AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93083

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93083.t1 MTR_8g445780 76.349 241 57 0 75 315 150 390 1.65e-127 371
MS.gene93083.t1 MTR_8g014210 73.593 231 61 0 131 361 1 231 1.09e-117 340
MS.gene93083.t1 MTR_4g024710 63.066 287 104 2 77 361 61 347 2.36e-116 341
MS.gene93083.t1 MTR_8g014260 63.415 287 103 2 77 361 165 451 9.04e-114 338
MS.gene93083.t1 MTR_8g014260 43.293 164 73 6 1 163 1 145 9.91e-21 93.2
MS.gene93083.t1 MTR_4g024745 62.132 272 101 2 75 344 13 284 5.62e-104 307
MS.gene93083.t1 MTR_8g077460 43.548 372 180 8 16 361 8 375 8.66e-82 254
MS.gene93083.t1 MTR_2g083840 51.351 296 132 7 75 361 165 457 1.64e-79 251
MS.gene93083.t1 MTR_2g083840 56.140 57 24 1 7 62 10 66 1.36e-11 65.9
MS.gene93083.t1 MTR_8g058970 47.447 333 150 10 53 361 146 477 3.08e-76 243
MS.gene93083.t1 MTR_8g058970 56.452 62 24 2 4 62 3 64 2.49e-11 65.1
MS.gene93083.t1 MTR_8g062270 49.117 283 128 7 89 360 176 453 8.26e-75 238
MS.gene93083.t1 MTR_8g468810 51.007 298 133 8 75 361 168 463 1.19e-73 236
MS.gene93083.t1 MTR_8g468810 32.258 186 98 7 4 176 3 173 3.48e-11 64.7
MS.gene93083.t1 MTR_5g092960 50.725 276 118 6 101 361 190 462 9.49e-71 228
MS.gene93083.t1 MTR_8g467800 43.854 301 149 9 77 361 157 453 2.25e-66 217
MS.gene93083.t1 MTR_8g467800 56.716 67 25 1 4 66 2 68 1.40e-14 75.1
MS.gene93083.t1 MTR_8g468840 45.918 294 147 7 75 359 158 448 8.61e-63 207
MS.gene93083.t1 MTR_8g468840 58.824 51 21 0 17 67 13 63 2.18e-12 68.2
MS.gene93083.t1 MTR_4g032730 44.164 317 140 10 74 361 159 467 1.60e-59 199
MS.gene93083.t1 MTR_8g468760 43.243 296 125 7 75 360 156 418 3.30e-57 192
MS.gene93083.t1 MTR_8g468810 51.942 206 91 5 75 274 168 371 6.67e-47 164
MS.gene93083.t1 MTR_8g468810 41.346 104 51 3 4 98 3 105 3.59e-11 64.3
MS.gene93083.t1 MTR_8g058965 39.414 307 160 11 75 361 156 456 1.13e-44 160
MS.gene93083.t1 MTR_8g058965 62.745 51 18 1 18 67 13 63 2.61e-12 68.2
MS.gene93083.t1 MTR_8g014160 36.655 281 113 5 89 361 202 425 3.47e-44 157
MS.gene93083.t1 MTR_8g014160 63.158 57 20 1 14 69 37 93 1.49e-14 74.7
MS.gene93083.t1 MTR_8g469020 36.364 308 169 11 75 361 150 451 1.13e-42 154
MS.gene93083.t1 MTR_8g469020 58.824 51 20 1 18 67 7 57 7.50e-11 63.5
MS.gene93083.t1 MTR_5g092960 53.005 183 76 3 101 274 190 371 1.66e-42 152
MS.gene93083.t1 MTR_8g062080 38.947 285 145 12 89 361 178 445 4.05e-42 152
MS.gene93083.t1 MTR_8g062080 47.945 73 34 2 3 71 5 77 3.91e-11 64.3
MS.gene93083.t1 MTR_8g062310 37.543 293 159 10 74 361 155 428 7.42e-42 151
MS.gene93083.t1 MTR_8g062010 37.801 291 163 10 78 361 151 430 2.49e-37 139
MS.gene93083.t1 MTR_8g062010 60.784 51 19 1 17 66 8 58 9.22e-13 69.3
MS.gene93083.t1 MTR_8g070950 33.553 304 166 11 74 361 151 434 2.31e-36 137
MS.gene93083.t1 MTR_8g070950 58.333 60 21 2 1 59 1 57 1.76e-12 68.6
MS.gene93083.t1 MTR_8g468810 53.846 143 65 1 75 217 168 309 1.47e-34 129
MS.gene93083.t1 MTR_8g468810 41.346 104 51 3 4 98 3 105 2.17e-11 64.7
MS.gene93083.t1 MTR_8g468810 53.846 143 65 1 75 217 168 309 1.83e-34 129
MS.gene93083.t1 MTR_8g468810 41.346 104 51 3 4 98 3 105 2.15e-11 64.7
MS.gene93083.t1 MTR_8g468810 53.846 143 65 1 75 217 168 309 2.47e-34 129
MS.gene93083.t1 MTR_8g468810 41.346 104 51 3 4 98 3 105 2.64e-11 64.7
MS.gene93083.t1 MTR_8g062300 35.274 292 154 9 93 361 198 477 1.56e-33 130
MS.gene93083.t1 MTR_4g050250 38.911 257 128 10 75 307 168 419 6.07e-32 124
MS.gene93083.t1 MTR_8g014230 61.290 93 35 1 269 361 13 104 8.83e-32 115
MS.gene93083.t1 MTR_2g086020 48.855 131 64 3 234 361 185 315 2.14e-26 107
MS.gene93083.t1 MTR_7g098510 27.427 412 227 16 1 361 1 391 1.27e-24 103
MS.gene93083.t1 MTR_7g032380 26.575 365 228 8 18 361 11 356 2.20e-23 99.8
MS.gene93083.t1 MTR_1089s0010 27.990 393 225 16 12 361 2 379 3.02e-23 99.8
MS.gene93083.t1 MTR_7g098570 27.250 400 236 14 1 357 2 389 1.34e-22 99.0
MS.gene93083.t1 MTR_1g041245 26.329 414 197 15 18 361 11 386 4.38e-22 96.7
MS.gene93083.t1 MTR_8g467800 43.137 153 79 5 77 222 157 308 7.41e-22 95.5
MS.gene93083.t1 MTR_8g467800 56.716 67 25 1 4 66 2 68 5.43e-15 75.5
MS.gene93083.t1 MTR_5g021550 54.762 84 37 1 1 83 2 85 1.02e-21 90.9
MS.gene93083.t1 MTR_7g098480 25.971 412 229 11 1 360 1 388 2.48e-21 95.1
MS.gene93083.t1 MTR_5g084070 31.783 258 161 9 104 353 169 419 3.22e-21 94.7
MS.gene93083.t1 MTR_5g008950 26.410 390 214 13 20 361 1 365 5.52e-21 93.6
MS.gene93083.t1 MTR_4g024770 60.606 66 25 1 1 66 8 72 3.54e-20 84.0
MS.gene93083.t1 MTR_5g073420 26.478 389 234 14 12 361 5 380 6.26e-20 90.5
MS.gene93083.t1 MTR_8g014180 71.930 57 14 1 3 59 4 58 9.48e-20 82.4
MS.gene93083.t1 MTR_3g099830 29.024 379 212 19 18 358 12 371 3.78e-19 88.2
MS.gene93083.t1 MTR_8g467790 32.479 234 117 9 75 303 293 490 1.31e-18 87.4
MS.gene93083.t1 MTR_8g014290 71.154 52 15 0 12 63 4 55 2.10e-18 81.6
