Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32769.t1 | RHN70500.1 | 95.8 | 120 | 5 | 0 | 1 | 120 | 24 | 143 | 3.20E-50 | 207.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32769.t1 | G7J8E2 | 95.8 | 120 | 5 | 0 | 1 | 120 | 1 | 120 | 2.3e-50 | 207.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene32769 | 0.80713 | 5.88E-50 | -1.69E-46 |
| MS.gene049171 | MS.gene32769 | 0.837305 | 5.62E-57 | -1.69E-46 |
| MS.gene049173 | MS.gene32769 | 0.84966 | 2.80E-60 | -1.69E-46 |
| MS.gene049217 | MS.gene32769 | 0.864393 | 1.26E-64 | -1.69E-46 |
| MS.gene049222 | MS.gene32769 | 0.847298 | 1.26E-59 | -1.69E-46 |
| MS.gene049285 | MS.gene32769 | 0.867636 | 1.18E-65 | -1.69E-46 |
| MS.gene049320 | MS.gene32769 | 0.894619 | 2.08E-75 | -1.69E-46 |
| MS.gene049369 | MS.gene32769 | 0.803546 | 3.31E-49 | -1.69E-46 |
| MS.gene04939 | MS.gene32769 | 0.803897 | 2.80E-49 | -1.69E-46 |
| MS.gene049427 | MS.gene32769 | 0.872386 | 3.31E-67 | -1.69E-46 |
| MS.gene049491 | MS.gene32769 | 0.813144 | 2.97E-51 | -1.69E-46 |
| MS.gene049609 | MS.gene32769 | 0.865524 | 5.55E-65 | -1.69E-46 |
| MS.gene049614 | MS.gene32769 | 0.825849 | 3.75E-54 | -1.69E-46 |
| MS.gene049633 | MS.gene32769 | 0.819076 | 1.40E-52 | -1.69E-46 |
| MS.gene049661 | MS.gene32769 | 0.827231 | 1.75E-54 | -1.69E-46 |
| MS.gene049662 | MS.gene32769 | 0.813785 | 2.15E-51 | -1.69E-46 |
| MS.gene049760 | MS.gene32769 | 0.816973 | 4.20E-52 | -1.69E-46 |
| MS.gene049805 | MS.gene32769 | 0.866178 | 3.45E-65 | -1.69E-46 |
| MS.gene050076 | MS.gene32769 | 0.851495 | 8.55E-61 | -1.69E-46 |
| MS.gene05010 | MS.gene32769 | 0.801161 | 1.03E-48 | -1.69E-46 |
| MS.gene050163 | MS.gene32769 | 0.884429 | 1.93E-71 | -1.69E-46 |
| MS.gene050165 | MS.gene32769 | 0.86612 | 3.60E-65 | -1.69E-46 |
| MS.gene050333 | MS.gene32769 | 0.850706 | 1.43E-60 | -1.69E-46 |
| MS.gene050353 | MS.gene32769 | -0.805532 | 1.28E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene32769 | 0.893493 | 5.97E-75 | -1.69E-46 |
| MS.gene050504 | MS.gene32769 | 0.844946 | 5.52E-59 | -1.69E-46 |
| MS.gene050518 | MS.gene32769 | 0.859786 | 3.25E-63 | -1.69E-46 |
| MS.gene050538 | MS.gene32769 | 0.821539 | 3.83E-53 | -1.69E-46 |
| MS.gene050593 | MS.gene32769 | 0.849696 | 2.74E-60 | -1.69E-46 |
| MS.gene050612 | MS.gene32769 | 0.871861 | 4.95E-67 | -1.69E-46 |
| MS.gene050662 | MS.gene32769 | 0.807647 | 4.56E-50 | -1.69E-46 |
| MS.gene050675 | MS.gene32769 | 0.849442 | 3.23E-60 | -1.69E-46 |
| MS.gene050686 | MS.gene32769 | 0.819825 | 9.48E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene32769 | 0.861835 | 7.76E-64 | -1.69E-46 |
| MS.gene050747 | MS.gene32769 | 0.825878 | 3.69E-54 | -1.69E-46 |
