AlfalfaGEDB | Msa1255850

M. sativa cultivar zhongmu-4 / Msa1255850

KEGG: K05864
GO: -
PFAM: PF00160.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255850	XP_003629580.1	99.444	360	2	0	1	360	1	360	0.0	739

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255850	sp\|Q9C566\|CYP40_ARATH	71.111	360	103	1	1	359	1	360	0.0	545

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255850	G7LGD2	99.444	360	2	0	1	360	1	360	0.0	739

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001760	Msa1255850	0.804791	1.823436e-49	-8.615850e-47
Msa0021290	Msa1255850	0.826410	2.754129e-54	-8.615850e-47
Msa0021320	Msa1255850	0.836092	1.145565e-56	-8.615850e-47
Msa0040220	Msa1255850	0.877868	4.449891e-69	-8.615850e-47
Msa0080370	Msa1255850	0.815929	7.187127e-52	-8.615850e-47
Msa0086160	Msa1255850	0.830999	2.139459e-55	-8.615850e-47
Msa0087910	Msa1255850	0.882243	1.226422e-70	-8.615850e-47
Msa0100600	Msa1255850	0.819250	1.281729e-52	-8.615850e-47
Msa0100890	Msa1255850	0.814110	1.820574e-51	-8.615850e-47
Msa0114600	Msa1255850	0.843255	1.567690e-58	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255850	MtrunA17_Chr8g0374621	99.444	360	2	0	1	360	1	360	0.0	739
Msa1255850	MtrunA17_Chr4g0045321	73.961	361	93	1	1	360	1	361	0.0	565
Msa1255850	MtrunA17_Chr7g0274841	63.429	175	60	3	1	174	1	172	8.25e-71	218
Msa1255850	MtrunA17_Chr7g0260441	60.773	181	68	2	3	182	6	184	2.07e-69	229
Msa1255850	MtrunA17_Chr4g0038431	61.143	175	64	3	1	174	1	172	2.11e-69	215
Msa1255850	MtrunA17_Chr3g0104751	60.355	169	65	2	5	173	35	201	3.33e-64	202
Msa1255850	MtrunA17_Chr3g0113441	58.480	171	69	2	5	175	65	233	1.32e-63	202
Msa1255850	MtrunA17_Chr3g0145501	58.140	172	68	3	5	175	13	181	2.34e-63	200
Msa1255850	MtrunA17_Chr1g0213971	57.558	172	62	2	5	173	78	241	3.69e-61	196
Msa1255850	MtrunA17_Chr7g0270991	55.030	169	68	1	5	173	97	257	1.14e-59	193

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255850	AT2G15790.1	71.111	360	103	1	1	359	1	360	0.0	545
Msa1255850	AT3G63400.2	66.667	174	55	2	3	175	6	177	5.70e-75	237
Msa1255850	AT3G63400.3	66.667	174	55	2	3	175	6	177	5.36e-73	237
Msa1255850	AT3G63400.4	66.667	174	55	2	3	175	6	177	5.36e-73	237
Msa1255850	AT3G63400.1	66.667	174	55	2	3	175	6	177	5.36e-73	237
Msa1255850	AT4G34870.1	62.857	175	61	3	1	174	1	172	6.01e-71	219
Msa1255850	AT2G16600.1	63.006	173	60	3	3	174	4	173	8.69e-71	219
Msa1255850	AT4G38740.1	61.714	175	63	3	1	174	1	172	3.96e-70	217
Msa1255850	AT2G21130.1	61.494	174	63	3	3	175	4	174	6.39e-70	217
Msa1255850	AT3G56070.1	63.218	174	60	3	1	173	1	171	1.24e-69	216

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Find 117 sgRNAs with CRISPR-Local

