AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0566020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566020 MtrunA17_Chr8g0380491 96.226 106 4 0 1 106 1 106 1.91e-70 206
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566020 AT1G55365.1 49.275 69 33 1 1 67 1 69 3.65e-16 68.9

Find 22 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGATGATGCTTCTGCTTC+TGG 0.168743 4_1:+66938033 Msa0566020:CDS
AAAAGCTGATCACTGTTTAT+TGG 0.179925 4_1:-66937877 None:intergenic
TAAGAAGAGTAGAAGGAATA+AGG 0.282580 4_1:-66937979 None:intergenic
AATAAACAGTGATCAGCTTT+TGG 0.289636 4_1:+66937879 Msa0566020:CDS
AAGAAGATAAGAAGAGTAGA+AGG 0.345054 4_1:-66937986 None:intergenic
CCACAAGACAGACAAGAAAA+TGG 0.358339 4_1:+66937777 Msa0566020:exon
CAAAATTATGAGTATAATCA+TGG 0.401432 4_1:+66939841 Msa0566020:three_prime_UTR
TATGAGTATAATCATGGGAT+TGG 0.452870 4_1:+66939847 Msa0566020:three_prime_UTR
TGTTTCCCTCTTCATCAACT+TGG 0.458525 4_1:+66937913 Msa0566020:CDS
GTGTAGAAATCAAATTATCT+AGG 0.474489 4_1:+66937675 Msa0566020:five_prime_UTR
GAGTAGAAGGAATAAGGTTG+AGG 0.494913 4_1:-66937973 None:intergenic
CACTCATTCTCAAGGAGTCA+AGG 0.538743 4_1:-66937655 None:intergenic
TTATCTAGGTGAGGTGAAGT+AGG 0.543154 4_1:+66937689 Msa0566020:five_prime_UTR
GATTTCTACACTCATTCTCA+AGG 0.553797 4_1:-66937663 None:intergenic
AGACGACCAAGTTGATGAAG+AGG 0.585076 4_1:-66937919 None:intergenic
AAAATTATGAGTATAATCAT+GGG 0.595414 4_1:+66939842 Msa0566020:three_prime_UTR
GACGACCAAGTTGATGAAGA+GGG 0.605275 4_1:-66937918 None:intergenic
GAGGTGAAGTAGGTAGCAGA+AGG 0.607950 4_1:+66937699 Msa0566020:five_prime_UTR
TGTCTGCACAAACGTGGCAA+TGG 0.611212 4_1:+66937836 Msa0566020:CDS
GAAATCAAATTATCTAGGTG+AGG 0.614759 4_1:+66937680 Msa0566020:five_prime_UTR
GAAAATGGTGTTGAACACTC+AGG 0.614987 4_1:+66937792 Msa0566020:exon
CACGTGTGTCTGCACAAACG+TGG 0.716541 4_1:+66937830 Msa0566020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAATTAATATAATATCAA+AGG - chr4_1:66938649-66938668 None:intergenic 10.0%
!! TTTGATATTATATTAATTGA+AGG + chr4_1:66938648-66938667 Msa0566020:intron 10.0%
!! TTCAATTAATATAATATCAA+AGG - chr4_1:66938649-66938668 None:intergenic 10.0%
!! TTTGATATTATATTAATTGA+AGG + chr4_1:66938648-66938667 Msa0566020:intron 10.0%
!!! TTTTCTTCATCTTTTTTTAA+TGG + chr4_1:66938121-66938140 Msa0566020:intron 15.0%
!!! TTTCTTCATCTTTTTTTAAT+GGG + chr4_1:66938122-66938141 Msa0566020:intron 15.0%
!!! TTATTTTTACATTAATTGAC+TGG + chr4_1:66938169-66938188 Msa0566020:intron 15.0%
!!! TATTTTTACATTAATTGACT+GGG + chr4_1:66938170-66938189 Msa0566020:intron 15.0%
!! TTAATGAATAATGTATGTAT+AGG + chr4_1:66938526-66938545 Msa0566020:intron 15.0%
!!! ATTATTTTAACGTACTATAT+TGG + chr4_1:66938953-66938972 Msa0566020:intron 15.0%
!!! GCAGATTAAAATTTTAAAAT+AGG - chr4_1:66939237-66939256 None:intergenic 15.0%
!!! TTTTTCTTTAATATTTCCAT+TGG + chr4_1:66939465-66939484 Msa0566020:intron 15.0%
!! AAAATTATGAGTATAATCAT+GGG + chr4_1:66939842-66939861 Msa0566020:three_prime_UTR 15.0%
!!! TTTTCTTCATCTTTTTTTAA+TGG + chr4_1:66938121-66938140 Msa0566020:intron 15.0%
!!! TTTCTTCATCTTTTTTTAAT+GGG + chr4_1:66938122-66938141 Msa0566020:intron 15.0%
!!! TTATTTTTACATTAATTGAC+TGG + chr4_1:66938169-66938188 Msa0566020:intron 15.0%
!!! TATTTTTACATTAATTGACT+GGG + chr4_1:66938170-66938189 Msa0566020:intron 15.0%