| MS.gene050767 | MS.gene32769 | 0.892042 | 2.29E-74 | -1.69E-46 |
| MS.gene050770 | MS.gene32769 | 0.891869 | 2.68E-74 | -1.69E-46 |
| MS.gene050772 | MS.gene32769 | 0.888008 | 8.64E-73 | -1.69E-46 |
| MS.gene05080 | MS.gene32769 | 0.827203 | 1.78E-54 | -1.69E-46 |
| MS.gene050950 | MS.gene32769 | 0.832854 | 7.45E-56 | -1.69E-46 |
| MS.gene050986 | MS.gene32769 | 0.854896 | 9.07E-62 | -1.69E-46 |
| MS.gene05100 | MS.gene32769 | 0.8546 | 1.10E-61 | -1.69E-46 |
| MS.gene05101 | MS.gene32769 | 0.832138 | 1.12E-55 | -1.69E-46 |
| MS.gene05105 | MS.gene32769 | 0.84676 | 1.77E-59 | -1.69E-46 |
| MS.gene051228 | MS.gene32769 | 0.887229 | 1.71E-72 | -1.69E-46 |
| MS.gene051387 | MS.gene32769 | 0.805674 | 1.19E-49 | -1.69E-46 |
| MS.gene051434 | MS.gene32769 | 0.812077 | 5.08E-51 | -1.69E-46 |
| MS.gene051551 | MS.gene32769 | 0.834838 | 2.38E-56 | -1.69E-46 |
| MS.gene051617 | MS.gene32769 | 0.820328 | 7.27E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene32769 | 0.853274 | 2.66E-61 | -1.69E-46 |
| MS.gene051692 | MS.gene32769 | 0.839622 | 1.42E-57 | -1.69E-46 |
| MS.gene051694 | MS.gene32769 | 0.805957 | 1.04E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene32769 | 0.920967 | 6.53E-88 | -1.69E-46 |
| MS.gene051858 | MS.gene32769 | 0.847752 | 9.48E-60 | -1.69E-46 |
| MS.gene051949 | MS.gene32769 | 0.804281 | 2.33E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene32769 | 0.802968 | 4.36E-49 | -1.69E-46 |
| MS.gene052271 | MS.gene32769 | 0.897762 | 1.03E-76 | -1.69E-46 |
| MS.gene052290 | MS.gene32769 | 0.839703 | 1.35E-57 | -1.69E-46 |
| MS.gene052310 | MS.gene32769 | 0.808617 | 2.84E-50 | -1.69E-46 |
| MS.gene052379 | MS.gene32769 | 0.868407 | 6.68E-66 | -1.69E-46 |
| MS.gene052421 | MS.gene32769 | 0.806229 | 9.10E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene32769 | 0.8348 | 2.43E-56 | -1.69E-46 |
| MS.gene052484 | MS.gene32769 | 0.870251 | 1.68E-66 | -1.69E-46 |
| MS.gene052615 | MS.gene32769 | 0.874643 | 5.75E-68 | -1.69E-46 |
| MS.gene052916 | MS.gene32769 | 0.842737 | 2.15E-58 | -1.69E-46 |
| MS.gene053009 | MS.gene32769 | 0.879521 | 1.16E-69 | -1.69E-46 |
| MS.gene053103 | MS.gene32769 | 0.858368 | 8.64E-63 | -1.69E-46 |
| MS.gene053104 | MS.gene32769 | 0.895075 | 1.35E-75 | -1.69E-46 |
| MS.gene053107 | MS.gene32769 | 0.811451 | 6.96E-51 | -1.69E-46 |
| MS.gene053207 | MS.gene32769 | 0.88349 | 4.29E-71 | -1.69E-46 |
| MS.gene053209 | MS.gene32769 | 0.852145 | 5.59E-61 | -1.69E-46 |
| MS.gene053213 | MS.gene32769 | 0.896813 | 2.57E-76 | -1.69E-46 |
| MS.gene053286 | MS.gene32769 | 0.890734 | 7.53E-74 | -1.69E-46 |
