AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97954

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97954.t1 MTR_8g445780 75.104 241 60 0 75 315 150 390 6.02e-123 360
MS.gene97954.t1 MTR_8g014210 71.983 232 64 1 131 362 1 231 2.09e-112 327
MS.gene97954.t1 MTR_8g014260 62.718 287 106 1 77 362 165 451 8.35e-111 331
MS.gene97954.t1 MTR_8g014260 40.323 186 81 7 1 185 1 157 2.03e-21 95.5
MS.gene97954.t1 MTR_4g024710 61.806 288 107 3 77 362 61 347 8.15e-109 322
MS.gene97954.t1 MTR_4g024745 60.073 273 106 3 75 345 13 284 1.29e-93 281
MS.gene97954.t1 MTR_8g077460 42.627 373 183 9 16 362 8 375 1.43e-75 238
MS.gene97954.t1 MTR_2g083840 50.505 297 134 7 75 362 165 457 2.78e-75 240
MS.gene97954.t1 MTR_2g083840 56.140 57 24 1 7 62 10 66 1.38e-11 65.9
MS.gene97954.t1 MTR_8g062270 49.123 285 126 9 89 361 176 453 1.12e-71 230
MS.gene97954.t1 MTR_8g468810 50.336 298 136 7 75 362 168 463 1.64e-71 230
MS.gene97954.t1 MTR_8g468810 32.258 186 98 7 4 176 3 173 5.63e-12 67.0
MS.gene97954.t1 MTR_8g058970 46.407 334 153 11 53 362 146 477 9.37e-69 223
MS.gene97954.t1 MTR_8g058970 56.452 62 24 2 4 62 3 64 2.00e-11 65.5
MS.gene97954.t1 MTR_5g092960 49.458 277 121 6 101 362 190 462 4.64e-66 216
MS.gene97954.t1 MTR_8g467800 42.623 305 148 10 77 362 157 453 3.10e-59 198
MS.gene97954.t1 MTR_8g467800 56.716 67 25 1 4 66 2 68 1.24e-14 75.1
MS.gene97954.t1 MTR_8g468840 45.085 295 149 7 75 360 158 448 5.79e-57 192
MS.gene97954.t1 MTR_8g468840 58.824 51 21 0 17 67 13 63 1.57e-12 68.9
MS.gene97954.t1 MTR_4g032730 43.082 318 143 10 74 362 159 467 1.76e-56 191
MS.gene97954.t1 MTR_8g468760 42.230 296 129 7 75 361 156 418 8.53e-52 177
MS.gene97954.t1 MTR_8g468810 51.923 208 92 5 75 276 168 373 7.45e-47 163
MS.gene97954.t1 MTR_8g468810 32.258 186 98 7 4 176 3 173 5.16e-12 67.0
MS.gene97954.t1 MTR_8g062080 31.637 452 206 16 3 362 5 445 2.84e-45 161
MS.gene97954.t1 MTR_8g058965 39.414 307 161 10 75 362 156 456 1.38e-43 157
MS.gene97954.t1 MTR_8g058965 49.333 75 35 2 18 91 13 85 1.42e-12 68.9
MS.gene97954.t1 MTR_8g469020 36.482 307 171 10 75 362 150 451 8.36e-42 152
MS.gene97954.t1 MTR_8g469020 58.824 51 20 1 18 67 7 57 8.27e-11 63.5
MS.gene97954.t1 MTR_5g092960 52.688 186 78 3 101 277 190 374 2.81e-41 149
MS.gene97954.t1 MTR_8g014160 35.811 296 124 6 75 362 188 425 5.76e-41 149
MS.gene97954.t1 MTR_8g014160 63.158 57 20 1 14 69 37 93 1.58e-14 74.7
MS.gene97954.t1 MTR_8g062310 36.177 293 164 9 74 362 155 428 1.78e-37 140
MS.gene97954.t1 MTR_8g062010 38.225 293 160 10 78 362 151 430 2.62e-35 134
MS.gene97954.t1 MTR_8g062010 60.784 51 19 1 17 66 8 58 9.13e-13 69.3
MS.gene97954.t1 MTR_8g070950 33.221 298 176 10 74 362 151 434 1.45e-34 132
MS.gene97954.t1 MTR_8g070950 58.333 60 21 2 1 59 1 57 1.68e-12 68.6
MS.gene97954.t1 MTR_8g468810 53.846 143 65 1 75 217 168 309 5.77e-34 128
MS.gene97954.t1 MTR_8g468810 32.258 186 98 7 4 176 3 173 3.93e-12 67.0
MS.gene97954.t1 MTR_8g468810 53.846 143 65 1 75 217 168 309 6.93e-34 128
MS.gene97954.t1 MTR_8g468810 32.258 186 98 7 4 176 3 173 4.46e-12 66.6
MS.gene97954.t1 MTR_8g468810 53.846 143 65 1 75 217 168 309 9.99e-34 128
MS.gene97954.t1 MTR_8g468810 32.258 186 98 7 4 176 3 173 5.08e-12 66.6
MS.gene97954.t1 MTR_8g062300 34.194 310 169 8 75 362 181 477 6.37e-33 128
MS.gene97954.t1 MTR_4g050250 37.743 257 131 9 75 307 168 419 5.52e-28 114
MS.gene97954.t1 MTR_7g098510 27.446 419 219 15 1 362 1 391 1.36e-27 112
MS.gene97954.t1 MTR_8g014230 63.529 85 29 2 278 362 22 104 6.31e-27 103
MS.gene97954.t1 MTR_1089s0010 28.499 393 224 16 12 362 2 379 1.03e-25 107
MS.gene97954.t1 MTR_7g098480 26.923 416 221 12 1 361 1 388 2.61e-25 107
MS.gene97954.t1 MTR_2g086020 48.092 131 66 2 234 362 185 315 2.91e-25 104
MS.gene97954.t1 MTR_7g098570 28.571 406 224 17 1 358 2 389 4.99e-25 106
MS.gene97954.t1 MTR_5g084070 32.558 258 160 8 104 354 169 419 1.20e-23 101
MS.gene97954.t1 MTR_7g032380 27.049 366 226 9 18 362 11 356 1.60e-23 100
MS.gene97954.t1 MTR_5g021550 54.762 84 37 1 1 83 2 85 9.21e-22 90.9
MS.gene97954.t1 MTR_3g087260 26.991 452 221 14 15 361 9 456 2.56e-21 95.1
MS.gene97954.t1 MTR_7g088260 29.592 392 211 16 17 362 90 462 3.46e-21 94.7
MS.gene97954.t1 MTR_5g058100 28.682 387 221 15 18 361 9 383 4.18e-21 94.0
MS.gene97954.t1 MTR_8g467800 42.484 153 80 5 77 222 157 308 8.05e-21 92.4