MS.gene93083.t1 MTR_7g088260 29.082 392 212 17 17 361 90 462 2.20e-18 86.7
MS.gene93083.t1 MTR_4g024720 58.904 73 26 2 1 71 2 72 7.21e-18 81.6
MS.gene93083.t1 MTR_7g056430 28.611 360 224 13 17 353 8 357 2.02e-17 84.3
MS.gene93083.t1 MTR_7g056420 28.611 360 224 13 17 353 8 357 2.03e-17 84.3
MS.gene93083.t1 MTR_5g080710 31.556 225 140 8 112 329 203 420 7.07e-15 75.9
MS.gene93083.t1 MTR_7g023330 25.707 389 203 14 17 345 19 381 1.26e-14 74.7
MS.gene93083.t1 MTR_7g007370 26.415 371 168 13 12 336 13 324 1.50e-14 74.3
MS.gene93083.t1 MTR_1g038710 28.975 283 167 12 74 329 152 427 1.03e-13 72.4
MS.gene93083.t1 MTR_3g114200 51.515 66 30 1 3 66 2 67 1.36e-13 68.2
MS.gene93083.t1 MTR_3g115430 37.391 115 67 3 249 361 14 125 2.46e-13 66.6
MS.gene93083.t1 MTR_3g108560 27.208 283 145 8 88 360 157 388 2.53e-13 70.9
MS.gene93083.t1 MTR_1g038760 57.143 56 24 0 5 60 2 57 5.62e-13 69.7
MS.gene93083.t1 MTR_1g038760 57.143 56 24 0 5 60 2 57 5.83e-13 69.7
MS.gene93083.t1 MTR_5g039970 65.217 46 16 0 17 62 18 63 2.24e-12 67.8
MS.gene93083.t1 MTR_4g080773 30.392 204 110 6 5 181 6 204 2.74e-12 68.2
MS.gene93083.t1 MTR_5g083890 64.583 48 17 0 17 64 5 52 2.90e-12 68.2
MS.gene93083.t1 MTR_4g080773 30.392 204 110 6 5 181 6 204 3.43e-12 67.8
MS.gene93083.t1 MTR_7g098590 46.479 71 36 1 1 71 1 69 4.62e-12 63.2
MS.gene93083.t1 MTR_3g087260 28.000 325 179 13 85 360 138 456 4.91e-12 67.4
MS.gene93083.t1 MTR_7g056447 32.934 167 101 6 17 175 8 171 6.22e-12 65.9
MS.gene93083.t1 MTR_5g039450 26.216 370 224 15 18 358 6 355 6.65e-12 66.6
MS.gene93083.t1 MTR_7g007340 46.154 78 35 3 8 84 2 73 1.16e-11 65.9
MS.gene93083.t1 MTR_4g080813 62.222 45 17 0 18 62 19 63 1.58e-11 60.8
MS.gene93083.t1 MTR_3g023470 53.704 54 25 0 7 60 4 57 1.62e-11 65.5
MS.gene93083.t1 MTR_2g082860 44.737 76 39 1 5 80 17 89 1.69e-11 61.2
MS.gene93083.t1 MTR_3g113782 45.161 62 34 0 3 64 2 63 1.95e-11 60.1
MS.gene93083.t1 MTR_4g051640 50.877 57 28 0 4 60 16 72 1.98e-11 62.4
MS.gene93083.t1 MTR_4g080787 63.043 46 17 0 17 62 18 63 2.56e-11 65.1
MS.gene93083.t1 MTR_2g007090 36.752 117 63 4 18 125 10 124 3.10e-11 63.9
MS.gene93083.t1 MTR_7g110920 28.788 264 145 12 102 329 41 297 3.41e-11 63.9
MS.gene93083.t1 MTR_4g056040 27.027 296 184 11 74 344 168 456 3.51e-11 64.7
MS.gene93083.t1 MTR_4g051460 57.447 47 20 0 17 63 4 50 5.11e-11 58.5
MS.gene93083.t1 MTR_4g092320 57.447 47 20 0 17 63 4 50 5.11e-11 58.5
MS.gene93083.t1 MTR_4g080590 43.678 87 35 3 17 89 22 108 5.58e-11 63.9
MS.gene93083.t1 MTR_4g080590 43.678 87 35 3 17 89 22 108 7.05e-11 63.5
MS.gene93083.t1 MTR_3g105060 46.154 65 35 0 18 82 14 78 8.70e-11 63.2
MS.gene93083.t1 MTR_5g012840 26.316 342 196 13 41 361 12 318 8.71e-11 62.8
MS.gene93083.t1 MTR_2g007500 53.571 56 26 0 7 62 3 58 8.75e-11 62.8
MS.gene93083.t1 MTR_7g081290 47.692 65 33 1 3 66 2 66 9.19e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93083.t1 AT5G38570 27.877 391 217 16 18 360 2 375 3.78e-25 105
MS.gene93083.t1 AT4G26340 30.137 292 182 10 81 361 139 419 4.83e-23 99.8
MS.gene93083.t1 AT3G50710 30.796 289 166 13 89 358 151 424 2.32e-16 80.5
MS.gene93083.t1 AT3G54910 29.286 280 173 13 91 361 110 373 6.67e-16 78.6
MS.gene93083.t1 AT3G54910 29.286 280 173 13 91 361 110 373 6.67e-16 78.6
MS.gene93083.t1 AT3G54910 29.286 280 173 13 91 361 110 373 6.67e-16 78.6
MS.gene93083.t1 AT1G32375 27.211 294 188 10 81 361 142 422 1.29e-15 78.2
MS.gene93083.t1 AT1G32375 27.211 294 188 10 81 361 142 422 1.29e-15 78.2
MS.gene93083.t1 AT5G56430 25.798 376 222 16 20 360 4 357 1.46e-14 74.3
MS.gene93083.t1 AT1G78750 26.912 353 206 17 46 358 115 455 1.48e-14 75.1
MS.gene93083.t1 AT1G78750 26.912 353 206 17 46 358 115 455 1.48e-14 75.1
MS.gene93083.t1 AT5G56390 27.986 293 186 10 81 360 144 424 2.86e-14 73.9
MS.gene93083.t1 AT4G10400 26.950 282 177 13 88 360 147 408 7.46e-14 72.8
MS.gene93083.t1 AT4G10400 26.950 282 177 13 88 360 147 408 7.46e-14 72.8
MS.gene93083.t1 AT4G10400 26.950 282 177 13 88 360 147 408 7.46e-14 72.8
MS.gene93083.t1 AT5G56452 27.386 241 150 10 129 361 3 226 1.19e-13 70.1
MS.gene93083.t1 AT4G00160 29.276 304 176 15 81 361 155 442 1.42e-13 72.0
MS.gene93083.t1 AT5G56452 27.984 243 147 10 129 361 3 227 2.65e-13 68.9
MS.gene93083.t1 AT5G56420 27.972 286 179 12 89 361 151 422 2.68e-13 71.2
MS.gene93083.t1 AT5G56420 27.972 286 179 12 89 361 151 422 2.68e-13 71.2
MS.gene93083.t1 AT5G56420 27.972 286 179 12 89 361 151 422 2.68e-13 71.2
MS.gene93083.t1 AT5G56820 27.645 293 165 12 89 358 155 423 4.34e-13 70.5
MS.gene93083.t1 AT3G49480 25.589 297 189 10 73 350 152 435 1.32e-12 68.9
MS.gene93083.t1 AT3G49480 25.418 299 191 10 73 352 12 297 1.39e-12 68.2
MS.gene93083.t1 AT4G13965 30.323 310 178 15 72 358 126 420 2.47e-12 68.2
MS.gene93083.t1 AT3G52680 31.741 293 166 15 89 361 167 445 3.18e-12 67.8
MS.gene93083.t1 AT1G78760 26.957 345 207 16 48 358 107 440 1.67e-11 65.9