| MS.gene053322 | MS.gene32769 | 0.898704 | 4.09E-77 | -1.69E-46 |
| MS.gene053449 | MS.gene32769 | 0.812417 | 4.28E-51 | -1.69E-46 |
| MS.gene053561 | MS.gene32769 | -0.809871 | 1.53E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene32769 | 0.858092 | 1.04E-62 | -1.69E-46 |
| MS.gene053632 | MS.gene32769 | 0.863037 | 3.31E-64 | -1.69E-46 |
| MS.gene053813 | MS.gene32769 | 0.813961 | 1.96E-51 | -1.69E-46 |
| MS.gene053915 | MS.gene32769 | 0.805954 | 1.04E-49 | -1.69E-46 |
| MS.gene053916 | MS.gene32769 | 0.823416 | 1.40E-53 | -1.69E-46 |
| MS.gene054376 | MS.gene32769 | 0.89036 | 1.06E-73 | -1.69E-46 |
| MS.gene054408 | MS.gene32769 | 0.805667 | 1.20E-49 | -1.69E-46 |
| MS.gene054600 | MS.gene32769 | 0.866958 | 1.95E-65 | -1.69E-46 |
| MS.gene054642 | MS.gene32769 | 0.910249 | 2.31E-82 | -1.69E-46 |
| MS.gene054802 | MS.gene32769 | 0.8289 | 6.95E-55 | -1.69E-46 |
| MS.gene054803 | MS.gene32769 | 0.827807 | 1.28E-54 | -1.69E-46 |
| MS.gene054804 | MS.gene32769 | 0.832213 | 1.07E-55 | -1.69E-46 |
| MS.gene054927 | MS.gene32769 | 0.809382 | 1.95E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene32769 | 0.837986 | 3.76E-57 | -1.69E-46 |
| MS.gene05505 | MS.gene32769 | 0.835827 | 1.34E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene32769 | 0.814703 | 1.35E-51 | -1.69E-46 |
| MS.gene055154 | MS.gene32769 | -0.810786 | 9.69E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene32769 | 0.857324 | 1.76E-62 | -1.69E-46 |
| MS.gene055212 | MS.gene32769 | 0.835199 | 1.93E-56 | -1.69E-46 |
| MS.gene055254 | MS.gene32769 | 0.85674 | 2.62E-62 | -1.69E-46 |
| MS.gene055277 | MS.gene32769 | 0.823228 | 1.55E-53 | -1.69E-46 |
| MS.gene055289 | MS.gene32769 | 0.837135 | 6.21E-57 | -1.69E-46 |
| MS.gene055362 | MS.gene32769 | 0.875378 | 3.23E-68 | -1.69E-46 |
| MS.gene055397 | MS.gene32769 | 0.890834 | 6.88E-74 | -1.69E-46 |
| MS.gene055412 | MS.gene32769 | 0.82251 | 2.28E-53 | -1.69E-46 |
| MS.gene055499 | MS.gene32769 | 0.907554 | 4.47E-81 | -1.69E-46 |
| MS.gene055502 | MS.gene32769 | 0.858217 | 9.59E-63 | -1.69E-46 |
| MS.gene055577 | MS.gene32769 | 0.834755 | 2.49E-56 | -1.69E-46 |
| MS.gene055636 | MS.gene32769 | 0.845579 | 3.72E-59 | -1.69E-46 |
| MS.gene055638 | MS.gene32769 | 0.835326 | 1.79E-56 | -1.69E-46 |
| MS.gene055647 | MS.gene32769 | 0.886198 | 4.21E-72 | -1.69E-46 |
| MS.gene055722 | MS.gene32769 | 0.894552 | 2.22E-75 | -1.69E-46 |
| MS.gene05592 | MS.gene32769 | 0.901546 | 2.40E-78 | -1.69E-46 |
| MS.gene055941 | MS.gene32769 | 0.821572 | 3.76E-53 | -1.69E-46 |
| MS.gene055956 | MS.gene32769 | -0.818711 | 1.70E-52 | -1.69E-46 |
| MS.gene056083 | MS.gene32769 | 0.854721 | 1.02E-61 | -1.69E-46 |