!! TTAATGAATAATGTATGTAT+AGG + chr4_1:66938526-66938545 Msa0566020:intron 15.0%
!!! ATTATTTTAACGTACTATAT+TGG + chr4_1:66938953-66938972 Msa0566020:intron 15.0%
!!! GCAGATTAAAATTTTAAAAT+AGG - chr4_1:66939237-66939256 None:intergenic 15.0%
!!! TTTTTCTTTAATATTTCCAT+TGG + chr4_1:66939465-66939484 Msa0566020:intron 15.0%
!! AAAATTATGAGTATAATCAT+GGG + chr4_1:66939842-66939861 Msa0566020:three_prime_UTR 15.0%
!! ATAATATTGTTTGATGAACT+TGG + chr4_1:66938287-66938306 Msa0566020:intron 20.0%
!! ATATAAAAATGATAGCCTAT+AGG + chr4_1:66938699-66938718 Msa0566020:intron 20.0%
!!! AGAATTGCTTTCTTATATTT+TGG + chr4_1:66939138-66939157 Msa0566020:intron 20.0%
!!! GAATTGCTTTCTTATATTTT+GGG + chr4_1:66939139-66939158 Msa0566020:intron 20.0%
!!! TAAAATTTTAAAATAGGAGC+CGG - chr4_1:66939231-66939250 None:intergenic 20.0%
!! GTTCACAAAAAAAAAAAAGA+AGG - chr4_1:66939430-66939449 None:intergenic 20.0%
!!! TCTTTTTTTTTTTTGTGAAC+AGG + chr4_1:66939430-66939449 Msa0566020:intron 20.0%
!! TATTAAAGAAAAAGTTGGAA+TGG - chr4_1:66939458-66939477 None:intergenic 20.0%
!! GGAAATATTAAAGAAAAAGT+TGG - chr4_1:66939463-66939482 None:intergenic 20.0%
!! CAAAATTATGAGTATAATCA+TGG + chr4_1:66939841-66939860 Msa0566020:three_prime_UTR 20.0%
!! ATAATATTGTTTGATGAACT+TGG + chr4_1:66938287-66938306 Msa0566020:intron 20.0%
!! ATATAAAAATGATAGCCTAT+AGG + chr4_1:66938699-66938718 Msa0566020:intron 20.0%
!!! AGAATTGCTTTCTTATATTT+TGG + chr4_1:66939138-66939157 Msa0566020:intron 20.0%
!!! GAATTGCTTTCTTATATTTT+GGG + chr4_1:66939139-66939158 Msa0566020:intron 20.0%
!!! TAAAATTTTAAAATAGGAGC+CGG - chr4_1:66939231-66939250 None:intergenic 20.0%
!! GTTCACAAAAAAAAAAAAGA+AGG - chr4_1:66939430-66939449 None:intergenic 20.0%
!!! TCTTTTTTTTTTTTGTGAAC+AGG + chr4_1:66939430-66939449 Msa0566020:intron 20.0%
!! TATTAAAGAAAAAGTTGGAA+TGG - chr4_1:66939458-66939477 None:intergenic 20.0%
!! GGAAATATTAAAGAAAAAGT+TGG - chr4_1:66939463-66939482 None:intergenic 20.0%
!! CAAAATTATGAGTATAATCA+TGG + chr4_1:66939841-66939860 Msa0566020:three_prime_UTR 20.0%
! GTGTAGAAATCAAATTATCT+AGG + chr4_1:66937675-66937694 Msa0566020:five_prime_UTR 25.0%
!! TGTTGTTGCTTAATGTTTAT+TGG + chr4_1:66938225-66938244 Msa0566020:intron 25.0%
!! GTTGTTGCTTAATGTTTATT+GGG + chr4_1:66938226-66938245 Msa0566020:intron 25.0%
!!! TTTGATCCATTTTGGTTTTT+TGG + chr4_1:66938457-66938476 Msa0566020:intron 25.0%
! TTATTGTGTGAGTTCTATTT+AGG + chr4_1:66938560-66938579 Msa0566020:intron 25.0%
! TTCTATGAATCTTTACCTAT+AGG - chr4_1:66938717-66938736 None:intergenic 25.0%
! TTATTCAATGAAGCAAAAAG+AGG + chr4_1:66938778-66938797 Msa0566020:intron 25.0%
! AGTGAGAATATTATGTAAGT+AGG - chr4_1:66938810-66938829 None:intergenic 25.0%
!! ACAAAACCCTAATTAAGTTT+TGG + chr4_1:66938847-66938866 Msa0566020:intron 25.0%
! GTACAACCAAAACTTAATTA+GGG - chr4_1:66938856-66938875 None:intergenic 25.0%
! TGTACAACCAAAACTTAATT+AGG - chr4_1:66938857-66938876 None:intergenic 25.0%
!! AACCAAAATCTTTTCACATT+AGG - chr4_1:66938911-66938930 None:intergenic 25.0%
! ATCTACTTGGATATAAGAAA+TGG - chr4_1:66938995-66939014 None:intergenic 25.0%
! ACTCAAAAGAAAACGATAAA+TGG - chr4_1:66939048-66939067 None:intergenic 25.0%
! TAAATAAGAAGCAACTTAGA+TGG - chr4_1:66939089-66939108 None:intergenic 25.0%
!! AAAAGAGTGTAAAGTTTACT+GGG - chr4_1:66939196-66939215 None:intergenic 25.0%
