AlfalfaGEDB | MS.gene95994

M. sativa cultivar XinJiangDaYe / MS.gene95994

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95994.t1	XP_013464451.1	85.1	437	60	2	1	433	14	449	1.00E-207	732.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95994.t1	Q9LUC4	31.0	442	261	12	3	429	29	441	3.7e-36	154.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95994.t1	A0A072VA50	85.1	437	60	2	1	433	14	449	7.4e-208	732.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene34703	MS.gene95994	PPI
MS.gene63177	MS.gene95994	PPI
MS.gene25449	MS.gene95994	PPI
MS.gene95994	MS.gene25298	PPI
MS.gene95994	MS.gene25448	PPI
MS.gene34801	MS.gene95994	PPI
MS.gene63095	MS.gene95994	PPI
MS.gene72955	MS.gene95994	PPI
MS.gene63104	MS.gene95994	PPI
MS.gene043123	MS.gene95994	PPI
MS.gene95994	MS.gene34740	PPI
MS.gene95994	MS.gene92095	PPI
MS.gene63113	MS.gene95994	PPI
MS.gene34707	MS.gene95994	PPI
MS.gene93027	MS.gene95994	PPI
MS.gene34215	MS.gene95994	PPI
MS.gene062537	MS.gene95994	PPI
MS.gene34804	MS.gene95994	PPI
MS.gene95994	MS.gene062526	PPI
MS.gene95994	MS.gene64992	PPI
MS.gene95994	MS.gene060770	PPI
MS.gene25347	MS.gene95994	PPI
MS.gene48656	MS.gene95994	PPI
MS.gene95994	MS.gene34736	PPI
MS.gene63213	MS.gene95994	PPI
MS.gene63223	MS.gene95994	PPI
MS.gene34824	MS.gene95994	PPI
MS.gene64993	MS.gene95994	PPI
MS.gene95994	MS.gene63222	PPI
MS.gene95994	MS.gene34730	PPI
MS.gene95994	MS.gene25418	PPI
MS.gene72959	MS.gene95994	PPI
MS.gene34731	MS.gene95994	PPI
MS.gene062282	MS.gene95994	PPI
MS.gene95994	MS.gene34733	PPI
MS.gene25353	MS.gene95994	PPI
MS.gene065516	MS.gene95994	PPI
MS.gene25352	MS.gene95994	PPI
MS.gene34704	MS.gene95994	PPI
MS.gene95994	MS.gene34825	PPI
MS.gene95994	MS.gene25296	PPI
MS.gene95994	MS.gene99980	PPI
MS.gene25415	MS.gene95994	PPI
MS.gene95994	MS.gene25297	PPI
MS.gene95994	MS.gene25451	PPI
MS.gene48654	MS.gene95994	PPI
MS.gene95994	MS.gene043122	PPI
MS.gene067702	MS.gene95994	PPI
MS.gene63212	MS.gene95994	PPI
MS.gene36066	MS.gene95994	PPI
MS.gene055880	MS.gene95994	PPI
MS.gene95994	MS.gene63256	PPI
MS.gene25412	MS.gene95994	PPI
MS.gene25410	MS.gene95994	PPI
MS.gene95994	MS.gene032295	PPI
MS.gene63185	MS.gene95994	PPI
MS.gene63094	MS.gene95994	PPI
MS.gene038427	MS.gene95994	PPI
MS.gene95994	MS.gene007875	PPI
MS.gene63112	MS.gene95994	PPI
MS.gene95994	MS.gene72956	PPI
MS.gene95994	MS.gene72961	PPI
MS.gene72960	MS.gene95994	PPI
MS.gene25411	MS.gene95994	PPI
MS.gene25414	MS.gene95994	PPI
MS.gene34739	MS.gene95994	PPI
MS.gene053312	MS.gene95994	PPI
MS.gene25344	MS.gene95994	PPI
MS.gene34753	MS.gene95994	PPI
MS.gene61631	MS.gene95994	PPI
MS.gene95994	MS.gene63218	PPI
MS.gene34216	MS.gene95994	PPI
MS.gene95994	MS.gene34802	PPI
MS.gene66626	MS.gene95994	PPI
MS.gene64984	MS.gene95994	PPI
MS.gene95994	MS.gene073176	PPI
MS.gene95994	MS.gene72952	PPI
MS.gene72958	MS.gene95994	PPI
MS.gene055882	MS.gene95994	PPI
MS.gene63224	MS.gene95994	PPI
MS.gene95994	MS.gene34766	PPI
MS.gene99983	MS.gene95994	PPI
MS.gene63260	MS.gene95994	PPI
MS.gene72957	MS.gene95994	PPI
MS.gene25299	MS.gene95994	PPI
MS.gene060769	MS.gene95994	PPI