| MS.gene056191 | MS.gene32769 | 0.809878 | 1.52E-50 | -1.69E-46 |
| MS.gene056374 | MS.gene32769 | 0.903582 | 2.99E-79 | -1.69E-46 |
| MS.gene056410 | MS.gene32769 | 0.807873 | 4.09E-50 | -1.69E-46 |
| MS.gene056422 | MS.gene32769 | 0.809247 | 2.08E-50 | -1.69E-46 |
| MS.gene056487 | MS.gene32769 | 0.804327 | 2.28E-49 | -1.69E-46 |
| MS.gene056510 | MS.gene32769 | 0.827856 | 1.24E-54 | -1.69E-46 |
| MS.gene056511 | MS.gene32769 | 0.805871 | 1.08E-49 | -1.69E-46 |
| MS.gene056549 | MS.gene32769 | 0.805715 | 1.17E-49 | -1.69E-46 |
| MS.gene056609 | MS.gene32769 | 0.845885 | 3.07E-59 | -1.69E-46 |
| MS.gene056657 | MS.gene32769 | 0.885576 | 7.22E-72 | -1.69E-46 |
| MS.gene056897 | MS.gene32769 | 0.857843 | 1.24E-62 | -1.69E-46 |
| MS.gene057011 | MS.gene32769 | 0.851145 | 1.07E-60 | -1.69E-46 |
| MS.gene057076 | MS.gene32769 | 0.844742 | 6.26E-59 | -1.69E-46 |
| MS.gene057124 | MS.gene32769 | 0.815335 | 9.75E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene32769 | 0.912142 | 2.73E-83 | -1.69E-46 |
| MS.gene057146 | MS.gene32769 | 0.837595 | 4.74E-57 | -1.69E-46 |
| MS.gene057196 | MS.gene32769 | 0.81448 | 1.51E-51 | -1.69E-46 |
| MS.gene057246 | MS.gene32769 | 0.829864 | 4.05E-55 | -1.69E-46 |
| MS.gene057295 | MS.gene32769 | 0.815845 | 7.50E-52 | -1.69E-46 |
| MS.gene057331 | MS.gene32769 | 0.890757 | 7.38E-74 | -1.69E-46 |
| MS.gene05735 | MS.gene32769 | 0.817026 | 4.08E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene32769 | 0.88572 | 6.37E-72 | -1.69E-46 |
| MS.gene057926 | MS.gene32769 | 0.816122 | 6.51E-52 | -1.69E-46 |
| MS.gene058006 | MS.gene32769 | 0.822336 | 2.50E-53 | -1.69E-46 |
| MS.gene058019 | MS.gene32769 | 0.852121 | 5.68E-61 | -1.69E-46 |
| MS.gene058130 | MS.gene32769 | 0.842955 | 1.88E-58 | -1.69E-46 |
| MS.gene058293 | MS.gene32769 | 0.81853 | 1.87E-52 | -1.69E-46 |
| MS.gene058323 | MS.gene32769 | 0.834219 | 3.40E-56 | -1.69E-46 |
| MS.gene058419 | MS.gene32769 | 0.894164 | 3.19E-75 | -1.69E-46 |
| MS.gene058544 | MS.gene32769 | 0.843907 | 1.05E-58 | -1.69E-46 |
| MS.gene058660 | MS.gene32769 | 0.840624 | 7.77E-58 | -1.69E-46 |
| MS.gene058723 | MS.gene32769 | 0.876749 | 1.09E-68 | -1.69E-46 |
| MS.gene058773 | MS.gene32769 | 0.857322 | 1.77E-62 | -1.69E-46 |
| MS.gene058838 | MS.gene32769 | 0.806197 | 9.25E-50 | -1.69E-46 |
| MS.gene05883 | MS.gene32769 | 0.825177 | 5.40E-54 | -1.69E-46 |
| MS.gene058840 | MS.gene32769 | -0.81856 | 1.84E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene32769 | 0.868319 | 7.13E-66 | -1.69E-46 |
| MS.gene059023 | MS.gene32769 | 0.805369 | 1.38E-49 | -1.69E-46 |
| MS.gene059029 | MS.gene32769 | 0.888397 | 6.13E-73 | -1.69E-46 |
