AlfalfaGEDB | MS.gene56579

M. sativa cultivar XinJiangDaYe / MS.gene56579

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF08387.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56579.t1	XP_013464465.1	91.9	481	31	1	1	473	1	481	1.60E-246	861.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56579.t1	Q9LUC4	25.9	451	288	14	30	468	25	441	1.3e-23	112.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56579.t1	A0A072VK28	91.9	481	31	1	1	473	1	481	1.2e-246	861.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene36067	MS.gene56579	PPI
MS.gene25297	MS.gene56579	PPI
MS.gene34766	MS.gene56579	PPI
MS.gene56579	MS.gene63256	PPI
MS.gene72952	MS.gene56579	PPI
MS.gene56579	MS.gene032295	PPI
MS.gene061286	MS.gene56579	PPI
MS.gene065234	MS.gene56579	PPI
MS.gene34706	MS.gene56579	PPI
MS.gene34753	MS.gene56579	PPI
MS.gene72954	MS.gene56579	PPI
MS.gene060770	MS.gene56579	PPI
MS.gene053313	MS.gene56579	PPI
MS.gene72962	MS.gene56579	PPI
MS.gene25396	MS.gene56579	PPI
MS.gene055882	MS.gene56579	PPI
MS.gene99983	MS.gene56579	PPI
MS.gene49513	MS.gene56579	PPI
MS.gene69850	MS.gene56579	PPI
MS.gene64989	MS.gene56579	PPI
MS.gene63257	MS.gene56579	PPI
MS.gene055883	MS.gene56579	PPI
MS.gene062526	MS.gene56579	PPI
MS.gene63214	MS.gene56579	PPI
MS.gene25296	MS.gene56579	PPI
MS.gene055877	MS.gene56579	PPI
MS.gene63217	MS.gene56579	PPI
MS.gene032296	MS.gene56579	PPI
MS.gene25450	MS.gene56579	PPI
MS.gene34738	MS.gene56579	PPI
MS.gene56579	MS.gene25451	PPI
MS.gene056517	MS.gene56579	PPI
MS.gene34735	MS.gene56579	PPI
MS.gene63222	MS.gene56579	PPI
MS.gene34703	MS.gene56579	PPI
MS.gene63177	MS.gene56579	PPI
MS.gene25449	MS.gene56579	PPI
MS.gene060771	MS.gene56579	PPI
MS.gene34801	MS.gene56579	PPI
MS.gene63095	MS.gene56579	PPI
MS.gene72955	MS.gene56579	PPI
MS.gene63104	MS.gene56579	PPI
MS.gene99982	MS.gene56579	PPI
MS.gene34707	MS.gene56579	PPI
MS.gene56579	MS.gene72956	PPI
MS.gene63218	MS.gene56579	PPI
MS.gene93027	MS.gene56579	PPI
MS.gene63111	MS.gene56579	PPI
MS.gene34215	MS.gene56579	PPI
MS.gene062537	MS.gene56579	PPI
MS.gene073176	MS.gene56579	PPI
MS.gene34804	MS.gene56579	PPI
MS.gene25347	MS.gene56579	PPI
MS.gene34730	MS.gene56579	PPI
MS.gene48656	MS.gene56579	PPI
MS.gene63213	MS.gene56579	PPI
MS.gene63223	MS.gene56579	PPI
MS.gene34824	MS.gene56579	PPI
MS.gene63105	MS.gene56579	PPI
MS.gene64993	MS.gene56579	PPI
MS.gene72959	MS.gene56579	PPI
MS.gene72963	MS.gene56579	PPI
MS.gene36065	MS.gene56579	PPI
MS.gene25353	MS.gene56579	PPI
MS.gene56579	MS.gene34802	PPI
MS.gene25352	MS.gene56579	PPI
MS.gene92095	MS.gene56579	PPI
MS.gene25415	MS.gene56579	PPI
MS.gene63212	MS.gene56579	PPI
MS.gene36066	MS.gene56579	PPI
MS.gene56579	MS.gene95710	PPI
MS.gene055880	MS.gene56579	PPI
MS.gene043123	MS.gene56579	PPI
MS.gene25412	MS.gene56579	PPI
MS.gene25410	MS.gene56579	PPI
MS.gene25348	MS.gene56579	PPI
MS.gene63113	MS.gene56579	PPI
MS.gene63185	MS.gene56579	PPI
MS.gene34736	MS.gene56579	PPI
MS.gene56579	MS.gene64988	PPI
MS.gene63094	MS.gene56579	PPI
MS.gene053311	MS.gene56579	PPI
MS.gene63112	MS.gene56579	PPI
MS.gene72961	MS.gene56579	PPI
MS.gene34740	MS.gene56579	PPI
MS.gene64992	MS.gene56579	PPI
MS.gene63259	MS.gene56579	PPI
MS.gene56579	MS.gene25298	PPI
MS.gene56579	MS.gene25448	PPI
MS.gene25411	MS.gene56579	PPI
MS.gene25414	MS.gene56579	PPI
MS.gene34739	MS.gene56579	PPI
MS.gene34731	MS.gene56579	PPI
MS.gene062282	MS.gene56579	PPI
MS.gene053312	MS.gene56579	PPI
MS.gene007876	MS.gene56579	PPI
MS.gene25344	MS.gene56579	PPI
MS.gene25386	MS.gene56579	PPI
