AlfalfaGEDB | MS.gene95995

M. sativa cultivar XinJiangDaYe / MS.gene95995

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95995.t1	XP_013464451.1	79.5	302	62	0	19	320	6	307	2.30E-130	475.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95995.t1	Q9LUC4	32.8	259	165	6	29	284	29	281	2.2e-25	117.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95995.t1	A0A072VA50	79.5	302	62	0	19	320	6	307	1.6e-130	475.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene95995	MS.gene25298	PPI
MS.gene95995	MS.gene25448	PPI
MS.gene63259	MS.gene95995	PPI
MS.gene63213	MS.gene95995	PPI
MS.gene34824	MS.gene95995	PPI
MS.gene64993	MS.gene95995	PPI
MS.gene25411	MS.gene95995	PPI
MS.gene72959	MS.gene95995	PPI
MS.gene25414	MS.gene95995	PPI
MS.gene34739	MS.gene95995	PPI
MS.gene95995	MS.gene34737	PPI
MS.gene34731	MS.gene95995	PPI
MS.gene062282	MS.gene95995	PPI
MS.gene25386	MS.gene95995	PPI
MS.gene065516	MS.gene95995	PPI
MS.gene25352	MS.gene95995	PPI
MS.gene34704	MS.gene95995	PPI
MS.gene61631	MS.gene95995	PPI
MS.gene48654	MS.gene95995	PPI
MS.gene067702	MS.gene95995	PPI
MS.gene95995	MS.gene017193	PPI
MS.gene63212	MS.gene95995	PPI
MS.gene36066	MS.gene95995	PPI
MS.gene66626	MS.gene95995	PPI
MS.gene95995	MS.gene63221	PPI
MS.gene64984	MS.gene95995	PPI
MS.gene72958	MS.gene95995	PPI
MS.gene95995	MS.gene63222	PPI
MS.gene63260	MS.gene95995	PPI
MS.gene038427	MS.gene95995	PPI
MS.gene25299	MS.gene95995	PPI
MS.gene95995	MS.gene34733	PPI
MS.gene95995	MS.gene34825	PPI
MS.gene64992	MS.gene95995	PPI
MS.gene95995	MS.gene99980	PPI
MS.gene63210	MS.gene95995	PPI
MS.gene72960	MS.gene95995	PPI
MS.gene95995	MS.gene043122	PPI
MS.gene95995	MS.gene63256	PPI
MS.gene95995	MS.gene34740	PPI
MS.gene053312	MS.gene95995	PPI
MS.gene95995	MS.gene032295	PPI
MS.gene95995	MS.gene92095	PPI
MS.gene25344	MS.gene95995	PPI
MS.gene061286	MS.gene95995	PPI
MS.gene065234	MS.gene95995	PPI
MS.gene34706	MS.gene95995	PPI
MS.gene34753	MS.gene95995	PPI
MS.gene95995	MS.gene007875	PPI
MS.gene34216	MS.gene95995	PPI
MS.gene72954	MS.gene95995	PPI
MS.gene060770	MS.gene95995	PPI
MS.gene055882	MS.gene95995	PPI
MS.gene63224	MS.gene95995	PPI
MS.gene95995	MS.gene34736	PPI
MS.gene99983	MS.gene95995	PPI
MS.gene95995	MS.gene34730	PPI
MS.gene95995	MS.gene25418	PPI
MS.gene49513	MS.gene95995	PPI
MS.gene69850	MS.gene95995	PPI
MS.gene72957	MS.gene95995	PPI
MS.gene63257	MS.gene95995	PPI
MS.gene060769	MS.gene95995	PPI
MS.gene055883	MS.gene95995	PPI
MS.gene63214	MS.gene95995	PPI
MS.gene25296	MS.gene95995	PPI
MS.gene63217	MS.gene95995	PPI
MS.gene95995	MS.gene25451	PPI
MS.gene032296	MS.gene95995	PPI
MS.gene25450	MS.gene95995	PPI
MS.gene36067	MS.gene95995	PPI
MS.gene25297	MS.gene95995	PPI
MS.gene63177	MS.gene95995	PPI
MS.gene25449	MS.gene95995	PPI
MS.gene72952	MS.gene95995	PPI
MS.gene95995	MS.gene34766	PPI
MS.gene060771	MS.gene95995	PPI
MS.gene34801	MS.gene95995	PPI