| MS.gene05913 | MS.gene32769 | 0.841276 | 5.24E-58 | -1.69E-46 |
| MS.gene05934 | MS.gene32769 | 0.817965 | 2.51E-52 | -1.69E-46 |
| MS.gene059367 | MS.gene32769 | 0.802521 | 5.39E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene32769 | 0.886566 | 3.06E-72 | -1.69E-46 |
| MS.gene059677 | MS.gene32769 | 0.872483 | 3.07E-67 | -1.69E-46 |
| MS.gene059688 | MS.gene32769 | 0.845224 | 4.64E-59 | -1.69E-46 |
| MS.gene059692 | MS.gene32769 | 0.846777 | 1.75E-59 | -1.69E-46 |
| MS.gene059695 | MS.gene32769 | 0.845032 | 5.23E-59 | -1.69E-46 |
| MS.gene059711 | MS.gene32769 | 0.828618 | 8.13E-55 | -1.69E-46 |
| MS.gene059718 | MS.gene32769 | 0.873996 | 9.54E-68 | -1.69E-46 |
| MS.gene059858 | MS.gene32769 | 0.828839 | 7.19E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene32769 | 0.836457 | 9.25E-57 | -1.69E-46 |
| MS.gene059902 | MS.gene32769 | 0.850635 | 1.49E-60 | -1.69E-46 |
| MS.gene059960 | MS.gene32769 | 0.887956 | 9.04E-73 | -1.69E-46 |
| MS.gene059974 | MS.gene32769 | 0.800582 | 1.35E-48 | -1.69E-46 |
| MS.gene060193 | MS.gene32769 | 0.806405 | 8.36E-50 | -1.69E-46 |
| MS.gene060341 | MS.gene32769 | 0.811834 | 5.74E-51 | -1.69E-46 |
| MS.gene060356 | MS.gene32769 | 0.820482 | 6.70E-53 | -1.69E-46 |
| MS.gene060482 | MS.gene32769 | 0.835054 | 2.10E-56 | -1.69E-46 |
| MS.gene060703 | MS.gene32769 | 0.808489 | 3.02E-50 | -1.69E-46 |
| MS.gene060727 | MS.gene32769 | 0.810488 | 1.12E-50 | -1.69E-46 |
| MS.gene060728 | MS.gene32769 | 0.825816 | 3.81E-54 | -1.69E-46 |
| MS.gene060762 | MS.gene32769 | 0.827513 | 1.50E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene32769 | 0.801308 | 9.57E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene32769 | 0.885965 | 5.15E-72 | -1.69E-46 |
| MS.gene060818 | MS.gene32769 | 0.87651 | 1.32E-68 | -1.69E-46 |
| MS.gene060835 | MS.gene32769 | 0.838379 | 2.98E-57 | -1.69E-46 |
| MS.gene060837 | MS.gene32769 | 0.828577 | 8.32E-55 | -1.69E-46 |
| MS.gene06092 | MS.gene32769 | 0.843982 | 1.00E-58 | -1.69E-46 |
| MS.gene061041 | MS.gene32769 | 0.850608 | 1.52E-60 | -1.69E-46 |
| MS.gene061051 | MS.gene32769 | -0.809765 | 1.61E-50 | -1.69E-46 |
| MS.gene061134 | MS.gene32769 | 0.83693 | 7.01E-57 | -1.69E-46 |
| MS.gene061150 | MS.gene32769 | 0.854558 | 1.14E-61 | -1.69E-46 |
| MS.gene061200 | MS.gene32769 | 0.891745 | 3.00E-74 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32769.t1 | MTR_3g104950 | 95.833 | 120 | 5 | 0 | 1 | 120 | 1 | 120 | 4.31e-78 | 226 |
| MS.gene32769.t1 | MTR_1g015290 | 65.323 | 124 | 38 | 2 | 1 | 119 | 1 | 124 | 1.88e-47 | 149 |
| MS.gene32769.t1 | MTR_4g125600 | 57.025 | 121 | 45 | 5 | 1 | 119 | 1 | 116 | 1.86e-34 | 115 |
