AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53525


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53525.t1 MTR_1g045140 96.850 127 4 0 1 127 1 127 3.94e-89 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53525.t1 AT2G05620 75.940 133 23 4 2 127 3 133 8.71e-66 196
MS.gene53525.t1 AT2G05620 75.940 133 23 4 2 127 3 133 8.71e-66 196

Find 29 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACGTTTCAATCAGCTTAG+AGG 0.319386 1.2:-27840087 MS.gene53525:CDS
TCATTAACATTCTTCATCAT+AGG 0.355579 1.2:+27840165 None:intergenic
GCCACTTCAATTTCTGCAAC+AGG 0.355663 1.2:-27840315 MS.gene53525:CDS
ACTGAATTCTGCAAATCAAT+AGG 0.397917 1.2:-27839260 MS.gene53525:CDS
AACAGAGACAAGGGTTGATC+AGG 0.409880 1.2:-27839226 MS.gene53525:CDS
TGTGTTGGTTCATCATTCTA+TGG 0.416628 1.2:-27840291 MS.gene53525:CDS
TTACGAGTAACCACAACAAT+TGG 0.430918 1.2:+27840123 None:intergenic
AGCCATTGCTTTACACTCTC+AGG 0.432190 1.2:-27840061 MS.gene53525:intron
GTTTCATCACAAGTTCGTAT+TGG 0.445319 1.2:-27840216 MS.gene53525:CDS
GTTCATCATTCTATGGAAGC+TGG 0.445752 1.2:-27840284 MS.gene53525:CDS
GTGGTTACTCGTAATATTGT+TGG 0.474864 1.2:-27840114 MS.gene53525:CDS
ATTTCTGCAACAGGTTGTGT+TGG 0.484427 1.2:-27840306 MS.gene53525:CDS
AGCTGGGGAACTTCTATAGT+TGG 0.486890 1.2:-27840267 MS.gene53525:CDS
ATCATAGGTTGCAACCTCAC+TGG 0.491055 1.2:+27840180 None:intergenic
TTCATCATTCTATGGAAGCT+GGG 0.491238 1.2:-27840283 MS.gene53525:CDS
ATCAGGTTGGCTAAGAAGAA+TGG 0.492634 1.2:-27839209 MS.gene53525:CDS
ATGATGAAGAATGTTAATGA+AGG 0.502442 1.2:-27840162 MS.gene53525:CDS
TATATTTGCTCCAATTGTTG+TGG 0.534523 1.2:-27840133 MS.gene53525:CDS
AAGAAGAATGGAGAATGGCT+TGG 0.545873 1.2:-27839197 MS.gene53525:CDS
AAGAATGTTAATGAAGGGAA+AGG 0.566696 1.2:-27840156 MS.gene53525:CDS
GAGACAAGGGTTGATCAGGT+TGG 0.575573 1.2:-27839222 MS.gene53525:CDS
ACCTGTTGCAGAAATTGAAG+TGG 0.588366 1.2:+27840314 None:intergenic
TGGCTAAGAAGAATGGAGAA+TGG 0.601122 1.2:-27839202 MS.gene53525:CDS
TGATGAAGAATGTTAATGAA+GGG 0.601637 1.2:-27840161 MS.gene53525:CDS
CACCTGAGAGTGTAAAGCAA+TGG 0.607087 1.2:+27840059 None:intergenic
GCAGATGCAAAACAGAGACA+AGG 0.627180 1.2:-27839236 MS.gene53525:CDS
TCATCATTCTATGGAAGCTG+GGG 0.629743 1.2:-27840282 MS.gene53525:CDS
GAAGAGTTAGTAAGCCAGTG+AGG 0.661265 1.2:-27840194 MS.gene53525:CDS
CAGATGCAAAACAGAGACAA+GGG 0.674066 1.2:-27839235 MS.gene53525:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTTGAATAAAATATGA+TGG - chr1.2:27840161-27840180 MS.gene53525:CDS 10.0%
!! AAACTAAAGATAAAAAATGA+AGG - chr1.2:27839791-27839810 MS.gene53525:intron 15.0%
!! AGAATTTATCAAATGGAAAA+TGG - chr1.2:27839729-27839748 MS.gene53525:intron 20.0%
!! TTCAAGTAGAATTTATCAAA+TGG - chr1.2:27839722-27839741 MS.gene53525:intron 20.0%
!!! ACTTGAATTATGATTTTATG+TGG - chr1.2:27839640-27839659 MS.gene53525:intron 20.0%
!!! CTTCAATATTTTGAAATGTT+AGG - chr1.2:27840214-27840233 MS.gene53525:CDS 20.0%
! AGCAATTGTTTCAAAATCTT+TGG - chr1.2:27840131-27840150 MS.gene53525:CDS 25.0%
! ATGATGAAGAATGTTAATGA+AGG - chr1.2:27839340-27839359 MS.gene53525:intron 25.0%
! ATTGTTTCAAAATCTTTGGT+TGG - chr1.2:27840135-27840154 MS.gene53525:CDS 25.0%
! CATTTAGTATCCTTTCAAAA+AGG + chr1.2:27840008-27840027 None:intergenic 25.0%
! TCATTAACATTCTTCATCAT+AGG + chr1.2:27839340-27839359 None:intergenic 25.0%
! TGATGAAGAATGTTAATGAA+GGG - chr1.2:27839341-27839360 MS.gene53525:intron 25.0%
!! AGTTGAATTTCCTTTTTGAA+AGG - chr1.2:27839995-27840014 MS.gene53525:intron 25.0%
!! TCAAAAACCTTGATTTTATC+TGG - chr1.2:27839929-27839948 MS.gene53525:intron 25.0%
!!! CTTTTTGAAAGGATACTAAA+TGG - chr1.2:27840006-27840025 MS.gene53525:intron 25.0%
!!! GATATTTTAGTGTTACAACT+AGG - chr1.2:27839592-27839611 MS.gene53525:intron 25.0%