MS.gene72955	MS.gene95995	PPI
MS.gene63104	MS.gene95995	PPI
MS.gene95995	MS.gene72956	PPI
MS.gene053313	MS.gene95995	PPI
MS.gene99982	MS.gene95995	PPI
MS.gene34707	MS.gene95995	PPI
MS.gene72962	MS.gene95995	PPI
MS.gene63218	MS.gene95995	PPI
MS.gene25396	MS.gene95995	PPI
MS.gene93027	MS.gene95995	PPI
MS.gene95995	MS.gene72961	PPI
MS.gene63111	MS.gene95995	PPI
MS.gene34215	MS.gene95995	PPI
MS.gene073176	MS.gene95995	PPI
MS.gene34804	MS.gene95995	PPI
MS.gene95995	MS.gene63105	PPI
MS.gene25347	MS.gene95995	PPI
MS.gene48656	MS.gene95995	PPI
MS.gene64989	MS.gene95995	PPI
MS.gene63223	MS.gene95995	PPI
MS.gene062526	MS.gene95995	PPI
MS.gene95995	MS.gene007876	PPI
MS.gene055877	MS.gene95995	PPI
MS.gene34738	MS.gene95995	PPI
MS.gene95995	MS.gene34802	PPI
MS.gene72963	MS.gene95995	PPI
MS.gene25353	MS.gene95995	PPI
MS.gene95995	MS.gene36065	PPI
MS.gene056517	MS.gene95995	PPI
MS.gene34735	MS.gene95995	PPI
MS.gene25415	MS.gene95995	PPI
MS.gene34703	MS.gene95995	PPI
MS.gene95995	MS.gene95710	PPI
MS.gene63095	MS.gene95995	PPI
MS.gene055880	MS.gene95995	PPI
MS.gene043123	MS.gene95995	PPI
MS.gene25412	MS.gene95995	PPI
MS.gene25410	MS.gene95995	PPI
MS.gene95995	MS.gene64988	PPI
MS.gene25348	MS.gene95995	PPI
MS.gene63113	MS.gene95995	PPI
MS.gene63185	MS.gene95995	PPI
MS.gene63094	MS.gene95995	PPI
MS.gene053311	MS.gene95995	PPI
MS.gene062537	MS.gene95995	PPI
MS.gene63112	MS.gene95995	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95995.t1	MTR_2g072530	79.208	303	63	0	19	321	6	308	7.89e-173	487
MS.gene95995.t1	MTR_2g072550	82.400	125	22	0	197	321	31	155	1.94e-68	214
MS.gene95995.t1	MTR_2g082800	40.590	271	150	7	27	295	28	289	9.51e-49	168
MS.gene95995.t1	MTR_2g082850	36.913	298	170	9	1	290	1	288	5.90e-45	157
MS.gene95995.t1	MTR_7g094030	33.537	328	199	9	1	319	1	318	1.21e-43	156
MS.gene95995.t1	MTR_2g082880	33.438	317	193	8	18	326	2	308	2.05e-41	149
MS.gene95995.t1	MTR_2g082780	39.604	202	118	4	29	229	15	213	2.30e-38	135
MS.gene95995.t1	MTR_2g072730	35.333	300	176	11	27	312	17	312	1.06e-33	129
MS.gene95995.t1	MTR_2g072720	35.065	308	172	11	27	316	40	337	1.75e-33	129
MS.gene95995.t1	MTR_1g115250	32.364	275	152	8	27	290	33	284	2.07e-31	122
MS.gene95995.t1	MTR_1g115245	31.579	285	169	8	1	274	1	270	4.98e-30	116
MS.gene95995.t1	MTR_4g032845	30.065	306	190	10	27	316	4	301	1.48e-28	115
MS.gene95995.t1	MTR_2g067210	29.697	330	196	11	10	308	26	350	5.18e-24	102
MS.gene95995.t1	MTR_5g007130	28.395	324	193	9	17	316	6	314	3.77e-22	96.7
MS.gene95995.t1	MTR_5g063450	30.128	312	179	10	28	330	29	310	2.14e-20	90.9
MS.gene95995.t1	MTR_6g022350	26.331	357	189	11	24	316	5	351	6.58e-19	87.8
MS.gene95995.t1	MTR_5g005700	29.582	311	165	13	16	279	17	320	1.14e-18	87.0
MS.gene95995.t1	MTR_5g005690	25.166	302	181	8	18	282	12	305	3.10e-18	85.5
MS.gene95995.t1	MTR_7g023310	24.060	266	187	7	24	282	24	281	2.68e-16	79.7