MS.gene97954.t1 MTR_8g467800 56.716 67 25 1 4 66 2 68 4.90e-15 75.9
MS.gene97954.t1 MTR_5g008950 27.132 387 216 15 20 362 1 365 1.66e-20 92.0
MS.gene97954.t1 MTR_4g024770 60.606 66 25 1 1 66 8 72 3.03e-20 84.3
MS.gene97954.t1 MTR_5g073420 26.098 387 239 12 12 362 5 380 6.05e-20 90.5
MS.gene97954.t1 MTR_8g014180 71.930 57 14 1 3 59 4 58 7.20e-20 82.8
MS.gene97954.t1 MTR_3g099830 28.684 380 213 18 18 359 12 371 8.52e-20 90.1
MS.gene97954.t1 MTR_7g056420 28.611 360 225 12 17 354 8 357 4.02e-19 89.4
MS.gene97954.t1 MTR_7g056430 28.611 360 225 12 17 354 8 357 4.64e-19 89.4
MS.gene97954.t1 MTR_8g014290 71.154 52 15 0 12 63 4 55 2.14e-18 81.6
MS.gene97954.t1 MTR_5g080710 32.271 251 155 9 112 354 203 446 3.38e-18 85.9
MS.gene97954.t1 MTR_6g040270 27.062 388 216 17 20 359 10 378 4.16e-18 85.1
MS.gene97954.t1 MTR_8g467790 32.773 238 119 9 75 307 293 494 6.05e-18 85.5
MS.gene97954.t1 MTR_4g024720 58.904 73 26 2 1 71 2 72 6.09e-18 81.6
MS.gene97954.t1 MTR_0543s0010 25.867 375 238 13 17 361 85 449 2.56e-17 83.2
MS.gene97954.t1 MTR_7g023330 27.540 374 196 14 17 335 19 372 5.99e-17 82.0
MS.gene97954.t1 MTR_1g108800 27.461 386 182 15 18 359 8 339 3.65e-15 76.3
MS.gene97954.t1 MTR_3g108560 28.975 283 141 10 88 361 157 388 3.66e-15 76.6
MS.gene97954.t1 MTR_4g130200 25.735 408 207 16 17 362 19 392 5.79e-15 75.9
MS.gene97954.t1 MTR_7g007370 34.899 149 59 5 12 124 13 159 2.30e-14 73.6
MS.gene97954.t1 MTR_3g114200 51.515 66 30 1 3 66 2 67 1.48e-13 68.2
MS.gene97954.t1 MTR_1g038760 57.143 56 24 0 5 60 2 57 4.30e-13 70.1
MS.gene97954.t1 MTR_1g038760 57.143 56 24 0 5 60 2 57 4.58e-13 70.1
MS.gene97954.t1 MTR_1g041245 38.053 113 64 2 255 362 275 386 1.61e-12 68.6
MS.gene97954.t1 MTR_7g056447 32.934 167 101 6 17 175 8 171 2.20e-12 67.4
MS.gene97954.t1 MTR_5g039970 65.217 46 16 0 17 62 18 63 2.32e-12 67.8
MS.gene97954.t1 MTR_4g080773 30.000 200 116 5 5 181 6 204 2.37e-12 68.6
MS.gene97954.t1 MTR_4g080773 30.000 200 116 5 5 181 6 204 2.48e-12 68.2
MS.gene97954.t1 MTR_5g083890 64.583 48 17 0 17 64 5 52 2.90e-12 68.2
MS.gene97954.t1 MTR_3g105060 25.505 396 208 14 18 362 14 373 3.70e-12 67.4
MS.gene97954.t1 MTR_7g098590 46.479 71 36 1 1 71 1 69 5.32e-12 63.2
MS.gene97954.t1 MTR_3g115430 34.483 116 70 3 249 362 14 125 5.45e-12 62.8
MS.gene97954.t1 MTR_1g038710 28.622 283 168 12 74 329 152 427 1.11e-11 66.2
MS.gene97954.t1 MTR_7g007340 46.753 77 34 3 9 84 3 73 1.47e-11 65.5
MS.gene97954.t1 MTR_3g023470 53.704 54 25 0 7 60 4 57 1.60e-11 65.5
MS.gene97954.t1 MTR_2g082860 44.737 76 39 1 5 80 17 89 1.64e-11 61.6
MS.gene97954.t1 MTR_4g080813 62.222 45 17 0 18 62 19 63 2.08e-11 60.5
MS.gene97954.t1 MTR_3g048290 28.962 183 113 6 6 174 12 191 2.25e-11 65.5
MS.gene97954.t1 MTR_4g051640 50.877 57 28 0 4 60 16 72 2.61e-11 62.0
MS.gene97954.t1 MTR_2g007090 39.130 115 63 4 18 125 10 124 2.76e-11 63.9
MS.gene97954.t1 MTR_4g080787 63.043 46 17 0 17 62 18 63 2.83e-11 65.1
MS.gene97954.t1 MTR_5g039450 25.876 371 225 14 18 359 6 355 3.16e-11 64.3
MS.gene97954.t1 MTR_3g113782 45.161 62 34 0 3 64 2 63 3.47e-11 59.3
MS.gene97954.t1 MTR_4g080590 44.000 75 40 1 17 89 22 96 4.90e-11 63.9
MS.gene97954.t1 MTR_4g051460 57.447 47 20 0 17 63 4 50 5.55e-11 58.2
MS.gene97954.t1 MTR_4g092320 57.447 47 20 0 17 63 4 50 5.55e-11 58.2
MS.gene97954.t1 MTR_4g080590 44.000 75 40 1 17 89 22 96 6.04e-11 63.9
MS.gene97954.t1 MTR_2g007500 53.571 56 26 0 7 62 3 58 9.22e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97954.t1 AT5G38570 28.900 391 214 15 18 361 2 375 3.31e-27 110
MS.gene97954.t1 AT4G26340 29.932 294 181 12 81 362 139 419 5.54e-21 94.0
MS.gene97954.t1 AT3G50710 31.385 325 181 15 62 359 115 424 9.06e-19 87.4
MS.gene97954.t1 AT4G00160 30.592 304 173 16 81 362 155 442 2.77e-17 83.2
MS.gene97954.t1 AT5G56420 27.148 291 186 11 84 362 146 422 6.29e-16 79.0
MS.gene97954.t1 AT5G56420 27.148 291 186 11 84 362 146 422 6.29e-16 79.0
MS.gene97954.t1 AT5G56420 27.148 291 186 11 84 362 146 422 6.29e-16 79.0
MS.gene97954.t1 AT4G10400 25.952 289 186 11 81 361 140 408 6.34e-16 79.0
MS.gene97954.t1 AT4G10400 25.952 289 186 11 81 361 140 408 6.34e-16 79.0
MS.gene97954.t1 AT4G10400 25.952 289 186 11 81 361 140 408 6.34e-16 79.0
MS.gene97954.t1 AT5G56452 29.185 233 141 9 134 359 8 223 1.46e-15 75.5
MS.gene97954.t1 AT5G56820 29.195 298 171 13 81 359 147 423 1.68e-15 77.8
