AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79058


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79058.t1 MTR_3g028150 92.958 213 13 1 30 242 32 242 1.18e-137 386
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79058.t1 AT3G63540 78.261 138 30 0 104 241 3 140 4.29e-82 242

Find 46 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATATCTAACTTTCCTTTC+TGG 0.121727 3.1:-20332809 MS.gene79058:CDS
GGTGGAGGAGGAGGAGAAAA+TGG 0.215436 3.1:+20333189 None:intergenic
AAACGTTCCTTCCAACCTTC+TGG 0.272048 3.1:+20332905 None:intergenic
AATATCTAACTTTCCTTTCT+GGG 0.289515 3.1:-20332808 MS.gene79058:CDS
TTCTGCTGAGTCTTTCTTGT+TGG 0.307382 3.1:+20332943 None:intergenic
TTAATATCAAAAGTAGAAAA+AGG 0.345146 3.1:-20332884 MS.gene79058:CDS
GGTGGTTGTTGTGGTGGTGC+TGG 0.347336 3.1:+20333168 None:intergenic
TGTGGTGGTGCTGGTGGAGG+AGG 0.352502 3.1:+20333177 None:intergenic
TGTTGTGGTGGTGCTGGTGG+AGG 0.368074 3.1:+20333174 None:intergenic
GGTGGTGCTGGTGGAGGAGG+AGG 0.373159 3.1:+20333180 None:intergenic
GGTGGTTGTGGTGGTTGTTG+TGG 0.401207 3.1:+20333159 None:intergenic
GGTTGTTGTGGTGGTGCTGG+TGG 0.406983 3.1:+20333171 None:intergenic
GTTAAGGTGGTGGTTATTAG+AGG 0.416358 3.1:+20333097 None:intergenic
TTGTTCAAGACAGCGTCTTT+TGG 0.421438 3.1:+20332770 None:intergenic
TATTATGTTTATGAGATTGA+TGG 0.453881 3.1:-20332656 MS.gene79058:CDS
GTGGAGGAGGAGGAGAAAAT+GGG 0.454320 3.1:+20333190 None:intergenic
GGTTGTGGTGGTTGTTGTGG+TGG 0.455647 3.1:+20333162 None:intergenic
TGTGCCGTAGTAGAGATTGT+AGG 0.481893 3.1:+20333006 None:intergenic
GGGAGATGATTCTGCTGCTA+TGG 0.482398 3.1:+20333027 None:intergenic
GAAGAAGTGAAAGATGATAA+AGG 0.482410 3.1:-20332686 MS.gene79058:CDS
TCTTTATTCCACTCAGGAGT+AGG 0.485770 3.1:+20332563 None:intergenic
GCAGCAAACTACGGCGGCTA+CGG 0.501712 3.1:-20332974 MS.gene79058:CDS
AGGTGGTGATGTTGTGAGAA+TGG 0.508602 3.1:+20333051 None:intergenic
TGATATGGTGGTGGTTGTGG+TGG 0.513282 3.1:+20333150 None:intergenic
AATTTGCAAGATATTATTAG+TGG 0.515902 3.1:-20332728 MS.gene79058:CDS
TCCTTATCTTTATTCCACTC+AGG 0.519893 3.1:+20332557 None:intergenic
TTGTGTTTGAATGAGATAAG+AGG 0.520933 3.1:+20333124 None:intergenic
TGCTGCTGCTGTTAAGGTGG+TGG 0.525546 3.1:+20333087 None:intergenic
TCCTGAGTGGAATAAAGATA+AGG 0.526998 3.1:-20332558 MS.gene79058:CDS
GTGCCGTAGTAGAGATTGTA+GGG 0.548540 3.1:+20333007 None:intergenic
TGCTGCTGCTGCTGTTAAGG+TGG 0.548707 3.1:+20333084 None:intergenic
GAGGAGGAGGAGAAAATGGG+TGG 0.548714 3.1:+20333193 None:intergenic
GCAGCAAGTGCAGCAAACTA+CGG 0.563093 3.1:-20332983 MS.gene79058:CDS
TCTACGACGTTCCAGAAGGT+TGG 0.565732 3.1:-20332916 MS.gene79058:CDS
GAGGAGGAGAAAATGGGTGG+TGG 0.570174 3.1:+20333196 None:intergenic
GCAAACTACGGCGGCTACGG+CGG 0.573351 3.1:-20332971 MS.gene79058:CDS
GATGATTCTGCTGCTATGGA+AGG 0.583786 3.1:+20333031 None:intergenic
CGACGTTCCAGAAGGTTGGA+AGG 0.585452 3.1:-20332912 MS.gene79058:CDS
TCTCCCTACAATCTCTACTA+CGG 0.599583 3.1:-20333010 MS.gene79058:CDS
GCAAGTGCAGCAAACTACGG+CGG 0.619443 3.1:-20332980 MS.gene79058:CDS
TACATCTACGACGTTCCAGA+AGG 0.628307 3.1:-20332920 MS.gene79058:CDS
CTAACTGACGAAACCCAGAA+AGG 0.634618 3.1:+20332795 None:intergenic
TTAATGCTCCTACTCCTGAG+TGG 0.644053 3.1:-20332571 MS.gene79058:CDS
GATTCTGCTGCTATGGAAGG+TGG 0.667537 3.1:+20333034 None:intergenic
TGGTGATGTTGTGAGAATGG+TGG 0.683595 3.1:+20333054 None:intergenic
TGGTGATGATGAGAAAATTG+TGG 0.687506 3.1:+20333216 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATATCAAAAGTAGAAAA+AGG - chr3.1:20332850-20332869 MS.gene79058:CDS 15.0%
!! AATTTGCAAGATATTATTAG+TGG - chr3.1:20333006-20333025 MS.gene79058:CDS 20.0%
!! TATTATGTTTATGAGATTGA+TGG - chr3.1:20333078-20333097 MS.gene79058:CDS 20.0%
! AATATCTAACTTTCCTTTCT+GGG - chr3.1:20332926-20332945 MS.gene79058:CDS 25.0%
!! AAAGTAGAAAAAGGTACAAA+TGG - chr3.1:20332859-20332878 MS.gene79058:CDS 25.0%
GAAGAAGTGAAAGATGATAA+AGG - chr3.1:20333048-20333067 MS.gene79058:CDS 30.0%
