AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63261

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63261.t1 MTR_8g467790 70.718 362 66 6 1 353 174 504 5.33e-169 483
MS.gene63261.t1 MTR_8g070950 63.636 407 135 6 1 403 41 438 1.70e-168 479
MS.gene63261.t1 MTR_8g062010 57.590 415 149 10 1 403 35 434 1.23e-144 418
MS.gene63261.t1 MTR_8g062310 53.827 405 156 7 4 403 54 432 6.16e-141 409
MS.gene63261.t1 MTR_8g062080 54.286 420 158 13 1 404 49 450 2.42e-138 403
MS.gene63261.t1 MTR_8g058965 53.089 437 156 10 2 404 40 461 3.61e-134 392
MS.gene63261.t1 MTR_8g469020 50.459 436 168 10 2 403 34 455 6.21e-131 384
MS.gene63261.t1 MTR_8g062300 49.889 449 162 12 1 404 52 482 2.05e-126 374
MS.gene63261.t1 MTR_4g050250 50.781 384 140 12 1 345 47 420 3.94e-108 325
MS.gene63261.t1 MTR_2g083840 41.038 424 208 16 3 399 49 457 1.83e-72 234
MS.gene63261.t1 MTR_2g086020 75.172 145 36 0 259 403 175 319 8.19e-70 223
MS.gene63261.t1 MTR_2g086020 40.397 151 61 6 1 136 1 137 1.94e-18 85.9
MS.gene63261.t1 MTR_8g058970 37.528 445 220 15 1 399 45 477 4.16e-66 218
MS.gene63261.t1 MTR_8g062270 35.363 427 233 13 1 398 41 453 9.26e-66 216
MS.gene63261.t1 MTR_8g014260 37.028 424 223 16 4 399 44 451 1.54e-64 213
MS.gene63261.t1 MTR_8g468810 37.176 425 229 14 1 399 51 463 2.79e-63 210
MS.gene63261.t1 MTR_8g467800 34.038 426 235 12 2 399 46 453 7.20e-59 198
MS.gene63261.t1 MTR_4g032730 32.952 437 239 11 3 398 43 466 7.31e-58 196
MS.gene63261.t1 MTR_8g445780 37.097 372 189 16 4 352 41 390 2.34e-53 182
MS.gene63261.t1 MTR_8g468840 34.279 423 237 14 2 397 40 448 3.26e-51 178
MS.gene63261.t1 MTR_5g092960 32.867 429 241 13 3 398 47 461 1.17e-50 177
MS.gene63261.t1 MTR_8g468760 33.800 429 209 15 1 398 34 418 1.99e-49 172
MS.gene63261.t1 MTR_4g024710 36.700 297 162 11 117 399 63 347 9.79e-40 145
MS.gene63261.t1 MTR_8g014210 38.843 242 127 8 168 399 1 231 1.53e-39 141
MS.gene63261.t1 MTR_8g468810 36.036 333 177 13 1 309 51 371 4.95e-39 144
MS.gene63261.t1 MTR_8g014160 32.452 416 206 18 2 399 67 425 3.06e-37 140
MS.gene63261.t1 MTR_8g077460 47.973 148 73 4 253 399 231 375 1.72e-34 131
MS.gene63261.t1 MTR_8g077460 35.533 197 99 12 4 182 38 224 2.54e-14 74.3
MS.gene63261.t1 MTR_5g092960 32.738 336 186 11 3 309 47 371 6.58e-31 122
MS.gene63261.t1 MTR_8g468810 36.905 252 135 9 1 235 51 295 8.45e-30 117
MS.gene63261.t1 MTR_8g468810 36.905 252 135 9 1 235 51 295 1.13e-29 117
MS.gene63261.t1 MTR_8g468810 36.905 252 135 9 1 235 51 295 1.78e-29 117
MS.gene63261.t1 MTR_3g099800 63.218 87 31 1 311 396 19 105 9.09e-29 108
MS.gene63261.t1 MTR_4g024745 36.620 284 150 13 115 382 15 284 1.10e-28 114
MS.gene63261.t1 MTR_8g467800 32.432 259 143 8 2 239 46 293 3.90e-25 105
MS.gene63261.t1 MTR_7g032370 24.510 408 227 13 2 399 16 352 1.07e-21 95.9
MS.gene63261.t1 MTR_1g041245 25.537 419 222 18 2 399 37 386 1.60e-17 84.0
