AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39482

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39482.t1 MTR_4g088615 97.030 303 9 0 1 303 1 303 0.0 600
MS.gene39482.t1 MTR_4g088615 96.364 220 8 0 1 220 1 220 5.81e-155 433
MS.gene39482.t1 MTR_8g105340 32.727 165 94 4 147 303 82 237 3.14e-16 77.4
MS.gene39482.t1 MTR_8g105340 32.727 165 94 4 147 303 91 246 3.53e-16 77.4
MS.gene39482.t1 MTR_1g032450 32.727 165 94 4 147 303 89 244 4.40e-16 77.0
MS.gene39482.t1 MTR_8g093680 30.065 153 107 0 143 295 341 493 1.15e-15 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39482.t1 AT2G33800 71.321 265 68 3 44 303 42 303 2.41e-130 373
MS.gene39482.t1 AT1G64880 33.553 152 101 0 144 295 355 506 1.50e-18 86.3
MS.gene39482.t1 AT2G41840 31.515 165 96 3 147 303 96 251 5.54e-16 77.0
MS.gene39482.t1 AT1G58380 31.515 165 96 3 147 303 95 250 6.86e-16 76.6
MS.gene39482.t1 AT1G58684 31.515 165 96 3 147 303 95 250 8.42e-16 76.6
MS.gene39482.t1 AT1G58983 31.515 165 96 3 147 303 95 250 8.42e-16 76.6
MS.gene39482.t1 AT1G59359 31.515 165 96 3 147 303 95 250 8.42e-16 76.6
MS.gene39482.t1 AT3G57490 30.303 165 98 3 147 303 87 242 1.11e-15 75.9

Find 110 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGAGCTGATGGAGATTA+TGG 0.212582 4.2:-22459210 MS.gene39482:intron
GAGGGAAACAAATGAGGTTT+AGG 0.287989 4.2:-22460892 MS.gene39482:CDS
GAATGTGAGTTTGTGGGTTG+TGG 0.290893 4.2:+22461291 None:intergenic
TTGAAGGGAAGGTCTTCTTC+TGG 0.304660 4.2:+22461181 None:intergenic
TTCGACTGGACCGTCGTCTA+TGG 0.308957 4.2:+22461120 None:intergenic
ATGAAGCCTTCCGGGATTTC+CGG 0.310489 4.2:+22461154 None:intergenic
GGAGAGAGAGAATGGAGTTT+TGG 0.320230 4.2:+22461261 None:intergenic
CGGGATTTCCGGTGGGTTGA+AGG 0.323166 4.2:+22461165 None:intergenic
AAGGATACCGGGTTCGGTTC+GGG 0.333237 4.2:-22460992 MS.gene39482:CDS
GAAGGATACCGGGTTCGGTT+CGG 0.346246 4.2:-22460993 MS.gene39482:CDS
GGATCCCTATGGAAGAATTA+TGG 0.351460 4.2:-22458984 MS.gene39482:CDS
TGAAGGGAAGGTCTTCTTCT+GGG 0.352947 4.2:+22461182 None:intergenic
AATGTGAGTTTGTGGGTTGT+GGG 0.361016 4.2:+22461292 None:intergenic
CCTGGTACCGGAGTTATTGC+AGG 0.364105 4.2:-22459156 MS.gene39482:CDS
GAGAGAGTGGAGAGAGAGAA+TGG 0.370271 4.2:+22461253 None:intergenic
AGGGTTACGAAGGTTGTTAA+AGG 0.376413 4.2:-22460914 MS.gene39482:CDS
GTGGTGGAATGAAGCCTTCC+GGG 0.382928 4.2:+22461146 None:intergenic
GCCGGTGTTGAGAATGCTTT+GGG 0.389280 4.2:-22459105 MS.gene39482:CDS
TTTAGGGCTATTGTTGTTGT+TGG 0.390533 4.2:-22460875 MS.gene39482:CDS
CACTGCAGCTACAGTTGCTC+TGG 0.409585 4.2:+22459047 None:intergenic
GGTGGTGGAATGAAGCCTTC+CGG 0.420960 4.2:+22461145 None:intergenic
GGAAAAGATAAGAGATGGGT+TGG 0.430497 4.2:-22460960 MS.gene39482:CDS
AGGGAAACAAATGAGGTTTA+GGG 0.444508 4.2:-22460891 MS.gene39482:CDS
AAGAGTTGTTCAAGTGAGGA+GGG 0.451100 4.2:-22460933 MS.gene39482:CDS
TGAGAGGGAATGTGAGTTTG+TGG 0.452351 4.2:+22461284 None:intergenic
TGTGAGAATTGTTCTTGAAA+TGG 0.455018 4.2:-22459127 MS.gene39482:CDS
ATGCTTAGACCCGCTTCTCC+TGG 0.460234 4.2:-22459174 MS.gene39482:CDS
ATTTCATCTTCGTCTTCGAC+TGG 0.464676 4.2:+22461106 None:intergenic
GGCCGGTGTTGAGAATGCTT+TGG 0.464816 4.2:-22459106 MS.gene39482:CDS
AAGAAGGAAAAGATAAGAGA+TGG 0.465607 4.2:-22460965 MS.gene39482:CDS
TTTCCGGTGGGTTGAAGGGA+AGG 0.470346 4.2:+22461170 None:intergenic
TACAGTGGTGTTAGTGTGCT+TGG 0.475022 4.2:-22461049 MS.gene39482:CDS
TCTCTCCACTCTCTCCTTCG+CGG 0.475222 4.2:-22461245 MS.gene39482:CDS
GACGAGTTGGTGTTGGTGTT+GGG 0.477749 4.2:-22460841 MS.gene39482:CDS
GCGAGGAAGGATACCGGGTT+CGG 0.479438 4.2:-22460998 MS.gene39482:CDS
