888-57126907 None:intergenic 20.0%
!! CATAAAGATACAAATACAAA+CGG + chr3_3:57127016-57127035 None:intergenic 20.0%
!!! CTTTTAATGTTTGAGAAATT+AGG - chr3_3:57126057-57126076 Msa0460100:three_prime_UTR 20.0%
!! TTAGTTACTAAATTGAAAAC+TGG - chr3_3:57126107-57126126 Msa0460100:three_prime_UTR 20.0%
!! AGATTCACAAATTTATATGT+CGG - chr3_3:57126377-57126396 Msa0460100:intron 20.0%
!! CTTCATAATTCATTCATATA+TGG - chr3_3:57126661-57126680 Msa0460100:intron 20.0%
!! ATAATTCATTCATATATGGT+TGG - chr3_3:57126665-57126684 Msa0460100:intron 20.0%
!!! TTTTGATAAGTGGAAATTTT+TGG - chr3_3:57126688-57126707 Msa0460100:intron 20.0%
!!! AAACATCAATTCCTTTAAAA+GGG + chr3_3:57126888-57126907 None:intergenic 20.0%
!! CATAAAGATACAAATACAAA+CGG + chr3_3:57127016-57127035 None:intergenic 20.0%
!!! AATTTTCGTTCTTTTTCATC+TGG - chr3_3:57126021-57126040 Msa0460100:three_prime_UTR 25.0%
!!! ATTTTCGTTCTTTTTCATCT+GGG - chr3_3:57126022-57126041 Msa0460100:three_prime_UTR 25.0%
!!! ATTTTGATTCCAATTGGAAT+TGG - chr3_3:57126169-57126188 Msa0460100:three_prime_UTR 25.0%
! AACTATATACCAATTCCAAT+TGG + chr3_3:57126181-57126200 None:intergenic 25.0%
! TTAAATTACAATCAGCATGT+TGG - chr3_3:57126444-57126463 Msa0460100:intron 25.0%
! TTCAATGTAATCATATGTGT+CGG - chr3_3:57126569-57126588 Msa0460100:intron 25.0%
! TATGAATGAATTATGAAGCA+CGG + chr3_3:57126659-57126678 None:intergenic 25.0%
! ACAAATACTTGATTGTATTC+AGG + chr3_3:57126834-57126853 None:intergenic 25.0%
! CAAACATCAATTCCTTTAAA+AGG + chr3_3:57126889-57126908 None:intergenic 25.0%
!!! CTTTTAAAGGAATTGATGTT+TGG - chr3_3:57126887-57126906 Msa0460100:intron 25.0%
! GAAATATGTAAAATCATCAC+AGG + chr3_3:57126994-57127013 None:intergenic 25.0%
! GTATCTTTATGTAAGTTTGT+AGG - chr3_3:57127025-57127044 Msa0460100:intron 25.0%
! ATTAACATCATTCAAATCTG+TGG + chr3_3:57127068-57127087 None:intergenic 25.0%
!! GTTAATGTTACATTCTAGTT+AGG - chr3_3:57127082-57127101 Msa0460100:intron 25.0%
! TATCTTTCATGTCAAAGATA+TGG + chr3_3:57127287-57127306 None:intergenic 25.0%
!!! AATTTTCGTTCTTTTTCATC+TGG - chr3_3:57126021-57126040 Msa0460100:three_prime_UTR 25.0%
!!! ATTTTCGTTCTTTTTCATCT+GGG - chr3_3:57126022-57126041 Msa0460100:three_prime_UTR 25.0%
!!! ATTTTGATTCCAATTGGAAT+TGG - chr3_3:57126169-57126188 Msa0460100:three_prime_UTR 25.0%
! AACTATATACCAATTCCAAT+TGG + chr3_3:57126181-57126200 None:intergenic 25.0%
! TTAAATTACAATCAGCATGT+TGG - chr3_3:57126444-57126463 Msa0460100:intron 25.0%
! TTCAATGTAATCATATGTGT+CGG - chr3_3:57126569-57126588 Msa0460100:intron 25.0%
! TATGAATGAATTATGAAGCA+CGG + chr3_3:57126659-57126678 None:intergenic 25.0%
! ACAAATACTTGATTGTATTC+AGG + chr3_3:57126834-57126853 None:intergenic 25.0%
! CAAACATCAATTCCTTTAAA+AGG + chr3_3:57126889-57126908 None:intergenic 25.0%