MS.gene97954.t1 AT5G56430 25.798 376 223 16 20 361 4 357 3.32e-15 76.3
MS.gene97954.t1 AT5G56452 29.787 235 138 9 134 359 8 224 3.82e-15 74.3
MS.gene97954.t1 AT1G78750 25.862 348 217 15 46 359 115 455 4.55e-15 76.6
MS.gene97954.t1 AT1G78750 25.862 348 217 15 46 359 115 455 4.55e-15 76.6
MS.gene97954.t1 AT1G32375 26.780 295 189 10 81 362 142 422 5.85e-15 76.3
MS.gene97954.t1 AT1G32375 26.780 295 189 10 81 362 142 422 5.85e-15 76.3
MS.gene97954.t1 AT5G56390 27.986 293 187 9 81 361 144 424 8.47e-15 75.5
MS.gene97954.t1 AT3G54910 28.826 281 174 13 91 362 110 373 1.58e-13 71.6
MS.gene97954.t1 AT3G54910 28.826 281 174 13 91 362 110 373 1.58e-13 71.6
MS.gene97954.t1 AT3G54910 28.826 281 174 13 91 362 110 373 1.58e-13 71.6
MS.gene97954.t1 AT3G52680 31.987 297 168 15 85 362 164 445 2.52e-13 71.2
MS.gene97954.t1 AT1G16930 25.828 302 179 11 89 359 159 446 3.37e-13 70.9
MS.gene97954.t1 AT1G16930 25.828 302 179 11 89 359 159 446 3.37e-13 70.9
MS.gene97954.t1 AT1G16930 25.828 302 179 11 89 359 159 446 3.37e-13 70.9
MS.gene97954.t1 AT5G56325 26.894 264 170 8 110 361 11 263 4.72e-13 68.9
MS.gene97954.t1 AT4G10420 25.086 291 192 10 81 361 47 321 1.76e-12 68.2
MS.gene97954.t1 AT3G49480 25.589 297 190 10 73 351 152 435 2.32e-12 68.2
MS.gene97954.t1 AT5G56380 28.065 310 175 13 82 361 147 438 4.01e-12 67.4
MS.gene97954.t1 AT5G56380 28.065 310 175 13 82 361 147 438 4.01e-12 67.4
MS.gene97954.t1 AT5G38590 28.352 261 154 11 87 332 144 386 4.20e-12 67.4
MS.gene97954.t1 AT5G56800 27.425 299 178 15 82 362 67 344 4.34e-12 67.0
MS.gene97954.t1 AT3G26930 28.289 304 188 12 80 362 149 443 5.10e-12 67.4
MS.gene97954.t1 AT3G26930 28.289 304 188 12 80 362 104 398 5.35e-12 67.0
MS.gene97954.t1 AT4G26350 26.190 336 199 13 60 362 109 428 7.26e-12 66.6
MS.gene97954.t1 AT3G49020 28.289 304 181 13 81 362 157 445 1.15e-11 66.2
MS.gene97954.t1 AT1G13780 23.232 297 206 7 83 359 158 452 3.14e-11 64.7
MS.gene97954.t1 AT5G56440 44.048 84 44 1 18 101 2 82 5.09e-11 64.3
MS.gene97954.t1 AT3G52690 29.310 290 174 13 89 361 22 297 5.75e-11 63.2

Find 97 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTCTTGGCGCTCTCTC+TGG 0.222591 8.4:+33426717 MS.gene97954:CDS
CGCCTAGAGCTCAATCTTCC+CGG 0.271991 8.4:+33427024 MS.gene97954:CDS
GCCAGTTGTAACGGCGGTTT+TGG 0.282218 8.4:-33425346 None:intergenic
TCTTCTTCGATTTCTTCTTC+TGG 0.286797 8.4:-33425267 None:intergenic
GAATGTCGTACTTCTTCTTT+AGG 0.305944 8.4:-33427520 None:intergenic
TCTTTGTTAATCAGTCTCTA+TGG 0.310114 8.4:+33426315 MS.gene97954:intron
ATCCCTTTACACAACCTTCT+TGG 0.322992 8.4:+33426705 MS.gene97954:CDS
AAATTCTTTAAATTGAATAA+AGG 0.358875 8.4:-33427402 None:intergenic
ACCAAAACCGCCGTTACAAC+TGG 0.366112 8.4:+33425345 MS.gene97954:CDS
AACATTACCCGAATCACATT+TGG 0.369562 8.4:+33426603 MS.gene97954:CDS
GATCAGGCACATTTATTTCA+AGG 0.384905 8.4:-33426569 None:intergenic
CGCCAAGAAGGTTGTGTAAA+GGG 0.386248 8.4:-33426707 None:intergenic
TTGGCACATCAGATAATGTT+TGG 0.386365 8.4:-33427544 None:intergenic
TCCAGCCTTCTTACGGAAGA+TGG 0.394716 8.4:-33426516 None:intergenic
ATTCAATTTAAAGAATTTCA+AGG 0.397138 8.4:+33427407 MS.gene97954:CDS
GATGTCTTTCCCCAATCGTT+TGG 0.397912 8.4:+33426675 MS.gene97954:CDS
ACCTTCTTGGCGCTCTCTCT+GGG 0.399525 8.4:+33426718 MS.gene97954:CDS
AGGGTAGCCCATTCTAAAAT+TGG 0.406314 8.4:-33427344 None:intergenic
AGCTTGGCCAAATGTGATTC+GGG 0.414911 8.4:-33426610 None:intergenic
GATTACCGCTGATAGTTCAT+TGG 0.421500 8.4:+33427496 MS.gene97954:CDS
AACCCCAACTTCATTCTCAT+TGG 0.421872 8.4:-33426544 None:intergenic
CAAACTTTGTCCAAGCTTTG+CGG 0.422685 8.4:-33426489 None:intergenic
CTGAATCCATCTAACCGTTC+GGG 0.436295 8.4:-33425450 None:intergenic
AAGCTTGGCCAAATGTGATT+CGG 0.437271 8.4:-33426611 None:intergenic
ATGTGATTCGGGTAATGTTA+AGG 0.445469 8.4:-33426599 None:intergenic
AACGTGAATGCACTTCTTTG+TGG 0.447771 8.4:+33426429 MS.gene97954:CDS
GGCGATTTCCAGCCTTCTTA+CGG 0.449202 8.4:-33426523 None:intergenic
CTTTAAATTGAATAAAGGTC+AGG 0.450396 8.4:-33427397 None:intergenic
TCTGATGTGCCAAGGGCCTC+AGG 0.454537 8.4:+33427554 MS.gene97954:CDS
AACTTAACCATGGTTGTTGA+AGG 0.457176 8.4:-33426762 None:intergenic
CAGGTGCGATACGAGACAAT+TGG 0.467374 8.4:-33427378 None:intergenic
CTCGCCGCTGGCGATACCTC+TGG 0.470009 8.4:+33425376 MS.gene97954:CDS
GACAATTGGGAACACTTGGC+AGG 0.470535 8.4:-33427364 None:intergenic
CCCATCTTCCGTAAGAAGGC+TGG 0.470819 8.4:+33426515 MS.gene97954:CDS