MS.gene63210	MS.gene95994	PPI
MS.gene63217	MS.gene95994	PPI
MS.gene95994	MS.gene053311	PPI
MS.gene95994	MS.gene63105	PPI
MS.gene36067	MS.gene95994	PPI
MS.gene95994	MS.gene63259	PPI
MS.gene060771	MS.gene95994	PPI
MS.gene061286	MS.gene95994	PPI
MS.gene065234	MS.gene95994	PPI
MS.gene34706	MS.gene95994	PPI
MS.gene95994	MS.gene007876	PPI
MS.gene99982	MS.gene95994	PPI
MS.gene95994	MS.gene34737	PPI
MS.gene72954	MS.gene95994	PPI
MS.gene053313	MS.gene95994	PPI
MS.gene95994	MS.gene25348	PPI
MS.gene72962	MS.gene95994	PPI
MS.gene95994	MS.gene36065	PPI
MS.gene25396	MS.gene95994	PPI
MS.gene63111	MS.gene95994	PPI
MS.gene95994	MS.gene017193	PPI
MS.gene95994	MS.gene95710	PPI
MS.gene95994	MS.gene25386	PPI
MS.gene95994	MS.gene63221	PPI
MS.gene95994	MS.gene25450	PPI
MS.gene49513	MS.gene95994	PPI
MS.gene69850	MS.gene95994	PPI
MS.gene95994	MS.gene64988	PPI
MS.gene64989	MS.gene95994	PPI
MS.gene63257	MS.gene95994	PPI
MS.gene055883	MS.gene95994	PPI
MS.gene95994	MS.gene055877	PPI
MS.gene63214	MS.gene95994	PPI
MS.gene032296	MS.gene95994	PPI
MS.gene34738	MS.gene95994	PPI
MS.gene72963	MS.gene95994	PPI
MS.gene056517	MS.gene95994	PPI
MS.gene34735	MS.gene95994	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95994.t1	MTR_2g072530	85.126	437	60	2	1	433	14	449	0.0	755
MS.gene95994.t1	MTR_2g072550	82.258	124	22	0	172	295	31	154	7.05e-66	210
MS.gene95994.t1	MTR_7g094030	34.169	439	252	13	13	430	40	462	8.60e-63	210
MS.gene95994.t1	MTR_2g082880	34.152	407	240	11	1	395	11	401	2.62e-61	205
MS.gene95994.t1	MTR_2g082800	36.198	384	232	11	1	382	28	400	4.75e-55	188
MS.gene95994.t1	MTR_2g072720	34.146	451	246	16	10	430	48	477	2.83e-54	188
MS.gene95994.t1	MTR_2g072730	34.694	441	255	17	1	420	17	445	2.73e-51	179
MS.gene95994.t1	MTR_1g115250	31.628	430	250	13	1	415	33	433	3.05e-51	179
MS.gene95994.t1	MTR_2g082850	35.150	367	205	14	1	349	23	374	9.10e-49	171
MS.gene95994.t1	MTR_4g032845	30.882	408	261	11	1	393	4	405	1.62e-45	164
MS.gene95994.t1	MTR_1g025870	31.368	424	254	15	12	414	96	503	8.37e-38	144
MS.gene95994.t1	MTR_2g082780	40.299	201	115	5	5	204	17	213	1.77e-36	133
MS.gene95994.t1	MTR_6g022350	27.542	472	257	16	14	415	20	476	6.83e-32	127
MS.gene95994.t1	MTR_2g067210	32.507	363	206	14	3	339	51	400	6.54e-31	124
MS.gene95994.t1	MTR_5g005700	29.231	455	267	14	2	415	33	473	7.97e-29	118
MS.gene95994.t1	MTR_5g007090	29.263	434	260	17	13	418	42	456	6.10e-27	113
MS.gene95994.t1	MTR_5g005690	25.322	466	289	11	12	428	32	487	1.23e-26	112
MS.gene95994.t1	MTR_1g115245	32.946	258	150	9	1	249	27	270	9.62e-26	106
MS.gene95994.t1	MTR_4g080590	27.674	430	288	13	1	418	24	442	1.28e-24	105
MS.gene95994.t1	MTR_8g006910	28.713	404	235	17	5	384	57	431	2.42e-23	102
MS.gene95994.t1	MTR_4g080787	26.105	475	274	17	1	429	20	463	6.05e-23	101
MS.gene95994.t1	MTR_5g007130	27.228	404	209	14	13	393	27	368	4.30e-22	98.2
MS.gene95994.t1	MTR_5g063450	30.795	302	161	12	2	291	29	294	2.59e-21	95.1
MS.gene95994.t1	MTR_3g048290	26.362	459	294	16	1	431	24	466	7.14e-21	95.1
MS.gene95994.t1	MTR_5g084070	24.153	443	286	13	2	427	15	424	1.25e-20	94.0
MS.gene95994.t1	MTR_4g080773	25.442	452	276	14	1	413	20	449	4.51e-19	89.7
