AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36754

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36754.t1 MTR_8g014260 48.359 457 109 11 1 336 1 451 9.23e-116 342
MS.gene36754.t1 MTR_4g024710 53.242 293 82 4 96 336 58 347 1.54e-87 267
MS.gene36754.t1 MTR_4g024745 53.480 273 72 4 99 319 15 284 1.61e-82 252
MS.gene36754.t1 MTR_8g445780 40.741 405 107 8 2 290 3 390 3.26e-78 244
MS.gene36754.t1 MTR_8g077460 40.295 407 140 10 1 336 1 375 7.26e-76 238
MS.gene36754.t1 MTR_8g014160 36.364 396 173 11 12 336 38 425 3.49e-63 207
MS.gene36754.t1 MTR_8g014210 50.216 231 63 2 157 336 2 231 1.29e-62 199
MS.gene36754.t1 MTR_5g092960 32.222 450 169 13 15 335 19 461 6.79e-52 178
MS.gene36754.t1 MTR_2g083840 31.020 461 174 15 7 336 10 457 2.08e-47 166
MS.gene36754.t1 MTR_8g468760 31.555 431 186 14 1 335 1 418 2.21e-47 165
MS.gene36754.t1 MTR_8g062270 31.742 419 165 13 29 335 44 453 3.17e-44 157
MS.gene36754.t1 MTR_8g468840 32.787 427 162 11 1 307 2 423 3.41e-44 157
MS.gene36754.t1 MTR_8g467800 31.670 461 173 16 3 336 8 453 1.82e-41 150
MS.gene36754.t1 MTR_8g468810 29.004 462 191 12 2 336 12 463 2.72e-39 144
MS.gene36754.t1 MTR_8g468810 31.635 373 168 10 2 304 12 367 5.16e-38 139
MS.gene36754.t1 MTR_8g070950 30.088 452 182 17 1 336 1 434 8.05e-37 137
MS.gene36754.t1 MTR_8g468810 31.908 304 138 6 2 242 12 309 1.06e-34 129
MS.gene36754.t1 MTR_8g468810 31.908 304 138 6 2 242 12 309 1.27e-34 129
MS.gene36754.t1 MTR_8g468810 31.908 304 138 6 2 242 12 309 1.71e-34 129
MS.gene36754.t1 MTR_5g092960 32.181 376 168 11 1 303 5 366 3.67e-34 129
MS.gene36754.t1 MTR_8g469020 28.414 454 184 16 15 336 7 451 7.75e-34 129
MS.gene36754.t1 MTR_8g014230 65.909 88 29 1 249 336 18 104 4.01e-33 119
MS.gene36754.t1 MTR_8g058970 33.555 301 133 9 99 336 181 477 1.53e-32 126
MS.gene36754.t1 MTR_8g058965 28.132 455 183 16 15 336 13 456 2.02e-31 123
MS.gene36754.t1 MTR_8g467800 34.286 315 126 12 3 249 8 309 2.86e-30 118
MS.gene36754.t1 MTR_4g032730 28.870 478 185 18 1 335 1 466 8.59e-29 115
MS.gene36754.t1 MTR_8g062010 29.062 437 192 19 9 336 3 430 1.07e-27 112
MS.gene36754.t1 MTR_8g062310 28.538 424 181 17 15 336 25 428 5.47e-27 110
MS.gene36754.t1 MTR_2g086020 29.738 343 175 14 29 336 4 315 6.81e-20 89.0
MS.gene36754.t1 MTR_8g014290 42.222 135 57 5 13 128 8 140 1.89e-18 81.6
MS.gene36754.t1 MTR_5g021550 40.157 127 60 3 10 121 14 139 1.54e-17 79.3
MS.gene36754.t1 MTR_4g024720 44.068 118 44 5 1 100 1 114 4.26e-16 76.3
MS.gene36754.t1 MTR_8g062080 44.086 93 50 2 246 336 353 445 5.22e-16 79.0
MS.gene36754.t1 MTR_8g062300 39.785 93 54 2 246 336 385 477 2.18e-13 71.2
MS.gene36754.t1 MTR_4g024770 50.000 66 17 2 1 50 8 73 3.62e-13 64.7
MS.gene36754.t1 MTR_5g012840 26.284 331 204 12 23 336 11 318 1.65e-11 64.7