MS.gene93083.t1 AT3G49030 29.268 287 175 12 89 360 167 440 2.48e-11 65.1
MS.gene93083.t1 AT5G56440 44.706 85 42 3 18 101 2 82 3.49e-11 64.7
MS.gene93083.t1 AT4G26350 26.421 299 179 11 88 361 146 428 4.00e-11 64.3
MS.gene93083.t1 AT1G06630 26.939 245 157 7 87 318 165 400 4.14e-11 64.3
MS.gene93083.t1 AT1G06630 26.939 245 157 7 87 318 165 400 4.14e-11 64.3
MS.gene93083.t1 AT1G06630 26.939 245 157 7 87 318 165 400 4.14e-11 64.3
MS.gene93083.t1 AT1G06630 26.939 245 157 7 87 318 165 400 4.14e-11 64.3
MS.gene93083.t1 AT1G06630 26.939 245 157 7 87 318 174 409 4.25e-11 64.3
MS.gene93083.t1 AT1G16930 24.917 301 182 11 89 358 159 446 4.36e-11 64.3
MS.gene93083.t1 AT1G16930 24.917 301 182 11 89 358 159 446 4.36e-11 64.3
MS.gene93083.t1 AT1G16930 24.917 301 182 11 89 358 159 446 4.36e-11 64.3
MS.gene93083.t1 AT1G06630 27.149 221 146 6 87 301 165 376 7.53e-11 63.5
MS.gene93083.t1 AT4G15060 26.070 257 123 5 18 274 172 361 9.16e-11 63.5

Find 95 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGACCTTTATTCAATTTAA+AGG 0.136086 8.2:+33020577 MS.gene93083:CDS
AACCTTCTTGGCGCTCTCTC+TGG 0.222591 8.2:+33019210 MS.gene93083:CDS
GCCAGTTGTAACGGCGGTTT+TGG 0.282218 8.2:-33017845 None:intergenic
GATTCTCGTACTTCTTCTTT+AGG 0.286538 8.2:-33020696 None:intergenic
TCTTCTTCGATTTCTTCTTC+TGG 0.286797 8.2:-33017766 None:intergenic
CGCCTAGAGCTCAATCTTCC+CGG 0.291407 8.2:+33020203 MS.gene93083:CDS
TCTTTGTTAATCAGTCTCTA+TGG 0.310114 8.2:+33018808 MS.gene93083:intron
ATCCCTTTACACAACCTTCT+TGG 0.322992 8.2:+33019198 MS.gene93083:CDS
AAATCCTTTAAATTGAATAA+AGG 0.339709 8.2:-33020581 None:intergenic
ACCAAAACCGCCGTTACAAC+TGG 0.366112 8.2:+33017844 MS.gene93083:CDS
GATTATTGCTGATTGTTCCT+TGG 0.366763 8.2:+33020672 MS.gene93083:CDS
AACATTACCCGAATCACATT+TGG 0.369562 8.2:+33019096 MS.gene93083:CDS
CGCCAAGAAGGTTGTGTAAA+GGG 0.386248 8.2:-33019200 None:intergenic
TCCAGCCTTCTTACGGAAGA+TGG 0.394716 8.2:-33019009 None:intergenic
GATGTCTTTCCCCAATCGTT+TGG 0.397912 8.2:+33019168 MS.gene93083:CDS
ACCTTCTTGGCGCTCTCTCT+GGG 0.399525 8.2:+33019211 MS.gene93083:CDS
AAGCAAGGTGAGCAGAGAAT+TGG 0.401087 8.2:-33020234 None:intergenic
GATCAGGCGCATTTATTTCA+AGG 0.403961 8.2:-33019062 None:intergenic
AGCTTGGCCAAATGTGATTC+GGG 0.414911 8.2:-33019103 None:intergenic
ATTCAATTTAAAGGATTTCA+AGG 0.418336 8.2:+33020586 MS.gene93083:CDS
AACCCCAACTTCATTCTCAT+TGG 0.421872 8.2:-33019037 None:intergenic
CAAACTTTGTCCAAGCTTTG+CGG 0.422685 8.2:-33018982 None:intergenic
CTGAATCCATCTAACCGTTC+GGG 0.436295 8.2:-33017949 None:intergenic
AAGCTTGGCCAAATGTGATT+CGG 0.437271 8.2:-33019104 None:intergenic
TGGGTAGCCCATCCTAAAAC+TGG 0.440402 8.2:-33020523 None:intergenic
GACAATTCGGAACACTTGGC+TGG 0.444024 8.2:-33020543 None:intergenic
ATGTGATTCGGGTAATGTTA+AGG 0.445469 8.2:-33019092 None:intergenic
AACGTGAATGCACTTCTTTG+TGG 0.447771 8.2:+33018922 MS.gene93083:CDS
GGCGATTTCCAGCCTTCTTA+CGG 0.449202 8.2:-33019016 None:intergenic
AACTTAACCATGGTTGTTGA+AGG 0.457176 8.2:-33019255 None:intergenic
TTTGAAGTGCCAAGGGCCTC+AGG 0.467940 8.2:+33020730 MS.gene93083:CDS
CTCGCCGCTGGCGATACCTC+TGG 0.470009 8.2:+33017875 MS.gene93083:CDS
CCCATCTTCCGTAAGAAGGC+TGG 0.470819 8.2:+33019008 MS.gene93083:CDS
GATGACAGAACCAAACGATT+GGG 0.471945 8.2:-33019178 None:intergenic
TCTCTCTGGGACTAAACATC+TGG 0.472916 8.2:+33019224 MS.gene93083:CDS
ATCGCCAATGAGAATGAAGT+TGG 0.478040 8.2:+33019033 MS.gene93083:CDS
TCGCCAATGAGAATGAAGTT+GGG 0.480417 8.2:+33019034 MS.gene93083:CDS
AGGTGGGATACGAGACAATT+CGG 0.483168 8.2:-33020556 None:intergenic
CTTTAAATTGAATAAAGGTC+AGG 0.486856 8.2:-33020576 None:intergenic
ATTTGCAGTGTCTTTAATGA+TGG 0.487972 8.2:-33018874 None:intergenic
TCAACAAATGACACCTCATC+TGG 0.488517 8.2:-33020613 None:intergenic
GTGTCCCATCTTCCGTAAGA+AGG 0.494377 8.2:+33019004 MS.gene93083:CDS
TAGTTTCTCTCCGCAAAGCT+TGG 0.495255 8.2:+33018972 MS.gene93083:CDS
ATGTTAAGGTACTCAAGATC+AGG 0.497396 8.2:-33019078 None:intergenic
TGTATTCATCGAACCCTTCA+CGG 0.505166 8.2:+33018840 MS.gene93083:CDS
CCAGCCTTCTTACGGAAGAT+GGG 0.510309 8.2:-33019008 None:intergenic
AGATTAAGAGATTCAATGAT+GGG 0.511116 8.2:-33018949 None:intergenic
AAACATTATTTGAAGTGCCA+AGG 0.517129 8.2:+33020722 MS.gene93083:CDS
GCAAATCAGCATGGATTATG+TGG 0.517789 8.2:+33018891 MS.gene93083:CDS
TGACACATTCCTGAGGCCCT+TGG 0.520521 8.2:-33020739 None:intergenic
ATTATTAGAGCTTGAAGCAT+GGG 0.520762 8.2:-33020266 None:intergenic
ACCCGGGAAGATTGAGCTCT+AGG 0.521976 8.2:-33020205 None:intergenic
GTACTTCTTCTTTAGGTCCA+AGG 0.526366 8.2:-33020689 None:intergenic
GGATGACAGAACCAAACGAT+TGG 0.529615 8.2:-33019179 None:intergenic
ATGTGGAGAAGTACACCGTC+TGG 0.535753 8.2:-33017805 None:intergenic
GCCTAGAGCTCAATCTTCCC+GGG 0.536635 8.2:+33020204 MS.gene93083:CDS
AAGATTAAGAGATTCAATGA+TGG 0.540155 8.2:-33018950 None:intergenic
GCGCCAAGAAGGTTGTGTAA+AGG 0.540668 8.2:-33019201 None:intergenic
CAGAGAATTGGAGTTAAACC+CGG 0.543776 8.2:-33020222 None:intergenic