MS.gene63261.t1 MTR_8g014230 40.909 88 50 2 305 392 13 98 9.34e-17 75.9
MS.gene63261.t1 MTR_5g021550 42.708 96 43 5 4 91 47 138 4.05e-14 70.1
MS.gene63261.t1 MTR_7g098510 35.455 110 71 0 290 399 282 391 4.53e-12 67.4
MS.gene63261.t1 MTR_5g073420 23.206 418 225 18 2 398 37 379 1.93e-11 65.5
MS.gene63261.t1 MTR_5g084070 27.381 252 161 8 150 391 180 419 8.82e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63261.t1 AT2G26030 25.701 428 266 17 2 396 30 438 5.79e-13 70.5
MS.gene63261.t1 AT2G26030 25.701 428 266 17 2 396 30 438 5.79e-13 70.5
MS.gene63261.t1 AT5G44950 25.874 429 261 19 2 396 30 435 7.77e-13 70.1
MS.gene63261.t1 AT2G26030 25.754 431 267 18 2 396 30 443 7.50e-12 67.0
MS.gene63261.t1 AT5G56560 24.146 410 274 15 3 399 33 418 1.91e-11 65.9
MS.gene63261.t1 AT3G42770 23.709 426 256 14 2 391 25 417 5.72e-11 64.7

Find 94 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAAGGTTGAACATTCTT+TGG 0.224083 8.4:-42630384 MS.gene63261:CDS
TGAGTCTCGTCTCTCGAAAA+TGG 0.282794 8.4:-42631086 MS.gene63261:CDS
GGTTGTTGCAGTTGATAAAA+AGG 0.283912 8.4:+42630780 None:intergenic
TGTTGGCATCTTTCATAGTA+TGG 0.287676 8.4:-42630114 MS.gene63261:CDS
CCACCTTTGGGAGCAACTTC+AGG 0.288030 8.4:-42631061 MS.gene63261:CDS
CCAAGTGAAATTGTCATTGA+TGG 0.289134 8.4:+42630187 None:intergenic
CTCGAAAATGGCGCCACCTT+TGG 0.297756 8.4:-42631074 MS.gene63261:CDS
TCGAAAATGGCGCCACCTTT+GGG 0.331452 8.4:-42631073 MS.gene63261:CDS
CTCGGATGCTGTTGAGAATA+GGG 0.353987 8.4:+42630042 None:intergenic
GACCCAGCTGGATCATCTTA+AGG 0.354122 8.4:-42629324 None:intergenic
TGGACTTACTTTGAGTTTGT+TGG 0.358033 8.4:-42630167 MS.gene63261:CDS
TCTCTCTGGCTGCCCCGTGC+TGG 0.363123 8.4:-42630297 MS.gene63261:CDS
TCGAAAGTTGCCTTTCTCTC+TGG 0.376627 8.4:-42630311 MS.gene63261:CDS
GAGCACACTGTCAACGTAAT+AGG 0.392365 8.4:+42630963 None:intergenic
CACGGGGCAGCCAGAGAGAA+AGG 0.396494 8.4:+42630301 None:intergenic
AACTCTGGATAATTTAGAAC+AGG 0.399784 8.4:+42629848 None:intergenic
TCTCGGATGCTGTTGAGAAT+AGG 0.418151 8.4:+42630041 None:intergenic
ATTGATGGATTTCAACCTCT+TGG 0.423461 8.4:+42630202 None:intergenic
ATATCCTTCTATGTGAATAT+AGG 0.424387 8.4:+42629518 None:intergenic
CGATATAATTTGCGAAACTC+TGG 0.433227 8.4:+42629833 None:intergenic
TTGACCTATATTCACATAGA+AGG 0.438936 8.4:-42629522 MS.gene63261:CDS
GATGTCACAATGGGCATTGT+TGG 0.445070 8.4:-42630131 MS.gene63261:CDS
CTGCCCCGTGCTGGAAACTT+TGG 0.445641 8.4:-42630288 MS.gene63261:CDS
ACGGAACTTTCGAATGTTGC+GGG 0.447410 8.4:+42630927 None:intergenic
CAAACTCGACATATGTGAAC+TGG 0.451905 8.4:-42630336 MS.gene63261:CDS
GTGTGGATCCGCGCTGCCAC+TGG 0.452333 8.4:-42630868 MS.gene63261:CDS
GAAACTTTGGATACTATCCT+TGG 0.454621 8.4:-42630275 MS.gene63261:CDS
GTCAACAAACCTTGCTGCGT+TGG 0.455858 8.4:+42629726 None:intergenic
CAACTTAAATTTGACCCAGC+TGG 0.469108 8.4:-42629336 MS.gene63261:CDS
CAGAAGATTTGAGTTAAAGC+AGG 0.472414 8.4:+42629793 None:intergenic
GAGATAGAGTTGTTGAAGGT+GGG 0.478321 8.4:+42630843 None:intergenic