AGAGAATTGAGTGCTGAAGA+AGG 0.483087 4.2:+22461367 None:intergenic
GTTCGGTTCGGGTGCGAAGA+AGG 0.486778 4.2:-22460981 MS.gene39482:CDS
GAGAGGGAATGTGAGTTTGT+GGG 0.488658 4.2:+22461285 None:intergenic
GGACGAGTTGGTGTTGGTGT+TGG 0.490278 4.2:-22460842 MS.gene39482:CDS
GAAACAAGAGGAGGAAGAAG+AGG 0.504513 4.2:+22461324 None:intergenic
AAAGAGTTGTTCAAGTGAGG+AGG 0.507039 4.2:-22460934 MS.gene39482:CDS
GGGAAGGTCTTCTTCTGGGA+TGG 0.507275 4.2:+22461186 None:intergenic
CTTCAACCCACCGGAAATCC+CGG 0.509862 4.2:-22461164 MS.gene39482:CDS
GCCGCATACGAAGAGCTATA+CGG 0.511899 4.2:-22461079 MS.gene39482:CDS
AAGACGGCGAGGAAGGATAC+CGG 0.519140 4.2:-22461004 MS.gene39482:CDS
TACTTCCATAATTCTTCCAT+AGG 0.523427 4.2:+22458979 None:intergenic
AGAAGGAAAAGATAAGAGAT+GGG 0.524914 4.2:-22460964 MS.gene39482:CDS
TTAAAGGAGGGAAACAAATG+AGG 0.524916 4.2:-22460898 MS.gene39482:CDS
AAGAAAGAGTTGTTCAAGTG+AGG 0.526597 4.2:-22460937 MS.gene39482:CDS
GTTACGAAGGTTGTTAAAGG+AGG 0.526853 4.2:-22460911 MS.gene39482:CDS
CTTCGCACCCGAACCGAACC+CGG 0.526945 4.2:+22460985 None:intergenic
AAGCCTTCCGGGATTTCCGG+TGG 0.529062 4.2:+22461157 None:intergenic
ATGTGAGTTTGTGGGTTGTG+GGG 0.529517 4.2:+22461293 None:intergenic
GGGATTTCCGGTGGGTTGAA+GGG 0.530494 4.2:+22461166 None:intergenic
GATGGTGTTGTCGAATTCAT+CGG 0.536992 4.2:+22461204 None:intergenic
AAAAGTCAAGTACTTGGTCA+TGG 0.539176 4.2:+22460748 None:intergenic
AGAAATCTGCTATTAATGCT+AGG 0.542732 4.2:-22460790 MS.gene39482:CDS
CCGTCGTCTATGGAAGGTGG+TGG 0.551471 4.2:+22461130 None:intergenic
AATGCTTTGGGGAAACAACT+AGG 0.554037 4.2:-22459093 MS.gene39482:CDS
TGAGTTTGTGGGTTGTGGGG+AGG 0.554542 4.2:+22461296 None:intergenic
ATGAGGAAAAGTCAAGTACT+TGG 0.555126 4.2:+22460742 None:intergenic
GTTGTCGAATTCATCGGAGG+TGG 0.557558 4.2:+22461210 None:intergenic
GTTAGAGAAAGTTACCTATG+AGG 0.558410 4.2:+22460725 None:intergenic
GATGGATAAGACGGCGAGGA+AGG 0.559767 4.2:-22461011 MS.gene39482:CDS
TGGAAATGATATAAATGTGA+TGG 0.562907 4.2:-22461029 MS.gene39482:CDS
CCTGCAATAACTCCGGTACC+AGG 0.564590 4.2:+22459156 None:intergenic
TGAGGAGCGTGGGATCCCTA+TGG 0.565454 4.2:-22458995 MS.gene39482:CDS
ACTTCCATAATTCTTCCATA+GGG 0.566653 4.2:+22458980 None:intergenic
TGGAGATTATGGAGCGTCAA+AGG 0.569341 4.2:-22459199 MS.gene39482:CDS
ACTCCGGTACCAGGAGAAGC+GGG 0.569424 4.2:+22459165 None:intergenic
AGTGAGGTCGCTGAGGAGCG+TGG 0.573201 4.2:-22459006 MS.gene39482:CDS
AGACCCGCTTCTCCTGGTAC+CGG 0.574190 4.2:-22459168 MS.gene39482:CDS
GGTACCGGAGTTATTGCAGG+TGG 0.578070 4.2:-22459153 MS.gene39482:CDS
AAAGTCAAGTACTTGGTCAT+GGG 0.581671 4.2:+22460749 None:intergenic
AACTCCGGTACCAGGAGAAG+CGG 0.582114 4.2:+22459164 None:intergenic
AGAATTGTTCTTGAAATGGC+CGG 0.583079 4.2:-22459123 MS.gene39482:CDS
TTACGAAGGTTGTTAAAGGA+GGG 0.584504 4.2:-22460910 MS.gene39482:CDS
GTGAGGTCGCTGAGGAGCGT+GGG 0.589602 4.2:-22459005 MS.gene39482:CDS
GGGGAGGAGAGAGAAACAAG+AGG 0.595007 4.2:+22461312 None:intergenic
CCCCAAAGCATTCTCAACAC+CGG 0.599175 4.2:+22459104 None:intergenic
AGCCTTCCGGGATTTCCGGT+GGG 0.602553 4.2:+22461158 None:intergenic
AGACGGCGAGGAAGGATACC+GGG 0.603653 4.2:-22461003 MS.gene39482:CDS
GAGGAGAGAGAAACAAGAGG+AGG 0.603782 4.2:+22461315 None:intergenic
ACTGGACCGTCGTCTATGGA+AGG 0.605529 4.2:+22461124 None:intergenic
TATAAATGTGATGGATAAGA+CGG 0.605582 4.2:-22461020 MS.gene39482:CDS
GGGAATTGCTTTGACCGCGA+AGG 0.607546 4.2:+22461231 None:intergenic
AGACCTTCCCTTCAACCCAC+CGG 0.615775 4.2:-22461173 MS.gene39482:CDS
AAGAGGAGGAAGAAGAGGAA+CGG 0.616236 4.2:+22461329 None:intergenic
GCAGTACAGTGAGGTCGCTG+AGG 0.616630 4.2:-22459013 MS.gene39482:CDS
TTGTCGAATTCATCGGAGGT+GGG 0.616706 4.2:+22461211 None:intergenic