GATGACAGAACCAAACGATT+GGG 0.471945 8.4:-33426685 None:intergenic
TCTCTCTGGGACTAAACATC+TGG 0.472916 8.4:+33426731 MS.gene97954:CDS
ATCGCCAATGAGAATGAAGT+TGG 0.478040 8.4:+33426540 MS.gene97954:CDS
TCAACAAATGACACCTCATC+TGG 0.479159 8.4:-33427434 None:intergenic
TCGCCAATGAGAATGAAGTT+GGG 0.480417 8.4:+33426541 MS.gene97954:CDS
AGGTGCGATACGAGACAATT+GGG 0.480496 8.4:-33427377 None:intergenic
ATTTGCAGTGTCTTTAATGA+TGG 0.487972 8.4:-33426381 None:intergenic
ATGTTAAGGTACTCAAGATC+AGG 0.493784 8.4:-33426585 None:intergenic
GTGTCCCATCTTCCGTAAGA+AGG 0.494377 8.4:+33426511 MS.gene97954:CDS
TAGTTTCTCTCCGCAAAGCT+TGG 0.495255 8.4:+33426479 MS.gene97954:CDS
AGATACGCTTCCACGACATT+GGG 0.500275 8.4:-33426651 None:intergenic
AAGATACGCTTCCACGACAT+TGG 0.501628 8.4:-33426652 None:intergenic
CCAGCCTTCTTACGGAAGAT+GGG 0.510309 8.4:-33426515 None:intergenic
AGATTAAGAGATTCAATGAT+GGG 0.511116 8.4:-33426456 None:intergenic
GCAAATCAGCATGGATTATG+TGG 0.517789 8.4:+33426398 MS.gene97954:CDS
TGACACATTCCTGAGGCCCT+TGG 0.520521 8.4:-33427563 None:intergenic
ACCCGGGAAGATTGAGCTCT+AGG 0.521976 8.4:-33427026 None:intergenic
GCCTAGAGCTCAATCTTCCC+GGG 0.523790 8.4:+33427025 MS.gene97954:CDS
GGATGACAGAACCAAACGAT+TGG 0.529615 8.4:-33426686 None:intergenic
TTGGGAGACGTGATAGGATG+TGG 0.531172 8.4:-33425323 None:intergenic
ATTATTAGAACTTGAAGCAT+GGG 0.531928 8.4:-33427087 None:intergenic
AGTTGTACATGCTGAAAGCT+TGG 0.536926 8.4:-33426626 None:intergenic
AAGATTAAGAGATTCAATGA+TGG 0.540155 8.4:-33426457 None:intergenic
GCGCCAAGAAGGTTGTGTAA+AGG 0.540668 8.4:-33426708 None:intergenic
CAGAGAATTTGAGTTAAACC+CGG 0.544044 8.4:-33427043 None:intergenic
CGCCAATGAGAATGAAGTTG+GGG 0.556833 8.4:+33426542 MS.gene97954:CDS
ACGAGACAATTGGGAACACT+TGG 0.559054 8.4:-33427368 None:intergenic
TCCCAGAGAGAGCGCCAAGA+AGG 0.560841 8.4:-33426719 None:intergenic
TGGCAAACTAACCACGGTGG+AGG 0.560906 8.4:-33426361 None:intergenic
AGATAGACGGCCAGTTGTAA+CGG 0.562751 8.4:-33425355 None:intergenic
AATTATTAGAACTTGAAGCA+TGG 0.571659 8.4:-33427088 None:intergenic
GGCAAACTAACCACGGTGGA+GGG 0.575221 8.4:-33426360 None:intergenic
ATGTGGAGGAGTACACCGTC+TGG 0.576758 8.4:-33425306 None:intergenic
AAACATTATCTGATGTGCCA+AGG 0.577099 8.4:+33427546 MS.gene97954:CDS
AACATTATCTGATGTGCCAA+GGG 0.577206 8.4:+33427547 MS.gene97954:CDS
TAATCCATCATCAATGGCAT+CGG 0.581132 8.4:+33425224 None:intergenic
AGAGAATTTGAGTTAAACCC+GGG 0.586590 8.4:-33427042 None:intergenic
ATCGCCAGCGGCGAGATAGA+CGG 0.587569 8.4:-33425368 None:intergenic
CTGGCCGTCTATCTCGCCGC+TGG 0.598014 8.4:+33425364 MS.gene97954:CDS
ATCAAGTCCGTGAGATTCGT+CGG 0.601030 8.4:-33425424 None:intergenic
TTCGTCGGCGAAGTCGAGAA+CGG 0.610913 8.4:-33425409 None:intergenic
CATGGGACATTTCTGAAGCA+AGG 0.613005 8.4:-33427070 None:intergenic
GTTGAATCCTTCAACAACCA+TGG 0.615902 8.4:+33426755 MS.gene97954:CDS
ATGTACAAACAGTGGCTGCT+TGG 0.624830 8.4:+33426958 MS.gene97954:intron
GACTTCGCCGACGAATCTCA+CGG 0.628163 8.4:+33425417 MS.gene97954:CDS
GGAGACGTGATAGGATGTGG+AGG 0.628437 8.4:-33425320 None:intergenic
TCTAATAATTCAGAATGACA+AGG 0.629883 8.4:+33427101 MS.gene97954:CDS
CGAATCAGTAATTTACCAGA+CGG 0.632147 8.4:+33425291 MS.gene97954:CDS
ACAATTGGGAACACTTGGCA+GGG 0.634349 8.4:-33427363 None:intergenic
ATGACAGAACCAAACGATTG+GGG 0.634767 8.4:-33426684 None:intergenic
ATTTCAAGGAATTCCAGATG+AGG 0.637246 8.4:+33427421 MS.gene97954:CDS
AAAGACACTGCAAATCAGCA+TGG 0.640510 8.4:+33426389 MS.gene97954:CDS
GTACAACTTACCCAATGTCG+TGG 0.648965 8.4:+33426641 MS.gene97954:CDS
GATTAAGAGATTCAATGATG+GGG 0.667084 8.4:-33426455 None:intergenic
TAGGTCCAATGAACTATCAG+CGG 0.669674 8.4:-33427501 None:intergenic
TAATGATGGCAAACTAACCA+CGG 0.674383 8.4:-33426367 None:intergenic
GAACGGAGAGATGCTGCCAG+AGG 0.679033 8.4:-33425392 None:intergenic
GCTGCCAGAGGTATCGCCAG+CGG 0.682009 8.4:-33425380 None:intergenic
TTTAAGTTGACACATTCCTG+AGG 0.695966 8.4:-33427570 None:intergenic
TAGACGGCCAGTTGTAACGG+CGG 0.704524 8.4:-33425352 None:intergenic
ACGGACTTGATTCACCCGAA+CGG 0.705095 8.4:+33425436 MS.gene97954:CDS
TGATGGCAAACTAACCACGG+TGG 0.770956 8.4:-33426364 None:intergenic
ATTCATCGAACCCTCCACCG+TGG 0.776767 8.4:+33426350 MS.gene97954:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAAAGAAAATTTAG+AGG + chr8.4:33426223-33426242 MS.gene97954:intron 10.0%
!! AAATTCTTTAAATTGAATAA+AGG - chr8.4:33427405-33427424 None:intergenic 10.0%
!! ACATAATAATTAGAAATTGT+TGG + chr8.4:33425994-33426013 MS.gene97954:intron 15.0%