CCATCAATGACAATTTCACT+TGG 0.483121 8.4:-42630187 MS.gene63261:CDS
AGTTTGAGATGCTTCAATGA+TGG 0.488671 8.4:+42630353 None:intergenic
TTATGAGAACTTGAAGCATA+TGG 0.489867 8.4:+42629750 None:intergenic
TAACCTTAAGATGATCCAGC+TGG 0.490301 8.4:+42629321 None:intergenic
GGATTTCAACCTCTTGGAGC+TGG 0.501553 8.4:+42630208 None:intergenic
CAGCTGGGTCAAATTTAAGT+TGG 0.504406 8.4:+42629337 None:intergenic
AACCTCTTGGAGCTGGTGAA+AGG 0.510550 8.4:+42630215 None:intergenic
TCCGGCTGCCATGTTCTCAA+TGG 0.513864 8.4:+42629579 None:intergenic
GTTACTAAACAATCAGCGTC+TGG 0.515478 8.4:+42630814 None:intergenic
ATTCACATAGAAGGATATCA+AGG 0.519668 8.4:-42629513 MS.gene63261:CDS
ATGTATTCCGATGTCACAAT+GGG 0.521692 8.4:-42630140 MS.gene63261:CDS
GTATCCAAAGTTTCCAGCAC+GGG 0.524915 8.4:+42630284 None:intergenic
AACAATGCCCATTGTGACAT+CGG 0.525809 8.4:+42630133 None:intergenic
CCTTGAGATAGAGTTGTTGA+AGG 0.528422 8.4:+42630839 None:intergenic
TCTAGGTGCAATCGAAATGA+AGG 0.529369 8.4:-42630399 MS.gene63261:intron
AAGATTAACAAATGTATCGA+AGG 0.530416 8.4:-42629373 MS.gene63261:CDS
TTCGAATGTTGCGGGATCTG+CGG 0.532075 8.4:+42630935 None:intergenic
TTGTTAATCTTCTGATAGAA+CGG 0.532814 8.4:+42629385 None:intergenic
ACTTACTTTGAGTTTGTTGG+TGG 0.543367 8.4:-42630164 MS.gene63261:CDS
TTTGCGTCATTCAACATCAA+AGG 0.544190 8.4:-42629982 MS.gene63261:intron
GACGGAACTTTCGAATGTTG+CGG 0.546251 8.4:+42630926 None:intergenic
TGAGATAGAGTTGTTGAAGG+TGG 0.549898 8.4:+42630842 None:intergenic
AACAACCACCATTGAGAACA+TGG 0.552420 8.4:-42629587 MS.gene63261:CDS
GGTCTTCAACCTCGATGACT+CGG 0.553196 8.4:-42631040 MS.gene63261:CDS
AGATAGAGTTGTTGAAGGTG+GGG 0.554762 8.4:+42630844 None:intergenic
GTTGAATGACGCAAAAGTAA+TGG 0.560252 8.4:+42629990 None:intergenic
ACCATTGAGAACATGGCAGC+CGG 0.564288 8.4:-42629580 MS.gene63261:CDS
CCGCGTATCCACAGCTGAGA+CGG 0.564606 8.4:+42630908 None:intergenic
TTGTTGAAGGTGGGGTCCAG+TGG 0.565930 8.4:+42630852 None:intergenic
CTCTTGGAGCTGGTGAAAGG+TGG 0.565958 8.4:+42630218 None:intergenic
AAGGTATGTCTCTGTATCCA+AGG 0.569147 8.4:+42630258 None:intergenic
AACCTTAAGATGATCCAGCT+GGG 0.574081 8.4:+42629322 None:intergenic
GGCTGCCATGTTCTCAATGG+TGG 0.577603 8.4:+42629582 None:intergenic
GAAAGGTGGAACAACAAGCT+TGG 0.579670 8.4:+42630232 None:intergenic
TGTCATCTCGAATGTAATCT+CGG 0.579797 8.4:+42630024 None:intergenic
AGAAGATTTGAGTTAAAGCA+GGG 0.583843 8.4:+42629794 None:intergenic
CACCTTTCACCAGCTCCAAG+AGG 0.588939 8.4:-42630217 MS.gene63261:CDS
TCAGAAACTCTTGTTGACTC+CGG 0.591556 8.4:+42629561 None:intergenic
CCTGAAGTTGCTCCCAAAGG+TGG 0.593150 8.4:+42631061 None:intergenic
CAACCTCGATGACTCGGACC+CGG 0.595502 8.4:-42631034 MS.gene63261:CDS
CTATGTGAATATAGGTCAAG+TGG 0.596217 8.4:+42629526 None:intergenic
TATGTATTCCGATGTCACAA+TGG 0.598832 8.4:-42630141 MS.gene63261:CDS
CGAAAGTTCCGTCTCAGCTG+TGG 0.601146 8.4:-42630916 MS.gene63261:CDS
TTTAGAACAGGGGTATGAAG+TGG 0.615375 8.4:+42629860 None:intergenic