MS.gene95994.t1	MTR_4g080773	25.442	452	276	14	1	413	20	449	5.05e-19	89.7
MS.gene95994.t1	MTR_5g039950	25.670	448	262	17	1	429	24	419	5.28e-19	89.0
MS.gene95994.t1	MTR_7g023310	25.000	416	282	14	1	398	27	430	8.52e-19	88.6
MS.gene95994.t1	MTR_3g018680	25.122	410	221	16	5	396	27	368	8.91e-18	85.1
MS.gene95994.t1	MTR_4g081030	28.443	334	195	13	2	319	19	324	1.15e-16	81.6
MS.gene95994.t1	MTR_2g072710	28.947	190	115	5	250	431	18	195	6.95e-16	76.3
MS.gene95994.t1	MTR_0049s0110	25.173	433	241	19	1	400	9	391	9.09e-15	76.3
MS.gene95994.t1	MTR_4g080590	26.796	362	244	13	1	352	24	374	1.31e-14	75.5
MS.gene95994.t1	MTR_0049s0110	26.111	360	201	16	1	342	9	321	3.14e-12	67.8
MS.gene95994.t1	MTR_8g467800	23.696	460	290	18	2	429	22	452	3.19e-12	68.6
MS.gene95994.t1	MTR_0049s0110	26.111	360	201	16	1	342	9	321	3.57e-12	67.8
MS.gene95994.t1	MTR_7g094050	34.821	112	68	1	301	407	2	113	1.48e-11	62.0
MS.gene95994.t1	MTR_8g062080	25.000	460	273	18	2	429	25	444	5.06e-11	64.7
MS.gene95994.t1	MTR_3g105060	30.570	193	105	8	2	182	16	191	5.52e-11	64.3
MS.gene95994.t1	MTR_2g082860	48.529	68	33	2	1	68	28	93	8.90e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95994.t1	AT3G14710	30.995	442	261	12	3	429	29	441	2.21e-42	155
MS.gene95994.t1	AT4G10400	25.792	442	279	13	2	430	4	409	6.35e-19	88.6
MS.gene95994.t1	AT4G10400	25.792	442	279	13	2	430	4	409	6.35e-19	88.6
MS.gene95994.t1	AT4G10400	25.792	442	279	13	2	430	4	409	6.35e-19	88.6
MS.gene95994.t1	AT4G14096	27.401	354	225	12	1	339	9	345	3.90e-17	83.6
MS.gene95994.t1	AT4G14096	27.401	354	225	12	1	339	9	345	3.90e-17	83.6
MS.gene95994.t1	AT3G03030	25.404	433	277	16	1	395	3	427	5.00e-17	83.6
MS.gene95994.t1	AT3G59200	26.344	372	221	13	2	339	9	361	4.11e-16	79.7
MS.gene95994.t1	AT4G26340	25.223	448	282	14	2	429	4	418	6.55e-16	79.7
MS.gene95994.t1	AT3G59200	26.344	372	221	13	2	339	9	361	1.60e-15	79.0
MS.gene95994.t1	AT4G14103	26.870	361	225	14	1	339	9	352	2.73e-15	77.4
MS.gene95994.t1	AT4G14103	26.870	361	225	14	1	339	9	352	2.73e-15	77.4
MS.gene95994.t1	AT3G03030	26.403	303	193	11	13	291	14	310	3.25e-15	77.0
MS.gene95994.t1	AT3G42770	27.212	452	254	21	13	428	11	423	3.36e-15	78.2
MS.gene95994.t1	AT4G14103	26.870	361	225	14	1	339	9	352	5.10e-15	77.0
MS.gene95994.t1	AT5G38590	25.243	412	262	12	2	397	4	385	1.06e-14	75.9
MS.gene95994.t1	AT2G42730	25.847	472	278	20	1	428	6	449	1.79e-14	75.5
MS.gene95994.t1	AT4G09920	33.333	165	100	4	2	162	4	162	2.56e-14	73.9
MS.gene95994.t1	AT4G09920	33.333	165	100	4	2	162	4	162	2.56e-14	73.9
MS.gene95994.t1	AT4G09920	31.892	185	114	5	2	182	4	180	2.71e-14	73.6
MS.gene95994.t1	AT4G09920	31.892	185	114	5	2	182	4	180	2.71e-14	73.6
MS.gene95994.t1	AT4G09920	31.892	185	114	5	2	182	4	180	2.71e-14	73.6
MS.gene95994.t1	AT5G35995	24.131	489	269	17	2	429	7	454	6.36e-14	73.9
MS.gene95994.t1	AT5G35995	24.131	489	269	17	2	429	7	454	6.36e-14	73.9
MS.gene95994.t1	AT5G56560	23.702	443	291	15	2	429	7	417	7.57e-14	73.6
MS.gene95994.t1	AT3G59240	26.705	352	235	12	1	339	9	350	8.64e-14	73.6
MS.gene95994.t1	AT2G26860	25.120	418	248	16	2	387	4	388	9.22e-14	73.2