GTTGTTGTTGGTGATAAGCA+AGG 0.618084 4.2:-22460863 MS.gene39482:CDS
TTTGACCGCGAAGGAGAGAG+TGG 0.633669 4.2:+22461240 None:intergenic
AAGCAAGGACGAGTTGGTGT+TGG 0.635148 4.2:-22460848 MS.gene39482:CDS
GGTGATAAGCAAGGACGAGT+TGG 0.638537 4.2:-22460854 MS.gene39482:CDS
TGTTGGTGTTGGGAAAGCTA+AGG 0.639378 4.2:-22460831 MS.gene39482:CDS
ACCGTATAGCTCTTCGTATG+CGG 0.640746 4.2:+22461078 None:intergenic
AGACCCACCTGCAATAACTC+CGG 0.641456 4.2:+22459149 None:intergenic
TCAAGTGAGGAGGGTTACGA+AGG 0.645808 4.2:-22460924 MS.gene39482:CDS
AACCCACCGGAAATCCCGGA+AGG 0.651503 4.2:-22461160 MS.gene39482:CDS
GGACCGTCGTCTATGGAAGG+TGG 0.656325 4.2:+22461127 None:intergenic
AATCTGCTATTAATGCTAGG+AGG 0.657188 4.2:-22460787 MS.gene39482:CDS
CTATACGGTGTGGCGTACAG+TGG 0.669321 4.2:-22461064 MS.gene39482:CDS
GTACCGGAGTTATTGCAGGT+GGG 0.671941 4.2:-22459152 MS.gene39482:CDS
GAAAATGAAGCAGTACAGTG+AGG 0.690707 4.2:-22459022 MS.gene39482:CDS
GTATAGCTCTTCGTATGCGG+CGG 0.690747 4.2:+22461081 None:intergenic
GGTGTTGTCGAATTCATCGG+AGG 0.692934 4.2:+22461207 None:intergenic
ATGTGATGGATAAGACGGCG+AGG 0.709872 4.2:-22461015 MS.gene39482:CDS
CCACCACCTTCCATAGACGA+CGG 0.718416 4.2:-22461130 MS.gene39482:CDS
ATACGAAGAGCTATACGGTG+TGG 0.743299 4.2:-22461074 MS.gene39482:CDS
CCGGTGTTGAGAATGCTTTG+GGG 0.778015 4.2:-22459104 MS.gene39482:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTCATCAATCTTAATTT+TGG - chr4.2:22459661-22459680 MS.gene39482:intron 15.0%
!! AAACTTATGGATGAAAAAAT+TGG - chr4.2:22459911-22459930 MS.gene39482:intron 20.0%
!! AACTTATGGATGAAAAAATT+GGG - chr4.2:22459912-22459931 MS.gene39482:intron 20.0%
!! ATTATGAATAATGCAATTCA+AGG + chr4.2:22460161-22460180 None:intergenic 20.0%
!! ATTTGGATATTTGTGTATAT+AGG - chr4.2:22460201-22460220 MS.gene39482:intron 20.0%
!! TAAATTTCCTTAACTCAAAT+AGG - chr4.2:22460955-22460974 MS.gene39482:CDS 20.0%
!! TATATACGACATAAAATTAG+TGG + chr4.2:22461120-22461139 None:intergenic 20.0%
! AAATATCCACCATATAAGAA+GGG + chr4.2:22460535-22460554 None:intergenic 25.0%
! ACAATGTCATATTAATATGG+AGG + chr4.2:22460564-22460583 None:intergenic 25.0%
! TATCATTATCACTATGTTTC+TGG - chr4.2:22460487-22460506 MS.gene39482:intron 25.0%
! TCCATATTAATATGACATTG+TGG - chr4.2:22460563-22460582 MS.gene39482:intron 25.0%
! TGAGAAACGAATATGAAATA+TGG - chr4.2:22460661-22460680 MS.gene39482:intron 25.0%
! TGAGTTAAGGAAATTTAACA+GGG + chr4.2:22460952-22460971 None:intergenic 25.0%
! TGGAAATGATATAAATGTGA+TGG - chr4.2:22459328-22459347 MS.gene39482:intron 25.0%
! TTGAGTTAAGGAAATTTAAC+AGG + chr4.2:22460953-22460972 None:intergenic 25.0%
! TTGTGTATATAGGAGTTATT+GGG - chr4.2:22460211-22460230 MS.gene39482:intron 25.0%
! TTTGTGTATATAGGAGTTAT+TGG - chr4.2:22460210-22460229 MS.gene39482:intron 25.0%
!! AATATAATGAAGCATTCTTG+TGG - chr4.2:22460266-22460285 MS.gene39482:intron 25.0%
!! CTAATTTTTCTTTGACATGA+TGG - chr4.2:22460350-22460369 MS.gene39482:intron 25.0%
!!! AGATGGAACTTTTATTTCAA+AGG - chr4.2:22461008-22461027 MS.gene39482:CDS 25.0%
!!! ATTTTTACATAGAGCATTAC+AGG + chr4.2:22460642-22460661 None:intergenic 25.0%
!!! GGTTTTGAATTTGAAACTTA+TGG - chr4.2:22459898-22459917 MS.gene39482:intron 25.0%
!!! TATAAATGTGATGGATAAGA+CGG - chr4.2:22459337-22459356 MS.gene39482:intron 25.0%
AAGAAGGAAAAGATAAGAGA+TGG - chr4.2:22459392-22459411 MS.gene39482:intron 30.0%
AATTCTAGCATCTTAGAACA+CGG + chr4.2:22461065-22461084 None:intergenic 30.0%
ACAATGTTAAGAACACAAGA+AGG + chr4.2:22460117-22460136 None:intergenic 30.0%
ACTTCCATAATTCTTCCATA+GGG + chr4.2:22461380-22461399 None:intergenic 30.0%