!! TAAACTTTACACTCTTTTGT+AGG + chr4_1:66939198-66939217 Msa0566020:intron 25.0%
!!! AAAATTTTAAAATAGGAGCC+GGG - chr4_1:66939230-66939249 None:intergenic 25.0%
! AAGAACTTATGCATCAATTT+GGG - chr4_1:66939263-66939282 None:intergenic 25.0%
! AAAGAACTTATGCATCAATT+TGG - chr4_1:66939264-66939283 None:intergenic 25.0%
! AAGAGAGTTTAAACATATGA+GGG - chr4_1:66939394-66939413 None:intergenic 25.0%
! TAAGAGAGTTTAAACATATG+AGG - chr4_1:66939395-66939414 None:intergenic 25.0%
! TCATATGTTTAAACTCTCTT+AGG + chr4_1:66939394-66939413 Msa0566020:intron 25.0%
!! ATATTTCCATTGGTAATTGT+TGG + chr4_1:66939475-66939494 Msa0566020:intron 25.0%
!! TAAACCTTGTTATCACATAT+AGG + chr4_1:66939621-66939640 Msa0566020:intron 25.0%
!! CAAGTCTTTTCCAATTTATT+AGG - chr4_1:66939654-66939673 None:intergenic 25.0%
! TAGAAATTCAAATCTTACAG+AGG - chr4_1:66939734-66939753 None:intergenic 25.0%
!!! ATTTCTAGTTTCTGAAGAAT+AGG + chr4_1:66939747-66939766 Msa0566020:intron 25.0%
! GTGTAGAAATCAAATTATCT+AGG + chr4_1:66937675-66937694 Msa0566020:five_prime_UTR 25.0%
!! TGTTGTTGCTTAATGTTTAT+TGG + chr4_1:66938225-66938244 Msa0566020:intron 25.0%
!! GTTGTTGCTTAATGTTTATT+GGG + chr4_1:66938226-66938245 Msa0566020:intron 25.0%
!!! TTTGATCCATTTTGGTTTTT+TGG + chr4_1:66938457-66938476 Msa0566020:intron 25.0%
! TTATTGTGTGAGTTCTATTT+AGG + chr4_1:66938560-66938579 Msa0566020:intron 25.0%
! TTCTATGAATCTTTACCTAT+AGG - chr4_1:66938717-66938736 None:intergenic 25.0%
! TTATTCAATGAAGCAAAAAG+AGG + chr4_1:66938778-66938797 Msa0566020:intron 25.0%
! AGTGAGAATATTATGTAAGT+AGG - chr4_1:66938810-66938829 None:intergenic 25.0%
!! ACAAAACCCTAATTAAGTTT+TGG + chr4_1:66938847-66938866 Msa0566020:intron 25.0%
! GTACAACCAAAACTTAATTA+GGG - chr4_1:66938856-66938875 None:intergenic 25.0%
! TGTACAACCAAAACTTAATT+AGG - chr4_1:66938857-66938876 None:intergenic 25.0%
!! AACCAAAATCTTTTCACATT+AGG - chr4_1:66938911-66938930 None:intergenic 25.0%
! ATCTACTTGGATATAAGAAA+TGG - chr4_1:66938995-66939014 None:intergenic 25.0%
! ACTCAAAAGAAAACGATAAA+TGG - chr4_1:66939048-66939067 None:intergenic 25.0%
! TAAATAAGAAGCAACTTAGA+TGG - chr4_1:66939089-66939108 None:intergenic 25.0%
!! AAAAGAGTGTAAAGTTTACT+GGG - chr4_1:66939196-66939215 None:intergenic 25.0%
!! TAAACTTTACACTCTTTTGT+AGG + chr4_1:66939198-66939217 Msa0566020:intron 25.0%
!!! AAAATTTTAAAATAGGAGCC+GGG - chr4_1:66939230-66939249 None:intergenic 25.0%
! AAGAACTTATGCATCAATTT+GGG - chr4_1:66939263-66939282 None:intergenic 25.0%
! AAAGAACTTATGCATCAATT+TGG - chr4_1:66939264-66939283 None:intergenic 25.0%
! AAGAGAGTTTAAACATATGA+GGG - chr4_1:66939394-66939413 None:intergenic 25.0%
! TAAGAGAGTTTAAACATATG+AGG - chr4_1:66939395-66939414 None:intergenic 25.0%
! TCATATGTTTAAACTCTCTT+AGG + chr4_1:66939394-66939413 Msa0566020:intron 25.0%
!! ATATTTCCATTGGTAATTGT+TGG + chr4_1:66939475-66939494 Msa0566020:intron 25.0%
!! TAAACCTTGTTATCACATAT+AGG + chr4_1:66939621-66939640 Msa0566020:intron 25.0%
!! CAAGTCTTTTCCAATTTATT+AGG - chr4_1:66939654-66939673 None:intergenic 25.0%
! TAGAAATTCAAATCTTACAG+AGG - chr4_1:66939734-66939753 None:intergenic 25.0%
!!! ATTTCTAGTTTCTGAAGAAT+AGG + chr4_1:66939747-66939766 Msa0566020:intron 25.0%
GAAATCAAATTATCTAGGTG+AGG + chr4_1:66937680-66937699 Msa0566020:five_prime_UTR 30.0%
AAAAGCTGATCACTGTTTAT+TGG - chr4_1:66937880-66937899 None:intergenic 30.0%