CCGTCTCAGCTGTGGATACG+CGG 0.621941 8.4:-42630908 MS.gene63261:CDS
TCTCATAATCCAACGCAGCA+AGG 0.622625 8.4:-42629735 MS.gene63261:intron
AGATTAACAAATGTATCGAA+GGG 0.622978 8.4:-42629372 MS.gene63261:CDS
GGAACAACAAGCTTGGTCAA+AGG 0.624928 8.4:+42630239 None:intergenic
AGACCTGAAGTTGCTCCCAA+AGG 0.633050 8.4:+42631058 None:intergenic
TGGCATCTTTCATAGTATGG+TGG 0.633161 8.4:-42630111 MS.gene63261:CDS
CCTTCAACAACTCTATCTCA+AGG 0.634083 8.4:-42630839 MS.gene63261:CDS
AGTATCCAAAGTTTCCAGCA+CGG 0.634951 8.4:+42630283 None:intergenic
ATGAGAACAAGTTTAAAGTG+TGG 0.635470 8.4:-42630885 MS.gene63261:CDS
TTTGTAATCACAGCCAGTGG+GGG 0.637232 8.4:+42631002 None:intergenic
ACTCTGGATAATTTAGAACA+GGG 0.645860 8.4:+42629849 None:intergenic
TATGTGAATATAGGTCAAGT+GGG 0.652099 8.4:+42629527 None:intergenic
CTCTGGATAATTTAGAACAG+GGG 0.658606 8.4:+42629850 None:intergenic
AGATTACATTCGAGATGACA+CGG 0.661631 8.4:-42630021 MS.gene63261:CDS
TATCCAAAGTTTCCAGCACG+GGG 0.677397 8.4:+42630285 None:intergenic
GGTGGGGTCCAGTGGCAGCG+CGG 0.687223 8.4:+42630860 None:intergenic
TAACTTACGTGAGGGAAACG+AGG 0.688969 8.4:+42630759 None:intergenic
GATTACATTCGAGATGACAC+GGG 0.690284 8.4:-42630020 MS.gene63261:CDS
AGACCGGGTCCGAGTCATCG+AGG 0.724271 8.4:+42631031 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTATAATTTTATTACAA+AGG + chr8.4:42629704-42629723 None:intergenic 10.0%
!!! AGTTATAATTTTATTACAAA+GGG + chr8.4:42629703-42629722 None:intergenic 10.0%
!! AAGTTTATTTAATCAATCTT+TGG - chr8.4:42630715-42630734 MS.gene63261:intron 15.0%
!!! TTACTTGTTAATTAGAAATT+TGG + chr8.4:42629938-42629957 None:intergenic 15.0%
!! ACAAATATATTATTGAGTCA+TGG - chr8.4:42630775-42630794 MS.gene63261:CDS 20.0%
!!! AACATATGTATCTAAGTTTT+AGG + chr8.4:42629845-42629864 None:intergenic 20.0%
!!! AAGGTGATGTTTTATTTTTA+TGG - chr8.4:42630450-42630469 MS.gene63261:intron 20.0%
!!! ACATATGTATCTAAGTTTTA+GGG + chr8.4:42629844-42629863 None:intergenic 20.0%
!!! CATTTGTTGAATATATTTTG+CGG - chr8.4:42630940-42630959 MS.gene63261:CDS 20.0%
!!! TGTTTTTGTTGATATTTCAA+TGG - chr8.4:42630989-42631008 MS.gene63261:CDS 20.0%
! AAGATTAACAAATGTATCGA+AGG - chr8.4:42631040-42631059 MS.gene63261:CDS 25.0%
! AGATTAACAAATGTATCGAA+GGG - chr8.4:42631041-42631060 MS.gene63261:CDS 25.0%
! ATAAGCTTGTAAGAAAGTAA+CGG - chr8.4:42629738-42629757 MS.gene63261:CDS 25.0%
! ATATCCTTCTATGTGAATAT+AGG + chr8.4:42630898-42630917 None:intergenic 25.0%
! GACTCAATAATATATTTGTG+CGG + chr8.4:42630774-42630793 None:intergenic 25.0%
! TTGACTACAAAAGATCTATT+GGG + chr8.4:42629767-42629786 None:intergenic 25.0%
! TTGTTAATCTTCTGATAGAA+CGG + chr8.4:42631031-42631050 None:intergenic 25.0%
! TTTGACTACAAAAGATCTAT+TGG + chr8.4:42629768-42629787 None:intergenic 25.0%
AATGAAGGTTGAACATTCTT+TGG - chr8.4:42630029-42630048 MS.gene63261:CDS 30.0%
ATTCACATAGAAGGATATCA+AGG - chr8.4:42630900-42630919 MS.gene63261:CDS 30.0%
TATATTATTGAGTCATGGCA+TGG - chr8.4:42630780-42630799 MS.gene63261:CDS 30.0%