MS.gene065516	MS.gene56579	PPI
MS.gene34704	MS.gene56579	PPI
MS.gene34825	MS.gene56579	PPI
MS.gene61631	MS.gene56579	PPI
MS.gene34216	MS.gene56579	PPI
MS.gene48654	MS.gene56579	PPI
MS.gene34737	MS.gene56579	PPI
MS.gene067702	MS.gene56579	PPI
MS.gene66626	MS.gene56579	PPI
MS.gene56579	MS.gene017193	PPI
MS.gene56579	MS.gene63221	PPI
MS.gene64984	MS.gene56579	PPI
MS.gene72958	MS.gene56579	PPI
MS.gene63224	MS.gene56579	PPI
MS.gene007875	MS.gene56579	PPI
MS.gene25418	MS.gene56579	PPI
MS.gene63260	MS.gene56579	PPI
MS.gene038427	MS.gene56579	PPI
MS.gene72957	MS.gene56579	PPI
MS.gene25299	MS.gene56579	PPI
MS.gene060769	MS.gene56579	PPI
MS.gene56579	MS.gene34733	PPI
MS.gene043122	MS.gene56579	PPI
MS.gene63210	MS.gene56579	PPI
MS.gene56579	MS.gene99980	PPI
MS.gene72960	MS.gene56579	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56579.t1	MTR_2g072720	91.892	481	31	1	1	473	1	481	0.0	879
MS.gene56579.t1	MTR_2g072730	63.755	458	158	5	22	473	7	462	0.0	550
MS.gene56579.t1	MTR_4g032845	62.754	443	158	6	30	468	2	441	0.0	514
MS.gene56579.t1	MTR_1g025870	43.141	503	205	15	28	458	19	512	1.62e-97	303
MS.gene56579.t1	MTR_5g005700	41.509	477	232	14	30	471	30	494	1.13e-88	280
MS.gene56579.t1	MTR_6g022350	41.151	469	206	13	28	436	4	462	1.19e-87	276
MS.gene56579.t1	MTR_2g072710	65.946	185	59	1	289	473	18	198	4.92e-76	237
MS.gene56579.t1	MTR_5g007090	36.420	486	252	15	1	458	1	457	4.96e-71	234
MS.gene56579.t1	MTR_8g006910	40.097	414	217	14	21	424	40	432	1.27e-63	215
MS.gene56579.t1	MTR_2g072530	33.775	453	267	14	31	469	13	446	7.24e-60	203
MS.gene56579.t1	MTR_2g067210	39.119	386	202	14	13	378	31	403	1.51e-58	201
MS.gene56579.t1	MTR_7g094030	33.755	474	272	15	16	469	11	462	6.29e-58	198
MS.gene56579.t1	MTR_5g007130	33.619	467	212	17	29	470	12	405	2.42e-55	190
MS.gene56579.t1	MTR_2g072650	45.339	236	84	1	236	471	49	239	4.00e-55	184
MS.gene56579.t1	MTR_3g018680	35.570	447	210	18	21	457	10	388	3.29e-54	187
MS.gene56579.t1	MTR_1g115250	36.066	427	237	13	17	435	18	416	1.64e-53	186
MS.gene56579.t1	MTR_2g082800	34.670	424	237	14	11	422	6	401	2.91e-46	166
MS.gene56579.t1	MTR_2g082880	32.662	447	251	16	28	460	7	417	4.25e-43	157
MS.gene56579.t1	MTR_2g082850	33.602	372	210	12	21	374	12	364	2.74e-41	151
MS.gene56579.t1	MTR_1g115245	37.102	283	156	10	21	298	16	281	4.67e-35	133
MS.gene56579.t1	MTR_6g022330	33.705	359	155	12	40	337	28	364	7.65e-33	132
MS.gene56579.t1	MTR_5g007110	53.226	124	56	2	348	471	1	122	4.89e-32	119
MS.gene56579.t1	MTR_2g082780	35.294	238	120	11	13	241	1	213	4.80e-25	102
MS.gene56579.t1	MTR_4g080787	27.155	464	286	17	22	454	9	451	7.15e-25	107
MS.gene56579.t1	MTR_4g080773	26.364	440	279	15	22	432	9	432	9.72e-23	101
MS.gene56579.t1	MTR_4g080773	26.364	440	279	15	22	432	9	432	9.77e-23	101
MS.gene56579.t1	MTR_4g032730	25.553	497	274	21	28	468	10	466	9.06e-21	95.1
MS.gene56579.t1	MTR_4g080590	25.636	472	300	15	13	460	1	445	2.37e-20	94.0
MS.gene56579.t1	MTR_8g467800	26.707	498	274	18	18	471	5	455	5.43e-20	92.8
MS.gene56579.t1	MTR_7g094050	45.192	104	55	1	340	441	5	108	6.16e-18	80.5
MS.gene56579.t1	MTR_5g063450	30.584	291	169	10	31	319	27	286	2.67e-17	83.6
MS.gene56579.t1	MTR_5g005690	24.096	498	303	15	18	460	3	480	1.99e-16	82.0
MS.gene56579.t1	MTR_8g468810	23.922	510	298	19	1	468	1	462	2.27e-16	81.6
MS.gene56579.t1	MTR_5g006480	51.724	87	34	4	381	465	8	88	2.80e-15	72.0
MS.gene56579.t1	MTR_2g082860	41.414	99	47	3	11	104	4	96	1.05e-14	70.9
MS.gene56579.t1	MTR_2g048140	24.944	445	267	20	44	451	20	434	4.48e-14	74.7