ACGAGACAATTCGGAACACT+TGG 0.544655 8.2:-33020547 None:intergenic
TAAATTGAATAAAGGTCAGG+TGG 0.546131 8.2:-33020573 None:intergenic
CGCCAATGAGAATGAAGTTG+GGG 0.556833 8.2:+33019035 MS.gene93083:CDS
TCCCAGAGAGAGCGCCAAGA+AGG 0.560841 8.2:-33019212 None:intergenic
TTGGGAGACGTGATAGGATG+TGG 0.562368 8.2:-33017822 None:intergenic
AGATAGACGGCCAGTTGTAA+CGG 0.562751 8.2:-33017854 None:intergenic
AACATTATTTGAAGTGCCAA+GGG 0.565951 8.2:+33020723 MS.gene93083:CDS
GGCAAACTAACCACCGTGAA+GGG 0.567050 8.2:-33018853 None:intergenic
TAATCCATCATCAATGGCAT+CGG 0.581132 8.2:+33017723 None:intergenic
ATCGCCAGCGGCGAGATAGA+CGG 0.587569 8.2:-33017867 None:intergenic
AATTATTAGAGCTTGAAGCA+TGG 0.590362 8.2:-33020267 None:intergenic
AGAGAATTGGAGTTAAACCC+GGG 0.591395 8.2:-33020221 None:intergenic
AGTTGTACATGCTGAAAGCT+TGG 0.596389 8.2:-33019119 None:intergenic
GTGTACAAACAGTGGTTGCT+TGG 0.596834 8.2:+33020137 MS.gene93083:intron
CTGGCCGTCTATCTCGCCGC+TGG 0.598014 8.2:+33017863 MS.gene93083:CDS
ATCAAGTCCGTGAGATTCGT+CGG 0.601030 8.2:-33017923 None:intergenic
ACAATTCGGAACACTTGGCT+GGG 0.602510 8.2:-33020542 None:intergenic
TTCGTCGGCGAAGTCGAGAA+CGG 0.610913 8.2:-33017908 None:intergenic
CATGGGACATTTCTGAAGCA+AGG 0.613005 8.2:-33020249 None:intergenic
GTTGAATCCTTCAACAACCA+TGG 0.615902 8.2:+33019248 MS.gene93083:CDS
ATTTCAAGGATTTCCAGATG+AGG 0.618723 8.2:+33020600 MS.gene93083:CDS
TGGCAAACTAACCACCGTGA+AGG 0.622599 8.2:-33018854 None:intergenic
GACTTCGCCGACGAATCTCA+CGG 0.628163 8.2:+33017916 MS.gene93083:CDS
CGAATCAGTAATTTACCAGA+CGG 0.632147 8.2:+33017790 MS.gene93083:CDS
ATGACAGAACCAAACGATTG+GGG 0.634767 8.2:-33019177 None:intergenic
AAATTGAATAAAGGTCAGGT+GGG 0.634827 8.2:-33020572 None:intergenic
AAAGACACTGCAAATCAGCA+TGG 0.640510 8.2:+33018882 MS.gene93083:CDS
TCTAATAATTCAGAATGACA+AGG 0.656418 8.2:+33020280 MS.gene93083:CDS
GTACAACTTACACAATGTCG+TGG 0.661367 8.2:+33019134 MS.gene93083:CDS
GATTAAGAGATTCAATGATG+GGG 0.667084 8.2:-33018948 None:intergenic
GAACGGAGAGATGCTGCCAG+AGG 0.679033 8.2:-33017891 None:intergenic
GCTGCCAGAGGTATCGCCAG+CGG 0.682009 8.2:-33017879 None:intergenic
TTTAAGTTGACACATTCCTG+AGG 0.695966 8.2:-33020746 None:intergenic
TAGACGGCCAGTTGTAACGG+CGG 0.704524 8.2:-33017851 None:intergenic
ACGGACTTGATTCACCCGAA+CGG 0.705095 8.2:+33017935 MS.gene93083:CDS
ATTCATCGAACCCTTCACGG+TGG 0.791743 8.2:+33018843 MS.gene93083:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAAAGAAAATTTAG+AGG + chr8.2:33018716-33018735 MS.gene93083:intron 10.0%
!! TATTAATAAATCATGATTAT+TGG - chr8.2:33020076-33020095 None:intergenic 10.0%
!! AAAGTGAAAGAAAAAAAAAA+GGG - chr8.2:33019434-33019453 None:intergenic 15.0%
!! AAATCCTTTAAATTGAATAA+AGG - chr8.2:33020584-33020603 None:intergenic 15.0%
!! ACATAATAATTAGAAATTGT+TGG + chr8.2:33018510-33018529 MS.gene93083:intron 15.0%
!! TAAAGTGAAAGAAAAAAAAA+AGG - chr8.2:33019435-33019454 None:intergenic 15.0%
!! TGTAATCTATTCATAAAAAT+TGG - chr8.2:33020357-33020376 None:intergenic 15.0%
!!! AATTTTCTCATATATAAATC+TGG + chr8.2:33018264-33018283 MS.gene93083:intron 15.0%
!!! CAACTATATTTTGTTTATAT+GGG - chr8.2:33019704-33019723 None:intergenic 15.0%
!!! TAATCTTGTTATATATTAAG+TGG - chr8.2:33020413-33020432 None:intergenic 15.0%
!!! TCAACTATATTTTGTTTATA+TGG - chr8.2:33019705-33019724 None:intergenic 15.0%
!! AAGTTAAATTGACCAAATTA+AGG - chr8.2:33020320-33020339 None:intergenic 20.0%
!! ATTCAATTTAAAGGATTTCA+AGG + chr8.2:33020586-33020605 MS.gene93083:CDS 20.0%
!! GAAATAAAAAAACTTAACCA+TGG - chr8.2:33019268-33019287 None:intergenic 20.0%
!!! GCAATATTCATTTTTTGAAA+AGG - chr8.2:33020465-33020484 None:intergenic 20.0%
!!! GTTTTTAAAATATGATGATG+TGG - chr8.2:33019363-33019382 None:intergenic 20.0%
!!! TTAAAAAGAACTGATTCTTT+GGG - chr8.2:33019626-33019645 None:intergenic 20.0%
!!! TTTAAAAAGAACTGATTCTT+TGG - chr8.2:33019627-33019646 None:intergenic 20.0%
! AAAACAAAAAGAATTGACAC+AGG + chr8.2:33019454-33019473 MS.gene93083:intron 25.0%
! AAGATTAAGAGATTCAATGA+TGG - chr8.2:33018953-33018972 None:intergenic 25.0%
! AGATTAAGAGATTCAATGAT+GGG - chr8.2:33018952-33018971 None:intergenic 25.0%
! ATTATTGAAATGTTTGCAAC+AGG - chr8.2:33019894-33019913 None:intergenic 25.0%
! ATTCTTGAAAAAGTAGATCA+TGG - chr8.2:33020169-33020188 None:intergenic 25.0%
! CTGACCTTTATTCAATTTAA+AGG + chr8.2:33020577-33020596 MS.gene93083:CDS 25.0%
! CTTTAAATTGAATAAAGGTC+AGG - chr8.2:33020579-33020598 None:intergenic 25.0%
! GATTGAGCTTATGAAAAATA+TGG - chr8.2:33018468-33018487 None:intergenic 25.0%
! TAAATTGACCAAATTAAGGT+TGG - chr8.2:33020316-33020335 None:intergenic 25.0%
! TAATCCAAATTGAAACAAAC+CGG + chr8.2:33019865-33019884 MS.gene93083:intron 25.0%
! TCTAATAATTCAGAATGACA+AGG + chr8.2:33020280-33020299 MS.gene93083:CDS 25.0%
! TGTTTAATCCAACCTTAATT+TGG + chr8.2:33020305-33020324 MS.gene93083:intron 25.0%
! TTCACAAAGAAAAGAGAAAA+CGG + chr8.2:33019587-33019606 MS.gene93083:intron 25.0%