MS.gene95995.t1	MTR_1g025870	31.522	276	164	13	57	316	116	382	4.20e-16	79.3
MS.gene95995.t1	MTR_3g018680	30.876	217	125	8	31	234	27	231	4.22e-15	75.9
MS.gene95995.t1	MTR_4g081030	26.692	266	162	9	28	282	19	262	7.37e-15	75.1
MS.gene95995.t1	MTR_8g006910	30.702	228	127	9	31	245	57	266	9.70e-15	75.1
MS.gene95995.t1	MTR_5g007090	30.516	213	106	8	24	197	28	237	2.79e-14	73.9
MS.gene95995.t1	MTR_5g084070	31.159	138	86	3	28	164	15	144	4.27e-14	73.2
MS.gene95995.t1	MTR_3g048290	26.147	218	145	7	27	235	24	234	9.16e-14	72.4
MS.gene95995.t1	MTR_2g082860	42.683	82	45	2	13	93	13	93	7.05e-13	65.1
MS.gene95995.t1	MTR_4g080590	26.000	300	205	10	22	316	19	306	8.28e-13	68.9
MS.gene95995.t1	MTR_4g080590	25.503	298	209	9	22	316	19	306	1.15e-12	68.9
MS.gene95995.t1	MTR_7g026500	33.143	175	97	5	28	188	16	184	1.71e-12	68.2
MS.gene95995.t1	MTR_7g032380	30.952	168	95	7	28	184	13	170	1.87e-12	67.8
MS.gene95995.t1	MTR_5g058100	31.980	197	123	7	19	211	2	191	3.27e-12	67.0
MS.gene95995.t1	MTR_0049s0110	31.746	189	112	9	27	203	9	192	5.01e-12	65.9
MS.gene95995.t1	MTR_0049s0110	31.746	189	112	9	27	203	9	192	9.04e-12	65.9
MS.gene95995.t1	MTR_0049s0110	31.746	189	112	9	27	203	9	192	1.07e-11	65.5
MS.gene95995.t1	MTR_0049s0110	31.746	189	112	9	27	203	9	192	1.07e-11	65.5
MS.gene95995.t1	MTR_3g105060	34.286	140	79	5	28	161	16	148	1.16e-11	65.5
MS.gene95995.t1	MTR_4g098590	28.643	199	121	7	28	211	1	193	1.37e-11	65.1
MS.gene95995.t1	MTR_2g007500	28.033	239	133	10	28	252	16	229	2.08e-11	64.3
MS.gene95995.t1	MTR_7g056430	30.539	167	96	6	28	191	11	160	2.93e-11	65.1
MS.gene95995.t1	MTR_7g056420	30.539	167	96	6	28	191	11	160	3.06e-11	65.1
MS.gene95995.t1	MTR_7g075820	29.801	151	88	4	28	161	8	157	6.02e-11	63.5
MS.gene95995.t1	MTR_3g107100	30.769	143	84	5	28	162	13	148	6.72e-11	63.2
MS.gene95995.t1	MTR_6g022330	48.529	68	28	2	15	75	1	68	7.31e-11	63.5
MS.gene95995.t1	MTR_3g107160	32.192	146	81	5	25	159	9	147	7.52e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95995.t1	AT3G14710	33.333	255	161	6	29	280	29	277	2.09e-27	111
MS.gene95995.t1	AT3G03030	27.517	298	196	10	27	313	3	291	1.60e-18	85.5
MS.gene95995.t1	AT3G03030	27.425	299	197	10	27	314	3	292	4.73e-18	85.1
MS.gene95995.t1	AT4G10400	32.353	170	106	3	28	193	4	168	8.79e-17	80.9
MS.gene95995.t1	AT4G10400	32.353	170	106	3	28	193	4	168	8.79e-17	80.9
MS.gene95995.t1	AT4G10400	32.353	170	106	3	28	193	4	168	8.79e-17	80.9
MS.gene95995.t1	AT4G14096	31.818	220	143	6	23	239	5	220	1.39e-16	80.9
MS.gene95995.t1	AT4G14096	31.818	220	143	6	23	239	5	220	1.39e-16	80.9
MS.gene95995.t1	AT1G48400	32.000	200	124	7	25	217	9	203	1.64e-16	80.5
MS.gene95995.t1	AT4G09920	31.765	170	107	3	28	193	4	168	1.85e-16	79.3
MS.gene95995.t1	AT4G09920	31.765	170	107	3	28	193	4	168	1.85e-16	79.3
MS.gene95995.t1	AT4G09920	31.765	170	107	3	28	193	4	168	1.93e-16	79.0
MS.gene95995.t1	AT4G09920	31.765	170	107	3	28	193	4	168	1.93e-16	79.0