MS.gene95994.t1	AT2G26860	25.120	418	248	16	2	387	4	388	9.22e-14	73.2
MS.gene95994.t1	AT3G58930	25.199	377	239	14	2	344	4	371	1.23e-13	73.2
MS.gene95994.t1	AT3G58930	25.199	377	239	14	2	344	4	371	1.23e-13	73.2
MS.gene95994.t1	AT3G58930	25.199	377	239	14	2	344	4	371	1.23e-13	73.2
MS.gene95994.t1	AT4G27050	24.699	498	256	23	1	429	5	452	2.93e-13	71.6
MS.gene95994.t1	AT4G27050	24.699	498	256	23	1	429	5	452	2.93e-13	71.6
MS.gene95994.t1	AT4G27050	24.699	498	256	23	1	429	5	452	2.93e-13	71.6
MS.gene95994.t1	AT4G27050	24.699	498	256	23	1	429	5	452	2.93e-13	71.6
MS.gene95994.t1	AT4G27050	24.699	498	256	23	1	429	5	452	2.93e-13	71.6
MS.gene95994.t1	AT3G03040	24.318	440	278	15	14	416	15	436	4.95e-13	71.2
MS.gene95994.t1	AT1G58310	26.984	378	225	15	1	339	9	374	6.31e-13	70.9
MS.gene95994.t1	AT5G56410	24.634	410	250	15	14	387	16	402	1.16e-12	70.1
MS.gene95994.t1	AT3G58900	40.171	117	60	5	13	121	14	128	3.72e-12	67.4
MS.gene95994.t1	AT3G58940	34.356	163	88	7	13	158	14	174	4.71e-12	68.6
MS.gene95994.t1	AT3G58940	34.356	163	88	7	13	158	14	174	4.71e-12	68.6
MS.gene95994.t1	AT3G60040	26.343	391	250	15	2	369	234	609	5.64e-12	68.2
MS.gene95994.t1	AT3G58900	38.760	129	68	6	1	121	3	128	5.98e-12	67.0
MS.gene95994.t1	AT3G58900	38.760	129	68	6	1	121	3	128	5.98e-12	67.0
MS.gene95994.t1	AT3G60040	26.343	391	250	15	2	369	232	607	6.04e-12	68.2
MS.gene95994.t1	AT3G58900	40.171	117	60	5	13	121	14	128	6.92e-12	67.4
MS.gene95994.t1	AT3G60040	26.343	391	250	15	2	369	15	390	7.33e-12	67.8
MS.gene95994.t1	AT3G60040	26.343	391	250	15	2	369	234	609	7.93e-12	67.8
MS.gene95994.t1	AT3G60040	26.343	391	250	15	2	369	235	610	8.00e-12	67.8
MS.gene95994.t1	AT4G10410	30.636	173	106	4	15	185	1	161	8.12e-12	65.9
MS.gene95994.t1	AT4G00160	23.747	459	284	16	2	429	18	441	2.92e-11	65.5
MS.gene95994.t1	AT4G13960	33.908	174	102	6	2	166	4	173	4.12e-11	65.1
MS.gene95994.t1	AT4G13960	33.908	174	102	6	2	166	4	173	4.12e-11	65.1
MS.gene95994.t1	AT5G41630	35.043	117	69	3	1	112	13	127	6.30e-11	64.7
MS.gene95994.t1	AT4G13965	24.703	421	253	16	2	397	12	393	6.62e-11	64.3
MS.gene95994.t1	AT5G22610	23.656	465	299	18	1	428	19	464	7.17e-11	64.3
MS.gene95994.t1	AT1G55030	25.542	415	248	20	1	392	20	396	8.94e-11	63.9
MS.gene95994.t1	AT1G06630	26.374	364	226	15	2	345	23	364	9.23e-11	63.9

Find 53 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAAAGTATTTCAGTTTAA+AGG	0.139696	2.4:-23663973	MS.gene95994:CDS
GCGTCGACTATGAACTTTAA+TGG	0.252172	2.4:+23664873	None:intergenic
ACTTTACATGAAAGCATTCT+TGG	0.257982	2.4:-23665531	MS.gene95994:CDS
TACATGATTCATTTGATTGA+TGG	0.283828	2.4:+23664902	None:intergenic
AAACAAGAGTGTTAAGAATA+GGG	0.284563	2.4:+23664370	None:intergenic
CAAGAAGATGGATTGAAGTT+TGG	0.301616	2.4:-23665452	MS.gene95994:CDS
ATCAAACTTTAGTCTGGTTA+TGG	0.303311	2.4:+23665425	None:intergenic
TGTTGTTGATGAAGATAAAA+AGG	0.327183	2.4:-23664764	MS.gene95994:CDS
GCGATATGCAAATATTGATT+TGG	0.330397	2.4:-23663854	MS.gene95994:CDS
GTGCTTACCGCTTCTATGAT+TGG	0.347239	2.4:+23665486	None:intergenic
AAAACAAGAGTGTTAAGAAT+AGG	0.349193	2.4:+23664369	None:intergenic
TTCACCAATCTGCCACTCTT+TGG	0.354370	2.4:-23664459	MS.gene95994:CDS
