AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072242

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072242.t1 MTR_8g014210 67.111 225 73 1 259 483 8 231 2.08e-92 280
MS.gene072242.t1 MTR_8g014210 72.024 168 47 0 1 168 1 168 1.74e-72 229
MS.gene072242.t1 MTR_8g014210 61.111 162 62 1 133 294 8 168 4.53e-51 173
MS.gene072242.t1 MTR_8g014260 62.343 239 87 3 246 483 215 451 7.31e-81 258
MS.gene072242.t1 MTR_8g014260 56.548 168 73 0 1 168 220 387 2.13e-50 178
MS.gene072242.t1 MTR_8g014260 49.714 175 86 2 120 294 215 387 3.55e-37 142
MS.gene072242.t1 MTR_4g024710 60.000 225 90 0 259 483 123 347 3.63e-80 253
MS.gene072242.t1 MTR_4g024710 59.763 169 67 1 1 168 116 284 1.32e-52 181
MS.gene072242.t1 MTR_4g024710 51.852 162 78 0 133 294 123 284 3.13e-40 148
MS.gene072242.t1 MTR_8g445780 69.048 168 52 0 1 168 206 373 3.88e-71 231
MS.gene072242.t1 MTR_8g445780 69.427 157 48 0 281 437 234 390 1.37e-65 217
MS.gene072242.t1 MTR_8g445780 65.000 140 49 0 155 294 234 373 3.02e-51 179
MS.gene072242.t1 MTR_4g024745 59.677 186 75 0 281 466 99 284 2.34e-61 202
MS.gene072242.t1 MTR_4g024745 57.988 169 70 1 1 168 70 238 4.61e-50 172
MS.gene072242.t1 MTR_4g024745 53.571 140 65 0 155 294 99 238 3.38e-34 130
MS.gene072242.t1 MTR_8g077460 68.276 145 46 0 339 483 231 375 2.46e-58 197
MS.gene072242.t1 MTR_8g077460 38.750 160 77 3 8 167 173 311 3.53e-20 92.4
MS.gene072242.t1 MTR_8g077460 53.086 81 38 0 213 293 231 311 1.03e-18 88.2
MS.gene072242.t1 MTR_2g083840 39.077 325 157 8 187 483 146 457 5.73e-47 169
MS.gene072242.t1 MTR_2g083840 47.977 173 83 5 1 168 221 391 1.43e-28 118
MS.gene072242.t1 MTR_2g083840 35.135 259 130 7 61 294 146 391 2.90e-22 99.8
MS.gene072242.t1 MTR_5g092960 46.032 252 116 6 246 482 215 461 6.73e-45 164
MS.gene072242.t1 MTR_5g092960 50.000 174 72 4 9 168 229 401 1.10e-31 127
MS.gene072242.t1 MTR_5g092960 41.270 189 95 5 120 294 215 401 5.00e-20 92.8
MS.gene072242.t1 MTR_8g468810 40.636 283 127 9 236 483 187 463 2.88e-42 156
MS.gene072242.t1 MTR_8g468810 44.633 177 86 7 1 168 224 397 6.96e-26 110
MS.gene072242.t1 MTR_8g468810 35.780 218 100 9 110 294 187 397 7.51e-19 89.4
MS.gene072242.t1 MTR_8g062270 42.629 251 126 8 243 482 210 453 6.23e-40 150
MS.gene072242.t1 MTR_8g062270 53.571 140 63 2 1 139 219 357 9.70e-31 124
MS.gene072242.t1 MTR_8g062270 45.333 150 79 3 117 265 210 357 1.32e-20 94.7
MS.gene072242.t1 MTR_8g467800 41.589 214 111 5 281 483 243 453 5.12e-39 147
MS.gene072242.t1 MTR_8g467800 43.678 174 86 4 6 168 220 392 7.74e-27 113
MS.gene072242.t1 MTR_8g467800 39.735 151 79 4 155 294 243 392 5.88e-19 89.7
MS.gene072242.t1 MTR_8g058970 47.005 217 99 7 281 483 263 477 7.04e-39 147
MS.gene072242.t1 MTR_8g058970 45.251 179 85 6 1 168 235 411 9.67e-25 107
MS.gene072242.t1 MTR_8g058970 42.384 151 74 6 155 294 263 411 5.03e-14 74.7
MS.gene072242.t1 MTR_8g062310 40.800 250 131 7 247 483 183 428 1.07e-38 146
MS.gene072242.t1 MTR_8g062310 33.871 186 107 6 121 294 183 364 8.32e-12 67.4
MS.gene072242.t1 MTR_8g469020 40.684 263 122 10 249 483 195 451 6.84e-37 141
MS.gene072242.t1 MTR_8g469020 34.673 199 97 10 123 294 195 387 5.91e-11 64.7
MS.gene072242.t1 MTR_8g062080 36.972 284 143 9 224 483 174 445 1.86e-36 140
MS.gene072242.t1 MTR_8g468840 44.928 207 104 6 281 480 244 447 2.21e-35 137
MS.gene072242.t1 MTR_8g468840 46.409 181 88 5 6 179 221 399 1.35e-27 115
MS.gene072242.t1 MTR_8g468840 43.038 158 81 5 155 305 244 399 5.92e-18 86.7
