AlfalfaGEDB | MS.gene33021

M. sativa cultivar XinJiangDaYe / MS.gene33021

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF01936.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33021.t1	XP_003588975.1	97.8	267	6	0	1	267	40	306	3.30E-150	541.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33021.t1	G7I9W5	97.8	267	6	0	1	267	40	306	2.4e-150	541.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene33021.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene33021	0.86904	4.17E-66	-1.69E-46
MS.gene049171	MS.gene33021	0.859652	3.57E-63	-1.69E-46
MS.gene049173	MS.gene33021	0.819419	1.17E-52	-1.69E-46
MS.gene049217	MS.gene33021	0.847121	1.41E-59	-1.69E-46
MS.gene049222	MS.gene33021	0.87294	2.16E-67	-1.69E-46
MS.gene049285	MS.gene33021	0.861041	1.35E-63	-1.69E-46
MS.gene049320	MS.gene33021	0.831152	1.96E-55	-1.69E-46
MS.gene049427	MS.gene33021	0.879057	1.70E-69	-1.69E-46
MS.gene049609	MS.gene33021	0.856738	2.63E-62	-1.69E-46
MS.gene049633	MS.gene33021	0.819056	1.42E-52	-1.69E-46
MS.gene049805	MS.gene33021	0.862422	5.13E-64	-1.69E-46
MS.gene050163	MS.gene33021	0.851103	1.10E-60	-1.69E-46
MS.gene050165	MS.gene33021	0.828522	8.58E-55	-1.69E-46
MS.gene050333	MS.gene33021	0.804322	2.28E-49	-1.69E-46
MS.gene050432	MS.gene33021	0.832999	6.86E-56	-1.69E-46
MS.gene050504	MS.gene33021	0.835855	1.32E-56	-1.69E-46
MS.gene050507	MS.gene33021	0.812865	3.42E-51	-1.69E-46
MS.gene050518	MS.gene33021	0.832879	7.35E-56	-1.69E-46
MS.gene050538	MS.gene33021	0.869243	3.58E-66	-1.69E-46
MS.gene050577	MS.gene33021	0.843793	1.13E-58	-1.69E-46
MS.gene050593	MS.gene33021	0.840793	7.02E-58	-1.69E-46
MS.gene050612	MS.gene33021	0.811947	5.43E-51	-1.69E-46
MS.gene050670	MS.gene33021	0.844114	9.24E-59	-1.69E-46
MS.gene050699	MS.gene33021	0.818574	1.83E-52	-1.69E-46
MS.gene050747	MS.gene33021	0.886246	4.04E-72	-1.69E-46
MS.gene050770	MS.gene33021	0.801329	9.48E-49	-1.69E-46
MS.gene050772	MS.gene33021	0.851399	9.10E-61	-1.69E-46
MS.gene050781	MS.gene33021	0.812598	3.91E-51	-1.69E-46
MS.gene050783	MS.gene33021	0.816275	6.01E-52	-1.69E-46
MS.gene05080	MS.gene33021	0.840281	9.56E-58	-1.69E-46
MS.gene050812	MS.gene33021	0.866903	2.03E-65	-1.69E-46
MS.gene05081	MS.gene33021	0.819748	9.87E-53	-1.69E-46
MS.gene050868	MS.gene33021	0.805501	1.30E-49	-1.69E-46
MS.gene050871	MS.gene33021	0.803419	3.52E-49	-1.69E-46
MS.gene050950	MS.gene33021	0.88032	6.05E-70	-1.69E-46
MS.gene05100	MS.gene33021	0.85413	1.51E-61	-1.69E-46
MS.gene05105	MS.gene33021	0.844954	5.49E-59	-1.69E-46
MS.gene051228	MS.gene33021	0.813824	2.11E-51	-1.69E-46
MS.gene051387	MS.gene33021	0.843026	1.80E-58	-1.69E-46
MS.gene051582	MS.gene33021	0.84757	1.06E-59	-1.69E-46
MS.gene051617	MS.gene33021	0.810445	1.15E-50	-1.69E-46
MS.gene051666	MS.gene33021	0.923972	1.31E-89	-1.69E-46
MS.gene051694	MS.gene33021	0.846239	2.46E-59	-1.69E-46
MS.gene051734	MS.gene33021	0.814351	1.61E-51	-1.69E-46
MS.gene051810	MS.gene33021	0.823333	1.47E-53	-1.69E-46
MS.gene051838	MS.gene33021	0.838647	2.54E-57	-1.69E-46
MS.gene051858	MS.gene33021	0.846225	2.48E-59	-1.69E-46
MS.gene052164	MS.gene33021	0.842077	3.22E-58	-1.69E-46
MS.gene052271	MS.gene33021	0.816426	5.56E-52	-1.69E-46
MS.gene052290	MS.gene33021	0.816115	6.53E-52	-1.69E-46
MS.gene052310	MS.gene33021	0.813857	2.07E-51	-1.69E-46
MS.gene052322	MS.gene33021	0.81814	2.29E-52	-1.69E-46