AGAAATCTGCTATTAATGCT+AGG - chr4.2:22459567-22459586 MS.gene39482:intron 30.0%
AGAAGGAAAAGATAAGAGAT+GGG - chr4.2:22459393-22459412 MS.gene39482:intron 30.0%
ATGAATGCCTAAGTAACTTA+TGG - chr4.2:22460900-22460919 MS.gene39482:CDS 30.0%
ATTCCTTCTTCTATTCTCAA+TGG - chr4.2:22460039-22460058 MS.gene39482:intron 30.0%
CAAATATCCACCATATAAGA+AGG + chr4.2:22460536-22460555 None:intergenic 30.0%
CATTGTATTAGAGCTTCATT+TGG - chr4.2:22460184-22460203 MS.gene39482:intron 30.0%
CTAAACTAATCTCATTTACG+TGG + chr4.2:22459795-22459814 None:intergenic 30.0%
GCCACAATGTCATATTAATA+TGG + chr4.2:22460567-22460586 None:intergenic 30.0%
TAAGTGACCATAAGTTACTT+AGG + chr4.2:22460910-22460929 None:intergenic 30.0%
TACTTCCATAATTCTTCCAT+AGG + chr4.2:22461381-22461400 None:intergenic 30.0%
TGTGAGAATTGTTCTTGAAA+TGG - chr4.2:22461230-22461249 MS.gene39482:CDS 30.0%
TTAACAGGGAACATCTTAAT+AGG + chr4.2:22460938-22460957 None:intergenic 30.0%
TTATGGATGAAAAAATTGGG+TGG - chr4.2:22459915-22459934 MS.gene39482:intron 30.0%
! AGATTAGTCATTAGCATAGT+TGG - chr4.2:22459958-22459977 MS.gene39482:intron 30.0%
! GTTTTCCTGAATTTCAAATG+AGG + chr4.2:22460239-22460258 None:intergenic 30.0%
! TTAGCATCGTAAGATTTTAC+TGG - chr4.2:22460699-22460718 MS.gene39482:intron 30.0%
! TTCAGTTCCTATTTGAGTTA+AGG + chr4.2:22460965-22460984 None:intergenic 30.0%
!! TTATATGGTGGATATTTGTC+TGG - chr4.2:22460538-22460557 MS.gene39482:intron 30.0%
AAAAGTCAAGTACTTGGTCA+TGG + chr4.2:22459612-22459631 None:intergenic 35.0%
AAAGTCAAGTACTTGGTCAT+GGG + chr4.2:22459611-22459630 None:intergenic 35.0%
AACTCGATAGAAACAAGCAA+AGG + chr4.2:22460085-22460104 None:intergenic 35.0%
AAGAAAGAGTTGTTCAAGTG+AGG - chr4.2:22459420-22459439 MS.gene39482:intron 35.0%
AATCTGCTATTAATGCTAGG+AGG - chr4.2:22459570-22459589 MS.gene39482:intron 35.0%
ACATATGGCATGATAGTCAA+AGG - chr4.2:22459992-22460011 MS.gene39482:intron 35.0%
ACCATCAAATGCTCAAATGA+AGG + chr4.2:22460725-22460744 None:intergenic 35.0%
ACTTTGTGTACTGAACATTG+AGG - chr4.2:22460313-22460332 MS.gene39482:intron 35.0%
AGAATTGTTCTTGAAATGGC+CGG - chr4.2:22461234-22461253 MS.gene39482:CDS 35.0%
AGATGCGTTATATTCAGCTT+TGG + chr4.2:22460763-22460782 None:intergenic 35.0%
AGGGAAACAAATGAGGTTTA+GGG - chr4.2:22459466-22459485 MS.gene39482:intron 35.0%
ATGAGGAAAAGTCAAGTACT+TGG + chr4.2:22459618-22459637 None:intergenic 35.0%
ATTCTAGCATCTTAGAACAC+GGG + chr4.2:22461064-22461083 None:intergenic 35.0%
CAAAGCAGATTCCACTATAT+AGG + chr4.2:22460589-22460608 None:intergenic 35.0%
CAATTTGACAATGTGAGACA+CGG + chr4.2:22460400-22460419 None:intergenic 35.0%
CATGATAGTCAAAGGTAAAG+GGG - chr4.2:22460000-22460019 MS.gene39482:intron 35.0%
CTTATTCCACTAAGTGAGTT+CGG + chr4.2:22459749-22459768 None:intergenic 35.0%
GAATATAACGCATCTGATTG+AGG - chr4.2:22460768-22460787 MS.gene39482:CDS 35.0%
GATAGAAACAAGCAAAGGTA+AGG + chr4.2:22460080-22460099 None:intergenic 35.0%
GCATGATAGTCAAAGGTAAA+GGG - chr4.2:22459999-22460018 MS.gene39482:intron 35.0%
GTTAGAGAAAGTTACCTATG+AGG + chr4.2:22459635-22459654 None:intergenic 35.0%
GTTCCATTGAGAATAGAAGA+AGG + chr4.2:22460045-22460064 None:intergenic 35.0%
TGCGATAAGCATTCACAAAT+GGG + chr4.2:22460861-22460880 None:intergenic 35.0%
TTAAAGGAGGGAAACAAATG+AGG - chr4.2:22459459-22459478 MS.gene39482:intron 35.0%
TTACGAAGGTTGTTAAAGGA+GGG - chr4.2:22459447-22459466 MS.gene39482:intron 35.0%
TTGGCAAATGCTTTGACATA+TGG - chr4.2:22459977-22459996 MS.gene39482:intron 35.0%
! AGTACTTGACTTTTCCTCAT+AGG - chr4.2:22459618-22459637 MS.gene39482:intron 35.0%
! ATATAGTGGAATCTGCTTTG+TGG - chr4.2:22460589-22460608 MS.gene39482:intron 35.0%