MS.gene56579.t1	MTR_8g468840	24.625	467	279	15	31	461	14	443	7.85e-14	73.9
MS.gene56579.t1	MTR_3g048290	23.297	455	293	17	31	454	23	452	2.68e-13	72.4
MS.gene56579.t1	MTR_1g090907	25.383	457	267	21	31	446	7	430	2.76e-13	72.0
MS.gene56579.t1	MTR_2g047858	23.992	496	288	22	2	467	95	531	2.87e-13	72.4
MS.gene56579.t1	MTR_0049s0110	33.333	114	73	2	31	144	8	118	4.56e-13	69.7
MS.gene56579.t1	MTR_0049s0110	33.333	114	73	2	31	144	8	118	6.03e-13	70.1
MS.gene56579.t1	MTR_0049s0110	33.333	114	73	2	31	144	8	118	9.37e-13	69.7
MS.gene56579.t1	MTR_0049s0110	33.333	114	73	2	31	144	8	118	1.09e-12	70.1
MS.gene56579.t1	MTR_1g016310	25.000	320	186	12	15	313	4	290	1.86e-12	68.6
MS.gene56579.t1	MTR_4g080590	25.750	400	248	14	13	390	1	373	1.87e-12	68.9
MS.gene56579.t1	MTR_1g016310	25.000	320	186	12	15	313	4	290	2.89e-12	68.9
MS.gene56579.t1	MTR_8g015680	34.000	100	61	2	26	120	2	101	2.22e-11	66.2
MS.gene56579.t1	MTR_7g023310	24.138	435	281	14	21	432	17	425	2.35e-11	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56579.t1	AT3G14710	26.327	452	285	15	30	468	25	441	2.31e-26	111
MS.gene56579.t1	AT4G14096	27.040	429	264	17	31	432	8	414	2.45e-20	94.0
MS.gene56579.t1	AT4G14096	27.040	429	264	17	31	432	8	414	2.45e-20	94.0
MS.gene56579.t1	AT4G14103	26.932	427	242	15	31	432	8	389	3.37e-20	93.2
MS.gene56579.t1	AT4G14103	27.540	374	223	13	31	380	8	357	9.26e-20	91.3
MS.gene56579.t1	AT4G14103	27.540	374	223	13	31	380	8	357	9.26e-20	91.3
MS.gene56579.t1	AT3G59240	28.507	442	251	19	31	432	8	424	1.02e-18	89.4
MS.gene56579.t1	AT3G58940	42.982	114	63	1	31	142	2	115	5.33e-18	87.4
MS.gene56579.t1	AT3G58940	42.982	114	63	1	31	142	2	115	5.33e-18	87.4
MS.gene56579.t1	AT1G16930	24.779	452	276	15	19	435	2	424	5.65e-18	86.7
MS.gene56579.t1	AT1G16930	24.779	452	276	15	19	435	2	424	5.65e-18	86.7
MS.gene56579.t1	AT1G16930	24.779	452	276	15	19	435	2	424	5.65e-18	86.7
MS.gene56579.t1	AT3G58930	40.517	116	67	1	31	144	2	117	9.50e-18	86.3
MS.gene56579.t1	AT3G58930	40.517	116	67	1	31	144	2	117	9.50e-18	86.3
MS.gene56579.t1	AT3G58930	40.517	116	67	1	31	144	2	117	9.50e-18	86.3
MS.gene56579.t1	AT3G03040	32.243	214	124	7	31	233	2	205	1.33e-17	85.5
MS.gene56579.t1	AT5G38396	24.435	487	275	15	31	462	2	450	3.32e-17	84.3
MS.gene56579.t1	AT5G38392	32.143	196	123	3	31	219	2	194	4.81e-17	80.5
MS.gene56579.t1	AT3G59200	44.444	126	66	3	31	154	7	130	5.69e-17	82.8
MS.gene56579.t1	AT3G59200	44.444	126	66	3	31	154	7	130	2.04e-16	82.0
MS.gene56579.t1	AT3G58880	37.500	120	73	1	31	148	3	122	3.56e-16	81.3
MS.gene56579.t1	AT3G58900	40.517	116	67	1	31	144	2	117	7.34e-16	79.0
MS.gene56579.t1	AT4G13960	31.156	199	127	5	31	226	2	193	7.88e-16	80.1
MS.gene56579.t1	AT4G13960	31.156	199	127	5	31	226	2	193	7.88e-16	80.1
MS.gene56579.t1	AT3G58900	40.517	116	67	1	31	144	2	117	9.70e-16	78.6
MS.gene56579.t1	AT3G58900	40.517	116	67	1	31	144	2	117	9.70e-16	78.6
MS.gene56579.t1	AT5G56370	25.455	440	247	14	31	442	2	388	1.01e-15	79.3
MS.gene56579.t1	AT5G56370	25.455	440	247	14	31	442	2	388	1.01e-15	79.3
MS.gene56579.t1	AT3G58860	36.691	139	82	2	28	160	4	142	1.15e-15	79.7
MS.gene56579.t1	AT3G49150	40.171	117	66	2	29	144	14	127	1.19e-15	79.7
MS.gene56579.t1	AT5G22730	25.114	438	261	13	30	432	27	432	1.35e-15	79.3
MS.gene56579.t1	AT5G22730	25.114	438	261	13	30	432	27	432	1.35e-15	79.3
MS.gene56579.t1	AT3G59190	24.896	482	293	17	22	447	3	471	1.36e-15	79.7
MS.gene56579.t1	AT3G58900	40.517	116	67	1	31	144	2	117	1.54e-15	79.3
MS.gene56579.t1	AT3G59190	37.037	135	81	2	22	152	3	137	2.19e-15	78.2
MS.gene56579.t1	AT5G22670	28.355	462	258	22	31	459	11	432	4.57e-15	77.8