MS.gene052379	MS.gene33021	0.851614	7.91E-61	-1.69E-46
MS.gene052421	MS.gene33021	0.837629	4.64E-57	-1.69E-46
MS.gene052481	MS.gene33021	0.832738	7.97E-56	-1.69E-46
MS.gene052484	MS.gene33021	0.833486	5.19E-56	-1.69E-46
MS.gene052485	MS.gene33021	0.821871	3.21E-53	-1.69E-46
MS.gene052486	MS.gene33021	0.801925	7.15E-49	-1.69E-46
MS.gene052501	MS.gene33021	0.839245	1.78E-57	-1.69E-46
MS.gene052503	MS.gene33021	0.825593	4.31E-54	-1.69E-46
MS.gene052505	MS.gene33021	0.831386	1.72E-55	-1.69E-46
MS.gene052615	MS.gene33021	0.846633	1.92E-59	-1.69E-46
MS.gene052823	MS.gene33021	0.817241	3.65E-52	-1.69E-46
MS.gene052825	MS.gene33021	0.81796	2.51E-52	-1.69E-46
MS.gene052870	MS.gene33021	0.835513	1.61E-56	-1.69E-46
MS.gene052872	MS.gene33021	0.83509	2.05E-56	-1.69E-46
MS.gene052982	MS.gene33021	0.827633	1.40E-54	-1.69E-46
MS.gene053009	MS.gene33021	0.822587	2.19E-53	-1.69E-46
MS.gene053103	MS.gene33021	0.84102	6.12E-58	-1.69E-46
MS.gene053104	MS.gene33021	0.858964	5.74E-63	-1.69E-46
MS.gene053107	MS.gene33021	0.881609	2.08E-70	-1.69E-46
MS.gene053203	MS.gene33021	0.822278	2.58E-53	-1.69E-46
MS.gene053207	MS.gene33021	0.865093	7.58E-65	-1.69E-46
MS.gene053213	MS.gene33021	0.857003	2.19E-62	-1.69E-46
MS.gene053286	MS.gene33021	0.85185	6.78E-61	-1.69E-46
MS.gene053322	MS.gene33021	0.874377	7.08E-68	-1.69E-46
MS.gene053436	MS.gene33021	0.81572	8.00E-52	-1.69E-46
MS.gene053574	MS.gene33021	0.852762	3.73E-61	-1.69E-46
MS.gene053579	MS.gene33021	0.854838	9.43E-62	-1.69E-46
MS.gene053626	MS.gene33021	0.804133	2.50E-49	-1.69E-46
MS.gene053632	MS.gene33021	0.875098	4.03E-68	-1.69E-46
MS.gene053824	MS.gene33021	0.850005	2.25E-60	-1.69E-46
MS.gene053916	MS.gene33021	0.824758	6.79E-54	-1.69E-46
MS.gene054408	MS.gene33021	0.86901	4.26E-66	-1.69E-46
MS.gene054595	MS.gene33021	0.814018	1.91E-51	-1.69E-46
MS.gene054600	MS.gene33021	0.85611	4.02E-62	-1.69E-46
MS.gene054642	MS.gene33021	0.868036	8.80E-66	-1.69E-46
MS.gene05470	MS.gene33021	0.82275	2.00E-53	-1.69E-46
MS.gene054802	MS.gene33021	0.812229	4.71E-51	-1.69E-46
MS.gene054803	MS.gene33021	0.808183	3.51E-50	-1.69E-46
MS.gene054804	MS.gene33021	0.81252	4.07E-51	-1.69E-46
MS.gene054884	MS.gene33021	-0.804212	2.41E-49	-1.69E-46
MS.gene054927	MS.gene33021	0.865893	4.24E-65	-1.69E-46
MS.gene054954	MS.gene33021	0.809844	1.55E-50	-1.69E-46
MS.gene05505	MS.gene33021	0.8473	1.26E-59	-1.69E-46
MS.gene055206	MS.gene33021	0.856741	2.62E-62	-1.69E-46
MS.gene055212	MS.gene33021	0.80415	2.48E-49	-1.69E-46
MS.gene055224	MS.gene33021	0.829297	5.57E-55	-1.69E-46
MS.gene055289	MS.gene33021	0.848239	6.96E-60	-1.69E-46
MS.gene055362	MS.gene33021	0.828288	9.77E-55	-1.69E-46
MS.gene055370	MS.gene33021	0.819083	1.40E-52	-1.69E-46
MS.gene055397	MS.gene33021	0.82574	3.97E-54	-1.69E-46
MS.gene055412	MS.gene33021	0.836958	6.90E-57	-1.69E-46
MS.gene055499	MS.gene33021	0.827915	1.20E-54	-1.69E-46
MS.gene055502	MS.gene33021	0.860291	2.29E-63	-1.69E-46
MS.gene055577	MS.gene33021	0.861258	1.16E-63	-1.69E-46
MS.gene055647	MS.gene33021	0.803952	2.73E-49	-1.69E-46
MS.gene055722	MS.gene33021	0.833686	4.62E-56	-1.69E-46
MS.gene055748	MS.gene33021	0.828078	1.10E-54	-1.69E-46
MS.gene055750	MS.gene33021	0.811175	7.99E-51	-1.69E-46
MS.gene055751	MS.gene33021	0.80816	3.55E-50	-1.69E-46
MS.gene05592	MS.gene33021	0.83058	2.71E-55	-1.69E-46
MS.gene055941	MS.gene33021	0.851886	6.63E-61	-1.69E-46