TCTGAGATGCATCATATTGA+TGG	0.386940	2.4:+23665280	None:intergenic
CCTAACAGATTTCTCTTATG+AGG	0.396373	2.4:-23664851	MS.gene95994:CDS
GCTTCAAACACTTTGAGAAT+GGG	0.416926	2.4:+23665006	None:intergenic
GTTGGCAAATGCTGAGATCT+TGG	0.419863	2.4:-23663908	MS.gene95994:CDS
AGCTTCAAACACTTTGAGAA+TGG	0.423087	2.4:+23665005	None:intergenic
ACTATCATCAAACTTTAGTC+TGG	0.424654	2.4:+23665419	None:intergenic
AAACTAATGTACTGTGTTGT+TGG	0.431228	2.4:+23664967	None:intergenic
CATCGAAAAGGTGAATTTGA+AGG	0.437469	2.4:+23665180	None:intergenic
CCTTCCAAAGAGTGGCAGAT+TGG	0.439202	2.4:+23664455	None:intergenic
CTATGATTGGAATGAAAGAT+AGG	0.445670	2.4:+23665499	None:intergenic
CTCAAAGTGTTTGAAGCTAG+AGG	0.446938	2.4:-23665000	MS.gene95994:CDS
GGATTTGCTTTATCTTAGAA+AGG	0.458843	2.4:-23665248	MS.gene95994:CDS
TTAAGCAAAGGGAGAAACTT+AGG	0.479350	2.4:+23665307	None:intergenic
AAGTTTGATGATAGTTTGCT+TGG	0.483093	2.4:-23665411	MS.gene95994:CDS
CTAGCGTCTTATCAAGAAGA+TGG	0.494487	2.4:-23665464	MS.gene95994:CDS
ATTGAAAACTTCTCAAGTAA+AGG	0.498014	2.4:+23664937	None:intergenic
ACTAGAGAATTGCATCGAAA+AGG	0.511379	2.4:+23665168	None:intergenic
TTGCAGTTGTTAGAACACTT+TGG	0.513678	2.4:+23663802	None:intergenic
GGATAAGATGAAGATATTGT+CGG	0.514578	2.4:-23663887	MS.gene95994:CDS
CCTCATAAGAGAAATCTGTT+AGG	0.515819	2.4:+23664851	None:intergenic
AACTTATCTGCAACTAAATG+AGG	0.518407	2.4:-23664430	MS.gene95994:CDS
AAGGATAGAATGGAAAAGCT+AGG	0.522063	2.4:-23664745	MS.gene95994:CDS
CCAATCTGCCACTCTTTGGA+AGG	0.524604	2.4:-23664455	MS.gene95994:CDS
AACAAGAGTGTTAAGAATAG+GGG	0.527875	2.4:+23664371	None:intergenic
TTGAAGCTAGAGGGTGCAAA+TGG	0.533573	2.4:-23664990	MS.gene95994:CDS
CTAACAGATTTCTCTTATGA+GGG	0.541945	2.4:-23664850	MS.gene95994:CDS
ATGCATCTCAGATAAGTGAA+TGG	0.564518	2.4:-23665269	MS.gene95994:CDS
CTTCAAACACTTTGAGAATG+GGG	0.565764	2.4:+23665007	None:intergenic
TTGATGGCTAGTTAAGCAAA+GGG	0.569586	2.4:+23665296	None:intergenic
GAACGAGCACGATCTTTGTC+TGG	0.572036	2.4:-23663941	MS.gene95994:CDS
CTGCAACTAAATGAGGTCAC+TGG	0.573768	2.4:-23664423	MS.gene95994:CDS
TTGTCTGGTGAAATTTGTGT+TGG	0.580431	2.4:-23663926	MS.gene95994:CDS
TTTCATTCCAATCATAGAAG+CGG	0.582039	2.4:-23665493	MS.gene95994:CDS
AGATGTCTTTACTTCTGCAA+TGG	0.584477	2.4:-23664019	MS.gene95994:CDS
TCAAAGTGTTTGAAGCTAGA+GGG	0.585679	2.4:-23664999	MS.gene95994:CDS
ATTGATGGCTAGTTAAGCAA+AGG	0.586648	2.4:+23665295	None:intergenic
TAAGTTAACCTTCCAAAGAG+TGG	0.599115	2.4:+23664447	None:intergenic
GAAAAGGTGAATTTGAAGGA+AGG	0.601206	2.4:+23665184	None:intergenic
GGAGACAAATGGATACGAGA+AGG	0.614255	2.4:+23665112	None:intergenic
TCTCAAACAAATTGATAACG+TGG	0.682010	2.4:-23664704	MS.gene95994:CDS
AAGCTTGACAGAAAGCAGTG+AGG	0.745455	2.4:+23663994	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTAAATTTCTTTGAACAAA+AGG	+	chr2.4:23665011-23665030	None:intergenic	15.0%
!!!	ATTTTTTTCAATTTGGATTT+TGG	+	chr2.4:23664776-23664795	None:intergenic	15.0%
!!	ATAAGCTAAAAACTAACTTA+TGG	+	chr2.4:23665145-23665164	None:intergenic	20.0%
!!	TTAAAAAACTTAGCATTTCT+GGG	-	chr2.4:23664234-23664253	MS.gene95994:intron	20.0%
!!!	ACTGAAATTATTGTTTTACA+AGG	-	chr2.4:23664640-23664659	MS.gene95994:intron	20.0%