MS.gene56579.t1	AT3G03030	35.507	138	83	2	31	162	2	139	4.59e-15	76.6
MS.gene56579.t1	AT3G03030	35.507	138	83	2	31	162	2	139	5.25e-15	77.4
MS.gene56579.t1	AT3G58930	45.455	88	46	1	31	116	2	89	6.05e-15	77.4
MS.gene56579.t1	AT3G58930	45.455	88	46	1	31	116	2	89	6.05e-15	77.4
MS.gene56579.t1	AT3G58820	38.793	116	69	1	31	144	2	117	7.35e-15	76.6
MS.gene56579.t1	AT1G58310	27.540	443	247	16	31	415	8	434	1.54e-14	76.3
MS.gene56579.t1	AT1G69630	24.686	478	262	17	18	447	5	432	1.91e-14	75.9
MS.gene56579.t1	AT3G59230	42.500	120	65	2	31	146	12	131	2.60e-14	75.5
MS.gene56579.t1	AT5G22610	25.169	445	257	18	25	440	12	409	3.17e-14	75.1
MS.gene56579.t1	AT5G22610	25.110	454	276	17	25	440	12	439	4.03e-14	75.1
MS.gene56579.t1	AT3G59000	38.017	121	73	1	31	149	2	122	4.73e-14	73.6
MS.gene56579.t1	AT1G52650	37.069	116	71	1	31	144	2	117	6.43e-14	74.3
MS.gene56579.t1	AT3G59000	31.285	179	119	2	31	207	2	178	2.74e-13	72.4
MS.gene56579.t1	AT1G48400	31.544	149	96	3	31	176	10	155	9.39e-13	70.5
MS.gene56579.t1	AT1G48400	31.544	149	96	3	31	176	10	155	1.07e-12	70.5
MS.gene56579.t1	AT5G18780	26.018	442	255	16	24	432	4	406	1.12e-12	70.1
MS.gene56579.t1	AT5G18780	26.018	442	255	16	24	432	4	406	1.12e-12	70.1
MS.gene56579.t1	AT4G27050	25.298	419	249	16	31	408	4	399	1.18e-12	70.1
MS.gene56579.t1	AT4G27050	25.298	419	249	16	31	408	4	399	1.18e-12	70.1
MS.gene56579.t1	AT4G27050	25.298	419	249	16	31	408	4	399	1.18e-12	70.1
MS.gene56579.t1	AT4G27050	25.298	419	249	16	31	408	4	399	1.18e-12	70.1
MS.gene56579.t1	AT4G27050	25.298	419	249	16	31	408	4	399	1.18e-12	70.1
MS.gene56579.t1	AT4G13985	23.593	462	306	14	11	447	1	440	1.53e-12	69.7
MS.gene56579.t1	AT5G56370	25.203	369	207	11	31	381	2	319	1.62e-12	68.9
MS.gene56579.t1	AT5G41840	35.593	118	62	3	31	139	14	126	1.66e-12	70.1
MS.gene56579.t1	AT5G41630	35.294	119	75	1	31	147	12	130	3.80e-12	68.6
MS.gene56579.t1	AT3G50710	25.974	462	278	19	31	466	2	425	9.69e-12	67.4
MS.gene56579.t1	AT2G29910	29.146	199	117	6	31	223	5	185	2.32e-11	65.5
MS.gene56579.t1	AT2G29910	29.146	199	117	6	31	223	5	185	2.32e-11	65.5
MS.gene56579.t1	AT5G56440	24.886	438	253	18	31	434	2	397	2.53e-11	65.9
MS.gene56579.t1	AT5G41830	38.053	113	65	3	31	139	13	124	3.55e-11	65.9
MS.gene56579.t1	AT2G29910	29.146	199	117	6	31	223	5	185	3.73e-11	65.1
MS.gene56579.t1	AT2G29910	29.146	199	117	6	31	223	5	185	3.73e-11	65.1
MS.gene56579.t1	AT2G29910	29.146	199	117	6	31	223	5	185	3.73e-11	65.1
MS.gene56579.t1	AT2G29910	29.146	199	117	6	31	223	5	185	3.73e-11	65.1
MS.gene56579.t1	AT2G29910	29.146	199	117	6	31	223	29	209	4.74e-11	64.7

Find 63 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCTTCAAGTTGTGAAATT+TGG	0.084847	2.1:-25832634	MS.gene56579:CDS
ACCTGATTTCTTCTTACTTT+TGG	0.118640	2.1:+25834105	None:intergenic
CACAATAAGTGAATTCTTTC+AGG	0.138975	2.1:+25833549	None:intergenic
TTTCTTAGCTAAATATTTAA+TGG	0.167908	2.1:-25832584	MS.gene56579:CDS
GCTAAATATTTAATGGAAAA+TGG	0.194373	2.1:-25832577	MS.gene56579:CDS
TGCCGATATTATCATAGTTT+TGG	0.233216	2.1:-25833803	MS.gene56579:CDS
GGTTCTCACACGACCAAATA+TGG	0.293193	2.1:-25832922	MS.gene56579:CDS
ATTTGCAATGCTGACCCATT+TGG	0.331405	2.1:-25832886	MS.gene56579:CDS
ATGCTGACCCATTTGGATCT+TGG	0.336919	2.1:-25832879	MS.gene56579:CDS
ATCAAATGAAAATTCAAGTA+TGG	0.352381	2.1:+25832441	None:intergenic
TTAGGTAGAAGAGCCATATT+TGG	0.358781	2.1:+25832909	None:intergenic
GTGAAGTGTATGAAATTTCA+TGG	0.370274	2.1:-25833383	MS.gene56579:CDS
TAAATATAATTCCACACAAC+TGG	0.375749	2.1:+25833765	None:intergenic