! TTCTTGAAAAAGTAGATCAT+GGG - chr8.2:33020168-33020187 None:intergenic 25.0%
! TTGTACTAATTACTACTTGA+TGG + chr8.2:33020113-33020132 MS.gene93083:intron 25.0%
!! ACAAAAATCGTTAAAAGTGT+TGG - chr8.2:33019770-33019789 None:intergenic 25.0%
!! CTTTTTCCTTCCATAAAATA+CGG - chr8.2:33018354-33018373 None:intergenic 25.0%
!! TAAAAAGAACTGATTCTTTG+GGG - chr8.2:33019625-33019644 None:intergenic 25.0%
!!! AAGTGTTGGAATTTCAAATA+TGG - chr8.2:33019756-33019775 None:intergenic 25.0%
!!! ACTCTATATGCTTGTTATTA+CGG + chr8.2:33018141-33018160 MS.gene93083:intron 25.0%
!!! ATTTTGTTTATATGGGTATG+TGG - chr8.2:33019697-33019716 None:intergenic 25.0%
!!! CATAAGTTATTTTCCAAATG+AGG + chr8.2:33018442-33018461 MS.gene93083:intron 25.0%
AAAACTCATTACCTAAGAAC+CGG + chr8.2:33019378-33019397 MS.gene93083:intron 30.0%
AAATTGAATAAAGGTCAGGT+GGG - chr8.2:33020575-33020594 None:intergenic 30.0%
AATTATTAGAGCTTGAAGCA+TGG - chr8.2:33020270-33020289 None:intergenic 30.0%
ACACTTCTCACATTATAAGT+CGG + chr8.2:33018627-33018646 MS.gene93083:intron 30.0%
ACAGTAAGGAACAAATTTAG+AGG + chr8.2:33018682-33018701 MS.gene93083:intron 30.0%
ATGAAAAATATGGCCTCATT+TGG - chr8.2:33018458-33018477 None:intergenic 30.0%
ATTATTAGAGCTTGAAGCAT+GGG - chr8.2:33020269-33020288 None:intergenic 30.0%
ATTATTATGTAACCCGACTA+TGG - chr8.2:33018500-33018519 None:intergenic 30.0%
ATTTGCAGTGTCTTTAATGA+TGG - chr8.2:33018877-33018896 None:intergenic 30.0%
GATTAAGAGATTCAATGATG+GGG - chr8.2:33018951-33018970 None:intergenic 30.0%
GTTCCTTACTGTTGAAATTT+GGG - chr8.2:33018674-33018693 None:intergenic 30.0%
TAAATTGAATAAAGGTCAGG+TGG - chr8.2:33020576-33020595 None:intergenic 30.0%
TCACAAAGAAAAGAGAAAAC+GGG + chr8.2:33019588-33019607 MS.gene93083:intron 30.0%
TCTTGAAAAAGTAGATCATG+GGG - chr8.2:33020167-33020186 None:intergenic 30.0%
TCTTTGTTAATCAGTCTCTA+TGG + chr8.2:33018808-33018827 MS.gene93083:intron 30.0%
TGTCACATCAGTTTATAAAG+AGG + chr8.2:33018602-33018621 MS.gene93083:intron 30.0%
TGTTCCTTACTGTTGAAATT+TGG - chr8.2:33018675-33018694 None:intergenic 30.0%
TTCATAAGCTCAATCCAAAT+GGG + chr8.2:33018473-33018492 MS.gene93083:intron 30.0%
TTCCTTACTGTTGAAATTTG+GGG - chr8.2:33018673-33018692 None:intergenic 30.0%
TTTCATAAGCTCAATCCAAA+TGG + chr8.2:33018472-33018491 MS.gene93083:intron 30.0%
! AAACATTATTTGAAGTGCCA+AGG + chr8.2:33020722-33020741 MS.gene93083:CDS 30.0%
! AACATTATTTGAAGTGCCAA+GGG + chr8.2:33020723-33020742 MS.gene93083:CDS 30.0%
! CAAGCTTTTACCGTATTTTA+TGG + chr8.2:33018341-33018360 MS.gene93083:intron 30.0%
! CTTTTACCGTATTTTATGGA+AGG + chr8.2:33018345-33018364 MS.gene93083:intron 30.0%
!! TTTTAAAAAAGAGCGATGCT+AGG - chr8.2:33019974-33019993 None:intergenic 30.0%
AACATTACCCGAATCACATT+TGG + chr8.2:33019096-33019115 MS.gene93083:CDS 35.0%
AACTTAACCATGGTTGTTGA+AGG - chr8.2:33019258-33019277 None:intergenic 35.0%
ACATTTCAATAATAGCCACG+TGG + chr8.2:33019901-33019920 MS.gene93083:intron 35.0%
ATATGCACTCCCTCAATAAT+TGG + chr8.2:33019336-33019355 MS.gene93083:intron 35.0%
ATGATGTGGACCAATTATTG+AGG - chr8.2:33019349-33019368 None:intergenic 35.0%
ATTTCAAGGATTTCCAGATG+AGG + chr8.2:33020600-33020619 MS.gene93083:CDS 35.0%
CATTTCAATAATAGCCACGT+GGG + chr8.2:33019902-33019921 MS.gene93083:intron 35.0%
CGAATCAGTAATTTACCAGA+CGG + chr8.2:33017790-33017809 MS.gene93083:CDS 35.0%
GAGAATCAAACACAATCTCA+TGG + chr8.2:33019500-33019519 MS.gene93083:intron 35.0%
GATTCTCGTACTTCTTCTTT+AGG - chr8.2:33020699-33020718 None:intergenic 35.0%
TCATAAGCTCAATCCAAATG+GGG + chr8.2:33018474-33018493 MS.gene93083:intron 35.0%
TCTTCTTCGATTTCTTCTTC+TGG - chr8.2:33017769-33017788 None:intergenic 35.0%
TGAGATTGTGTTTGATTCTC+AGG - chr8.2:33019500-33019519 None:intergenic 35.0%
TGATGTGGACCAATTATTGA+GGG - chr8.2:33019348-33019367 None:intergenic 35.0%
TGCTGCAAATGAGATAACTA+TGG - chr8.2:33019673-33019692 None:intergenic 35.0%
! AACTTTATCGATTGAGTCGT+GGG + chr8.2:33018077-33018096 MS.gene93083:intron 35.0%
! ATGTGATTCGGGTAATGTTA+AGG - chr8.2:33019095-33019114 None:intergenic 35.0%
! ATGTTAAGGTACTCAAGATC+AGG - chr8.2:33019081-33019100 None:intergenic 35.0%
! GATTATTGCTGATTGTTCCT+TGG + chr8.2:33020672-33020691 MS.gene93083:CDS 35.0%
! GTTGAGCATGTTTTGCAAAA+AGG + chr8.2:33020631-33020650 MS.gene93083:CDS 35.0%
! TTTTCGATTATGACCCTGTT+TGG + chr8.2:33018298-33018317 MS.gene93083:intron 35.0%
!! TATGCTTGTTATTACGGTTC+TGG + chr8.2:33018147-33018166 MS.gene93083:intron 35.0%
!! TTTAAGTTGACACATTCCTG+AGG - chr8.2:33020749-33020768 None:intergenic 35.0%
AAAGACACTGCAAATCAGCA+TGG + chr8.2:33018882-33018901 MS.gene93083:CDS 40.0%
AAATGTTTGCAACAGGCAAC+CGG - chr8.2:33019887-33019906 None:intergenic 40.0%
AACCCCAACTTCATTCTCAT+TGG - chr8.2:33019040-33019059 None:intergenic 40.0%
AACGTGAATGCACTTCTTTG+TGG + chr8.2:33018922-33018941 MS.gene93083:CDS 40.0%
AAGCCAAACAAGGAAACTAG+AGG - chr8.2:33018560-33018579 None:intergenic 40.0%
AAGCTTGGCCAAATGTGATT+CGG - chr8.2:33019107-33019126 None:intergenic 40.0%
