Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57034.t1 | XP_013467015.1 | 94.6 | 112 | 6 | 0 | 1 | 112 | 35 | 146 | 1.00E-50 | 209.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57034.t1 | O22795 | 81.2 | 117 | 14 | 2 | 1 | 112 | 30 | 143 | 2.0e-42 | 172.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57034.t1 | I3SVT1 | 94.6 | 112 | 6 | 0 | 1 | 112 | 35 | 146 | 7.4e-51 | 209.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene57034 | 0.900459 | 7.17E-78 | -1.69E-46 |
| MS.gene049173 | MS.gene57034 | 0.870646 | 1.25E-66 | -1.69E-46 |
| MS.gene049217 | MS.gene57034 | 0.864842 | 9.09E-65 | -1.69E-46 |
| MS.gene049222 | MS.gene57034 | 0.886437 | 3.42E-72 | -1.69E-46 |
| MS.gene049232 | MS.gene57034 | 0.863428 | 2.51E-64 | -1.69E-46 |
| MS.gene049281 | MS.gene57034 | -0.803756 | 3.00E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene57034 | 0.930857 | 8.88E-94 | -1.69E-46 |
| MS.gene049320 | MS.gene57034 | 0.917918 | 2.95E-86 | -1.69E-46 |
| MS.gene04939 | MS.gene57034 | 0.824336 | 8.53E-54 | -1.69E-46 |
| MS.gene049609 | MS.gene57034 | 0.822506 | 2.28E-53 | -1.69E-46 |
| MS.gene049627 | MS.gene57034 | 0.858838 | 6.26E-63 | -1.69E-46 |
| MS.gene049633 | MS.gene57034 | 0.832592 | 8.66E-56 | -1.69E-46 |
| MS.gene049805 | MS.gene57034 | 0.807832 | 4.17E-50 | -1.69E-46 |
| MS.gene04986 | MS.gene57034 | -0.807374 | 5.22E-50 | -1.69E-46 |
| MS.gene05010 | MS.gene57034 | 0.879581 | 1.11E-69 | -1.69E-46 |
| MS.gene050163 | MS.gene57034 | 0.935789 | 4.87E-97 | -1.69E-46 |
| MS.gene050165 | MS.gene57034 | 0.939756 | 7.43E-100 | -1.69E-46 |
| MS.gene050170 | MS.gene57034 | 0.81524 | 1.02E-51 | -1.69E-46 |
| MS.gene050216 | MS.gene57034 | 0.819055 | 1.42E-52 | -1.69E-46 |
| MS.gene050323 | MS.gene57034 | 0.851068 | 1.13E-60 | -1.69E-46 |
| MS.gene050333 | MS.gene57034 | 0.919099 | 6.87E-87 | -1.69E-46 |
| MS.gene050444 | MS.gene57034 | 0.853159 | 2.87E-61 | -1.69E-46 |
| MS.gene050496 | MS.gene57034 | 0.846168 | 2.57E-59 | -1.69E-46 |
| MS.gene050504 | MS.gene57034 | 0.928439 | 2.88E-92 | -1.69E-46 |
| MS.gene050518 | MS.gene57034 | 0.901128 | 3.67E-78 | -1.69E-46 |
| MS.gene050577 | MS.gene57034 | 0.855364 | 6.63E-62 | -1.69E-46 |
| MS.gene050612 | MS.gene57034 | 0.917438 | 5.31E-86 | -1.69E-46 |
| MS.gene050675 | MS.gene57034 | 0.880822 | 4.00E-70 | -1.69E-46 |
| MS.gene050699 | MS.gene57034 | 0.891138 | 5.22E-74 | -1.69E-46 |
| MS.gene050747 | MS.gene57034 | 0.813423 | 2.58E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene57034 | 0.864102 | 1.55E-64 | -1.69E-46 |
| MS.gene05079 | MS.gene57034 | -0.809355 | 1.97E-50 | -1.69E-46 |
