Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074035.t1 | XP_003613641.1 | 84.9 | 199 | 29 | 1 | 1 | 198 | 1 | 199 | 1.30E-85 | 325.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074035.t1 | G7KB58 | 84.9 | 199 | 29 | 1 | 1 | 198 | 1 | 199 | 9.6e-86 | 325.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene074035 | 0.83512 | 2.02E-56 | -1.69E-46 |
| MS.gene049171 | MS.gene074035 | 0.82839 | 9.23E-55 | -1.69E-46 |
| MS.gene049217 | MS.gene074035 | 0.849538 | 3.03E-60 | -1.69E-46 |
| MS.gene049222 | MS.gene074035 | 0.857828 | 1.25E-62 | -1.69E-46 |
| MS.gene049285 | MS.gene074035 | 0.856187 | 3.81E-62 | -1.69E-46 |
| MS.gene049320 | MS.gene074035 | 0.859951 | 2.90E-63 | -1.69E-46 |
| MS.gene049369 | MS.gene074035 | 0.821768 | 3.39E-53 | -1.69E-46 |
| MS.gene049427 | MS.gene074035 | 0.845987 | 2.88E-59 | -1.69E-46 |
| MS.gene049609 | MS.gene074035 | 0.862775 | 3.99E-64 | -1.69E-46 |
| MS.gene049615 | MS.gene074035 | 0.814484 | 1.50E-51 | -1.69E-46 |
| MS.gene049633 | MS.gene074035 | 0.811385 | 7.19E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene074035 | 0.843198 | 1.62E-58 | -1.69E-46 |
| MS.gene050163 | MS.gene074035 | 0.83919 | 1.84E-57 | -1.69E-46 |
| MS.gene050165 | MS.gene074035 | 0.830634 | 2.63E-55 | -1.69E-46 |
| MS.gene050323 | MS.gene074035 | 0.807211 | 5.65E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene074035 | 0.811362 | 7.27E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene074035 | 0.818997 | 1.46E-52 | -1.69E-46 |
| MS.gene050444 | MS.gene074035 | 0.826265 | 2.98E-54 | -1.69E-46 |
| MS.gene050504 | MS.gene074035 | 0.826738 | 2.30E-54 | -1.69E-46 |
| MS.gene050518 | MS.gene074035 | 0.833453 | 5.29E-56 | -1.69E-46 |
| MS.gene050538 | MS.gene074035 | 0.848005 | 8.07E-60 | -1.69E-46 |
| MS.gene050577 | MS.gene074035 | 0.804692 | 1.91E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene074035 | 0.869783 | 2.39E-66 | -1.69E-46 |
| MS.gene050612 | MS.gene074035 | 0.835036 | 2.12E-56 | -1.69E-46 |
| MS.gene050670 | MS.gene074035 | 0.814972 | 1.17E-51 | -1.69E-46 |
| MS.gene050675 | MS.gene074035 | 0.828179 | 1.04E-54 | -1.69E-46 |
| MS.gene050686 | MS.gene074035 | 0.808757 | 2.65E-50 | -1.69E-46 |
| MS.gene050690 | MS.gene074035 | 0.802997 | 4.30E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene074035 | 0.830834 | 2.35E-55 | -1.69E-46 |
| MS.gene050747 | MS.gene074035 | 0.821969 | 3.04E-53 | -1.69E-46 |
| MS.gene050770 | MS.gene074035 | 0.801439 | 9.00E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene074035 | 0.872918 | 2.20E-67 | -1.69E-46 |
| MS.gene05080 | MS.gene074035 | 0.817841 | 2.68E-52 | -1.69E-46 |
| MS.gene05081 | MS.gene074035 | 0.801453 | 8.94E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene074035 | 0.806023 | 1.01E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene074035 | 0.803733 | 3.03E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene074035 | 0.821105 | 4.82E-53 | -1.69E-46 |
| MS.gene050986 | MS.gene074035 | 0.807038 | 6.14E-50 | -1.69E-46 |
| MS.gene05100 | MS.gene074035 | 0.811459 | 6.93E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene074035 | 0.824061 | 9.90E-54 | -1.69E-46 |
| MS.gene051228 | MS.gene074035 | 0.856178 | 3.84E-62 | -1.69E-46 |
| MS.gene051317 | MS.gene074035 | 0.813349 | 2.68E-51 | -1.69E-46 |