AGAGAATTGGAGTTAAACCC+GGG - chr8.2:33020224-33020243 None:intergenic 40.0%
AGGCAGATCACAATAAAGGT+GGG - chr8.2:33018540-33018559 None:intergenic 40.0%
AGTTGTACATGCTGAAAGCT+TGG - chr8.2:33019122-33019141 None:intergenic 40.0%
ATCCCTTTACACAACCTTCT+TGG + chr8.2:33019198-33019217 MS.gene93083:CDS 40.0%
ATCGCCAATGAGAATGAAGT+TGG + chr8.2:33019033-33019052 MS.gene93083:CDS 40.0%
ATGACAGAACCAAACGATTG+GGG - chr8.2:33019180-33019199 None:intergenic 40.0%
CAAACTTTGTCCAAGCTTTG+CGG - chr8.2:33018985-33019004 None:intergenic 40.0%
CAGAGAATTGGAGTTAAACC+CGG - chr8.2:33020225-33020244 None:intergenic 40.0%
CTAGAGGCAGATCACAATAA+AGG - chr8.2:33018544-33018563 None:intergenic 40.0%
GACCCCAAATTTCAACAGTA+AGG + chr8.2:33018668-33018687 MS.gene93083:intron 40.0%
GATCAGGCGCATTTATTTCA+AGG - chr8.2:33019065-33019084 None:intergenic 40.0%
GATGACAGAACCAAACGATT+GGG - chr8.2:33019181-33019200 None:intergenic 40.0%
GATTGTGTTTGATTCTCAGG+TGG - chr8.2:33019497-33019516 None:intergenic 40.0%
GCATAAGCTCAATCCAAACA+GGG - chr8.2:33018314-33018333 None:intergenic 40.0%
GTACAACTTACACAATGTCG+TGG + chr8.2:33019134-33019153 MS.gene93083:CDS 40.0%
GTACTTCTTCTTTAGGTCCA+AGG - chr8.2:33020692-33020711 None:intergenic 40.0%
GTTGAATCCTTCAACAACCA+TGG + chr8.2:33019248-33019267 MS.gene93083:CDS 40.0%
TCAACAAATGACACCTCATC+TGG - chr8.2:33020616-33020635 None:intergenic 40.0%
TCCATGTAAACCAGTATAGC+CGG - chr8.2:33019400-33019419 None:intergenic 40.0%
TCGCCAATGAGAATGAAGTT+GGG + chr8.2:33019034-33019053 MS.gene93083:CDS 40.0%
TCTGAATCCATCTAACCGTT+CGG - chr8.2:33017953-33017972 None:intergenic 40.0%
TGCATAAGCTCAATCCAAAC+AGG - chr8.2:33018315-33018334 None:intergenic 40.0%
TGTATTCATCGAACCCTTCA+CGG + chr8.2:33018840-33018859 MS.gene93083:CDS 40.0%
TTGAATACTTGCGCAGTGAT+TGG - chr8.2:33019928-33019947 None:intergenic 40.0%
! GAACTTTATCGATTGAGTCG+TGG + chr8.2:33018076-33018095 MS.gene93083:intron 40.0%
! GCAAATCAGCATGGATTATG+TGG + chr8.2:33018891-33018910 MS.gene93083:CDS 40.0%
!! AAGTGAAGAGTGTTTGAGGT+AGG - chr8.2:33018179-33018198 None:intergenic 40.0%
!! CATATGAGAGAGTGTTAGAG+AGG - chr8.2:33019321-33019340 None:intergenic 40.0%
!! CTTGTTTGGCTTGACTTTGA+GGG + chr8.2:33018568-33018587 MS.gene93083:intron 40.0%
!! GCAACCGGTTTGTTTCAATT+TGG - chr8.2:33019872-33019891 None:intergenic 40.0%
!! TTGTTTGGCTTGACTTTGAG+GGG + chr8.2:33018569-33018588 MS.gene93083:intron 40.0%
!!! AACAGTCACCAGTTTTAGGA+TGG + chr8.2:33020515-33020534 MS.gene93083:CDS 40.0%
!!! ACAGTCACCAGTTTTAGGAT+GGG + chr8.2:33020516-33020535 MS.gene93083:CDS 40.0%
!!! TTCTGGTTTTCGTTGTCGTT+TGG - chr8.2:33017752-33017771 None:intergenic 40.0%
AAGCAAGGTGAGCAGAGAAT+TGG - chr8.2:33020237-33020256 None:intergenic 45.0%
AATCCAAATGGGGCCATAGT+CGG + chr8.2:33018484-33018503 MS.gene93083:intron 45.0%
ACAATTCGGAACACTTGGCT+GGG - chr8.2:33020545-33020564 None:intergenic 45.0%
ACGAGACAATTCGGAACACT+TGG - chr8.2:33020550-33020569 None:intergenic 45.0%
AGATAGACGGCCAGTTGTAA+CGG - chr8.2:33017857-33017876 None:intergenic 45.0%
AGCTTGGCCAAATGTGATTC+GGG - chr8.2:33019106-33019125 None:intergenic 45.0%
AGGTGGGATACGAGACAATT+CGG - chr8.2:33020559-33020578 None:intergenic 45.0%
ATCAAGTCCGTGAGATTCGT+CGG - chr8.2:33017926-33017945 None:intergenic 45.0%
CCTCAAAGTCAAGCCAAACA+AGG - chr8.2:33018570-33018589 None:intergenic 45.0%
CGCCAAGAAGGTTGTGTAAA+GGG - chr8.2:33019203-33019222 None:intergenic 45.0%
CGCCAATGAGAATGAAGTTG+GGG + chr8.2:33019035-33019054 MS.gene93083:CDS 45.0%
CTGAATCCATCTAACCGTTC+GGG - chr8.2:33017952-33017971 None:intergenic 45.0%
GAGGCAGATCACAATAAAGG+TGG - chr8.2:33018541-33018560 None:intergenic 45.0%
GATGTCTTTCCCCAATCGTT+TGG + chr8.2:33019168-33019187 MS.gene93083:CDS 45.0%
GGAAGATGAGTCTCAACTGT+CGG + chr8.2:33018031-33018050 MS.gene93083:intron 45.0%
GGATGACAGAACCAAACGAT+TGG - chr8.2:33019182-33019201 None:intergenic 45.0%
GTGTACAAACAGTGGTTGCT+TGG + chr8.2:33020137-33020156 MS.gene93083:intron 45.0%
TACCTAAGAACCGGCTATAC+TGG + chr8.2:33019387-33019406 MS.gene93083:intron 45.0%
TAGTTTCTCTCCGCAAAGCT+TGG + chr8.2:33018972-33018991 MS.gene93083:CDS 45.0%
TCCATAATCCACGTGGCATT+AGG + chr8.2:33018010-33018029 MS.gene93083:intron 45.0%
TTGATTCTCAGGTGGTGAGT+GGG - chr8.2:33019489-33019508 None:intergenic 45.0%
TTTGATTCTCAGGTGGTGAG+TGG - chr8.2:33019490-33019509 None:intergenic 45.0%
! AACCAGTATAGCCGGTTCTT+AGG - chr8.2:33019392-33019411 None:intergenic 45.0%
! AATGCCACGTGGATTATGGA+GGG - chr8.2:33018010-33018029 None:intergenic 45.0%
! CAGGAACAGTCACCAGTTTT+AGG + chr8.2:33020511-33020530 MS.gene93083:intron 45.0%
! CTGCCTCTAGTTTCCTTGTT+TGG + chr8.2:33018554-33018573 MS.gene93083:intron 45.0%
! TAATGCCACGTGGATTATGG+AGG - chr8.2:33018011-33018030 None:intergenic 45.0%
! TCCTAATGCCACGTGGATTA+TGG - chr8.2:33018014-33018033 None:intergenic 45.0%
!! ACCGGCTATACTGGTTTACA+TGG + chr8.2:33019396-33019415 MS.gene93083:intron 45.0%
