AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34701

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34701.t1 MTR_8g062300 53.498 486 165 14 1 435 1 476 4.18e-154 446
MS.gene34701.t1 MTR_8g070950 50.746 469 161 11 1 435 1 433 6.76e-134 392
MS.gene34701.t1 MTR_8g062010 53.653 438 163 12 23 435 7 429 1.73e-131 386
MS.gene34701.t1 MTR_8g062310 50.340 441 164 10 17 435 20 427 1.01e-129 382
MS.gene34701.t1 MTR_8g062080 51.902 447 158 11 23 435 21 444 3.25e-128 378
MS.gene34701.t1 MTR_8g469020 45.203 469 178 10 23 435 5 450 1.26e-116 349
MS.gene34701.t1 MTR_8g058965 46.105 475 187 16 9 435 2 455 1.27e-112 339
MS.gene34701.t1 MTR_4g050250 44.394 437 171 14 1 382 1 420 2.23e-97 298
MS.gene34701.t1 MTR_8g467790 47.368 380 138 13 24 377 147 490 6.92e-92 287
MS.gene34701.t1 MTR_8g468810 37.029 478 242 14 1 435 1 462 3.40e-74 240
MS.gene34701.t1 MTR_8g058970 35.341 498 237 18 1 435 1 476 9.74e-68 223
MS.gene34701.t1 MTR_2g083840 35.794 447 240 14 25 435 21 456 5.87e-64 213
MS.gene34701.t1 MTR_4g032730 33.947 489 239 18 9 436 2 467 1.37e-60 204
MS.gene34701.t1 MTR_8g062270 33.121 471 226 21 23 435 14 453 5.88e-55 189
MS.gene34701.t1 MTR_8g468840 35.526 456 231 19 25 436 14 450 9.87e-55 188
MS.gene34701.t1 MTR_2g086020 75.652 115 28 0 321 435 200 314 4.81e-54 183
MS.gene34701.t1 MTR_2g086020 59.868 152 58 2 51 201 1 150 2.28e-49 171
MS.gene34701.t1 MTR_5g092960 31.933 476 259 16 7 436 6 462 1.74e-53 186
MS.gene34701.t1 MTR_8g014260 33.333 477 249 20 1 435 1 450 1.48e-52 183
MS.gene34701.t1 MTR_8g468810 36.693 387 188 13 1 346 1 371 5.62e-52 179
MS.gene34701.t1 MTR_8g468810 39.172 314 150 10 1 288 1 299 2.53e-50 173
MS.gene34701.t1 MTR_8g468810 39.172 314 150 10 1 288 1 299 3.08e-50 173
MS.gene34701.t1 MTR_8g468810 39.172 314 150 10 1 288 1 299 8.28e-50 173
MS.gene34701.t1 MTR_8g468760 32.671 453 228 17 21 435 5 418 4.72e-49 172
MS.gene34701.t1 MTR_8g467800 33.053 475 255 17 1 435 1 452 2.23e-47 169
MS.gene34701.t1 MTR_8g445780 34.217 415 222 17 1 389 1 390 1.44e-45 162
MS.gene34701.t1 MTR_8g014160 32.517 449 218 20 16 435 32 424 1.52e-40 150
MS.gene34701.t1 MTR_5g092960 31.759 381 204 12 7 346 6 371 3.68e-34 132
MS.gene34701.t1 MTR_4g024710 34.146 287 151 13 176 435 71 346 1.19e-29 118
MS.gene34701.t1 MTR_8g077460 28.436 422 234 16 25 435 10 374 5.05e-29 117
MS.gene34701.t1 MTR_8g467800 34.472 322 172 13 1 303 1 302 4.04e-28 114
MS.gene34701.t1 MTR_8g014210 39.785 186 97 7 260 435 50 230 9.68e-26 105
MS.gene34701.t1 MTR_3g099800 64.935 77 26 1 348 423 19 95 3.31e-25 99.8
MS.gene34701.t1 MTR_1g041245 28.638 426 242 16 23 435 9 385 4.38e-24 103
MS.gene34701.t1 MTR_7g115270 29.032 434 237 21 25 433 12 399 9.62e-22 97.1
MS.gene34701.t1 MTR_4g024745 33.453 278 150 12 164 415 12 280 2.69e-20 90.9
MS.gene34701.t1 MTR_7g098480 26.603 421 251 16 24 435 17 388 1.92e-17 84.7
MS.gene34701.t1 MTR_7g056447 35.204 196 109 7 20 210 4 186 3.32e-17 82.0
MS.gene34701.t1 MTR_5g021550 38.854 157 65 9 25 163 19 162 4.79e-17 78.6
MS.gene34701.t1 MTR_7g056433 26.603 421 255 16 25 435 12 388 8.81e-17 82.0
MS.gene34701.t1 MTR_5g080710 27.778 414 250 17 24 408 32 425 2.47e-16 81.3
MS.gene34701.t1 MTR_1089s0010 25.175 429 249 20 24 436 7 379 3.04e-16 80.5
MS.gene34701.t1 MTR_3g107160 26.854 391 226 16 25 401 10 354 3.49e-16 80.5
MS.gene34701.t1 MTR_7g056430 26.214 412 223 16 25 423 9 352 9.89e-16 80.5
MS.gene34701.t1 MTR_7g056420 26.521 411 223 17 25 423 9 352 1.05e-15 80.1
MS.gene34701.t1 MTR_7g007080 27.059 425 250 17 25 437 4 380 1.32e-15 78.6