MS.gene056083	MS.gene33021	0.835428	1.69E-56	-1.69E-46
MS.gene056374	MS.gene33021	0.834069	3.71E-56	-1.69E-46
MS.gene056407	MS.gene33021	0.820097	8.21E-53	-1.69E-46
MS.gene056409	MS.gene33021	0.816422	5.58E-52	-1.69E-46
MS.gene056449	MS.gene33021	0.808498	3.01E-50	-1.69E-46
MS.gene056510	MS.gene33021	0.807507	4.89E-50	-1.69E-46
MS.gene056511	MS.gene33021	0.80493	1.71E-49	-1.69E-46
MS.gene056610	MS.gene33021	0.820367	7.12E-53	-1.69E-46
MS.gene056657	MS.gene33021	0.842955	1.88E-58	-1.69E-46
MS.gene056692	MS.gene33021	0.82102	5.04E-53	-1.69E-46
MS.gene056796	MS.gene33021	0.819319	1.24E-52	-1.69E-46
MS.gene056897	MS.gene33021	0.864428	1.22E-64	-1.69E-46
MS.gene057011	MS.gene33021	0.867955	9.34E-66	-1.69E-46
MS.gene057048	MS.gene33021	0.836374	9.72E-57	-1.69E-46
MS.gene057076	MS.gene33021	0.805248	1.46E-49	-1.69E-46
MS.gene057124	MS.gene33021	0.817029	4.08E-52	-1.69E-46
MS.gene057129	MS.gene33021	0.879711	9.97E-70	-1.69E-46
MS.gene057146	MS.gene33021	0.857913	1.18E-62	-1.69E-46
MS.gene057196	MS.gene33021	0.825408	4.77E-54	-1.69E-46
MS.gene057331	MS.gene33021	0.813026	3.15E-51	-1.69E-46
MS.gene057332	MS.gene33021	0.813114	3.02E-51	-1.69E-46
MS.gene057416	MS.gene33021	0.867046	1.83E-65	-1.69E-46
MS.gene057658	MS.gene33021	0.86083	1.57E-63	-1.69E-46
MS.gene057831	MS.gene33021	0.832623	8.50E-56	-1.69E-46
MS.gene057856	MS.gene33021	0.803954	2.72E-49	-1.69E-46
MS.gene057926	MS.gene33021	0.802783	4.76E-49	-1.69E-46
MS.gene058006	MS.gene33021	0.800256	1.57E-48	-1.69E-46
MS.gene058019	MS.gene33021	0.845004	5.32E-59	-1.69E-46
MS.gene058130	MS.gene33021	0.844595	6.86E-59	-1.69E-46
MS.gene058233	MS.gene33021	0.833145	6.31E-56	-1.69E-46
MS.gene058293	MS.gene33021	0.853273	2.66E-61	-1.69E-46
MS.gene058323	MS.gene33021	0.845201	4.71E-59	-1.69E-46
MS.gene058338	MS.gene33021	0.814303	1.65E-51	-1.69E-46
MS.gene058376	MS.gene33021	0.80714	5.85E-50	-1.69E-46
MS.gene05837	MS.gene33021	0.835641	1.49E-56	-1.69E-46
MS.gene058419	MS.gene33021	0.872322	3.48E-67	-1.69E-46
MS.gene058444	MS.gene33021	0.808767	2.63E-50	-1.69E-46
MS.gene058544	MS.gene33021	0.876129	1.78E-68	-1.69E-46
MS.gene058660	MS.gene33021	0.835099	2.04E-56	-1.69E-46
MS.gene058723	MS.gene33021	0.832563	8.80E-56	-1.69E-46
MS.gene058773	MS.gene33021	0.828466	8.85E-55	-1.69E-46
MS.gene058838	MS.gene33021	0.844339	8.04E-59	-1.69E-46
MS.gene05883	MS.gene33021	0.82351	1.33E-53	-1.69E-46
MS.gene059022	MS.gene33021	0.806801	6.90E-50	-1.69E-46
MS.gene059023	MS.gene33021	0.870702	1.20E-66	-1.69E-46
MS.gene059029	MS.gene33021	0.817027	4.08E-52	-1.69E-46
MS.gene05914	MS.gene33021	0.831162	1.95E-55	-1.69E-46
MS.gene05934	MS.gene33021	0.882132	1.35E-70	-1.69E-46
MS.gene059536	MS.gene33021	0.863819	1.90E-64	-1.69E-46
MS.gene059677	MS.gene33021	0.84768	9.92E-60	-1.69E-46
MS.gene059688	MS.gene33021	0.847458	1.14E-59	-1.69E-46
MS.gene059692	MS.gene33021	0.836035	1.18E-56	-1.69E-46
MS.gene059695	MS.gene33021	0.841838	3.73E-58	-1.69E-46
MS.gene059718	MS.gene33021	0.862159	6.18E-64	-1.69E-46
MS.gene059756	MS.gene33021	0.833715	4.55E-56	-1.69E-46
MS.gene059801	MS.gene33021	0.812576	3.95E-51	-1.69E-46
MS.gene059902	MS.gene33021	0.847484	1.12E-59	-1.69E-46
MS.gene059960	MS.gene33021	0.865592	5.28E-65	-1.69E-46
MS.gene060193	MS.gene33021	0.809166	2.16E-50	-1.69E-46
MS.gene060403	MS.gene33021	0.814631	1.40E-51	-1.69E-46
MS.gene060762	MS.gene33021	0.83832	3.08E-57	-1.69E-46
