AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0758130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0758130 MtrunA17_Chr5g0404751 97.674 129 3 0 1 129 1 129 1.67e-92 263
Msa0758130 MtrunA17_Chr2g0299021 57.547 106 45 0 21 126 71 176 1.93e-40 133
Msa0758130 MtrunA17_Chr7g0228311 45.556 90 49 0 40 129 10 99 2.02e-24 90.1
Msa0758130 MtrunA17_Chr3g0117411 43.182 88 50 0 42 129 27 114 9.71e-21 80.9
Msa0758130 MtrunA17_Chr1g0182971 41.304 92 53 1 36 126 86 177 4.85e-20 80.9
Msa0758130 MtrunA17_Chr3g0117391 42.353 85 49 0 42 126 30 114 1.75e-19 78.2
Msa0758130 MtrunA17_Chr7g0268761 38.372 86 52 1 45 129 13 98 1.38e-12 59.7
Msa0758130 MtrunA17_Chr7g0224091 38.636 88 53 1 43 129 32 119 4.91e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0758130 AT1G77370.1 67.500 120 39 0 10 129 11 130 3.55e-54 166
Msa0758130 AT1G77370.2 59.091 132 28 1 24 129 25 156 3.35e-48 152
Msa0758130 AT5G20500.1 52.500 120 57 0 7 126 6 125 1.89e-41 134
Msa0758130 AT5G40370.1 45.977 87 47 0 40 126 10 96 1.03e-22 86.3
Msa0758130 AT5G40370.2 48.750 80 41 0 47 126 42 121 1.88e-20 81.3
Msa0758130 AT4G28730.1 44.565 92 50 1 36 126 73 164 2.26e-20 82.0
Msa0758130 AT2G20270.1 41.304 92 53 1 36 126 78 169 6.00e-19 78.2
Msa0758130 AT5G63030.1 42.683 82 47 0 45 126 31 112 6.67e-18 74.3
Msa0758130 AT4G28730.2 45.588 68 36 1 36 102 73 140 4.52e-13 62.4

Find 43 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCCATTCTCAAACAAAA+AGG 0.217348 5_2:+13230454 None:intergenic
ATTTCGTTGTCATTGTTAAT+TGG 0.231844 5_2:-13228425 Msa0758130:three_prime_UTR
AAATCAGTGTCGTGTCAAAT+TGG 0.237203 5_2:-13230583 Msa0758130:five_prime_UTR
TCAAATTCAGGGTGTTCTTC+TGG 0.247136 5_2:-13229605 Msa0758130:CDS
AGATGATGGGTATCAAATTC+AGG 0.269297 5_2:-13229617 Msa0758130:CDS
GTCCCACAAGTGTTTGTCTA+TGG 0.290060 5_2:-13229562 Msa0758130:CDS
ATTTGACATTGGTTCTTTGT+AGG 0.328186 5_2:+13228229 None:intergenic
GACACGCGTGGAAAACAATA+AGG 0.342799 5_2:-13230623 None:intergenic
TACGTACGGGCAATAGGATT+TGG 0.352701 5_2:+13230364 None:intergenic
TATATATACGTACGGGCAAT+AGG 0.386425 5_2:+13230358 None:intergenic
GGTGTTCTTCTGGATTTGAT+TGG 0.395517 5_2:-13229595 Msa0758130:CDS
GGAAACTGAATTTGAAGCTT+CGG 0.429054 5_2:+13230433 None:intergenic
AATCAGTGTCGTGTCAAATT+GGG 0.440854 5_2:-13230582 Msa0758130:five_prime_UTR
GGAGAAGATGGTTATTCTGT+TGG 0.444652 5_2:+13230385 None:intergenic
TTTGTGCTTGTTTAGATGAT+GGG 0.450399 5_2:-13229630 Msa0758130:intron
AGCAGTGGAGCTCTAATTGT+TGG 0.456983 5_2:-13228453 Msa0758130:three_prime_UTR