TATGTGAATATAGGTCAAGT+GGG + chr8.4:42630889-42630908 None:intergenic 30.0%
TTATGAGAACTTGAAGCATA+TGG + chr8.4:42630666-42630685 None:intergenic 30.0%
TTGACCTATATTCACATAGA+AGG - chr8.4:42630891-42630910 MS.gene63261:CDS 30.0%
! AACTCTGGATAATTTAGAAC+AGG + chr8.4:42630568-42630587 None:intergenic 30.0%
! ACTCTGGATAATTTAGAACA+GGG + chr8.4:42630567-42630586 None:intergenic 30.0%
! AGAAGATTTGAGTTAAAGCA+GGG + chr8.4:42630622-42630641 None:intergenic 30.0%
! ATGAGAACAAGTTTAAAGTG+TGG - chr8.4:42629528-42629547 MS.gene63261:CDS 30.0%
! GTTGAATATATTTTGCGGAA+CGG - chr8.4:42630945-42630964 MS.gene63261:CDS 30.0%
!! CAGTTGATAAAAAGGAAGAA+GGG + chr8.4:42629628-42629647 None:intergenic 30.0%
!!! TCTAAGTTTTAGGGTTTGTT+TGG + chr8.4:42629835-42629854 None:intergenic 30.0%
AGATTACATTCGAGATGACA+CGG - chr8.4:42630392-42630411 MS.gene63261:CDS 35.0%
ATGTATTCCGATGTCACAAT+GGG - chr8.4:42630273-42630292 MS.gene63261:CDS 35.0%
CCAAGTGAAATTGTCATTGA+TGG + chr8.4:42630229-42630248 None:intergenic 35.0%
CCATCAATGACAATTTCACT+TGG - chr8.4:42630226-42630245 MS.gene63261:CDS 35.0%
CTATGTGAATATAGGTCAAG+TGG + chr8.4:42630890-42630909 None:intergenic 35.0%
GAAACTTTGGATACTATCCT+TGG - chr8.4:42630138-42630157 MS.gene63261:CDS 35.0%
GTTGAATGACGCAAAAGTAA+TGG + chr8.4:42630426-42630445 None:intergenic 35.0%
TACACGAAAACAACAAGTGA+TGG + chr8.4:42630523-42630542 None:intergenic 35.0%
TATGTATTCCGATGTCACAA+TGG - chr8.4:42630272-42630291 MS.gene63261:CDS 35.0%
TGTCATCTCGAATGTAATCT+CGG + chr8.4:42630392-42630411 None:intergenic 35.0%
TGTTGGCATCTTTCATAGTA+TGG - chr8.4:42630299-42630318 MS.gene63261:CDS 35.0%
TTTGCGTCATTCAACATCAA+AGG - chr8.4:42630431-42630450 MS.gene63261:intron 35.0%
! ACTGCAGTGTTTTCAAAAGA+CGG + chr8.4:42629992-42630011 None:intergenic 35.0%
! AGTTTGAGATGCTTCAATGA+TGG + chr8.4:42630063-42630082 None:intergenic 35.0%
! CAGAAGATTTGAGTTAAAGC+AGG + chr8.4:42630623-42630642 None:intergenic 35.0%
! CGATATAATTTGCGAAACTC+TGG + chr8.4:42630583-42630602 None:intergenic 35.0%
! CTCTGGATAATTTAGAACAG+GGG + chr8.4:42630566-42630585 None:intergenic 35.0%
! TACACCACTTGTTACTACAA+TGG - chr8.4:42629675-42629694 MS.gene63261:intron 35.0%
! TGGACTTACTTTGAGTTTGT+TGG - chr8.4:42630246-42630265 MS.gene63261:CDS 35.0%
! TTTTTGTAATCACAGCCAGT+GGG + chr8.4:42629416-42629435 None:intergenic 35.0%
!! ACTTACTTTGAGTTTGTTGG+TGG - chr8.4:42630249-42630268 MS.gene63261:CDS 35.0%
!! ATTGATGGATTTCAACCTCT+TGG + chr8.4:42630214-42630233 None:intergenic 35.0%
!! GCAGTTGATAAAAAGGAAGA+AGG + chr8.4:42629629-42629648 None:intergenic 35.0%
!! GGTTGTTGCAGTTGATAAAA+AGG + chr8.4:42629636-42629655 None:intergenic 35.0%
AACAACCACCATTGAGAACA+TGG - chr8.4:42630826-42630845 MS.gene63261:CDS 40.0%
AACAATGCCCATTGTGACAT+CGG + chr8.4:42630283-42630302 None:intergenic 40.0%
AAGGTATGTCTCTGTATCCA+AGG + chr8.4:42630158-42630177 None:intergenic 40.0%
