AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0905990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0905990 MtrunA17_Chr6g0468681 46.392 97 36 2 2 82 1 97 2.76e-19 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATAACACTCGACTGTTAA+AGG 0.173463 6_2:+4457873 None:intergenic
TCGCCATTGGAACGTGTTTC+CGG 0.199168 6_2:+4458251 None:intergenic
AATTTACCCATCATCATAAA+AGG 0.231721 6_2:+4458020 None:intergenic
TTAAAGGATGGTGGAAGGAG+GGG 0.305387 6_2:-4458298 Msa0905990:CDS
CTCACTGAGGAAAAGGTTAA+AGG 0.307407 6_2:-4458314 Msa0905990:CDS
AACAAATAAGAGATGGATCT+TGG 0.327273 6_2:-4458168 Msa0905990:CDS
GTCAAGAGAAAGCAAATTTA+CGG 0.332482 6_2:-4458079 Msa0905990:CDS
TCAATCAATCCAACCATAAC+TGG 0.335455 6_2:+4458353 None:intergenic
CATAACACTCGACTGTTAAA+GGG 0.389314 6_2:+4457874 None:intergenic
TCATATGTTTAATCCAGTTA+TGG 0.410645 6_2:-4458366 Msa0905990:CDS
TAAAGGATGGTGGAAGGAGG+GGG 0.411581 6_2:-4458297 Msa0905990:CDS
GCTTTCTCTTGACTACAATC+TGG 0.415041 6_2:+4458089 None:intergenic
GGTTAAAGGATGGTGGAAGG+AGG 0.417552 6_2:-4458300 Msa0905990:CDS
AGTAGGCTGGATAGATGTTC+CGG 0.432114 6_2:-4458270 Msa0905990:CDS
TTCAAGGCTCACTGAGGAAA+AGG 0.447645 6_2:-4458321 Msa0905990:CDS
GTTAAAGGATGGTGGAAGGA+GGG 0.464998 6_2:-4458299 Msa0905990:CDS
ATGATGGGTAAATTTAAAGA+TGG 0.496580 6_2:-4458011 Msa0905990:CDS
AAGAGATGGATCTTGGTTGA+AGG 0.508170 6_2:-4458161 Msa0905990:CDS
GATGTGAAATTGTCTTAATG+AGG 0.509961 6_2:+4458042 None:intergenic
ATGTTTAATCCAGTTATGGT+TGG 0.517438 6_2:-4458362 Msa0905990:CDS
TGATTGACACAAAACATTCA+AGG 0.536208 6_2:-4458337 Msa0905990:CDS
CTTCAGAAACGTATTGATGT+GGG 0.540400 6_2:-4458131 Msa0905990:CDS
GAAGTTTAACAAATAAGAGA+TGG 0.544075 6_2:-4458175 Msa0905990:CDS
GCTTCAGAAACGTATTGATG+TGG 0.565440 6_2:-4458132 Msa0905990:CDS
TGGCGAGAAAAGTGAGGGAA+TGG 0.574001 6_2:-4458234 Msa0905990:CDS
TGAATGCTCTCAGATTGTCC+TGG 0.580896 6_2:+4458398 None:intergenic
AGGAAAAGGTTAAAGGATGG+TGG 0.583886 6_2:-4458307 Msa0905990:CDS
ACGATGTCGGGATTAGAGAC+AGG 0.585282 6_2:-4457912 Msa0905990:CDS
AAAGGTTAAAGGATGGTGGA+AGG 0.586600 6_2:-4458303 Msa0905990:CDS
GTTCCGGAAACACGTTCCAA+TGG 0.594759 6_2:-4458254 Msa0905990:CDS
TCCAATGGCGAGAAAAGTGA+GGG 0.612722 6_2:-4458239 Msa0905990:CDS
TTCCAATGGCGAGAAAAGTG+AGG 0.630676 6_2:-4458240 Msa0905990:CDS
GTGAGGGAATGGTATTCGCG+TGG 0.647327 6_2:-4458223 Msa0905990:CDS
GTCACAGGAATTAATCAACC+AGG 0.651057 6_2:-4458416 Msa0905990:CDS
CTGAGGAAAAGGTTAAAGGA+TGG 0.658281 6_2:-4458310 Msa0905990:CDS
AAAACATTCAAGGCTCACTG+AGG 0.736310 6_2:-4458327 Msa0905990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGAACAAAATGTCAATTTT+AGG - chr6_2:4458320-4458339 Msa0905990:CDS 20.0%
!!! TGAACAAAATGTCAATTTTA+GGG - chr6_2:4458321-4458340 Msa0905990:CDS 20.0%
!!! TTAAAAAGCTCTGAAAATTT+AGG + chr6_2:4458358-4458377 None:intergenic 20.0%
! TCATATGTTTAATCCAGTTA+TGG - chr6_2:4457931-4457950 Msa0905990:CDS 25.0%
! GAAGTTTAACAAATAAGAGA+TGG - chr6_2:4458122-4458141 Msa0905990:CDS 25.0%
! AATTTACCCATCATCATAAA+AGG + chr6_2:4458280-4458299 None:intergenic 25.0%
!!! ATGATGGGTAAATTTAAAGA+TGG - chr6_2:4458286-4458305 Msa0905990:CDS 25.0%
!! AAATTGACATTTTGTTCATC+TGG + chr6_2:4458319-4458338 None:intergenic 25.0%
ATGTTTAATCCAGTTATGGT+TGG - chr6_2:4457935-4457954 Msa0905990:CDS 30.0%
TGATTGACACAAAACATTCA+AGG - chr6_2:4457960-4457979 Msa0905990:CDS 30.0%
AACAAATAAGAGATGGATCT+TGG - chr6_2:4458129-4458148 Msa0905990:CDS 30.0%
