AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97956

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97956.t1 MTR_8g445780 76.279 215 51 0 1 215 176 390 5.77e-114 333
MS.gene97956.t1 MTR_8g014210 71.983 232 64 1 31 262 1 231 1.28e-113 326
MS.gene97956.t1 MTR_8g014260 65.251 259 90 0 4 262 193 451 3.51e-107 318
MS.gene97956.t1 MTR_4g024710 63.985 261 92 2 3 262 88 347 1.83e-105 310
MS.gene97956.t1 MTR_4g024745 62.705 244 89 2 3 245 42 284 4.24e-91 271
MS.gene97956.t1 MTR_8g062270 48.897 272 121 8 1 261 189 453 4.24e-70 223
MS.gene97956.t1 MTR_2g083840 50.929 269 119 7 3 262 193 457 1.62e-68 219
MS.gene97956.t1 MTR_5g092960 49.458 277 121 6 1 262 190 462 1.74e-67 216
MS.gene97956.t1 MTR_8g468810 49.442 269 124 7 4 262 197 463 1.18e-63 206
MS.gene97956.t1 MTR_8g058970 49.265 272 123 9 4 262 208 477 1.25e-60 199
MS.gene97956.t1 MTR_8g468840 47.104 259 126 6 9 260 194 448 8.97e-58 191
MS.gene97956.t1 MTR_8g467800 44.043 277 129 9 4 262 185 453 1.23e-55 185
MS.gene97956.t1 MTR_4g032730 43.253 289 127 9 2 262 188 467 3.99e-53 179
MS.gene97956.t1 MTR_8g077460 63.014 146 53 1 117 262 231 375 8.20e-53 176
MS.gene97956.t1 MTR_8g468760 42.322 267 112 7 4 261 185 418 5.15e-48 164
MS.gene97956.t1 MTR_5g092960 52.688 186 78 3 1 177 190 374 2.50e-42 149
MS.gene97956.t1 MTR_8g058965 42.636 258 136 8 12 262 204 456 3.97e-42 150
MS.gene97956.t1 MTR_8g468810 50.838 179 80 5 4 176 197 373 7.98e-39 139
MS.gene97956.t1 MTR_8g469020 37.037 270 155 8 4 262 186 451 1.44e-38 140
MS.gene97956.t1 MTR_8g062080 36.364 264 140 9 11 262 198 445 5.67e-38 138
MS.gene97956.t1 MTR_8g014160 36.194 268 105 6 3 262 216 425 1.39e-37 137
MS.gene97956.t1 MTR_8g062310 35.741 263 149 7 4 262 182 428 4.76e-35 130
MS.gene97956.t1 MTR_8g070950 33.579 271 149 7 6 262 181 434 6.18e-33 124
MS.gene97956.t1 MTR_8g062010 37.918 269 143 9 4 262 176 430 1.19e-32 124
MS.gene97956.t1 MTR_8g062300 35.036 274 144 7 11 262 216 477 3.96e-31 120
MS.gene97956.t1 MTR_8g014230 63.529 85 29 2 178 262 22 104 1.07e-27 102
MS.gene97956.t1 MTR_2g086020 48.092 131 66 2 134 262 185 315 2.46e-26 105
MS.gene97956.t1 MTR_8g468810 52.174 115 54 1 4 118 197 310 6.34e-26 103
MS.gene97956.t1 MTR_8g468810 52.174 115 54 1 4 118 197 310 7.56e-26 103
MS.gene97956.t1 MTR_8g468810 52.174 115 54 1 4 118 197 310 8.62e-26 104
MS.gene97956.t1 MTR_4g050250 38.318 214 116 7 6 207 210 419 1.36e-25 104