MS.gene36754.t1 MTR_0543s0010 24.031 387 207 12 14 335 85 449 3.70e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36754.t1 AT3G26920 26.141 241 156 8 111 335 66 300 1.10e-11 65.1

Find 75 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGACCTTAATTCAATTTAA+AGG 0.153246 8.2:-78777929 MS.gene36754:CDS
GCAATTTGAAATCCATTCTT+TGG 0.213271 8.2:-78778859 MS.gene36754:CDS
AATCCATTCTTTGGGTTATC+TGG 0.229805 8.2:-78778850 MS.gene36754:CDS
ATTCAATTTAAAGGATTTCT+TGG 0.243436 8.2:-78777920 MS.gene36754:CDS
AAACATCACTGAATTTGTTT+TGG 0.271901 8.2:+78778637 None:intergenic
TGTTTGTTCCTCAGTATCTT+TGG 0.274451 8.2:-78778942 MS.gene36754:intron
CTCCTCGGCGCTCTCTCTGC+TGG 0.295657 8.2:-78778528 MS.gene36754:CDS
GGCAAACTTTGTCTGAGCTT+TGG 0.296523 8.2:+78778757 None:intergenic
CTCGTCGTTGGCGACACCTT+TGG 0.298239 8.2:-78779505 MS.gene36754:CDS
AAATCCTTTAAATTGAATTA+AGG 0.307099 8.2:+78777925 None:intergenic
AGATGGGATATAAGACATTT+AGG 0.327868 8.2:+78777950 None:intergenic
CTGCTGGGACAAAACTTCTA+TGG 0.357303 8.2:-78778512 MS.gene36754:CDS
GATCTCCTTCCCTCGTCGTT+TGG 0.365317 8.2:-78778573 MS.gene36754:CDS
CAATTTGAAATCCATTCTTT+GGG 0.370180 8.2:-78778858 MS.gene36754:CDS
ACTGAAAAGCTTCAATGCTT+TGG 0.390192 8.2:-78779414 MS.gene36754:CDS
ATCGTTATTAAACATGAAGT+TGG 0.391412 8.2:-78778708 MS.gene36754:CDS
TCAATAAACCCCGCGTCATT+CGG 0.391921 8.2:-78779370 MS.gene36754:CDS
GAATATTGTACTTCACCATT+AGG 0.396744 8.2:+78777810 None:intergenic
CGCCGAGGAGTTTGACTAAT+GGG 0.400065 8.2:+78778541 None:intergenic
TGACGATTCCTTCAACTTCA+AGG 0.400990 8.2:-78779456 MS.gene36754:CDS
TGTGGAGCCCATCCTAAAAT+TGG 0.402676 8.2:+78777983 None:intergenic
TGATATTTCTGTGGACCTAA+TGG 0.409765 8.2:-78777825 MS.gene36754:CDS
TCCTCGGCGCTCTCTCTGCT+GGG 0.433017 8.2:-78778527 MS.gene36754:CDS
GAATGACGCGGGGTTTATTG+AGG 0.460268 8.2:+78779372 None:intergenic
TAAATTGAATTAAGGTCAGA+TGG 0.470814 8.2:+78777933 None:intergenic
TTGGAAGAATATCCAGGTGC+TGG 0.470851 8.2:-78779486 MS.gene36754:CDS
GGGGTTTATTGAGGAGGGCT+AGG 0.471828 8.2:+78779381 None:intergenic
GCGCCGAGGAGTTTGACTAA+TGG 0.477807 8.2:+78778540 None:intergenic
GCCTCCCACCTACCTTGAAA+AGG 0.482061 8.2:-78778737 MS.gene36754:CDS
TCGTTATTAAACATGAAGTT+GGG 0.495214 8.2:-78778707 MS.gene36754:CDS
TGCAAGTTGGCACATTATTG+AGG 0.496526 8.2:+78777760 None:intergenic
CGTCATAGAAATCCAGCACC+TGG 0.502939 8.2:+78779474 None:intergenic
CTTCCAGATAACCCAAAGAA+TGG 0.505197 8.2:+78778847 None:intergenic
AGAGCCATCCTTGAAGTTGA+AGG 0.506067 8.2:+78779448 None:intergenic
TCAACAAATGACACCTCATC+TGG 0.509197 8.2:+78777893 None:intergenic
GGGACAAAACTTCTATGGTT+GGG 0.523080 8.2:-78778507 MS.gene36754:CDS
ACACCTTTGGAAGAATATCC+AGG 0.532492 8.2:-78779492 MS.gene36754:CDS
CGGAACCAAACGACGAGGGA+AGG 0.540506 8.2:+78778568 None:intergenic
TGGGACAAAACTTCTATGGT+TGG 0.544136 8.2:-78778508 MS.gene36754:CDS
CGGAAGATGCTTCTCCATTG+TGG 0.555578 8.2:-78779350 MS.gene36754:CDS
CGTCTGGTAATCTACTGATC+CGG 0.560242 8.2:+78779594 None:intergenic