!! CATGGGACATTTCTGAAGCA+AGG - chr8.2:33020252-33020271 None:intergenic 45.0%
!! CCTTGTTTGGCTTGACTTTG+AGG + chr8.2:33018567-33018586 MS.gene93083:intron 45.0%
!! GAGGAAGTGAAGAGTGTTTG+AGG - chr8.2:33018183-33018202 None:intergenic 45.0%
!! TCTCTCTGGGACTAAACATC+TGG + chr8.2:33019224-33019243 MS.gene93083:CDS 45.0%
!! TGTTTGGCTTGACTTTGAGG+GGG + chr8.2:33018570-33018589 MS.gene93083:intron 45.0%
!! TTGATGGCGTGTACAAACAG+TGG + chr8.2:33020129-33020148 MS.gene93083:intron 45.0%
!! TTTTGGTTGGGAGACGTGAT+AGG - chr8.2:33017831-33017850 None:intergenic 45.0%
!!! TTGTAACGGCGGTTTTGGTT+GGG - chr8.2:33017843-33017862 None:intergenic 45.0%
AAACTGGTGACTGTTCCTGC+AGG - chr8.2:33020510-33020529 None:intergenic 50.0%
AACTGGTGACTGTTCCTGCA+GGG - chr8.2:33020509-33020528 None:intergenic 50.0%
AAGCTCGAGTTCCTCAAGGT+AGG - chr8.2:33018121-33018140 None:intergenic 50.0%
ACCAAAACCGCCGTTACAAC+TGG + chr8.2:33017844-33017863 MS.gene93083:CDS 50.0%
ACGGACTTGATTCACCCGAA+CGG + chr8.2:33017935-33017954 MS.gene93083:CDS 50.0%
ACTGTTCCTGCAGGGAACAA+AGG - chr8.2:33020501-33020520 None:intergenic 50.0%
AGCTCGAGTTCCTCAAGGTA+GGG - chr8.2:33018120-33018139 None:intergenic 50.0%
ATCCAAATGGGGCCATAGTC+GGG + chr8.2:33018485-33018504 MS.gene93083:intron 50.0%
ATGCATGCCTGAGTGATACG+AGG - chr8.2:33018209-33018228 None:intergenic 50.0%
ATTCATCGAACCCTTCACGG+TGG + chr8.2:33018843-33018862 MS.gene93083:CDS 50.0%
CCAGCCTTCTTACGGAAGAT+GGG - chr8.2:33019011-33019030 None:intergenic 50.0%
CCTCTAACCTCGTATCACTC+AGG + chr8.2:33018199-33018218 MS.gene93083:intron 50.0%
CCTGAGTGATACGAGGTTAG+AGG - chr8.2:33018202-33018221 None:intergenic 50.0%
CTCATCTTCCTAATGCCACG+TGG - chr8.2:33018021-33018040 None:intergenic 50.0%
GACAATTCGGAACACTTGGC+TGG - chr8.2:33020546-33020565 None:intergenic 50.0%
GAGTAAGCTCGAGTTCCTCA+AGG - chr8.2:33018125-33018144 None:intergenic 50.0%
GATTCACCCGAACGGTTAGA+TGG + chr8.2:33017943-33017962 MS.gene93083:intron 50.0%
GCGCCAAGAAGGTTGTGTAA+AGG - chr8.2:33019204-33019223 None:intergenic 50.0%
GGCAAACTAACCACCGTGAA+GGG - chr8.2:33018856-33018875 None:intergenic 50.0%
GGCGATTTCCAGCCTTCTTA+CGG - chr8.2:33019019-33019038 None:intergenic 50.0%
GTGTCCCATCTTCCGTAAGA+AGG + chr8.2:33019004-33019023 MS.gene93083:CDS 50.0%
TACAGCCTTGCCTGTTGACA+AGG + chr8.2:33019797-33019816 MS.gene93083:intron 50.0%
TCAAGTCCTTTGTTCCCTGC+AGG + chr8.2:33020492-33020511 MS.gene93083:intron 50.0%
TCCAGCCTTCTTACGGAAGA+TGG - chr8.2:33019012-33019031 None:intergenic 50.0%
TGGCAAACTAACCACCGTGA+AGG - chr8.2:33018857-33018876 None:intergenic 50.0%
TGGGTAGCCCATCCTAAAAC+TGG - chr8.2:33020526-33020545 None:intergenic 50.0%
TTCCTCAAGGTAGGGTCCAA+TGG - chr8.2:33018112-33018131 None:intergenic 50.0%
TTGGGAGACGTGATAGGATG+TGG - chr8.2:33017825-33017844 None:intergenic 50.0%
! ATGTGGAGAAGTACACCGTC+TGG - chr8.2:33017808-33017827 None:intergenic 50.0%
!! GTTGTAACGGCGGTTTTGGT+TGG - chr8.2:33017844-33017863 None:intergenic 50.0%
AACCCGACTATGGCCCCATT+TGG - chr8.2:33018490-33018509 None:intergenic 55.0%
AACCTTCTTGGCGCTCTCTC+TGG + chr8.2:33019210-33019229 MS.gene93083:CDS 55.0%
ACCCGGGAAGATTGAGCTCT+AGG - chr8.2:33020208-33020227 None:intergenic 55.0%
ACCTTCTTGGCGCTCTCTCT+GGG + chr8.2:33019211-33019230 MS.gene93083:CDS 55.0%
AGGTGGTGAGTGGGAACGAA+GGG - chr8.2:33019480-33019499 None:intergenic 55.0%
CATTGCCTCGCCTTGTCAAC+AGG - chr8.2:33019810-33019829 None:intergenic 55.0%
CCCATCTTCCGTAAGAAGGC+TGG + chr8.2:33019008-33019027 MS.gene93083:CDS 55.0%
CGCCTAGAGCTCAATCTTCC+CGG + chr8.2:33020203-33020222 MS.gene93083:CDS 55.0%
CTCAAGGTAGGGTCCAATGG+CGG - chr8.2:33018109-33018128 None:intergenic 55.0%
CTCTCCCTCCATAATCCACG+TGG + chr8.2:33018003-33018022 MS.gene93083:intron 55.0%
GACTTCGCCGACGAATCTCA+CGG + chr8.2:33017916-33017935 MS.gene93083:CDS 55.0%
GCCTAGAGCTCAATCTTCCC+GGG + chr8.2:33020204-33020223 MS.gene93083:CDS 55.0%
TAGACGGCCAGTTGTAACGG+CGG - chr8.2:33017854-33017873 None:intergenic 55.0%
TGACACATTCCTGAGGCCCT+TGG - chr8.2:33020742-33020761 None:intergenic 55.0%
TTCGTCGGCGAAGTCGAGAA+CGG - chr8.2:33017911-33017930 None:intergenic 55.0%
! CACGTGGATTATGGAGGGAG+AGG - chr8.2:33018005-33018024 None:intergenic 55.0%
! GCCAGTTGTAACGGCGGTTT+TGG - chr8.2:33017848-33017867 None:intergenic 55.0%
! TTGGCTTGACTTTGAGGGGG+TGG + chr8.2:33018573-33018592 MS.gene93083:intron 55.0%
! TTTGAAGTGCCAAGGGCCTC+AGG + chr8.2:33020730-33020749 MS.gene93083:CDS 55.0%
ATCGCCAGCGGCGAGATAGA+CGG - chr8.2:33017870-33017889 None:intergenic 60.0%
CAGGTGGTGAGTGGGAACGA+AGG - chr8.2:33019481-33019500 None:intergenic 60.0%
CCTCGCCTTGTCAACAGGCA+AGG - chr8.2:33019805-33019824 None:intergenic 60.0%
CCTTGCCTGTTGACAAGGCG+AGG + chr8.2:33019802-33019821 MS.gene93083:intron 60.0%
CGCCATTGGACCCTACCTTG+AGG + chr8.2:33018107-33018126 MS.gene93083:intron 60.0%
GAACGGAGAGATGCTGCCAG+AGG - chr8.2:33017894-33017913 None:intergenic 60.0%