MS.gene06077	MS.gene33021	0.822396	2.42E-53	-1.69E-46
MS.gene060804	MS.gene33021	0.853581	2.17E-61	-1.69E-46
MS.gene060818	MS.gene33021	0.808836	2.55E-50	-1.69E-46
MS.gene060835	MS.gene33021	0.814135	1.80E-51	-1.69E-46
MS.gene060837	MS.gene33021	0.820039	8.47E-53	-1.69E-46
MS.gene06092	MS.gene33021	0.902352	1.06E-78	-1.69E-46
MS.gene061041	MS.gene33021	0.826404	2.76E-54	-1.69E-46
MS.gene061150	MS.gene33021	0.878903	1.93E-69	-1.69E-46
MS.gene061200	MS.gene33021	0.861938	7.21E-64	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33021.t1	MTR_1g015930	95.666	323	12	1	1	323	40	360	0.0	613
MS.gene33021.t1	MTR_1g019050	51.362	257	95	7	4	255	50	281	5.93e-70	220
MS.gene33021.t1	MTR_3g084750	47.984	248	125	3	11	254	39	286	2.90e-67	215
MS.gene33021.t1	MTR_3g084750	47.984	248	125	3	11	254	91	338	5.86e-67	216

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33021.t1	AT5G52010	72.659	267	73	0	1	267	61	327	3.06e-151	430
MS.gene33021.t1	AT4G12240	49.180	244	121	3	15	255	39	282	9.26e-71	224

Find 94 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGGATGTTGATAGGGCTTT+AGG	0.212583	1.1:+6126946	MS.gene33021:CDS
AAGGCTAGAGAGGCCAATTT+AGG	0.226076	1.1:+6126910	MS.gene33021:CDS
GAATGGTGAGGTTACGGATA+TGG	0.264144	1.1:+6127008	MS.gene33021:CDS
GGGATGTTGATAGGGCTTTA+GGG	0.271261	1.1:+6126947	MS.gene33021:CDS
ACGACAACAATGGATGATTT+TGG	0.273875	1.1:+6127081	MS.gene33021:CDS
AACAACAACAGTCCCTAAAT+TGG	0.303839	1.1:-6126923	None:intergenic
GAAGAAGAGGCAAAAGTATA+AGG	0.310905	1.1:+6126648	MS.gene33021:CDS
TTGTTGTTGGGGATGTTGAT+AGG	0.320262	1.1:+6126938	MS.gene33021:CDS
ATTTAGGGACTGTTGTTGTT+GGG	0.328367	1.1:+6126926	MS.gene33021:CDS
AGAAGTATAATGATGCTGTT+AGG	0.333113	1.1:+6126692	MS.gene33021:CDS
TGGATTTGGTTCCAAGGAAT+AGG	0.348659	1.1:+6127028	MS.gene33021:CDS
CTTTCATTCATCTTCCTCAA+TGG	0.352874	1.1:+6126431	MS.gene33021:CDS
GAGAGATAGGGCGGCGTTGT+AGG	0.354988	1.1:-6126347	None:intergenic
CTTCTTCTATCCCTATTCCT+TGG	0.367662	1.1:-6127039	None:intergenic
TGAGGTTACGGATATGGATT+TGG	0.380915	1.1:+6127014	MS.gene33021:CDS
CCTCAACATCTCACTAAAAT+CGG	0.381248	1.1:-6126887	None:intergenic
AATTTAGGGACTGTTGTTGT+TGG	0.384290	1.1:+6126925	MS.gene33021:CDS
TTGTTCGTAGGTTCCTTGTT+TGG	0.390669	1.1:-6126266	None:intergenic
CTATGAGTAGAGGGATTGAT+TGG	0.397433	1.1:+6126842	MS.gene33021:CDS
GCCCAAAGTTGGGTATGGTT+TGG	0.398316	1.1:+6126726	MS.gene33021:CDS
GGATTTGGTTCCAAGGAATA+GGG	0.400811	1.1:+6127029	MS.gene33021:CDS
GCCAAACCATACCCAACTTT+GGG	0.407117	1.1:-6126727	None:intergenic
AGGGAGGCATGCTGATTTGT+GGG	0.407902	1.1:+6126966	MS.gene33021:CDS
TTTCATTCATCTTCCTCAAT+GGG	0.409031	1.1:+6126432	MS.gene33021:CDS
AAAACAAAGTCTGTGTCCTC+TGG	0.411202	1.1:+6126293	MS.gene33021:CDS
CGGCGAGGGATTTGAGAGAT+AGG	0.412332	1.1:-6126360	None:intergenic
AGGCTAGAGAGGCCAATTTA+GGG	0.414886	1.1:+6126911	MS.gene33021:CDS
AGCCAAACCATACCCAACTT+TGG	0.420939	1.1:-6126728	None:intergenic
GAACCGTTTGAATTCTTTGA+AGG	0.425604	1.1:+6126624	MS.gene33021:CDS
CTTCGGAGTTGAGGAGAGCC+GGG	0.432660	1.1:+6126749	MS.gene33021:CDS
AAATCCCTCGCCGAACGCTT+TGG	0.435682	1.1:+6126370	MS.gene33021:CDS
TAAGCCTCAAGCTGCTGATT+GGG	0.436038	1.1:+6126795	MS.gene33021:CDS
ATAAGCCTCAAGCTGCTGAT+TGG	0.442172	1.1:+6126794	MS.gene33021:CDS
GCTTCGGAGTTGAGGAGAGC+CGG	0.446052	1.1:+6126748	MS.gene33021:CDS
TAGGGAGGCATGCTGATTTG+TGG	0.446184	1.1:+6126965	MS.gene33021:CDS
TCCCCTTCAAAGAATTCAAA+CGG	0.451680	1.1:-6126627	None:intergenic