MS.gene072242.t1 MTR_8g070950 38.519 270 138 9 236 483 171 434 4.67e-33 130
MS.gene072242.t1 MTR_8g062010 44.086 186 99 4 302 483 246 430 5.96e-33 130
MS.gene072242.t1 MTR_4g032730 37.456 283 129 8 244 482 188 466 1.55e-31 126
MS.gene072242.t1 MTR_4g032730 48.201 139 61 5 10 145 227 357 2.55e-19 90.9
MS.gene072242.t1 MTR_4g032730 38.506 174 83 6 118 271 188 357 1.12e-12 70.5
MS.gene072242.t1 MTR_5g092960 54.861 144 56 2 9 144 229 371 4.60e-31 124
MS.gene072242.t1 MTR_5g092960 44.025 159 79 3 120 270 215 371 2.54e-19 90.1
MS.gene072242.t1 MTR_5g092960 44.025 159 79 3 246 396 215 371 7.28e-19 89.0
MS.gene072242.t1 MTR_8g058965 38.023 263 132 9 248 483 198 456 3.00e-30 123
MS.gene072242.t1 MTR_8g014160 52.756 127 57 2 359 483 300 425 6.17e-29 118
MS.gene072242.t1 MTR_8g014230 59.140 93 37 1 391 483 13 104 1.21e-28 109
MS.gene072242.t1 MTR_8g062300 33.220 295 155 9 224 483 190 477 2.11e-25 109
MS.gene072242.t1 MTR_2g086020 46.479 142 72 3 345 483 175 315 3.62e-25 105
MS.gene072242.t1 MTR_8g468760 37.247 247 110 9 246 482 207 418 5.23e-25 107
MS.gene072242.t1 MTR_8g468760 37.647 170 68 5 6 168 217 355 8.34e-14 73.6
MS.gene072242.t1 MTR_8g468810 47.333 150 71 5 1 144 224 371 3.07e-24 104
MS.gene072242.t1 MTR_8g468810 37.173 191 84 7 236 396 187 371 1.02e-17 85.1
MS.gene072242.t1 MTR_8g468810 36.649 191 85 7 110 270 187 371 3.93e-17 83.2
MS.gene072242.t1 MTR_4g050250 36.150 213 100 8 249 429 211 419 5.50e-16 80.5
MS.gene072242.t1 MTR_8g468810 45.794 107 53 2 1 107 224 325 1.06e-14 75.5
MS.gene072242.t1 MTR_1g041245 36.885 122 71 2 368 483 265 386 2.14e-14 75.1
MS.gene072242.t1 MTR_8g468810 50.575 87 42 1 1 87 224 309 6.39e-14 73.2
MS.gene072242.t1 MTR_8g468810 50.575 87 42 1 1 87 224 309 1.01e-13 72.8
MS.gene072242.t1 MTR_3g115430 38.532 109 62 3 377 483 20 125 1.09e-13 68.2
MS.gene072242.t1 MTR_5g084070 28.916 249 152 7 250 475 173 419 1.26e-13 73.2
MS.gene072242.t1 MTR_7g032380 34.545 110 69 1 377 483 247 356 3.45e-13 71.2
MS.gene072242.t1 MTR_8g467800 51.111 90 42 2 6 93 220 309 5.56e-13 70.5
MS.gene072242.t1 MTR_7g032370 34.545 110 69 1 377 483 243 352 6.39e-13 70.5
MS.gene072242.t1 MTR_7g098510 34.921 126 75 4 365 483 266 391 3.75e-12 68.2
MS.gene072242.t1 MTR_5g080710 27.907 258 160 7 243 476 192 447 1.49e-11 66.6
MS.gene072242.t1 MTR_3g108560 28.992 238 129 7 254 482 182 388 8.73e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072242.t1 AT4G26340 28.309 272 154 7 242 483 159 419 1.54e-17 85.1
MS.gene072242.t1 AT5G38570 29.902 204 129 7 281 482 184 375 8.33e-13 70.5
MS.gene072242.t1 AT3G26920 28.622 283 158 11 233 482 68 339 4.88e-12 67.8
MS.gene072242.t1 AT3G26920 28.622 283 158 11 233 482 68 339 4.88e-12 67.8
MS.gene072242.t1 AT4G26350 28.856 201 128 5 292 483 234 428 8.06e-12 67.4
MS.gene072242.t1 AT3G49030 27.304 293 170 11 221 482 160 440 1.34e-11 67.0
MS.gene072242.t1 AT5G56820 29.355 310 163 13 188 480 153 423 5.96e-11 64.7
MS.gene072242.t1 AT1G32375 28.638 213 125 8 281 483 227 422 7.34e-11 64.3
MS.gene072242.t1 AT1G32375 28.638 213 125 8 281 483 227 422 7.34e-11 64.3

Find 118 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTCCTCAATGCTCTTTC+TGG 0.218757 8.3:-74509708 MS.gene072242:CDS
ATTCAATTTGAAGGATTTCT+AGG 0.234699 8.3:-74507507 MS.gene072242:CDS
CTTGAGTACCTTGACATTAC+TGG 0.243683 8.3:-74509834 MS.gene072242:CDS
GATGTCTTTCCTCATTTGTT+TGG 0.259465 8.3:-74508986 MS.gene072242:CDS
AACCTCCTCAATGCTCTTTC+GGG 0.261133 8.3:-74508179 MS.gene072242:CDS