MS.gene95995.t1	AT4G09920	31.765	170	107	3	28	193	4	168	1.93e-16	79.0
MS.gene95995.t1	AT1G48400	32.000	200	124	7	25	217	9	203	2.04e-16	80.1
MS.gene95995.t1	AT4G14103	37.762	143	84	4	23	162	5	145	2.52e-16	79.3
MS.gene95995.t1	AT4G14103	37.762	143	84	4	23	162	5	145	2.52e-16	79.3
MS.gene95995.t1	AT4G14103	37.762	143	84	4	23	162	5	145	3.09e-16	79.7
MS.gene95995.t1	AT3G58940	31.250	208	117	10	28	216	4	204	5.21e-16	79.3
MS.gene95995.t1	AT3G58940	31.250	208	117	10	28	216	4	204	5.21e-16	79.3
MS.gene95995.t1	AT3G59200	34.545	165	100	4	25	183	6	168	6.09e-16	78.2
MS.gene95995.t1	AT3G59200	34.545	165	100	4	25	183	6	168	1.16e-15	78.2
MS.gene95995.t1	AT3G03040	28.358	201	128	7	27	216	3	198	2.82e-15	76.6
MS.gene95995.t1	AT2G29910	28.405	257	160	9	22	265	1	246	3.32e-15	75.9
MS.gene95995.t1	AT2G29910	28.405	257	160	9	22	265	1	246	3.32e-15	75.9
MS.gene95995.t1	AT3G58930	25.228	329	197	12	28	321	4	318	4.39e-15	76.3
MS.gene95995.t1	AT3G58930	25.228	329	197	12	28	321	4	318	4.39e-15	76.3
MS.gene95995.t1	AT3G58930	25.228	329	197	12	28	321	4	318	4.39e-15	76.3
MS.gene95995.t1	AT2G29910	28.405	257	160	9	22	265	25	270	4.64e-15	75.9
MS.gene95995.t1	AT2G29910	28.405	257	160	9	22	265	1	246	4.71e-15	75.5
MS.gene95995.t1	AT2G29910	28.405	257	160	9	22	265	1	246	4.71e-15	75.5
MS.gene95995.t1	AT2G29910	28.405	257	160	9	22	265	1	246	4.71e-15	75.5
MS.gene95995.t1	AT2G29910	28.405	257	160	9	22	265	1	246	4.71e-15	75.5
MS.gene95995.t1	AT3G59230	27.237	257	153	11	17	263	3	235	4.56e-14	73.2
MS.gene95995.t1	AT3G42770	34.706	170	99	6	28	186	1	169	1.08e-13	72.0
MS.gene95995.t1	AT4G26340	33.529	170	103	3	28	193	4	167	1.32e-13	71.6
MS.gene95995.t1	AT5G56690	28.505	214	143	6	28	235	4	213	1.40e-13	71.6
MS.gene95995.t1	AT3G58880	30.201	149	96	3	27	169	4	150	3.04e-13	70.5
MS.gene95995.t1	AT1G06630	28.378	222	144	8	23	234	9	225	3.11e-13	69.7
MS.gene95995.t1	AT3G49150	30.769	182	119	5	24	200	14	193	3.15e-13	70.5
MS.gene95995.t1	AT1G06630	28.378	222	144	8	23	234	9	225	4.38e-13	69.7
MS.gene95995.t1	AT1G06630	28.378	222	144	8	23	234	9	225	4.38e-13	69.7
MS.gene95995.t1	AT3G59190	30.286	175	106	7	23	186	9	178	5.24e-13	69.7
MS.gene95995.t1	AT4G26350	31.395	172	92	6	27	186	3	160	6.27e-13	69.7
MS.gene95995.t1	AT3G26922	26.901	171	118	2	28	196	16	181	6.60e-13	68.9
MS.gene95995.t1	AT3G26922	26.901	171	118	2	28	196	16	181	6.60e-13	68.9
MS.gene95995.t1	AT3G59190	30.286	175	106	7	23	186	9	178	6.66e-13	69.7
MS.gene95995.t1	AT1G06630	28.378	222	144	8	23	234	9	225	6.84e-13	69.7
MS.gene95995.t1	AT1G06630	28.378	222	144	8	23	234	9	225	7.34e-13	69.3
MS.gene95995.t1	AT1G06630	28.378	222	144	8	23	234	9	225	7.34e-13	69.3
MS.gene95995.t1	AT1G06630	28.378	222	144	8	23	234	9	225	7.34e-13	69.3
MS.gene95995.t1	AT1G06630	28.378	222	144	8	23	234	9	225	7.34e-13	69.3
MS.gene95995.t1	AT4G27050	32.450	151	82	6	27	161	5	151	7.42e-13	69.3
MS.gene95995.t1	AT4G27050	32.450	151	82	6	27	161	5	151	7.42e-13	69.3