TGCATGGCAGATTGATTCTA+AGG	0.376948	2.1:-25832518	MS.gene56579:CDS
TAGGAAAGCTGTTGTAGAAA+TGG	0.378123	2.1:-25834335	MS.gene56579:CDS
AATGCGAGATCTAAGTGAAT+AGG	0.379719	2.1:+25834243	None:intergenic
CACTAATTCTTCTAAATAAT+AGG	0.384805	2.1:+25833862	None:intergenic
TGGGTCAGCATTGCAAATTT+AGG	0.395407	2.1:+25832891	None:intergenic
ATTCTAGATCAAGAAATGAT+AGG	0.397342	2.1:-25834312	MS.gene56579:CDS
AATAATAGGCATTATTGAAA+AGG	0.403021	2.1:+25833876	None:intergenic
CCCAAAAGTAAGAAGAAATC+AGG	0.407003	2.1:-25834106	MS.gene56579:CDS
TTCCAAAACTATGATAATAT+CGG	0.419333	2.1:+25833801	None:intergenic
TGAGATGTAAGAGCAGAGAA+TGG	0.435630	2.1:+25833902	None:intergenic
CAAGAAATGATAGGGCTCAT+AGG	0.440489	2.1:-25834303	MS.gene56579:CDS
TAAGGTCATTGAAGAATTCA+AGG	0.449083	2.1:-25832500	MS.gene56579:CDS
AAAACTAGAGTATTGAGAAC+AGG	0.460237	2.1:+25832813	None:intergenic
ATGCGAGATCTAAGTGAATA+GGG	0.468509	2.1:+25834244	None:intergenic
CTGAAAGAATTCACTTATTG+TGG	0.476973	2.1:-25833548	MS.gene56579:CDS
GACCCATTTGGATCTTGGCT+CGG	0.486831	2.1:-25832874	MS.gene56579:CDS
ATAATAGGCATTATTGAAAA+GGG	0.487403	2.1:+25833877	None:intergenic
GGAAATAGTGTTCAAGAAAG+TGG	0.490116	2.1:-25833443	MS.gene56579:CDS
TAACCGAGCCAAGATCCAAA+TGG	0.494463	2.1:+25832871	None:intergenic
GAATTCACTTATTGTGGTGA+TGG	0.494488	2.1:-25833542	MS.gene56579:CDS
CAAGAACATGGATGGAAAGC+TGG	0.495167	2.1:-25834174	MS.gene56579:CDS
GCAACATTGCGAGCAGAAGA+TGG	0.503786	2.1:+25833491	None:intergenic
TTCTAGATCAAGAAATGATA+GGG	0.504637	2.1:-25834311	MS.gene56579:CDS
AGAGTCGATGCTAAACAATT+CGG	0.506897	2.1:+25832652	None:intergenic
CTTGGCTGAATTGATTGAGA+AGG	0.510794	2.1:+25833405	None:intergenic
GTGAAATTTGGAAATCTACA+TGG	0.531089	2.1:-25832622	MS.gene56579:CDS
GAAATTTCATACACTTCACT+TGG	0.537074	2.1:+25833387	None:intergenic
AAGTTGTACTCATTTAAGAA+AGG	0.540434	2.1:-25832475	MS.gene56579:CDS
AACCGAGCCAAGATCCAAAT+GGG	0.540980	2.1:+25832872	None:intergenic
TAGGGCTCATAGGAAGCAAA+AGG	0.553648	2.1:-25834293	MS.gene56579:CDS
CGAGTGTGTTATCAAGAACA+TGG	0.560378	2.1:-25834186	MS.gene56579:CDS
TGTGTTATCAAGAACATGGA+TGG	0.560875	2.1:-25834182	MS.gene56579:CDS
CTGGACATCAATCACCAAGT+TGG	0.569613	2.1:-25834155	MS.gene56579:CDS
TGATGTCACTTTAGAACTCA+AGG	0.570532	2.1:-25833663	MS.gene56579:CDS
GTGTATGAAATTTCATGGAT+CGG	0.573281	2.1:-25833378	MS.gene56579:CDS
ACTATCATCTAAGTCCAACT+TGG	0.576421	2.1:+25834141	None:intergenic
ATCAAACTCTTGTGAACACA+TGG	0.580028	2.1:-25833985	MS.gene56579:CDS
GTTTCTCCATTGCTAATGCA+TGG	0.584628	2.1:-25832534	MS.gene56579:CDS
AAGTTCTAAACTCGAAGCGA+TGG	0.585794	2.1:+25833925	None:intergenic
CTAAAACTCATCCAGTTGTG+TGG	0.598709	2.1:-25833776	MS.gene56579:CDS
AATCTGCCATGCATTAGCAA+TGG	0.618268	2.1:+25832528	None:intergenic
AAAAGTAAGAAGAAATCAGG+TGG	0.622127	2.1:-25834103	MS.gene56579:CDS
ATTGAGAAGGAGACAAGCAT+AGG	0.622398	2.1:+25833418	None:intergenic
GAGATCTAAGTGAATAGGGT+AGG	0.631025	2.1:+25834248	None:intergenic
TATAGAACAAGACCTTGAGT+CGG	0.638825	2.1:-25833618	MS.gene56579:CDS
GCTTAATGAGAATTATAGTG+TGG	0.643038	2.1:-25834041	MS.gene56579:CDS
GCTCACGAGTAACCGACTCA+AGG	0.644550	2.1:+25833606	None:intergenic
TATGAAATTTCATGGATCGG+AGG	0.662521	2.1:-25833375	MS.gene56579:intron
CTATGATAATATCGGCAGAG+AGG	0.671352	2.1:+25833809	None:intergenic
AAAATGGTATAGTGCTTGAG+AGG	0.676555	2.1:-25832561	MS.gene56579:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAATATAATAGAAATATAAA+AGG	-	chr2.1:25833486-25833505	MS.gene56579:CDS	10.0%
!!	TTTCTTAGCTAAATATTTAA+TGG	-	chr2.1:25834200-25834219	MS.gene56579:CDS	15.0%
!!	AATAATAGGCATTATTGAAA+AGG	+	chr2.1:25832911-25832930	None:intergenic	20.0%