ATGCTAAGGAACTTAAGATC+GGG 0.560763 8.2:+78778663 None:intergenic
AATGCTAAGGAACTTAAGAT+CGG 0.564956 8.2:+78778662 None:intergenic
GATTCCTTCAACTTCAAGGA+TGG 0.569100 8.2:-78779452 MS.gene36754:CDS
AAGCATCTTCCGAATGACGC+GGG 0.570682 8.2:+78779361 None:intergenic
ATAAGACATTTAGGAACACT+TGG 0.572853 8.2:+78777959 None:intergenic
CATTTAGGAACACTTGGCTG+TGG 0.572984 8.2:+78777965 None:intergenic
TGACGCGGGGTTTATTGAGG+AGG 0.578009 8.2:+78779375 None:intergenic
GATTATTGCTGATATTTCTG+TGG 0.583156 8.2:-78777834 MS.gene36754:CDS
TCTTCTGTGAATGCAATCCT+CGG 0.583937 8.2:-78778822 MS.gene36754:CDS
AAATTGAATTAAGGTCAGAT+GGG 0.585897 8.2:+78777934 None:intergenic
CAACTCAGTCAAATGCAAGT+TGG 0.587010 8.2:+78777747 None:intergenic
CCGGCTCTCTATCTCGTCGT+TGG 0.587843 8.2:-78779517 MS.gene36754:CDS
ACCAAAACAGTGGTTACCAC+CGG 0.592718 8.2:-78779536 MS.gene36754:intron
AAGATTATCGAAAGTGCGAA+GGG 0.595018 8.2:-78777783 MS.gene36754:CDS
GTCCCATTAGTCAAACTCCT+CGG 0.598203 8.2:-78778543 MS.gene36754:CDS
AATTGCAGCAGCTTCAGTGA+TGG 0.598966 8.2:+78778876 None:intergenic
GCACCTGGATATTCTTCCAA+AGG 0.599770 8.2:+78779489 None:intergenic
GAAGATTATCGAAAGTGCGA+AGG 0.605762 8.2:-78777784 MS.gene36754:CDS
ATTTCTTGGATTACCAGATG+AGG 0.609098 8.2:-78777906 MS.gene36754:CDS
GACGCGGGGTTTATTGAGGA+GGG 0.611529 8.2:+78779376 None:intergenic
AGCATCTTCCGAATGACGCG+GGG 0.634827 8.2:+78779362 None:intergenic
CGAAAATATAGTGACCACAA+TGG 0.641302 8.2:+78779336 None:intergenic
ACGACGGAACCAAACGACGA+GGG 0.667009 8.2:+78778564 None:intergenic
GCACAACTTGCACAACGTGG+TGG 0.668875 8.2:-78778607 MS.gene36754:CDS
ACGACGAGATAGAGAGCCGG+TGG 0.680836 8.2:+78779520 None:intergenic
GAAGCATCTTCCGAATGACG+CGG 0.686092 8.2:+78779360 None:intergenic
TCCCAGCAGAGAGAGCGCCG+AGG 0.686302 8.2:+78778526 None:intergenic
GTTGGGTGCTTCAACAACCA+AGG 0.688558 8.2:-78778490 MS.gene36754:intron
CCAACGACGAGATAGAGAGC+CGG 0.691032 8.2:+78779517 None:intergenic
GACGACGGAACCAAACGACG+AGG 0.696892 8.2:+78778563 None:intergenic
GAGTTTGACTAATGGGACGA+CGG 0.698443 8.2:+78778548 None:intergenic
CATGCACAACTTGCACAACG+TGG 0.698471 8.2:-78778610 MS.gene36754:CDS
CGGATCAGTAGATTACCAGA+CGG 0.724138 8.2:-78779593 MS.gene36754:intron
TCTGTGAATGCAATCCTCGG+CGG 0.745793 8.2:-78778819 MS.gene36754:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAATAAATAAATA+GGG + chr8.2:78778235-78778254 None:intergenic 0.0%
!! AAATCCTTTAAATTGAATTA+AGG + chr8.2:78779467-78779486 None:intergenic 15.0%
!!! AATAGTGAAATTTTTGTAAA+AGG + chr8.2:78779081-78779100 None:intergenic 15.0%
!! ATTCAATTTAAAGGATTTCT+TGG - chr8.2:78779469-78779488 MS.gene36754:CDS 20.0%
!! GTAATCTATTTCATAAAAAC+TGG + chr8.2:78779270-78779289 None:intergenic 20.0%
!! TCATGATTTACTTATGTATA+TGG - chr8.2:78778980-78778999 MS.gene36754:intron 20.0%
!!! AGATCCAAAATTTCAATTTT+TGG + chr8.2:78778600-78778619 None:intergenic 20.0%