Find 302 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGCTTCATGATCCAAGG+CGG	0.764461	8_2:+55155131	Msa1255850:CDS
AACTGACTCCCATTGGTGTT+TGG	0.342691	8_2:-55155272	None:intergenic
AAGCATCGGCGGTGAACTCG+AGG	0.527582	8_2:+55154965	Msa1255850:CDS
AATACCGGTCCAAACACCAA+TGG	0.627525	8_2:+55155263	Msa1255850:CDS
AATAGTCACCAACCATCATT+TGG	0.395698	8_2:-55157759	None:intergenic
AATCAAGTAAAGCTCCACTG+AGG	0.768633	8_2:-55156949	None:intergenic
AATGAAGATGAAGAGTGAAG+TGG	0.522437	8_2:-55154881	None:intergenic
AATGCTGTTGAATCTATCAA+GGG	0.636468	8_2:+55155563	Msa1255850:CDS
AATTAGCCTTGATGCGGATA+TGG	0.331853	8_2:+55158573	Msa1255850:exon
AATTTCCGATCTCTCTGCAC+CGG	0.632806	8_2:+55155038	Msa1255850:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGCTGTTGAATCTATCAA+GGG	+	chr8_2:55155563-55155582	Msa1255850:CDS	30.0%
CCAAATAAAAGATACACACT+AGG	-	chr8_2:55155719-55155738	None:intergenic	30.0%
TAATCAGCAGATCATAAGAA+TGG	-	chr8_2:55155893-55155912	None:intergenic	30.0%
ACAGAAGCAAGATTATAAGA+TGG	+	chr8_2:55155948-55155967	Msa1255850:intron	30.0%
AGAAAAACAGTAAGTAACCA+CGG	-	chr8_2:55156099-55156118	None:intergenic	30.0%
TCAAATCTTTACCAACAGTT+CGG	+	chr8_2:55156194-55156213	Msa1255850:CDS	30.0%
TAAAATAGTCATTCACAAGG+TGG	-	chr8_2:55156637-55156656	None:intergenic	30.0%
AGAATGTGACACATTATAAC+AGG	-	chr8_2:55156847-55156866	None:intergenic	30.0%
CAGGCCTGTAAATTAAAATT+AGG	+	chr8_2:55156923-55156942	Msa1255850:intron	30.0%
AGAGTTTCAATAGAGAAAGT+AGG	+	chr8_2:55157044-55157063	Msa1255850:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	55154883	55158910	55154883	ID=Msa1255850;Name=Msa1255850
chr8_2	mRNA	55154883	55158910	55154883	ID=Msa1255850-mRNA-1;Parent=Msa1255850;Name=Msa1255850-mRNA-1;_AED=0.06;_eAED=0.06;_QI=53\|1\|1\|1\|1\|1\|7\|332\|360
chr8_2	exon	55154883	55155607	55154883	ID=Msa1255850-mRNA-1:exon:17013;Parent=Msa1255850-mRNA-1
chr8_2	exon	55155952	55156039	55155952	ID=Msa1255850-mRNA-1:exon:17014;Parent=Msa1255850-mRNA-1
chr8_2	exon	55156163	55156215	55156163	ID=Msa1255850-mRNA-1:exon:17015;Parent=Msa1255850-mRNA-1
chr8_2	exon	55156926	55157027	55156926	ID=Msa1255850-mRNA-1:exon:17016;Parent=Msa1255850-mRNA-1
chr8_2	exon	55157693	55157768	55157693	ID=Msa1255850-mRNA-1:exon:17017;Parent=Msa1255850-mRNA-1
chr8_2	exon	55158391	55158428	55158391	ID=Msa1255850-mRNA-1:exon:17018;Parent=Msa1255850-mRNA-1
chr8_2	exon	55158525	55158910	55158525	ID=Msa1255850-mRNA-1:exon:17019;Parent=Msa1255850-mRNA-1
chr8_2	five_prime_UTR	55154883	55154935	55154883	ID=Msa1255850-mRNA-1:five_prime_utr;Parent=Msa1255850-mRNA-1
chr8_2	CDS	55154936	55155607	55154936	ID=Msa1255850-mRNA-1:cds;Parent=Msa1255850-mRNA-1
chr8_2	CDS	55155952	55156039	55155952	ID=Msa1255850-mRNA-1:cds;Parent=Msa1255850-mRNA-1
chr8_2	CDS	55156163	55156215	55156163	ID=Msa1255850-mRNA-1:cds;Parent=Msa1255850-mRNA-1
chr8_2	CDS	55156926	55157027	55156926	ID=Msa1255850-mRNA-1:cds;Parent=Msa1255850-mRNA-1
chr8_2	CDS	55157693	55157768	55157693	ID=Msa1255850-mRNA-1:cds;Parent=Msa1255850-mRNA-1
chr8_2	CDS	55158391	55158428	55158391	ID=Msa1255850-mRNA-1:cds;Parent=Msa1255850-mRNA-1
chr8_2	CDS	55158525	55158578	55158525	ID=Msa1255850-mRNA-1:cds;Parent=Msa1255850-mRNA-1
chr8_2	three_prime_UTR	55158579	55158910	55158579	ID=Msa1255850-mRNA-1:three_prime_utr;Parent=Msa1255850-mRNA-1