! AATAAACAGTGATCAGCTTT+TGG + chr4_1:66937879-66937898 Msa0566020:CDS 30.0%
TAAGAAGAGTAGAAGGAATA+AGG - chr4_1:66937982-66938001 None:intergenic 30.0%
AAGAAGATAAGAAGAGTAGA+AGG - chr4_1:66937989-66938008 None:intergenic 30.0%
AATTGTACAATCTGATCTGA+TGG + chr4_1:66938330-66938349 Msa0566020:intron 30.0%
TTGTCTGATGAACTTTCTAA+CGG + chr4_1:66938373-66938392 Msa0566020:intron 30.0%
TTCCAACAATTATAGCCTAT+AGG + chr4_1:66938402-66938421 Msa0566020:intron 30.0%
TACCTATAGGCTATAATTGT+TGG - chr4_1:66938407-66938426 None:intergenic 30.0%
GAACTTCCAAAAAACCAAAA+TGG - chr4_1:66938466-66938485 None:intergenic 30.0%
!!! GGTTTTTTGGAAGTTCAAAT+CGG + chr4_1:66938470-66938489 Msa0566020:intron 30.0%
!!! GTTTTTTGGAAGTTCAAATC+GGG + chr4_1:66938471-66938490 Msa0566020:intron 30.0%
AATGTATGTATAGGATGAGA+TGG + chr4_1:66938535-66938554 Msa0566020:intron 30.0%
AATTCATGCACATCTAACAA+TGG - chr4_1:66938596-66938615 None:intergenic 30.0%
! AAATCTTTTCACATTAGGTG+AGG - chr4_1:66938906-66938925 None:intergenic 30.0%
! CACCTAATGTGAAAAGATTT+TGG + chr4_1:66938906-66938925 Msa0566020:intron 30.0%
!!! TGAAAAGATTTTGGTTGTAG+TGG + chr4_1:66938915-66938934 Msa0566020:intron 30.0%
!!! TTTTGGGATTTGCTATCATT+TGG + chr4_1:66939155-66939174 Msa0566020:intron 30.0%
!! TTTGGGATTTGCTATCATTT+GGG + chr4_1:66939156-66939175 Msa0566020:intron 30.0%
! CAAAAGAGTGTAAAGTTTAC+TGG - chr4_1:66939197-66939216 None:intergenic 30.0%
GAATTTCCAACAATTACCAA+TGG - chr4_1:66939484-66939503 None:intergenic 30.0%
!! ACTCTGAAAACTACTGTTTT+GGG - chr4_1:66939568-66939587 None:intergenic 30.0%
! AGAGTGTCTGTAGATAATAA+GGG + chr4_1:66939583-66939602 Msa0566020:intron 30.0%
ATATATGTCGTATCTTTGTC+TGG + chr4_1:66939697-66939716 Msa0566020:intron 30.0%
ATTCAAATCTTACAGAGGAT+AGG - chr4_1:66939729-66939748 None:intergenic 30.0%
TATGAGTATAATCATGGGAT+TGG + chr4_1:66939847-66939866 Msa0566020:three_prime_UTR 30.0%
GAAATCAAATTATCTAGGTG+AGG + chr4_1:66937680-66937699 Msa0566020:five_prime_UTR 30.0%
AAAAGCTGATCACTGTTTAT+TGG - chr4_1:66937880-66937899 None:intergenic 30.0%
! AATAAACAGTGATCAGCTTT+TGG + chr4_1:66937879-66937898 Msa0566020:CDS 30.0%
TAAGAAGAGTAGAAGGAATA+AGG - chr4_1:66937982-66938001 None:intergenic 30.0%
AAGAAGATAAGAAGAGTAGA+AGG - chr4_1:66937989-66938008 None:intergenic 30.0%
AATTGTACAATCTGATCTGA+TGG + chr4_1:66938330-66938349 Msa0566020:intron 30.0%
TTGTCTGATGAACTTTCTAA+CGG + chr4_1:66938373-66938392 Msa0566020:intron 30.0%
TTCCAACAATTATAGCCTAT+AGG + chr4_1:66938402-66938421 Msa0566020:intron 30.0%
TACCTATAGGCTATAATTGT+TGG - chr4_1:66938407-66938426 None:intergenic 30.0%
GAACTTCCAAAAAACCAAAA+TGG - chr4_1:66938466-66938485 None:intergenic 30.0%
!!! GGTTTTTTGGAAGTTCAAAT+CGG + chr4_1:66938470-66938489 Msa0566020:intron 30.0%
!!! GTTTTTTGGAAGTTCAAATC+GGG + chr4_1:66938471-66938490 Msa0566020:intron 30.0%
AATGTATGTATAGGATGAGA+TGG + chr4_1:66938535-66938554 Msa0566020:intron 30.0%
AATTCATGCACATCTAACAA+TGG - chr4_1:66938596-66938615 None:intergenic 30.0%
! AAATCTTTTCACATTAGGTG+AGG - chr4_1:66938906-66938925 None:intergenic 30.0%
! CACCTAATGTGAAAAGATTT+TGG + chr4_1:66938906-66938925 Msa0566020:intron 30.0%
!!! TGAAAAGATTTTGGTTGTAG+TGG + chr4_1:66938915-66938934 Msa0566020:intron 30.0%
!!! TTTTGGGATTTGCTATCATT+TGG + chr4_1:66939155-66939174 Msa0566020:intron 30.0%
!! TTTGGGATTTGCTATCATTT+GGG + chr4_1:66939156-66939175 Msa0566020:intron 30.0%