GAATATCTAACTTTCCTTTC+TGG - chr3.1:20332925-20332944 MS.gene79058:CDS 30.0%
TTGTGTTTGAATGAGATAAG+AGG + chr3.1:20332613-20332632 None:intergenic 30.0%
! TCTTTCTCTGTTCTTTTCTT+TGG + chr3.1:20332907-20332926 None:intergenic 30.0%
!! TGAGATAAGAGGTTTTGATA+TGG + chr3.1:20332602-20332621 None:intergenic 30.0%
TCCTGAGTGGAATAAAGATA+AGG - chr3.1:20333176-20333195 MS.gene79058:CDS 35.0%
TCCTTATCTTTATTCCACTC+AGG + chr3.1:20333180-20333199 None:intergenic 35.0%
! TGGTGATGATGAGAAAATTG+TGG + chr3.1:20332521-20332540 None:intergenic 35.0%
!! GATAAGAGGTTTTGATATGG+TGG + chr3.1:20332599-20332618 None:intergenic 35.0%
TCTCCCTACAATCTCTACTA+CGG - chr3.1:20332724-20332743 MS.gene79058:CDS 40.0%
TCTTTATTCCACTCAGGAGT+AGG + chr3.1:20333174-20333193 None:intergenic 40.0%
TTCTGCTGAGTCTTTCTTGT+TGG + chr3.1:20332794-20332813 None:intergenic 40.0%
! GTTAAGGTGGTGGTTATTAG+AGG + chr3.1:20332640-20332659 None:intergenic 40.0%
! TTGTTCAAGACAGCGTCTTT+TGG + chr3.1:20332967-20332986 None:intergenic 40.0%
!! AAGAGGTTTTGATATGGTGG+TGG + chr3.1:20332596-20332615 None:intergenic 40.0%
!!! TTTTGATATGGTGGTGGTTG+TGG + chr3.1:20332590-20332609 None:intergenic 40.0%
AAACGTTCCTTCCAACCTTC+TGG + chr3.1:20332832-20332851 None:intergenic 45.0%
CTAACTGACGAAACCCAGAA+AGG + chr3.1:20332942-20332961 None:intergenic 45.0%
GATGATTCTGCTGCTATGGA+AGG + chr3.1:20332706-20332725 None:intergenic 45.0%
GTGCCGTAGTAGAGATTGTA+GGG + chr3.1:20332730-20332749 None:intergenic 45.0%
TACATCTACGACGTTCCAGA+AGG - chr3.1:20332814-20332833 MS.gene79058:CDS 45.0%
TGTGCCGTAGTAGAGATTGT+AGG + chr3.1:20332731-20332750 None:intergenic 45.0%
TTAATGCTCCTACTCCTGAG+TGG - chr3.1:20333163-20333182 MS.gene79058:CDS 45.0%
! AGGTGGTGATGTTGTGAGAA+TGG + chr3.1:20332686-20332705 None:intergenic 45.0%
! TGGTGATGTTGTGAGAATGG+TGG + chr3.1:20332683-20332702 None:intergenic 45.0%
GATTCTGCTGCTATGGAAGG+TGG + chr3.1:20332703-20332722 None:intergenic 50.0%
GCAGCAAGTGCAGCAAACTA+CGG - chr3.1:20332751-20332770 MS.gene79058:CDS 50.0%
GGGAGATGATTCTGCTGCTA+TGG + chr3.1:20332710-20332729 None:intergenic 50.0%
GTGGAGGAGGAGGAGAAAAT+GGG + chr3.1:20332547-20332566 None:intergenic 50.0%
TCTACGACGTTCCAGAAGGT+TGG - chr3.1:20332818-20332837 MS.gene79058:CDS 50.0%
!! TGATATGGTGGTGGTTGTGG+TGG + chr3.1:20332587-20332606 None:intergenic 50.0%
AGCTGCTGCTGCTGCTGTTA+AGG + chr3.1:20332656-20332675 None:intergenic 55.0%
CGACGTTCCAGAAGGTTGGA+AGG - chr3.1:20332822-20332841 MS.gene79058:CDS 55.0%
GAGGAGGAGAAAATGGGTGG+TGG + chr3.1:20332541-20332560 None:intergenic 55.0%
GAGGAGGAGGAGAAAATGGG+TGG + chr3.1:20332544-20332563 None:intergenic 55.0%
GCAAGTGCAGCAAACTACGG+CGG - chr3.1:20332754-20332773 MS.gene79058:CDS 55.0%
GGTGGAGGAGGAGGAGAAAA+TGG + chr3.1:20332548-20332567 None:intergenic 55.0%
GGTGGTTGTGGTGGTTGTTG+TGG + chr3.1:20332578-20332597 None:intergenic 55.0%
GGTTGTGGTGGTTGTTGTGG+TGG + chr3.1:20332575-20332594 None:intergenic 55.0%
TGCTGCTGCTGCTGTTAAGG+TGG + chr3.1:20332653-20332672 None:intergenic 55.0%
TGCTGCTGCTGTTAAGGTGG+TGG + chr3.1:20332650-20332669 None:intergenic 55.0%
GCAGCAAACTACGGCGGCTA+CGG - chr3.1:20332760-20332779 MS.gene79058:CDS 60.0%
! GGTGGTTGTTGTGGTGGTGC+TGG + chr3.1:20332569-20332588 None:intergenic 60.0%
! GGTTGTTGTGGTGGTGCTGG+TGG + chr3.1:20332566-20332585 None:intergenic 60.0%
!! TGTTGTGGTGGTGCTGGTGG+AGG + chr3.1:20332563-20332582 None:intergenic 60.0%
GCAAACTACGGCGGCTACGG+CGG - chr3.1:20332763-20332782 MS.gene79058:CDS 65.0%
!! TGTGGTGGTGCTGGTGGAGG+AGG + chr3.1:20332560-20332579 None:intergenic 65.0%
!! GGTGGTGCTGGTGGAGGAGG+AGG + chr3.1:20332557-20332576 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 20332514 20333242 20332514 ID=MS.gene79058
chr3.1 mRNA 20332514 20333242 20332514 ID=MS.gene79058.t1;Parent=MS.gene79058
chr3.1 exon 20332514 20333242 20332514 ID=MS.gene79058.t1.exon1;Parent=MS.gene79058.t1
chr3.1 CDS 20332514 20333242 20332514 ID=cds.MS.gene79058.t1;Parent=MS.gene79058.t1
Gene Sequence