ACAGAACATACGTAAGGTTC+AGG	0.453549	1.1:-6126520	None:intergenic
GGTGACGTCGCCAAAGCGTT+CGG	0.459296	1.1:-6126380	None:intergenic
AGTTGGGTATGGTTTGGCTT+CGG	0.460925	1.1:+6126732	MS.gene33021:CDS
AGAGATAGGGCGGCGTTGTA+GGG	0.461281	1.1:-6126346	None:intergenic
TGATGATACCTTTGCGTTCG+AGG	0.484797	1.1:-6126492	None:intergenic
TTGAAGCCCAAAGTTGGGTA+TGG	0.495748	1.1:+6126721	MS.gene33021:CDS
TGTTGTTGGGGATGTTGATA+GGG	0.498608	1.1:+6126939	MS.gene33021:CDS
GAAGTATAATGATGCTGTTA+GGG	0.500181	1.1:+6126693	MS.gene33021:CDS
AGCGTGGCGGTTGGCGTAAG+CGG	0.500548	1.1:-6126410	None:intergenic
TCTGATGAAGAAGATGAAGA+TGG	0.507897	1.1:+6127189	MS.gene33021:CDS
CTGATGAAGAAGATGAAGAT+GGG	0.509060	1.1:+6127190	MS.gene33021:CDS
CTCACTAAAATCGGAATCAT+CGG	0.517860	1.1:-6126878	None:intergenic
CGCGTGGTGGTTTGTTGTCG+AGG	0.523493	1.1:-6126318	None:intergenic
CGGATATGGATTTGGTTCCA+AGG	0.531339	1.1:+6127022	MS.gene33021:CDS
CCGTGGAATGCTGTTGAGAA+TGG	0.533034	1.1:+6126991	MS.gene33021:CDS
GTCGCCAAAGCGTTCGGCGA+GGG	0.534340	1.1:-6126374	None:intergenic
TTTAGGGACTGTTGTTGTTG+GGG	0.539520	1.1:+6126927	MS.gene33021:CDS
AACCGTTTGAATTCTTTGAA+GGG	0.544942	1.1:+6126625	MS.gene33021:CDS
GGCGAGGGATTTGAGAGATA+GGG	0.558230	1.1:-6126359	None:intergenic
TGTTGAGAATGGTGAGGTTA+CGG	0.558545	1.1:+6127002	MS.gene33021:CDS
AAACAAAGTCTGTGTCCTCT+GGG	0.558785	1.1:+6126294	MS.gene33021:CDS
ATGAATGAAAGCGTGGCGGT+TGG	0.572273	1.1:-6126419	None:intergenic
ATTCTTTGAAGGGGAAGAAG+AGG	0.573313	1.1:+6126635	MS.gene33021:CDS
AAAGCCCAATCAGCAGCTTG+AGG	0.574075	1.1:-6126799	None:intergenic
CTTTGCGTTCGAGGAAGTCG+AGG	0.575865	1.1:-6126483	None:intergenic
GATGTTGAGGAAGGCTAGAG+AGG	0.579429	1.1:+6126900	MS.gene33021:CDS
CGTTGGTTGAGAACCCATTG+AGG	0.579899	1.1:-6126445	None:intergenic
TGATGCATTCTATGAGTAGA+GGG	0.580660	1.1:+6126833	MS.gene33021:CDS
ATGGTTTGGCTTCGGAGTTG+AGG	0.585237	1.1:+6126740	MS.gene33021:CDS
AGCTTGAGGCTTATCCTCCA+CGG	0.593303	1.1:-6126785	None:intergenic
ACCGTTTGAATTCTTTGAAG+GGG	0.594952	1.1:+6126626	MS.gene33021:CDS
ATGATGCATTCTATGAGTAG+AGG	0.597154	1.1:+6126832	MS.gene33021:CDS
CTCGACTTCCTCGAACGCAA+AGG	0.597673	1.1:+6126484	MS.gene33021:CDS
GATAGGGCGGCGTTGTAGGG+TGG	0.601999	1.1:-6126343	None:intergenic
GTTGGCGTAAGCGGAGATGT+CGG	0.613403	1.1:-6126401	None:intergenic
AACAACAACGACGACAACAA+TGG	0.614821	1.1:+6127071	MS.gene33021:CDS
CCATTCTCAACAGCATTCCA+CGG	0.619851	1.1:-6126991	None:intergenic
TTGTAGGGTGGTCCGCGTGG+TGG	0.626810	1.1:-6126331	None:intergenic
CCACAGACAGAACATACGTA+AGG	0.633305	1.1:-6126526	None:intergenic
TTCGGAGTTGAGGAGAGCCG+GGG	0.646906	1.1:+6126750	MS.gene33021:CDS
ATGTTGATAGGGCTTTAGGG+AGG	0.654086	1.1:+6126950	MS.gene33021:CDS
GAACATACGTAAGGTTCAGG+TGG	0.654767	1.1:-6126517	None:intergenic
GTTGGAGTATAGTGATGATG+AGG	0.656125	1.1:+6127152	MS.gene33021:CDS
CGTCGCCAAAGCGTTCGGCG+AGG	0.664095	1.1:-6126375	None:intergenic
GAGGAAGATGAATGAAAGCG+TGG	0.667208	1.1:-6126426	None:intergenic
CCTTACGTATGTTCTGTCTG+TGG	0.667545	1.1:+6126526	MS.gene33021:CDS
ATGCTGATTTGTGGGTGCCG+TGG	0.676696	1.1:+6126974	MS.gene33021:CDS
CGGGGTGTTTGTGAAGACCG+TGG	0.682036	1.1:+6126768	MS.gene33021:CDS
TATAAGGAGAGATTTGTGAG+TGG	0.683928	1.1:+6126664	MS.gene33021:CDS
TGATGATGAGGATTCTGATG+AGG	0.686403	1.1:+6127164	MS.gene33021:CDS
GCGTTGTAGGGTGGTCCGCG+TGG	0.689378	1.1:-6126334	None:intergenic
GTTTGTTGTCGAGGTCCCAG+AGG	0.689800	1.1:-6126309	None:intergenic