!!!	GTTTTACTTAATGTAAGTTT+AGG	-	chr2.4:23664977-23664996	MS.gene95994:CDS	20.0%
!!!	TGCGAATATTTTTTTCAATT+TGG	+	chr2.4:23664783-23664802	None:intergenic	20.0%
!!!	TTCAAAGTATTTCAGTTTAA+AGG	-	chr2.4:23665344-23665363	MS.gene95994:CDS	20.0%
!	AGTGTTTATACTAGTAGATA+AGG	+	chr2.4:23665077-23665096	None:intergenic	25.0%
!	ATTGAAAACTTCTCAAGTAA+AGG	+	chr2.4:23664383-23664402	None:intergenic	25.0%
!	ATTTATCGCTGATATGAAAA+CGG	-	chr2.4:23665199-23665218	MS.gene95994:CDS	25.0%
!	CTTAAAAAACTTAGCATTTC+TGG	-	chr2.4:23664233-23664252	MS.gene95994:intron	25.0%
!	TAAAAAACTTAGCATTTCTG+GGG	-	chr2.4:23664235-23664254	MS.gene95994:intron	25.0%
!	TAAGAAGAAAAAGATGCAAA+AGG	-	chr2.4:23663932-23663951	MS.gene95994:CDS	25.0%
!	TACATGATTCATTTGATTGA+TGG	+	chr2.4:23664418-23664437	None:intergenic	25.0%
!	TGAAGATAAAAAGGATAGAA+TGG	-	chr2.4:23664562-23664581	MS.gene95994:intron	25.0%
!!	AGAAGTTTTCAATAGCAATA+TGG	-	chr2.4:23664390-23664409	MS.gene95994:CDS	25.0%
!!	AGTTATATCTTTTGAACAAG+TGG	+	chr2.4:23664282-23664301	None:intergenic	25.0%
!!	TGTTGTTGATGAAGATAAAA+AGG	-	chr2.4:23664553-23664572	MS.gene95994:intron	25.0%
!!!	AAAACAAGAGTGTTAAGAAT+AGG	+	chr2.4:23664951-23664970	None:intergenic	25.0%
!!!	AAACAAGAGTGTTAAGAATA+GGG	+	chr2.4:23664950-23664969	None:intergenic	25.0%
!!!	CAGAAATGCTAAGTTTTTTA+AGG	+	chr2.4:23664235-23664254	None:intergenic	25.0%
!!!	TGCTAAGTTTTTTAAGGTTT+TGG	+	chr2.4:23664229-23664248	None:intergenic	25.0%
!!!	TGTCTTTTAAGATACATGAA+TGG	-	chr2.4:23664669-23664688	MS.gene95994:intron	25.0%
	AAAAGATATGCAATAGTCGT+TGG	-	chr2.4:23664724-23664743	MS.gene95994:CDS	30.0%
	AAACTAATGTACTGTGTTGT+TGG	+	chr2.4:23664353-23664372	None:intergenic	30.0%
	AACTTATCTGCAACTAAATG+AGG	-	chr2.4:23664887-23664906	MS.gene95994:CDS	30.0%
	ACTATCATCAAACTTTAGTC+TGG	+	chr2.4:23663901-23663920	None:intergenic	30.0%
	ATCAAACTTTAGTCTGGTTA+TGG	+	chr2.4:23663895-23663914	None:intergenic	30.0%
	CTAACAGATTTCTCTTATGA+GGG	-	chr2.4:23664467-23664486	MS.gene95994:CDS	30.0%
	CTATGATTGGAATGAAAGAT+AGG	+	chr2.4:23663821-23663840	None:intergenic	30.0%
	GGATAAGATGAAGATATTGT+CGG	-	chr2.4:23665430-23665449	MS.gene95994:CDS	30.0%
	TTTCATTCCAATCATAGAAG+CGG	-	chr2.4:23663824-23663843	MS.gene95994:CDS	30.0%
!	ATATCTTTTGAACAAGTGGA+CGG	+	chr2.4:23664278-23664297	None:intergenic	30.0%
!	GCGATATGCAAATATTGATT+TGG	-	chr2.4:23665463-23665482	MS.gene95994:CDS	30.0%
!	GGATTTGCTTTATCTTAGAA+AGG	-	chr2.4:23664069-23664088	MS.gene95994:intron	30.0%
!!	AACAAGAGTGTTAAGAATAG+GGG	+	chr2.4:23664949-23664968	None:intergenic	30.0%
!!	AAGTTTGATGATAGTTTGCT+TGG	-	chr2.4:23663906-23663925	MS.gene95994:CDS	30.0%
!!	ACTTTACATGAAAGCATTCT+TGG	-	chr2.4:23663786-23663805	MS.gene95994:CDS	30.0%
!!	ATTTGGATTTTGGAAAAGAC+AGG	+	chr2.4:23664766-23664785	None:intergenic	30.0%
!!	TCTCAAACAAATTGATAACG+TGG	-	chr2.4:23664613-23664632	MS.gene95994:intron	30.0%
!!!	TTTAAGGTTTTGGAGACAAA+TGG	+	chr2.4:23664219-23664238	None:intergenic	30.0%
	AAACTATCCTAGAGAGCTTA+TGG	+	chr2.4:23665170-23665189	None:intergenic	35.0%
	AAGGATAGAATGGAAAAGCT+AGG	-	chr2.4:23664572-23664591	MS.gene95994:intron	35.0%