AACAGAACTATTCTATTCAA+TGG 0.462801 5_2:+13228184 None:intergenic
GCAATAGGATTTGGAGAAGA+TGG 0.475665 5_2:+13230373 None:intergenic
TCCCATAGACAAACACTTGT+GGG 0.486540 5_2:+13229560 None:intergenic
GATGATGGGTATCAAATTCA+GGG 0.487362 5_2:-13229616 Msa0758130:CDS
ATCTATAATTTGTATGAATG+TGG 0.490361 5_2:-13228650 Msa0758130:three_prime_UTR
TCTATAATTTGTATGAATGT+GGG 0.491540 5_2:-13228649 Msa0758130:three_prime_UTR
ATTGAATCTATCTCAAGTAA+TGG 0.497398 5_2:-13228281 Msa0758130:three_prime_UTR
TTTGTCTATGGGAAGCATAT+TGG 0.501883 5_2:-13229550 Msa0758130:CDS
CGTGGAAAACAATAAGGGTC+TGG 0.507148 5_2:-13230617 None:intergenic
AAATCAAGCTCAACAACAAA+AGG 0.509148 5_2:+13230212 None:intergenic
GTCTATGGGAAGCATATTGG+TGG 0.513655 5_2:-13229547 Msa0758130:CDS
TCCCACAAGTGTTTGTCTAT+GGG 0.535106 5_2:-13229561 Msa0758130:CDS
AATCAAAGAAGTAAAACTAC+AGG 0.537670 5_2:-13228252 Msa0758130:three_prime_UTR
ACACGCGTGGAAAACAATAA+GGG 0.548482 5_2:-13230622 None:intergenic
AAGCATATTGGTGGATCAGA+TGG 0.559694 5_2:-13229538 Msa0758130:intron
GTTTCTGCAATTCACCACTC+TGG 0.562376 5_2:+13228719 None:intergenic
CGAAGGTTTCAAGCTTCTAG+TGG 0.563064 5_2:-13228604 Msa0758130:three_prime_UTR
AAGACAAGACAATTTGACAT+TGG 0.563313 5_2:+13228218 None:intergenic
CTTCGATATACATGAAAATG+CGG 0.575457 5_2:+13228622 None:intergenic
CTATAATTTGTATGAATGTG+GGG 0.594049 5_2:-13228648 Msa0758130:three_prime_UTR
TTCGATATACATGAAAATGC+GGG 0.595061 5_2:+13228623 None:intergenic
TTCCCATAGACAAACACTTG+TGG 0.621034 5_2:+13229559 None:intergenic
TGATTGTATACTTGAGACTG+AGG 0.636878 5_2:-13228682 Msa0758130:three_prime_UTR
GAAAGTACGATCATGAGCAG+TGG 0.651793 5_2:-13228468 Msa0758130:three_prime_UTR
GAAGAATGAAGATGTTACAG+TGG 0.663384 5_2:-13230514 Msa0758130:exon
GTTGTTGAGCTTGATTTGCG+AGG 0.668245 5_2:-13230206 Msa0758130:intron
CTCAGTGCTGCAGTCCAGAG+TGG 0.673667 5_2:-13228733 Msa0758130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTATTGTATTTATTTTTTTA+TGG + chr5_2:13230591-13230610 None:intergenic 10.0%
!!! TATGTGTTTTGATATTTTTA+AGG - chr5_2:13228729-13228748 Msa0758130:CDS 15.0%
!!! AAGGGAATATTTATTATTTT+CGG + chr5_2:13229731-13229750 None:intergenic 15.0%
!!! TTTTTTTCCATATACTTTAA+AGG - chr5_2:13229821-13229840 Msa0758130:intron 15.0%
!! TATCTATATGTCATTATAAA+AGG - chr5_2:13229903-13229922 Msa0758130:intron 15.0%
!!! AATACTTTTTCATGTTTTTT+AGG - chr5_2:13230145-13230164 Msa0758130:intron 15.0%
!!! ATTAAGTTGTTTATCAAACA+AGG + chr5_2:13228586-13228605 None:intergenic 20.0%
!! AATAACCTATATACATAAGA+GGG + chr5_2:13228818-13228837 None:intergenic 20.0%