CACGGTCTTCACAAACACCC+CGG	0.699093	1.1:-6126767	None:intergenic
CTCGACAACAAACCACCACG+CGG	0.706944	1.1:+6126319	MS.gene33021:CDS
GAGGGATTTGAGAGATAGGG+CGG	0.708759	1.1:-6126356	None:intergenic
GAATGCTGTTGAGAATGGTG+AGG	0.763448	1.1:+6126996	MS.gene33021:CDS
GGTGTTTGTGAAGACCGTGG+AGG	0.763606	1.1:+6126771	MS.gene33021:CDS
GAAGATGAATGAAAGCGTGG+CGG	0.793373	1.1:-6126423	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATGGAAGAAGATTTTATGT+TGG	+	chr1.1:6127134-6127153	MS.gene33021:CDS	25.0%
!!!	TTTGTTTTGTTTTTGTTCGT+AGG	-	chr1.1:6126281-6126300	None:intergenic	25.0%
	AACCGTTTGAATTCTTTGAA+GGG	+	chr1.1:6126625-6126644	MS.gene33021:CDS	30.0%
	GAAGTATAATGATGCTGTTA+GGG	+	chr1.1:6126693-6126712	MS.gene33021:CDS	30.0%
	TTTCATTCATCTTCCTCAAT+GGG	+	chr1.1:6126432-6126451	MS.gene33021:CDS	30.0%
!	AGAAGTATAATGATGCTGTT+AGG	+	chr1.1:6126692-6126711	MS.gene33021:CDS	30.0%
!!!	GATGTGTTGTTTTTTCATGA+GGG	+	chr1.1:6127105-6127124	MS.gene33021:CDS	30.0%
!!!	TGATGTGTTGTTTTTTCATG+AGG	+	chr1.1:6127104-6127123	MS.gene33021:CDS	30.0%
	AACAACAACAGTCCCTAAAT+TGG	-	chr1.1:6126926-6126945	None:intergenic	35.0%
	AATTTAGGGACTGTTGTTGT+TGG	+	chr1.1:6126925-6126944	MS.gene33021:CDS	35.0%
	ATGATGCATTCTATGAGTAG+AGG	+	chr1.1:6126832-6126851	MS.gene33021:CDS	35.0%
	CCTCAACATCTCACTAAAAT+CGG	-	chr1.1:6126890-6126909	None:intergenic	35.0%
	CTCACTAAAATCGGAATCAT+CGG	-	chr1.1:6126881-6126900	None:intergenic	35.0%
	CTGATGAAGAAGATGAAGAT+GGG	+	chr1.1:6127190-6127209	MS.gene33021:CDS	35.0%
	CTTTCATTCATCTTCCTCAA+TGG	+	chr1.1:6126431-6126450	MS.gene33021:CDS	35.0%
	GAACCGTTTGAATTCTTTGA+AGG	+	chr1.1:6126624-6126643	MS.gene33021:CDS	35.0%
	TATAAGGAGAGATTTGTGAG+TGG	+	chr1.1:6126664-6126683	MS.gene33021:CDS	35.0%
	TCCCCTTCAAAGAATTCAAA+CGG	-	chr1.1:6126630-6126649	None:intergenic	35.0%
	TCTGATGAAGAAGATGAAGA+TGG	+	chr1.1:6127189-6127208	MS.gene33021:CDS	35.0%
	TGATGCATTCTATGAGTAGA+GGG	+	chr1.1:6126833-6126852	MS.gene33021:CDS	35.0%
!	ACCGTTTGAATTCTTTGAAG+GGG	+	chr1.1:6126626-6126645	MS.gene33021:CDS	35.0%
!	ACGACAACAATGGATGATTT+TGG	+	chr1.1:6127081-6127100	MS.gene33021:CDS	35.0%
!	ATTTAGGGACTGTTGTTGTT+GGG	+	chr1.1:6126926-6126945	MS.gene33021:CDS	35.0%
!	GAAGAAGAGGCAAAAGTATA+AGG	+	chr1.1:6126648-6126667	MS.gene33021:CDS	35.0%
!	TTTTAGTGAGATGTTGAGGA+AGG	+	chr1.1:6126891-6126910	MS.gene33021:CDS	35.0%
!!	AGGGATTGATTGGTTGTTTT+TGG	+	chr1.1:6126852-6126871	MS.gene33021:CDS	35.0%
!!!	AGATTTTGAAGCCCAAAGTT+GGG	+	chr1.1:6126716-6126735	MS.gene33021:CDS	35.0%
	AAAACAAAGTCTGTGTCCTC+TGG	+	chr1.1:6126293-6126312	MS.gene33021:CDS	40.0%
	AAACAAAGTCTGTGTCCTCT+GGG	+	chr1.1:6126294-6126313	MS.gene33021:CDS	40.0%
	AACAACAACGACGACAACAA+TGG	+	chr1.1:6127071-6127090	MS.gene33021:CDS	40.0%
	ACAGAACATACGTAAGGTTC+AGG	-	chr1.1:6126523-6126542	None:intergenic	40.0%
	CTATGAGTAGAGGGATTGAT+TGG	+	chr1.1:6126842-6126861	MS.gene33021:CDS	40.0%
	CTTCTTCTATCCCTATTCCT+TGG	-	chr1.1:6127042-6127061	None:intergenic	40.0%
	TGAGGTTACGGATATGGATT+TGG	+	chr1.1:6127014-6127033	MS.gene33021:CDS	40.0%
	TGTTGAGAATGGTGAGGTTA+CGG	+	chr1.1:6127002-6127021	MS.gene33021:CDS	40.0%
	TGTTGTTGGGGATGTTGATA+GGG	+	chr1.1:6126939-6126958	MS.gene33021:CDS	40.0%
	TTGTTCGTAGGTTCCTTGTT+TGG	-	chr1.1:6126269-6126288	None:intergenic	40.0%
!	AGTCGAGGTTTTTACGTTCA+CGG	-	chr1.1:6126471-6126490	None:intergenic	40.0%
!	ATTCTTTGAAGGGGAAGAAG+AGG	+	chr1.1:6126635-6126654	MS.gene33021:CDS	40.0%
!	GGATTTGGTTCCAAGGAATA+GGG	+	chr1.1:6127029-6127048	MS.gene33021:CDS	40.0%