AACCTCCTCAATGCTCTTTC+GGG 0.261133 8.3:-74508944 MS.gene072242:CDS
CAACCTCCTCAATGCTCTTT+CGG 0.283949 8.3:-74508180 MS.gene072242:CDS
CAACCTCCTCAATGCTCTTT+CGG 0.283949 8.3:-74508945 MS.gene072242:CDS
CATTGAGGAGGTTGTTTAAA+GGG 0.295438 8.3:+74509718 None:intergenic
GCATTGAGGAGGTTGTTTAA+AGG 0.300492 8.3:+74509717 None:intergenic
AACTTTGCCGAATGTGATTC+CGG 0.333930 8.3:+74508286 None:intergenic
AACTTTGCCGAATGTGATTC+CGG 0.333930 8.3:+74509051 None:intergenic
GCCTAGATCTTACTCTTGAT+TGG 0.350837 8.3:-74507859 MS.gene072242:CDS
GCCTAGATCTTACTCTTGAT+TGG 0.350837 8.3:-74508624 MS.gene072242:CDS
GCCTAGATCTTACTCTTGAT+TGG 0.350837 8.3:-74509389 MS.gene072242:CDS
CTGACCTTTATTCAATTTGA+AGG 0.365624 8.3:-74507516 MS.gene072242:CDS
GACTATTTCTGATACTTCCT+TGG 0.379986 8.3:-74507421 MS.gene072242:CDS
CATTGAGGAGGTTGTGTAAT+GGG 0.382101 8.3:+74508189 None:intergenic
CATTGAGGAGGTTGTGTAAT+GGG 0.382101 8.3:+74508954 None:intergenic
GTGTTCCACCTTCTTTGAAA+TGG 0.384122 8.3:-74509914 MS.gene072242:CDS
AGGTGGGATACAAGACAATT+AGG 0.388217 8.3:+74507537 None:intergenic
GATTCGGCGCGTTTATTTCA+AGG 0.390692 8.3:+74509856 None:intergenic
GTACCTAATCTTAACATTAC+CGG 0.404267 8.3:-74508305 MS.gene072242:CDS
GTACCTAATCTTAACATTAC+CGG 0.404267 8.3:-74509070 MS.gene072242:CDS
TCAAATGTGCCAAGGGCCTC+TGG 0.422983 8.3:-74507363 MS.gene072242:CDS
ATTCCGGTAATGTTAAGATT+AGG 0.439600 8.3:+74508302 None:intergenic
ATTCCGGTAATGTTAAGATT+AGG 0.439600 8.3:+74509067 None:intergenic
AACATTACCGGAATCACATT+CGG 0.442917 8.3:-74508293 MS.gene072242:CDS
AACATTACCGGAATCACATT+CGG 0.442917 8.3:-74509058 MS.gene072242:CDS
ATCGACATTGAGAATGAAGT+TGG 0.444727 8.3:-74509885 MS.gene072242:CDS
ACCTCCTCAATGCTCTTTCT+GGG 0.447483 8.3:-74509707 MS.gene072242:CDS
GATGTCTTTCCTCATTCGCT+TGG 0.452390 8.3:-74509750 MS.gene072242:CDS
AAGCAAGTTCAGTAGAGAAT+CGG 0.455880 8.3:+74507829 None:intergenic
AAGCAAGTTCAGTAGAGAAT+CGG 0.455880 8.3:+74508594 None:intergenic
AAGCAAGTTCAGTAGAGAAT+CGG 0.455880 8.3:+74509359 None:intergenic
AAATCCTTCAAATTGAATAA+AGG 0.461660 8.3:+74507512 None:intergenic
TAGGAACAGTCGTCTTTACT+AGG 0.466195 8.3:-74507582 MS.gene072242:CDS
TAGGAACAGTCGTCTTTACT+AGG 0.466195 8.3:-74508347 MS.gene072242:CDS
TAGGAACAGTCGTCTTTACT+AGG 0.466195 8.3:-74509112 MS.gene072242:CDS
GAATGTCGTACTTCTTACTT+AGG 0.473059 8.3:+74507397 None:intergenic
GTTGGGTCGTTCAACAACTA+AGG 0.473654 8.3:-74509670 MS.gene072242:intron
TCGACATTGAGAATGAAGTT+GGG 0.479574 8.3:-74509884 MS.gene072242:CDS
GGGATAAAACATCTAGTGTT+GGG 0.485053 8.3:-74509687 MS.gene072242:CDS
ATTGTCAACCATTTCAAAGA+AGG 0.487542 8.3:+74509906 None:intergenic
ACCAATCAAGAGTAAGATCT+AGG 0.488277 8.3:+74507858 None:intergenic
ACCAATCAAGAGTAAGATCT+AGG 0.488277 8.3:+74508623 None:intergenic
ACCAATCAAGAGTAAGATCT+AGG 0.488277 8.3:+74509388 None:intergenic
TTCAATTTGAAGGATTTCTA+GGG 0.491517 8.3:-74507506 MS.gene072242:CDS
ATGTCAAGGTACTCAAGATT+CGG 0.492208 8.3:+74509840 None:intergenic
TGGGATAAAACATCTAGTGT+TGG 0.495729 8.3:-74509688 MS.gene072242:CDS
CTTCAAATTGAATAAAGGTC+AGG 0.496357 8.3:+74507517 None:intergenic
GGGATAAAACATCTAGTGTT+GGG 0.498310 8.3:-74508158 MS.gene072242:CDS
GGGATAAAACATCTAGTGTT+GGG 0.498310 8.3:-74508923 MS.gene072242:CDS
TCAACAAATGACACCTCATC+TGG 0.509197 8.3:+74507480 None:intergenic
ATCTTGAAAAGTTAGATCAC+GGG 0.511342 8.3:+74508663 None:intergenic
ATCTTGAAAAGTTAGATCAC+GGG 0.511342 8.3:+74509428 None:intergenic
AGATTAGGTACAGATGGCGT+TGG 0.516836 8.3:+74508317 None:intergenic