!!! GATCCAAAATTTCAATTTTT+GGG + chr8.2:78778599-78778618 None:intergenic 20.0%
! AAATTGAATTAAGGTCAGAT+GGG + chr8.2:78779458-78779477 None:intergenic 25.0%
! ACTAAAATAAAACTTGACCT+TGG + chr8.2:78778919-78778938 None:intergenic 25.0%
! ATCGTTATTAAACATGAAGT+TGG - chr8.2:78778681-78778700 MS.gene36754:CDS 25.0%
! CTGACCTTAATTCAATTTAA+AGG - chr8.2:78779460-78779479 MS.gene36754:CDS 25.0%
! TAAATTGAATTAAGGTCAGA+TGG + chr8.2:78779459-78779478 None:intergenic 25.0%
! TAGTTTCATGTGTATTTATC+CGG - chr8.2:78779238-78779257 MS.gene36754:intron 25.0%
! TCATCAATCTTAATTATCAG+TGG - chr8.2:78778388-78778407 MS.gene36754:intron 25.0%
! TCGTTATTAAACATGAAGTT+GGG - chr8.2:78778682-78778701 MS.gene36754:CDS 25.0%
! TCTCTTAATTCAAAACAACA+AGG - chr8.2:78779183-78779202 MS.gene36754:intron 25.0%
!! AATTTTGGATCTTAACTTTC+AGG - chr8.2:78778608-78778627 MS.gene36754:CDS 25.0%
!! ATTTTGGATCTTAACTTTCA+GGG - chr8.2:78778609-78778628 MS.gene36754:CDS 25.0%
!! CAATTTGAAATCCATTCTTT+GGG - chr8.2:78778531-78778550 MS.gene36754:CDS 25.0%
!!! AAACATCACTGAATTTGTTT+TGG + chr8.2:78778755-78778774 None:intergenic 25.0%
!!! ATTTGTTTTGGATAATGCTA+AGG + chr8.2:78778743-78778762 None:intergenic 25.0%
!!! TTCTGATGATTTTTGTTGTT+TGG + chr8.2:78777770-78777789 None:intergenic 25.0%
AAAAATCATCAGAAGAAGAC+CGG - chr8.2:78777776-78777795 MS.gene36754:CDS 30.0%
AATGCTAAGGAACTTAAGAT+CGG + chr8.2:78778730-78778749 None:intergenic 30.0%
ACAATAGAGAAACGAGATTA+GGG + chr8.2:78778203-78778222 None:intergenic 30.0%
AGATGGGATATAAGACATTT+AGG + chr8.2:78779442-78779461 None:intergenic 30.0%
ATAAGACATTTAGGAACACT+TGG + chr8.2:78779433-78779452 None:intergenic 30.0%
ATTAAGAGAACTTGAAGCAT+AGG + chr8.2:78779172-78779191 None:intergenic 30.0%
CATGTTATATATCGACACAT+TGG + chr8.2:78778368-78778387 None:intergenic 30.0%
GAATATTGTACTTCACCATT+AGG + chr8.2:78779582-78779601 None:intergenic 30.0%
TAACCATGTTACATATTCAG+TGG + chr8.2:78779327-78779346 None:intergenic 30.0%
TACAATAGAGAAACGAGATT+AGG + chr8.2:78778204-78778223 None:intergenic 30.0%
TCATAAAAACTGGTTAAGTC+CGG + chr8.2:78779260-78779279 None:intergenic 30.0%
! CTGCCCAAAAATTGAAATTT+TGG - chr8.2:78778593-78778612 MS.gene36754:CDS 30.0%
! GATTATTGCTGATATTTCTG+TGG - chr8.2:78779555-78779574 MS.gene36754:intron 30.0%
! GCAATTTGAAATCCATTCTT+TGG - chr8.2:78778530-78778549 MS.gene36754:CDS 30.0%
! TTTTGTAAAAGGTGATCACA+GGG + chr8.2:78779070-78779089 None:intergenic 30.0%
! TTTTTGTAAAAGGTGATCAC+AGG + chr8.2:78779071-78779090 None:intergenic 30.0%
AATCCAATTGTCAACCGAAT+CGG + chr8.2:78778094-78778113 None:intergenic 35.0%
AATTGGATTCTTGACGTCAT+CGG - chr8.2:78778105-78778124 MS.gene36754:intron 35.0%
ACACCACTGAATATGTAACA+TGG - chr8.2:78779321-78779340 MS.gene36754:intron 35.0%
ACTGAAAAGCTTCAATGCTT+TGG - chr8.2:78777975-78777994 MS.gene36754:CDS 35.0%