!!	ATAATAGGCATTATTGAAAA+GGG	+	chr2.1:25832910-25832929	None:intergenic	20.0%
!!	ATCAAATGAAAATTCAAGTA+TGG	+	chr2.1:25834346-25834365	None:intergenic	20.0%
!!	ATTACTAATTACTAATCATC+AGG	-	chr2.1:25834065-25834084	MS.gene56579:CDS	20.0%
!!	CACTAATTCTTCTAAATAAT+AGG	+	chr2.1:25832925-25832944	None:intergenic	20.0%
!!	GCTAAATATTTAATGGAAAA+TGG	-	chr2.1:25834207-25834226	MS.gene56579:CDS	20.0%
!!	TGAAACAAAGTTTAAATAAG+AGG	+	chr2.1:25834046-25834065	None:intergenic	20.0%
!!	TGAAATATAAACGATTGTAA+AGG	+	chr2.1:25833563-25833582	None:intergenic	20.0%
!!	TTCATTCAATATGAATTCTA+AGG	-	chr2.1:25833579-25833598	MS.gene56579:CDS	20.0%
!!	TTCCAAAACTATGATAATAT+CGG	+	chr2.1:25832986-25833005	None:intergenic	20.0%
!!!	ATAAAAGGTTGTTAGTTTAT+TGG	-	chr2.1:25833501-25833520	MS.gene56579:CDS	20.0%
!!!	TTAAGGAAACTGATTTATAT+TGG	+	chr2.1:25833829-25833848	None:intergenic	20.0%
!!!	TTGATAGTTGTTTGTTTATA+GGG	-	chr2.1:25833524-25833543	MS.gene56579:CDS	20.0%
!	AAGTTGTACTCATTTAAGAA+AGG	-	chr2.1:25834309-25834328	MS.gene56579:CDS	25.0%
!	ATTCTAGATCAAGAAATGAT+AGG	-	chr2.1:25832472-25832491	MS.gene56579:CDS	25.0%
!	CAATATATTAGTCATTAGTG+TGG	+	chr2.1:25833708-25833727	None:intergenic	25.0%
!	TAAATATAATTCCACACAAC+TGG	+	chr2.1:25833022-25833041	None:intergenic	25.0%
!	TTCTAGATCAAGAAATGATA+GGG	-	chr2.1:25832473-25832492	MS.gene56579:CDS	25.0%
!!	AACATAATTTTGTATAAGCG+TGG	-	chr2.1:25833320-25833339	MS.gene56579:intron	25.0%
!!	ATAAAAACACTTTCAAGTAG+AGG	+	chr2.1:25833149-25833168	None:intergenic	25.0%
!!!	GTTGATAGTTGTTTGTTTAT+AGG	-	chr2.1:25833523-25833542	MS.gene56579:CDS	25.0%
	AAAAGTAAGAAGAAATCAGG+TGG	-	chr2.1:25832681-25832700	MS.gene56579:CDS	30.0%
	CACAATAAGTGAATTCTTTC+AGG	+	chr2.1:25833238-25833257	None:intergenic	30.0%
	CACGATTAACAACAAGATAT+TGG	+	chr2.1:25833685-25833704	None:intergenic	30.0%
	CTGAAAGAATTCACTTATTG+TGG	-	chr2.1:25833236-25833255	MS.gene56579:intron	30.0%
	GAAATTTCATACACTTCACT+TGG	+	chr2.1:25833400-25833419	None:intergenic	30.0%
	GCTTAATGAGAATTATAGTG+TGG	-	chr2.1:25832743-25832762	MS.gene56579:intron	30.0%
	GTGAAATTTGGAAATCTACA+TGG	-	chr2.1:25834162-25834181	MS.gene56579:CDS	30.0%
	GTGAAGTGTATGAAATTTCA+TGG	-	chr2.1:25833401-25833420	MS.gene56579:CDS	30.0%
	GTGTATGAAATTTCATGGAT+CGG	-	chr2.1:25833406-25833425	MS.gene56579:CDS	30.0%
	TAAGAGGAGATACAGATTAA+CGG	+	chr2.1:25834030-25834049	None:intergenic	30.0%
	TAAGGTCATTGAAGAATTCA+AGG	-	chr2.1:25834284-25834303	MS.gene56579:CDS	30.0%
	TAGATGAAGATTCATCTCAT+CGG	+	chr2.1:25833640-25833659	None:intergenic	30.0%
	TTGATACAAAAAACTGCTCT+TGG	-	chr2.1:25833087-25833106	MS.gene56579:intron	30.0%
!	ACCTGATTTCTTCTTACTTT+TGG	+	chr2.1:25832682-25832701	None:intergenic	30.0%
!	ATCTATAGAGATCCTTTTAC+CGG	-	chr2.1:25833052-25833071	MS.gene56579:intron	30.0%
!	TGCCGATATTATCATAGTTT+TGG	-	chr2.1:25832981-25833000	MS.gene56579:intron	30.0%
!!	AAAACTAGAGTATTGAGAAC+AGG	+	chr2.1:25833974-25833993	None:intergenic	30.0%
!!	AATTTGATTTTGCAGTTACG+TGG	+	chr2.1:25833203-25833222	None:intergenic	30.0%
!!	ACTCTTCAAGTTGTGAAATT+TGG	-	chr2.1:25834150-25834169	MS.gene56579:CDS	30.0%
!!	CCTGATTTCTTCTTACTTTT+GGG	+	chr2.1:25832681-25832700	None:intergenic	30.0%
!!!	AATACTCTAGTTTTCAAGGT+TGG	-	chr2.1:25833980-25833999	MS.gene56579:CDS	30.0%
!!!	GTATCAAACTTTTTCAGTAC+CGG	+	chr2.1:25833074-25833093	None:intergenic	30.0%
!!!	TCTCAATACTCTAGTTTTCA+AGG	-	chr2.1:25833976-25833995	MS.gene56579:CDS	30.0%
	AAAATGGTATAGTGCTTGAG+AGG	-	chr2.1:25834223-25834242	MS.gene56579:CDS	35.0%
	AACCTGGATGACAAACATTA+AGG	+	chr2.1:25833846-25833865	None:intergenic	35.0%