! TATTGATGTGGGTTTTCTTT+TGG - chr6_2:4458177-4458196 Msa0905990:CDS 30.0%
GTCAAGAGAAAGCAAATTTA+CGG - chr6_2:4458218-4458237 Msa0905990:CDS 30.0%
GATGTGAAATTGTCTTAATG+AGG + chr6_2:4458258-4458277 None:intergenic 30.0%
! TTCACATCCTTTTATGATGA+TGG - chr6_2:4458270-4458289 Msa0905990:CDS 30.0%
! TCACATCCTTTTATGATGAT+GGG - chr6_2:4458271-4458290 Msa0905990:CDS 30.0%
!!! TTTTAAGTTTTCGACGATGT+CGG - chr6_2:4458372-4458391 Msa0905990:CDS 30.0%
!!! ATTTTAGGTTGGAATGTCAC+AGG - chr6_2:4457866-4457885 Msa0905990:CDS 35.0%
TCAATCAATCCAACCATAAC+TGG + chr6_2:4457947-4457966 None:intergenic 35.0%
! CTTCAGAAACGTATTGATGT+GGG - chr6_2:4458166-4458185 Msa0905990:CDS 35.0%
! TTGACATTTTGTTCATCTGG+TGG + chr6_2:4458316-4458335 None:intergenic 35.0%
!!! GTCAATTTTAGGGTTTTTGC+AGG - chr6_2:4458331-4458350 Msa0905990:CDS 35.0%
!! TTTAAGTTTTCGACGATGTC+GGG - chr6_2:4458373-4458392 Msa0905990:CDS 35.0%
CATAACACTCGACTGTTAAA+GGG + chr6_2:4458426-4458445 None:intergenic 35.0%
TCATAACACTCGACTGTTAA+AGG + chr6_2:4458427-4458446 None:intergenic 35.0%
GTCACAGGAATTAATCAACC+AGG - chr6_2:4457881-4457900 Msa0905990:CDS 40.0%
AAAACATTCAAGGCTCACTG+AGG - chr6_2:4457970-4457989 Msa0905990:CDS 40.0%
CTCACTGAGGAAAAGGTTAA+AGG - chr6_2:4457983-4458002 Msa0905990:CDS 40.0%
CTGAGGAAAAGGTTAAAGGA+TGG - chr6_2:4457987-4458006 Msa0905990:CDS 40.0%
AGGAAAAGGTTAAAGGATGG+TGG - chr6_2:4457990-4458009 Msa0905990:CDS 40.0%
!! AAAGGTTAAAGGATGGTGGA+AGG - chr6_2:4457994-4458013 Msa0905990:CDS 40.0%
AAGAGATGGATCTTGGTTGA+AGG - chr6_2:4458136-4458155 Msa0905990:CDS 40.0%
! GCTTCAGAAACGTATTGATG+TGG - chr6_2:4458165-4458184 Msa0905990:CDS 40.0%
GCTTTCTCTTGACTACAATC+TGG + chr6_2:4458211-4458230 None:intergenic 40.0%
TGAATGCTCTCAGATTGTCC+TGG + chr6_2:4457902-4457921 None:intergenic 45.0%
TTCAAGGCTCACTGAGGAAA+AGG - chr6_2:4457976-4457995 Msa0905990:CDS 45.0%
!! GTTAAAGGATGGTGGAAGGA+GGG - chr6_2:4457998-4458017 Msa0905990:CDS 45.0%
!! TTAAAGGATGGTGGAAGGAG+GGG - chr6_2:4457999-4458018 Msa0905990:CDS 45.0%
AGTAGGCTGGATAGATGTTC+CGG - chr6_2:4458027-4458046 Msa0905990:CDS 45.0%
TTCCAATGGCGAGAAAAGTG+AGG - chr6_2:4458057-4458076 Msa0905990:CDS 45.0%
TCCAATGGCGAGAAAAGTGA+GGG - chr6_2:4458058-4458077 Msa0905990:CDS 45.0%
!! GGTTAAAGGATGGTGGAAGG+AGG - chr6_2:4457997-4458016 Msa0905990:CDS 50.0%
!! TAAAGGATGGTGGAAGGAGG+GGG - chr6_2:4458000-4458019 Msa0905990:CDS 50.0%
GTTCCGGAAACACGTTCCAA+TGG - chr6_2:4458043-4458062 Msa0905990:CDS 50.0%
TCGCCATTGGAACGTGTTTC+CGG + chr6_2:4458049-4458068 None:intergenic 50.0%
!! TCCCTCACTTTTCTCGCCAT+TGG + chr6_2:4458062-4458081 None:intergenic 50.0%
TGGCGAGAAAAGTGAGGGAA+TGG - chr6_2:4458063-4458082 Msa0905990:CDS 50.0%
ACGATGTCGGGATTAGAGAC+AGG - chr6_2:4458385-4458404 Msa0905990:CDS 50.0%
TGGAAGGAGGGGGACAAAGT+AGG - chr6_2:4458010-4458029 Msa0905990:CDS 55.0%
GTGAGGGAATGGTATTCGCG+TGG - chr6_2:4458074-4458093 Msa0905990:CDS 55.0%
AGGAGGGGGACAAAGTAGGC+TGG - chr6_2:4458014-4458033 Msa0905990:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 4457857 4458462 4457857 ID=Msa0905990;Name=Msa0905990
chr6_2 mRNA 4457857 4458462 4457857 ID=Msa0905990-mRNA-1;Parent=Msa0905990;Name=Msa0905990-mRNA-1;_AED=0.14;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|201
chr6_2 exon 4457857 4458462 4457857 ID=Msa0905990-mRNA-1:exon:1294;Parent=Msa0905990-mRNA-1
chr6_2 CDS 4457857 4458462 4457857 ID=Msa0905990-mRNA-1:cds;Parent=Msa0905990-mRNA-1
Gene Sequence