!!! CTTTTAAAGGAATTGATGTT+TGG - chr3_3:57126887-57126906 Msa0460100:intron 25.0%
! GAAATATGTAAAATCATCAC+AGG + chr3_3:57126994-57127013 None:intergenic 25.0%
! GTATCTTTATGTAAGTTTGT+AGG - chr3_3:57127025-57127044 Msa0460100:intron 25.0%
! ATTAACATCATTCAAATCTG+TGG + chr3_3:57127068-57127087 None:intergenic 25.0%
!! GTTAATGTTACATTCTAGTT+AGG - chr3_3:57127082-57127101 Msa0460100:intron 25.0%
! TATCTTTCATGTCAAAGATA+TGG + chr3_3:57127287-57127306 None:intergenic 25.0%
TGAAGAAGAAAAGAAAGAAG+AGG + chr3_3:57125910-57125929 None:intergenic 30.0%
ATTTGAACAAATGATGCAAC+TGG + chr3_3:57126004-57126023 None:intergenic 30.0%
!! AAACTGGTTTGATGATTGAT+TGG - chr3_3:57126123-57126142 Msa0460100:three_prime_UTR 30.0%
!!! GTAGAGATTTTGATTCCAAT+TGG - chr3_3:57126163-57126182 Msa0460100:three_prime_UTR 30.0%
!!! ATATGGTTGGTTTTGATAAG+TGG - chr3_3:57126678-57126697 Msa0460100:intron 30.0%
! TTGTCTTGCAATTTCACTTT+TGG - chr3_3:57126763-57126782 Msa0460100:intron 30.0%
TCTCATTCTATATCTCGTTT+CGG - chr3_3:57127180-57127199 Msa0460100:CDS 30.0%
TTGAGAACTGAGTATGAATA+GGG + chr3_3:57127233-57127252 None:intergenic 30.0%
TTTGAGAACTGAGTATGAAT+AGG + chr3_3:57127234-57127253 None:intergenic 30.0%
TCATACTCAGTTCTCAAATT+TGG - chr3_3:57127236-57127255 Msa0460100:CDS 30.0%
TGAAGAAGAAAAGAAAGAAG+AGG + chr3_3:57125910-57125929 None:intergenic 30.0%
ATTTGAACAAATGATGCAAC+TGG + chr3_3:57126004-57126023 None:intergenic 30.0%
!! AAACTGGTTTGATGATTGAT+TGG - chr3_3:57126123-57126142 Msa0460100:three_prime_UTR 30.0%
!!! GTAGAGATTTTGATTCCAAT+TGG - chr3_3:57126163-57126182 Msa0460100:three_prime_UTR 30.0%
!!! ATATGGTTGGTTTTGATAAG+TGG - chr3_3:57126678-57126697 Msa0460100:intron 30.0%
! TTGTCTTGCAATTTCACTTT+TGG - chr3_3:57126763-57126782 Msa0460100:intron 30.0%
TCTCATTCTATATCTCGTTT+CGG - chr3_3:57127180-57127199 Msa0460100:CDS 30.0%
TTGAGAACTGAGTATGAATA+GGG + chr3_3:57127233-57127252 None:intergenic 30.0%
TTTGAGAACTGAGTATGAAT+AGG + chr3_3:57127234-57127253 None:intergenic 30.0%
TCATACTCAGTTCTCAAATT+TGG - chr3_3:57127236-57127255 Msa0460100:CDS 30.0%
TAAAACAGAGAGAAGAAGAC+TGG - chr3_3:57125752-57125771 Msa0460100:three_prime_UTR 35.0%
CTGGACTGATTGTTAGAATT+TGG - chr3_3:57125771-57125790 Msa0460100:three_prime_UTR 35.0%
GACTGATTGTTAGAATTTGG+AGG - chr3_3:57125774-57125793 Msa0460100:three_prime_UTR 35.0%
ATTTCTCACAAAGGCGAAAA+TGG + chr3_3:57125873-57125892 None:intergenic 35.0%
AAACTCAGCATTTCTCACAA+AGG + chr3_3:57125882-57125901 None:intergenic 35.0%
CATAAAGAGAAAAAACAGCG+AGG + chr3_3:57125970-57125989 None:intergenic 35.0%
GATTACGAGTCTGTTTGAAT+TGG + chr3_3:57126421-57126440 None:intergenic 35.0%
TGAAATTGCAAGACAAACAG+AGG + chr3_3:57126759-57126778 None:intergenic 35.0%
AAAGGAATTGATGTTTGGAC+AGG - chr3_3:57126892-57126911 Msa0460100:intron 35.0%