	AAGATTGAGAGCTTGATAGA+AGG	+	chr2.1:25833612-25833631	None:intergenic	35.0%
	AATGCGAGATCTAAGTGAAT+AGG	+	chr2.1:25832544-25832563	None:intergenic	35.0%
	ACTATCATCTAAGTCCAACT+TGG	+	chr2.1:25832646-25832665	None:intergenic	35.0%
	AGAGTCGATGCTAAACAATT+CGG	+	chr2.1:25834135-25834154	None:intergenic	35.0%
	AGATTGAGAGCTTGATAGAA+GGG	+	chr2.1:25833611-25833630	None:intergenic	35.0%
	AGCAATATTGTTGAGTGTCA+TGG	-	chr2.1:25833745-25833764	MS.gene56579:CDS	35.0%
	AGTGTCATGGCATAAATTTG+GGG	-	chr2.1:25833758-25833777	MS.gene56579:CDS	35.0%
	ATCAAACTCTTGTGAACACA+TGG	-	chr2.1:25832799-25832818	MS.gene56579:intron	35.0%
	ATGCGAGATCTAAGTGAATA+GGG	+	chr2.1:25832543-25832562	None:intergenic	35.0%
	CCCAAAAGTAAGAAGAAATC+AGG	-	chr2.1:25832678-25832697	MS.gene56579:CDS	35.0%
	GAATTCACTTATTGTGGTGA+TGG	-	chr2.1:25833242-25833261	MS.gene56579:intron	35.0%
	GAGTGTCATGGCATAAATTT+GGG	-	chr2.1:25833757-25833776	MS.gene56579:CDS	35.0%
	GGAAATAGTGTTCAAGAAAG+TGG	-	chr2.1:25833341-25833360	MS.gene56579:intron	35.0%
	TAGGAAAGCTGTTGTAGAAA+TGG	-	chr2.1:25832449-25832468	MS.gene56579:CDS	35.0%
	TATAGAACAAGACCTTGAGT+CGG	-	chr2.1:25833166-25833185	MS.gene56579:intron	35.0%
	TATGAAATTTCATGGATCGG+AGG	-	chr2.1:25833409-25833428	MS.gene56579:CDS	35.0%
	TGAGTGTCATGGCATAAATT+TGG	-	chr2.1:25833756-25833775	MS.gene56579:CDS	35.0%
	TGTGTTATCAAGAACATGGA+TGG	-	chr2.1:25832602-25832621	MS.gene56579:CDS	35.0%
	TTAGGTAGAAGAGCCATATT+TGG	+	chr2.1:25833878-25833897	None:intergenic	35.0%
	TTCCTTAATGTTTGTCATCC+AGG	-	chr2.1:25833841-25833860	MS.gene56579:CDS	35.0%
!!	TGATGTCACTTTAGAACTCA+AGG	-	chr2.1:25833121-25833140	MS.gene56579:intron	35.0%
!!!	ACTTTTTCAGTACCGGTAAA+AGG	+	chr2.1:25833067-25833086	None:intergenic	35.0%
	AATCTGCCATGCATTAGCAA+TGG	+	chr2.1:25834259-25834278	None:intergenic	40.0%
	ATTGAGAAGGAGACAAGCAT+AGG	+	chr2.1:25833369-25833388	None:intergenic	40.0%
	ATTTGCAATGCTGACCCATT+TGG	-	chr2.1:25833898-25833917	MS.gene56579:CDS	40.0%
	CAAGAAATGATAGGGCTCAT+AGG	-	chr2.1:25832481-25832500	MS.gene56579:CDS	40.0%
	CGAGTGTGTTATCAAGAACA+TGG	-	chr2.1:25832598-25832617	MS.gene56579:CDS	40.0%
	CTAAAACTCATCCAGTTGTG+TGG	-	chr2.1:25833008-25833027	MS.gene56579:intron	40.0%
	CTATGATAATATCGGCAGAG+AGG	+	chr2.1:25832978-25832997	None:intergenic	40.0%
	CTTGGCTGAATTGATTGAGA+AGG	+	chr2.1:25833382-25833401	None:intergenic	40.0%
	GAGATCTAAGTGAATAGGGT+AGG	+	chr2.1:25832539-25832558	None:intergenic	40.0%
	GATTGAGAGCTTGATAGAAG+GGG	+	chr2.1:25833610-25833629	None:intergenic	40.0%
	GTTTCTCCATTGCTAATGCA+TGG	-	chr2.1:25834250-25834269	MS.gene56579:CDS	40.0%
	TGAGATGTAAGAGCAGAGAA+TGG	+	chr2.1:25832885-25832904	None:intergenic	40.0%
	TGGGTCAGCATTGCAAATTT+AGG	+	chr2.1:25833896-25833915	None:intergenic	40.0%
!	TGCATGGCAGATTGATTCTA+AGG	-	chr2.1:25834266-25834285	MS.gene56579:CDS	40.0%
!!	AAGTTCTAAACTCGAAGCGA+TGG	+	chr2.1:25832862-25832881	None:intergenic	40.0%
	AACCGAGCCAAGATCCAAAT+GGG	+	chr2.1:25833915-25833934	None:intergenic	45.0%
	ATGCTGACCCATTTGGATCT+TGG	-	chr2.1:25833905-25833924	MS.gene56579:CDS	45.0%
	CAAGAACATGGATGGAAAGC+TGG	-	chr2.1:25832610-25832629	MS.gene56579:CDS	45.0%
	CTGGACATCAATCACCAAGT+TGG	-	chr2.1:25832629-25832648	MS.gene56579:CDS	45.0%
	GGTTCTCACACGACCAAATA+TGG	-	chr2.1:25833862-25833881	MS.gene56579:CDS	45.0%
	TAACCGAGCCAAGATCCAAA+TGG	+	chr2.1:25833916-25833935	None:intergenic	45.0%
	TAGGGCTCATAGGAAGCAAA+AGG	-	chr2.1:25832491-25832510	MS.gene56579:CDS	45.0%
	TATTTGGTCGTGTGAGAACC+TGG	+	chr2.1:25833862-25833881	None:intergenic	45.0%
!	GTACGAGCAGCATCTTTTGT+CGG	+	chr2.1:25832579-25832598	None:intergenic	45.0%