!! AAATAACCTATATACATAAG+AGG + chr5_2:13228819-13228838 None:intergenic 20.0%
!!! AAACAAAAAAACTGATTTCA+TGG - chr5_2:13228908-13228927 Msa0758130:intron 20.0%
!! TCATATAGTTTAAGAACTAA+AGG + chr5_2:13229584-13229603 None:intergenic 20.0%
!! TAAACTATATGAAAATAACG+AGG - chr5_2:13229592-13229611 Msa0758130:CDS 20.0%
!!! CTTATATTTTTGGATATAGA+GGG + chr5_2:13229646-13229665 None:intergenic 20.0%
!! CTCTATATCCAAAAATATAA+GGG - chr5_2:13229645-13229664 Msa0758130:intron 20.0%
!!! TGATAGAAATTTTTTATGAG+AGG - chr5_2:13229697-13229716 Msa0758130:intron 20.0%
!!! TATTTAGCCTTTAAAGTATA+TGG + chr5_2:13229831-13229850 None:intergenic 20.0%
!! TATGTCATTATAAAAGGTTA+AGG - chr5_2:13229909-13229928 Msa0758130:intron 20.0%
!!! ATCTATAATTTGTATGAATG+TGG - chr5_2:13230073-13230092 Msa0758130:intron 20.0%
!!! TCTATAATTTGTATGAATGT+GGG - chr5_2:13230074-13230093 Msa0758130:intron 20.0%
!!! ATGTTTTTTAGGTATTTACT+AGG - chr5_2:13230156-13230175 Msa0758130:intron 20.0%
!!! ATGTTTTCATTTTGTTCTAT+AGG - chr5_2:13230233-13230252 Msa0758130:CDS 20.0%
!!! AATTGTAGTTTTCAATAATC+TGG - chr5_2:13230326-13230345 Msa0758130:intron 20.0%
! AAGAGAGTTATATATACGTA+CGG + chr5_2:13228376-13228395 None:intergenic 25.0%
! TTCAATTATTATCACGTGTT+AGG - chr5_2:13228401-13228420 Msa0758130:three_prime_UTR 25.0%
! TCAATTATTATCACGTGTTA+GGG - chr5_2:13228402-13228421 Msa0758130:three_prime_UTR 25.0%
! GTTTAATTAATATGCTCTGT+CGG - chr5_2:13228639-13228658 Msa0758130:three_prime_UTR 25.0%
! GACAAGAATTCAAATAGTAT+CGG + chr5_2:13228789-13228808 None:intergenic 25.0%
!! TTTTCATAAGTGTTTATGTC+AGG - chr5_2:13228993-13229012 Msa0758130:intron 25.0%
! AATGATAACAGTTGTCTATT+TGG + chr5_2:13229041-13229060 None:intergenic 25.0%
!! TTAAGTTGTTTATCCAAACA+AGG + chr5_2:13229310-13229329 None:intergenic 25.0%
! TATGAAAATAACGAGGAATT+TGG - chr5_2:13229599-13229618 Msa0758130:CDS 25.0%
!!! CCTTATATTTTTGGATATAG+AGG + chr5_2:13229647-13229666 None:intergenic 25.0%
! CCTCTATATCCAAAAATATA+AGG - chr5_2:13229644-13229663 Msa0758130:intron 25.0%
! TATATCCAAAAATATAAGGG+TGG - chr5_2:13229648-13229667 Msa0758130:intron 25.0%
!! ATACTTTAAAGGCTAAATAG+AGG - chr5_2:13229832-13229851 Msa0758130:intron 25.0%
! TAATGAAGGGATTAAATTGA+AGG - chr5_2:13229864-13229883 Msa0758130:intron 25.0%
!! CTATAATTTGTATGAATGTG+GGG - chr5_2:13230075-13230094 Msa0758130:intron 25.0%
! ATTTCGTTGTCATTGTTAAT+TGG - chr5_2:13230298-13230317 Msa0758130:intron 25.0%
! ATTGAATCTATCTCAAGTAA+TGG - chr5_2:13230442-13230461 Msa0758130:CDS 25.0%