! TAATGTTTTTCCAGAGCTGA+TGG - chr4.2:22461138-22461157 MS.gene39482:CDS 35.0%
! TTTAGGGCTATTGTTGTTGT+TGG - chr4.2:22459482-22459501 MS.gene39482:intron 35.0%
!! ATGAGAAACTTGTTGACCAA+TGG - chr4.2:22459836-22459855 MS.gene39482:intron 35.0%
!! TGAGAAACTTGTTGACCAAT+GGG - chr4.2:22459837-22459856 MS.gene39482:intron 35.0%
AAAGAGTTGTTCAAGTGAGG+AGG - chr4.2:22459423-22459442 MS.gene39482:intron 40.0%
AAAGGTAAAGGGGAAATCCT+TGG - chr4.2:22460010-22460029 MS.gene39482:intron 40.0%
AAGAGTTGTTCAAGTGAGGA+GGG - chr4.2:22459424-22459443 MS.gene39482:intron 40.0%
AATGCTTATCGCAGCTGAAA+GGG - chr4.2:22460868-22460887 MS.gene39482:CDS 40.0%
ACATTGAGGTAGAACAGACT+CGG - chr4.2:22460327-22460346 MS.gene39482:intron 40.0%
ACTAAGTGAGTTCGGTTACA+TGG + chr4.2:22459741-22459760 None:intergenic 40.0%
AGGGTTACGAAGGTTGTTAA+AGG - chr4.2:22459443-22459462 MS.gene39482:intron 40.0%
ATGTAACCGAACTCACTTAG+TGG - chr4.2:22459740-22459759 MS.gene39482:intron 40.0%
ATTTCATCTTCGTCTTCGAC+TGG + chr4.2:22459254-22459273 None:intergenic 40.0%
CATCTGATTGAGGTTGTAGT+TGG - chr4.2:22460778-22460797 MS.gene39482:CDS 40.0%
CGAACTCACTTAGTGGAATA+AGG - chr4.2:22459747-22459766 MS.gene39482:intron 40.0%
CTGCGATAAGCATTCACAAA+TGG + chr4.2:22460862-22460881 None:intergenic 40.0%
GAAAATGAAGCAGTACAGTG+AGG - chr4.2:22461335-22461354 MS.gene39482:CDS 40.0%
GAGGGAAACAAATGAGGTTT+AGG - chr4.2:22459465-22459484 MS.gene39482:intron 40.0%
GCCTTCATTTGAGCATTTGA+TGG - chr4.2:22460721-22460740 MS.gene39482:intron 40.0%
GGAAAAGATAAGAGATGGGT+TGG - chr4.2:22459397-22459416 MS.gene39482:intron 40.0%
GGATCCCTATGGAAGAATTA+TGG - chr4.2:22461373-22461392 MS.gene39482:CDS 40.0%
GGATGAAAAAATTGGGTGGT+TGG - chr4.2:22459919-22459938 MS.gene39482:intron 40.0%
GGCATGATAGTCAAAGGTAA+AGG - chr4.2:22459998-22460017 MS.gene39482:intron 40.0%
GTTACGAAGGTTGTTAAAGG+AGG - chr4.2:22459446-22459465 MS.gene39482:intron 40.0%
TGACATTGTGGCCTATATAG+TGG - chr4.2:22460575-22460594 MS.gene39482:intron 40.0%
TGTCATGCATAATGCAATGC+TGG + chr4.2:22460423-22460442 None:intergenic 40.0%
TTCTAGCATCTTAGAACACG+GGG + chr4.2:22461063-22461082 None:intergenic 40.0%
TTGACCAATGGGAAACTTGT+TGG - chr4.2:22459848-22459867 MS.gene39482:intron 40.0%
! AATGTGAGTTTGTGGGTTGT+GGG + chr4.2:22459068-22459087 None:intergenic 40.0%
! GGGTACCTCATTTGAAATTC+AGG - chr4.2:22460231-22460250 MS.gene39482:intron 40.0%
! TATTCAGCTTTGGTATCCCT+CGG + chr4.2:22460753-22460772 None:intergenic 40.0%
!! AATGCTTTGGGGAAACAACT+AGG - chr4.2:22461264-22461283 MS.gene39482:CDS 40.0%
!! AGAATGGAGTTTTGGTGAGA+GGG + chr4.2:22459091-22459110 None:intergenic 40.0%
!! AGAGAATTGAGTGCTGAAGA+AGG + chr4.2:22458993-22459012 None:intergenic 40.0%
!! GATGGTGTTGTCGAATTCAT+CGG + chr4.2:22459156-22459175 None:intergenic 40.0%
!! GTTGTTGTTGGTGATAAGCA+AGG - chr4.2:22459494-22459513 MS.gene39482:intron 40.0%
!! TGTTTCTGGCTTTCTGCTAT+AGG - chr4.2:22460501-22460520 MS.gene39482:intron 40.0%
AACACGTATGCAGCGATCTA+CGG + chr4.2:22460833-22460852 None:intergenic 45.0%
AAGAGGAGGAAGAAGAGGAA+CGG + chr4.2:22459031-22459050 None:intergenic 45.0%
ACCGTATAGCTCTTCGTATG+CGG + chr4.2:22459282-22459301 None:intergenic 45.0%
AGTCTCTTCTTGCTGAGAGA+TGG - chr4.2:22460991-22461010 MS.gene39482:CDS 45.0%
ATACGAAGAGCTATACGGTG+TGG - chr4.2:22459283-22459302 MS.gene39482:intron 45.0%
GAAACAAGAGGAGGAAGAAG+AGG + chr4.2:22459036-22459055 None:intergenic 45.0%
GAAGAAGGAATTCTCCACCA+AGG + chr4.2:22460030-22460049 None:intergenic 45.0%
GAATGCTTATCGCAGCTGAA+AGG - chr4.2:22460867-22460886 MS.gene39482:CDS 45.0%
GAGAGGGAATGTGAGTTTGT+GGG + chr4.2:22459075-22459094 None:intergenic 45.0%