>Msa0905990

ATGATGAATATTTTAGGTTGGAATGTCACAGGAATTAATCAACCAGGACAATCTGAGAGCATTCATCAGCTTGTTCATATGTTTAATCCAGTTATGGTTGGATTGATTGACACAAAACATTCAAGGCTCACTGAGGAAAAGGTTAAAGGATGGTGGAAGGAGGGGGACAAAGTAGGCTGGATAGATGTTCCGGAAACACGTTCCAATGGCGAGAAAAGTGAGGGAATGGTATTCGCGTGGAATGAACGTTGTTTTATGAAATCAAGAAGTTTAACAAATAAGAGATGGATCTTGGTTGAAGGAAAGCTGCTTCAGAAACGTATTGATGTGGGTTTTCTTTTGGTTTTTGAACCAGATTGTAGTCAAGAGAAAGCAAATTTACGGAATGATTTGACGAACCTCATTAAGACAATTTCACATCCTTTTATGATGATGGGTAAATTTAAAGATGGTTCGCCACCAGATGAACAAAATGTCAATTTTAGGGTTTTTGCAGGTCCTAAATTTTCAGAGCTTTTTAAGTTTTCGACGATGTCGGGATTAGAGACAGGTCTCTCAGATCACTGCCCTTTAACAGTCGAGTGTTATGAAAAAGATAATATTTGA

Protein sequence

>Msa0905990

MMNILGWNVTGINQPGQSESIHQLVHMFNPVMVGLIDTKHSRLTEEKVKGWWKEGDKVGWIDVPETRSNGEKSEGMVFAWNERCFMKSRSLTNKRWILVEGKLLQKRIDVGFLLVFEPDCSQEKANLRNDLTNLIKTISHPFMMMGKFKDGSPPDEQNVNFRVFAGPKFSELFKFSTMSGLETGLSDHCPLTVECYEKDNI*