AGATTAGGTACAGATGGCGT+TGG 0.516836 8.3:+74509082 None:intergenic
GGGGATAAAACATCTAGTGT+TGG 0.517002 8.3:-74508159 MS.gene072242:CDS
GGGGATAAAACATCTAGTGT+TGG 0.517002 8.3:-74508924 MS.gene072242:CDS
GCATTGAGGAGGTTGTGTAA+TGG 0.519233 8.3:+74508188 None:intergenic
GCATTGAGGAGGTTGTGTAA+TGG 0.519233 8.3:+74508953 None:intergenic
CAATTAGGAACAGATGGTGT+TGG 0.520057 8.3:+74507552 None:intergenic
TGGCACATTCCAGAGGCCCT+TGG 0.521691 8.3:+74507354 None:intergenic
ATCTTGAAAAGTCAGATCAC+GGG 0.528319 8.3:+74507898 None:intergenic
GTCAACCATTTCAAAGAAGG+TGG 0.533604 8.3:+74509909 None:intergenic
ATGACGGAACCAAGCGAATG+AGG 0.544668 8.3:+74509741 None:intergenic
CAAATTGAATAAAGGTCAGG+TGG 0.551056 8.3:+74507520 None:intergenic
GATCTTGAAAAGTTAGATCA+CGG 0.566515 8.3:+74508662 None:intergenic
GATCTTGAAAAGTTAGATCA+CGG 0.566515 8.3:+74509427 None:intergenic
ATTATGAGAACTTGAAGCAT+AGG 0.569230 8.3:+74507797 None:intergenic
ATTATGAGAACTTGAAGCAT+AGG 0.569230 8.3:+74508562 None:intergenic
ATTATGAGAACTTGAAGCAT+AGG 0.569230 8.3:+74509327 None:intergenic
ATGTACAAACAGTGGCTGCT+TGG 0.571852 8.3:-74507926 MS.gene072242:intron
ATGTACAAACAGTGGCTGCT+TGG 0.571852 8.3:-74508691 MS.gene072242:intron
AAAGATTATCAAATGTGCCA+AGG 0.575577 8.3:-74507371 MS.gene072242:CDS
ACAAGACAATTAGGAACAGA+TGG 0.578922 8.3:+74507546 None:intergenic
ATGTTAAGATTAGGTACAGA+TGG 0.581651 8.3:+74508311 None:intergenic
ATGTTAAGATTAGGTACAGA+TGG 0.581651 8.3:+74509076 None:intergenic
GATCTTGAAAAGTCAGATCA+CGG 0.582265 8.3:+74507897 None:intergenic
CGACATTGAGAATGAAGTTG+GGG 0.585568 8.3:-74509883 MS.gene072242:CDS
GAGTTCAGTCAAATGTAAGT+TGG 0.591511 8.3:+74507334 None:intergenic
GAGGTTGTTTAAAGGGATGA+CGG 0.592533 8.3:+74509725 None:intergenic
GTACTTCTTACTTAGGTCCA+AGG 0.592699 8.3:+74507404 None:intergenic
ATTTCTAGGGTTACCAGATG+AGG 0.601177 8.3:-74507493 MS.gene072242:CDS
GAGGTTGTGTAATGGGTTGA+CGG 0.609594 8.3:+74508196 None:intergenic
GAGGTTGTGTAATGGGTTGA+CGG 0.609594 8.3:+74508961 None:intergenic
AAGATTATCAAATGTGCCAA+GGG 0.611011 8.3:-74507370 MS.gene072242:CDS
ACAGTCGTCTTTACTAGGAT+CGG 0.611594 8.3:-74507577 MS.gene072242:CDS
TCCCCGAAAGAGCATTGAGG+AGG 0.611870 8.3:+74508177 None:intergenic
TCCCCGAAAGAGCATTGAGG+AGG 0.611870 8.3:+74508942 None:intergenic
TCCCAGAAAGAGCATTGAGG+AGG 0.611870 8.3:+74509706 None:intergenic
ATGTACAAACAGTGGCTGCT+TGG 0.612411 8.3:-74509456 MS.gene072242:intron
ACAGTCGTCTTTACTAGGAT+CGG 0.614300 8.3:-74508342 MS.gene072242:CDS
ACAGTCGTCTTTACTAGGAT+CGG 0.614300 8.3:-74509107 MS.gene072242:CDS
ATGTGATTCCAGTAATGTCA+AGG 0.622020 8.3:+74509826 None:intergenic
TTGACGGAACCAAACAAATG+AGG 0.625787 8.3:+74508977 None:intergenic
GTTGGGTCGTTCAACAACCA+AGG 0.635068 8.3:-74508141 MS.gene072242:intron
GTTGGGTCGTTCAACAACCA+AGG 0.635068 8.3:-74508906 MS.gene072242:intron
CATGTACAACTTGCATAATG+TGG 0.641750 8.3:-74509787 MS.gene072242:CDS
AAATTGAATAAAGGTCAGGT+GGG 0.648943 8.3:+74507521 None:intergenic
TTATCCCAGAAAGAGCATTG+AGG 0.649327 8.3:+74509703 None:intergenic
TTATCCCCGAAAGAGCATTG+AGG 0.651623 8.3:+74508174 None:intergenic
TTATCCCCGAAAGAGCATTG+AGG 0.651623 8.3:+74508939 None:intergenic
TCTCATAATTCAGAATGACA+AGG 0.661328 8.3:-74509313 MS.gene072242:intron
CATGTACAACTTGCATAGCG+TGG 0.663746 8.3:-74508258 MS.gene072242:CDS
CATGTACAACTTGCATAGCG+TGG 0.663746 8.3:-74509023 MS.gene072242:CDS
TCTCATAATTCAGAACGACA+AGG 0.664896 8.3:-74507783 MS.gene072242:intron
TCTCATAATTCAGAACGACA+AGG 0.664896 8.3:-74508548 MS.gene072242:intron
ACCTCCTCAATGCTCTTTCG+GGG 0.665044 8.3:-74508178 MS.gene072242:CDS