| MS.gene05080 | MS.gene57034 | 0.814603 | 1.42E-51 | -1.69E-46 |
| MS.gene050832 | MS.gene57034 | -0.823435 | 1.39E-53 | -1.69E-46 |
| MS.gene050833 | MS.gene57034 | -0.817391 | 3.38E-52 | -1.69E-46 |
| MS.gene050868 | MS.gene57034 | 0.849599 | 2.92E-60 | -1.69E-46 |
| MS.gene050871 | MS.gene57034 | 0.846099 | 2.69E-59 | -1.69E-46 |
| MS.gene050876 | MS.gene57034 | 0.883202 | 5.48E-71 | -1.69E-46 |
| MS.gene05087 | MS.gene57034 | 0.836671 | 8.16E-57 | -1.69E-46 |
| MS.gene050904 | MS.gene57034 | 0.834326 | 3.20E-56 | -1.69E-46 |
| MS.gene050950 | MS.gene57034 | 0.810094 | 1.37E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene57034 | 0.902225 | 1.21E-78 | -1.69E-46 |
| MS.gene05100 | MS.gene57034 | 0.899202 | 2.50E-77 | -1.69E-46 |
| MS.gene051127 | MS.gene57034 | -0.812315 | 4.51E-51 | -1.69E-46 |
| MS.gene051138 | MS.gene57034 | -0.8092 | 2.13E-50 | -1.69E-46 |
| MS.gene051228 | MS.gene57034 | 0.833584 | 4.90E-56 | -1.69E-46 |
| MS.gene051268 | MS.gene57034 | 0.813438 | 2.56E-51 | -1.69E-46 |
| MS.gene051317 | MS.gene57034 | 0.856757 | 2.59E-62 | -1.69E-46 |
| MS.gene051364 | MS.gene57034 | -0.81404 | 1.89E-51 | -1.69E-46 |
| MS.gene051366 | MS.gene57034 | -0.829581 | 4.75E-55 | -1.69E-46 |
| MS.gene051367 | MS.gene57034 | -0.800479 | 1.41E-48 | -1.69E-46 |
| MS.gene051551 | MS.gene57034 | 0.883222 | 5.39E-71 | -1.69E-46 |
| MS.gene051564 | MS.gene57034 | 0.821046 | 4.97E-53 | -1.69E-46 |
| MS.gene051566 | MS.gene57034 | 0.804233 | 2.38E-49 | -1.69E-46 |
| MS.gene051694 | MS.gene57034 | 0.825129 | 5.55E-54 | -1.69E-46 |
| MS.gene051810 | MS.gene57034 | 0.884593 | 1.68E-71 | -1.69E-46 |
| MS.gene051858 | MS.gene57034 | 0.822702 | 2.06E-53 | -1.69E-46 |
| MS.gene051949 | MS.gene57034 | 0.88713 | 1.87E-72 | -1.69E-46 |
| MS.gene052129 | MS.gene57034 | -0.808342 | 3.25E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene57034 | 0.833698 | 4.59E-56 | -1.69E-46 |
| MS.gene052271 | MS.gene57034 | 0.851538 | 8.32E-61 | -1.69E-46 |
| MS.gene052379 | MS.gene57034 | 0.836863 | 7.29E-57 | -1.69E-46 |
| MS.gene052482 | MS.gene57034 | 0.822035 | 2.94E-53 | -1.69E-46 |
| MS.gene052486 | MS.gene57034 | 0.828805 | 7.33E-55 | -1.69E-46 |
| MS.gene052503 | MS.gene57034 | 0.805245 | 1.46E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene57034 | 0.819075 | 1.41E-52 | -1.69E-46 |
| MS.gene052822 | MS.gene57034 | 0.816022 | 6.85E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene57034 | 0.800482 | 1.41E-48 | -1.69E-46 |
| MS.gene052870 | MS.gene57034 | 0.807572 | 4.73E-50 | -1.69E-46 |
| MS.gene052872 | MS.gene57034 | 0.822029 | 2.95E-53 | -1.69E-46 |