MS.gene95995.t1	AT4G27050	32.450	151	82	6	27	161	5	151	7.42e-13	69.3
MS.gene95995.t1	AT4G27050	32.450	151	82	6	27	161	5	151	7.42e-13	69.3
MS.gene95995.t1	AT4G27050	32.450	151	82	6	27	161	5	151	7.42e-13	69.3
MS.gene95995.t1	AT3G58900	38.168	131	69	6	27	148	3	130	7.50e-13	68.6
MS.gene95995.t1	AT1G06630	28.378	222	144	8	23	234	18	234	7.69e-13	69.3
MS.gene95995.t1	AT5G41630	30.220	182	108	7	23	188	9	187	1.18e-12	68.9
MS.gene95995.t1	AT3G58900	38.168	131	69	6	27	148	3	130	1.22e-12	68.2
MS.gene95995.t1	AT3G58900	38.168	131	69	6	27	148	3	130	1.22e-12	68.2
MS.gene95995.t1	AT3G58900	38.168	131	69	6	27	148	3	130	1.53e-12	68.6
MS.gene95995.t1	AT3G59170	29.279	222	123	8	27	216	8	227	1.57e-12	68.6
MS.gene95995.t1	AT5G56400	31.953	169	107	5	28	193	35	198	1.84e-12	68.2
MS.gene95995.t1	AT3G58860	23.810	294	202	10	27	312	8	287	3.05e-12	67.8
MS.gene95995.t1	AT4G10410	33.566	143	89	2	44	185	5	142	3.60e-12	66.2
MS.gene95995.t1	AT1G16930	31.522	184	109	6	19	193	4	179	6.05e-12	66.6
MS.gene95995.t1	AT1G16930	31.522	184	109	6	19	193	4	179	6.05e-12	66.6
MS.gene95995.t1	AT1G16930	31.522	184	109	6	19	193	4	179	6.05e-12	66.6
MS.gene95995.t1	AT5G56380	29.091	220	132	8	28	233	4	213	7.31e-12	66.2
MS.gene95995.t1	AT5G56380	29.091	220	132	8	28	233	4	213	7.31e-12	66.2
MS.gene95995.t1	AT1G58310	33.333	186	97	7	25	187	7	188	1.08e-11	65.9
MS.gene95995.t1	AT2G04230	32.000	175	105	7	28	193	15	184	1.57e-11	65.5
MS.gene95995.t1	AT3G60040	31.694	183	107	7	21	186	225	406	1.60e-11	65.9
MS.gene95995.t1	AT3G60040	31.694	183	107	7	21	186	8	189	1.60e-11	65.5
MS.gene95995.t1	AT3G60040	31.694	183	107	7	21	186	227	408	1.68e-11	65.5
MS.gene95995.t1	AT3G60040	31.694	183	107	7	21	186	228	409	1.80e-11	65.9
MS.gene95995.t1	AT3G60040	31.694	183	107	7	21	186	227	408	1.86e-11	65.5
MS.gene95995.t1	AT1G64540	30.198	202	114	8	25	219	4	185	3.49e-11	64.3
MS.gene95995.t1	AT1G64540	30.198	202	114	8	25	219	4	185	3.49e-11	64.3
MS.gene95995.t1	AT4G13960	29.775	178	113	5	28	196	4	178	3.53e-11	64.3
MS.gene95995.t1	AT4G13960	29.775	178	113	5	28	196	4	178	3.53e-11	64.3
MS.gene95995.t1	AT3G49040	30.455	220	124	8	28	234	19	222	4.01e-11	63.5
MS.gene95995.t1	AT3G59160	28.713	202	116	6	23	197	10	210	4.30e-11	63.9
MS.gene95995.t1	AT5G41830	30.811	185	105	9	23	188	10	190	4.51e-11	64.3
MS.gene95995.t1	AT5G56420	27.841	176	117	4	22	193	2	171	8.97e-11	63.2
MS.gene95995.t1	AT5G56420	27.841	176	117	4	22	193	2	171	8.97e-11	63.2
MS.gene95995.t1	AT5G56420	27.841	176	117	4	22	193	2	171	8.97e-11	63.2

Find 43 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGATTACAAGTACCTTTAC+TGG	0.249535	2.4:-23659549	MS.gene95995:CDS
ACTTTACATGAAAGCATTCT+TGG	0.257982	2.4:-23660127	MS.gene95995:CDS
ATCAAACTTTAGTCTGGTTA+TGG	0.303311	2.4:+23660024	None:intergenic
GCTTCAAACACTTTGAGAAT+TGG	0.307798	2.4:+23659605	None:intergenic
CTTCAAACACTTTGAGAATT+GGG	0.314453	2.4:+23659606	None:intergenic