ACCTCCTCAATGCTCTTTCG+GGG 0.665044 8.3:-74508943 MS.gene072242:CDS
TCTTGAAAAGTTAGATCACG+GGG 0.670215 8.3:+74509429 None:intergenic
TCTTGAAAAGTTAGATCACG+GGG 0.676430 8.3:+74508664 None:intergenic
TGTAAGTTGGCACATTCCAG+AGG 0.679400 8.3:+74507347 None:intergenic
TCTTGAAAAGTCAGATCACG+GGG 0.701660 8.3:+74507899 None:intergenic
GTACAACTTGCATAATGTGG+TGG 0.710836 8.3:-74509784 MS.gene072242:CDS
GTACAACTTGCATAGCGTGG+TGG 0.718873 8.3:-74508255 MS.gene072242:CDS
GTACAACTTGCATAGCGTGG+TGG 0.718873 8.3:-74509020 MS.gene072242:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAATTTATTTCATAAAAAT+TGG + chr8.3:74509559-74509578 None:intergenic 10.0%
!! GTAATTTATTTCACAAAAAT+TGG + chr8.3:74508029-74508048 None:intergenic 15.0%
!!! ATAAATTGCATCTTATTTTT+TGG - chr8.3:74509586-74509605 MS.gene072242:intron 15.0%
!! AAATCCTTCAAATTGAATAA+AGG + chr8.3:74509756-74509775 None:intergenic 20.0%
!! AATTGGAAAATTTCATATCA+AGG + chr8.3:74509542-74509561 None:intergenic 20.0%
!! ACAAAATAAAACTTAAACCT+TGG + chr8.3:74508379-74508398 None:intergenic 20.0%
!! TGTTTAATTGAAGGTTAATT+TGG - chr8.3:74507977-74507996 MS.gene072242:intron 20.0%
!! TTTCACTTATATAGAGATTA+AGG - chr8.3:74507661-74507680 MS.gene072242:intron 20.0%
!!! ATAAATTGCATCTTATTTTC+TGG - chr8.3:74508056-74508075 MS.gene072242:intron 20.0%
!!! TTGTTTTATATTTGCATCAA+AGG - chr8.3:74507635-74507654 MS.gene072242:intron 20.0%
! AATTTCATATCAAGGTTATG+TGG + chr8.3:74509534-74509553 None:intergenic 25.0%
! ATTCAATTTGAAGGATTTCT+AGG - chr8.3:74509758-74509777 MS.gene072242:CDS 25.0%
! TCTCACTTATATAGAGATTA+AGG - chr8.3:74508425-74508444 MS.gene072242:intron 25.0%
! TGTTTAATCGAAGGTTAATT+TGG - chr8.3:74509507-74509526 MS.gene072242:intron 25.0%
! TTCAATTTGAAGGATTTCTA+GGG - chr8.3:74509759-74509778 MS.gene072242:CDS 25.0%
AAAGATTATCAAATGTGCCA+AGG - chr8.3:74509894-74509913 MS.gene072242:CDS 30.0%
AAATTGAATAAAGGTCAGGT+GGG + chr8.3:74509747-74509766 None:intergenic 30.0%
AAGATTATCAAATGTGCCAA+GGG - chr8.3:74509895-74509914 MS.gene072242:CDS 30.0%
AGTCATAGTTTATTCAGACT+TGG - chr8.3:74507724-74507743 MS.gene072242:intron 30.0%
AGTCATAGTTTATTGAGACT+TGG - chr8.3:74508488-74508507 MS.gene072242:intron 30.0%
ATCTTGAAAAGTTAGATCAC+GGG + chr8.3:74507840-74507859 None:intergenic 30.0%
ATGTTAAGATTAGGTACAGA+TGG + chr8.3:74508192-74508211 None:intergenic 30.0%
ATTATGAGAACTTGAAGCAT+AGG + chr8.3:74507941-74507960 None:intergenic 30.0%
ATTCAGACTTGGAAGTTATA+CGG - chr8.3:74507735-74507754 MS.gene072242:intron 30.0%
ATTCCGGTAATGTTAAGATT+AGG + chr8.3:74508201-74508220 None:intergenic 30.0%
ATTGTCAACCATTTCAAAGA+AGG + chr8.3:74507362-74507381 None:intergenic 30.0%
CTGACCTTTATTCAATTTGA+AGG - chr8.3:74509749-74509768 MS.gene072242:CDS 30.0%
CTTCAAATTGAATAAAGGTC+AGG + chr8.3:74509751-74509770 None:intergenic 30.0%
GATCTTGAAAAGTTAGATCA+CGG + chr8.3:74507841-74507860 None:intergenic 30.0%
GTACCTAATCTTAACATTAC+CGG - chr8.3:74508195-74508214 MS.gene072242:CDS 30.0%
GTCATAGTTTATTGAGACTT+GGG - chr8.3:74508489-74508508 MS.gene072242:intron 30.0%
TCTCATAATTCAGAATGACA+AGG - chr8.3:74507952-74507971 MS.gene072242:intron 30.0%
! GACAAGGTTTGTTTAATTGA+AGG - chr8.3:74507968-74507987 MS.gene072242:intron 30.0%
! GATGTCTTTTCTCATTTGTT+TGG - chr8.3:74509044-74509063 MS.gene072242:CDS 30.0%
AACATTACCGGAATCACATT+CGG - chr8.3:74508207-74508226 MS.gene072242:CDS 35.0%
AAGCAAGTTCAGTAGAGAAT+CGG + chr8.3:74507909-74507928 None:intergenic 35.0%