! CAAAAGAGTGTAAAGTTTAC+TGG - chr4_1:66939197-66939216 None:intergenic 30.0%
GAATTTCCAACAATTACCAA+TGG - chr4_1:66939484-66939503 None:intergenic 30.0%
!! ACTCTGAAAACTACTGTTTT+GGG - chr4_1:66939568-66939587 None:intergenic 30.0%
! AGAGTGTCTGTAGATAATAA+GGG + chr4_1:66939583-66939602 Msa0566020:intron 30.0%
ATATATGTCGTATCTTTGTC+TGG + chr4_1:66939697-66939716 Msa0566020:intron 30.0%
ATTCAAATCTTACAGAGGAT+AGG - chr4_1:66939729-66939748 None:intergenic 30.0%
TATGAGTATAATCATGGGAT+TGG + chr4_1:66939847-66939866 Msa0566020:three_prime_UTR 30.0%
GATTTCTACACTCATTCTCA+AGG - chr4_1:66937666-66937685 None:intergenic 35.0%
ACAATGATTCAGCTACCTAT+AGG - chr4_1:66938420-66938439 None:intergenic 35.0%
AGCTGAATCATTGTATTAGC+AGG + chr4_1:66938426-66938445 Msa0566020:intron 35.0%
!! TGCTAGGTTTTGATCCATTT+TGG + chr4_1:66938449-66938468 Msa0566020:intron 35.0%
! ATTGGTCTACTTGGTTAGAT+GGG + chr4_1:66938494-66938513 Msa0566020:intron 35.0%
!!! CTTAATTAGGGTTTTGTTCG+TGG - chr4_1:66938844-66938863 None:intergenic 35.0%
!! ACGTACTATATTGGCTTGAA+AGG + chr4_1:66938962-66938981 Msa0566020:intron 35.0%
AGGGTAAGAGAAAATCTACT+TGG - chr4_1:66939008-66939027 None:intergenic 35.0%
ATAAATGGATCAAGTGAGAC+AGG - chr4_1:66939033-66939052 None:intergenic 35.0%
ACTTATGCATCAATTTGGGA+GGG - chr4_1:66939259-66939278 None:intergenic 35.0%
AACTTATGCATCAATTTGGG+AGG - chr4_1:66939260-66939279 None:intergenic 35.0%
AGAAAGACTCTAATTCTAGC+AGG - chr4_1:66939297-66939316 None:intergenic 35.0%
GAGGATAGTACAATAGTTCA+AGG - chr4_1:66939327-66939346 None:intergenic 35.0%
! ATCTAGAAGAGTTGATTAGG+AGG - chr4_1:66939352-66939371 None:intergenic 35.0%
TCCTAATCAACTCTTCTAGA+TGG + chr4_1:66939351-66939370 Msa0566020:intron 35.0%
CCTAATCAACTCTTCTAGAT+GGG + chr4_1:66939352-66939371 Msa0566020:intron 35.0%
CTAATCAACTCTTCTAGATG+GGG + chr4_1:66939353-66939372 Msa0566020:intron 35.0%
GAAGGGAATCAAAATTAAGG+CGG - chr4_1:66939506-66939525 None:intergenic 35.0%
AGGGAAGGGAATCAAAATTA+AGG - chr4_1:66939509-66939528 None:intergenic 35.0%
!! CTCTGAAAACTACTGTTTTG+GGG - chr4_1:66939567-66939586 None:intergenic 35.0%
!! CACTCTGAAAACTACTGTTT+TGG - chr4_1:66939569-66939588 None:intergenic 35.0%
! CAGAGTGTCTGTAGATAATA+AGG + chr4_1:66939582-66939601 Msa0566020:intron 35.0%
CACACCTATATGTGATAACA+AGG - chr4_1:66939628-66939647 None:intergenic 35.0%
! CTTGTTATCACATATAGGTG+TGG + chr4_1:66939626-66939645 Msa0566020:intron 35.0%
AGGTGTGGAACCTAATAAAT+TGG + chr4_1:66939641-66939660 Msa0566020:intron 35.0%
! TTCTGAAGAATAGGCTTTAG+TGG + chr4_1:66939756-66939775 Msa0566020:intron 35.0%
! TCTGAAGAATAGGCTTTAGT+GGG + chr4_1:66939757-66939776 Msa0566020:intron 35.0%
GATTTCTACACTCATTCTCA+AGG - chr4_1:66937666-66937685 None:intergenic 35.0%
ACAATGATTCAGCTACCTAT+AGG - chr4_1:66938420-66938439 None:intergenic 35.0%
AGCTGAATCATTGTATTAGC+AGG + chr4_1:66938426-66938445 Msa0566020:intron 35.0%
!! TGCTAGGTTTTGATCCATTT+TGG + chr4_1:66938449-66938468 Msa0566020:intron 35.0%
! ATTGGTCTACTTGGTTAGAT+GGG + chr4_1:66938494-66938513 Msa0566020:intron 35.0%
!!! CTTAATTAGGGTTTTGTTCG+TGG - chr4_1:66938844-66938863 None:intergenic 35.0%
!! ACGTACTATATTGGCTTGAA+AGG + chr4_1:66938962-66938981 Msa0566020:intron 35.0%
AGGGTAAGAGAAAATCTACT+TGG - chr4_1:66939008-66939027 None:intergenic 35.0%