	ACTAGAGAATTGCATCGAAA+AGG	+	chr2.4:23664152-23664171	None:intergenic	35.0%
	AGATGTCTTTACTTCTGCAA+TGG	-	chr2.4:23665298-23665317	MS.gene95994:CDS	35.0%
	ATGCATCTCAGATAAGTGAA+TGG	-	chr2.4:23664048-23664067	MS.gene95994:intron	35.0%
	CATCGAAAAGGTGAATTTGA+AGG	+	chr2.4:23664140-23664159	None:intergenic	35.0%
	CATGACACGCAAAAATTTCA+GGG	-	chr2.4:23665257-23665276	MS.gene95994:CDS	35.0%
	CCTAACAGATTTCTCTTATG+AGG	-	chr2.4:23664466-23664485	MS.gene95994:CDS	35.0%
	CCTCATAAGAGAAATCTGTT+AGG	+	chr2.4:23664469-23664488	None:intergenic	35.0%
	CTTATTTCCATAAGCTCTCT+AGG	-	chr2.4:23665160-23665179	MS.gene95994:CDS	35.0%
	GAAAAGGTGAATTTGAAGGA+AGG	+	chr2.4:23664136-23664155	None:intergenic	35.0%
	TAAAATTTCCCTTGTCCTAC+AGG	-	chr2.4:23664812-23664831	MS.gene95994:CDS	35.0%
	TAAGTTAACCTTCCAAAGAG+TGG	+	chr2.4:23664873-23664892	None:intergenic	35.0%
	TCATGACACGCAAAAATTTC+AGG	-	chr2.4:23665256-23665275	MS.gene95994:CDS	35.0%
	TTAAGCAAAGGGAGAAACTT+AGG	+	chr2.4:23664013-23664032	None:intergenic	35.0%
	TTGCAGTTGTTAGAACACTT+TGG	+	chr2.4:23665518-23665537	None:intergenic	35.0%
	TTGTCTGGTGAAATTTGTGT+TGG	-	chr2.4:23665391-23665410	MS.gene95994:CDS	35.0%
!	AGCTTCAAACACTTTGAGAA+TGG	+	chr2.4:23664315-23664334	None:intergenic	35.0%
!	ATTGATGGCTAGTTAAGCAA+AGG	+	chr2.4:23664025-23664044	None:intergenic	35.0%
!	CTTCAAACACTTTGAGAATG+GGG	+	chr2.4:23664313-23664332	None:intergenic	35.0%
!	GCTTCAAACACTTTGAGAAT+GGG	+	chr2.4:23664314-23664333	None:intergenic	35.0%
!	TCAAAGTGTTTGAAGCTAGA+GGG	-	chr2.4:23664318-23664337	MS.gene95994:intron	35.0%
!	TCTGAGATGCATCATATTGA+TGG	+	chr2.4:23664040-23664059	None:intergenic	35.0%
!	TTGATGGCTAGTTAAGCAAA+GGG	+	chr2.4:23664024-23664043	None:intergenic	35.0%
!!	CAAGAAGATGGATTGAAGTT+TGG	-	chr2.4:23663865-23663884	MS.gene95994:CDS	35.0%
!!!	TGAAGATATTGTCGGCTTTT+TGG	-	chr2.4:23665438-23665457	MS.gene95994:CDS	35.0%
	CCTTAAAACCTGTAGGACAA+GGG	+	chr2.4:23664823-23664842	None:intergenic	40.0%
	CTAGCGTCTTATCAAGAAGA+TGG	-	chr2.4:23663853-23663872	MS.gene95994:CDS	40.0%
	TACCATGCCTTAAAACCTGT+AGG	+	chr2.4:23664830-23664849	None:intergenic	40.0%
!	CTCAAAGTGTTTGAAGCTAG+AGG	-	chr2.4:23664317-23664336	MS.gene95994:intron	40.0%
!!	GCGTCGACTATGAACTTTAA+TGG	+	chr2.4:23664447-23664466	None:intergenic	40.0%
	AAGCTTGACAGAAAGCAGTG+AGG	+	chr2.4:23665326-23665345	None:intergenic	45.0%
	CTGCAACTAAATGAGGTCAC+TGG	-	chr2.4:23664894-23664913	MS.gene95994:CDS	45.0%
	GCCTTAAAACCTGTAGGACA+AGG	+	chr2.4:23664824-23664843	None:intergenic	45.0%
	GGAGACAAATGGATACGAGA+AGG	+	chr2.4:23664208-23664227	None:intergenic	45.0%
	GTGCTTACCGCTTCTATGAT+TGG	+	chr2.4:23663834-23663853	None:intergenic	45.0%
	GTTGGCAAATGCTGAGATCT+TGG	-	chr2.4:23665409-23665428	MS.gene95994:CDS	45.0%
	TTCACCAATCTGCCACTCTT+TGG	-	chr2.4:23664858-23664877	MS.gene95994:CDS	45.0%
!	CCCTTGTCCTACAGGTTTTA+AGG	-	chr2.4:23664820-23664839	MS.gene95994:CDS	45.0%
!	TTGAAGCTAGAGGGTGCAAA+TGG	-	chr2.4:23664327-23664346	MS.gene95994:intron	45.0%
!!!	GTCCTACAGGTTTTAAGGCA+TGG	-	chr2.4:23664825-23664844	MS.gene95994:CDS	45.0%
	CCAATCTGCCACTCTTTGGA+AGG	-	chr2.4:23664862-23664881	MS.gene95994:CDS	50.0%
	CCTTCCAAAGAGTGGCAGAT+TGG	+	chr2.4:23664865-23664884	None:intergenic	50.0%