AAGTGGTGTAACTTACGTGA+GGG + chr8.4:42629665-42629684 None:intergenic 40.0%
AGATAGAGTTGTTGAAGGTG+GGG + chr8.4:42629572-42629591 None:intergenic 40.0%
AGTATCCAAAGTTTCCAGCA+CGG + chr8.4:42630133-42630152 None:intergenic 40.0%
CAAACTCGACATATGTGAAC+TGG - chr8.4:42630077-42630096 MS.gene63261:CDS 40.0%
CAACTTAAATTTGACCCAGC+TGG - chr8.4:42631077-42631096 MS.gene63261:CDS 40.0%
CAGCTGGGTCAAATTTAAGT+TGG + chr8.4:42631079-42631098 None:intergenic 40.0%
CCTTCAACAACTCTATCTCA+AGG - chr8.4:42629574-42629593 MS.gene63261:CDS 40.0%
CCTTGAGATAGAGTTGTTGA+AGG + chr8.4:42629577-42629596 None:intergenic 40.0%
GAGATAGAGTTGTTGAAGGT+GGG + chr8.4:42629573-42629592 None:intergenic 40.0%
GATTACATTCGAGATGACAC+GGG - chr8.4:42630393-42630412 MS.gene63261:CDS 40.0%
GTTACTAAACAATCAGCGTC+TGG + chr8.4:42629602-42629621 None:intergenic 40.0%
TCTAGGTGCAATCGAAATGA+AGG - chr8.4:42630014-42630033 MS.gene63261:CDS 40.0%
TGAAAACACTGCAGTCTTCT+AGG - chr8.4:42629997-42630016 MS.gene63261:CDS 40.0%
TGAGATAGAGTTGTTGAAGG+TGG + chr8.4:42629574-42629593 None:intergenic 40.0%
TGGCATCTTTCATAGTATGG+TGG - chr8.4:42630302-42630321 MS.gene63261:CDS 40.0%
! CTTTTTGTAATCACAGCCAG+TGG + chr8.4:42629417-42629436 None:intergenic 40.0%
! TGTTTTCAAAAGACGGACAC+AGG + chr8.4:42629985-42630004 None:intergenic 40.0%
! TTGTTTTCGTGTACAACCAG+TGG - chr8.4:42630530-42630549 MS.gene63261:intron 40.0%
! TTTAGAACAGGGGTATGAAG+TGG + chr8.4:42630556-42630575 None:intergenic 40.0%
! TTTTGTAATCACAGCCAGTG+GGG + chr8.4:42629415-42629434 None:intergenic 40.0%
!! TCAGAAACTCTTGTTGACTC+CGG + chr8.4:42630855-42630874 None:intergenic 40.0%
ACGGAACTTTCGAATGTTGC+GGG + chr8.4:42629489-42629508 None:intergenic 45.0%
CAAGTGGTGTAACTTACGTG+AGG + chr8.4:42629666-42629685 None:intergenic 45.0%
CTCGGATGCTGTTGAGAATA+GGG + chr8.4:42630374-42630393 None:intergenic 45.0%
GAAAGGTGGAACAACAAGCT+TGG + chr8.4:42630184-42630203 None:intergenic 45.0%
GACGGAACTTTCGAATGTTG+CGG + chr8.4:42629490-42629509 None:intergenic 45.0%
GAGCACACTGTCAACGTAAT+AGG + chr8.4:42629453-42629472 None:intergenic 45.0%
GGAACAACAAGCTTGGTCAA+AGG + chr8.4:42630177-42630196 None:intergenic 45.0%
GGAGCCATTGTAGTAACAAG+TGG + chr8.4:42629682-42629701 None:intergenic 45.0%
GTATCCAAAGTTTCCAGCAC+GGG + chr8.4:42630132-42630151 None:intergenic 45.0%
TAACTTACGTGAGGGAAACG+AGG + chr8.4:42629657-42629676 None:intergenic 45.0%
TATCCAAAGTTTCCAGCACG+GGG + chr8.4:42630131-42630150 None:intergenic 45.0%
TCGAAAGTTGCCTTTCTCTC+TGG - chr8.4:42630102-42630121 MS.gene63261:CDS 45.0%
TCTCATAATCCAACGCAGCA+AGG - chr8.4:42630678-42630697 MS.gene63261:intron 45.0%
TCTCGGATGCTGTTGAGAAT+AGG + chr8.4:42630375-42630394 None:intergenic 45.0%
TGAGTCTCGTCTCTCGAAAA+TGG - chr8.4:42629327-42629346 MS.gene63261:CDS 45.0%
TTTGTAATCACAGCCAGTGG+GGG + chr8.4:42629414-42629433 None:intergenic 45.0%
! GATGTCACAATGGGCATTGT+TGG - chr8.4:42630282-42630301 MS.gene63261:CDS 45.0%