| MS.gene32769.t1 | MTR_2g041260 | 54.918 | 122 | 49 | 3 | 1 | 119 | 1 | 119 | 8.41e-24 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32769.t1 | AT2G06520 | 52.427 | 103 | 46 | 1 | 20 | 119 | 13 | 115 | 2.31e-24 | 90.1 |
Find 22 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATACTTGGAGCTGTTAT+TGG | 0.192002 | 3.1:+82888068 | MS.gene32769:CDS |
| TTTGACTGTGTGATCCTCTT+TGG | 0.259959 | 3.1:-82887870 | None:intergenic |
| CTATACTTGGAGCTGTTATT+GGG | 0.283981 | 3.1:+82888069 | MS.gene32769:CDS |
| ATTAATTAAGCTCTCTTAAC+CGG | 0.332183 | 3.1:-82888107 | None:intergenic |
| TTTGGTTGTGCGATGGGTAA+TGG | 0.371937 | 3.1:-82887852 | None:intergenic |
| AGATGCATTAATTTCAACCT+TGG | 0.419109 | 3.1:-82887895 | None:intergenic |
| TAATGCATCTTTGAAAGAAA+AGG | 0.433518 | 3.1:+82887908 | MS.gene32769:CDS |
| CCTGATGTGGCTCATGCAGC+TGG | 0.437171 | 3.1:+82887978 | MS.gene32769:CDS |
| ATCCTCTTTGGTTGTGCGAT+GGG | 0.483107 | 3.1:-82887858 | None:intergenic |
| TGGGGTTTCCAACTTTGATC+CGG | 0.483190 | 3.1:+82888088 | MS.gene32769:CDS |
| CCAGCTGCATGAGCCACATC+AGG | 0.505992 | 3.1:-82887978 | None:intergenic |
| GATCCTCTTTGGTTGTGCGA+TGG | 0.509446 | 3.1:-82887859 | None:intergenic |
| CTCCATGGTTATACCTGATG+TGG | 0.524546 | 3.1:+82887965 | MS.gene32769:CDS |
| TCTCTTAACCGGATCAAAGT+TGG | 0.532912 | 3.1:-82888096 | None:intergenic |
| TGCATCCTTGACAGCCTCCA+TGG | 0.539158 | 3.1:+82887950 | MS.gene32769:CDS |
| GCTGTGCTAACAGCTATACT+TGG | 0.540967 | 3.1:+82888056 | MS.gene32769:CDS |
| TATACTTGGAGCTGTTATTG+GGG | 0.562747 | 3.1:+82888070 | MS.gene32769:CDS |
| TATAACCATGGAGGCTGTCA+AGG | 0.564152 | 3.1:-82887955 | None:intergenic |
| TACCCATCGCACAACCAAAG+AGG | 0.604798 | 3.1:+82887856 | MS.gene32769:CDS |
| GATCACACAGTCAAAATCCA+AGG | 0.634917 | 3.1:+82887878 | MS.gene32769:CDS |
| AGCCACATCAGGTATAACCA+TGG | 0.670542 | 3.1:-82887967 | None:intergenic |
| CACATCAGGTATAACCATGG+AGG | 0.712255 | 3.1:-82887964 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGCATCTTTGAAAGAAA+AGG | + | chr3.1:82887908-82887927 | MS.gene32769:CDS | 25.0% |
| AAATGCTTAGCAAAAAGTTC+TGG | - | chr3.1:82888025-82888044 | None:intergenic | 30.0% | |
| AGATGCATTAATTTCAACCT+TGG | - | chr3.1:82887898-82887917 | None:intergenic | 30.0% | |
| TAATGGCCTGAAAAATGATT+CGG | - | chr3.1:82887838-82887857 | None:intergenic | 30.0% | |
| ! | TTTTTGCTAAGCATTTTTGC+TGG | + | chr3.1:82888029-82888048 | MS.gene32769:CDS | 30.0% |
| !! | ATGGATTTTTCAGCTTGTTT+TGG | - | chr3.1:82887812-82887831 | None:intergenic | 30.0% |
| !! | TGGATTTTTCAGCTTGTTTT+GGG | - | chr3.1:82887811-82887830 | None:intergenic | 30.0% |