ATAAATGGATCAAGTGAGAC+AGG - chr4_1:66939033-66939052 None:intergenic 35.0%
ACTTATGCATCAATTTGGGA+GGG - chr4_1:66939259-66939278 None:intergenic 35.0%
AACTTATGCATCAATTTGGG+AGG - chr4_1:66939260-66939279 None:intergenic 35.0%
AGAAAGACTCTAATTCTAGC+AGG - chr4_1:66939297-66939316 None:intergenic 35.0%
GAGGATAGTACAATAGTTCA+AGG - chr4_1:66939327-66939346 None:intergenic 35.0%
! ATCTAGAAGAGTTGATTAGG+AGG - chr4_1:66939352-66939371 None:intergenic 35.0%
TCCTAATCAACTCTTCTAGA+TGG + chr4_1:66939351-66939370 Msa0566020:intron 35.0%
CCTAATCAACTCTTCTAGAT+GGG + chr4_1:66939352-66939371 Msa0566020:intron 35.0%
CTAATCAACTCTTCTAGATG+GGG + chr4_1:66939353-66939372 Msa0566020:intron 35.0%
GAAGGGAATCAAAATTAAGG+CGG - chr4_1:66939506-66939525 None:intergenic 35.0%
AGGGAAGGGAATCAAAATTA+AGG - chr4_1:66939509-66939528 None:intergenic 35.0%
!! CTCTGAAAACTACTGTTTTG+GGG - chr4_1:66939567-66939586 None:intergenic 35.0%
!! CACTCTGAAAACTACTGTTT+TGG - chr4_1:66939569-66939588 None:intergenic 35.0%
! CAGAGTGTCTGTAGATAATA+AGG + chr4_1:66939582-66939601 Msa0566020:intron 35.0%
CACACCTATATGTGATAACA+AGG - chr4_1:66939628-66939647 None:intergenic 35.0%
! CTTGTTATCACATATAGGTG+TGG + chr4_1:66939626-66939645 Msa0566020:intron 35.0%
AGGTGTGGAACCTAATAAAT+TGG + chr4_1:66939641-66939660 Msa0566020:intron 35.0%
! TTCTGAAGAATAGGCTTTAG+TGG + chr4_1:66939756-66939775 Msa0566020:intron 35.0%
! TCTGAAGAATAGGCTTTAGT+GGG + chr4_1:66939757-66939776 Msa0566020:intron 35.0%
TTATCTAGGTGAGGTGAAGT+AGG + chr4_1:66937689-66937708 Msa0566020:five_prime_UTR 40.0%
! CCATTTTCTTGTCTGTCTTG+TGG - chr4_1:66937780-66937799 None:intergenic 40.0%
CCACAAGACAGACAAGAAAA+TGG + chr4_1:66937777-66937796 Msa0566020:exon 40.0%
! GAAAATGGTGTTGAACACTC+AGG + chr4_1:66937792-66937811 Msa0566020:exon 40.0%
TGTTTCCCTCTTCATCAACT+TGG + chr4_1:66937913-66937932 Msa0566020:CDS 40.0%
GAGTAGAAGGAATAAGGTTG+AGG - chr4_1:66937976-66937995 None:intergenic 40.0%
!! ACAATCTGATCTGATGGACT+TGG + chr4_1:66938336-66938355 Msa0566020:intron 40.0%
TCATTGTATTAGCAGGTGCT+AGG + chr4_1:66938433-66938452 Msa0566020:intron 40.0%
TTGGAAGTTCAAATCGGGAT+TGG + chr4_1:66938476-66938495 Msa0566020:intron 40.0%
!! GATTGGTCTACTTGGTTAGA+TGG + chr4_1:66938493-66938512 Msa0566020:intron 40.0%
!!! TAGGGTTTTGTTCGTGGAAA+CGG - chr4_1:66938838-66938857 None:intergenic 40.0%
! CATTAGGTGAGGTTAGCTAA+GGG - chr4_1:66938895-66938914 None:intergenic 40.0%
! ACATTAGGTGAGGTTAGCTA+AGG - chr4_1:66938896-66938915 None:intergenic 40.0%
!!! TTTTGGTTGTAGTGGATCTC+TGG + chr4_1:66938923-66938942 Msa0566020:intron 40.0%
!! TTTGGTTGTAGTGGATCTCT+GGG + chr4_1:66938924-66938943 Msa0566020:intron 40.0%
!! GGATTTGCTATCATTTGGGA+TGG + chr4_1:66939160-66939179 Msa0566020:intron 40.0%
! TTGCTATCATTTGGGATGGA+TGG + chr4_1:66939164-66939183 Msa0566020:intron 40.0%
! TAGAAGAGTTGATTAGGAGG+AGG - chr4_1:66939349-66939368 None:intergenic 40.0%
! CCCATCTAGAAGAGTTGATT+AGG - chr4_1:66939355-66939374 None:intergenic 40.0%
CTTCTAGATGGGGCTTATAT+TGG + chr4_1:66939363-66939382 Msa0566020:intron 40.0%
!!! GAAAACTACTGTTTTGGGGT+TGG - chr4_1:66939563-66939582 None:intergenic 40.0%
!! GATAATAAGGGTGTCTCTGT+AGG + chr4_1:66939595-66939614 Msa0566020:intron 40.0%
TTATCTAGGTGAGGTGAAGT+AGG + chr4_1:66937689-66937708 Msa0566020:five_prime_UTR 40.0%
! CCATTTTCTTGTCTGTCTTG+TGG - chr4_1:66937780-66937799 None:intergenic 40.0%