!! ATTCAATTTAAAGAATTTCA+AGG + chr8.4:33427407-33427426 MS.gene97954:CDS 15.0%
!!! AATTTTCTCATATATAAATC+TGG + chr8.4:33425765-33425784 MS.gene97954:intron 15.0%
!!! CAATATTCATTTTTTTGAAA+AGG - chr8.4:33427285-33427304 None:intergenic 15.0%
!! GAAATAAAAAAACTTAACCA+TGG - chr8.4:33426775-33426794 None:intergenic 20.0%
!! TATGAGTAAATCATGATTAT+TGG - chr8.4:33426893-33426912 None:intergenic 20.0%
!! TGTAATCTATTCATAAAAAC+TGG - chr8.4:33427178-33427197 None:intergenic 20.0%
!! TTAATCTTGCTAATATTAAG+TGG - chr8.4:33427234-33427253 None:intergenic 20.0%
! AAGATTAAGAGATTCAATGA+TGG - chr8.4:33426460-33426479 None:intergenic 25.0%
! AAGTTAAATTGACCAAATTC+AGG - chr8.4:33427141-33427160 None:intergenic 25.0%
! AATTATTAGAACTTGAAGCA+TGG - chr8.4:33427091-33427110 None:intergenic 25.0%
! AGATTAAGAGATTCAATGAT+GGG - chr8.4:33426459-33426478 None:intergenic 25.0%
! ATTATTAGAACTTGAAGCAT+GGG - chr8.4:33427090-33427109 None:intergenic 25.0%
! ATTCTTGAAAAAGTAGATCA+TGG - chr8.4:33426990-33427009 None:intergenic 25.0%
! CTTGATAAAAGTTTACAAAG+AGG + chr8.4:33426109-33426128 MS.gene97954:intron 25.0%
! CTTTAAATTGAATAAAGGTC+AGG - chr8.4:33427400-33427419 None:intergenic 25.0%
! GATTGAGCTTATGAAAAATA+TGG - chr8.4:33425952-33425971 None:intergenic 25.0%
! TCTAATAATTCAGAATGACA+AGG + chr8.4:33427101-33427120 MS.gene97954:CDS 25.0%
! TTCTTGAAAAAGTAGATCAT+GGG - chr8.4:33426989-33427008 None:intergenic 25.0%
!! CTTTTTCCTTCCATAAAATA+CGG - chr8.4:33425856-33425875 None:intergenic 25.0%
!!! CATAAGTTATTTTCCAAATG+AGG + chr8.4:33425926-33425945 MS.gene97954:intron 25.0%
ACACTTCTCACATTATAAGT+CGG + chr8.4:33426134-33426153 MS.gene97954:intron 30.0%
ACGGTAATGAACAAATTTAG+AGG + chr8.4:33426189-33426208 MS.gene97954:intron 30.0%
ATGAAAAATATGGCCTCATT+TGG - chr8.4:33425942-33425961 None:intergenic 30.0%
ATTATTATGTAACCCGACTA+TGG - chr8.4:33425984-33426003 None:intergenic 30.0%
ATTTGCAGTGTCTTTAATGA+TGG - chr8.4:33426384-33426403 None:intergenic 30.0%
GATTAAGAGATTCAATGATG+GGG - chr8.4:33426458-33426477 None:intergenic 30.0%
GTTCATTACCGTTGAAATTT+GGG - chr8.4:33426181-33426200 None:intergenic 30.0%
TAAATTGACCAAATTCAGGT+TGG - chr8.4:33427137-33427156 None:intergenic 30.0%
TCTTGAAAAAGTAGATCATG+GGG - chr8.4:33426988-33427007 None:intergenic 30.0%
TCTTTGTTAATCAGTCTCTA+TGG + chr8.4:33426315-33426334 MS.gene97954:intron 30.0%
TGTTCATTACCGTTGAAATT+TGG - chr8.4:33426182-33426201 None:intergenic 30.0%
TGTTTAATCCAACCTGAATT+TGG + chr8.4:33427126-33427145 MS.gene97954:intron 30.0%
TTCATAAGCTCAATCCAAAT+GGG + chr8.4:33425957-33425976 MS.gene97954:intron 30.0%
TTCATTACCGTTGAAATTTG+GGG - chr8.4:33426180-33426199 None:intergenic 30.0%
TTTCATAAGCTCAATCCAAA+TGG + chr8.4:33425956-33425975 MS.gene97954:intron 30.0%
! CAAGCTTTTACCGTATTTTA+TGG + chr8.4:33425843-33425862 MS.gene97954:intron 30.0%
! CTTTTACCGTATTTTATGGA+AGG + chr8.4:33425847-33425866 MS.gene97954:intron 30.0%
!! TACAGTTGCCAATTTTAGAA+TGG + chr8.4:33427336-33427355 MS.gene97954:CDS 30.0%
!! TATGCTTGTTATTACTGTTC+TGG + chr8.4:33425648-33425667 MS.gene97954:intron 30.0%
!!! ACAGTTGCCAATTTTAGAAT+GGG + chr8.4:33427337-33427356 MS.gene97954:CDS 30.0%
AAACATTATCTGATGTGCCA+AGG + chr8.4:33427546-33427565 MS.gene97954:CDS 35.0%
AACATTACCCGAATCACATT+TGG + chr8.4:33426603-33426622 MS.gene97954:CDS 35.0%
AACATTATCTGATGTGCCAA+GGG + chr8.4:33427547-33427566 MS.gene97954:CDS 35.0%
AACTTAACCATGGTTGTTGA+AGG - chr8.4:33426765-33426784 None:intergenic 35.0%
ACTGTACCTAAAAGAAACCA+AGG - chr8.4:33427322-33427341 None:intergenic 35.0%
AGTTAGACCCCAAATTTCAA+CGG + chr8.4:33426170-33426189 MS.gene97954:intron 35.0%
ATTTCAAGGAATTCCAGATG+AGG + chr8.4:33427421-33427440 MS.gene97954:CDS 35.0%
CGAATCAGTAATTTACCAGA+CGG + chr8.4:33425291-33425310 MS.gene97954:CDS 35.0%
GAATGTCGTACTTCTTCTTT+AGG - chr8.4:33427523-33427542 None:intergenic 35.0%
GATCAGGCACATTTATTTCA+AGG - chr8.4:33426572-33426591 None:intergenic 35.0%
TAATGATGGCAAACTAACCA+CGG - chr8.4:33426370-33426389 None:intergenic 35.0%
TAGTGTCACATCAGTTAGAT+AGG + chr8.4:33426083-33426102 MS.gene97954:intron 35.0%
TCATAAGCTCAATCCAAATG+GGG + chr8.4:33425958-33425977 MS.gene97954:intron 35.0%
TCTTCTTCGATTTCTTCTTC+TGG - chr8.4:33425270-33425289 None:intergenic 35.0%
TTGCATCTTATTCAAGTCCT+TGG + chr8.4:33427302-33427321 MS.gene97954:intron 35.0%
TTGGCACATCAGATAATGTT+TGG - chr8.4:33427547-33427566 None:intergenic 35.0%