ATGCTAAGGAACTTAAGATC+GGG + chr8.2:78778729-78778748 None:intergenic 35.0%
ATTTCTTGGATTACCAGATG+AGG - chr8.2:78779483-78779502 MS.gene36754:CDS 35.0%
CACATGAAACTAATACAGAC+TGG + chr8.2:78779230-78779249 None:intergenic 35.0%
CGAAAATATAGTGACCACAA+TGG + chr8.2:78778056-78778075 None:intergenic 35.0%
TGTTTGTTCCTCAGTATCTT+TGG - chr8.2:78778447-78778466 MS.gene36754:intron 35.0%
! AACGATTTTCAGCCTTTTCA+AGG + chr8.2:78778667-78778686 None:intergenic 35.0%
! AAGATTATCGAAAGTGCGAA+GGG - chr8.2:78779606-78779625 MS.gene36754:CDS 35.0%
! AATCCATTCTTTGGGTTATC+TGG - chr8.2:78778539-78778558 MS.gene36754:CDS 35.0%
! ACTATATTTTCGACGACAAG+TGG - chr8.2:78778064-78778083 MS.gene36754:intron 35.0%
! GTTGAGCATGTTTTGCAAAA+AGG - chr8.2:78779514-78779533 MS.gene36754:CDS 35.0%
! TGATATTTCTGTGGACCTAA+TGG - chr8.2:78779564-78779583 MS.gene36754:intron 35.0%
! TTTAGAGCTCATTCTTCCAT+GGG - chr8.2:78779108-78779127 MS.gene36754:intron 35.0%
!! AACAGTCACCAATTTTAGGA+TGG - chr8.2:78779398-78779417 MS.gene36754:CDS 35.0%
!! ATTTTCAGCCTTTTCAAGGT+AGG + chr8.2:78778663-78778682 None:intergenic 35.0%
!!! ACAGTCACCAATTTTAGGAT+GGG - chr8.2:78779399-78779418 MS.gene36754:CDS 35.0%
AGAGAATTGGAGTTAACCCA+TGG + chr8.2:78779127-78779146 None:intergenic 40.0%
ATTGGATTCTTGACGTCATC+GGG - chr8.2:78778106-78778125 MS.gene36754:intron 40.0%
CTTCCAGATAACCCAAAGAA+TGG + chr8.2:78778545-78778564 None:intergenic 40.0%
GAATTGCACCAAAGATACTG+AGG + chr8.2:78778458-78778477 None:intergenic 40.0%
GATTCCTTCAACTTCAAGGA+TGG - chr8.2:78777937-78777956 MS.gene36754:CDS 40.0%
GGGACAAAACTTCTATGGTT+GGG - chr8.2:78778882-78778901 MS.gene36754:CDS 40.0%
TCAACAAATGACACCTCATC+TGG + chr8.2:78779499-78779518 None:intergenic 40.0%
TCTTCTGTGAATGCAATCCT+CGG - chr8.2:78778567-78778586 MS.gene36754:CDS 40.0%
TGACGATTCCTTCAACTTCA+AGG - chr8.2:78777933-78777952 MS.gene36754:CDS 40.0%
TGATTAACGTCGACAAACAG+TGG - chr8.2:78779032-78779051 MS.gene36754:intron 40.0%
TGGGACAAAACTTCTATGGT+TGG - chr8.2:78778881-78778900 MS.gene36754:CDS 40.0%
! AAATTGGTGACTGTTCCTGT+AGG + chr8.2:78779393-78779412 None:intergenic 40.0%
! ACACCTTTGGAAGAATATCC+AGG - chr8.2:78777897-78777916 MS.gene36754:CDS 40.0%
! CAGGAACAGTCACCAATTTT+AGG - chr8.2:78779394-78779413 MS.gene36754:CDS 40.0%
! GAAGATTATCGAAAGTGCGA+AGG - chr8.2:78779605-78779624 MS.gene36754:CDS 40.0%
! GTTTAGAGCTCATTCTTCCA+TGG - chr8.2:78779107-78779126 MS.gene36754:intron 40.0%
! TCAAGTCTTTTGTTGCCTAC+AGG - chr8.2:78779375-78779394 MS.gene36754:CDS 40.0%
! TGCAAGTTGGCACATTATTG+AGG + chr8.2:78779632-78779651 None:intergenic 40.0%
! TTCCTCGTTTTCTTTTCTCC+CGG - chr8.2:78778147-78778166 MS.gene36754:intron 40.0%
AAGCAAGCAGAGCAGAGAAT+TGG + chr8.2:78779140-78779159 None:intergenic 45.0%
AATTGCAGCAGCTTCAGTGA+TGG + chr8.2:78778516-78778535 None:intergenic 45.0%
ACCAAAACAGTGGTTACCAC+CGG - chr8.2:78777853-78777872 MS.gene36754:CDS 45.0%