CCACAAGACAGACAAGAAAA+TGG + chr4_1:66937777-66937796 Msa0566020:exon 40.0%
! GAAAATGGTGTTGAACACTC+AGG + chr4_1:66937792-66937811 Msa0566020:exon 40.0%
TGTTTCCCTCTTCATCAACT+TGG + chr4_1:66937913-66937932 Msa0566020:CDS 40.0%
GAGTAGAAGGAATAAGGTTG+AGG - chr4_1:66937976-66937995 None:intergenic 40.0%
!! ACAATCTGATCTGATGGACT+TGG + chr4_1:66938336-66938355 Msa0566020:intron 40.0%
TCATTGTATTAGCAGGTGCT+AGG + chr4_1:66938433-66938452 Msa0566020:intron 40.0%
TTGGAAGTTCAAATCGGGAT+TGG + chr4_1:66938476-66938495 Msa0566020:intron 40.0%
!! GATTGGTCTACTTGGTTAGA+TGG + chr4_1:66938493-66938512 Msa0566020:intron 40.0%
!!! TAGGGTTTTGTTCGTGGAAA+CGG - chr4_1:66938838-66938857 None:intergenic 40.0%
! CATTAGGTGAGGTTAGCTAA+GGG - chr4_1:66938895-66938914 None:intergenic 40.0%
! ACATTAGGTGAGGTTAGCTA+AGG - chr4_1:66938896-66938915 None:intergenic 40.0%
!!! TTTTGGTTGTAGTGGATCTC+TGG + chr4_1:66938923-66938942 Msa0566020:intron 40.0%
!! TTTGGTTGTAGTGGATCTCT+GGG + chr4_1:66938924-66938943 Msa0566020:intron 40.0%
!! GGATTTGCTATCATTTGGGA+TGG + chr4_1:66939160-66939179 Msa0566020:intron 40.0%
! TTGCTATCATTTGGGATGGA+TGG + chr4_1:66939164-66939183 Msa0566020:intron 40.0%
! TAGAAGAGTTGATTAGGAGG+AGG - chr4_1:66939349-66939368 None:intergenic 40.0%
! CCCATCTAGAAGAGTTGATT+AGG - chr4_1:66939355-66939374 None:intergenic 40.0%
CTTCTAGATGGGGCTTATAT+TGG + chr4_1:66939363-66939382 Msa0566020:intron 40.0%
!!! GAAAACTACTGTTTTGGGGT+TGG - chr4_1:66939563-66939582 None:intergenic 40.0%
!! GATAATAAGGGTGTCTCTGT+AGG + chr4_1:66939595-66939614 Msa0566020:intron 40.0%
CACTCATTCTCAAGGAGTCA+AGG - chr4_1:66937658-66937677 None:intergenic 45.0%
!! GACGACCAAGTTGATGAAGA+GGG - chr4_1:66937921-66937940 None:intergenic 45.0%
!! AGACGACCAAGTTGATGAAG+AGG - chr4_1:66937922-66937941 None:intergenic 45.0%
! TCTGATGATGCTTCTGCTTC+TGG + chr4_1:66938033-66938052 Msa0566020:CDS 45.0%
!! CAAATCGGGATTGGTCTACT+TGG + chr4_1:66938485-66938504 Msa0566020:intron 45.0%
! GTGAGGTTAGCTAAGGGATA+CGG - chr4_1:66938889-66938908 None:intergenic 45.0%
! CTCTTTTGTAGGTGAAGACC+CGG + chr4_1:66939209-66939228 Msa0566020:intron 45.0%
! AAGAGTTGATTAGGAGGAGG+AGG - chr4_1:66939346-66939365 None:intergenic 45.0%
AATGATGGCAACCGACAAAC+AGG - chr4_1:66939540-66939559 None:intergenic 45.0%
!!! CTGTTTTGGGGTTGGAATGA+TGG - chr4_1:66939555-66939574 None:intergenic 45.0%
! AATAGGCTTTAGTGGGATGC+TGG + chr4_1:66939764-66939783 Msa0566020:intron 45.0%
! ATAGGCTTTAGTGGGATGCT+GGG + chr4_1:66939765-66939784 Msa0566020:intron 45.0%
CACTCATTCTCAAGGAGTCA+AGG - chr4_1:66937658-66937677 None:intergenic 45.0%
!! GACGACCAAGTTGATGAAGA+GGG - chr4_1:66937921-66937940 None:intergenic 45.0%
!! AGACGACCAAGTTGATGAAG+AGG - chr4_1:66937922-66937941 None:intergenic 45.0%
! TCTGATGATGCTTCTGCTTC+TGG + chr4_1:66938033-66938052 Msa0566020:CDS 45.0%
!! CAAATCGGGATTGGTCTACT+TGG + chr4_1:66938485-66938504 Msa0566020:intron 45.0%
! GTGAGGTTAGCTAAGGGATA+CGG - chr4_1:66938889-66938908 None:intergenic 45.0%
! CTCTTTTGTAGGTGAAGACC+CGG + chr4_1:66939209-66939228 Msa0566020:intron 45.0%
! AAGAGTTGATTAGGAGGAGG+AGG - chr4_1:66939346-66939365 None:intergenic 45.0%
AATGATGGCAACCGACAAAC+AGG - chr4_1:66939540-66939559 None:intergenic 45.0%
!!! CTGTTTTGGGGTTGGAATGA+TGG - chr4_1:66939555-66939574 None:intergenic 45.0%