AAGAATGTTAATGAAGGGAA+AGG - chr1.2:27839346-27839365 MS.gene53525:intron 30.0%
ACTGAATTCTGCAAATCAAT+AGG - chr1.2:27840242-27840261 MS.gene53525:CDS 30.0%
CAAAGTTTGGTTAGATTGAA+GGG - chr1.2:27839674-27839693 MS.gene53525:intron 30.0%
GGTTTATCTGTTTCAAAGTT+TGG - chr1.2:27839661-27839680 MS.gene53525:intron 30.0%
TATATTTGCTCCAATTGTTG+TGG - chr1.2:27839369-27839388 MS.gene53525:intron 30.0%
TCAAAGTTTGGTTAGATTGA+AGG - chr1.2:27839673-27839692 MS.gene53525:intron 30.0%
TTATATCGCGTAATCAAATG+CGG - chr1.2:27839863-27839882 MS.gene53525:intron 30.0%
! TCTTTCTTGTTCATGTCTTT+TGG - chr1.2:27839548-27839567 MS.gene53525:intron 30.0%
!! GAATTTTGTTTGTGAGGTAT+TGG - chr1.2:27839570-27839589 MS.gene53525:intron 30.0%
!!! CTTTTGGAATTTTGTTTGTG+AGG - chr1.2:27839564-27839583 MS.gene53525:intron 30.0%
AAACGTTTCAATCAGCTTAG+AGG - chr1.2:27839415-27839434 MS.gene53525:intron 35.0%
ACACAGACCAGATAAAATCA+AGG + chr1.2:27839939-27839958 None:intergenic 35.0%
ATGAGAGATTGAGAATTGTG+TGG - chr1.2:27840107-27840126 MS.gene53525:CDS 35.0%
GTGGTTACTCGTAATATTGT+TGG - chr1.2:27839388-27839407 MS.gene53525:intron 35.0%
GTGTAATAGGACTTCGATTA+TGG + chr1.2:27839898-27839917 None:intergenic 35.0%
GTTTCATCACAAGTTCGTAT+TGG - chr1.2:27839286-27839305 MS.gene53525:intron 35.0%
TTACGAGTAACCACAACAAT+TGG + chr1.2:27839382-27839401 None:intergenic 35.0%
TTCATCATTCTATGGAAGCT+GGG - chr1.2:27839219-27839238 MS.gene53525:CDS 35.0%
! GATTTTATCTGGTCTGTGTT+TGG - chr1.2:27839940-27839959 MS.gene53525:intron 35.0%
!! TGTGTTGGTTCATCATTCTA+TGG - chr1.2:27839211-27839230 MS.gene53525:CDS 35.0%
AAGAAGAATGGAGAATGGCT+TGG - chr1.2:27840305-27840324 MS.gene53525:CDS 40.0%
ACCTGTTGCAGAAATTGAAG+TGG + chr1.2:27839191-27839210 None:intergenic 40.0%
ATTTCTGCAACAGGTTGTGT+TGG - chr1.2:27839196-27839215 MS.gene53525:CDS 40.0%
CAGATGCAAAACAGAGACAA+GGG - chr1.2:27840267-27840286 MS.gene53525:CDS 40.0%
GATGTCTCTACGAGTGTAAT+AGG + chr1.2:27839911-27839930 None:intergenic 40.0%
GTTCATCATTCTATGGAAGC+TGG - chr1.2:27839218-27839237 MS.gene53525:CDS 40.0%
TCAAAGTCGTGTAATAGTCG+TGG - chr1.2:27839825-27839844 MS.gene53525:intron 40.0%
TCATCATTCTATGGAAGCTG+GGG - chr1.2:27839220-27839239 MS.gene53525:CDS 40.0%
TGGCTAAGAAGAATGGAGAA+TGG - chr1.2:27840300-27840319 MS.gene53525:CDS 40.0%
!! ATCAGGTTGGCTAAGAAGAA+TGG - chr1.2:27840293-27840312 MS.gene53525:CDS 40.0%
AGCCATTGCTTTACACTCTC+AGG - chr1.2:27839441-27839460 MS.gene53525:intron 45.0%
AGCTGGGGAACTTCTATAGT+TGG - chr1.2:27839235-27839254 MS.gene53525:CDS 45.0%
ATCATAGGTTGCAACCTCAC+TGG + chr1.2:27839325-27839344 None:intergenic 45.0%
GAAGAGTTAGTAAGCCAGTG+AGG - chr1.2:27839308-27839327 MS.gene53525:intron 45.0%
GCAGATGCAAAACAGAGACA+AGG - chr1.2:27840266-27840285 MS.gene53525:CDS 45.0%
GCCACTTCAATTTCTGCAAC+AGG - chr1.2:27839187-27839206 MS.gene53525:CDS 45.0%
! AACAGAGACAAGGGTTGATC+AGG - chr1.2:27840276-27840295 MS.gene53525:CDS 45.0%
! CACCTGAGAGTGTAAAGCAA+TGG + chr1.2:27839446-27839465 None:intergenic 45.0%
!! AAAAATGTAATTTATATATT+AGG - chr1.2:27839757-27839776 MS.gene53525:intron 5.0%
! GAGACAAGGGTTGATCAGGT+TGG - chr1.2:27840280-27840299 MS.gene53525:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 27839184 27840340 27839184 ID=MS.gene53525
chr1.2 mRNA 27839184 27840340 27839184 ID=MS.gene53525.t1;Parent=MS.gene53525
chr1.2 exon 27840062 27840340 27840062 ID=MS.gene53525.t1.exon1;Parent=MS.gene53525.t1
chr1.2 CDS 27840062 27840340 27840062 ID=cds.MS.gene53525.t1;Parent=MS.gene53525.t1
chr1.2 exon 27839184 27839288 27839184 ID=MS.gene53525.t1.exon2;Parent=MS.gene53525.t1
chr1.2 CDS 27839184 27839288 27839184 ID=cds.MS.gene53525.t1;Parent=MS.gene53525.t1
Gene Sequence