| MS.gene051387 | MS.gene074035 | 0.821496 | 3.92E-53 | -1.69E-46 |
| MS.gene051551 | MS.gene074035 | 0.811874 | 5.63E-51 | -1.69E-46 |
| MS.gene051617 | MS.gene074035 | 0.822101 | 2.84E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene074035 | 0.891054 | 5.64E-74 | -1.69E-46 |
| MS.gene051692 | MS.gene074035 | 0.830817 | 2.37E-55 | -1.69E-46 |
| MS.gene051694 | MS.gene074035 | 0.80769 | 4.47E-50 | -1.69E-46 |
| MS.gene051734 | MS.gene074035 | 0.812508 | 4.09E-51 | -1.69E-46 |
| MS.gene051810 | MS.gene074035 | 0.844914 | 5.63E-59 | -1.69E-46 |
| MS.gene051838 | MS.gene074035 | 0.834689 | 2.59E-56 | -1.69E-46 |
| MS.gene051858 | MS.gene074035 | 0.846924 | 1.60E-59 | -1.69E-46 |
| MS.gene051949 | MS.gene074035 | 0.81535 | 9.67E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene074035 | 0.808705 | 2.72E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene074035 | 0.819333 | 1.23E-52 | -1.69E-46 |
| MS.gene052290 | MS.gene074035 | 0.802999 | 4.30E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene074035 | 0.809237 | 2.09E-50 | -1.69E-46 |
| MS.gene052322 | MS.gene074035 | 0.817899 | 2.60E-52 | -1.69E-46 |
| MS.gene052379 | MS.gene074035 | 0.837586 | 4.76E-57 | -1.69E-46 |
| MS.gene052421 | MS.gene074035 | 0.812699 | 3.72E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene074035 | 0.816427 | 5.56E-52 | -1.69E-46 |
| MS.gene052501 | MS.gene074035 | 0.819993 | 8.68E-53 | -1.69E-46 |
| MS.gene052503 | MS.gene074035 | 0.82227 | 2.59E-53 | -1.69E-46 |
| MS.gene052505 | MS.gene074035 | 0.800683 | 1.28E-48 | -1.69E-46 |
| MS.gene052506 | MS.gene074035 | 0.829928 | 3.91E-55 | -1.69E-46 |
| MS.gene052508 | MS.gene074035 | 0.809334 | 1.99E-50 | -1.69E-46 |
| MS.gene052510 | MS.gene074035 | 0.807939 | 3.96E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene074035 | 0.828475 | 8.81E-55 | -1.69E-46 |
| MS.gene052822 | MS.gene074035 | 0.840914 | 6.52E-58 | -1.69E-46 |
| MS.gene052823 | MS.gene074035 | 0.812323 | 4.49E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene074035 | 0.814139 | 1.79E-51 | -1.69E-46 |
| MS.gene052870 | MS.gene074035 | 0.851675 | 7.60E-61 | -1.69E-46 |
| MS.gene052872 | MS.gene074035 | 0.853917 | 1.74E-61 | -1.69E-46 |
| MS.gene052916 | MS.gene074035 | 0.81235 | 4.43E-51 | -1.69E-46 |
| MS.gene053009 | MS.gene074035 | 0.822434 | 2.37E-53 | -1.69E-46 |
| MS.gene053103 | MS.gene074035 | 0.805535 | 1.27E-49 | -1.69E-46 |
| MS.gene053104 | MS.gene074035 | 0.860369 | 2.17E-63 | -1.69E-46 |
| MS.gene053107 | MS.gene074035 | 0.830374 | 3.05E-55 | -1.69E-46 |
| MS.gene053203 | MS.gene074035 | 0.813116 | 3.01E-51 | -1.69E-46 |
| MS.gene053207 | MS.gene074035 | 0.84963 | 2.86E-60 | -1.69E-46 |
| MS.gene053213 | MS.gene074035 | 0.860039 | 2.73E-63 | -1.69E-46 |
| MS.gene053286 | MS.gene074035 | 0.854277 | 1.37E-61 | -1.69E-46 |
| MS.gene053322 | MS.gene074035 | 0.824477 | 7.90E-54 | -1.69E-46 |
| MS.gene053384 | MS.gene074035 | 0.810906 | 9.13E-51 | -1.69E-46 |
| MS.gene053436 | MS.gene074035 | 0.801763 | 7.72E-49 | -1.69E-46 |
| MS.gene053449 | MS.gene074035 | 0.833641 | 4.75E-56 | -1.69E-46 |
| MS.gene053479 | MS.gene074035 | 0.805489 | 1.30E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene074035 | 0.85372 | 1.98E-61 | -1.69E-46 |