!! TCCCAGAGAGAGCGCCAAGA+AGG - chr8.2:33019215-33019234 None:intergenic 60.0%
TCGTGGGTTCGTGCCGCCAT+TGG + chr8.2:33018093-33018112 MS.gene93083:intron 65.0%
! TGCGCAGTGATTGGCCCACG+TGG - chr8.2:33019919-33019938 None:intergenic 65.0%
!! GCTGCCAGAGGTATCGCCAG+CGG - chr8.2:33017882-33017901 None:intergenic 65.0%
CTCGCCGCTGGCGATACCTC+TGG + chr8.2:33017875-33017894 MS.gene93083:CDS 70.0%
CTGGCCGTCTATCTCGCCGC+TGG + chr8.2:33017863-33017882 MS.gene93083:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 33017736 33020777 33017736 ID=MS.gene93083
chr8.2 mRNA 33017736 33020777 33017736 ID=MS.gene93083.t1;Parent=MS.gene93083
chr8.2 exon 33017736 33017956 33017736 ID=MS.gene93083.t1.exon1;Parent=MS.gene93083.t1
chr8.2 CDS 33017736 33017956 33017736 ID=cds.MS.gene93083.t1;Parent=MS.gene93083.t1
chr8.2 exon 33018822 33019269 33018822 ID=MS.gene93083.t1.exon2;Parent=MS.gene93083.t1
chr8.2 CDS 33018822 33019269 33018822 ID=cds.MS.gene93083.t1;Parent=MS.gene93083.t1
chr8.2 exon 33020149 33020301 33020149 ID=MS.gene93083.t1.exon3;Parent=MS.gene93083.t1
chr8.2 CDS 33020149 33020301 33020149 ID=cds.MS.gene93083.t1;Parent=MS.gene93083.t1
chr8.2 exon 33020514 33020777 33020514 ID=MS.gene93083.t1.exon4;Parent=MS.gene93083.t1
chr8.2 CDS 33020514 33020777 33020514 ID=cds.MS.gene93083.t1;Parent=MS.gene93083.t1
Gene Sequence

>MS.gene93083

ATGGCATCGGAATCCAAACGACAACGAAAACCAGAAGAAGAAATCGAAGAAGATCGAATCAGTAATTTACCAGACGGTGTACTTCTCCACATCCTATCACGTCTCCCAACCAAAACCGCCGTTACAACTGGCCGTCTATCTCGCCGCTGGCGATACCTCTGGCAGCATCTCTCCGTTCTCGACTTCGCCGACGAATCTCACGGACTTGATTCACCCGAACGGTTAGATGGATTCAGAAGTTTCTCTGTTTTAGTTAACAGCGTCTTCCTCTCCCTCCATAATCCACGTGGCATTAGGAAGATGAGTCTCAACTGTCGGTCTCTTTTTTATGATAAGTGTCGAACTTTATCGATTGAGTCGTGGGTTCGTGCCGCCATTGGACCCTACCTTGAGGAACTCGAGCTTACTCTATATGCTTGTTATTACGGTTCTGGATTCAACCTACCTCAAACACTCTTCACTTCCTCTAACCTCGTATCACTCAGGCATGCATGCATAACGTTGAACTAATTGTTTTTTCGTCGACTTAATTTTCTCATATATAAATCTGGAGTGAGTGTTTTTTTCGATTATGACCCTGTTTGGATTGAGCTTATGCAGATAAACAAGCTTTTACCGTATTTTATGGAAGGAAAAAGAAAAAAAACTTATGAAAATATTTTCATCAGTGAGAGCTTATGAATTAATATAAAAACTTATTTATTTGCATAAGTTATTTTCCAAATGAGGCCATATTTTTCATAAGCTCAATCCAAATGGGGCCATAGTCGGGTTACATAATAATTAGAAATTGTTGGTAATCCCACCTTTATTGTGATCTGCCTCTAGTTTCCTTGTTTGGCTTGACTTTGAGGGGGTGGATTTAGTGTCACATCAGTTTATAAAGAGGAGACACTTCTCACATTATAAGTCGGTTTTGTAAAGATGAGTTAGACCCCAAATTTCAACAGTAAGGAACAAATTTAGAGGACTAAATTCTTAAAAATAAAAGAAAATTTAGAGGACTACAAGTACAAAATAAAAAACTAGTATATTTGATTTTTGCTTTTTGAACTGTGATATAACATTTACTTCTTTGTTAATCAGTCTCTATGGTGAAATTGATGTATTCATCGAACCCTTCACGGTGGTTAGTTTGCCATCATTAAAGACACTGCAAATCAGCATGGATTATGTGGAAGTGTCTAACGTGAATGCACTTCTTTGTGGCTGCCCCATCATTGAATCTCTTAATCTTAGTTTCTCTCCGCAAAGCTTGGACAAAGTTTGTGTCCCATCTTCCGTAAGAAGGCTGGAAATCGCCAATGAGAATGAAGTTGGGGTTTACCTTGAAATAAATGCGCCTGATCTTGAGTACCTTAACATTACCCGAATCACATTTGGCCAAGCTTTCAGCATGTACAACTTACACAATGTCGTGGAAGCGTATCTTGATGTCTTTCCCCAATCGTTTGGTTCTGTCATCCCTTTACACAACCTTCTTGGCGCTCTCTCTGGGACTAAACATCTGGTGTTGAATCCTTCAACAACCATGGTTAAGTTTTTTTATTTCTATTTTTTTAAAGAGTAATGCTATAGACAACCTCTCTAACACTCTCTCATATGCACTCCCTCAATAATTGGTCCACATCATCATATTTTAAAAACTCATTACCTAAGAACCGGCTATACTGGTTTACATGGAGAGAGAAAATACCCTTTTTTTTTTCTTTCACTTTAAAACAAAAAGAATTGACACAGGCCCTTCGTTCCCACTCACCACCTGAGAATCAAACACAATCTCATGGAAATTCTATTTTTTGTTGTACTTCTGATAAACTTCTTCATCAATTTAAAATAAATCTTTGAATATTCACAAAGAAAAGAGAAAACGGGTTCTTTGATTTCCCCAAAGAATCAGTTCTTTTTAAACGCTTCTCTCTCTAGATCTCTCTCCATAGTTATCTCATTTGCAGCAACCACATACCCATATAAACAAAATATAGTTGAATCTATCTTCTTCAATTTAATTCAGTCTCCATATTTGAAATTCCAACACTTTTAACGATTTTTGTTGAAGAATACAGCCTTGCCTGTTGACAAGGCGAGGCAATGATTTTAAAGACATTATTTTTTATTTTAAAATATATTAATCCAAATTGAAACAAACCGGTTGCCTGTTGCAAACATTTCAATAATAGCCACGTGGGCCAATCACTGCGCAAGTATTCAAAAGAGTGTGTTCAAGCGACTGCTCCTAGCATCGCTCTTTTTTAAAAGTTATTTTATGTTTACATCATCATTTATACAGTGTAAGTTAGTTAGTAACTGATAATAAACTAGTAACTAAGTATATCTCCAATAATCATGATTTATTAATATATAGATAGATATTGCTTTGTACTAATTACTACTTGATGGCGTGTACAAACAGTGGTTGCTTGGTGAACCCCATGATCTACTTTTTCAAGAATTTCAATATTTGATTCGCCTAGAGCTCAATCTTCCCGGGTTTAACTCCAATTCTCTGCTCACCTTGCTTCAGAAATGTCCCATGCTTCAAGCTCTAATAATTCAGAATGACAAGGTTTGTTTAATCCAACCTTAATTTGGTCAATTTAACTTTGATGTGAAATTAACCAATTTTTATGAATAGATTACACAAATTAGAAGTTGCATCTTATTGCACAGTATACCACTTAATATATAACAAGATTAATTCATTACTATGAATATTGCATCTTATTCCTTTTCAAAAAATGAATATTGCATCTTATTCAAGTCCTTTGTTCCCTGCAGGAACAGTCACCAGTTTTAGGATGGGCTACCCAGCCAAGTGTTCCGAATTGTCTCGTATCCCACCTGACCTTTATTCAATTTAAAGGATTTCAAGGATTTCCAGATGAGGTGTCATTTGTTGAGCATGTTTTGCAAAAAGGACTCGTTTTAAAGACTATGATTATTGCTGATTGTTCCTTGGACCTAAAGAAGAAGTACGAGAATCTCAAAACATTATTTGAAGTGCCAAGGGCCTCAGGAATGTGTCAACTTAAATTTGAATGA
Protein sequence

>MS.gene93083.t1

MASESKRQRKPEEEIEEDRISNLPDGVLLHILSRLPTKTAVTTGRLSRRWRYLWQHLSVLDFADESHGLDSPERLYGEIDVFIEPFTVVSLPSLKTLQISMDYVEVSNVNALLCGCPIIESLNLSFSPQSLDKVCVPSSVRRLEIANENEVGVYLEINAPDLEYLNITRITFGQAFSMYNLHNVVEAYLDVFPQSFGSVIPLHNLLGALSGTKHLVLNPSTTMWLLGEPHDLLFQEFQYLIRLELNLPGFNSNSLLTLLQKCPMLQALIIQNDKEQSPVLGWATQPSVPNCLVSHLTFIQFKGFQGFPDEVSFVEHVLQKGLVLKTMIIADCSLDLKKKYENLKTLFEVPRASGMCQLKFE