ACAAGACAATTAGGAACAGA+TGG + chr8.3:74509722-74509741 None:intergenic 35.0%
ACCAATCAAGAGTAAGATCT+AGG + chr8.3:74507880-74507899 None:intergenic 35.0%
ATCGACATTGAGAATGAAGT+TGG - chr8.3:74507380-74507399 MS.gene072242:CDS 35.0%
ATCTTGAAAAGTCAGATCAC+GGG + chr8.3:74509370-74509389 None:intergenic 35.0%
ATGTGATTCCAGTAATGTCA+AGG + chr8.3:74507442-74507461 None:intergenic 35.0%
CAAATTGAATAAAGGTCAGG+TGG + chr8.3:74509748-74509767 None:intergenic 35.0%
CATGTACAACTTGCATAATG+TGG - chr8.3:74507478-74507497 MS.gene072242:CDS 35.0%
CATTGAGGAGGTTGTTTAAA+GGG + chr8.3:74507550-74507569 None:intergenic 35.0%
GAATGTCGTACTTCTTACTT+AGG + chr8.3:74509871-74509890 None:intergenic 35.0%
GATCTTGAAAAGTCAGATCA+CGG + chr8.3:74509371-74509390 None:intergenic 35.0%
GATGTCATATGTACAAACAG+TGG - chr8.3:74507801-74507820 MS.gene072242:CDS 35.0%
GATGTCTTTCCTCATTTGTT+TGG - chr8.3:74508279-74508298 MS.gene072242:CDS 35.0%
GGGATAAAACATCTAGTGTT+GGG - chr8.3:74507578-74507597 MS.gene072242:CDS 35.0%
TCGACATTGAGAATGAAGTT+GGG - chr8.3:74507381-74507400 MS.gene072242:CDS 35.0%
TCTCATAATTCAGAACGACA+AGG - chr8.3:74508717-74508736 MS.gene072242:intron 35.0%
TCTTGAAAAGTTAGATCACG+GGG + chr8.3:74507839-74507858 None:intergenic 35.0%
TGGGATAAAACATCTAGTGT+TGG - chr8.3:74507577-74507596 MS.gene072242:CDS 35.0%
! ATGTCAAGGTACTCAAGATT+CGG + chr8.3:74507428-74507447 None:intergenic 35.0%
! GACAAGGTTTGTTTAATCGA+AGG - chr8.3:74509498-74509517 MS.gene072242:intron 35.0%
! GTTGAGCATGTTTTGCAAAA+CGG - chr8.3:74509803-74509822 MS.gene072242:CDS 35.0%
!! GACTATTTCTGATACTTCCT+TGG - chr8.3:74509844-74509863 MS.gene072242:CDS 35.0%
AACTTTGCCGAATGTGATTC+CGG + chr8.3:74508217-74508236 None:intergenic 40.0%
ACAGTCGTCTTTACTAGGAT+CGG - chr8.3:74508158-74508177 MS.gene072242:CDS 40.0%
AGGTGGGATACAAGACAATT+AGG + chr8.3:74509731-74509750 None:intergenic 40.0%
ATTGAGACTTGGGAGTTATG+CGG - chr8.3:74508499-74508518 MS.gene072242:intron 40.0%
CAATTAGGAACAGATGGTGT+TGG + chr8.3:74509716-74509735 None:intergenic 40.0%
CATTGAGGAGGTTGTGTAAT+GGG + chr8.3:74508314-74508333 None:intergenic 40.0%
CGACATTGAGAATGAAGTTG+GGG - chr8.3:74507382-74507401 MS.gene072242:CDS 40.0%
CTTGAGTACCTTGACATTAC+TGG - chr8.3:74507431-74507450 MS.gene072242:CDS 40.0%
GAGGTTGTTTAAAGGGATGA+CGG + chr8.3:74507543-74507562 None:intergenic 40.0%
GATGTCGTATGTACAAACAG+TGG - chr8.3:74508566-74508585 MS.gene072242:CDS 40.0%
GCATTGAGGAGGTTGTTTAA+AGG + chr8.3:74507551-74507570 None:intergenic 40.0%
GCCTAGATCTTACTCTTGAT+TGG - chr8.3:74507876-74507895 MS.gene072242:CDS 40.0%
GGGGATAAAACATCTAGTGT+TGG - chr8.3:74508341-74508360 MS.gene072242:CDS 40.0%
GTACAACTTGCATAATGTGG+TGG - chr8.3:74507481-74507500 MS.gene072242:CDS 40.0%
GTACTTCTTACTTAGGTCCA+AGG + chr8.3:74509864-74509883 None:intergenic 40.0%
GTCAACCATTTCAAAGAAGG+TGG + chr8.3:74507359-74507378 None:intergenic 40.0%
TAGGAACAGTCGTCTTTACT+AGG - chr8.3:74508153-74508172 MS.gene072242:CDS 40.0%
TCAACAAATGACACCTCATC+TGG + chr8.3:74509788-74509807 None:intergenic 40.0%
TCTTGAAAAGTCAGATCACG+GGG + chr8.3:74509369-74509388 None:intergenic 40.0%
TTGACGGAACCAAACAAATG+AGG + chr8.3:74508291-74508310 None:intergenic 40.0%
TTGGAAGTTATACGGTGGTA+AGG - chr8.3:74507743-74507762 MS.gene072242:intron 40.0%
! GTGTTCCACCTTCTTTGAAA+TGG - chr8.3:74507351-74507370 MS.gene072242:CDS 40.0%
! TCAAACCTTTTGATGCCTGT+AGG - chr8.3:74508134-74508153 MS.gene072242:intron 40.0%
!! ATTTCTAGGGTTACCAGATG+AGG - chr8.3:74509772-74509791 MS.gene072242:CDS 40.0%