! AATCAAAGAAGTAAAACTAC+AGG - chr5_2:13230471-13230490 Msa0758130:CDS 25.0%
! AACAGAACTATTCTATTCAA+TGG + chr5_2:13230542-13230561 None:intergenic 25.0%
!!! TGTTTTTGTACCAAACAAAA+TGG - chr5_2:13230558-13230577 Msa0758130:five_prime_UTR 25.0%
CTCAAACAAAAAGGGTAAAA+CGG + chr5_2:13228263-13228282 None:intergenic 30.0%
!! TTACCCTTTTTGTTTGAGAA+TGG - chr5_2:13228265-13228284 Msa0758130:three_prime_UTR 30.0%
GTTCCATTCTCAAACAAAAA+GGG + chr5_2:13228271-13228290 None:intergenic 30.0%
AGAGAGTTATATATACGTAC+GGG + chr5_2:13228375-13228394 None:intergenic 30.0%
! AATGTTTCTCTGATTTTCGT+AGG - chr5_2:13228443-13228462 Msa0758130:three_prime_UTR 30.0%
AAATCAAGCTCAACAACAAA+AGG + chr5_2:13228514-13228533 None:intergenic 30.0%
!! TATATACATAAGAGGGTATC+AGG + chr5_2:13228811-13228830 None:intergenic 30.0%
TGATACCCTCTTATGTATAT+AGG - chr5_2:13228810-13228829 Msa0758130:intron 30.0%
TAACAGTTGTCTATTTGGAT+TGG + chr5_2:13229036-13229055 None:intergenic 30.0%
! TTTTGTGCTTGTTTAGATGA+TGG - chr5_2:13229092-13229111 Msa0758130:intron 30.0%
TTTGTGCTTGTTTAGATGAT+GGG - chr5_2:13229093-13229112 Msa0758130:intron 30.0%
!! TTCTTAGACTTTTGCTTTTC+TGG - chr5_2:13229219-13229238 Msa0758130:intron 30.0%
!! TCTTAGACTTTTGCTTTTCT+GGG - chr5_2:13229220-13229239 Msa0758130:intron 30.0%
TTTCATACTTAGTCCTTGTT+TGG - chr5_2:13229294-13229313 Msa0758130:intron 30.0%
! ACTTTCCACCCTTATATTTT+TGG + chr5_2:13229656-13229675 None:intergenic 30.0%
! ACAAATTTTCACACACTCAA+GGG + chr5_2:13229749-13229768 None:intergenic 30.0%
CATTAGTACTTAAGATGCTA+GGG + chr5_2:13229781-13229800 None:intergenic 30.0%
TCATTAGTACTTAAGATGCT+AGG + chr5_2:13229782-13229801 None:intergenic 30.0%
TCTTAAGTACTAATGAGACA+AGG - chr5_2:13229786-13229805 Msa0758130:intron 30.0%
CTTAAGTACTAATGAGACAA+GGG - chr5_2:13229787-13229806 Msa0758130:intron 30.0%
TTAAGTACTAATGAGACAAG+GGG - chr5_2:13229788-13229807 Msa0758130:intron 30.0%
! AGAGGAGATAATGATAATGA+AGG - chr5_2:13229850-13229869 Msa0758130:intron 30.0%
! GAGGAGATAATGATAATGAA+GGG - chr5_2:13229851-13229870 Msa0758130:intron 30.0%
ATGAGCAAACTTTGTAAGAA+TGG + chr5_2:13229956-13229975 None:intergenic 30.0%
TTCGATATACATGAAAATGC+GGG + chr5_2:13230103-13230122 None:intergenic 30.0%
CTTCGATATACATGAAAATG+CGG + chr5_2:13230104-13230123 None:intergenic 30.0%
!! ATTTGACATTGGTTCTTTGT+AGG + chr5_2:13230497-13230516 None:intergenic 30.0%
AAGACAAGACAATTTGACAT+TGG + chr5_2:13230508-13230527 None:intergenic 30.0%
AAATCAGTGTCGTGTCAAAT+TGG - chr5_2:13228140-13228159 Msa0758130:three_prime_UTR 35.0%