MS.gene97956.t1 MTR_5g084070 32.946 258 159 8 4 254 169 419 1.40e-25 104
MS.gene97956.t1 MTR_5g080710 32.669 251 154 9 12 254 203 446 1.27e-19 88.2
MS.gene97956.t1 MTR_8g467800 45.600 125 61 4 4 122 185 308 3.22e-17 80.5
MS.gene97956.t1 MTR_8g467790 30.660 212 109 7 1 207 316 494 8.09e-14 71.2
MS.gene97956.t1 MTR_7g098510 28.636 220 126 7 57 262 189 391 1.91e-13 69.7
MS.gene97956.t1 MTR_3g108560 30.841 214 124 6 56 261 191 388 4.84e-13 68.6
MS.gene97956.t1 MTR_5g008950 27.778 234 133 8 57 262 140 365 7.34e-13 68.2
MS.gene97956.t1 MTR_1g041245 38.053 113 64 2 155 262 275 386 8.59e-13 67.8
MS.gene97956.t1 MTR_3g115430 34.483 116 70 3 149 262 14 125 1.44e-12 63.5
MS.gene97956.t1 MTR_7g032380 31.538 130 84 3 136 262 229 356 2.54e-12 66.2
MS.gene97956.t1 MTR_7g098570 33.793 145 82 5 123 258 250 389 2.55e-12 66.6
MS.gene97956.t1 MTR_3g087260 26.421 299 171 8 9 261 161 456 4.10e-12 65.9
MS.gene97956.t1 MTR_7g098480 27.692 195 119 5 72 261 211 388 1.21e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97956.t1 AT5G38570 28.058 278 154 10 3 261 125 375 2.00e-18 84.0
MS.gene97956.t1 AT4G26340 28.938 273 170 11 1 262 160 419 1.52e-17 82.0
MS.gene97956.t1 AT3G50710 32.246 276 155 12 1 259 164 424 3.40e-17 80.9
MS.gene97956.t1 AT5G56452 28.151 238 147 9 29 259 3 223 9.28e-16 74.7
MS.gene97956.t1 AT5G56452 28.750 240 144 9 29 259 3 224 2.22e-15 73.6
MS.gene97956.t1 AT5G56420 26.255 259 157 9 8 262 194 422 1.43e-14 73.2
MS.gene97956.t1 AT5G56420 26.255 259 157 9 8 262 194 422 1.43e-14 73.2
MS.gene97956.t1 AT5G56420 26.255 259 157 9 8 262 194 422 1.43e-14 73.2
MS.gene97956.t1 AT5G56325 26.894 264 170 8 10 261 11 263 7.75e-14 70.1
MS.gene97956.t1 AT1G78750 25.746 268 174 10 11 259 194 455 1.21e-12 67.8
MS.gene97956.t1 AT1G78750 25.746 268 174 10 11 259 194 455 1.21e-12 67.8
MS.gene97956.t1 AT3G49020 29.710 276 158 12 8 262 185 445 4.92e-12 65.9
MS.gene97956.t1 AT1G16930 25.436 287 170 10 3 259 174 446 5.89e-12 65.5
MS.gene97956.t1 AT1G16930 25.436 287 170 10 3 259 174 446 5.89e-12 65.5
MS.gene97956.t1 AT1G16930 25.436 287 170 10 3 259 174 446 5.89e-12 65.5
MS.gene97956.t1 AT1G32375 25.940 266 171 9 9 262 171 422 1.18e-11 64.7
MS.gene97956.t1 AT1G32375 25.940 266 171 9 9 262 171 422 1.18e-11 64.7
MS.gene97956.t1 AT3G26930 28.369 282 173 11 1 262 126 398 1.31e-11 64.3