Gene Sequence

>Msa1255850

ATGCCGAACCCAAGAGTGTACTTCGACATAAGCATCGGCGGTGAACTCGAGGGTAGAATCGTAATCGAACTCTTCGCCGATGTCGTTCCCAAAACCGCCGAAAATTTCCGATCTCTCTGCACCGGCGAAAAAGGCATCGGTCCACACACCAACGTCCCTCTCCATTTCAAGAAATCCATCTTCCACCGTGTCGTCAAAGGCTTCATGATCCAAGGCGGTGACATCTCCGCCTCCGACGGAACCGGCGGCGAGTCAATCTACGGTCCAAATTTCGAAGACGAAAATTTCGACCTAAAACACGAACGGAAAGGGATTTTATCCATGGCGAATACCGGTCCAAACACCAATGGGAGTCAGTTTTTTATTACGACGACGAGGACGCCGCATTTGGATGGGAAGCATGTGGTGTTTGGGAAGGTTGTGAAAGGGATTGGAGTTGTTAGGTCTGTTGAGCTTGGTCCGGTTGGGGAGAATGATCGACCGGAGCAGGATGTTGTGATTGCTGATTGTGGGGAGATTGCTGAAGGGGAGGATGAAGGGGTGATTAACTTTTTTAAAGATGGTGATACTTTTGCTGATTGGCCTGTTGATCTTGATACCAAGCCGGAGGAGCTTGAATGGTGGATGAATGCTGTTGAATCTATCAAGGGCTTTGGAAATGAACACTATAAGAAGCAAGATTATAAGATGGCTATCAAAAAGTATCGCAAGGCTTTGCGCTATTTGGACATGTGTTGGGAGAAGGATGGTGTTGACCAAGAGAAGAGCACTGCTTTAAGGAAGATAAAATCTCAAATCTTTACCAACAGTTCGGCCTGTAAATTAAAATTAGGTGACCTCAGTGGAGCTTTACTTGATTCGGACTTTGCAATGCACGATGGAGATAATGCAAAAGCTCTGTTTCGCAAAGGCCAGGTGTATATGTTACTCAATGACCTGGATGCCGCTCTGGATAGCTTCAAGAGAGCATTAGAGTTGGAGCCAAATGATGGAGGGATAAAAAAAGAGTACGCAATTGCCAGGAAGAAGGTTGCTGATAGACATGATAAGGAGAAAAAAGCTTATTCGAAGATGTTCAATTAG

Protein sequence

>Msa1255850

MPNPRVYFDISIGGELEGRIVIELFADVVPKTAENFRSLCTGEKGIGPHTNVPLHFKKSIFHRVVKGFMIQGGDISASDGTGGESIYGPNFEDENFDLKHERKGILSMANTGPNTNGSQFFITTTRTPHLDGKHVVFGKVVKGIGVVRSVELGPVGENDRPEQDVVIADCGEIAEGEDEGVINFFKDGDTFADWPVDLDTKPEELEWWMNAVESIKGFGNEHYKKQDYKMAIKKYRKALRYLDMCWEKDGVDQEKSTALRKIKSQIFTNSSACKLKLGDLSGALLDSDFAMHDGDNAKALFRKGQVYMLLNDLDAALDSFKRALELEPNDGGIKKEYAIARKKVADRHDKEKKAYSKMFN*