| CTATACTTGGAGCTGTTATT+GGG | + | chr3.1:82888069-82888088 | MS.gene32769:CDS | 35.0% | |
| TATACTTGGAGCTGTTATTG+GGG | + | chr3.1:82888070-82888089 | MS.gene32769:CDS | 35.0% | |
| GATCACACAGTCAAAATCCA+AGG | + | chr3.1:82887878-82887897 | MS.gene32769:CDS | 40.0% | |
| GCTATACTTGGAGCTGTTAT+TGG | + | chr3.1:82888068-82888087 | MS.gene32769:CDS | 40.0% | |
| TCTCTTAACCGGATCAAAGT+TGG | - | chr3.1:82888099-82888118 | None:intergenic | 40.0% | |
| TTTGACTGTGTGATCCTCTT+TGG | - | chr3.1:82887873-82887892 | None:intergenic | 40.0% | |
| ! | CTGAAAAATGATTCGGAGGA+TGG | - | chr3.1:82887831-82887850 | None:intergenic | 40.0% |
| ! | TTGCTAAGCATTTTTGCTGG+TGG | + | chr3.1:82888032-82888051 | MS.gene32769:CDS | 40.0% |
| !! | AGCAAAAAGTTCTGGAGAGA+TGG | - | chr3.1:82888017-82888036 | None:intergenic | 40.0% |
| !! | CATCCTCCGAATCATTTTTC+AGG | + | chr3.1:82887829-82887848 | MS.gene32769:CDS | 40.0% |
| AGCCACATCAGGTATAACCA+TGG | - | chr3.1:82887970-82887989 | None:intergenic | 45.0% | |
| ATCCTCTTTGGTTGTGCGAT+GGG | - | chr3.1:82887861-82887880 | None:intergenic | 45.0% | |
| CACATCAGGTATAACCATGG+AGG | - | chr3.1:82887967-82887986 | None:intergenic | 45.0% | |
| CTCCATGGTTATACCTGATG+TGG | + | chr3.1:82887965-82887984 | MS.gene32769:CDS | 45.0% | |
| GCTGTGCTAACAGCTATACT+TGG | + | chr3.1:82888056-82888075 | MS.gene32769:CDS | 45.0% | |
| TATAACCATGGAGGCTGTCA+AGG | - | chr3.1:82887958-82887977 | None:intergenic | 45.0% | |
| TGGGGTTTCCAACTTTGATC+CGG | + | chr3.1:82888088-82888107 | MS.gene32769:CDS | 45.0% | |
| TTTGGTTGTGCGATGGGTAA+TGG | - | chr3.1:82887855-82887874 | None:intergenic | 45.0% | |
| ! | TGGCCTGAAAAATGATTCGG+AGG | - | chr3.1:82887835-82887854 | None:intergenic | 45.0% |
| !! | TTTTGGGTAGCAGAACTCAC+TGG | - | chr3.1:82887795-82887814 | None:intergenic | 45.0% |
| GATCCTCTTTGGTTGTGCGA+TGG | - | chr3.1:82887862-82887881 | None:intergenic | 50.0% | |
| TACCCATCGCACAACCAAAG+AGG | + | chr3.1:82887856-82887875 | MS.gene32769:CDS | 50.0% | |
| TGCATCCTTGACAGCCTCCA+TGG | + | chr3.1:82887950-82887969 | MS.gene32769:CDS | 55.0% | |
| CCAGCTGCATGAGCCACATC+AGG | - | chr3.1:82887981-82888000 | None:intergenic | 60.0% | |
| CCTGATGTGGCTCATGCAGC+TGG | + | chr3.1:82887978-82887997 | MS.gene32769:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 82887762 | 82888124 | 82887762 | ID=MS.gene32769 |
| chr3.1 | mRNA | 82887762 | 82888124 | 82887762 | ID=MS.gene32769.t1;Parent=MS.gene32769 |
| chr3.1 | exon | 82887762 | 82888124 | 82887762 | ID=MS.gene32769.t1.exon1;Parent=MS.gene32769.t1 |
| chr3.1 | CDS | 82887762 | 82888124 | 82887762 | ID=cds.MS.gene32769.t1;Parent=MS.gene32769.t1 |
| Gene Sequence |
| Protein sequence |