MS.gene97956.t1 AT3G26930 28.369 282 173 11 1 262 171 443 1.34e-11 64.7
MS.gene97956.t1 AT3G26920 29.348 276 165 13 1 261 79 339 1.74e-11 63.9
MS.gene97956.t1 AT3G26920 29.348 276 165 13 1 261 79 339 1.74e-11 63.9
MS.gene97956.t1 AT5G56380 27.931 290 162 12 1 261 167 438 2.89e-11 63.5
MS.gene97956.t1 AT5G56380 27.931 290 162 12 1 261 167 438 2.89e-11 63.5
MS.gene97956.t1 AT3G49480 25.385 260 164 9 11 253 51 297 4.43e-11 62.4
MS.gene97956.t1 AT3G49480 25.681 257 161 9 12 251 192 435 4.89e-11 62.8
MS.gene97956.t1 AT5G44850 26.190 252 131 10 34 259 106 328 5.03e-11 62.4
MS.gene97956.t1 AT5G56440 27.174 276 163 9 9 261 169 429 5.22e-11 62.8

Find 70 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTCTTGGCGCTCTCTC+TGG 0.222591 8.4:+33394852 MS.gene97956:CDS
CGCCTAGAGCTCAATCTTCC+CGG 0.271991 8.4:+33395159 MS.gene97956:CDS
GAATGTCGTACTTCTTCTTT+AGG 0.305944 8.4:-33395655 None:intergenic
ATCCCTTTACACAACCTTCT+TGG 0.322992 8.4:+33394840 MS.gene97956:CDS
AAATTCTTTAAATTGAATAA+AGG 0.358875 8.4:-33395537 None:intergenic
AACATTACCCGAATCACATT+TGG 0.369562 8.4:+33394738 MS.gene97956:CDS
CGCCAAGAAGGTTGTGTAAA+GGG 0.386248 8.4:-33394842 None:intergenic
TTGGCACATCAGATAATGTT+TGG 0.386365 8.4:-33395679 None:intergenic
TCCAGCCTTCTTACGGAAGA+TGG 0.394716 8.4:-33394651 None:intergenic
ATTCAATTTAAAGAATTTCA+AGG 0.397138 8.4:+33395542 MS.gene97956:CDS
GATGTCTTTCCCCAATCGTT+TGG 0.397912 8.4:+33394810 MS.gene97956:CDS
ACCTTCTTGGCGCTCTCTCT+GGG 0.399525 8.4:+33394853 MS.gene97956:CDS
GATCAGGCGCATTTATTTCA+AGG 0.403961 8.4:-33394704 None:intergenic
AGGGTAGCCCATTCTAAAAT+TGG 0.406314 8.4:-33395479 None:intergenic
AGCTTGGCCAAATGTGATTC+GGG 0.414911 8.4:-33394745 None:intergenic
GATTACCGCTGATAGTTCAT+TGG 0.421500 8.4:+33395631 MS.gene97956:CDS
CAAACTTTGTCCAAGCTTTG+CGG 0.422685 8.4:-33394624 None:intergenic
AAGCTTGGCCAAATGTGATT+CGG 0.437271 8.4:-33394746 None:intergenic
ATGTGATTCGGGTAATGTTA+AGG 0.445469 8.4:-33394734 None:intergenic
AACGTGAATGCACTTCTTTG+TGG 0.447771 8.4:+33394564 MS.gene97956:CDS
GGCGATTTCCAGCCTTCTTA+CGG 0.449202 8.4:-33394658 None:intergenic
CTTTAAATTGAATAAAGGTC+AGG 0.450396 8.4:-33395532 None:intergenic
TCTGATGTGCCAAGGGCCTC+AGG 0.454537 8.4:+33395689 MS.gene97956:CDS
AACTTAACCATGGTTGTTGA+AGG 0.457176 8.4:-33394897 None:intergenic
CAGGTGCGATACGAGACAAT+TGG 0.467374 8.4:-33395513 None:intergenic