| MS.gene053632 | MS.gene074035 | 0.85588 | 4.69E-62 | -1.69E-46 |
| MS.gene053824 | MS.gene074035 | 0.832343 | 9.98E-56 | -1.69E-46 |
| MS.gene053916 | MS.gene074035 | 0.85758 | 1.48E-62 | -1.69E-46 |
| MS.gene054595 | MS.gene074035 | 0.801416 | 9.10E-49 | -1.69E-46 |
| MS.gene054600 | MS.gene074035 | 0.83836 | 3.01E-57 | -1.69E-46 |
| MS.gene054642 | MS.gene074035 | 0.876306 | 1.55E-68 | -1.69E-46 |
| MS.gene054706 | MS.gene074035 | -0.80118 | 1.02E-48 | -1.69E-46 |
| MS.gene054804 | MS.gene074035 | 0.802955 | 4.39E-49 | -1.69E-46 |
| MS.gene054884 | MS.gene074035 | -0.806088 | 9.75E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene074035 | 0.828613 | 8.16E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene074035 | 0.856248 | 3.66E-62 | -1.69E-46 |
| MS.gene055206 | MS.gene074035 | 0.855978 | 4.39E-62 | -1.69E-46 |
| MS.gene055224 | MS.gene074035 | 0.841197 | 5.50E-58 | -1.69E-46 |
| MS.gene055289 | MS.gene074035 | 0.837888 | 3.98E-57 | -1.69E-46 |
| MS.gene055362 | MS.gene074035 | 0.833767 | 4.41E-56 | -1.69E-46 |
| MS.gene055397 | MS.gene074035 | 0.852918 | 3.37E-61 | -1.69E-46 |
| MS.gene055499 | MS.gene074035 | 0.820499 | 6.64E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene074035 | 0.840111 | 1.06E-57 | -1.69E-46 |
| MS.gene055577 | MS.gene074035 | 0.871507 | 6.48E-67 | -1.69E-46 |
| MS.gene055647 | MS.gene074035 | 0.817271 | 3.60E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene074035 | 0.844865 | 5.80E-59 | -1.69E-46 |
| MS.gene055748 | MS.gene074035 | 0.815291 | 9.97E-52 | -1.69E-46 |
| MS.gene055750 | MS.gene074035 | 0.805564 | 1.26E-49 | -1.69E-46 |
| MS.gene055751 | MS.gene074035 | 0.811474 | 6.88E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene074035 | 0.884769 | 1.45E-71 | -1.69E-46 |
| MS.gene055941 | MS.gene074035 | 0.857842 | 1.24E-62 | -1.69E-46 |
| MS.gene055956 | MS.gene074035 | -0.804154 | 2.48E-49 | -1.69E-46 |
| MS.gene056083 | MS.gene074035 | 0.813881 | 2.05E-51 | -1.69E-46 |
| MS.gene056374 | MS.gene074035 | 0.871313 | 7.52E-67 | -1.69E-46 |
| MS.gene056487 | MS.gene074035 | 0.854817 | 9.56E-62 | -1.69E-46 |
| MS.gene056504 | MS.gene074035 | 0.818526 | 1.87E-52 | -1.69E-46 |
| MS.gene056511 | MS.gene074035 | 0.838697 | 2.47E-57 | -1.69E-46 |
| MS.gene056549 | MS.gene074035 | 0.806546 | 7.80E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene074035 | 0.823846 | 1.11E-53 | -1.69E-46 |
| MS.gene05664 | MS.gene074035 | 0.861394 | 1.06E-63 | -1.69E-46 |
| MS.gene056657 | MS.gene074035 | 0.869273 | 3.50E-66 | -1.69E-46 |
| MS.gene05666 | MS.gene074035 | 0.867916 | 9.62E-66 | -1.69E-46 |
| MS.gene056692 | MS.gene074035 | 0.818468 | 1.93E-52 | -1.69E-46 |
| MS.gene056897 | MS.gene074035 | 0.82577 | 3.91E-54 | -1.69E-46 |
| MS.gene056898 | MS.gene074035 | 0.800468 | 1.42E-48 | -1.69E-46 |
| MS.gene056947 | MS.gene074035 | 0.807594 | 4.68E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene074035 | 0.801888 | 7.28E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene074035 | 0.838158 | 3.39E-57 | -1.69E-46 |
| MS.gene057086 | MS.gene074035 | 0.821961 | 3.06E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene074035 | 0.803584 | 3.25E-49 | -1.69E-46 |
| MS.gene057129 | MS.gene074035 | 0.867988 | 9.12E-66 | -1.69E-46 |