AACCTCTTGGAGCTGGTGAA+AGG + chr8.4:42630201-42630220 None:intergenic 50.0%
ACCATTGAGAACATGGCAGC+CGG - chr8.4:42630833-42630852 MS.gene63261:CDS 50.0%
AGACCTGAAGTTGCTCCCAA+AGG + chr8.4:42629358-42629377 None:intergenic 50.0%
GGATTTCAACCTCTTGGAGC+TGG + chr8.4:42630208-42630227 None:intergenic 50.0%
GGTCTTCAACCTCGATGACT+CGG - chr8.4:42629373-42629392 MS.gene63261:CDS 50.0%
GTCAACAAACCTTGCTGCGT+TGG + chr8.4:42630690-42630709 None:intergenic 50.0%
GTTCCTCCTGCCAGCAATAA+GGG + chr8.4:42630809-42630828 None:intergenic 50.0%
TCGAAAATGGCGCCACCTTT+GGG - chr8.4:42629340-42629359 MS.gene63261:CDS 50.0%
TGTTCCTCCTGCCAGCAATA+AGG + chr8.4:42630810-42630829 None:intergenic 50.0%
TTCGAATGTTGCGGGATCTG+CGG + chr8.4:42629481-42629500 None:intergenic 50.0%
CACCTTTCACCAGCTCCAAG+AGG - chr8.4:42630196-42630215 MS.gene63261:CDS 55.0%
CCTGAAGTTGCTCCCAAAGG+TGG + chr8.4:42629355-42629374 None:intergenic 55.0%
CGAAAGTTCCGTCTCAGCTG+TGG - chr8.4:42629497-42629516 MS.gene63261:CDS 55.0%
CTCGAAAATGGCGCCACCTT+TGG - chr8.4:42629339-42629358 MS.gene63261:CDS 55.0%
CTCTTGGAGCTGGTGAAAGG+TGG + chr8.4:42630198-42630217 None:intergenic 55.0%
GGCATGGTGTCCCTTATTGC+TGG - chr8.4:42630796-42630815 MS.gene63261:CDS 55.0%
GGCTGCCATGTTCTCAATGG+TGG + chr8.4:42630834-42630853 None:intergenic 55.0%
TCCGGCTGCCATGTTCTCAA+TGG + chr8.4:42630837-42630856 None:intergenic 55.0%
TGGTGTCCCTTATTGCTGGC+AGG - chr8.4:42630800-42630819 MS.gene63261:CDS 55.0%
TGTCCCTTATTGCTGGCAGG+AGG - chr8.4:42630803-42630822 MS.gene63261:CDS 55.0%
TTGTTGAAGGTGGGGTCCAG+TGG + chr8.4:42629564-42629583 None:intergenic 55.0%
! CCACCTTTGGGAGCAACTTC+AGG - chr8.4:42629352-42629371 MS.gene63261:CDS 55.0%
CCGCGTATCCACAGCTGAGA+CGG + chr8.4:42629508-42629527 None:intergenic 60.0%
CCGTCTCAGCTGTGGATACG+CGG - chr8.4:42629505-42629524 MS.gene63261:CDS 60.0%
CTGCCCCGTGCTGGAAACTT+TGG - chr8.4:42630125-42630144 MS.gene63261:CDS 60.0%
! CAACCTCGATGACTCGGACC+CGG - chr8.4:42629379-42629398 MS.gene63261:CDS 60.0%
! CAGGGGTATGAAGTGGCCAC+TGG + chr8.4:42630549-42630568 None:intergenic 60.0%
AGACCGGGTCCGAGTCATCG+AGG + chr8.4:42629385-42629404 None:intergenic 65.0%
CACGGGGCAGCCAGAGAGAA+AGG + chr8.4:42630115-42630134 None:intergenic 65.0%
!! CAGTGGGGGTGTCGAAGACC+GGG + chr8.4:42629400-42629419 None:intergenic 65.0%
!! CCAGTGGGGGTGTCGAAGAC+CGG + chr8.4:42629401-42629420 None:intergenic 65.0%
CCGGTCTTCGACACCCCCAC+TGG - chr8.4:42629398-42629417 MS.gene63261:CDS 70.0%
GTGTGGATCCGCGCTGCCAC+TGG - chr8.4:42629545-42629564 MS.gene63261:CDS 70.0%
TCTCTCTGGCTGCCCCGTGC+TGG - chr8.4:42630116-42630135 MS.gene63261:CDS 70.0%
GGTGGGGTCCAGTGGCAGCG+CGG + chr8.4:42629556-42629575 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 42629326 42631109 42629326 ID=MS.gene63261
chr8.4 mRNA 42629326 42631109 42629326 ID=MS.gene63261.t1;Parent=MS.gene63261
chr8.4 exon 42630767 42631109 42630767 ID=MS.gene63261.t1.exon1;Parent=MS.gene63261.t1