TCGCCAATGAGGATGAAGTT+GGG 0.469081 8.4:+33394676 MS.gene97956:CDS
GACAATTGGGAACACTTGGC+AGG 0.470535 8.4:-33395499 None:intergenic
CCCATCTTCCGTAAGAAGGC+TGG 0.470819 8.4:+33394650 MS.gene97956:CDS
GATGACAGAACCAAACGATT+GGG 0.471945 8.4:-33394820 None:intergenic
AACCCCAACTTCATCCTCAT+TGG 0.472522 8.4:-33394679 None:intergenic
TCTCTCTGGGACTAAACATC+TGG 0.472916 8.4:+33394866 MS.gene97956:CDS
TCAACAAATGACACCTCATC+TGG 0.479159 8.4:-33395569 None:intergenic
AGGTGCGATACGAGACAATT+GGG 0.480496 8.4:-33395512 None:intergenic
GTGTCCCATCTTCCGTAAGA+AGG 0.494377 8.4:+33394646 MS.gene97956:CDS
TAGTTTCTCTCCGCAAAGCT+TGG 0.495255 8.4:+33394614 MS.gene97956:CDS
ATCGCCAATGAGGATGAAGT+TGG 0.497075 8.4:+33394675 MS.gene97956:CDS
ATGTTAAGGTACTCAAGATC+AGG 0.497396 8.4:-33394720 None:intergenic
AGATACGCTTCCACGACATT+GGG 0.500275 8.4:-33394786 None:intergenic
AAGATACGCTTCCACGACAT+TGG 0.501628 8.4:-33394787 None:intergenic
CCAGCCTTCTTACGGAAGAT+GGG 0.510309 8.4:-33394650 None:intergenic
AGATTAAGAGATTCAATGAT+GGG 0.511116 8.4:-33394591 None:intergenic
GCAAATCAGCATGGATTATG+TGG 0.517789 8.4:+33394533 None:intergenic
TGACACATTCCTGAGGCCCT+TGG 0.520521 8.4:-33395698 None:intergenic
ACCCGGGAAGATTGAGCTCT+AGG 0.521976 8.4:-33395161 None:intergenic
GCCTAGAGCTCAATCTTCCC+GGG 0.523790 8.4:+33395160 MS.gene97956:CDS
GGATGACAGAACCAAACGAT+TGG 0.529615 8.4:-33394821 None:intergenic
ATTATTAGAACTTGAAGCAT+GGG 0.531928 8.4:-33395222 None:intergenic
AGTTGTACATGCTGAAAGCT+TGG 0.536926 8.4:-33394761 None:intergenic
AAGATTAAGAGATTCAATGA+TGG 0.540155 8.4:-33394592 None:intergenic
GCGCCAAGAAGGTTGTGTAA+AGG 0.540668 8.4:-33394843 None:intergenic
CGCCAATGAGGATGAAGTTG+GGG 0.543325 8.4:+33394677 MS.gene97956:CDS
CAGAGAATTTGAGTTAAACC+CGG 0.544044 8.4:-33395178 None:intergenic
ACGAGACAATTGGGAACACT+TGG 0.559054 8.4:-33395503 None:intergenic
TCCCAGAGAGAGCGCCAAGA+AGG 0.560841 8.4:-33394854 None:intergenic
AATTATTAGAACTTGAAGCA+TGG 0.571659 8.4:-33395223 None:intergenic
AAACATTATCTGATGTGCCA+AGG 0.577099 8.4:+33395681 MS.gene97956:CDS
AACATTATCTGATGTGCCAA+GGG 0.577206 8.4:+33395682 MS.gene97956:CDS
AGAGAATTTGAGTTAAACCC+GGG 0.586590 8.4:-33395177 None:intergenic
CATGGGACATTTCTGAAGCA+AGG 0.613005 8.4:-33395205 None:intergenic
GTTGAATCCTTCAACAACCA+TGG 0.615902 8.4:+33394890 MS.gene97956:CDS
TCTAATAATTCAGAATGACA+AGG 0.629883 8.4:+33395236 MS.gene97956:CDS
ACAATTGGGAACACTTGGCA+GGG 0.634349 8.4:-33395498 None:intergenic