| MS.gene057146 | MS.gene074035 | 0.800795 | 1.22E-48 | -1.69E-46 |
| MS.gene057246 | MS.gene074035 | 0.810875 | 9.27E-51 | -1.69E-46 |
| MS.gene057331 | MS.gene074035 | 0.870565 | 1.33E-66 | -1.69E-46 |
| MS.gene057332 | MS.gene074035 | 0.822533 | 2.25E-53 | -1.69E-46 |
| MS.gene05735 | MS.gene074035 | 0.832804 | 7.67E-56 | -1.69E-46 |
| MS.gene057416 | MS.gene074035 | 0.872123 | 4.05E-67 | -1.69E-46 |
| MS.gene057658 | MS.gene074035 | 0.837099 | 6.35E-57 | -1.69E-46 |
| MS.gene057734 | MS.gene074035 | 0.827844 | 1.25E-54 | -1.69E-46 |
| MS.gene057831 | MS.gene074035 | 0.8021 | 6.58E-49 | -1.69E-46 |
| MS.gene057856 | MS.gene074035 | 0.820655 | 6.12E-53 | -1.69E-46 |
| MS.gene057857 | MS.gene074035 | 0.808217 | 3.45E-50 | -1.69E-46 |
| MS.gene057926 | MS.gene074035 | 0.894511 | 2.30E-75 | -1.69E-46 |
| MS.gene057927 | MS.gene074035 | 0.826024 | 3.40E-54 | -1.69E-46 |
| MS.gene058006 | MS.gene074035 | 0.822221 | 2.66E-53 | -1.69E-46 |
| MS.gene058019 | MS.gene074035 | 0.845469 | 3.98E-59 | -1.69E-46 |
| MS.gene058323 | MS.gene074035 | 0.825158 | 5.46E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene074035 | 0.870468 | 1.43E-66 | -1.69E-46 |
| MS.gene058544 | MS.gene074035 | 0.826651 | 2.41E-54 | -1.69E-46 |
| MS.gene058660 | MS.gene074035 | 0.813476 | 2.51E-51 | -1.69E-46 |
| MS.gene058723 | MS.gene074035 | 0.875077 | 4.09E-68 | -1.69E-46 |
| MS.gene058773 | MS.gene074035 | 0.820651 | 6.13E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene074035 | 0.852167 | 5.51E-61 | -1.69E-46 |
| MS.gene05883 | MS.gene074035 | 0.80752 | 4.86E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene074035 | 0.813102 | 3.03E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene074035 | 0.836965 | 6.87E-57 | -1.69E-46 |
| MS.gene059029 | MS.gene074035 | 0.820198 | 7.79E-53 | -1.69E-46 |
| MS.gene05913 | MS.gene074035 | 0.830001 | 3.76E-55 | -1.69E-46 |
| MS.gene05934 | MS.gene074035 | 0.883563 | 4.04E-71 | -1.69E-46 |
| MS.gene059536 | MS.gene074035 | 0.831088 | 2.04E-55 | -1.69E-46 |
| MS.gene059677 | MS.gene074035 | 0.851716 | 7.40E-61 | -1.69E-46 |
| MS.gene059688 | MS.gene074035 | 0.80787 | 4.09E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene074035 | 0.853487 | 2.31E-61 | -1.69E-46 |
| MS.gene059756 | MS.gene074035 | 0.801779 | 7.67E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene074035 | 0.832542 | 8.91E-56 | -1.69E-46 |
| MS.gene059902 | MS.gene074035 | 0.841529 | 4.50E-58 | -1.69E-46 |
| MS.gene059960 | MS.gene074035 | 0.846556 | 2.02E-59 | -1.69E-46 |
| MS.gene060193 | MS.gene074035 | 0.838315 | 3.09E-57 | -1.69E-46 |
| MS.gene060403 | MS.gene074035 | 0.835572 | 1.55E-56 | -1.69E-46 |
| MS.gene060482 | MS.gene074035 | 0.822841 | 1.91E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene074035 | 0.838989 | 2.07E-57 | -1.69E-46 |
| MS.gene06077 | MS.gene074035 | 0.800205 | 1.61E-48 | -1.69E-46 |
| MS.gene060804 | MS.gene074035 | 0.90451 | 1.14E-79 | -1.69E-46 |
| MS.gene060818 | MS.gene074035 | 0.845433 | 4.07E-59 | -1.69E-46 |
| MS.gene060835 | MS.gene074035 | 0.851661 | 7.67E-61 | -1.69E-46 |
| MS.gene060837 | MS.gene074035 | 0.820213 | 7.72E-53 | -1.69E-46 |
| MS.gene06092 | MS.gene074035 | 0.848501 | 5.89E-60 | -1.69E-46 |
| MS.gene060982 | MS.gene074035 | 0.832835 | 7.53E-56 | -1.69E-46 |