AATCAGTGTCGTGTCAAATT+GGG - chr5_2:13228141-13228160 Msa0758130:three_prime_UTR 35.0%
GAAGAATGAAGATGTTACAG+TGG - chr5_2:13228209-13228228 Msa0758130:three_prime_UTR 35.0%
GGTTCCATTCTCAAACAAAA+AGG + chr5_2:13228272-13228291 None:intergenic 35.0%
! GGAAACTGAATTTGAAGCTT+CGG + chr5_2:13228293-13228312 None:intergenic 35.0%
!! GATTGCGTTTTGAACAAAAG+CGG + chr5_2:13228314-13228333 None:intergenic 35.0%
TATATATACGTACGGGCAAT+AGG + chr5_2:13228368-13228387 None:intergenic 35.0%
TAACGAGACAACACAATCAT+CGG + chr5_2:13228688-13228707 None:intergenic 35.0%
! ATTGTTTTCGCGTAAGCTAT+AGG - chr5_2:13228855-13228874 Msa0758130:intron 35.0%
!! AGATGATGGGTATCAAATTC+AGG - chr5_2:13229106-13229125 Msa0758130:intron 35.0%
!! GATGATGGGTATCAAATTCA+GGG - chr5_2:13229107-13229126 Msa0758130:intron 35.0%
! TTTGTCTATGGGAAGCATAT+TGG - chr5_2:13229173-13229192 Msa0758130:intron 35.0%
! GACAAATTTTCACACACTCA+AGG + chr5_2:13229750-13229769 None:intergenic 35.0%
TTAAGATGCTAGGGATTAAC+AGG + chr5_2:13229772-13229791 None:intergenic 35.0%
TGGATAGAGAAACACAAAGA+GGG + chr5_2:13229936-13229955 None:intergenic 35.0%
ATGGATAGAGAAACACAAAG+AGG + chr5_2:13229937-13229956 None:intergenic 35.0%
TGATTGTATACTTGAGACTG+AGG - chr5_2:13230041-13230060 Msa0758130:intron 35.0%
! CGCATTTTCATGTATATCGA+AGG - chr5_2:13230102-13230121 Msa0758130:intron 35.0%
!!! ACATGTACTTTTAGCTTGTC+TGG - chr5_2:13230185-13230204 Msa0758130:intron 35.0%
!! TGGTACAAAGCCATTTTGTT+TGG + chr5_2:13230571-13230590 None:intergenic 35.0%
GGAGAAGATGGTTATTCTGT+TGG + chr5_2:13228341-13228360 None:intergenic 40.0%
GCAATAGGATTTGGAGAAGA+TGG + chr5_2:13228353-13228372 None:intergenic 40.0%
! ACTGATTTCATGGTCTGTCA+TGG - chr5_2:13228918-13228937 Msa0758130:intron 40.0%
! TCAAATTCAGGGTGTTCTTC+TGG - chr5_2:13229118-13229137 Msa0758130:intron 40.0%
GGTGTTCTTCTGGATTTGAT+TGG - chr5_2:13229128-13229147 Msa0758130:intron 40.0%
TCCCACAAGTGTTTGTCTAT+GGG - chr5_2:13229162-13229181 Msa0758130:intron 40.0%
TCCCATAGACAAACACTTGT+GGG + chr5_2:13229166-13229185 None:intergenic 40.0%
TTCCCATAGACAAACACTTG+TGG + chr5_2:13229167-13229186 None:intergenic 40.0%
! AAGCATATTGGTGGATCAGA+TGG - chr5_2:13229185-13229204 Msa0758130:intron 40.0%
GTGGAAAGTGAGAGAAGATA+GGG - chr5_2:13229667-13229686 Msa0758130:intron 40.0%
TACGTACGGGCAATAGGATT+TGG + chr5_2:13228362-13228381 None:intergenic 45.0%
!! GTTGTTGAGCTTGATTTGCG+AGG - chr5_2:13228517-13228536 Msa0758130:three_prime_UTR 45.0%
GTCCCACAAGTGTTTGTCTA+TGG - chr5_2:13229161-13229180 Msa0758130:intron 45.0%