MS.gene34701.t1 MTR_4g098590 28.993 407 206 22 27 403 1 354 3.55e-15 77.0
MS.gene34701.t1 MTR_3g082890 26.350 463 254 21 24 436 16 441 3.65e-15 77.4
MS.gene34701.t1 MTR_7g026500 23.185 427 270 12 23 435 12 394 4.75e-15 77.0
MS.gene34701.t1 MTR_7g034115 28.608 395 214 18 24 403 10 351 8.12e-15 76.3
MS.gene34701.t1 MTR_3g107120 28.607 402 214 16 25 401 9 362 1.22e-14 75.9
MS.gene34701.t1 MTR_7g034110 29.767 215 127 6 24 227 10 211 5.10e-14 73.6
MS.gene34701.t1 MTR_3g467400 23.256 430 247 16 24 435 2 366 5.26e-14 73.6
MS.gene34701.t1 MTR_1g085120 24.828 435 263 18 16 435 85 470 7.11e-14 73.6
MS.gene34701.t1 MTR_1g085120 24.828 435 263 18 16 435 160 545 8.14e-14 73.9
MS.gene34701.t1 MTR_7g055943 24.826 431 262 18 21 435 36 420 8.69e-14 73.2
MS.gene34701.t1 MTR_8g014230 44.048 84 44 3 341 424 13 93 1.02e-13 67.4
MS.gene34701.t1 MTR_2g007450 26.327 452 250 20 24 437 15 421 1.68e-13 72.4
MS.gene34701.t1 MTR_4g080787 25.527 474 274 21 25 439 19 472 3.04e-13 72.0
MS.gene34701.t1 MTR_3g108560 24.821 419 257 15 25 435 20 388 5.45e-13 70.5
MS.gene34701.t1 MTR_5g084070 26.437 435 263 20 24 428 12 419 8.44e-13 70.1
MS.gene34701.t1 MTR_6g011460 24.831 443 267 16 7 435 30 420 1.47e-12 69.3
MS.gene34701.t1 MTR_6g044560 26.360 239 144 7 2 213 155 388 2.87e-12 68.9
MS.gene34701.t1 MTR_7g032380 34.343 198 110 10 23 213 9 193 2.97e-12 68.2
MS.gene34701.t1 MTR_2g036470 24.057 424 253 19 32 435 21 395 4.37e-12 67.8
MS.gene34701.t1 MTR_3g071850 24.344 419 253 17 24 423 23 396 4.91e-12 68.6
MS.gene34701.t1 MTR_6g022360 26.276 392 225 17 21 403 39 375 5.73e-12 67.4
MS.gene34701.t1 MTR_3g107100 32.143 196 116 9 25 214 11 195 5.84e-12 67.4
MS.gene34701.t1 MTR_2g007500 28.533 375 197 20 23 380 12 332 1.18e-11 66.2
MS.gene34701.t1 MTR_4g024720 38.655 119 58 5 25 135 17 128 1.60e-11 63.9
MS.gene34701.t1 MTR_7g098510 31.443 194 116 9 24 212 17 198 2.84e-11 65.5
MS.gene34701.t1 MTR_4g056040 24.945 457 243 21 25 404 9 442 3.35e-11 65.5
MS.gene34701.t1 MTR_6g044572 27.982 218 123 10 24 213 1 212 4.04e-11 64.7
MS.gene34701.t1 MTR_5g008950 31.746 189 114 8 27 213 1 176 8.05e-11 63.9
MS.gene34701.t1 MTR_3g082890 33.333 198 107 10 25 213 97 278 9.44e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34701.t1 AT4G26340 25.688 436 282 14 24 435 1 418 2.28e-19 90.1
MS.gene34701.t1 AT4G13965 25.561 446 272 18 18 433 5 420 1.07e-17 85.1
MS.gene34701.t1 AT4G10400 25.058 431 281 15 24 435 1 408 1.63e-17 84.7
MS.gene34701.t1 AT4G10400 25.058 431 281 15 24 435 1 408 1.63e-17 84.7
MS.gene34701.t1 AT4G10400 25.058 431 281 15 24 435 1 408 1.63e-17 84.7
MS.gene34701.t1 AT3G42770 30.348 201 118 7 27 214 1 192 1.44e-13 73.2
MS.gene34701.t1 AT1G78750 24.783 460 275 20 24 433 17 455 2.07e-13 72.4
MS.gene34701.t1 AT1G78750 24.783 460 275 20 24 433 17 455 2.07e-13 72.4
MS.gene34701.t1 AT1G78760 24.036 441 272 16 14 412 1 420 3.76e-13 71.6
MS.gene34701.t1 AT2G04230 25.380 461 268 20 25 436 13 446 7.67e-13 70.5
MS.gene34701.t1 AT2G26860 31.220 205 111 10 24 214 1 189 1.24e-12 69.7
MS.gene34701.t1 AT2G26860 31.220 205 111 10 24 214 1 189 1.24e-12 69.7
MS.gene34701.t1 AT1G55030 25.702 463 281 20 4 435 1 431 3.68e-12 68.2
MS.gene34701.t1 AT5G53635 25.000 448 273 15 27 435 2 425 2.81e-11 65.5
MS.gene34701.t1 AT5G60610 24.570 407 253 21 24 400 1 383 3.68e-11 65.1
MS.gene34701.t1 AT5G60610 24.570 407 253 21 24 400 1 383 3.68e-11 65.1
MS.gene34701.t1 AT4G09920 27.103 214 138 6 24 231 1 202 4.48e-11 63.9
MS.gene34701.t1 AT4G09920 27.103 214 138 6 24 231 1 202 4.48e-11 63.9