| MS.gene061041 | MS.gene074035 | 0.870761 | 1.14E-66 | -1.69E-46 |
| MS.gene061051 | MS.gene074035 | -0.813942 | 1.98E-51 | -1.69E-46 |
| MS.gene061134 | MS.gene074035 | 0.825447 | 4.66E-54 | -1.69E-46 |
| MS.gene061150 | MS.gene074035 | 0.83878 | 2.35E-57 | -1.69E-46 |
| MS.gene061200 | MS.gene074035 | 0.844705 | 6.41E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074035.t1 | MTR_5g038970 | 93.970 | 199 | 11 | 1 | 1 | 198 | 1 | 199 | 1.72e-134 | 375 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074035.t1 | AT1G14345 | 78.417 | 139 | 28 | 2 | 61 | 198 | 59 | 196 | 1.72e-76 | 228 |
Find 69 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAGAAGAATTCTCAGGTTT+TGG | 0.237091 | 5.1:-29418997 | MS.gene074035:CDS |
| CAGGTTGTTCCTAGGTTTAT+TGG | 0.243976 | 5.1:-29419122 | MS.gene074035:CDS |
| CTAAGAGTGAGTGTTATTTC+AGG | 0.245187 | 5.1:-29419197 | MS.gene074035:CDS |
| GTGCACTTTACCTACGTTAC+AGG | 0.269722 | 5.1:-29419229 | MS.gene074035:CDS |
| TGTGGATGAGGTAGGGGTTG+TGG | 0.272767 | 5.1:+29419385 | None:intergenic |
| GTGGATGAGGTAGGGGTTGT+GGG | 0.275106 | 5.1:+29419386 | None:intergenic |
| GGTGTGGTTGTGGTGGTTGA+TGG | 0.281173 | 5.1:+29419499 | None:intergenic |
| CTGAAATAACACTCACTCTT+AGG | 0.291915 | 5.1:+29419198 | None:intergenic |
| GGGGTTGTGGGCTTGTTTGT+TGG | 0.299097 | 5.1:+29419398 | None:intergenic |
| TTTACGGTTGTGGGTGGAGG+TGG | 0.315642 | 5.1:+29419451 | None:intergenic |
| TTCCTAGGTTTATTGGTGAA+TGG | 0.317822 | 5.1:-29419115 | MS.gene074035:CDS |
| GGGGTTGGAGGGTGTGGTTG+TGG | 0.332045 | 5.1:+29419489 | None:intergenic |
| TGGTTGATGGTGGTGGTTGT+TGG | 0.333417 | 5.1:+29419512 | None:intergenic |
| GTTGGAGGGTGTGGTTGTGG+TGG | 0.352309 | 5.1:+29419492 | None:intergenic |
| TAGTGTTATCAAAGATGTTC+AGG | 0.370339 | 5.1:-29419141 | MS.gene074035:CDS |
| TGTGGTGAGTTTACGGTTGT+GGG | 0.371948 | 5.1:+29419442 | None:intergenic |
| AAGAAGATGGTTTCTGCATT+GGG | 0.372772 | 5.1:+29419344 | None:intergenic |
| TTGTGGTGAGTTTACGGTTG+TGG | 0.379941 | 5.1:+29419441 | None:intergenic |
| GTGGTTGTGTGGATGAGGTA+GGG | 0.384096 | 5.1:+29419378 | None:intergenic |
| TCCTAGGTTTATTGGTGAAT+GGG | 0.386223 | 5.1:-29419114 | MS.gene074035:CDS |
| GGTGGTTGTGTGGATGAGGT+AGG | 0.390073 | 5.1:+29419377 | None:intergenic |
| AAATAAAATCAAAAGCCTTT+AGG | 0.404905 | 5.1:+29418963 | None:intergenic |
| TGAAACTGCTGCTAGTGTTA+AGG | 0.412622 | 5.1:+29419253 | None:intergenic |
| GGAAGAGGGGTTGGAGGGTG+TGG | 0.412714 | 5.1:+29419483 | None:intergenic |
| AGTGTTATTTCAGGCTTAAC+GGG | 0.414099 | 5.1:-29419188 | MS.gene074035:CDS |
| GAAACTGCTGCTAGTGTTAA+GGG | 0.418053 | 5.1:+29419254 | None:intergenic |
| GAGTGTTATTTCAGGCTTAA+CGG | 0.418536 | 5.1:-29419189 | MS.gene074035:CDS |
| TGTGGGCTTGTTTGTTGGTG+AGG | 0.420960 | 5.1:+29419403 | None:intergenic |
| TTAGCAATTTCTCTAAACTT+AGG | 0.423972 | 5.1:+29419038 | None:intergenic |
| TAAGTATTGTTTGTCTATGA+AGG | 0.424827 | 5.1:-29419018 | MS.gene074035:CDS |
| TTACGGTTGTGGGTGGAGGT+GGG | 0.431500 | 5.1:+29419452 | None:intergenic |
| TACTCGGCTTCACTCTTGTA+TGG | 0.433021 | 5.1:-29419280 | MS.gene074035:CDS |
| GAGTTTACGGTTGTGGGTGG+AGG | 0.435485 | 5.1:+29419448 | None:intergenic |
| TTGATGGTGGTGGTTGTTGG+TGG | 0.448728 | 5.1:+29419515 | None:intergenic |