! AACTTTATCGATTGAGTCGT+GGG + chr8.4:33425578-33425597 MS.gene97954:intron 35.0%
! AGAGAATTTGAGTTAAACCC+GGG - chr8.4:33427045-33427064 None:intergenic 35.0%
! ATGTGATTCGGGTAATGTTA+AGG - chr8.4:33426602-33426621 None:intergenic 35.0%
! ATGTTAAGGTACTCAAGATC+AGG - chr8.4:33426588-33426607 None:intergenic 35.0%
! CAGAGAATTTGAGTTAAACC+CGG - chr8.4:33427046-33427065 None:intergenic 35.0%
! TCAAGTCCTTGGTTTCTTTT+AGG + chr8.4:33427313-33427332 MS.gene97954:intron 35.0%
! TTGATGACATGTACAAACAG+TGG + chr8.4:33426950-33426969 MS.gene97954:intron 35.0%
! TTTTCGATTATGACCCTGTT+TGG + chr8.4:33425800-33425819 MS.gene97954:intron 35.0%
!! TTTAAGTTGACACATTCCTG+AGG - chr8.4:33427573-33427592 None:intergenic 35.0%
!!! GTTGAGCTTGTTTTGCAAAA+AGG + chr8.4:33427452-33427471 MS.gene97954:CDS 35.0%
AAAGACACTGCAAATCAGCA+TGG + chr8.4:33426389-33426408 MS.gene97954:CDS 40.0%
AACCCCAACTTCATTCTCAT+TGG - chr8.4:33426547-33426566 None:intergenic 40.0%
AACGTGAATGCACTTCTTTG+TGG + chr8.4:33426429-33426448 MS.gene97954:CDS 40.0%
AAGCCAAACAAGGAAACTAG+AGG - chr8.4:33426044-33426063 None:intergenic 40.0%
AAGCTTGGCCAAATGTGATT+CGG - chr8.4:33426614-33426633 None:intergenic 40.0%
AGGCAGATCACAATAAAGGT+GGG - chr8.4:33426024-33426043 None:intergenic 40.0%
AGTTGTACATGCTGAAAGCT+TGG - chr8.4:33426629-33426648 None:intergenic 40.0%
ATCCCTTTACACAACCTTCT+TGG + chr8.4:33426705-33426724 MS.gene97954:CDS 40.0%
ATCGCCAATGAGAATGAAGT+TGG + chr8.4:33426540-33426559 MS.gene97954:CDS 40.0%
ATGACAGAACCAAACGATTG+GGG - chr8.4:33426687-33426706 None:intergenic 40.0%
CAAACTTTGTCCAAGCTTTG+CGG - chr8.4:33426492-33426511 None:intergenic 40.0%
CTAGAGGCAGATCACAATAA+AGG - chr8.4:33426028-33426047 None:intergenic 40.0%
GATGACAGAACCAAACGATT+GGG - chr8.4:33426688-33426707 None:intergenic 40.0%
GATTACCGCTGATAGTTCAT+TGG + chr8.4:33427496-33427515 MS.gene97954:CDS 40.0%
GCATAAGCTCAATCCAAACA+GGG - chr8.4:33425816-33425835 None:intergenic 40.0%
GTTGAATCCTTCAACAACCA+TGG + chr8.4:33426755-33426774 MS.gene97954:CDS 40.0%
TAGGTCCAATGAACTATCAG+CGG - chr8.4:33427504-33427523 None:intergenic 40.0%
TCAACAAATGACACCTCATC+TGG - chr8.4:33427437-33427456 None:intergenic 40.0%
TCGCCAATGAGAATGAAGTT+GGG + chr8.4:33426541-33426560 MS.gene97954:CDS 40.0%
TCTGAATCCATCTAACCGTT+CGG - chr8.4:33425454-33425473 None:intergenic 40.0%
TGCATAAGCTCAATCCAAAC+AGG - chr8.4:33425817-33425836 None:intergenic 40.0%
! GAACTTTATCGATTGAGTCG+TGG + chr8.4:33425577-33425596 MS.gene97954:intron 40.0%
! GCAAATCAGCATGGATTATG+TGG + chr8.4:33426398-33426417 MS.gene97954:CDS 40.0%
!! AAGTGAAGAGTGTTTGAGGT+AGG - chr8.4:33425680-33425699 None:intergenic 40.0%
!! AGGGTAGCCCATTCTAAAAT+TGG - chr8.4:33427347-33427366 None:intergenic 40.0%
!! CTTGTTTGGCTTGACTTTGA+GGG + chr8.4:33426052-33426071 MS.gene97954:intron 40.0%
!! TTGTTTGGCTTGACTTTGAG+GGG + chr8.4:33426053-33426072 MS.gene97954:intron 40.0%
!!! TTCTGGTTTTCGTTGTCGTT+TGG - chr8.4:33425253-33425272 None:intergenic 40.0%
AAGATACGCTTCCACGACAT+TGG - chr8.4:33426655-33426674 None:intergenic 45.0%
AATCCAAATGGGGCCATAGT+CGG + chr8.4:33425968-33425987 MS.gene97954:intron 45.0%
ACAATTGGGAACACTTGGCA+GGG - chr8.4:33427366-33427385 None:intergenic 45.0%
ACGAGACAATTGGGAACACT+TGG - chr8.4:33427371-33427390 None:intergenic 45.0%
AGATACGCTTCCACGACATT+GGG - chr8.4:33426654-33426673 None:intergenic 45.0%
AGATAGACGGCCAGTTGTAA+CGG - chr8.4:33425358-33425377 None:intergenic 45.0%
AGCTTGGCCAAATGTGATTC+GGG - chr8.4:33426613-33426632 None:intergenic 45.0%
AGGTGCGATACGAGACAATT+GGG - chr8.4:33427380-33427399 None:intergenic 45.0%
ATCAAGTCCGTGAGATTCGT+CGG - chr8.4:33425427-33425446 None:intergenic 45.0%
ATGTACAAACAGTGGCTGCT+TGG + chr8.4:33426958-33426977 MS.gene97954:intron 45.0%
CCTCAAAGTCAAGCCAAACA+AGG - chr8.4:33426054-33426073 None:intergenic 45.0%
CGCCAAGAAGGTTGTGTAAA+GGG - chr8.4:33426710-33426729 None:intergenic 45.0%
CGCCAATGAGAATGAAGTTG+GGG + chr8.4:33426542-33426561 MS.gene97954:CDS 45.0%
CTGAATCCATCTAACCGTTC+GGG - chr8.4:33425453-33425472 None:intergenic 45.0%
GAGGCAGATCACAATAAAGG+TGG - chr8.4:33426025-33426044 None:intergenic 45.0%
GATGTCTTTCCCCAATCGTT+TGG + chr8.4:33426675-33426694 MS.gene97954:CDS 45.0%
GGATGACAGAACCAAACGAT+TGG - chr8.4:33426689-33426708 None:intergenic 45.0%
GTACAACTTACCCAATGTCG+TGG + chr8.4:33426641-33426660 MS.gene97954:CDS 45.0%