! GTCTATGGGAAGCATATTGG+TGG - chr5_2:13229176-13229195 Msa0758130:intron 45.0%
AGGGATGCATGTTTGAGAGA+GGG + chr5_2:13229627-13229646 None:intergenic 45.0%
GGTGGAAAGTGAGAGAAGAT+AGG - chr5_2:13229666-13229685 Msa0758130:intron 45.0%
GTTTCTGCAATTCACCACTC+TGG + chr5_2:13230007-13230026 None:intergenic 45.0%
CGAAGGTTTCAAGCTTCTAG+TGG - chr5_2:13230119-13230138 Msa0758130:intron 45.0%
! GAAAGTACGATCATGAGCAG+TGG - chr5_2:13230255-13230274 Msa0758130:CDS 45.0%
! AGCAGTGGAGCTCTAATTGT+TGG - chr5_2:13230270-13230289 Msa0758130:intron 45.0%
GAGGGATGCATGTTTGAGAG+AGG + chr5_2:13229628-13229647 None:intergenic 50.0%
CTCAGTGCTGCAGTCCAGAG+TGG - chr5_2:13229990-13230009 Msa0758130:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 13228112 13230633 13228112 ID=Msa0758130;Name=Msa0758130
chr5_2 mRNA 13228112 13230633 13228112 ID=Msa0758130-mRNA-1;Parent=Msa0758130;Name=Msa0758130-mRNA-1;_AED=0.17;_eAED=0.17;_QI=102|1|1|1|1|1|4|590|129
chr5_2 exon 13228112 13228757 13228112 ID=Msa0758130-mRNA-1:exon:5970;Parent=Msa0758130-mRNA-1
chr5_2 exon 13229539 13229637 13229539 ID=Msa0758130-mRNA-1:exon:5969;Parent=Msa0758130-mRNA-1
chr5_2 exon 13230207 13230280 13230207 ID=Msa0758130-mRNA-1:exon:5968;Parent=Msa0758130-mRNA-1
chr5_2 exon 13230371 13230633 13230371 ID=Msa0758130-mRNA-1:exon:5967;Parent=Msa0758130-mRNA-1
chr5_2 five_prime_UTR 13230532 13230633 13230532 ID=Msa0758130-mRNA-1:five_prime_utr;Parent=Msa0758130-mRNA-1
chr5_2 CDS 13230371 13230531 13230371 ID=Msa0758130-mRNA-1:cds;Parent=Msa0758130-mRNA-1
chr5_2 CDS 13230207 13230280 13230207 ID=Msa0758130-mRNA-1:cds;Parent=Msa0758130-mRNA-1
chr5_2 CDS 13229539 13229637 13229539 ID=Msa0758130-mRNA-1:cds;Parent=Msa0758130-mRNA-1
chr5_2 CDS 13228702 13228757 13228702 ID=Msa0758130-mRNA-1:cds;Parent=Msa0758130-mRNA-1
chr5_2 three_prime_UTR 13228112 13228701 13228112 ID=Msa0758130-mRNA-1:three_prime_utr;Parent=Msa0758130-mRNA-1
Gene Sequence

>Msa0758130

ATGAAGATGTTACAGTGGAGCATGTTCATAACTCTCACGTTATTGACCGTTTTACCCTTTTTGTTTGAGAATGGAACCGAAGCTTCAAATTCAGTTTCCGCTTTTGTTCAAAACGCAATCTACTCCAACAGAATAACCATCTTCTCCAAATCCTATTGCCCGTATTGTTTACGTGCTAAACGTGTTTTTGTTGAACTAAATGAACAACCTTTTGTTGTTGAGCTTGATTTGCGAGATGATGGGTATCAAATTCAGGGTGTTCTTCTGGATTTGATTGGTAGAAGAACAGTCCCACAAGTGTTTGTCTATGGGAAGCATATTGGTGGATCAGATGATCTCAGTGCTGCAGTCCAGAGTGGTGAATTGCAGAAACTTCTCAAATCAAGTTGA

Protein sequence

>Msa0758130

MKMLQWSMFITLTLLTVLPFLFENGTEASNSVSAFVQNAIYSNRITIFSKSYCPYCLRAKRVFVELNEQPFVVELDLRDDGYQIQGVLLDLIGRRTVPQVFVYGKHIGGSDDLSAAVQSGELQKLLKSS*