GCAATGCCCTTCTTATATGG+TGG - chr4.2:22460526-22460545 MS.gene39482:intron 45.0%
GTCGCAATGCCCTTCTTATA+TGG - chr4.2:22460523-22460542 MS.gene39482:intron 45.0%
TAGATCGCTGCATACGTGTT+TGG - chr4.2:22460833-22460852 MS.gene39482:CDS 45.0%
TCCAGAGCTGATGGAGATTA+TGG - chr4.2:22461147-22461166 MS.gene39482:CDS 45.0%
TCCATAATCTCCATCAGCTC+TGG + chr4.2:22461151-22461170 None:intergenic 45.0%
TGAAGGGAAGGTCTTCTTCT+GGG + chr4.2:22459178-22459197 None:intergenic 45.0%
TGAGAGGGAATGTGAGTTTG+TGG + chr4.2:22459076-22459095 None:intergenic 45.0%
TGCATGACAGATTCTGTGCT+AGG - chr4.2:22460434-22460453 MS.gene39482:intron 45.0%
TGGAACTGGACTCTGATGTA+AGG - chr4.2:22459877-22459896 MS.gene39482:intron 45.0%
TTGAAGGGAAGGTCTTCTTC+TGG + chr4.2:22459179-22459198 None:intergenic 45.0%
TTGTCGAATTCATCGGAGGT+GGG + chr4.2:22459149-22459168 None:intergenic 45.0%
! AGGTCACACACGAAGTATCA+AGG + chr4.2:22460141-22460160 None:intergenic 45.0%
! ATGTGAGTTTGTGGGTTGTG+GGG + chr4.2:22459067-22459086 None:intergenic 45.0%
! ATTCAGCTTTGGTATCCCTC+GGG + chr4.2:22460752-22460771 None:intergenic 45.0%
! GAATGTGAGTTTGTGGGTTG+TGG + chr4.2:22459069-22459088 None:intergenic 45.0%
! GGAGAGAGAGAATGGAGTTT+TGG + chr4.2:22459099-22459118 None:intergenic 45.0%
! TACAGTGGTGTTAGTGTGCT+TGG - chr4.2:22459308-22459327 MS.gene39482:intron 45.0%
! TGGAGATTATGGAGCGTCAA+AGG - chr4.2:22461158-22461177 MS.gene39482:CDS 45.0%
!! GAGAATGGAGTTTTGGTGAG+AGG + chr4.2:22459092-22459111 None:intergenic 45.0%
!! TGTTGGTGTTGGGAAAGCTA+AGG - chr4.2:22459526-22459545 MS.gene39482:intron 45.0%
AAGATAGTCGCTGCTGCCTA+TGG + chr4.2:22460462-22460481 None:intergenic 50.0%
AGACCCACCTGCAATAACTC+CGG + chr4.2:22461211-22461230 None:intergenic 50.0%
ATGAAGCCTTCCGGGATTTC+CGG + chr4.2:22459206-22459225 None:intergenic 50.0%
CATCAGAGTCCAGTTCCACT+CGG + chr4.2:22459875-22459894 None:intergenic 50.0%
CGGACCAACAAGTTTCCCAT+TGG + chr4.2:22459855-22459874 None:intergenic 50.0%
GAAGCCAGACCTGTCTGATT+AGG - chr4.2:22460372-22460391 MS.gene39482:intron 50.0%
GACACGGAACCTAATCAGAC+AGG + chr4.2:22460384-22460403 None:intergenic 50.0%
GAGAGAGTGGAGAGAGAGAA+TGG + chr4.2:22459107-22459126 None:intergenic 50.0%
GAGGAGAGAGAAACAAGAGG+AGG + chr4.2:22459045-22459064 None:intergenic 50.0%
GCCGCATACGAAGAGCTATA+CGG - chr4.2:22459278-22459297 MS.gene39482:intron 50.0%
GGAACCTAATCAGACAGGTC+TGG + chr4.2:22460379-22460398 None:intergenic 50.0%
GGTAAAGGGGAAATCCTTGG+TGG - chr4.2:22460013-22460032 MS.gene39482:intron 50.0%
GGTGATAAGCAAGGACGAGT+TGG - chr4.2:22459503-22459522 MS.gene39482:intron 50.0%
GTACCGGAGTTATTGCAGGT+GGG - chr4.2:22461205-22461224 MS.gene39482:CDS 50.0%
GTATAGCTCTTCGTATGCGG+CGG + chr4.2:22459279-22459298 None:intergenic 50.0%
GTTGTCGAATTCATCGGAGG+TGG + chr4.2:22459150-22459169 None:intergenic 50.0%
TCAAGTGAGGAGGGTTACGA+AGG - chr4.2:22459433-22459452 MS.gene39482:intron 50.0%
! CATTTGATGGTTCTCCCCGA+GGG - chr4.2:22460734-22460753 MS.gene39482:intron 50.0%
! CCCCAAAGCATTCTCAACAC+CGG + chr4.2:22461256-22461275 None:intergenic 50.0%
! CCGGTGTTGAGAATGCTTTG+GGG - chr4.2:22461253-22461272 MS.gene39482:CDS 50.0%
! GATTCTGTGCTAGGTGCCAT+AGG - chr4.2:22460443-22460462 MS.gene39482:intron 50.0%
! GCCGGTGTTGAGAATGCTTT+GGG - chr4.2:22461252-22461271 MS.gene39482:CDS 50.0%
! GGTGTTGTCGAATTCATCGG+AGG + chr4.2:22459153-22459172 None:intergenic 50.0%
! TTCAGCTTTGGTATCCCTCG+GGG + chr4.2:22460751-22460770 None:intergenic 50.0%
!! AAGCAAGGACGAGTTGGTGT+TGG - chr4.2:22459509-22459528 MS.gene39482:intron 50.0%
!! ATGTGATGGATAAGACGGCG+AGG - chr4.2:22459342-22459361 MS.gene39482:intron 50.0%