>MS.gene53525

ATGGCCACTTCAATTTCTGCAACAGGTTGTGTTGGTTCATCATTCTATGGAAGCTGGGGAACTTCTATAGTTGGTGAAGATTACAGCATGTTAGCTAAGTCAGTTTCATCACAAGTTCGTATTGGAAGAGTTAGTAAGCCAGTGAGGTTGCAACCTATGATGAAGAATGTTAATGAAGGGAAAGGTATATTTGCTCCAATTGTTGTGGTTACTCGTAATATTGTTGGAAAGAAACGTTTCAATCAGCTTAGAGGCAAAGCCATTGCTTTACACTCTCAGGTGAATTAAATTCTAACTTCTTATTGTTTGTGTTGTTTACTATTCTTTTGCATACTTTTTTTAGAAGTTTTTTGAACAATTTCTTTCTTTCTTGTTCATGTCTTTTGGAATTTTGTTTGTGAGGTATTGGATATTTTAGTGTTACAACTAGGAACTTGTTTTTATTTTATATGATTCACTTGAATTATGATTTTATGTGGTTTATCTGTTTCAAAGTTTGGTTAGATTGAAGGGTTTGATGAAATTTGTTGAAGAGAGATTCAAGTAGAATTTATCAAATGGAAAATGGATTTGAAAAATGTAATTTATATATTAGGAATATTAAGACAAACTAAAGATAAAAAATGAAGGATTGAAGTAAATCAAAGTCGTGTAATAGTCGTGGTCGCGTAATAATTGTTTATATCGCGTAATCAAATGCGGCTAATACTGCCATAATCGAAGTCCTATTACACTCGTAGAGACATCAAAAACCTTGATTTTATCTGGTCTGTGTTTGGTTAAATATTGAGATGTGAAATTTTTATAATGAAGTTGAATTTCCTTTTTGAAAGGATACTAAATGGAAGATAAGATAAGATAAGCATTTTATGTAATTTATATATTGAAAGATCAATTAAAAACTACTAATTATCTAACATGTGATGAGAGATTGAGAATTGTGTGGAAGCAATTGTTTCAAAATCTTTGGTTGGCTCTTTATTTGAATAAAATATGATGGAAGCATGCAGCATAATTTTTCTGTACTTAACTTCAATATTTTGAAATGTTAGGTAATTACTGAATTCTGCAAATCAATAGGAGCAGATGCAAAACAGAGACAAGGGTTGATCAGGTTGGCTAAGAAGAATGGAGAATGGCTTGGTTTTCTTGCATGA

Protein sequence

>MS.gene53525.t1

MATSISATGCVGSSFYGSWGTSIVGEDYSMLAKSVSSQVRIGRVSKPVRLQPMMKNVNEGKGIFAPIVVVTRNIVGKKRFNQLRGKAIALHSQVITEFCKSIGADAKQRQGLIRLAKKNGEWLGFLA