GAAATATGCGAAATCATCAC+AGG + chr3_3:57126953-57126972 None:intergenic 35.0%
TGTCTGTCTCTATTTCAGAT+TGG - chr3_3:57127148-57127167 Msa0460100:CDS 35.0%
TAAAACAGAGAGAAGAAGAC+TGG - chr3_3:57125752-57125771 Msa0460100:three_prime_UTR 35.0%
CTGGACTGATTGTTAGAATT+TGG - chr3_3:57125771-57125790 Msa0460100:three_prime_UTR 35.0%
GACTGATTGTTAGAATTTGG+AGG - chr3_3:57125774-57125793 Msa0460100:three_prime_UTR 35.0%
ATTTCTCACAAAGGCGAAAA+TGG + chr3_3:57125873-57125892 None:intergenic 35.0%
AAACTCAGCATTTCTCACAA+AGG + chr3_3:57125882-57125901 None:intergenic 35.0%
CATAAAGAGAAAAAACAGCG+AGG + chr3_3:57125970-57125989 None:intergenic 35.0%
GATTACGAGTCTGTTTGAAT+TGG + chr3_3:57126421-57126440 None:intergenic 35.0%
TGAAATTGCAAGACAAACAG+AGG + chr3_3:57126759-57126778 None:intergenic 35.0%
AAAGGAATTGATGTTTGGAC+AGG - chr3_3:57126892-57126911 Msa0460100:intron 35.0%
GAAATATGCGAAATCATCAC+AGG + chr3_3:57126953-57126972 None:intergenic 35.0%
TGTCTGTCTCTATTTCAGAT+TGG - chr3_3:57127148-57127167 Msa0460100:CDS 35.0%
! AGAACGAGTCGTTATTGACT+CGG - chr3_3:57125837-57125856 Msa0460100:three_prime_UTR 40.0%
ACTGATGAAAACAACTTGCG+AGG + chr3_3:57126347-57126366 None:intergenic 40.0%
!!! ACAATCAGCATGTTGGCTTT+TGG - chr3_3:57126451-57126470 Msa0460100:intron 40.0%
TGGTGTGTCATGTATGAACA+CGG - chr3_3:57126471-57126490 Msa0460100:intron 40.0%
ATTGCAAGACAAACAGAGGA+GGG + chr3_3:57126755-57126774 None:intergenic 40.0%
AATTGCAAGACAAACAGAGG+AGG + chr3_3:57126756-57126775 None:intergenic 40.0%
CAAGTATTTGTGAAGAAGCC+TGG - chr3_3:57126843-57126862 Msa0460100:intron 40.0%
GAATTGATGTTTGGACAGGT+TGG - chr3_3:57126896-57126915 Msa0460100:intron 40.0%
CAGGCACGTACAAATACAAA+CGG + chr3_3:57126975-57126994 None:intergenic 40.0%
! ATCTCGTTTCGGTTTAGCTT+AGG - chr3_3:57127191-57127210 Msa0460100:CDS 40.0%
AGCTTAGGAACAGAAATGCT+TGG - chr3_3:57127206-57127225 Msa0460100:CDS 40.0%
! AGAACGAGTCGTTATTGACT+CGG - chr3_3:57125837-57125856 Msa0460100:three_prime_UTR 40.0%
ACTGATGAAAACAACTTGCG+AGG + chr3_3:57126347-57126366 None:intergenic 40.0%
!!! ACAATCAGCATGTTGGCTTT+TGG - chr3_3:57126451-57126470 Msa0460100:intron 40.0%
TGGTGTGTCATGTATGAACA+CGG - chr3_3:57126471-57126490 Msa0460100:intron 40.0%
ATTGCAAGACAAACAGAGGA+GGG + chr3_3:57126755-57126774 None:intergenic 40.0%
AATTGCAAGACAAACAGAGG+AGG + chr3_3:57126756-57126775 None:intergenic 40.0%
CAAGTATTTGTGAAGAAGCC+TGG - chr3_3:57126843-57126862 Msa0460100:intron 40.0%
GAATTGATGTTTGGACAGGT+TGG - chr3_3:57126896-57126915 Msa0460100:intron 40.0%
CAGGCACGTACAAATACAAA+CGG + chr3_3:57126975-57126994 None:intergenic 40.0%
! ATCTCGTTTCGGTTTAGCTT+AGG - chr3_3:57127191-57127210 Msa0460100:CDS 40.0%
AGCTTAGGAACAGAAATGCT+TGG - chr3_3:57127206-57127225 Msa0460100:CDS 40.0%
GAGGTGAGAGAAAACAACGA+TGG - chr3_3:57125793-57125812 Msa0460100:three_prime_UTR 45.0%