	GAACGAGCACGATCTTTGTC+TGG	-	chr2.4:23665376-23665395	MS.gene95994:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	23663777	23665562	23663777	ID=MS.gene95994
chr2.4	mRNA	23663777	23665562	23663777	ID=MS.gene95994.t1;Parent=MS.gene95994
chr2.4	exon	23664678	23665562	23664678	ID=MS.gene95994.t1.exon1;Parent=MS.gene95994.t1
chr2.4	CDS	23664678	23665562	23664678	ID=cds.MS.gene95994.t1;Parent=MS.gene95994.t1
chr2.4	exon	23664374	23664505	23664374	ID=MS.gene95994.t1.exon2;Parent=MS.gene95994.t1
chr2.4	CDS	23664374	23664505	23664374	ID=cds.MS.gene95994.t1;Parent=MS.gene95994.t1
chr2.4	exon	23663777	23664061	23663777	ID=MS.gene95994.t1.exon3;Parent=MS.gene95994.t1
chr2.4	CDS	23663777	23664061	23663777	ID=cds.MS.gene95994.t1;Parent=MS.gene95994.t1

Gene Sequence

>MS.gene95994

ATGATTAGCACTTTACATGAAAGCATTCTTGGTAAGCACATCCTATCTTTCATTCCAATCATAGAAGCGGTAAGCACTAGCGTCTTATCAAGAAGATGGATTGAAGTTTGGACATCCATAACCAGACTAAAGTTTGATGATAGTTTGCTTGGTTCTAAGAAGAAAAAGATGCAAAAGGAACAATTTGTGAATTTTGTTGAAAAAGTGCTTATTCACTTTACTAACTCAAGCATCCTAAGTTTCTCCCTTTGCTTAACTAGCCATCAATATGATGCATCTCAGATAAGTGAATGGATTTGCTTTATCTTAGAAAGGAGAGTTCAAAAGCTTCATATTCAGTATGCTGATAAAGTTTTCCTTCCTTCAAATTCACCTTTTCGATGCAATTCTCTAGTCGAATTGACGCTTCAAATGAGATGTGTTCTTAGCCTTCTCGTATCCATTTGTCTCCAAAACCTTAAAAAACTTAGCATTTCTGGGGTTAAATTAGTGAGCGATCCGTCCACTTGTTCAAAAGATATAACTCTCAATTTCCCCATTCTCAAAGTGTTTGAAGCTAGAGGGTGCAAATGGCCAACAACACAGTACATTAGTTTACAAGTACCTTTACTTGAGAAGTTTTCAATAGCAATATGGAACCATCAATCAAATGAATCATGTAAATATGCCATTAAAGTTCATAGTCGACGCCTAACAGATTTCTCTTATGAGGGTGATCTTCAACAAGACATTGTTCTATGTGATTCATCATCAATTCGCAATGCTTCTGTTGTGATTGTTGTTGATGAAGATAAAAAGGATAGAATGGAAAAGCTAGGATTTCAAGTACATAATCTTCTCAAACAAATTGATAACGTGGAACGACTGAAATTATTGTTTTACAAGGTATGCTTGTCTTTTAAGATACATGAATGGAAATAGTCTTGTATAACAAAAACTTTGTGATAAAAAGATATGCAATAGTCGTTGGATAGTAACAAGTTATGCCTGTCTTTTCCAAAATCCAAATTGAAAAAAATATTCGCAGAATTTGAATAAAATTTCCCTTGTCCTACAGGTTTTAAGGCATGGTAAAGATATTTTCACCAATCTGCCACTCTTTGGAAGGTTAACTTATCTGCAACTAAATGAGGTCACTGGTGAAGCTTTGTTGCAATTACTTCACAATTCCCCTATTCTTAACACTCTTGTTTTACTTAAGTTTTACTTAATGTAAGTTTAGGTACTCTTGCCTTTTGTTCAAAGAAATTTAATATCTTGTTAATGTGTTTAGTGTTGTTTAGATTGACATATTTGACCTTATCTACTAGTATAAACACTTGTGAGAATGTATGAGATAGATTATAGAAACAACTTATGACATGTCCATAAGTTAGTTTTTAGCTTATTTCCATAAGCTCTCTAGGATAGTTTATGAAAACAATTTATCGCTGATATGAAAACGGTTTAACTTAATTTATCTTTTATTATAGAAGTAACTCATGACACGCAAAAATTTCAGGGAGTAGCTGATTTAGACAAAGATGTCTTTACTTCTGCAATGGTGCCTCACTGCTTTCTGTCAAGCTTCAAAGTATTTCAGTTTAAAGGATTTAATGTGAACGAGCACGATCTTTGTCTGGTGAAATTTGTGTTGGCAAATGCTGAGATCTTGGATAAGATGAAGATATTGTCGGCTTTTTGGCTGCGATATGCAAATATTGATTTGGAAAAAGTTAATGAGCAGATACTCTCATTACCAAAGTGTTCTAACAACTGCAAGATAGAATTTTCTGATATCAGTTGA

Protein sequence

>MS.gene95994.t1

MISTLHESILGKHILSFIPIIEAVSTSVLSRRWIEVWTSITRLKFDDSLLGSKKKKMQKEQFVNFVEKVLIHFTNSSILSFSLCLTSHQYDASQISEWICFILERRVQKLHIQYADKVFLPSNSPFRCNSLVELTLQMRCVLSLLVSICLQNLKKLSISGVKLVSDPSTCSKDITLNFPILKVFEARGCKWPTTQYISLQVPLLEKFSIAIWNHQSNESCKYAIKVHSRRLTDFSYEGDLQQDIVLCDSSSIRNASVVIVVDEDKKDRMEKLGFQVHNLLKQIDNVERLKLLFYKVLRHGKDIFTNLPLFGRLTYLQLNEVTGEALLQLLHNSPILNTLGVADLDKDVFTSAMVPHCFLSSFKVFQFKGFNVNEHDLCLVKFVLANAEILDKMKILSAFWLRYANIDLEKVNEQILSLPKCSNNCKIEFSDIS