TGTTGTTGATGAAGATAAAA+AGG	0.327183	2.4:-23659363	MS.gene95995:CDS
CAAGAAGATGGATTGAAGTT+TGG	0.338077	2.4:-23660051	MS.gene95995:CDS
GCGTGAACAATAAACTTTCA+TGG	0.353000	2.4:+23659472	None:intergenic
CCTACTGTTGTTTCCCCTGA+TGG	0.366863	2.4:+23660169	None:intergenic
CCTAACGGATTTCTCTTATG+AGG	0.409012	2.4:-23659450	MS.gene95995:CDS
TTAAGCAAAGAGAGAAACTT+TGG	0.410637	2.4:+23659906	None:intergenic
ACTATCATCAAACTTTAGTC+TGG	0.424654	2.4:+23660018	None:intergenic
TCATCAACAACAATCACAAT+AGG	0.450854	2.4:+23659374	None:intergenic
GAAACAAAACACCTTCCATC+AGG	0.451496	2.4:-23660184	MS.gene95995:CDS
TTATGAGGGTGATCTTGAAC+AGG	0.451966	2.4:-23659435	MS.gene95995:CDS
GGATTTGCTTTATCTTAGAA+AGG	0.458843	2.4:-23659847	MS.gene95995:CDS
AAGTTTGATGATAGTTTGCT+TGG	0.483093	2.4:-23660010	MS.gene95995:CDS
TTTAAGACAAATCGATGAAG+TGG	0.500710	2.4:-23659303	MS.gene95995:CDS
GTGCTAACCGCGTCTACGGT+TGG	0.508651	2.4:+23660085	None:intergenic
ATGGAAAACTTCTCCAGTAA+AGG	0.510366	2.4:+23659536	None:intergenic
CTAGCGTCATATCAAGAAGA+TGG	0.510423	2.4:-23660063	MS.gene95995:CDS
ATTGCAGCTTAAAAGTGAAT+CGG	0.512067	2.4:+23659775	None:intergenic
AGTTTATTGTTCACGCCTAA+CGG	0.517642	2.4:-23659465	MS.gene95995:CDS
CTGTTGTTTCCCCTGATGGA+AGG	0.521711	2.4:+23660173	None:intergenic
GCGTCTACGGTTGGAATGAA+AGG	0.525082	2.4:+23660094	None:intergenic
ATGCATCTCGGATAAGTGAA+TGG	0.533819	2.4:-23659868	MS.gene95995:CDS
TACACGATTCATTTGAATGA+CGG	0.535085	2.4:+23659501	None:intergenic
ATGACGGTTCCATATTGCTA+TGG	0.536292	2.4:+23659517	None:intergenic
CTCAAAGTGTTTGAAGCTAG+AGG	0.539243	2.4:-23659599	MS.gene95995:CDS
AATCTAATGTTGTGTGTCGT+TGG	0.561856	2.4:+23659566	None:intergenic
CTAACGGATTTCTCTTATGA+GGG	0.562352	2.4:-23659449	MS.gene95995:CDS
GCTAGTGCTAACCGCGTCTA+CGG	0.575047	2.4:+23660081	None:intergenic
TTAAAAGTGAATCGGAAGGA+AGG	0.580749	2.4:+23659783	None:intergenic
CTACGGTTGGAATGAAAGGT+AGG	0.585426	2.4:+23660098	None:intergenic
AAACAAAACACCTTCCATCA+GGG	0.590148	2.4:-23660183	MS.gene95995:CDS
GTGATCAATATCATGCATCT+CGG	0.592830	2.4:-23659880	MS.gene95995:CDS
CCTCATAAGAGAAATCCGTT+AGG	0.618999	2.4:+23659450	None:intergenic
AACAAAACACCTTCCATCAG+GGG	0.630441	2.4:-23660182	MS.gene95995:CDS
CAGCTTAAAAGTGAATCGGA+AGG	0.637096	2.4:+23659779	None:intergenic
CCATCAGGGGAAACAACAGT+AGG	0.646826	2.4:-23660169	MS.gene95995:CDS
TTTCATTCCAACCGTAGACG+CGG	0.662255	2.4:-23660092	MS.gene95995:CDS
TTGAAGCTAGAGGATGTGAA+TGG	0.667284	2.4:-23659589	MS.gene95995:CDS
GGATTCGAACAATGCAACGA+CGG	0.667738	2.4:-23660212	MS.gene95995:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTTATATCTTTTGAATAAT+TGG	+	chr2.4:23659821-23659840	None:intergenic	15.0%
!!!	ACTGAAATTATTGTTTTACA+AGG	-	chr2.4:23660179-23660198	MS.gene95995:CDS	20.0%
!!!	TATATCTTTTGAATAATTGG+AGG	+	chr2.4:23659818-23659837	None:intergenic	20.0%
!	TAAGAAGAAAAAGATGCAAA+AGG	-	chr2.4:23659471-23659490	MS.gene95995:CDS	25.0%