GAGAGAAAACAACGATGGCT+AGG - chr3_3:57125798-57125817 Msa0460100:three_prime_UTR 45.0%
AGAAAACAACGATGGCTAGG+CGG - chr3_3:57125801-57125820 Msa0460100:three_prime_UTR 45.0%
TGCAGACATCAGACACGAAA+CGG - chr3_3:57126501-57126520 Msa0460100:intron 45.0%
TTCTCAGATTAGGTGTGTCC+TGG + chr3_3:57126632-57126651 None:intergenic 45.0%
GTATTTGTGAAGAAGCCTGG+TGG - chr3_3:57126846-57126865 Msa0460100:intron 45.0%
! CTTTAAAAGGGCCAAAGCTC+TGG + chr3_3:57126876-57126895 None:intergenic 45.0%
!! CAGAGCTTTGGCCCTTTTAA+AGG - chr3_3:57126874-57126893 Msa0460100:intron 45.0%
GAGGTGAGAGAAAACAACGA+TGG - chr3_3:57125793-57125812 Msa0460100:three_prime_UTR 45.0%
GAGAGAAAACAACGATGGCT+AGG - chr3_3:57125798-57125817 Msa0460100:three_prime_UTR 45.0%
AGAAAACAACGATGGCTAGG+CGG - chr3_3:57125801-57125820 Msa0460100:three_prime_UTR 45.0%
TGCAGACATCAGACACGAAA+CGG - chr3_3:57126501-57126520 Msa0460100:intron 45.0%
TTCTCAGATTAGGTGTGTCC+TGG + chr3_3:57126632-57126651 None:intergenic 45.0%
GTATTTGTGAAGAAGCCTGG+TGG - chr3_3:57126846-57126865 Msa0460100:intron 45.0%
! CTTTAAAAGGGCCAAAGCTC+TGG + chr3_3:57126876-57126895 None:intergenic 45.0%
!! CAGAGCTTTGGCCCTTTTAA+AGG - chr3_3:57126874-57126893 Msa0460100:intron 45.0%
!!! TTATTTTATCAAATTATTAT+TGG + chr3_3:57126544-57126563 None:intergenic 5.0%
!!! TTATTTTATCAAATTATTAT+TGG + chr3_3:57126544-57126563 None:intergenic 5.0%
GTCATGTATGAACACGGACG+CGG - chr3_3:57126477-57126496 Msa0460100:intron 50.0%
CATCAGACACGAAACGGACA+TGG - chr3_3:57126507-57126526 Msa0460100:intron 50.0%
!! CATATGTGTCGGTGTCGTGT+TGG - chr3_3:57126580-57126599 Msa0460100:intron 50.0%
GATTAGGTGTGTCCTGGTGT+CGG + chr3_3:57126626-57126645 None:intergenic 50.0%
GCACGGACACTTCTCAGATT+AGG + chr3_3:57126642-57126661 None:intergenic 50.0%
AGACAAACAGAGGAGGGAGT+GGG + chr3_3:57126749-57126768 None:intergenic 50.0%
AAGACAAACAGAGGAGGGAG+TGG + chr3_3:57126750-57126769 None:intergenic 50.0%
TTTGTGAAGAAGCCTGGTGG+TGG - chr3_3:57126849-57126868 Msa0460100:intron 50.0%
GCGTGCTTAGAATACCTCCA+TGG + chr3_3:57126929-57126948 None:intergenic 50.0%
GTCATGTATGAACACGGACG+CGG - chr3_3:57126477-57126496 Msa0460100:intron 50.0%
CATCAGACACGAAACGGACA+TGG - chr3_3:57126507-57126526 Msa0460100:intron 50.0%
!! CATATGTGTCGGTGTCGTGT+TGG - chr3_3:57126580-57126599 Msa0460100:intron 50.0%
GATTAGGTGTGTCCTGGTGT+CGG + chr3_3:57126626-57126645 None:intergenic 50.0%
GCACGGACACTTCTCAGATT+AGG + chr3_3:57126642-57126661 None:intergenic 50.0%
AGACAAACAGAGGAGGGAGT+GGG + chr3_3:57126749-57126768 None:intergenic 50.0%
AAGACAAACAGAGGAGGGAG+TGG + chr3_3:57126750-57126769 None:intergenic 50.0%
TTTGTGAAGAAGCCTGGTGG+TGG - chr3_3:57126849-57126868 Msa0460100:intron 50.0%
GCGTGCTTAGAATACCTCCA+TGG + chr3_3:57126929-57126948 None:intergenic 50.0%