ATGACAGAACCAAACGATTG+GGG 0.634767 8.4:-33394819 None:intergenic
ATTTCAAGGAATTCCAGATG+AGG 0.637246 8.4:+33395556 MS.gene97956:CDS
GTGTACAAACAGTGGCTGCT+TGG 0.639838 8.4:+33395093 MS.gene97956:intron
GTACAACTTACCCAATGTCG+TGG 0.648965 8.4:+33394776 MS.gene97956:CDS
AAGGCTGGAAATCGCCAATG+AGG 0.649702 8.4:+33394665 MS.gene97956:CDS
GATTAAGAGATTCAATGATG+GGG 0.667084 8.4:-33394590 None:intergenic
TAGGTCCAATGAACTATCAG+CGG 0.669674 8.4:-33395636 None:intergenic
TTTAAGTTGACACATTCCTG+AGG 0.695966 8.4:-33395705 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTCTTTAAATTGAATAA+AGG - chr8.4:33395540-33395559 None:intergenic 10.0%
!! ATTCAATTTAAAGAATTTCA+AGG + chr8.4:33395542-33395561 MS.gene97956:CDS 15.0%
!!! CAATATTCATTTTTTTGAAA+AGG - chr8.4:33395420-33395439 None:intergenic 15.0%
!! GAAATAAAAAAACTTAACCA+TGG - chr8.4:33394910-33394929 None:intergenic 20.0%
!! TATGAGTAAATCATGATTAT+TGG - chr8.4:33395028-33395047 None:intergenic 20.0%
!! TGTAATCTATTCATAAAAAC+TGG - chr8.4:33395313-33395332 None:intergenic 20.0%
!! TTAATCTTGCTAATATTAAG+TGG - chr8.4:33395369-33395388 None:intergenic 20.0%
! AAGATTAAGAGATTCAATGA+TGG - chr8.4:33394595-33394614 None:intergenic 25.0%
! AAGTTAAATTGACCAAATTC+AGG - chr8.4:33395276-33395295 None:intergenic 25.0%
! AATTATTAGAACTTGAAGCA+TGG - chr8.4:33395226-33395245 None:intergenic 25.0%
! AGATTAAGAGATTCAATGAT+GGG - chr8.4:33394594-33394613 None:intergenic 25.0%
! ATTATTAGAACTTGAAGCAT+GGG - chr8.4:33395225-33395244 None:intergenic 25.0%
! ATTCTTGAAAAAGTAGATCA+TGG - chr8.4:33395125-33395144 None:intergenic 25.0%
! CTTTAAATTGAATAAAGGTC+AGG - chr8.4:33395535-33395554 None:intergenic 25.0%
! TCTAATAATTCAGAATGACA+AGG + chr8.4:33395236-33395255 MS.gene97956:CDS 25.0%
! TTCTTGAAAAAGTAGATCAT+GGG - chr8.4:33395124-33395143 None:intergenic 25.0%
GATTAAGAGATTCAATGATG+GGG - chr8.4:33394593-33394612 None:intergenic 30.0%
TAAATTGACCAAATTCAGGT+TGG - chr8.4:33395272-33395291 None:intergenic 30.0%
TCTTGAAAAAGTAGATCATG+GGG - chr8.4:33395123-33395142 None:intergenic 30.0%
TGTTTAATCCAACCTGAATT+TGG + chr8.4:33395261-33395280 MS.gene97956:intron 30.0%
!! TACAGTTGCCAATTTTAGAA+TGG + chr8.4:33395471-33395490 MS.gene97956:CDS 30.0%
!!! ACAGTTGCCAATTTTAGAAT+GGG + chr8.4:33395472-33395491 MS.gene97956:CDS 30.0%
AAACATTATCTGATGTGCCA+AGG + chr8.4:33395681-33395700 MS.gene97956:CDS 35.0%