!	TTCAAAAACTTAGCATTTCT+GGG	-	chr2.4:23659773-23659792	MS.gene95995:CDS	25.0%
!!	TGTTGTTGATGAAGATAAAA+AGG	-	chr2.4:23660092-23660111	MS.gene95995:CDS	25.0%
!!	TTTCTTTTGTGATAGTTCAA+TGG	-	chr2.4:23660208-23660227	MS.gene95995:CDS	25.0%
	ACTATCATCAAACTTTAGTC+TGG	+	chr2.4:23659440-23659459	None:intergenic	30.0%
	ATCAAACTTTAGTCTGGTTA+TGG	+	chr2.4:23659434-23659453	None:intergenic	30.0%
	ATTGCAGCTTAAAAGTGAAT+CGG	+	chr2.4:23659683-23659702	None:intergenic	30.0%
	CTTCAAAAACTTAGCATTTC+TGG	-	chr2.4:23659772-23659791	MS.gene95995:CDS	30.0%
	TACACGATTCATTTGAATGA+CGG	+	chr2.4:23659957-23659976	None:intergenic	30.0%
	TAGATTACAAGTACCTTTAC+TGG	-	chr2.4:23659906-23659925	MS.gene95995:CDS	30.0%
	TCATCAACAACAATCACAAT+AGG	+	chr2.4:23660084-23660103	None:intergenic	30.0%
	TGAAGATAAAAAGGACAGAA+CGG	-	chr2.4:23660101-23660120	MS.gene95995:CDS	30.0%
	TTAAGCAAAGAGAGAAACTT+TGG	+	chr2.4:23659552-23659571	None:intergenic	30.0%
!	AGAAGTTTTCCATAGCAATA+TGG	-	chr2.4:23659929-23659948	MS.gene95995:CDS	30.0%
!	CAGAAATGCTAAGTTTTTGA+AGG	+	chr2.4:23659774-23659793	None:intergenic	30.0%
!	CTTCAAACACTTTGAGAATT+GGG	+	chr2.4:23659852-23659871	None:intergenic	30.0%
!	GGATTTGCTTTATCTTAGAA+AGG	-	chr2.4:23659608-23659627	MS.gene95995:CDS	30.0%
!	TTTAAGACAAATCGATGAAG+TGG	-	chr2.4:23660152-23660171	MS.gene95995:CDS	30.0%
!!	AAGTTTGATGATAGTTTGCT+TGG	-	chr2.4:23659445-23659464	MS.gene95995:CDS	30.0%
!!	ACTTTACATGAAAGCATTCT+TGG	-	chr2.4:23659328-23659347	MS.gene95995:CDS	30.0%
!!	TGCTAAGTTTTTGAAGGTTT+TGG	+	chr2.4:23659768-23659787	None:intergenic	30.0%
!!	TTTTGGAGACAAACAGATAT+GGG	+	chr2.4:23659751-23659770	None:intergenic	30.0%
	AAACAAAACACCTTCCATCA+GGG	-	chr2.4:23659272-23659291	MS.gene95995:CDS	35.0%
	AATCTAATGTTGTGTGTCGT+TGG	+	chr2.4:23659892-23659911	None:intergenic	35.0%
	AGTTTATTGTTCACGCCTAA+CGG	-	chr2.4:23659990-23660009	MS.gene95995:CDS	35.0%
	ATGGAAAACTTCTCCAGTAA+AGG	+	chr2.4:23659922-23659941	None:intergenic	35.0%
	GCGTGAACAATAAACTTTCA+TGG	+	chr2.4:23659986-23660005	None:intergenic	35.0%
	GTGATCAATATCATGCATCT+CGG	-	chr2.4:23659575-23659594	MS.gene95995:CDS	35.0%
	TTAAAAGTGAATCGGAAGGA+AGG	+	chr2.4:23659675-23659694	None:intergenic	35.0%
!	GCTTCAAACACTTTGAGAAT+TGG	+	chr2.4:23659853-23659872	None:intergenic	35.0%
!!	CAAGAAGATGGATTGAAGTT+TGG	-	chr2.4:23659404-23659423	MS.gene95995:CDS	35.0%
!!	CTAACGGATTTCTCTTATGA+GGG	-	chr2.4:23660006-23660025	MS.gene95995:CDS	35.0%
!!!	GTTTTGGAGACAAACAGATA+TGG	+	chr2.4:23659752-23659771	None:intergenic	35.0%
	AACAAAACACCTTCCATCAG+GGG	-	chr2.4:23659273-23659292	MS.gene95995:CDS	40.0%
	ATGACGGTTCCATATTGCTA+TGG	+	chr2.4:23659941-23659960	None:intergenic	40.0%
	ATGCATCTCGGATAAGTGAA+TGG	-	chr2.4:23659587-23659606	MS.gene95995:CDS	40.0%
	CAGCTTAAAAGTGAATCGGA+AGG	+	chr2.4:23659679-23659698	None:intergenic	40.0%
	CCTCATAAGAGAAATCCGTT+AGG	+	chr2.4:23660008-23660027	None:intergenic	40.0%