MS.gene34701.t1 AT4G09920 27.103 214 138 6 24 231 1 202 4.48e-11 63.9
MS.gene34701.t1 AT1G55660 32.161 199 111 11 24 211 52 237 5.13e-11 65.1
MS.gene34701.t1 AT5G56560 25.629 437 273 21 26 435 6 417 5.45e-11 64.7
MS.gene34701.t1 AT1G55660 32.161 199 111 11 24 211 52 237 6.27e-11 64.3
MS.gene34701.t1 AT4G09920 27.103 214 138 6 24 231 1 202 8.24e-11 63.5
MS.gene34701.t1 AT4G09920 27.103 214 138 6 24 231 1 202 8.24e-11 63.5
MS.gene34701.t1 AT1G55030 25.885 452 271 20 15 435 3 421 9.24e-11 63.9

Find 92 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACGAAGGATCTATCAATT+TGG 0.211457 8.1:-45723872 MS.gene34701:CDS
CTTTGGTCACAAAGTCTTTA+GGG 0.228938 8.1:+45723962 None:intergenic
CCCAAACCTGGAAACTTTGC+AGG 0.233508 8.1:-45724001 MS.gene34701:CDS
CCTGCAAAGTTTCCAGGTTT+GGG 0.262212 8.1:+45724001 None:intergenic
TGAAAACACTGCAGTCTTTA+CGG 0.271803 8.1:-45724138 MS.gene34701:intron
ACTTTGGTCACAAAGTCTTT+AGG 0.274506 8.1:+45723961 None:intergenic
ACCTGCAAAGTTTCCAGGTT+TGG 0.300500 8.1:+45724000 None:intergenic
GTAACTCACTATTGTCATTT+CGG 0.343521 8.1:+45723755 None:intergenic
TCGACAAATTTACTTTCAAC+TGG 0.344206 8.1:+45723796 None:intergenic
AGTAAACCTGCAAAGTTTCC+AGG 0.354081 8.1:+45723995 None:intergenic
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.1:-45723511 MS.gene34701:CDS
TTCGGATGAGGTTGAGAATA+GGG 0.359178 8.1:+45723773 None:intergenic
TCTTCTTCTTCTGCTTTAGC+TGG 0.361008 8.1:+45725180 None:intergenic
GAAATTGTCATTGGCTGTTT+CGG 0.367190 8.1:+45723915 None:intergenic
GCAAATATATGGTCGGGCTC+TGG 0.367759 8.1:-45723074 MS.gene34701:CDS
TAATCTCTGGAAGGATCTTC+AGG 0.371732 8.1:-45725019 MS.gene34701:CDS
TCACTGAAGAAGCTGGAAAT+TGG 0.372767 8.1:-45724072 MS.gene34701:CDS
CAAATATATGGTCGGGCTCT+GGG 0.377373 8.1:-45723073 MS.gene34701:CDS
TGTTGGCAACTTTCACAGTA+TGG 0.380596 8.1:-45723845 MS.gene34701:CDS
TATCACCTTCTTCATATTTG+AGG 0.384087 8.1:+45723722 None:intergenic
AACGAAGGATCTATCAATTT+GGG 0.387155 8.1:-45723871 MS.gene34701:CDS
CGACAGGTTAAGTAGTTTAC+CGG 0.389505 8.1:-45725124 MS.gene34701:CDS
TCTATCAATTTGGGTATTGT+TGG 0.395338 8.1:-45723862 MS.gene34701:CDS
TTGAAAGATTAGCAAATATA+TGG 0.400900 8.1:-45723085 MS.gene34701:CDS
GTCATTGGCTGTTTCGGAGC+TGG 0.401131 8.1:+45723921 None:intergenic
TTTGAATGTCGCGGGATTTG+CGG 0.402806 8.1:+45724923 None:intergenic
ACTGATCATGAAAAGACATT+TGG 0.423678 8.1:+45723045 None:intergenic
AAATCCTTCTATGTGAATAT+AGG 0.424387 8.1:+45723226 None:intergenic
CTGACCTATATTCACATAGA+AGG 0.433553 8.1:-45723230 MS.gene34701:CDS
CACTATTGTCATTTCGGATG+AGG 0.436761 8.1:+45723761 None:intergenic
CTCCTAGGTATCTTAATCTC+TGG 0.454079 8.1:-45725032 MS.gene34701:CDS
TCACTAACTTCGCCGTCGAC+AGG 0.472434 8.1:-45725140 MS.gene34701:CDS
TTTCGGATGAGGTTGAGAAT+AGG 0.478122 8.1:+45723772 None:intergenic
ACGTGGATCCTTGCTTCCAT+TGG 0.480869 8.1:-45724820 MS.gene34701:CDS
ATTCACATAGAAGGATTTCA+AGG 0.482694 8.1:-45723221 MS.gene34701:CDS
ATTTCCAGCTTCTTCAGTGA+TGG 0.484197 8.1:+45724075 None:intergenic
TCTCTCTAGCTCCCCAAACC+TGG 0.485407 8.1:-45724013 MS.gene34701:CDS
GTTTCGGAGCTGGACAAAGT+TGG 0.492761 8.1:+45723931 None:intergenic
ATATGACATATTAGCGAGTC+CGG 0.494354 8.1:+45725105 None:intergenic
CTTCTATGTGAATATAGGTC+AGG 0.500493 8.1:+45723231 None:intergenic
GATGGAAAATGAACAGAAGA+AGG 0.507493 8.1:+45724093 None:intergenic
TCCTTCGTTTATATTAAAGT+AGG 0.513346 8.1:+45723885 None:intergenic
CAAAGTTGGAACGAAAACTT+TGG 0.516256 8.1:+45723945 None:intergenic
GCATTGAGAAAGAAGGAAGC+CGG 0.516507 8.1:+45724748 None:intergenic
GGACCAAGTGAAATTGTCAT+TGG 0.520112 8.1:+45723906 None:intergenic