| TTGGATCAATCTGGTCCTAA+AGG | 0.450236 | 5.1:-29418978 | MS.gene074035:CDS |
| ATGGTACAAAGGTTGATCTT+AGG | 0.451687 | 5.1:-29419067 | MS.gene074035:CDS |
| GAAGAAGATGGTTTCTGCAT+TGG | 0.454026 | 5.1:+29419343 | None:intergenic |
| TGGTTGTGTGGATGAGGTAG+GGG | 0.456154 | 5.1:+29419379 | None:intergenic |
| GTTTGATGAGGAAGAGGGGT+TGG | 0.457551 | 5.1:+29419474 | None:intergenic |
| GTGGTTGTGGTGGTTGATGG+TGG | 0.462739 | 5.1:+29419502 | None:intergenic |
| AGGTGGTGGTTGTGTGGATG+AGG | 0.464374 | 5.1:+29419373 | None:intergenic |
| TGATGAGGAAGAGGGGTTGG+AGG | 0.465797 | 5.1:+29419477 | None:intergenic |
| ATTGTTATTACATTAAAAGA+TGG | 0.465933 | 5.1:-29419086 | MS.gene074035:CDS |
| GTTGTGGTGGTTGATGGTGG+TGG | 0.466674 | 5.1:+29419505 | None:intergenic |
| GGTGGGTTTGATGAGGAAGA+GGG | 0.474274 | 5.1:+29419469 | None:intergenic |
| GATGAGGAAGAGGGGTTGGA+GGG | 0.483645 | 5.1:+29419478 | None:intergenic |
| TATGAAGGAGAAGAATTCTC+AGG | 0.492519 | 5.1:-29419003 | MS.gene074035:CDS |
| GGGTGGAGGTGGGTTTGATG+AGG | 0.494426 | 5.1:+29419462 | None:intergenic |
| AAGATGTTCAGGTTGTTCCT+AGG | 0.508304 | 5.1:-29419130 | MS.gene074035:CDS |
| CTCATCACCAACCTCCTACT+CGG | 0.528547 | 5.1:-29419296 | MS.gene074035:CDS |
| AGGTGGGTTTGATGAGGAAG+AGG | 0.533100 | 5.1:+29419468 | None:intergenic |
| GGTGAGTTTACGGTTGTGGG+TGG | 0.544615 | 5.1:+29419445 | None:intergenic |
| TATTTCAGGCTTAACGGGTG+AGG | 0.547577 | 5.1:-29419183 | MS.gene074035:CDS |
| AGCTCCACCGTCGAAGAAGA+TGG | 0.550541 | 5.1:+29419331 | None:intergenic |
| TACATTAAAAGATGGTACAA+AGG | 0.555819 | 5.1:-29419078 | MS.gene074035:CDS |
| GAAACCATCTTCTTCGACGG+TGG | 0.561109 | 5.1:-29419335 | MS.gene074035:CDS |
| CGAGTAGGAGGTTGGTGATG+AGG | 0.561148 | 5.1:+29419297 | None:intergenic |
| CCTAGGTTTATTGGTGAATG+GGG | 0.564165 | 5.1:-29419113 | MS.gene074035:CDS |
| GTGGGTTTGATGAGGAAGAG+GGG | 0.565343 | 5.1:+29419470 | None:intergenic |
| CAAGAGTGAAGCCGAGTAGG+AGG | 0.565864 | 5.1:+29419285 | None:intergenic |
| TTCGACGGTGGAGCTCACTA+CGG | 0.570521 | 5.1:-29419323 | MS.gene074035:CDS |
| AGTGAAGCCGAGTAGGAGGT+TGG | 0.574847 | 5.1:+29419289 | None:intergenic |
| AATAACACTCACTCTTAGGT+TGG | 0.579943 | 5.1:+29419202 | None:intergenic |
| ATACAAGAGTGAAGCCGAGT+AGG | 0.584381 | 5.1:+29419282 | None:intergenic |
| GCAGAAACCATCTTCTTCGA+CGG | 0.614487 | 5.1:-29419338 | MS.gene074035:CDS |
| GGTTGGTGAACCTGTAACGT+AGG | 0.615503 | 5.1:+29419219 | None:intergenic |
| GTTGTTGGTGGTGATAAGAG+AGG | 0.626805 | 5.1:+29419527 | None:intergenic |
| TTGTTGGTGAGGATGAGACG+TGG | 0.634180 | 5.1:+29419414 | None:intergenic |
| CCCCATTCACCAATAAACCT+AGG | 0.651079 | 5.1:+29419113 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTTATTACATTAAAAGA+TGG | - | chr5.1:29419430-29419449 | MS.gene074035:CDS | 15.0% |
| ! | TACATTAAAAGATGGTACAA+AGG | - | chr5.1:29419438-29419457 | MS.gene074035:CDS | 25.0% |
| ! | TTAGCAATTTCTCTAAACTT+AGG | + | chr5.1:29419481-29419500 | None:intergenic | 25.0% |
| !! | TAAGTATTGTTTGTCTATGA+AGG | - | chr5.1:29419498-29419517 | MS.gene074035:CDS | 25.0% |
| TAGTGTTATCAAAGATGTTC+AGG | - | chr5.1:29419375-29419394 | MS.gene074035:CDS | 30.0% | |
| AAGAAGATGGTTTCTGCATT+GGG | + | chr5.1:29419175-29419194 | None:intergenic | 35.0% | |
| AATAACACTCACTCTTAGGT+TGG | + | chr5.1:29419317-29419336 | None:intergenic | 35.0% | |