TAGTTTCTCTCCGCAAAGCT+TGG + chr8.4:33426479-33426498 MS.gene97954:CDS 45.0%
TCCATAATCCACGTGGCATT+AGG + chr8.4:33425511-33425530 MS.gene97954:intron 45.0%
! AATGCCACGTGGATTATGGA+GGG - chr8.4:33425511-33425530 None:intergenic 45.0%
! CTGCCTCTAGTTTCCTTGTT+TGG + chr8.4:33426038-33426057 MS.gene97954:intron 45.0%
! TAATGCCACGTGGATTATGG+AGG - chr8.4:33425512-33425531 None:intergenic 45.0%
! TCCTAATGCCACGTGGATTA+TGG - chr8.4:33425515-33425534 None:intergenic 45.0%
!! CATGGGACATTTCTGAAGCA+AGG - chr8.4:33427073-33427092 None:intergenic 45.0%
!! CCTTGTTTGGCTTGACTTTG+AGG + chr8.4:33426051-33426070 MS.gene97954:intron 45.0%
!! GAGGAAGTGAAGAGTGTTTG+AGG - chr8.4:33425684-33425703 None:intergenic 45.0%
!! TCTCTCTGGGACTAAACATC+TGG + chr8.4:33426731-33426750 MS.gene97954:CDS 45.0%
!! TGTTTGGCTTGACTTTGAGG+GGG + chr8.4:33426054-33426073 MS.gene97954:intron 45.0%
!! TTTTGGTTGGGAGACGTGAT+AGG - chr8.4:33425332-33425351 None:intergenic 45.0%
!!! TTGTAACGGCGGTTTTGGTT+GGG - chr8.4:33425344-33425363 None:intergenic 45.0%
AAGCTCGAGTTCCTCAAGGT+AGG - chr8.4:33425622-33425641 None:intergenic 50.0%
ACCAAAACCGCCGTTACAAC+TGG + chr8.4:33425345-33425364 MS.gene97954:CDS 50.0%
ACGGACTTGATTCACCCGAA+CGG + chr8.4:33425436-33425455 MS.gene97954:CDS 50.0%
AGCTCGAGTTCCTCAAGGTA+GGG - chr8.4:33425621-33425640 None:intergenic 50.0%
ATCCAAATGGGGCCATAGTC+GGG + chr8.4:33425969-33425988 MS.gene97954:intron 50.0%
ATGCATGCCTGAGTGATACG+AGG - chr8.4:33425710-33425729 None:intergenic 50.0%
CAGGTGCGATACGAGACAAT+TGG - chr8.4:33427381-33427400 None:intergenic 50.0%
CCAGCCTTCTTACGGAAGAT+GGG - chr8.4:33426518-33426537 None:intergenic 50.0%
CCTCTAACCTCGTATCACTC+AGG + chr8.4:33425700-33425719 MS.gene97954:intron 50.0%
CCTGAGTGATACGAGGTTAG+AGG - chr8.4:33425703-33425722 None:intergenic 50.0%
CTCATCTTCCTAATGCCACG+TGG - chr8.4:33425522-33425541 None:intergenic 50.0%
GACAATTGGGAACACTTGGC+AGG - chr8.4:33427367-33427386 None:intergenic 50.0%
GAGTAAGCTCGAGTTCCTCA+AGG - chr8.4:33425626-33425645 None:intergenic 50.0%
GATTCACCCGAACGGTTAGA+TGG + chr8.4:33425444-33425463 MS.gene97954:intron 50.0%
GCGCCAAGAAGGTTGTGTAA+AGG - chr8.4:33426711-33426730 None:intergenic 50.0%
GGCGATTTCCAGCCTTCTTA+CGG - chr8.4:33426526-33426545 None:intergenic 50.0%
GTGTCCCATCTTCCGTAAGA+AGG + chr8.4:33426511-33426530 MS.gene97954:CDS 50.0%
TCCAGCCTTCTTACGGAAGA+TGG - chr8.4:33426519-33426538 None:intergenic 50.0%
TGATGGCAAACTAACCACGG+TGG - chr8.4:33426367-33426386 None:intergenic 50.0%
TTCCTCAAGGTAGGGTCCAA+TGG - chr8.4:33425613-33425632 None:intergenic 50.0%
TTGGGAGACGTGATAGGATG+TGG - chr8.4:33425326-33425345 None:intergenic 50.0%
!! GTTGTAACGGCGGTTTTGGT+TGG - chr8.4:33425345-33425364 None:intergenic 50.0%
AACCCGACTATGGCCCCATT+TGG - chr8.4:33425974-33425993 None:intergenic 55.0%
AACCTTCTTGGCGCTCTCTC+TGG + chr8.4:33426717-33426736 MS.gene97954:CDS 55.0%
ACCCGGGAAGATTGAGCTCT+AGG - chr8.4:33427029-33427048 None:intergenic 55.0%
ACCTTCTTGGCGCTCTCTCT+GGG + chr8.4:33426718-33426737 MS.gene97954:CDS 55.0%
ATTCATCGAACCCTCCACCG+TGG + chr8.4:33426350-33426369 MS.gene97954:CDS 55.0%
CCCATCTTCCGTAAGAAGGC+TGG + chr8.4:33426515-33426534 MS.gene97954:CDS 55.0%
CGCCTAGAGCTCAATCTTCC+CGG + chr8.4:33427024-33427043 MS.gene97954:CDS 55.0%
CTCAAGGTAGGGTCCAATGG+CGG - chr8.4:33425610-33425629 None:intergenic 55.0%
CTCTCCCTCCATAATCCACG+TGG + chr8.4:33425504-33425523 MS.gene97954:intron 55.0%
GACTTCGCCGACGAATCTCA+CGG + chr8.4:33425417-33425436 MS.gene97954:CDS 55.0%
GCCTAGAGCTCAATCTTCCC+GGG + chr8.4:33427025-33427044 MS.gene97954:CDS 55.0%
GGAGACGTGATAGGATGTGG+AGG - chr8.4:33425323-33425342 None:intergenic 55.0%
GGCAAACTAACCACGGTGGA+GGG - chr8.4:33426363-33426382 None:intergenic 55.0%
TAGACGGCCAGTTGTAACGG+CGG - chr8.4:33425355-33425374 None:intergenic 55.0%
TGACACATTCCTGAGGCCCT+TGG - chr8.4:33427566-33427585 None:intergenic 55.0%
TGGCAAACTAACCACGGTGG+AGG - chr8.4:33426364-33426383 None:intergenic 55.0%
TTCGTCGGCGAAGTCGAGAA+CGG - chr8.4:33425412-33425431 None:intergenic 55.0%
! ATGTGGAGGAGTACACCGTC+TGG - chr8.4:33425309-33425328 None:intergenic 55.0%
! CACGTGGATTATGGAGGGAG+AGG - chr8.4:33425506-33425525 None:intergenic 55.0%
! GCCAGTTGTAACGGCGGTTT+TGG - chr8.4:33425349-33425368 None:intergenic 55.0%
! TTGGCTTGACTTTGAGGGGG+TGG + chr8.4:33426057-33426076 MS.gene97954:intron 55.0%
ATCGCCAGCGGCGAGATAGA+CGG - chr8.4:33425371-33425390 None:intergenic 60.0%