CATTGAGAAAGAAGGAAGCC+GGG 0.523422 8.1:+45724749 None:intergenic
AACAACCACGATTGAGAACA+TGG 0.529977 8.1:-45723295 MS.gene34701:CDS
ATTAGTAGCATTGAGAAAGA+AGG 0.531577 8.1:+45724741 None:intergenic
CGACAAATTTACTTTCAACT+GGG 0.532588 8.1:+45723797 None:intergenic
AGATTAGCAAATATATGGTC+GGG 0.538924 8.1:-45723080 MS.gene34701:CDS
TTGTTGAAGTTGAGTTCCAA+TGG 0.540180 8.1:+45724804 None:intergenic
CTACTAATATCGTTTGCCTC+AGG 0.540942 8.1:-45724726 MS.gene34701:intron
CAATGAGCCTTGTCTCTCCT+AGG 0.544772 8.1:-45725047 MS.gene34701:CDS
CTGCAAAGTTTCCAGGTTTG+GGG 0.545249 8.1:+45724002 None:intergenic
GCCGGGAGGTTAATTCGAAA+AGG 0.547695 8.1:+45724766 None:intergenic
GGAAATTGGCGCAGATATTG+TGG 0.550517 8.1:-45724058 MS.gene34701:CDS
AAAGGCCTCAAATATGAAGA+AGG 0.551811 8.1:-45723727 MS.gene34701:CDS
CATTTCTGAACGGAATTGAT+CGG 0.553482 8.1:+45724977 None:intergenic
AGAATTGACTTGAGATCGAA+AGG 0.553745 8.1:+45724849 None:intergenic
CAGCCAATGACAATTTCACT+TGG 0.554465 8.1:-45723909 MS.gene34701:CDS
AAGATTAGCAAATATATGGT+CGG 0.561234 8.1:-45723081 MS.gene34701:CDS
GGCTGCCATGTTCTCAATCG+TGG 0.563066 8.1:+45723290 None:intergenic
CATTTGCTGAATATATTAGA+CGG 0.563104 8.1:-45723181 MS.gene34701:CDS
GACAAATTTACTTTCAACTG+GGG 0.567559 8.1:+45723798 None:intergenic
GACAAGGCTCATTGTTGCGA+CGG 0.573921 8.1:+45725056 None:intergenic
GAATGTCGCGGGATTTGCGG+AGG 0.575959 8.1:+45724926 None:intergenic
TTCACATCATTCGACATCAA+AGG 0.576094 8.1:-45723689 MS.gene34701:intron
CTATGTGAATATAGGTCAGG+TGG 0.580589 8.1:+45723234 None:intergenic
TTGCTAATCTTTCAACAGAA+CGG 0.586267 8.1:+45723093 None:intergenic
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.1:+45723513 None:intergenic
GAAGAAGAATTCGACGACGG+AGG 0.591267 8.1:-45725165 MS.gene34701:CDS
AATAGTGAGTTACATCTCAA+AGG 0.593499 8.1:-45723745 MS.gene34701:CDS
ACGATTGAGAACATGGCAGC+CGG 0.595838 8.1:-45723288 MS.gene34701:CDS
TTACGGTGCCATCGTTATCG+AGG 0.597275 8.1:-45724121 MS.gene34701:CDS
AAATATATGGTCGGGCTCTG+GGG 0.605682 8.1:-45723072 MS.gene34701:CDS
TCCTACTTTAATATAAACGA+AGG 0.607865 8.1:-45723886 MS.gene34701:CDS
GTTGAGTTCCAATGGAAGCA+AGG 0.608223 8.1:+45724812 None:intergenic
GCTGAATATATTAGACGGAA+CGG 0.608350 8.1:-45723176 MS.gene34701:CDS
TTATGAGAACTTGAAGCATG+TGG 0.613522 8.1:+45723469 None:intergenic
TTCCAGAGATTAAGATACCT+AGG 0.613776 8.1:+45725030 None:intergenic
TAGGTATCTTAATCTCTGGA+AGG 0.622936 8.1:-45725028 MS.gene34701:CDS
TATGTGAATATAGGTCAGGT+GGG 0.640734 8.1:+45723235 None:intergenic
AATGATGCAACATTTCTGAA+CGG 0.642150 8.1:+45724967 None:intergenic
TAAACTACTTAACCTGTCGA+CGG 0.643627 8.1:+45725128 None:intergenic
TGTGGCATTTCTCAAGCACA+TGG 0.650003 8.1:+45723487 None:intergenic
TCTCATAATTAAGAGCAACA+AGG 0.667425 8.1:-45723454 MS.gene34701:intron
AAGTCAATTCTGTTGAAACG+TGG 0.669978 8.1:-45724837 MS.gene34701:CDS
TGGCAACTTTCACAGTATGG+CGG 0.688505 8.1:-45723842 MS.gene34701:CDS
TAAGATACCTAGGAGAGACA+AGG 0.699149 8.1:+45725040 None:intergenic
GAAGAAGAAGAATTCGACGA+CGG 0.706198 8.1:-45725168 MS.gene34701:CDS
AGGTTTCACCTCGATAACGA+TGG 0.724810 8.1:+45724113 None:intergenic
TGAGAAAGAAGGAAGCCGGG+AGG 0.751446 8.1:+45724752 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTGTTACAATT+TGG + chr8.1:45723858-45723877 None:intergenic 10.0%
!! ATCAACAATATTATAAGTAA+TGG + chr8.1:45723689-45723708 None:intergenic 15.0%
!! ATTACTTATAATATTGTTGA+TGG - chr8.1:45723688-45723707 MS.gene34701:intron 15.0%
!!! TGTTTTTTTTGATATTTCAA+TGG - chr8.1:45725100-45725119 MS.gene34701:CDS 15.0%
!! AATTGAATGTATCTAAAAAG+TGG - chr8.1:45724018-45724037 MS.gene34701:CDS 20.0%