| CTGAAATAACACTCACTCTT+AGG | + | chr5.1:29419321-29419340 | None:intergenic | 35.0% | |
| TATGAAGGAGAAGAATTCTC+AGG | - | chr5.1:29419513-29419532 | MS.gene074035:CDS | 35.0% | |
| TCCTAGGTTTATTGGTGAAT+GGG | - | chr5.1:29419402-29419421 | MS.gene074035:CDS | 35.0% | |
| TTCCTAGGTTTATTGGTGAA+TGG | - | chr5.1:29419401-29419420 | MS.gene074035:CDS | 35.0% | |
| ! | ATGGTACAAAGGTTGATCTT+AGG | - | chr5.1:29419449-29419468 | MS.gene074035:CDS | 35.0% |
| !! | AGTGTTATTTCAGGCTTAAC+GGG | - | chr5.1:29419328-29419347 | MS.gene074035:CDS | 35.0% |
| !! | CTAAGAGTGAGTGTTATTTC+AGG | - | chr5.1:29419319-29419338 | MS.gene074035:CDS | 35.0% |
| !! | GAGTGTTATTTCAGGCTTAA+CGG | - | chr5.1:29419327-29419346 | MS.gene074035:CDS | 35.0% |
| CAGGTTGTTCCTAGGTTTAT+TGG | - | chr5.1:29419394-29419413 | MS.gene074035:CDS | 40.0% | |
| CCTAGGTTTATTGGTGAATG+GGG | - | chr5.1:29419403-29419422 | MS.gene074035:CDS | 40.0% | |
| GAAACTGCTGCTAGTGTTAA+GGG | + | chr5.1:29419265-29419284 | None:intergenic | 40.0% | |
| GAAGAAGATGGTTTCTGCAT+TGG | + | chr5.1:29419176-29419195 | None:intergenic | 40.0% | |
| TGAAACTGCTGCTAGTGTTA+AGG | + | chr5.1:29419266-29419285 | None:intergenic | 40.0% | |
| TTGGATCAATCTGGTCCTAA+AGG | - | chr5.1:29419538-29419557 | MS.gene074035:CDS | 40.0% | |
| ! | GGAGAAGAATTCTCAGGTTT+TGG | - | chr5.1:29419519-29419538 | MS.gene074035:CDS | 40.0% |
| !! | AAGATGTTCAGGTTGTTCCT+AGG | - | chr5.1:29419386-29419405 | MS.gene074035:CDS | 40.0% |
| !! | GGAGTTTTGTGGTGAGTTTA+CGG | + | chr5.1:29419084-29419103 | None:intergenic | 40.0% |
| !!! | TCTCAGGTTTTGGATCAATC+TGG | - | chr5.1:29419529-29419548 | MS.gene074035:CDS | 40.0% |
| ATACAAGAGTGAAGCCGAGT+AGG | + | chr5.1:29419237-29419256 | None:intergenic | 45.0% | |
| CCCCATTCACCAATAAACCT+AGG | + | chr5.1:29419406-29419425 | None:intergenic | 45.0% | |
| GCAGAAACCATCTTCTTCGA+CGG | - | chr5.1:29419178-29419197 | MS.gene074035:CDS | 45.0% | |
| GTGCACTTTACCTACGTTAC+AGG | - | chr5.1:29419287-29419306 | MS.gene074035:CDS | 45.0% | |
| TACTCGGCTTCACTCTTGTA+TGG | - | chr5.1:29419236-29419255 | MS.gene074035:CDS | 45.0% | |
| TGTGGTGAGTTTACGGTTGT+GGG | + | chr5.1:29419077-29419096 | None:intergenic | 45.0% | |
| TTGTGGTGAGTTTACGGTTG+TGG | + | chr5.1:29419078-29419097 | None:intergenic | 45.0% | |
| ! | GTTTCTGCATTGGGCTTTTG+AGG | + | chr5.1:29419166-29419185 | None:intergenic | 45.0% |
| ! | TATTTCAGGCTTAACGGGTG+AGG | - | chr5.1:29419333-29419352 | MS.gene074035:CDS | 45.0% |
| !! | GTTGTTGGTGGTGATAAGAG+AGG | + | chr5.1:29418992-29419011 | None:intergenic | 45.0% |
| CTCATCACCAACCTCCTACT+CGG | - | chr5.1:29419220-29419239 | MS.gene074035:CDS | 50.0% | |
| GAAACCATCTTCTTCGACGG+TGG | - | chr5.1:29419181-29419200 | MS.gene074035:CDS | 50.0% | |
| GTGGGTTTGATGAGGAAGAG+GGG | + | chr5.1:29419049-29419068 | None:intergenic | 50.0% | |
| GTGGTTGTGTGGATGAGGTA+GGG | + | chr5.1:29419141-29419160 | None:intergenic | 50.0% | |
| GTTTGATGAGGAAGAGGGGT+TGG | + | chr5.1:29419045-29419064 | None:intergenic | 50.0% | |
| TGGTTGTGTGGATGAGGTAG+GGG | + | chr5.1:29419140-29419159 | None:intergenic | 50.0% | |
| ! | AGGTGGGTTTGATGAGGAAG+AGG | + | chr5.1:29419051-29419070 | None:intergenic | 50.0% |
| ! | GGATGAGACGTGGAGTTTTG+TGG | + | chr5.1:29419095-29419114 | None:intergenic | 50.0% |