!! TTATCCCAGAAAGAGCATTG+AGG + chr8.3:74507565-74507584 None:intergenic 40.0%
AACCTCCTCAATGCTCTTTC+GGG - chr8.3:74507557-74507576 MS.gene072242:CDS 45.0%
AACCTCCTCAATGCTCTTTC+TGG - chr8.3:74507557-74507576 MS.gene072242:CDS 45.0%
ACCTCCTCAATGCTCTTTCT+GGG - chr8.3:74507558-74507577 MS.gene072242:CDS 45.0%
AGATTAGGTACAGATGGCGT+TGG + chr8.3:74508186-74508205 None:intergenic 45.0%
ATGTACAAACAGTGGCTGCT+TGG - chr8.3:74507809-74507828 MS.gene072242:CDS 45.0%
CAACCTCCTCAATGCTCTTT+CGG - chr8.3:74507556-74507575 MS.gene072242:CDS 45.0%
CAGACTTGGAAGTTATACGG+TGG - chr8.3:74507738-74507757 MS.gene072242:intron 45.0%
CATGTACAACTTGCATAGCG+TGG - chr8.3:74508242-74508261 MS.gene072242:CDS 45.0%
CTGTTCCTACAGGCATCAAA+AGG + chr8.3:74508142-74508161 None:intergenic 45.0%
GAAGTTATACGGTGGTAAGG+AGG - chr8.3:74507746-74507765 MS.gene072242:intron 45.0%
GATGTCTTTCCTCATTCGCT+TGG - chr8.3:74507515-74507534 MS.gene072242:CDS 45.0%
GATTCGGCGCGTTTATTTCA+AGG + chr8.3:74507412-74507431 None:intergenic 45.0%
GCATTGAGGAGGTTGTGTAA+TGG + chr8.3:74508315-74508334 None:intergenic 45.0%
GGAGTTATGCGGTACTTCAT+CGG - chr8.3:74508510-74508529 MS.gene072242:intron 45.0%
GTAAAGACGACTGTTCCTAC+AGG + chr8.3:74508152-74508171 None:intergenic 45.0%
GTTGGGTCGTTCAACAACTA+AGG - chr8.3:74507595-74507614 MS.gene072242:intron 45.0%
! TCAAACCTTTTGTCGCCTGT+AGG - chr8.3:74509664-74509683 MS.gene072242:intron 45.0%
! TGTAAGTTGGCACATTCCAG+AGG + chr8.3:74509921-74509940 None:intergenic 45.0%
!! GAGGTTGTGTAATGGGTTGA+CGG + chr8.3:74508307-74508326 None:intergenic 45.0%
!! TTATCCCCGAAAGAGCATTG+AGG + chr8.3:74508329-74508348 None:intergenic 45.0%
ACCTCCTCAATGCTCTTTCG+GGG - chr8.3:74508322-74508341 MS.gene072242:CDS 50.0%
CTGTTCCTACAGGCGACAAA+AGG + chr8.3:74509672-74509691 None:intergenic 50.0%
GTACAACTTGCATAGCGTGG+TGG - chr8.3:74508245-74508264 MS.gene072242:CDS 50.0%
GTTGGGTCGTTCAACAACCA+AGG - chr8.3:74508359-74508378 MS.gene072242:intron 50.0%
! ATGACGGAACCAAGCGAATG+AGG + chr8.3:74507527-74507546 None:intergenic 50.0%
!! TCCCAGAAAGAGCATTGAGG+AGG + chr8.3:74507562-74507581 None:intergenic 50.0%
TCAAATGTGCCAAGGGCCTC+TGG - chr8.3:74509902-74509921 MS.gene072242:CDS 55.0%
!! TCCCCGAAAGAGCATTGAGG+AGG + chr8.3:74508326-74508345 None:intergenic 55.0%
TGGCACATTCCAGAGGCCCT+TGG + chr8.3:74509914-74509933 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 74507338 74509949 74507338 ID=MS.gene072242
chr8.3 mRNA 74507338 74509949 74507338 ID=MS.gene072242.t1;Parent=MS.gene072242
chr8.3 exon 74509671 74509949 74509671 ID=MS.gene072242.t1.exon1;Parent=MS.gene072242.t1
chr8.3 CDS 74509671 74509949 74509671 ID=cds.MS.gene072242.t1;Parent=MS.gene072242.t1
chr8.3 exon 74509314 74509466 74509314 ID=MS.gene072242.t1.exon2;Parent=MS.gene072242.t1
chr8.3 CDS 74509314 74509466 74509314 ID=cds.MS.gene072242.t1;Parent=MS.gene072242.t1
chr8.3 exon 74508907 74509131 74508907 ID=MS.gene072242.t1.exon3;Parent=MS.gene072242.t1
chr8.3 CDS 74508907 74509131 74508907 ID=cds.MS.gene072242.t1;Parent=MS.gene072242.t1
chr8.3 exon 74508549 74508701 74508549 ID=MS.gene072242.t1.exon4;Parent=MS.gene072242.t1
chr8.3 CDS 74508549 74508701 74508549 ID=cds.MS.gene072242.t1;Parent=MS.gene072242.t1
chr8.3 exon 74508142 74508366 74508142 ID=MS.gene072242.t1.exon5;Parent=MS.gene072242.t1
chr8.3 CDS 74508142 74508366 74508142 ID=cds.MS.gene072242.t1;Parent=MS.gene072242.t1
chr8.3 exon 74507784 74507936 74507784 ID=MS.gene072242.t1.exon6;Parent=MS.gene072242.t1