! AATAGGCTTTAGTGGGATGC+TGG + chr4_1:66939764-66939783 Msa0566020:intron 45.0%
! ATAGGCTTTAGTGGGATGCT+GGG + chr4_1:66939765-66939784 Msa0566020:intron 45.0%
GAGGTGAAGTAGGTAGCAGA+AGG + chr4_1:66937699-66937718 Msa0566020:five_prime_UTR 50.0%
TGTCTGCACAAACGTGGCAA+TGG + chr4_1:66937836-66937855 Msa0566020:CDS 50.0%
GTTAGCTAAGGGATACGGTC+TGG - chr4_1:66938884-66938903 None:intergenic 50.0%
GGATCAAGTGAGACAGGAGA+GGG - chr4_1:66939027-66939046 None:intergenic 50.0%
TGGATCAAGTGAGACAGGAG+AGG - chr4_1:66939028-66939047 None:intergenic 50.0%
AACAGGCAGCTAAGAGGGAA+GGG - chr4_1:66939523-66939542 None:intergenic 50.0%
AAACAGGCAGCTAAGAGGGA+AGG - chr4_1:66939524-66939543 None:intergenic 50.0%
CGACAAACAGGCAGCTAAGA+GGG - chr4_1:66939528-66939547 None:intergenic 50.0%
CCTCTTAGCTGCCTGTTTGT+CGG + chr4_1:66939526-66939545 Msa0566020:intron 50.0%
TTTAGTGGGATGCTGGGATC+TGG + chr4_1:66939771-66939790 Msa0566020:intron 50.0%
GAGGTGAAGTAGGTAGCAGA+AGG + chr4_1:66937699-66937718 Msa0566020:five_prime_UTR 50.0%
TGTCTGCACAAACGTGGCAA+TGG + chr4_1:66937836-66937855 Msa0566020:CDS 50.0%
GTTAGCTAAGGGATACGGTC+TGG - chr4_1:66938884-66938903 None:intergenic 50.0%
GGATCAAGTGAGACAGGAGA+GGG - chr4_1:66939027-66939046 None:intergenic 50.0%
TGGATCAAGTGAGACAGGAG+AGG - chr4_1:66939028-66939047 None:intergenic 50.0%
AACAGGCAGCTAAGAGGGAA+GGG - chr4_1:66939523-66939542 None:intergenic 50.0%
AAACAGGCAGCTAAGAGGGA+AGG - chr4_1:66939524-66939543 None:intergenic 50.0%
CGACAAACAGGCAGCTAAGA+GGG - chr4_1:66939528-66939547 None:intergenic 50.0%
CCTCTTAGCTGCCTGTTTGT+CGG + chr4_1:66939526-66939545 Msa0566020:intron 50.0%
TTTAGTGGGATGCTGGGATC+TGG + chr4_1:66939771-66939790 Msa0566020:intron 50.0%
CACGTGTGTCTGCACAAACG+TGG + chr4_1:66937830-66937849 Msa0566020:CDS 55.0%
CCGACAAACAGGCAGCTAAG+AGG - chr4_1:66939529-66939548 None:intergenic 55.0%
CACGTGTGTCTGCACAAACG+TGG + chr4_1:66937830-66937849 Msa0566020:CDS 55.0%
CCGACAAACAGGCAGCTAAG+AGG - chr4_1:66939529-66939548 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 66937650 66939882 66937650 ID=Msa0566020;Name=Msa0566020
chr4_1 mRNA 66937650 66939882 66937650 ID=Msa0566020-mRNA-1;Parent=Msa0566020;Name=Msa0566020-mRNA-1;_AED=0.27;_eAED=0.27;_QI=146|0|0.5|0.5|1|1|2|48|106
chr4_1 exon 66937650 66938114 66937650 ID=Msa0566020-mRNA-1:exon:21841;Parent=Msa0566020-mRNA-1
chr4_1 exon 66939833 66939882 66939833 ID=Msa0566020-mRNA-1:exon:21842;Parent=Msa0566020-mRNA-1
chr4_1 five_prime_UTR 66937650 66937795 66937650 ID=Msa0566020-mRNA-1:five_prime_utr;Parent=Msa0566020-mRNA-1
chr4_1 CDS 66937796 66938114 66937796 ID=Msa0566020-mRNA-1:cds;Parent=Msa0566020-mRNA-1
chr4_1 CDS 66939833 66939834 66939833 ID=Msa0566020-mRNA-1:cds;Parent=Msa0566020-mRNA-1
chr4_1 three_prime_UTR 66939835 66939882 66939835 ID=Msa0566020-mRNA-1:three_prime_utr;Parent=Msa0566020-mRNA-1
Gene Sequence

>Msa0566020

ATGGTGTTGAACACTCAGGTTGTTGTTCGAGTTGCACGTGTGTCTGCACAAACGTGGCAATGGTTGTCGTGCATTCATGATCCAATAAACAGTGATCAGCTTTTGGATCTTCTTTTTTGTTTCCCTCTTCATCAACTTGGTCGTCTTACTCTTTGTCTTTGTTCTTTCTTCTGTCTTCCTCAACCTTATTCCTTCTACTCTTCTTATCTTCTTTCTGATTCTGAGTTTGATTCTGATTCTGATGATGCTTCTGCTTCTGGTTCTTCAACTTCTACTCTTCATCTTGAACATGATTATTATTATCATTCTCATTCTGATTAA
Protein sequence

>Msa0566020

MVLNTQVVVRVARVSAQTWQWLSCIHDPINSDQLLDLLFCFPLHQLGRLTLCLCSFFCLPQPYSFYSSYLLSDSEFDSDSDDASASGSSTSTLHLEHDYYYHSHSD*