>MS.gene79058

ATGGCCACAATTTTCTCATCATCACCACCACCCATTTTCTCCTCCTCCTCCACCAGCACCACCACAACAACCACCACAACCACCACCATATCAAAACCTCTTATCTCATTCAAACACAAACAACCTCTAATAACCACCACCTTAACAGCAGCAGCAGCAGCTATAGCCACCATTCTCACAACATCACCACCTTCCATAGCAGCAGAATCATCTCCCTACAATCTCTACTACGGCACAGCAGCAAGTGCAGCAAACTACGGCGGCTACGGCGGAAACTCCAACAAGAAAGACTCAGCAGAATACATCTACGACGTTCCAGAAGGTTGGAAGGAACGTTTAATATCAAAAGTAGAAAAAGGTACAAATGGAACAGACAGTGAGTTCTACAATCCAAAGAAAAGAACAGAGAAAGAATATCTAACTTTCCTTTCTGGGTTTCGTCAGTTAGCACCAAAAGACGCTGTCTTGAACAACTTAGCACTTTCTGATGTGAATTTGCAAGATATTATTAGTGGTGCTGATAATGTGAGTTCTGAAGAAGTGAAAGATGATAAAGGTCAAGTTTATTATGTTTATGAGATTGATGGTGTTGCTTATCATAGCTTGATTTCTGTTACTTGTGCTAATAATAAGCTTTATGCTCATTTTGTTAATGCTCCTACTCCTGAGTGGAATAAAGATAAGGATCTTCTCACTCATGTTCATAAGTCTTTTAAGACTATTGTATAA

Protein sequence

>MS.gene79058.t1

MATIFSSSPPPIFSSSSTSTTTTTTTTTTISKPLISFKHKQPLITTTLTAAAAAIATILTTSPPSIAAESSPYNLYYGTAASAANYGGYGGNSNKKDSAEYIYDVPEGWKERLISKVEKGTNGTDSEFYNPKKRTEKEYLTFLSGFRQLAPKDAVLNNLALSDVNLQDIISGADNVSSEEVKDDKGQVYYVYEIDGVAYHSLISVTCANNKLYAHFVNAPTPEWNKDKDLLTHVHKSFKTIV