!! GACGAGTTGGTGTTGGTGTT+GGG - chr4.2:22459516-22459535 MS.gene39482:intron 50.0%
AACTCCGGTACCAGGAGAAG+CGG + chr4.2:22461196-22461215 None:intergenic 55.0%
AAGACGGCGAGGAAGGATAC+CGG - chr4.2:22459353-22459372 MS.gene39482:intron 55.0%
ACTGGACCGTCGTCTATGGA+AGG + chr4.2:22459236-22459255 None:intergenic 55.0%
AGACCTTCCCTTCAACCCAC+CGG - chr4.2:22459184-22459203 MS.gene39482:CDS 55.0%
ATGCTTAGACCCGCTTCTCC+TGG - chr4.2:22461183-22461202 MS.gene39482:CDS 55.0%
CACTGCAGCTACAGTTGCTC+TGG + chr4.2:22461313-22461332 None:intergenic 55.0%
CCACCACCTTCCATAGACGA+CGG - chr4.2:22459227-22459246 MS.gene39482:intron 55.0%
CCTGCAATAACTCCGGTACC+AGG + chr4.2:22461204-22461223 None:intergenic 55.0%
CTATACGGTGTGGCGTACAG+TGG - chr4.2:22459293-22459312 MS.gene39482:intron 55.0%
CTTCAACCCACCGGAAATCC+CGG - chr4.2:22459193-22459212 MS.gene39482:CDS 55.0%
CTTGTTGGTCCGAGTGGAAC+TGG - chr4.2:22459863-22459882 MS.gene39482:intron 55.0%
GATGGATAAGACGGCGAGGA+AGG - chr4.2:22459346-22459365 MS.gene39482:intron 55.0%
GGGAATTGCTTTGACCGCGA+AGG + chr4.2:22459129-22459148 None:intergenic 55.0%
GGGGAGGAGAGAGAAACAAG+AGG + chr4.2:22459048-22459067 None:intergenic 55.0%
TCTCTCCACTCTCTCCTTCG+CGG - chr4.2:22459112-22459131 MS.gene39482:CDS 55.0%
TTCGACTGGACCGTCGTCTA+TGG + chr4.2:22459240-22459259 None:intergenic 55.0%
TTTGACCGCGAAGGAGAGAG+TGG + chr4.2:22459120-22459139 None:intergenic 55.0%
! AAGGATACCGGGTTCGGTTC+GGG - chr4.2:22459365-22459384 MS.gene39482:intron 55.0%
! CCTGGTACCGGAGTTATTGC+AGG - chr4.2:22461201-22461220 MS.gene39482:CDS 55.0%
! GAAGGATACCGGGTTCGGTT+CGG - chr4.2:22459364-22459383 MS.gene39482:intron 55.0%
! GCATTTGATGGTTCTCCCCG+AGG - chr4.2:22460733-22460752 MS.gene39482:intron 55.0%
! GGCCGGTGTTGAGAATGCTT+TGG - chr4.2:22461251-22461270 MS.gene39482:CDS 55.0%
! GGGAAGGTCTTCTTCTGGGA+TGG + chr4.2:22459174-22459193 None:intergenic 55.0%
! GGTACCGGAGTTATTGCAGG+TGG - chr4.2:22461204-22461223 MS.gene39482:CDS 55.0%
! GTGGTGGAATGAAGCCTTCC+GGG + chr4.2:22459214-22459233 None:intergenic 55.0%
! TGAGTTTGTGGGTTGTGGGG+AGG + chr4.2:22459064-22459083 None:intergenic 55.0%
!! GGACGAGTTGGTGTTGGTGT+TGG - chr4.2:22459515-22459534 MS.gene39482:intron 55.0%
!! GGGAAACTTGTTGGTCCGAG+TGG - chr4.2:22459857-22459876 MS.gene39482:intron 55.0%
!! GGGATTTCCGGTGGGTTGAA+GGG + chr4.2:22459194-22459213 None:intergenic 55.0%
!! GGTGGTGGAATGAAGCCTTC+CGG + chr4.2:22459215-22459234 None:intergenic 55.0%
!! TTTCCGGTGGGTTGAAGGGA+AGG + chr4.2:22459190-22459209 None:intergenic 55.0%
AACCCACCGGAAATCCCGGA+AGG - chr4.2:22459197-22459216 MS.gene39482:CDS 60.0%
AAGCCTTCCGGGATTTCCGG+TGG + chr4.2:22459203-22459222 None:intergenic 60.0%
ACTCCGGTACCAGGAGAAGC+GGG + chr4.2:22461195-22461214 None:intergenic 60.0%
AGACCCGCTTCTCCTGGTAC+CGG - chr4.2:22461189-22461208 MS.gene39482:CDS 60.0%
AGACGGCGAGGAAGGATACC+GGG - chr4.2:22459354-22459373 MS.gene39482:intron 60.0%
AGCCTTCCGGGATTTCCGGT+GGG + chr4.2:22459202-22459221 None:intergenic 60.0%
CCGTCGTCTATGGAAGGTGG+TGG + chr4.2:22459230-22459249 None:intergenic 60.0%
GCAGTACAGTGAGGTCGCTG+AGG - chr4.2:22461344-22461363 MS.gene39482:CDS 60.0%
GCGAGGAAGGATACCGGGTT+CGG - chr4.2:22459359-22459378 MS.gene39482:intron 60.0%
GGACCGTCGTCTATGGAAGG+TGG + chr4.2:22459233-22459252 None:intergenic 60.0%
! TGAGGAGCGTGGGATCCCTA+TGG - chr4.2:22461362-22461381 MS.gene39482:CDS 60.0%
!! CGGGATTTCCGGTGGGTTGA+AGG + chr4.2:22459195-22459214 None:intergenic 60.0%
!! GTTCGGTTCGGGTGCGAAGA+AGG - chr4.2:22459376-22459395 MS.gene39482:intron 60.0%