!! AATTGAATGTATCTAAAAAG+TGG + chr8.1:45724037-45724018 None:intergenic 20.0%
!! AGTTTAATTCAAACAATCTT+TGG - chr8.1:45724818-45724837 MS.gene34701:CDS 20.0%
!! GTGGATAGAAAATAAATTAT+AGG + chr8.1:45723901-45723920 None:intergenic 20.0%
!! GTTTGAATTAAACTTGAAAA+CGG + chr8.1:45724812-45724831 None:intergenic 20.0%
!! TTGAAAGATTAGCAAATATA+TGG - chr8.1:45725147-45725166 MS.gene34701:CDS 20.0%
!! TTTATATTAGCTGTTGTATT+CGG - chr8.1:45723641-45723660 MS.gene34701:intron 20.0%
!!! ATACCATTACTCATTTTTTT+CGG + chr8.1:45723813-45723832 None:intergenic 20.0%
!!! TATGTTTTGTATGTTTAGTT+TGG - chr8.1:45724587-45724606 MS.gene34701:intron 20.0%
! AAATCCTTCTATGTGAATAT+AGG + chr8.1:45725009-45725028 None:intergenic 25.0%
! AAGATTAGCAAATATATGGT+CGG - chr8.1:45725151-45725170 MS.gene34701:CDS 25.0%
! AATATAAACGCTTTCGAATT+TGG + chr8.1:45723629-45723648 None:intergenic 25.0%
! ACAAAAAAAAAAAACACTGC+AGG - chr8.1:45723865-45723884 MS.gene34701:CDS 25.0%
! CATTCAATTAATGATGTATG+TGG - chr8.1:45723968-45723987 MS.gene34701:CDS 25.0%
! TCCTACTTTAATATAAACGA+AGG - chr8.1:45724346-45724365 MS.gene34701:intron 25.0%
! TCCTTCGTTTATATTAAAGT+AGG + chr8.1:45724350-45724369 None:intergenic 25.0%
! TGTGATGATAGCATTAAAAT+AGG + chr8.1:45723540-45723559 None:intergenic 25.0%
!! AATTCAGGATAATTTAGAAC+AGG + chr8.1:45724668-45724687 None:intergenic 25.0%
!! ATTCAGGATAATTTAGAACA+GGG + chr8.1:45724667-45724686 None:intergenic 25.0%
!! CATTTGCTGAATATATTAGA+CGG - chr8.1:45725051-45725070 MS.gene34701:CDS 25.0%
!! TTTTTGCTTATATTAGTGAC+CGG - chr8.1:45724042-45724061 MS.gene34701:CDS 25.0%
!! TTTTTGCTTATATTAGTGAC+CGG + chr8.1:45724061-45724042 None:intergenic 25.0%
!!! TAAGCACATTTTAACAAAGT+TGG - chr8.1:45723787-45723806 MS.gene34701:CDS 25.0%
AAACGAAGGATCTATCAATT+TGG - chr8.1:45724360-45724379 MS.gene34701:intron 30.0%
AACGAAGGATCTATCAATTT+GGG - chr8.1:45724361-45724380 MS.gene34701:intron 30.0%
AATAGTGAGTTACATCTCAA+AGG - chr8.1:45724487-45724506 MS.gene34701:intron 30.0%
AATGATGCAACATTTCTGAA+CGG + chr8.1:45723268-45723287 None:intergenic 30.0%
ACTGATCATGAAAAGACATT+TGG + chr8.1:45725190-45725209 None:intergenic 30.0%
AGATTAGCAAATATATGGTC+GGG - chr8.1:45725152-45725171 MS.gene34701:CDS 30.0%
ATGTTTCAACTCAAATCTTC+TGG - chr8.1:45724721-45724740 MS.gene34701:intron 30.0%
ATTCACATAGAAGGATTTCA+AGG - chr8.1:45725011-45725030 MS.gene34701:CDS 30.0%
CGACAAATTTACTTTCAACT+GGG + chr8.1:45724438-45724457 None:intergenic 30.0%
CTTATGAATGACATCAAACA+TGG + chr8.1:45723771-45723790 None:intergenic 30.0%
GACAAATTTACTTTCAACTG+GGG + chr8.1:45724437-45724456 None:intergenic 30.0%
TATCACCTTCTTCATATTTG+AGG + chr8.1:45724513-45724532 None:intergenic 30.0%
TCGACAAATTTACTTTCAAC+TGG + chr8.1:45724439-45724458 None:intergenic 30.0%
TCTCATAATTAAGAGCAACA+AGG - chr8.1:45724778-45724797 MS.gene34701:CDS 30.0%
TGATGTAAATCACGAAATTC+AGG + chr8.1:45724683-45724702 None:intergenic 30.0%
TTGCTAATCTTTCAACAGAA+CGG + chr8.1:45725142-45725161 None:intergenic 30.0%
! ATTAGTAGCATTGAGAAAGA+AGG + chr8.1:45723494-45723513 None:intergenic 30.0%
! GTAACTCACTATTGTCATTT+CGG + chr8.1:45724480-45724499 None:intergenic 30.0%
! TCTATCAATTTGGGTATTGT+TGG - chr8.1:45724370-45724389 MS.gene34701:intron 30.0%
! TTCAGGATAATTTAGAACAG+GGG + chr8.1:45724666-45724685 None:intergenic 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG + chr8.1:45724722-45724741 None:intergenic 30.0%
AAAATAGGTTAACGTACCTG+AGG + chr8.1:45723525-45723544 None:intergenic 35.0%
AAAGGCCTCAAATATGAAGA+AGG - chr8.1:45724505-45724524 MS.gene34701:intron 35.0%