!	GTTGGAGTATAGTGATGATG+AGG	+	chr1.1:6127152-6127171	MS.gene33021:CDS	40.0%
!	TGATGATGAGGATTCTGATG+AGG	+	chr1.1:6127164-6127183	MS.gene33021:CDS	40.0%
!	TGGATTTGGTTCCAAGGAAT+AGG	+	chr1.1:6127028-6127047	MS.gene33021:CDS	40.0%
!	TTGTTGTTGGGGATGTTGAT+AGG	+	chr1.1:6126938-6126957	MS.gene33021:CDS	40.0%
!	TTTAGGGACTGTTGTTGTTG+GGG	+	chr1.1:6126927-6126946	MS.gene33021:CDS	40.0%
!!	CCGATTTTAGTGAGATGTTG+AGG	+	chr1.1:6126887-6126906	MS.gene33021:CDS	40.0%
!!!	GAGATTTTGAAGCCCAAAGT+TGG	+	chr1.1:6126715-6126734	MS.gene33021:CDS	40.0%
	AAGGCTAGAGAGGCCAATTT+AGG	+	chr1.1:6126910-6126929	MS.gene33021:CDS	45.0%
	AGCCAAACCATACCCAACTT+TGG	-	chr1.1:6126731-6126750	None:intergenic	45.0%
	AGGCTAGAGAGGCCAATTTA+GGG	+	chr1.1:6126911-6126930	MS.gene33021:CDS	45.0%
	ATAAGCCTCAAGCTGCTGAT+TGG	+	chr1.1:6126794-6126813	MS.gene33021:CDS	45.0%
	CCACAGACAGAACATACGTA+AGG	-	chr1.1:6126529-6126548	None:intergenic	45.0%
	CCATTCTCAACAGCATTCCA+CGG	-	chr1.1:6126994-6127013	None:intergenic	45.0%
	CCTTACGTATGTTCTGTCTG+TGG	+	chr1.1:6126526-6126545	MS.gene33021:CDS	45.0%
	GAACATACGTAAGGTTCAGG+TGG	-	chr1.1:6126520-6126539	None:intergenic	45.0%
	GAATGCTGTTGAGAATGGTG+AGG	+	chr1.1:6126996-6127015	MS.gene33021:CDS	45.0%
	GAATGGTGAGGTTACGGATA+TGG	+	chr1.1:6127008-6127027	MS.gene33021:CDS	45.0%
	GAGGAAGATGAATGAAAGCG+TGG	-	chr1.1:6126429-6126448	None:intergenic	45.0%
	GCCAAACCATACCCAACTTT+GGG	-	chr1.1:6126730-6126749	None:intergenic	45.0%
	TGATGATACCTTTGCGTTCG+AGG	-	chr1.1:6126495-6126514	None:intergenic	45.0%
	TTGAAGCCCAAAGTTGGGTA+TGG	+	chr1.1:6126721-6126740	MS.gene33021:CDS	45.0%
!	AGTTGGGTATGGTTTGGCTT+CGG	+	chr1.1:6126732-6126751	MS.gene33021:CDS	45.0%
!	CGGATATGGATTTGGTTCCA+AGG	+	chr1.1:6127022-6127041	MS.gene33021:CDS	45.0%
!	GAAGATGAATGAAAGCGTGG+CGG	-	chr1.1:6126426-6126445	None:intergenic	45.0%
!	GGGATGTTGATAGGGCTTTA+GGG	+	chr1.1:6126947-6126966	MS.gene33021:CDS	45.0%
!	TAAGCCTCAAGCTGCTGATT+GGG	+	chr1.1:6126795-6126814	MS.gene33021:CDS	45.0%
!	TTTTCATGAGGGCGAGGAAA+TGG	+	chr1.1:6127116-6127135	MS.gene33021:CDS	45.0%
!!	ATGTTGATAGGGCTTTAGGG+AGG	+	chr1.1:6126950-6126969	MS.gene33021:CDS	45.0%
!!!	GTTGTTTTTTCATGAGGGCG+AGG	+	chr1.1:6127110-6127129	MS.gene33021:CDS	45.0%
	AAAGCCCAATCAGCAGCTTG+AGG	-	chr1.1:6126802-6126821	None:intergenic	50.0%
	AGCTTGAGGCTTATCCTCCA+CGG	-	chr1.1:6126788-6126807	None:intergenic	50.0%
	CCGTGGAATGCTGTTGAGAA+TGG	+	chr1.1:6126991-6127010	MS.gene33021:CDS	50.0%
	GAGGGATTTGAGAGATAGGG+CGG	-	chr1.1:6126359-6126378	None:intergenic	50.0%
	GATGTTGAGGAAGGCTAGAG+AGG	+	chr1.1:6126900-6126919	MS.gene33021:CDS	50.0%
	GCCCAAAGTTGGGTATGGTT+TGG	+	chr1.1:6126726-6126745	MS.gene33021:CDS	50.0%
	GGCGAGGGATTTGAGAGATA+GGG	-	chr1.1:6126362-6126381	None:intergenic	50.0%
!	ATGAATGAAAGCGTGGCGGT+TGG	-	chr1.1:6126422-6126441	None:intergenic	50.0%
!	ATGGTTTGGCTTCGGAGTTG+AGG	+	chr1.1:6126740-6126759	MS.gene33021:CDS	50.0%
!	CGTTGGTTGAGAACCCATTG+AGG	-	chr1.1:6126448-6126467	None:intergenic	50.0%
!	GGGGATGTTGATAGGGCTTT+AGG	+	chr1.1:6126946-6126965	MS.gene33021:CDS	50.0%
!	GGTTTTTACGTTCACGGCGT+TGG	-	chr1.1:6126465-6126484	None:intergenic	50.0%
!!	AGGGAGGCATGCTGATTTGT+GGG	+	chr1.1:6126966-6126985	MS.gene33021:CDS	50.0%
!!	TAGGGAGGCATGCTGATTTG+TGG	+	chr1.1:6126965-6126984	MS.gene33021:CDS	50.0%
	AAATCCCTCGCCGAACGCTT+TGG	+	chr1.1:6126370-6126389	MS.gene33021:CDS	55.0%
	AGAGATAGGGCGGCGTTGTA+GGG	-	chr1.1:6126349-6126368	None:intergenic	55.0%