AGAGCCATCCTTGAAGTTGA+AGG + chr8.2:78777944-78777963 None:intergenic 45.0%
CATTTAGGAACACTTGGCTG+TGG + chr8.2:78779427-78779446 None:intergenic 45.0%
CGGATCAGTAGATTACCAGA+CGG - chr8.2:78777796-78777815 MS.gene36754:CDS 45.0%
CGTCTGGTAATCTACTGATC+CGG + chr8.2:78777798-78777817 None:intergenic 45.0%
CTGCTGGGACAAAACTTCTA+TGG - chr8.2:78778877-78778896 MS.gene36754:CDS 45.0%
GAGTTTGACTAATGGGACGA+CGG + chr8.2:78778844-78778863 None:intergenic 45.0%
GCACCTGGATATTCTTCCAA+AGG + chr8.2:78777903-78777922 None:intergenic 45.0%
GGCAAACTTTGTCTGAGCTT+TGG + chr8.2:78778635-78778654 None:intergenic 45.0%
GTCCCATTAGTCAAACTCCT+CGG - chr8.2:78778846-78778865 MS.gene36754:CDS 45.0%
TCAATAAACCCCGCGTCATT+CGG - chr8.2:78778019-78778038 MS.gene36754:intron 45.0%
TGTGGAGCCCATCCTAAAAT+TGG + chr8.2:78779409-78779428 None:intergenic 45.0%
TTAGGGCAACTGAAGAGAGT+CGG + chr8.2:78778186-78778205 None:intergenic 45.0%
TTGGAAGAATATCCAGGTGC+TGG - chr8.2:78777903-78777922 MS.gene36754:CDS 45.0%
! TCCTCGTTTTCTTTTCTCCC+GGG - chr8.2:78778148-78778167 MS.gene36754:intron 45.0%
!! ATGCCGATTCGGTTGACAAT+TGG - chr8.2:78778088-78778107 MS.gene36754:intron 45.0%
!! TCAGCCTTTTCAAGGTAGGT+GGG + chr8.2:78778659-78778678 None:intergenic 45.0%
!! TTCAGCCTTTTCAAGGTAGG+TGG + chr8.2:78778660-78778679 None:intergenic 45.0%
!!! GTGGTAACCACTGTTTTGGT+CGG + chr8.2:78777853-78777872 None:intergenic 45.0%
!!! TGGTAACCACTGTTTTGGTC+GGG + chr8.2:78777852-78777871 None:intergenic 45.0%
!! AAAAATAAATAAATAAATAG+GGG + chr8.2:78778234-78778253 None:intergenic 5.0%
!! CAAAAAATAAATAAATAAAT+AGG + chr8.2:78778236-78778255 None:intergenic 5.0%
AAACGAGGAAGAGTTCCTCC+AGG + chr8.2:78778137-78778156 None:intergenic 50.0%
AAGCATCTTCCGAATGACGC+GGG + chr8.2:78778031-78778050 None:intergenic 50.0%
CATGCACAACTTGCACAACG+TGG - chr8.2:78778779-78778798 MS.gene36754:CDS 50.0%
CGCCGAGGAGTTTGACTAAT+GGG + chr8.2:78778851-78778870 None:intergenic 50.0%
CGGAAGATGCTTCTCCATTG+TGG - chr8.2:78778039-78778058 MS.gene36754:intron 50.0%
CGTCATAGAAATCCAGCACC+TGG + chr8.2:78777918-78777937 None:intergenic 50.0%
GAATGACGCGGGGTTTATTG+AGG + chr8.2:78778020-78778039 None:intergenic 50.0%
GTTGGGTGCTTCAACAACCA+AGG - chr8.2:78778899-78778918 MS.gene36754:CDS 50.0%
TCTGTGAATGCAATCCTCGG+CGG - chr8.2:78778570-78778589 MS.gene36754:CDS 50.0%
! GAAGCATCTTCCGAATGACG+CGG + chr8.2:78778032-78778051 None:intergenic 50.0%
! TTTTCTTTTCTCCCGGGCTC+AGG - chr8.2:78778154-78778173 MS.gene36754:intron 50.0%
!!! TAACCACTGTTTTGGTCGGG+AGG + chr8.2:78777849-78777868 None:intergenic 50.0%
ACGACGGAACCAAACGACGA+GGG + chr8.2:78778828-78778847 None:intergenic 55.0%
AGCATCTTCCGAATGACGCG+GGG + chr8.2:78778030-78778049 None:intergenic 55.0%
AGGAAGAGTTCCTCCAGGTG+GGG + chr8.2:78778132-78778151 None:intergenic 55.0%
CCAACGACGAGATAGAGAGC+CGG + chr8.2:78777875-78777894 None:intergenic 55.0%