AAGTCAATTCTGTTGAAACG+TGG - chr8.1:45723395-45723414 MS.gene34701:intron 35.0%
ACTTTGGTCACAAAGTCTTT+AGG + chr8.1:45724274-45724293 None:intergenic 35.0%
ATATGACATATTAGCGAGTC+CGG + chr8.1:45723130-45723149 None:intergenic 35.0%
CAAAGTTGGAACGAAAACTT+TGG + chr8.1:45724290-45724309 None:intergenic 35.0%
CATTTCTGAACGGAATTGAT+CGG + chr8.1:45723258-45723277 None:intergenic 35.0%
CTGACCTATATTCACATAGA+AGG - chr8.1:45725002-45725021 MS.gene34701:CDS 35.0%
CTTCTATGTGAATATAGGTC+AGG + chr8.1:45725004-45725023 None:intergenic 35.0%
CTTTGGTCACAAAGTCTTTA+GGG + chr8.1:45724273-45724292 None:intergenic 35.0%
GATGGAAAATGAACAGAAGA+AGG + chr8.1:45724142-45724161 None:intergenic 35.0%
GCTGAATATATTAGACGGAA+CGG - chr8.1:45725056-45725075 MS.gene34701:CDS 35.0%
TAAACTACTTAACCTGTCGA+CGG + chr8.1:45723107-45723126 None:intergenic 35.0%
TAGGTATCTTAATCTCTGGA+AGG - chr8.1:45723204-45723223 MS.gene34701:CDS 35.0%
TATGTGAATATAGGTCAGGT+GGG + chr8.1:45725000-45725019 None:intergenic 35.0%
TGAAAACACTGCAGTCTTTA+CGG - chr8.1:45724094-45724113 MS.gene34701:CDS 35.0%
TGGCCGAAAAAAATGAGTAA+TGG - chr8.1:45723807-45723826 MS.gene34701:CDS 35.0%
TTATGAGAACTTGAAGCATG+TGG + chr8.1:45724766-45724785 None:intergenic 35.0%
TTCACATCATTCGACATCAA+AGG - chr8.1:45724543-45724562 MS.gene34701:intron 35.0%
TTCCAGAGATTAAGATACCT+AGG + chr8.1:45723205-45723224 None:intergenic 35.0%
TTGTTGAAGTTGAGTTCCAA+TGG + chr8.1:45723431-45723450 None:intergenic 35.0%
! AAGTTAAAAAGTACACCCTC+CGG + chr8.1:45724064-45724083 None:intergenic 35.0%
! GAAATTGTCATTGGCTGTTT+CGG + chr8.1:45724320-45724339 None:intergenic 35.0%
!! AGAATTGACTTGAGATCGAA+AGG + chr8.1:45723386-45723405 None:intergenic 35.0%
!! TCAAAAACTGTTGTTGACTC+CGG + chr8.1:45724966-45724985 None:intergenic 35.0%
!!! ATGAAAGTTTTGAATGTCGC+GGG + chr8.1:45723320-45723339 None:intergenic 35.0%
!!! GATGAAAGTTTTGAATGTCG+CGG + chr8.1:45723321-45723340 None:intergenic 35.0%
AACAACCACGATTGAGAACA+TGG - chr8.1:45724937-45724956 MS.gene34701:CDS 40.0%
AGTAAACCTGCAAAGTTTCC+AGG + chr8.1:45724240-45724259 None:intergenic 40.0%
ATTTCCAGCTTCTTCAGTGA+TGG + chr8.1:45724160-45724179 None:intergenic 40.0%
CAGCCAATGACAATTTCACT+TGG - chr8.1:45724323-45724342 MS.gene34701:intron 40.0%
CGACAGGTTAAGTAGTTTAC+CGG - chr8.1:45723108-45723127 MS.gene34701:CDS 40.0%
CTACTAATATCGTTTGCCTC+AGG - chr8.1:45723506-45723525 MS.gene34701:CDS 40.0%
CTATGTGAATATAGGTCAGG+TGG + chr8.1:45725001-45725020 None:intergenic 40.0%
CTCCTAGGTATCTTAATCTC+TGG - chr8.1:45723200-45723219 MS.gene34701:CDS 40.0%
GAAGAAGAAGAATTCGACGA+CGG - chr8.1:45723064-45723083 MS.gene34701:CDS 40.0%
GGACCAAGTGAAATTGTCAT+TGG + chr8.1:45724329-45724348 None:intergenic 40.0%
TAAGATACCTAGGAGAGACA+AGG + chr8.1:45723195-45723214 None:intergenic 40.0%
TCACTGAAGAAGCTGGAAAT+TGG - chr8.1:45724160-45724179 MS.gene34701:intron 40.0%
TCTTCTTCTTCTGCTTTAGC+TGG + chr8.1:45723055-45723074 None:intergenic 40.0%
TGCTTATATTAGTGACCGGA+GGG - chr8.1:45724046-45724065 MS.gene34701:CDS 40.0%
TGCTTATATTAGTGACCGGA+GGG + chr8.1:45724065-45724046 None:intergenic 40.0%
TGTTGGCAACTTTCACAGTA+TGG - chr8.1:45724387-45724406 MS.gene34701:intron 40.0%
TTCGGATGAGGTTGAGAATA+GGG + chr8.1:45724462-45724481 None:intergenic 40.0%
TTGCTTATATTAGTGACCGG+AGG - chr8.1:45724045-45724064 MS.gene34701:CDS 40.0%
TTGCTTATATTAGTGACCGG+AGG + chr8.1:45724064-45724045 None:intergenic 40.0%
TTTCGGATGAGGTTGAGAAT+AGG + chr8.1:45724463-45724482 None:intergenic 40.0%
! CACTATTGTCATTTCGGATG+AGG + chr8.1:45724474-45724493 None:intergenic 40.0%