chr8.3 CDS 74507784 74507936 74507784 ID=cds.MS.gene072242.t1;Parent=MS.gene072242.t1
chr8.3 exon 74507338 74507601 74507338 ID=MS.gene072242.t1.exon7;Parent=MS.gene072242.t1
chr8.3 CDS 74507338 74507601 74507338 ID=cds.MS.gene072242.t1;Parent=MS.gene072242.t1
Gene Sequence

>MS.gene072242

ATGGATAAAGTTTGTGTTCCACCTTCTTTGAAATGGTTGACAATCGACATTGAGAATGAAGTTGGGGCTTACCTTGAAATAAACGCGCCGAATCTTGAGTACCTTGACATTACTGGAATCACATTCGACAAAGTTTTTCACATGTACAACTTGCATAATGTGGTGGAAGCACATCTTGATGTCTTTCCTCATTCGCTTGGTTCCGTCATCCCTTTAAACAACCTCCTCAATGCTCTTTCTGGGATAAAACATCTAGTGTTGGGTCGTTCAACAACTAAGGTTTAAATTTTGTTTTATTTGTTTTATATTTGCATCAAAGGTTATTTCACTTATATAGAGATTAAGTTAGAAGTATTAATTAACTACTATGTAACTAAAACTCTAATAGTCATAGTTTATTCAGACTTGGAAGTTATACGGTGGTAAGGAGGTCATATATATAGTTTTGTACTAATTAATACTTGATGTCATATGTACAAACAGTGGCTGCTTGGTGAACCCCGTGATCTAACTTTTCAAGATCTTCGCTGTTTGCGTCGCCTAGATCTTACTCTTGATTGGTTCAACTCCGATTCTCTACTGAACTTGCTTCAAAAATGTCCTATGCTTCAAGTTCTCATAATTCAGAATGACAAGGTTTGTTTAATTGAAGGTTAATTTGGTTGACATAACTTTGACATGAAATTTGCCAATTTTTGTGAAATAAATTACAATAGTTATAAATTGCATCTTATTTTCTGGCATACAACTTAATATAGAACATGTTTTACTCATTACTATAAATATTGCATGCTATTCAAACCTTTTGATGCCTGTAGGAACAGTCGTCTTTACTAGGATCGGATCCAACGCCATCTGTACCTAATCTTAACATTACCGGAATCACATTCGGCAAAGTTTTCAACATGTACAACTTGCATAGCGTGGTGGAAGCACATCTTGATGTCTTTCCTCATTTGTTTGGTTCCGTCAACCCATTACACAACCTCCTCAATGCTCTTTCGGGGATAAAACATCTAGTGTTGGGTCGTTCAACAACCAAGGTTTAAGTTTTATTTTGTTTATTTAATATTTGCATCAAAAGTTATCTCACTTATATAGAGATTAAGGTAAAAGTATTAATTAACTACTATGTAACTAAAACTCAAATAGTCATAGTTTATTGAGACTTGGGAGTTATGCGGTACTTCATCGGTTTATTTATATAGTTTTGTACTAATTAATACTTGATGTCGTATGTACAAACAGTGGCTGCTTGGTAAACCCCGTGATCTAACTTTTCAAGATCTTCGCTGTTTGCGTCGCCTAGATCTTACTCTTGATTGGTTCAACTCCGATTCTCTACTGAACTTGCTTCAAAAATGTCCTATGCTTCAAGTTCTCATAATTCAGAACGACAAGGTTTGTTTAATTGAAGGTTAATTTGGTTGACATAACTTTGACATGAAATTTGCCAATTTTTGTGAAATAAATTACAATAGTTATAAATTGCATCTTATTTTCTGGCACACAACTTAATATAGAACATGTTTTACTCATTACTATAAATATTGCATGCTATTCAAACCTTTTGATGCCTGTAGGAACAGTCGTCTTTACTAGGATCGGATCCAACGCCATCTGTACCTAATCTTAACATTACCGGAATCACATTCGGCAAAGTTTTCAACATGTACAACTTGCATAGCGTGGTGGAAGCACATCTTGATGTCTTTTCTCATTTGTTTGGTTCCGTCAACCCATTACACAACCTCCTCAATGCTCTTTCGGGGATAAAACATCTAGTGTTGGGTCGTTCAACAACCAAGGTTTAAGTTTTATTTTGTTTATTTAATATTTGCATCAAAAGTTATTTCACTTATATAGAGATTAAGGTAAAAGTATTAATTAACTACTATGTAACTAAAACTCTAATAGTCATAGTTTATTGAGACTTGGGAGTTATGCGGTACTTCATCGGTTTATTTATATAGTTTTGTACTAATTAATACTTGATGTCGTATGTACAAACAGTGGCTGCTTGGTAAACCCCGTGATCTGACTTTTCAAGATCTTCGCTGTTTGCGTCGCCTAGATCTTACTCTTGATTGGTTCAACTCCGATTCTCTACTGAACTTGCTTCAAAAATGTCCTATGCTTCAAGTTCTCATAATTCAGAACGACAAGGTTTGTTTAATCGAAGGTTAATTTGGTCCACATAACCTTGATATGAAATTTTCCAATTTTTATGAAATAAATTACAATAATTATAAATTGCATCTTATTTTTTGGTACACAACTTAATATAGAACTTGTTTTATTCATTACTATAAATATTGCATATTATTCAAACCTTTTGTCGCCTGTAGGAACAGTCGTCTTTACTAGGATCGGCTCCAACACCATCTGTTCCTAATTGTCTTGTATCCCACCTGACCTTTATTCAATTTGAAGGATTTCTAGGGTTACCAGATGAGGTGTCATTTGTTGAGCATGTTTTGCAAAACGGACTTGTTTTGAAGACATTGACTATTTCTGATACTTCCTTGGACCTAAGTAAGAAGTACGACATTCTCAAAAGATTATCAAATGTGCCAAGGGCCTCTGGAATGTGCCAACTTACATTTGACTGA
Protein sequence

>MS.gene072242.t1

MDKVCVPPSLKWLTIDIENEVGAYLEINAPNLEYLDITGITFDKVFHMYNLHNVVEAHLDVFPHSLGSVIPLNNLLNALSGIKHLVLGRSTTKWLLGEPRDLTFQDLRCLRRLDLTLDWFNSDSLLNLLQKCPMLQVLIIQNDKEQSSLLGSDPTPSVPNLNITGITFGKVFNMYNLHSVVEAHLDVFPHLFGSVNPLHNLLNALSGIKHLVLGRSTTKWLLGKPRDLTFQDLRCLRRLDLTLDWFNSDSLLNLLQKCPMLQVLIIQNDKEQSSLLGSDPTPSVPNLNITGITFGKVFNMYNLHSVVEAHLDVFSHLFGSVNPLHNLLNALSGIKHLVLGRSTTKWLLGKPRDLTFQDLRCLRRLDLTLDWFNSDSLLNLLQKCPMLQVLIIQNDKEQSSLLGSAPTPSVPNCLVSHLTFIQFEGFLGLPDEVSFVEHVLQNGLVLKTLTISDTSLDLSKKYDILKRLSNVPRASGMCQLTFD