GACGCGGGGTTTATTGAGGA+GGG + chr8.2:78778016-78778035 None:intergenic 55.0%
GAGGAAGAGTTCCTCCAGGT+GGG + chr8.2:78778133-78778152 None:intergenic 55.0%
GATCTCCTTCCCTCGTCGTT+TGG - chr8.2:78778816-78778835 MS.gene36754:CDS 55.0%
GCACAACTTGCACAACGTGG+TGG - chr8.2:78778782-78778801 MS.gene36754:CDS 55.0%
GCCCGGGAGAAAAGAAAACG+AGG + chr8.2:78778152-78778171 None:intergenic 55.0%
GCCTCCCACCTACCTTGAAA+AGG - chr8.2:78778652-78778671 MS.gene36754:CDS 55.0%
GCGCCGAGGAGTTTGACTAA+TGG + chr8.2:78778852-78778871 None:intergenic 55.0%
GGCAACTGAAGAGAGTCGGA+TGG + chr8.2:78778182-78778201 None:intergenic 55.0%
GGGGTTTATTGAGGAGGGCT+AGG + chr8.2:78778011-78778030 None:intergenic 55.0%
GTCGACAAACAGTGGCTGCT+AGG - chr8.2:78779040-78779059 MS.gene36754:intron 55.0%
TGACGCGGGGTTTATTGAGG+AGG + chr8.2:78778017-78778036 None:intergenic 55.0%
! CGACAAGTGGCATGCCGATT+CGG - chr8.2:78778077-78778096 MS.gene36754:intron 55.0%
! GCCGGTGGTAACCACTGTTT+TGG + chr8.2:78777857-78777876 None:intergenic 55.0%
!! GCCTTTTCAAGGTAGGTGGG+AGG + chr8.2:78778656-78778675 None:intergenic 55.0%
ACGACGAGATAGAGAGCCGG+TGG + chr8.2:78777872-78777891 None:intergenic 60.0%
ACGCCTCCCGACCAAAACAG+TGG - chr8.2:78777843-78777862 MS.gene36754:CDS 60.0%
CCGGCTCTCTATCTCGTCGT+TGG - chr8.2:78777872-78777891 MS.gene36754:CDS 60.0%
CGAGGAAGAGTTCCTCCAGG+TGG + chr8.2:78778134-78778153 None:intergenic 60.0%
CGGAACCAAACGACGAGGGA+AGG + chr8.2:78778824-78778843 None:intergenic 60.0%
CTCGTCGTTGGCGACACCTT+TGG - chr8.2:78777884-78777903 MS.gene36754:CDS 60.0%
GACGACGGAACCAAACGACG+AGG + chr8.2:78778829-78778848 None:intergenic 60.0%
TGGTTGAGCAGCACACCGTC+TGG + chr8.2:78777814-78777833 None:intergenic 60.0%
!! CAATTTTTGGGCAGCCGCCG+AGG + chr8.2:78778587-78778606 None:intergenic 60.0%
!! TTTTGGTCGGGAGGCGTGAG+AGG + chr8.2:78777840-78777859 None:intergenic 60.0%
AGTCGGATGGAGCCTGAGCC+CGG + chr8.2:78778169-78778188 None:intergenic 65.0%
TCCTCGGCGCTCTCTCTGCT+GGG - chr8.2:78778862-78778881 MS.gene36754:CDS 65.0%
! TCGGGAGGCGTGAGAGGATG+TGG + chr8.2:78777834-78777853 None:intergenic 65.0%
CTCCTCGGCGCTCTCTCTGC+TGG - chr8.2:78778861-78778880 MS.gene36754:CDS 70.0%
GTCGGATGGAGCCTGAGCCC+GGG + chr8.2:78778168-78778187 None:intergenic 70.0%
TGACGTCATCGGGCCCCACC+TGG - chr8.2:78778116-78778135 MS.gene36754:intron 70.0%
!! TCCCAGCAGAGAGAGCGCCG+AGG + chr8.2:78778866-78778885 None:intergenic 70.0%
CGTCATCGGGCCCCACCTGG+AGG - chr8.2:78778119-78778138 MS.gene36754:intron 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 78777751 78779660 78777751 ID=MS.gene36754
chr8.2 mRNA 78777751 78779660 78777751 ID=MS.gene36754.t1;Parent=MS.gene36754
chr8.2 exon 78779594 78779660 78779594 ID=MS.gene36754.t1.exon1;Parent=MS.gene36754.t1
chr8.2 CDS 78779594 78779660 78779594 ID=cds.MS.gene36754.t1;Parent=MS.gene36754.t1