chr8.4 CDS 42630767 42631109 42630767 ID=cds.MS.gene63261.t1;Parent=MS.gene63261.t1
chr8.4 exon 42629983 42630416 42629983 ID=MS.gene63261.t1.exon2;Parent=MS.gene63261.t1
chr8.4 CDS 42629983 42630416 42629983 ID=cds.MS.gene63261.t1;Parent=MS.gene63261.t1
chr8.4 exon 42629736 42629885 42629736 ID=MS.gene63261.t1.exon3;Parent=MS.gene63261.t1
chr8.4 CDS 42629736 42629885 42629736 ID=cds.MS.gene63261.t1;Parent=MS.gene63261.t1
chr8.4 exon 42629326 42629613 42629326 ID=MS.gene63261.t1.exon4;Parent=MS.gene63261.t1
chr8.4 CDS 42629326 42629613 42629326 ID=cds.MS.gene63261.t1;Parent=MS.gene63261.t1
Gene Sequence

>MS.gene63261

ATGAGTCTCGTCTCTCGAAAATGGCGCCACCTTTGGGAGCAACTTCAGGTCTTCAACCTCGATGACTCGGACCCGGTCTTCGACACCCCCACTGGCTGTGATTACAAAAAGTTCAAAAAGTTTGCCTATTACGTTGACAGTGTGCTCGCTCTCCGCAGATCCCGCAACATTCGAAAGTTCCGTCTCAGCTGTGGATACGCGGATGAGAACAAGTTTAAAGTGTGGATCCGCGCTGCCACTGGACCCCACCTTCAACAACTCTATCTCAAGGTTCCAGACGCTGATTGTTTAGTAACACTCCCTTCTTCCTTTTTATCAACTGCAACAACCTCGTTTCCCTCACGTAAGTTACACCACTTGTTACTACAATGGCTCCCTTTGTAATAAAATTATAACTTACAAGCTATAAACTATAAGCTTGTAAGAAAGTAACGGTTACCCAATAGATCTTTTGTAGTCAAATGAGCTTACAAGACATAAGCTATAAGCTATAAAATTATGTCTTACCAAACAAACCCTAAAACTTAGATACATATGTTTTTATTTACTCACTTTTCTTTTTTTTTTTCGATTTTGTTTGCTAGAATACTCATGTTTTACATATTATGTCCAAATTTCTAATTAACAAGTAAACAAATATACATTGTTATCTGTAACCTGTGTCCGTCTTTTGAAAACACTGCAGTCTTCTAGGTGCAATCGAAATGAAGGTTGAACATTCTTTGGTTCATTTTCCATCATTGAAGCATCTCAAACTCGACATATGTGAACTGGATTCGAAAGTTGCCTTTCTCTCTGGCTGCCCCGTGCTGGAAACTTTGGATACTATCCTTGGATACAGAGACATACCTTTGACCAAGCTTGTTGTTCCACCTTTCACCAGCTCCAAGAGGTTGAAATCCATCAATGACAATTTCACTTGGACTTACTTTGAGTTTGTTGGTGGTATGTATTCCGATGTCACAATGGGCATTGTTGGCATCTTTCATAGTATGGTGGAAGCATTTCTTGATGTCTTTTTCTCGAGTGAGAATGAATTTGTCGACCCTATTCTCAACAGCATCCGAGATTACATTCGAGATGACACGGGTGAAGTTCCATTACTTTTGCGTCATTCAACATCAAAGGTGATGTTTTATTTTTATGGTTTATATGTTTTTATATCTGTAAATTGTAATTCGTACTAATATTGCACCATCACTTGTTGTTTTCGTGTACAACCAGTGGCCACTTCATACCCCTGTTCTAAATTATCCAGAGTTTCGCAAATTATATCGTCTAAAGTTCATTCTTCCCTGCTTTAACTCAAATCTTCTGCTCAACGTGCTTGAGAAATGCCATATGCTTCAAGTTCTCATAATCCAACGCAGCAAGGTTTGTTGACTTCTCAAGTTTATTTAATCAATCTTTGGTCTGTCTATATATATGCACGCATATGTATCTATCCGCACAAATATATTATTGAGTCATGGCATGGTGTCCCTTATTGCTGGCAGGAGGAACAACCACCATTGAGAACATGGCAGCCGGAGTCAACAAGAGTTTCTGAATGTCTCAAGTCCCACTTGACCTATATTCACATAGAAGGATATCAAGGACTTGAAGATGAGCTGACATTTGTTGAATATATTTTGCGGAACGGACTTGTTTTGAAGACAATGCTTGTTTTTGTTGATATTTCAATGGACATAACGAACAAGTACCGTTCTATCAGAAGATTAACAAATGTATCGAAGGGCTCTGTCAATTGCCAACTTAAATTTGACCCAGCTGGATCATCTTAA
Protein sequence

>MS.gene63261.t1

MSLVSRKWRHLWEQLQVFNLDDSDPVFDTPTGCDYKKFKKFAYYVDSVLALRRSRNIRKFRLSCGYADENKFKVWIRAATGPHLQQLYLKVPDADCLVTLPSSFLSTATTSFPSRAIEMKVEHSLVHFPSLKHLKLDICELDSKVAFLSGCPVLETLDTILGYRDIPLTKLVVPPFTSSKRLKSINDNFTWTYFEFVGGMYSDVTMGIVGIFHSMVEAFLDVFFSSENEFVDPILNSIRDYIRDDTGEVPLLLRHSTSKWPLHTPVLNYPEFRKLYRLKFILPCFNSNLLLNVLEKCHMLQVLIIQRSKEEQPPLRTWQPESTRVSECLKSHLTYIHIEGYQGLEDELTFVEYILRNGLVLKTMLVFVDISMDITNKYRSIRRLTNVSKGSVNCQLKFDPAGSS