! TAATCTCTGGAAGGATCTTC+AGG - chr8.1:45723213-45723232 MS.gene34701:CDS 40.0%
! TAATTTAGAACAGGGGCATG+AGG + chr8.1:45724659-45724678 None:intergenic 40.0%
! TCCTTTTCGAATTAACCTCC+CGG - chr8.1:45723465-45723484 MS.gene34701:CDS 40.0%
! TTTTCCATCACTGAAGAAGC+TGG - chr8.1:45724153-45724172 MS.gene34701:intron 40.0%
!!! TTGCGACGGAAGTTTTAGTT+GGG + chr8.1:45723165-45723184 None:intergenic 40.0%
AAATATATGGTCGGGCTCTG+GGG - chr8.1:45725160-45725179 MS.gene34701:CDS 45.0%
ACCTGCAAAGTTTCCAGGTT+TGG + chr8.1:45724235-45724254 None:intergenic 45.0%
AGGTTTCACCTCGATAACGA+TGG + chr8.1:45724122-45724141 None:intergenic 45.0%
CAAATATATGGTCGGGCTCT+GGG - chr8.1:45725159-45725178 MS.gene34701:CDS 45.0%
CATTGAGAAAGAAGGAAGCC+GGG + chr8.1:45723486-45723505 None:intergenic 45.0%
CCTGCAAAGTTTCCAGGTTT+GGG + chr8.1:45724234-45724253 None:intergenic 45.0%
CTGCAAAGTTTCCAGGTTTG+GGG + chr8.1:45724233-45724252 None:intergenic 45.0%
GCATTGAGAAAGAAGGAAGC+CGG + chr8.1:45723487-45723506 None:intergenic 45.0%
GGAAATTGGCGCAGATATTG+TGG - chr8.1:45724174-45724193 MS.gene34701:intron 45.0%
GTTCCTCCTACCAGCAAAAA+GGG + chr8.1:45724920-45724939 None:intergenic 45.0%
GTTGAGTTCCAATGGAAGCA+AGG + chr8.1:45723423-45723442 None:intergenic 45.0%
TGGCAACTTTCACAGTATGG+CGG - chr8.1:45724390-45724409 MS.gene34701:intron 45.0%
TGTGGCATTTCTCAAGCACA+TGG + chr8.1:45724748-45724767 None:intergenic 45.0%
TGTTCCTCCTACCAGCAAAA+AGG + chr8.1:45724921-45724940 None:intergenic 45.0%
TTCTATCCACTTACACCCTC+CGG - chr8.1:45723911-45723930 MS.gene34701:CDS 45.0%
TTGTTATCGTGTACGTGCAG+TGG - chr8.1:45724630-45724649 MS.gene34701:intron 45.0%
TTTGAATGTCGCGGGATTTG+CGG + chr8.1:45723312-45723331 None:intergenic 45.0%
! GACATGATGTCCCTTTTTGC+TGG - chr8.1:45724907-45724926 MS.gene34701:CDS 45.0%
! TGATGTCCCTTTTTGCTGGT+AGG - chr8.1:45724911-45724930 MS.gene34701:CDS 45.0%
!! GTTGCGACGGAAGTTTTAGT+TGG + chr8.1:45723166-45723185 None:intergenic 45.0%
ACGATTGAGAACATGGCAGC+CGG - chr8.1:45724944-45724963 MS.gene34701:CDS 50.0%
ACGTGGATCCTTGCTTCCAT+TGG - chr8.1:45723412-45723431 MS.gene34701:intron 50.0%
CCCAAACCTGGAAACTTTGC+AGG - chr8.1:45724231-45724250 MS.gene34701:intron 50.0%
GAAGAAGAATTCGACGACGG+AGG - chr8.1:45723067-45723086 MS.gene34701:CDS 50.0%
GACAAGGCTCATTGTTGCGA+CGG + chr8.1:45723179-45723198 None:intergenic 50.0%
GCAAATATATGGTCGGGCTC+TGG - chr8.1:45725158-45725177 MS.gene34701:CDS 50.0%
GCCGGGAGGTTAATTCGAAA+AGG + chr8.1:45723469-45723488 None:intergenic 50.0%
GTTTCGGAGCTGGACAAAGT+TGG + chr8.1:45724304-45724323 None:intergenic 50.0%
! CAATGAGCCTTGTCTCTCCT+AGG - chr8.1:45723185-45723204 MS.gene34701:CDS 50.0%
! TGTCCCTTTTTGCTGGTAGG+AGG - chr8.1:45724914-45724933 MS.gene34701:CDS 50.0%
! TTTAGAACAGGGGCATGAGG+TGG + chr8.1:45724656-45724675 None:intergenic 50.0%
!! TTACGGTGCCATCGTTATCG+AGG - chr8.1:45724111-45724130 MS.gene34701:CDS 50.0%
!!! CGACGGAAGTTTTAGTTGGG+AGG + chr8.1:45723162-45723181 None:intergenic 50.0%
GGCTGCCATGTTCTCAATCG+TGG + chr8.1:45724945-45724964 None:intergenic 55.0%
TCACTAACTTCGCCGTCGAC+AGG - chr8.1:45723092-45723111 MS.gene34701:CDS 55.0%
TGAGAAAGAAGGAAGCCGGG+AGG + chr8.1:45723483-45723502 None:intergenic 55.0%
! GTCATTGGCTGTTTCGGAGC+TGG + chr8.1:45724314-45724333 None:intergenic 55.0%
! TAGTGACCGGAGGGTGTAAG+TGG + chr8.1:45723920-45723939 None:intergenic 55.0%
! TCTCTCTAGCTCCCCAAACC+TGG - chr8.1:45724219-45724238 MS.gene34701:intron 55.0%
GAATGTCGCGGGATTTGCGG+AGG + chr8.1:45723309-45723328 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 45723037 45725217 45723037 ID=MS.gene34701