chr8.2 exon 78779329 78779548 78779329 ID=MS.gene36754.t1.exon2;Parent=MS.gene36754.t1
chr8.2 CDS 78779329 78779548 78779329 ID=cds.MS.gene36754.t1;Parent=MS.gene36754.t1
chr8.2 exon 78778491 78778950 78778491 ID=MS.gene36754.t1.exon3;Parent=MS.gene36754.t1
chr8.2 CDS 78778491 78778950 78778491 ID=cds.MS.gene36754.t1;Parent=MS.gene36754.t1
chr8.2 exon 78777751 78778014 78777751 ID=MS.gene36754.t1.exon4;Parent=MS.gene36754.t1
chr8.2 CDS 78777751 78778014 78777751 ID=cds.MS.gene36754.t1;Parent=MS.gene36754.t1
Gene Sequence

>MS.gene36754

ATGGAAGAGTCGAACTCCAAACAACAAAAATCATCAGAAGAAGACCGGATCAGTAGATTACCAGACGGTGTGCTGCTCAACCACATCCTCTCACGCCTCCCGACCAAAACAGTGGTTACCACCGGCTCTCTATCTCGTCGTTGGCGACACCTTTGGAAGAATATCCAGGTGCTGGATTTCTATGACGATTCCTTCAACTTCAAGGATGGCTCTTACATTGATCGACTGAAAAGCTTCAATGCTTTGGTGTACAAAGTCCTAGCCCTCCTCAATAAACCCCGCGTCATTCGGAAGATGCTTCTCCATTGTGGTCACTATATTTTCGACGACAAGTGGCATGCCGATTCGGTTGACAATTGGATTCTTGACGTCATCGGGCCCCACCTGGAGGAACTCTTCCTCGTTTTCTTTTCTCCCGGGCTCAGGCTCCATCCGACTCTCTTCAGTTGCCCTAATCTCGTTTCTCTATTGTATAAACTTCCCCTATTTATTTATTTATTTTTTGACTTTTTAAATGACAAAAGTTAGAAGTTCTTGTATGTTAACACTAATAATACTTTGAAAATATGTAACTGAATCTAATCAATATGTTAATGTCGTGTTGTTGTGTAACACCAATGTGTCGATATATAACATGTCATCAATCTTAATTATCAGTGGTATGTAGTTATGTAATGTGATATATCTAACATTATTTGTTTGTTCCTCAGTATCTTTGGTGCAATTCATGTACACGTGCAAAGCTCTATGACAATCAGTTTACCATCACTGAAGCTGCTGCAATTTGAAATCCATTCTTTGGGTTATCTGGAAGTGTCTTCTGTGAATGCAATCCTCGGCGGCTGCCCAAAAATTGAAATTTTGGATCTTAACTTTCAGGGCCAAAGCTCAGACAAAGTTTGCCTCCCACCTACCTTGAAAAGGCTGAAAATCGTTATTAAACATGAAGTTGGGAGTGTGCTTGAAATAAATGCGCCCGATCTTAAGTTCCTTAGCATTATCCAAAACAAATTCAGTGATGTTTTAAACATGCACAACTTGCACAACGTGGTGGAAGCACATCTTGATCTCCTTCCCTCGTCGTTTGGTTCCGTCGTCCCATTAGTCAAACTCCTCGGCGCTCTCTCTGCTGGGACAAAACTTCTATGGTTGGGTGCTTCAACAACCAAGGTCAAGTTTTATTTTAGTTATTTAATGTTTACTTCATCATTTATATAGAGTACTAATTATCATGATTTACTTATGTATATGGATAGATATGCTTTTGTACTAATTACTACTTGATTAACGTCGACAAACAGTGGCTGCTAGGTCAACCCTGTGATCACCTTTTACAAAAATTTCACTATTTGCATCGTTTAGAGCTCATTCTTCCATGGGTTAACTCCAATTCTCTGCTCTGCTTGCTTCAAAAATGTCCTATGCTTCAAGTTCTCTTAATTCAAAACAACAAGGTTTGTTTAATCGAAGCTTAATCCAGTCTGTATTAGTTTCATGTGTATTTATCCGGACTTAACCAGTTTTTATGAAATAGATTACACAAATTAGAACTTGCATCTTACTGCATAGTACACCACTGAATATGTAACATGGTTAGTTCATTACTATGAATATTGTATCTCATTCAAGTCTTTTGTTGCCTACAGGAACAGTCACCAATTTTAGGATGGGCTCCACAGCCAAGTGTTCCTAAATGTCTTATATCCCATCTGACCTTAATTCAATTTAAAGGATTTCTTGGATTACCAGATGAGGTGTCATTTGTTGAGCATGTTTTGCAAAAAGGACTTGTTTTAAAGACAGTGATTATTGCTGATATTTCTGTGGACCTAATGGTGAAGTACAATATTCTCAGAAGATTATCGAAAGTGCGAAGGGCCTCAATAATGTGCCAACTTGCATTTGACTGA
Protein sequence

>MS.gene36754.t1

MEESNSKQQKSSEEDRISRLPDVVTTGSLSRRWRHLWKNIQVLDFYDDSFNFKDGSYIDRLKSFNALVYKVLALLNKPRVIRKMLLHCGHYIFDDNIFGAIHVHVQSSMTISLPSLKLLQFEIHSLGYLEVSSVNAILGGCPKIEILDLNFQGQSSDKVCLPPTLKRLKIVIKHEVGSVLEINAPDLKFLSIIQNKFSDVLNMHNLHNVVEAHLDLLPSSFGSVVPLVKLLGALSAGTKLLWLGASTTKEQSPILGWAPQPSVPKCLISHLTLIQFKGFLGLPDEVSFVEHVLQKGLVLKTVIIADISVDLMVKYNILRRLSKVRRASIMCQLAFD