AGTGAGGTCGCTGAGGAGCG+TGG - chr4.2:22461351-22461370 MS.gene39482:CDS 65.0%
CTTCGCACCCGAACCGAACC+CGG + chr4.2:22459375-22459394 None:intergenic 65.0%
! GTGAGGTCGCTGAGGAGCGT+GGG - chr4.2:22461352-22461371 MS.gene39482:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 22458978 22461401 22458978 ID=MS.gene39482
chr4.2 mRNA 22458978 22461401 22458978 ID=MS.gene39482.t1;Parent=MS.gene39482
chr4.2 exon 22460740 22461401 22460740 ID=MS.gene39482.t1.exon1;Parent=MS.gene39482.t1
chr4.2 CDS 22460740 22461401 22460740 ID=cds.MS.gene39482.t1;Parent=MS.gene39482.t1
chr4.2 exon 22458978 22459227 22458978 ID=MS.gene39482.t1.exon2;Parent=MS.gene39482.t1
chr4.2 CDS 22458978 22459227 22458978 ID=cds.MS.gene39482.t1;Parent=MS.gene39482.t1
Gene Sequence

>MS.gene39482

ATGGCTGCTTCACCTTCTTCAGCACTCAATTCTCTCTCTTCTCTCTCTTTCCGTTCCTCTTCTTCCTCCTCTTGTTTCTCTCTCCTCCCCACAACCCACAAACTCACATTCCCTCTCACCAAAACTCCATTCTCTCTCTCCACTCTCTCCTTCGCGGTCAAAGCAATTCCCACCTCCGATGAATTCGACAACACCATCCCAGAAGAAGACCTTCCCTTCAACCCACCGGAAATCCCGGAAGGCTTCATTCCACCACCTTCCATAGACGACGGTCCAGTCGAAGACGAAGATGAAATCGCCGCCGCATACGAAGAGCTATACGGTGTGGCGTACAGTGGTGTTAGTGTGCTTGGAAATGATATAAATGTGATGGATAAGACGGCGAGGAAGGATACCGGGTTCGGTTCGGGTGCGAAGAAGGAAAAGATAAGAGATGGGTTGGAAGAAAGAGTTGTTCAAGTGAGGAGGGTTACGAAGGTTGTTAAAGGAGGGAAACAAATGAGGTTTAGGGCTATTGTTGTTGTTGGTGATAAGCAAGGACGAGTTGGTGTTGGTGTTGGGAAAGCTAAGGAAGTTGTTTCTGCTGTTCAGAAATCTGCTATTAATGCTAGGAGGAATCTTATTAGAGTTCCCATGACCAAGTACTTGACTTTTCCTCATAGGTAACTTTCTCTAACTTTCATATATTCATCAATCTTAATTTTGGTTTTATCATTTTTCATTACATGTTTTGCTTAAATGACATTAGACGTAATTTGATCCATGTAACCGAACTCACTTAGTGGAATAAGGTTTTGTTGTTGTTGTTGTTGATCCACGTAAATGAGATTAGTTTAGCGAATTATAATGTCATTAGAAATGAGAAACTTGTTGACCAATGGGAAACTTGTTGGTCCGAGTGGAACTGGACTCTGATGTAAGGTTTTGAATTTGAAACTTATGGATGAAAAAATTGGGTGGTTGGTTTGATTCTTTTGTAGAGATTAGTCATTAGCATAGTTGGCAAATGCTTTGACATATGGCATGATAGTCAAAGGTAAAGGGGAAATCCTTGGTGGAGAATTCCTTCTTCTATTCTCAATGGAACAATGAAATTGTTCCTTACCTTTGCTTGTTTCTATCGAGTTTTCGTTTTTCCTTCTTGTGTTCTTAACATTGTTCCTTGATACTTCGTGTGTGACCTTGAATTGCATTATTCATAATTCACATTGTATTAGAGCTTCATTTGGATATTTGTGTATATAGGAGTTATTGGGTACCTCATTTGAAATTCAGGAAAACATTTCACAATATAATGAAGCATTCTTGTGGCTTGATTCTAATATTATCATGATTACTTTGTGTACTGAACATTGAGGTAGAACAGACTCGGCTAATTTTTCTTTGACATGATGGAAGCCAGACCTGTCTGATTAGGTTCCGTGTCTCACATTGTCAAATTGCCAGCATTGCATTATGCATGACAGATTCTGTGCTAGGTGCCATAGGCAGCAGCGACTATCTTAATCTTATCATTATCACTATGTTTCTGGCTTTCTGCTATAGGTCGCAATGCCCTTCTTATATGGTGGATATTTGTCTGGCCTCCATATTAATATGACATTGTGGCCTATATAGTGGAATCTGCTTTGTGGATGATTCACTATGTTGACATGTGACTACCTGTAATGCTCTATGTAAAAATGAGAAACGAATATGAAATATGGACATTCAATTTGCTATTAGCATCGTAAGATTTTACTGGCCTTCATTTGAGCATTTGATGGTTCTCCCCGAGGGATACCAAAGCTGAATATAACGCATCTGATTGAGGTTGTAGTTGGAGCATGCATTTTCAACTAGCAGTTCTCTGCCGTAGATCGCTGCATACGTGTTTGGTGCCCATTTGTGAATGCTTATCGCAGCTGAAAGGGCTCTTGCTGATGAATGCCTAAGTAACTTATGGTCACTTAAGCAACCTATTAAGATGTTCCCTGTTAAATTTCCTTAACTCAAATAGGAACTGAAGAGCAGAGTCTCTTCTTGCTGAGAGATGGAACTTTTATTTCAAAGGAGAAGCATATCAATAACTTTGACTGAGTTCCCCGTGTTCTAAGATGCTAGAATTTTAGTTAGATATGAATTTTGAGTTGCTAATTGCCACTAATTTTATGTCGTATATAATGTTTTTCCAGAGCTGATGGAGATTATGGAGCGTCAAAGGTGATGCTTAGACCCGCTTCTCCTGGTACCGGAGTTATTGCAGGTGGGTCTGTGAGAATTGTTCTTGAAATGGCCGGTGTTGAGAATGCTTTGGGGAAACAACTAGGAAGTGACAATGCACTCAACAATGCCAGAGCAACTGTAGCTGCAGTGCAGAAAATGAAGCAGTACAGTGAGGTCGCTGAGGAGCGTGGGATCCCTATGGAAGAATTATGGAAGTAG
Protein sequence

>MS.gene39482.t1

MAASPSSALNSLSSLSFRSSSSSSCFSLLPTTHKLTFPLTKTPFSLSTLSFAVKAIPTSDEFDNTIPEEDLPFNPPEIPEGFIPPPSIDDGPVEDEDEIAAAYEELYGVAYSGVSVLGNDINVMDKTARKDTGFGSGAKKEKIRDGLEERVVQVRRVTKVVKGGKQMRFRAIVVVGDKQGRVGVGVGKAKEVVSAVQKSAINARRNLIRVPMTKYLTFPHRADGDYGASKVMLRPASPGTGVIAGGSVRIVLEMAGVENALGKQLGSDNALNNARATVAAVQKMKQYSEVAEERGIPMEELWK