! TGTCGGACACGCATTGGTGT+CGG + chr3_3:57126609-57126628 None:intergenic 55.0%
ACAAACAGAGGAGGGAGTGG+GGG + chr3_3:57126747-57126766 None:intergenic 55.0%
GACAAACAGAGGAGGGAGTG+GGG + chr3_3:57126748-57126767 None:intergenic 55.0%
! GACAGGTTGGACGTCATCCA+TGG - chr3_3:57126909-57126928 Msa0460100:intron 55.0%
AGGTTGGACGTCATCCATGG+AGG - chr3_3:57126912-57126931 Msa0460100:intron 55.0%
! TGTCGGACACGCATTGGTGT+CGG + chr3_3:57126609-57126628 None:intergenic 55.0%
ACAAACAGAGGAGGGAGTGG+GGG + chr3_3:57126747-57126766 None:intergenic 55.0%
GACAAACAGAGGAGGGAGTG+GGG + chr3_3:57126748-57126767 None:intergenic 55.0%
! GACAGGTTGGACGTCATCCA+TGG - chr3_3:57126909-57126928 Msa0460100:intron 55.0%
AGGTTGGACGTCATCCATGG+AGG - chr3_3:57126912-57126931 Msa0460100:intron 55.0%
ACCAATGCGTGTCCGACACC+AGG - chr3_3:57126611-57126630 Msa0460100:intron 60.0%
! TCCTGGTGTCGGACACGCAT+TGG + chr3_3:57126615-57126634 None:intergenic 60.0%
! CAAAGCTCTGGTCCACCACC+AGG + chr3_3:57126864-57126883 None:intergenic 60.0%
!! CTGGTGGTGGACCAGAGCTT+TGG - chr3_3:57126862-57126881 Msa0460100:intron 60.0%
ACCAATGCGTGTCCGACACC+AGG - chr3_3:57126611-57126630 Msa0460100:intron 60.0%
! TCCTGGTGTCGGACACGCAT+TGG + chr3_3:57126615-57126634 None:intergenic 60.0%
! CAAAGCTCTGGTCCACCACC+AGG + chr3_3:57126864-57126883 None:intergenic 60.0%
!! CTGGTGGTGGACCAGAGCTT+TGG - chr3_3:57126862-57126881 Msa0460100:intron 60.0%
AACAGAGGAGGGAGTGGGGG+TGG + chr3_3:57126744-57126763 None:intergenic 65.0%
AACAGAGGAGGGAGTGGGGG+TGG + chr3_3:57126744-57126763 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 57125752 57127345 57125752 ID=Msa0460100;Name=Msa0460100
chr3_3 mRNA 57125752 57127345 57125752 ID=Msa0460100-mRNA-1;Parent=Msa0460100;Name=Msa0460100-mRNA-1;_AED=0.02;_eAED=0.02;_QI=60|1|1|1|1|1|2|452|71
chr3_3 exon 57125752 57126275 57125752 ID=Msa0460100-mRNA-1:exon:11605;Parent=Msa0460100-mRNA-1
chr3_3 exon 57127142 57127345 57127142 ID=Msa0460100-mRNA-1:exon:11604;Parent=Msa0460100-mRNA-1
chr3_3 five_prime_UTR 57127286 57127345 57127286 ID=Msa0460100-mRNA-1:five_prime_utr;Parent=Msa0460100-mRNA-1
chr3_3 CDS 57127142 57127285 57127142 ID=Msa0460100-mRNA-1:cds;Parent=Msa0460100-mRNA-1
chr3_3 CDS 57126204 57126275 57126204 ID=Msa0460100-mRNA-1:cds;Parent=Msa0460100-mRNA-1
chr3_3 three_prime_UTR 57125752 57126203 57125752 ID=Msa0460100-mRNA-1:three_prime_utr;Parent=Msa0460100-mRNA-1
Gene Sequence

>Msa0460100

ATGGCTAGGCGGCGAGTCGAGACTCAGAACGAGTCGTTATTGACTCGGATTGTTGATTCCATTTTCGCCTTTGTGAGAAATGCTGAGTTTGAGATCCTCTTCTTTCTTTTCTTCTTCATCGCTTATATTCTGTTCAAAGACATCACATCAAGACCTGAATACAATCAAGTATTTGTGAAGAAGCCTGGTGGTGGACCAGAGCTTTGGCCCTTTTAA
Protein sequence

>Msa0460100

MARRRVETQNESLLTRIVDSIFAFVRNAEFEILFFLFFFIAYILFKDITSRPEYNQVFVKKPGGGPELWPF*