chr8.1 mRNA 45723037 45725217 45723037 ID=MS.gene34701.t1;Parent=MS.gene34701
chr8.1 exon 45724727 45725217 45724727 ID=MS.gene34701.t1.exon1;Parent=MS.gene34701.t1
chr8.1 CDS 45724727 45725217 45724727 ID=cds.MS.gene34701.t1;Parent=MS.gene34701.t1
chr8.1 exon 45723690 45724146 45723690 ID=MS.gene34701.t1.exon2;Parent=MS.gene34701.t1
chr8.1 CDS 45723690 45724146 45723690 ID=cds.MS.gene34701.t1;Parent=MS.gene34701.t1
chr8.1 exon 45723455 45723544 45723455 ID=MS.gene34701.t1.exon3;Parent=MS.gene34701.t1
chr8.1 CDS 45723455 45723544 45723455 ID=cds.MS.gene34701.t1;Parent=MS.gene34701.t1
chr8.1 exon 45723037 45723321 45723037 ID=MS.gene34701.t1.exon4;Parent=MS.gene34701.t1
chr8.1 CDS 45723037 45723321 45723037 ID=cds.MS.gene34701.t1;Parent=MS.gene34701.t1
Gene Sequence

>MS.gene34701

ATGGCGGAGATGAAACCAGCTAAAGCAGAAGAAGAAGAATTCGACGACGGAGGAGTCACTAACTTCGCCGTCGACAGGTTAAGTAGTTTACCGGACTCGCTAATATGTCATATAATGTCATTCCTCCCAACTAAAACTTCCGTCGCAACAATGAGCCTTGTCTCTCCTAGGTATCTTAATCTCTGGAAGGATCTTCAGGTCTTCTATTTCAACTTTCACCGATCAATTCCGTTCAGAAATGTTGCATCATTCGTAAACTCTGTTCTCATCCTCCGCAAATCCCGCGACATTCAAAACTTTCATCTCACTTTCAATTGTGATCGATTCAAGCAATTGAATTTTGAAGCCTTTCGATCTCAAGTCAATTCTGTTGAAACGTGGATCCTTGCTTCCATTGGAACTCAACTTCAACAACTCTTTCTCGATGTTCCTTTTCGAATTAACCTCCCGGCTTCCTTCTTTCTCAATGCTACTAATATCGTTTGCCTCAGGTACGTTAACCTATTTTAATGCTATCATCACACACACAGACATACAAGTTATTATCACTAACGTTAACAAGTTAGTGTTTAAGTTTCTTTTTTTCGTTCCAAATTCGAAAGCGTTTATATTAGCTGTTGTATTCGGTTTGATTTGAAACAAAATTGTTCCATTACTTATAATATTGTTGATGGAATATTTATCTGTAAAATTATGTTTGAATTCAAAAACAAGAAAATGTGCGTAAATAACCATGTTTGATGTCATTCATAAGCACATTTTAACAAAGTTGGCCGAAAAAAATGAGTAATGGTATAAATATTCTATGATTTAAAAGACCAAATTGTAACAAAAAAAAAAAACACTGCAGGAGACATTTGACCTATAATTTATTTTCTATCCACTTACACCCTCCGGTCACTAATATAAGCAAAAATCCACTTTTTAGATACATTCAATTAATGATGTATGTGGTCTTTATTATATGTCACATACATTATTAATTGAATGTATCTAAAAAGTGGATTTTTGCTTATATTAGTGACCGGAGGGTGTACTTTTTAACTTATCTGACTTTTGAAAACACTGCAGTCTTTACGGTGCCATCGTTATCGAGGTGAAACCTTCTTCTGTTCATTTTCCATCACTGAAGAAGCTGGAAATTGGCGCAGATATTGTGGATGTAAATGAAATAGTTTACTTTCTCTCTAGCTCCCCAAACCTGGAAACTTTGCAGGTTTACTTTTACTTTGTCCCTAAAGACTTTGTGACCAAAGTTTTCGTTCCAACTTTGTCCAGCTCCGAAACAGCCAATGACAATTTCACTTGGTCCTACTTTAATATAAACGAAGGATCTATCAATTTGGGTATTGTTGGCAACTTTCACAGTATGGCGGAAGCGTTTCTTGATGTATTTTCCCCAGTTGAAAGTAAATTTGTCGACCCTATTCTCAACCTCATCCGAAATGACAATAGTGAGTTACATCTCAAAGGCCTCAAATATGAAGAAGGTGATATATATCTACTTTCACATCATTCGACATCAAAGGTGAGTTTTATTTTTATAGTTATATGTTTTGTATGTTTAGTTTGGTACTAATATTGCATCATCAATTGTTATCGTGTACGTGCAGTGGCCACCTCATGCCCCTGTTCTAAATTATCCTGAATTTCGTGATTTACATCATCTAAAGTTCGTTCTTCCATGTTTCAACTCAAATCTTCTGGTCCATGTGCTTGAGAAATGCCACATGCTTCAAGTTCTCATAATTAAGAGCAACAAGGTTTGTTGACCGTTTTCAAGTTTAATTCAAACAATCTTTGGTCTATTTATATATACATGCATGCATGTCTCTCTATATCTGTACACACAAATATGTTTTTGAGTCATGACATGATGTCCCTTTTTGCTGGTAGGAGGAACAACCACGATTGAGAACATGGCAGCCGGAGTCAACAACAGTTTTTGAATGTCTCAAATCCCACCTGACCTATATTCACATAGAAGGATTTCAAGGACATGAAGATGAGCTGACATTTGCTGAATATATTAGACGGAACGGACTTGTTTTGAAGACAATGCTTGTTTTTTTTGATATTTCAATGGATAAAACGAACAAGTACCGTTCTGTTGAAAGATTAGCAAATATATGGTCGGGCTCTGGGGCATGCCAAATGTCTTTTCATGATCAGTCTTACTGA
Protein sequence

>MS.gene34701.t1

MAEMKPAKAEEEEFDDGGVTNFAVDRLSSLPDSLICHIMSFLPTKTSVATMSLVSPRYLNLWKDLQVFYFNFHRSIPFRNVASFVNSVLILRKSRDIQNFHLTFNCDRFKQLNFEAFRSQVNSVETWILASIGTQLQQLFLDVPFRINLPASFFLNATNIVCLSLYGAIVIEVKPSSVHFPSLKKLEIGADIVDVNEIVYFLSSSPNLETLQVYFYFVPKDFVTKVFVPTLSSSETANDNFTWSYFNINEGSINLGIVGNFHSMAEAFLDVFSPVESKFVDPILNLIRNDNSELHLKGLKYEEGDIYLLSHHSTSKFVLPCFNSNLLVHVLEKCHMLQVLIIKSNKEEQPRLRTWQPESTTVFECLKSHLTYIHIEGFQGHEDELTFAEYIRRNGLVLKTMLVFFDISMDKTNKYRSVERLANIWSGSGACQMSFHDQSY