AACATTACCCGAATCACATT+TGG + chr8.4:33394738-33394757 MS.gene97956:CDS 35.0%
AACATTATCTGATGTGCCAA+GGG + chr8.4:33395682-33395701 MS.gene97956:CDS 35.0%
AACTTAACCATGGTTGTTGA+AGG - chr8.4:33394900-33394919 None:intergenic 35.0%
ACTGTACCTAAAAGAAACCA+AGG - chr8.4:33395457-33395476 None:intergenic 35.0%
ATTTCAAGGAATTCCAGATG+AGG + chr8.4:33395556-33395575 MS.gene97956:CDS 35.0%
GAATGTCGTACTTCTTCTTT+AGG - chr8.4:33395658-33395677 None:intergenic 35.0%
TTGCATCTTATTCAAGTCCT+TGG + chr8.4:33395437-33395456 MS.gene97956:intron 35.0%
TTGGCACATCAGATAATGTT+TGG - chr8.4:33395682-33395701 None:intergenic 35.0%
! AGAGAATTTGAGTTAAACCC+GGG - chr8.4:33395180-33395199 None:intergenic 35.0%
! ATGTGATTCGGGTAATGTTA+AGG - chr8.4:33394737-33394756 None:intergenic 35.0%
! ATGTTAAGGTACTCAAGATC+AGG - chr8.4:33394723-33394742 None:intergenic 35.0%
! CAGAGAATTTGAGTTAAACC+CGG - chr8.4:33395181-33395200 None:intergenic 35.0%
! TCAAGTCCTTGGTTTCTTTT+AGG + chr8.4:33395448-33395467 MS.gene97956:intron 35.0%
!! TTTAAGTTGACACATTCCTG+AGG - chr8.4:33395708-33395727 None:intergenic 35.0%
!!! GTTGAGCTTGTTTTGCAAAA+AGG + chr8.4:33395587-33395606 MS.gene97956:CDS 35.0%
AACGTGAATGCACTTCTTTG+TGG + chr8.4:33394564-33394583 MS.gene97956:CDS 40.0%
AAGCTTGGCCAAATGTGATT+CGG - chr8.4:33394749-33394768 None:intergenic 40.0%
AGTTGTACATGCTGAAAGCT+TGG - chr8.4:33394764-33394783 None:intergenic 40.0%
ATCCCTTTACACAACCTTCT+TGG + chr8.4:33394840-33394859 MS.gene97956:CDS 40.0%
ATGACAGAACCAAACGATTG+GGG - chr8.4:33394822-33394841 None:intergenic 40.0%
CAAACTTTGTCCAAGCTTTG+CGG - chr8.4:33394627-33394646 None:intergenic 40.0%
GATCAGGCGCATTTATTTCA+AGG - chr8.4:33394707-33394726 None:intergenic 40.0%
GATGACAGAACCAAACGATT+GGG - chr8.4:33394823-33394842 None:intergenic 40.0%
GATTACCGCTGATAGTTCAT+TGG + chr8.4:33395631-33395650 MS.gene97956:CDS 40.0%
GTTGAATCCTTCAACAACCA+TGG + chr8.4:33394890-33394909 MS.gene97956:CDS 40.0%
TAGGTCCAATGAACTATCAG+CGG - chr8.4:33395639-33395658 None:intergenic 40.0%
TCAACAAATGACACCTCATC+TGG - chr8.4:33395572-33395591 None:intergenic 40.0%
!! AGGGTAGCCCATTCTAAAAT+TGG - chr8.4:33395482-33395501 None:intergenic 40.0%
!! TTGATGACGTGTACAAACAG+TGG + chr8.4:33395085-33395104 MS.gene97956:intron 40.0%
AACCCCAACTTCATCCTCAT+TGG - chr8.4:33394682-33394701 None:intergenic 45.0%
AAGATACGCTTCCACGACAT+TGG - chr8.4:33394790-33394809 None:intergenic 45.0%