!	TACGAGCAGCATCTTTTGTC+GGG	+	chr2.1:25832578-25832597	None:intergenic	45.0%
	GACCCATTTGGATCTTGGCT+CGG	-	chr2.1:25833910-25833929	MS.gene56579:CDS	50.0%
	GCAACATTGCGAGCAGAAGA+TGG	+	chr2.1:25833296-25833315	None:intergenic	50.0%
!	GCTCACGAGTAACCGACTCA+AGG	+	chr2.1:25833181-25833200	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	25832432	25834374	25832432	ID=MS.gene56579
chr2.1	mRNA	25832432	25834374	25832432	ID=MS.gene56579.t1;Parent=MS.gene56579
chr2.1	exon	25833376	25834374	25833376	ID=MS.gene56579.t1.exon1;Parent=MS.gene56579.t1
chr2.1	CDS	25833376	25834374	25833376	ID=cds.MS.gene56579.t1;Parent=MS.gene56579.t1
chr2.1	exon	25832809	25832943	25832809	ID=MS.gene56579.t1.exon2;Parent=MS.gene56579.t1
chr2.1	CDS	25832809	25832943	25832809	ID=cds.MS.gene56579.t1;Parent=MS.gene56579.t1
chr2.1	exon	25832432	25832719	25832432	ID=MS.gene56579.t1.exon3;Parent=MS.gene56579.t1
chr2.1	CDS	25832432	25832719	25832432	ID=cds.MS.gene56579.t1;Parent=MS.gene56579.t1

Gene Sequence

>MS.gene56579

ATGCAGAAAAATAGTTCTAGGAAAGCTGTTGTAGAAATGGATTCTAGATCAAGAAATGATAGGGCTCATAGGAAGCAAAAGGAGAAAGATGATATAATCAGCAACCTACCCTATTCACTTAGATCTCGCATTCTCTCATTTCTCCCGACAAAAGATGCTGCTCGTACGAGTGTGTTATCAAGAACATGGATGGAAAGCTGGACATCAATCACCAAGTTGGACTTAGATGATAGTGTTTTCTATTCTCCCAAAAGTAAGAAGAAATCAGGTGGAAAACAACACTTTATAAACTTTGTCAATAGAGCACTTCTGCTTAATGAGAATTATAGTGTGGAATGTTTTTCTCTTGTGATCGCTAATAAGTATGATCAAACTCTTGTGAACACATGGATCTCTTGTATGTTGAAGAAAGATGTAAAAAAACTTTCCATCGCTTCGAGTTTAGAACTTCCATTCTCTGCTCTTACATCTCACTCCCTTTTCAATAATGCCTATTATTTAGAAGAATTAGTGCTCAAGATGCATTGTTGTGCTCTCAAAGTTCCTCTCTGCCGATATTATCATAGTTTTGGAAAGCTAAAACTCATCCAGTTGTGTGGAATTATATTTACAATTGACAAATCTATAGAGATCCTTTTACCGGTACTGAAAAAGTTTGATACAAAAAACTGCTCTTGGTTAAGTGCACATGATGTCACTTTAGAACTCAAGGCACCTCTACTTGAAAGTGTTTTTATAGAACAAGACCTTGAGTCGGTTACTCGTGAGCCACGTAACTGCAAAATCAAATTTTCTGATTCAAGCCTGAAAGAATTCACTTATTGTGGTGATGGTATATCACAAGCTATTGTTCTGTCAGATCCATCTTCTGCTCGCAATGTTGCTGCTAACATAATTTTGTATAAGCGTGGAAATAGTGTTCAAGAAAGTGGATCCTATGCTTGTCTCCTTCTCAATCAATTCAGCCAAGTGAAGTGTATGAAATTTCATGGATCGGAGGTAAGATAATTTCATTCAACTTGCTTAATCTAATACTTAATATGTTGATGACATTGAATATAATAGAAATATAAAAGGTTGTTAGTTTATTGGTTGATAGTTGTTTGTTTATAGGGCATCTAATGTATTCCTTTACAATCGTTTATATTTCATTCAATATGAATTCTAAGGAATTACCCCTTCTATCAAGCTCTCAATCTTATTCTCCGATGAGATGAATCTTCATCTATCTGATTGTGCGACTTGTAACACCAATATCTTGTTGTTAATCGTGCCACACTAATGACTAATATATTGATTTGATTTATGAAGTTAGCAATATTGTTGAGTGTCATGGCATAAATTTGGGGTGTGTTTATTCGCATACATAGCTACATTTAAACATAGATGTTTTTCCAATATAAATCAGTTTCCTTAATGTTTGTCATCCAGGTTCTCACACGACCAAATATGGCTCTTCTACCTAAATTTGCAATGCTGACCCATTTGGATCTTGGCTCGGTTAGTGATGAAGTTTTGTTGTGCTTACTTCAAAAGTCTCCTGTTCTCAATACTCTAGTTTTCAAGGTTGGTAATTATATTGCATTATGCTTTAACCGTTAATCTGTATCTCCTCTTATTTAAACTTTGTTTCATTACTAATTACTAATCATCAGGAAATTACAAAATGCGATCAAGAGCGTCTAAGTTCTTATGTTGTGCCGAATTGTTTAGCATCGACTCTTCAAGTTGTGAAATTTGGAAATCTACATGGACACGAACATGAGCTTTTCTTAGCTAAATATTTAATGGAAAATGGTATAGTGCTTGAGAGGATGAGTTTCTCCATTGCTAATGCATGGCAGATTGATTCTAAGGTCATTGAAGAATTCAAGGAGAAGTTGTACTCATTTAAGAAAGGAGTCTCTTTTGCCATACTTGAATTTTCATTTGATTATTATTAG

Protein sequence

>MS.gene56579.t1

MQKNSSRKAVVEMDSRSRNDRAHRKQKEKDDIISNLPYSLRSRILSFLPTKDAARTSVLSRTWMESWTSITKLDLDDSVFYSPKSKKKSGGKQHFINFVNRALLLNENYSVECFSLVIANKYDQTLVNTWISCMLKKDVKKLSIASSLELPFSALTSHSLFNNAYYLEELVLKMHCCALKVPLCRYYHSFGKLKLIQLCGIIFTIDKSIEILLPVLKKFDTKNCSWLSAHDVTLELKAPLLESVFIEQDLESVTREPRNCKIKFSDSSLKEFTYCGDGISQAIVLSDPSSARNVAANIILYKRGNSVQESGSYACLLLNQFSQVKCMKFHGSEVLTRPNMALLPKFAMLTHLDLGSVSDEVLLCLLQKSPVLNTLVFKEITKCDQERLSSYVVPNCLASTLQVVKFGNLHGHEHELFLAKYLMENGIVLERMSFSIANAWQIDSKVIEEFKEKLYSFKKGVSFAILEFSFDYY