	CACGGTCTTCACAAACACCC+CGG	-	chr1.1:6126770-6126789	None:intergenic	55.0%
	CGGCGAGGGATTTGAGAGAT+AGG	-	chr1.1:6126363-6126382	None:intergenic	55.0%
	CTCGACAACAAACCACCACG+CGG	+	chr1.1:6126319-6126338	MS.gene33021:CDS	55.0%
	CTCGACTTCCTCGAACGCAA+AGG	+	chr1.1:6126484-6126503	MS.gene33021:CDS	55.0%
	CTTTGCGTTCGAGGAAGTCG+AGG	-	chr1.1:6126486-6126505	None:intergenic	55.0%
	GGTGTTTGTGAAGACCGTGG+AGG	+	chr1.1:6126771-6126790	MS.gene33021:CDS	55.0%
	GTTGGCGTAAGCGGAGATGT+CGG	-	chr1.1:6126404-6126423	None:intergenic	55.0%
!!	ATGCTGATTTGTGGGTGCCG+TGG	+	chr1.1:6126974-6126993	MS.gene33021:CDS	55.0%
!!	GTTTGTTGTCGAGGTCCCAG+AGG	-	chr1.1:6126312-6126331	None:intergenic	55.0%
	CTTCGGAGTTGAGGAGAGCC+GGG	+	chr1.1:6126749-6126768	MS.gene33021:CDS	60.0%
	GAGAGATAGGGCGGCGTTGT+AGG	-	chr1.1:6126350-6126369	None:intergenic	60.0%
	GCTTCGGAGTTGAGGAGAGC+CGG	+	chr1.1:6126748-6126767	MS.gene33021:CDS	60.0%
	TTCGGAGTTGAGGAGAGCCG+GGG	+	chr1.1:6126750-6126769	MS.gene33021:CDS	60.0%
!	CGGGGTGTTTGTGAAGACCG+TGG	+	chr1.1:6126768-6126787	MS.gene33021:CDS	60.0%
!	GGTGACGTCGCCAAAGCGTT+CGG	-	chr1.1:6126383-6126402	None:intergenic	60.0%
!!	CGCGTGGTGGTTTGTTGTCG+AGG	-	chr1.1:6126321-6126340	None:intergenic	60.0%
	AGCGTGGCGGTTGGCGTAAG+CGG	-	chr1.1:6126413-6126432	None:intergenic	65.0%
	GATAGGGCGGCGTTGTAGGG+TGG	-	chr1.1:6126346-6126365	None:intergenic	65.0%
	TTGTAGGGTGGTCCGCGTGG+TGG	-	chr1.1:6126334-6126353	None:intergenic	65.0%
!	GTCGCCAAAGCGTTCGGCGA+GGG	-	chr1.1:6126377-6126396	None:intergenic	65.0%
	GCGTTGTAGGGTGGTCCGCG+TGG	-	chr1.1:6126337-6126356	None:intergenic	70.0%
!	CGTCGCCAAAGCGTTCGGCG+AGG	-	chr1.1:6126378-6126397	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	6126256	6127227	6126256	ID=MS.gene33021
chr1.1	mRNA	6126256	6127227	6126256	ID=MS.gene33021.t1;Parent=MS.gene33021
chr1.1	exon	6126256	6127227	6126256	ID=MS.gene33021.t1.exon1;Parent=MS.gene33021.t1
chr1.1	CDS	6126256	6127227	6126256	ID=cds.MS.gene33021.t1;Parent=MS.gene33021.t1

Gene Sequence

>MS.gene33021

ATGGTAAAAACCAAACAAGGAACCTACGAACAAAAACAAAACAAAGTCTGTGTCCTCTGGGACCTCGACAACAAACCACCACGCGGACCACCCTACAACGCCGCCCTATCTCTCAAATCCCTCGCCGAACGCTTTGGCGACGTCACCGACATCTCCGCTTACGCCAACCGCCACGCTTTCATTCATCTTCCTCAATGGGTTCTCAACCAACGCCGTGAACGTAAAAACCTCGACTTCCTCGAACGCAAAGGTATCATCAATCCACCTGAACCTTACGTATGTTCTGTCTGTGGTAGAAAATGTAAAACTAACGTAGATCTTAAGAAACACTTCAAACAGCTTCATCAACGTGAGAGACAGAAGAAGCTGAACCGTTTGAATTCTTTGAAGGGGAAGAAGAGGCAAAAGTATAAGGAGAGATTTGTGAGTGGTGATGAGAAGTATAATGATGCTGTTAGGGAGATTTTGAAGCCCAAAGTTGGGTATGGTTTGGCTTCGGAGTTGAGGAGAGCCGGGGTGTTTGTGAAGACCGTGGAGGATAAGCCTCAAGCTGCTGATTGGGCTTTGAAGAAGCAGATGATGCATTCTATGAGTAGAGGGATTGATTGGTTGTTTTTGGTTTCCGATGATTCCGATTTTAGTGAGATGTTGAGGAAGGCTAGAGAGGCCAATTTAGGGACTGTTGTTGTTGGGGATGTTGATAGGGCTTTAGGGAGGCATGCTGATTTGTGGGTGCCGTGGAATGCTGTTGAGAATGGTGAGGTTACGGATATGGATTTGGTTCCAAGGAATAGGGATAGAAGAAGAACATCAAGAACAACAACGACGACAACAATGGATGATTTTGGTGATGTGTTGTTTTTTCATGAGGGCGAGGAAATGGAAGAAGATTTTATGTTGGAGTATAGTGATGATGAGGATTCTGATGAGGATTCTGATGAAGAAGATGAAGATGGGTTTTATATTTATTGA

Protein sequence

>MS.gene33021.t1

MVKTKQGTYEQKQNKVCVLWDLDNKPPRGPPYNAALSLKSLAERFGDVTDISAYANRHAFIHLPQWVLNQRRERKNLDFLERKGIINPPEPYVCSVCGRKCKTNVDLKKHFKQLHQRERQKKLNRLNSLKGKKRQKYKERFVSGDEKYNDAVREILKPKVGYGLASELRRAGVFVKTVEDKPQAADWALKKQMMHSMSRGIDWLFLVSDDSDFSEMLRKAREANLGTVVVGDVDRALGRHADLWVPWNAVENGEVTDMDLVPRNRDRRRTSRTTTTTTMDDFGDVLFFHEGEEMEEDFMLEYSDDEDSDEDSDEEDEDGFYIY