CGCCATTGGACCCTACCTTG+AGG + chr8.4:33425608-33425627 MS.gene97954:intron 60.0%
GAACGGAGAGATGCTGCCAG+AGG - chr8.4:33425395-33425414 None:intergenic 60.0%
TCTGATGTGCCAAGGGCCTC+AGG + chr8.4:33427554-33427573 MS.gene97954:CDS 60.0%
!! TCCCAGAGAGAGCGCCAAGA+AGG - chr8.4:33426722-33426741 None:intergenic 60.0%
TCGTGGGTTCGTGCCGCCAT+TGG + chr8.4:33425594-33425613 MS.gene97954:intron 65.0%
!! GCTGCCAGAGGTATCGCCAG+CGG - chr8.4:33425383-33425402 None:intergenic 65.0%
CTCGCCGCTGGCGATACCTC+TGG + chr8.4:33425376-33425395 MS.gene97954:CDS 70.0%
CTGGCCGTCTATCTCGCCGC+TGG + chr8.4:33425364-33425383 MS.gene97954:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 33425237 33427601 33425237 ID=MS.gene97954
chr8.4 mRNA 33425237 33427601 33425237 ID=MS.gene97954.t1;Parent=MS.gene97954
chr8.4 exon 33425237 33425457 33425237 ID=MS.gene97954.t1.exon1;Parent=MS.gene97954.t1
chr8.4 CDS 33425237 33425457 33425237 ID=cds.MS.gene97954.t1;Parent=MS.gene97954.t1
chr8.4 exon 33426329 33426776 33426329 ID=MS.gene97954.t1.exon2;Parent=MS.gene97954.t1
chr8.4 CDS 33426329 33426776 33426329 ID=cds.MS.gene97954.t1;Parent=MS.gene97954.t1
chr8.4 exon 33426970 33427122 33426970 ID=MS.gene97954.t1.exon3;Parent=MS.gene97954.t1
chr8.4 CDS 33426970 33427122 33426970 ID=cds.MS.gene97954.t1;Parent=MS.gene97954.t1
chr8.4 exon 33427335 33427601 33427335 ID=MS.gene97954.t1.exon4;Parent=MS.gene97954.t1
chr8.4 CDS 33427335 33427601 33427335 ID=cds.MS.gene97954.t1;Parent=MS.gene97954.t1
Gene Sequence

>MS.gene97954

ATGGCATCGGAATCCAAACGACAACGAAAACCAGAAGAAGAAATCGAAGAAGATCGAATCAGTAATTTACCAGACGGTGTACTCCTCCACATCCTATCACGTCTCCCAACCAAAACCGCCGTTACAACTGGCCGTCTATCTCGCCGCTGGCGATACCTCTGGCAGCATCTCTCCGTTCTCGACTTCGCCGACGAATCTCACGGACTTGATTCACCCGAACGGTTAGATGGATTCAGAAGTTTCTCTGTTTTAGTTAACAGCGTCTTCCTCTCCCTCCATAATCCACGTGGCATTAGGAAGATGAGTCTCAACTGTCAGTCTCTTTTTTATGATAAGTGTCGAACTTTATCGATTGAGTCGTGGGTTCGTGCCGCCATTGGACCCTACCTTGAGGAACTCGAGCTTACTCTATATGCTTGTTATTACTGTTCTGGATTCAACCTACCTCAAACACTCTTCACTTCCTCTAACCTCGTATCACTCAGGCATGCATGCATAACGTTCAACTAATTGTTTTTTCGTCGACTTAATTTTCTCATATATAAATCTGGAGTGAGTGTTTTTTTTCGATTATGACCCTGTTTGGATTGAGCTTATGCAGATAAACAAGCTTTTACCGTATTTTATGGAAGGAAAAAGAAAATATTTTCATGAGAGCTTATGAATTAATATAAAAACTTATTTATTTGCATAAGTTATTTTCCAAATGAGGCCATATTTTTCATAAGCTCAATCCAAATGGGGCCATAGTCGGGTTACATAATAATTAGAAATTGTTGGTAATCCCACCTTTATTGTGATCTGCCTCTAGTTTCCTTGTTTGGCTTGACTTTGAGGGGGTGGATTTAGTGTCACATCAGTTAGATAGGACGCTTGATAAAAGTTTACAAAGAGGAGACACTTCTCACATTATAAGTCGGTTTTGTAAAGATGAGTTAGACCCCAAATTTCAACGGTAATGAACAAATTTAGAGGACTAAATTCTTAAAAATAAAAGAAAATTTAGAGGACTACAAGTACAAAATAAAAAATTACTATATTTGATTTTTGCTTTTTGAACTGTGATATAACATTTACTTCTTTGTTAATCAGTCTCTATGGTGAAATTGATGTATTCATCGAACCCTCCACCGTGGTTAGTTTGCCATCATTAAAGACACTGCAAATCAGCATGGATTATGTGGAAGTGTCTAACGTGAATGCACTTCTTTGTGGCTGCCCCATCATTGAATCTCTTAATCTTAGTTTCTCTCCGCAAAGCTTGGACAAAGTTTGTGTCCCATCTTCCGTAAGAAGGCTGGAAATCGCCAATGAGAATGAAGTTGGGGTTTACCTTGAAATAAATGTGCCTGATCTTGAGTACCTTAACATTACCCGAATCACATTTGGCCAAGCTTTCAGCATGTACAACTTACCCAATGTCGTGGAAGCGTATCTTGATGTCTTTCCCCAATCGTTTGGTTCTGTCATCCCTTTACACAACCTTCTTGGCGCTCTCTCTGGGACTAAACATCTGGTGTTGAATCCTTCAACAACCATGGTTAAGTTTTTTTATTTCTATTTTTTTTAAAAGTTATTTTATGTTCACATCATCATTTATACAGAGTATAAGTTAGTAAGCAACTGATAATAAACTAGTAACTAAGTATATCTCCAATAATCATGATTTACTCATATATAGATAGATATTGTTTTGTACTAATTAATTACTACTTGATGACATGTACAAACAGTGGCTGCTTGGTGAACCCCATGATCTACTTTTTCAAGAATTTCAATATTTGCTTCGCCTAGAGCTCAATCTTCCCGGGTTTAACTCAAATTCTCTGCTCACCTTGCTTCAGAAATGTCCCATGCTTCAAGTTCTAATAATTCAGAATGACAAGGTTTGTTTAATCCAACCTGAATTTGGTCAATTTAACTTTGATGTGAAATTAACCAGTTTTTATGAATAGATTACACAAATTAGAAGTTGCATCTTATTGCACAGTATACCACTTAATATTAGCAAGATTAATTCATTACTATGAATATTGCATCTTATTCCTTTTCAAAAAAATGAATATTGCATCTTATTCAAGTCCTTGGTTTCTTTTAGGTACAGTTGCCAATTTTAGAATGGGCTACCCTGCCAAGTGTTCCCAATTGTCTCGTATCGCACCTGACCTTTATTCAATTTAAAGAATTTCAAGGAATTCCAGATGAGGTGTCATTTGTTGAGCTTGTTTTGCAAAAAGGACTCGTTTTAAAGACAATGATGATTACCGCTGATAGTTCATTGGACCTAAAGAAGAAGTACGACATTCTCCAAACATTATCTGATGTGCCAAGGGCCTCAGGAATGTGTCAACTTAAATTTGAATGA
Protein sequence

>MS.gene97954.t1

MASESKRQRKPEEEIEEDRISNLPDGVLLHILSRLPTKTAVTTGRLSRRWRYLWQHLSVLDFADESHGLDSPERLYGEIDVFIEPSTVVSLPSLKTLQISMDYVEVSNVNALLCGCPIIESLNLSFSPQSLDKVCVPSSVRRLEIANENEVGVYLEINVPDLEYLNITRITFGQAFSMYNLPNVVEAYLDVFPQSFGSVIPLHNLLGALSGTKHLVLNPSTTMWLLGEPHDLLFQEFQYLLRLELNLPGFNSNSLLTLLQKCPMLQVLIIQNDKVQLPILEWATLPSVPNCLVSHLTFIQFKEFQGIPDEVSFVELVLQKGLVLKTMMITADSSLDLKKKYDILQTLSDVPRASGMCQLKFE