ACAATTGGGAACACTTGGCA+GGG - chr8.4:33395501-33395520 None:intergenic 45.0%
ACGAGACAATTGGGAACACT+TGG - chr8.4:33395506-33395525 None:intergenic 45.0%
AGATACGCTTCCACGACATT+GGG - chr8.4:33394789-33394808 None:intergenic 45.0%
AGCTTGGCCAAATGTGATTC+GGG - chr8.4:33394748-33394767 None:intergenic 45.0%
AGGTGCGATACGAGACAATT+GGG - chr8.4:33395515-33395534 None:intergenic 45.0%
ATCGCCAATGAGGATGAAGT+TGG + chr8.4:33394675-33394694 MS.gene97956:CDS 45.0%
CGCCAAGAAGGTTGTGTAAA+GGG - chr8.4:33394845-33394864 None:intergenic 45.0%
GATGTCTTTCCCCAATCGTT+TGG + chr8.4:33394810-33394829 MS.gene97956:CDS 45.0%
GGATGACAGAACCAAACGAT+TGG - chr8.4:33394824-33394843 None:intergenic 45.0%
GTACAACTTACCCAATGTCG+TGG + chr8.4:33394776-33394795 MS.gene97956:CDS 45.0%
TAGTTTCTCTCCGCAAAGCT+TGG + chr8.4:33394614-33394633 MS.gene97956:CDS 45.0%
TCGCCAATGAGGATGAAGTT+GGG + chr8.4:33394676-33394695 MS.gene97956:CDS 45.0%
!! CATGGGACATTTCTGAAGCA+AGG - chr8.4:33395208-33395227 None:intergenic 45.0%
!! TCTCTCTGGGACTAAACATC+TGG + chr8.4:33394866-33394885 MS.gene97956:CDS 45.0%
AAGGCTGGAAATCGCCAATG+AGG + chr8.4:33394665-33394684 MS.gene97956:CDS 50.0%
CAGGTGCGATACGAGACAAT+TGG - chr8.4:33395516-33395535 None:intergenic 50.0%
CCAGCCTTCTTACGGAAGAT+GGG - chr8.4:33394653-33394672 None:intergenic 50.0%
CGCCAATGAGGATGAAGTTG+GGG + chr8.4:33394677-33394696 MS.gene97956:CDS 50.0%
GACAATTGGGAACACTTGGC+AGG - chr8.4:33395502-33395521 None:intergenic 50.0%
GCGCCAAGAAGGTTGTGTAA+AGG - chr8.4:33394846-33394865 None:intergenic 50.0%
GGCGATTTCCAGCCTTCTTA+CGG - chr8.4:33394661-33394680 None:intergenic 50.0%
GTGTACAAACAGTGGCTGCT+TGG + chr8.4:33395093-33395112 MS.gene97956:intron 50.0%
GTGTCCCATCTTCCGTAAGA+AGG + chr8.4:33394646-33394665 MS.gene97956:CDS 50.0%
TCCAGCCTTCTTACGGAAGA+TGG - chr8.4:33394654-33394673 None:intergenic 50.0%
AACCTTCTTGGCGCTCTCTC+TGG + chr8.4:33394852-33394871 MS.gene97956:CDS 55.0%
ACCCGGGAAGATTGAGCTCT+AGG - chr8.4:33395164-33395183 None:intergenic 55.0%
ACCTTCTTGGCGCTCTCTCT+GGG + chr8.4:33394853-33394872 MS.gene97956:CDS 55.0%
CCCATCTTCCGTAAGAAGGC+TGG + chr8.4:33394650-33394669 MS.gene97956:CDS 55.0%
CGCCTAGAGCTCAATCTTCC+CGG + chr8.4:33395159-33395178 MS.gene97956:CDS 55.0%
GCCTAGAGCTCAATCTTCCC+GGG + chr8.4:33395160-33395179 MS.gene97956:CDS 55.0%
TGACACATTCCTGAGGCCCT+TGG - chr8.4:33395701-33395720 None:intergenic 55.0%