| ! | GGTGGGTTTGATGAGGAAGA+GGG | + | chr5.1:29419050-29419069 | None:intergenic | 50.0% |
| ! | TGTGGGCTTGTTTGTTGGTG+AGG | + | chr5.1:29419116-29419135 | None:intergenic | 50.0% |
| ! | TTGTTGGTGAGGATGAGACG+TGG | + | chr5.1:29419105-29419124 | None:intergenic | 50.0% |
| !! | GGTTGGTGAACCTGTAACGT+AGG | + | chr5.1:29419300-29419319 | None:intergenic | 50.0% |
| !! | TCTGCATTGGGCTTTTGAGG+TGG | + | chr5.1:29419163-29419182 | None:intergenic | 50.0% |
| !! | TGGTTGATGGTGGTGGTTGT+TGG | + | chr5.1:29419007-29419026 | None:intergenic | 50.0% |
| !! | TTGATGGTGGTGGTTGTTGG+TGG | + | chr5.1:29419004-29419023 | None:intergenic | 50.0% |
| !!! | CTTTTGAGGTGGTGGTTGTG+TGG | + | chr5.1:29419152-29419171 | None:intergenic | 50.0% |
| AGCTCCACCGTCGAAGAAGA+TGG | + | chr5.1:29419188-29419207 | None:intergenic | 55.0% | |
| AGTGAAGCCGAGTAGGAGGT+TGG | + | chr5.1:29419230-29419249 | None:intergenic | 55.0% | |
| CAAGAGTGAAGCCGAGTAGG+AGG | + | chr5.1:29419234-29419253 | None:intergenic | 55.0% | |
| GAGTTTACGGTTGTGGGTGG+AGG | + | chr5.1:29419071-29419090 | None:intergenic | 55.0% | |
| GATGAGGAAGAGGGGTTGGA+GGG | + | chr5.1:29419041-29419060 | None:intergenic | 55.0% | |
| GGTGAGTTTACGGTTGTGGG+TGG | + | chr5.1:29419074-29419093 | None:intergenic | 55.0% | |
| GGTGGTTGTGTGGATGAGGT+AGG | + | chr5.1:29419142-29419161 | None:intergenic | 55.0% | |
| GGTGTGGTTGTGGTGGTTGA+TGG | + | chr5.1:29419020-29419039 | None:intergenic | 55.0% | |
| GTGGATGAGGTAGGGGTTGT+GGG | + | chr5.1:29419133-29419152 | None:intergenic | 55.0% | |
| TGATGAGGAAGAGGGGTTGG+AGG | + | chr5.1:29419042-29419061 | None:intergenic | 55.0% | |
| TGTGGATGAGGTAGGGGTTG+TGG | + | chr5.1:29419134-29419153 | None:intergenic | 55.0% | |
| TTACGGTTGTGGGTGGAGGT+GGG | + | chr5.1:29419067-29419086 | None:intergenic | 55.0% | |
| TTCGACGGTGGAGCTCACTA+CGG | - | chr5.1:29419193-29419212 | MS.gene074035:CDS | 55.0% | |
| TTTACGGTTGTGGGTGGAGG+TGG | + | chr5.1:29419068-29419087 | None:intergenic | 55.0% | |
| ! | AGGTGGTGGTTGTGTGGATG+AGG | + | chr5.1:29419146-29419165 | None:intergenic | 55.0% |
| ! | GGGGTTGTGGGCTTGTTTGT+TGG | + | chr5.1:29419121-29419140 | None:intergenic | 55.0% |
| !! | CGAGTAGGAGGTTGGTGATG+AGG | + | chr5.1:29419222-29419241 | None:intergenic | 55.0% |
| !! | GTGGTTGTGGTGGTTGATGG+TGG | + | chr5.1:29419017-29419036 | None:intergenic | 55.0% |
| !! | GTTGTGGTGGTTGATGGTGG+TGG | + | chr5.1:29419014-29419033 | None:intergenic | 55.0% |
| !!! | GCATTGGGCTTTTGAGGTGG+TGG | + | chr5.1:29419160-29419179 | None:intergenic | 55.0% |
| ! | GGGTGGAGGTGGGTTTGATG+AGG | + | chr5.1:29419057-29419076 | None:intergenic | 60.0% |
| ! | GTTGGAGGGTGTGGTTGTGG+TGG | + | chr5.1:29419027-29419046 | None:intergenic | 60.0% |
| GGAAGAGGGGTTGGAGGGTG+TGG | + | chr5.1:29419036-29419055 | None:intergenic | 65.0% | |
| ! | GGGGTTGGAGGGTGTGGTTG+TGG | + | chr5.1:29419030-29419049 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 29418971 | 29419567 | 29418971 | ID=MS.gene074035 |
| chr5.1 | mRNA | 29418971 | 29419567 | 29418971 | ID=MS.gene074035.t1;Parent=MS.gene074035 |
| chr5.1 | exon | 29418971 | 29419567 | 29418971 | ID=MS.gene074035.t1.exon1;Parent=MS.gene074035.t1 |
| chr5.1 | CDS | 29418971 | 29419567 | 29418971 | ID=cds.MS.gene074035.t1;Parent=MS.gene074035.t1 |
| Gene Sequence |
| Protein sequence |