	CTAGCGTCATATCAAGAAGA+TGG	-	chr2.4:23659392-23659411	MS.gene95995:CDS	40.0%
	GAAACAAAACACCTTCCATC+AGG	-	chr2.4:23659271-23659290	MS.gene95995:CDS	40.0%
	TTATGAGGGTGATCTTGAAC+AGG	-	chr2.4:23660020-23660039	MS.gene95995:CDS	40.0%
	TTGAAGCTAGAGGATGTGAA+TGG	-	chr2.4:23659866-23659885	MS.gene95995:CDS	40.0%
!	CTCAAAGTGTTTGAAGCTAG+AGG	-	chr2.4:23659856-23659875	MS.gene95995:CDS	40.0%
!!	CCTAACGGATTTCTCTTATG+AGG	-	chr2.4:23660005-23660024	MS.gene95995:CDS	40.0%
	CTACGGTTGGAATGAAAGGT+AGG	+	chr2.4:23659360-23659379	None:intergenic	45.0%
	GGATTCGAACAATGCAACGA+CGG	-	chr2.4:23659243-23659262	MS.gene95995:CDS	45.0%
	TTTCATTCCAACCGTAGACG+CGG	-	chr2.4:23659363-23659382	MS.gene95995:CDS	45.0%
	CCATCAGGGGAAACAACAGT+AGG	-	chr2.4:23659286-23659305	MS.gene95995:CDS	50.0%
	GCGTCTACGGTTGGAATGAA+AGG	+	chr2.4:23659364-23659383	None:intergenic	50.0%
!!	CCTACTGTTGTTTCCCCTGA+TGG	+	chr2.4:23659289-23659308	None:intergenic	50.0%
!!	CTGTTGTTTCCCCTGATGGA+AGG	+	chr2.4:23659285-23659304	None:intergenic	50.0%
	GCTAGTGCTAACCGCGTCTA+CGG	+	chr2.4:23659377-23659396	None:intergenic	55.0%
	GTGCTAACCGCGTCTACGGT+TGG	+	chr2.4:23659373-23659392	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	23659241	23660236	23659241	ID=MS.gene95995
chr2.4	mRNA	23659241	23660236	23659241	ID=MS.gene95995.t1;Parent=MS.gene95995
chr2.4	exon	23659241	23660236	23659241	ID=MS.gene95995.t1.exon1;Parent=MS.gene95995.t1
chr2.4	CDS	23659241	23660236	23659241	ID=cds.MS.gene95995.t1;Parent=MS.gene95995.t1

Gene Sequence

>MS.gene95995

ATGGATTCGAACAATGCAACGACGGAAGATGAAACAAAACACCTTCCATCAGGGGAAACAACAGTAGGAGAAAGAGTCATGATTAGCACTTTACATGAAAGCATTCTTGGTCACATCCTACCTTTCATTCCAACCGTAGACGCGGTTAGCACTAGCGTCATATCAAGAAGATGGATTGAAGTTTGGACATCCATAACCAGACTAAAGTTTGATGATAGTTTGCTTGGTTCTAAGAAGAAAAAGATGCAAAAGGAACAGTTTGTGAATTTTGTTGAAAAAGTGCTTATTCACTTCACTAACTCAAGCATCCAAAGTTTCTCTCTTTGCTTAACTAGTGATCAATATCATGCATCTCGGATAAGTGAATGGATTTGCTTTATCTTAGAAAGGAGAGTTCAAAAGCTTCATATTCAATATGCTGATAATGTTTTCCTTCCTTCCGATTCACTTTTAAGCTGCAATTCTTTAGTCGAATTGACGCTTCAAATGAGATGTAATCTTAGTTTTCCCATATCTGTTTGTCTCCAAAACCTTCAAAAACTTAGCATTTCTGGGATTAAGTTATTGAGTGATTCCTCCAATTATTCAAAAGATATAACTCTTAGTTTCCCAATTCTCAAAGTGTTTGAAGCTAGAGGATGTGAATGGCCAACGACACACAACATTAGATTACAAGTACCTTTACTGGAGAAGTTTTCCATAGCAATATGGAACCGTCATTCAAATGAATCGTGTAAATTTTCCATGAAAGTTTATTGTTCACGCCTAACGGATTTCTCTTATGAGGGTGATCTTGAACAGGATATTTTTCTGTGTGATTCATCGTCTGTTCGTAATGCTCCTATTGTGATTGTTGTTGATGAAGATAAAAAGGACAGAACGGAAGAACTTTGTTTTCGAGCATATAATCTTTTAAGACAAATCGATGAAGTGGAACGACTGAAATTATTGTTTTACAAGGCATGCTTTTCTTTTGTGATAGTTCAATGGCTATAG

Protein sequence

>MS.gene95995.t1

MDSNNATTEDETKHLPSGETTVGERVMISTLHESILGHILPFIPTVDAVSTSVISRRWIEVWTSITRLKFDDSLLGSKKKKMQKEQFVNFVEKVLIHFTNSSIQSFSLCLTSDQYHASRISEWICFILERRVQKLHIQYADNVFLPSDSLLSCNSLVELTLQMRCNLSFPISVCLQNLQKLSISGIKLLSDSSNYSKDITLSFPILKVFEARGCEWPTTHNIRLQVPLLEKFSIAIWNRHSNESCKFSMKVYCSRLTDFSYEGDLEQDIFLCDSSSVRNAPIVIVVDEDKKDRTEELCFRAYNLLRQIDEVERLKLLFYKACFSFVIVQWL