TCTGATGTGCCAAGGGCCTC+AGG + chr8.4:33395689-33395708 MS.gene97956:CDS 60.0%
!! TCCCAGAGAGAGCGCCAAGA+AGG - chr8.4:33394857-33394876 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 33394543 33395736 33394543 ID=MS.gene97956
chr8.4 mRNA 33394543 33395736 33394543 ID=MS.gene97956.t1;Parent=MS.gene97956
chr8.4 exon 33394543 33394911 33394543 ID=MS.gene97956.t1.exon1;Parent=MS.gene97956.t1
chr8.4 CDS 33394543 33394911 33394543 ID=cds.MS.gene97956.t1;Parent=MS.gene97956.t1
chr8.4 exon 33395105 33395257 33395105 ID=MS.gene97956.t1.exon2;Parent=MS.gene97956.t1
chr8.4 CDS 33395105 33395257 33395105 ID=cds.MS.gene97956.t1;Parent=MS.gene97956.t1
chr8.4 exon 33395470 33395736 33395470 ID=MS.gene97956.t1.exon3;Parent=MS.gene97956.t1
chr8.4 CDS 33395470 33395736 33395470 ID=cds.MS.gene97956.t1;Parent=MS.gene97956.t1
Gene Sequence

>MS.gene97956

ATGGATTATGTGGAAGTGTCTAACGTGAATGCACTTCTTTGTGGCTGCCCCATCATTGAATCTCTTAATCTTAGTTTCTCTCCGCAAAGCTTGGACAAAGTTTGTGTCCCATCTTCCGTAAGAAGGCTGGAAATCGCCAATGAGGATGAAGTTGGGGTTTACCTTGAAATAAATGCGCCTGATCTTGAGTACCTTAACATTACCCGAATCACATTTGGCCAAGCTTTCAGCATGTACAACTTACCCAATGTCGTGGAAGCGTATCTTGATGTCTTTCCCCAATCGTTTGGTTCTGTCATCCCTTTACACAACCTTCTTGGCGCTCTCTCTGGGACTAAACATCTGGTGTTGAATCCTTCAACAACCATGGTTAAGTTTTTTTATTTCTATTTTTTTTAAAAGTTATTTTATGTTCACATCATCATTTATACAGAGTATAAGTTAGTAAGCAACTGATAATAAACTAGTAACTAAGTATATCTCCAATAATCATGATTTACTCATATATAGATAGATATTGTTTTGTACTAATTAATTACTACTTGATGACGTGTACAAACAGTGGCTGCTTGGTGAACCCCATGATCTACTTTTTCAAGAATTTCAATATTTGCTTCGCCTAGAGCTCAATCTTCCCGGGTTTAACTCAAATTCTCTGCTCACCTTGCTTCAGAAATGTCCCATGCTTCAAGTTCTAATAATTCAGAATGACAAGGTTTGTTTAATCCAACCTGAATTTGGTCAATTTAACTTTGATGTGAAATTAACCAGTTTTTATGAATAGATTACACAAATTAGAAGTTGCATCTTATTGCACAGTATACCACTTAATATTAGCAAGATTAATTCATTACTATGAATATTGCATCTTATTCCTTTTCAAAAAAATGAATATTGCATCTTATTCAAGTCCTTGGTTTCTTTTAGGTACAGTTGCCAATTTTAGAATGGGCTACCCTGCCAAGTGTTCCCAATTGTCTCGTATCGCACCTGACCTTTATTCAATTTAAAGAATTTCAAGGAATTCCAGATGAGGTGTCATTTGTTGAGCTTGTTTTGCAAAAAGGACTCGTTTTAAAGACAATGATGATTACCGCTGATAGTTCATTGGACCTAAAGAAGAAGTACGACATTCTCCAAACATTATCTGATGTGCCAAGGGCCTCAGGAATGTGTCAACTTAAATTTGAATGA
Protein sequence

>MS.gene97956.t1

MDYVEVSNVNALLCGCPIIESLNLSFSPQSLDKVCVPSSVRRLEIANEDEVGVYLEINAPDLEYLNITRITFGQAFSMYNLPNVVEAYLDVFPQSFGSVIPLHNLLGALSGTKHLVLNPSTTMWLLGEPHDLLFQEFQYLLRLELNLPGFNSNSLLTLLQKCPMLQVLIIQNDKVQLPILEWATLPSVPNCLVSHLTFIQFKEFQGIPDEVSFVELVLQKGLVLKTMMITADSSLDLKKKYDILQTLSDVPRASGMCQLKFE