| MS.gene052916 | MS.gene57034 | 0.86065 | 1.78E-63 | -1.69E-46 |
| MS.gene052966 | MS.gene57034 | 0.841409 | 4.84E-58 | -1.69E-46 |
| MS.gene053203 | MS.gene57034 | 0.852156 | 5.55E-61 | -1.69E-46 |
| MS.gene053213 | MS.gene57034 | 0.877008 | 8.87E-69 | -1.69E-46 |
| MS.gene053284 | MS.gene57034 | 0.845162 | 4.82E-59 | -1.69E-46 |
| MS.gene053285 | MS.gene57034 | 0.830093 | 3.57E-55 | -1.69E-46 |
| MS.gene053286 | MS.gene57034 | 0.879383 | 1.30E-69 | -1.69E-46 |
| MS.gene053322 | MS.gene57034 | 0.811221 | 7.80E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene57034 | -0.809996 | 1.44E-50 | -1.69E-46 |
| MS.gene053561 | MS.gene57034 | -0.808731 | 2.68E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene57034 | 0.821571 | 3.76E-53 | -1.69E-46 |
| MS.gene053632 | MS.gene57034 | 0.845387 | 4.19E-59 | -1.69E-46 |
| MS.gene053824 | MS.gene57034 | 0.827633 | 1.40E-54 | -1.69E-46 |
| MS.gene053916 | MS.gene57034 | 0.818657 | 1.75E-52 | -1.69E-46 |
| MS.gene054408 | MS.gene57034 | 0.805495 | 1.30E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene57034 | 0.841473 | 4.65E-58 | -1.69E-46 |
| MS.gene054600 | MS.gene57034 | 0.859892 | 3.02E-63 | -1.69E-46 |
| MS.gene054616 | MS.gene57034 | -0.821482 | 3.95E-53 | -1.69E-46 |
| MS.gene054642 | MS.gene57034 | 0.908894 | 1.04E-81 | -1.69E-46 |
| MS.gene05470 | MS.gene57034 | 0.870974 | 9.73E-67 | -1.69E-46 |
| MS.gene054954 | MS.gene57034 | 0.932365 | 9.51E-95 | -1.69E-46 |
| MS.gene054959 | MS.gene57034 | -0.804351 | 2.25E-49 | -1.69E-46 |
| MS.gene055134 | MS.gene57034 | -0.800597 | 1.34E-48 | -1.69E-46 |
| MS.gene055154 | MS.gene57034 | -0.828261 | 9.92E-55 | -1.69E-46 |
| MS.gene055204 | MS.gene57034 | -0.80837 | 3.20E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene57034 | 0.806781 | 6.96E-50 | -1.69E-46 |
| MS.gene055212 | MS.gene57034 | 0.821547 | 3.81E-53 | -1.69E-46 |
| MS.gene055362 | MS.gene57034 | 0.919237 | 5.78E-87 | -1.69E-46 |
| MS.gene055397 | MS.gene57034 | 0.807794 | 4.25E-50 | -1.69E-46 |
| MS.gene055499 | MS.gene57034 | 0.822388 | 2.43E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene57034 | 0.89675 | 2.73E-76 | -1.69E-46 |
| MS.gene055577 | MS.gene57034 | 0.872046 | 4.29E-67 | -1.69E-46 |
| MS.gene055634 | MS.gene57034 | 0.850437 | 1.70E-60 | -1.69E-46 |
| MS.gene055636 | MS.gene57034 | 0.839525 | 1.50E-57 | -1.69E-46 |
| MS.gene055638 | MS.gene57034 | 0.853375 | 2.49E-61 | -1.69E-46 |
| MS.gene055647 | MS.gene57034 | 0.810604 | 1.06E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene57034 | 0.835937 | 1.25E-56 | -1.69E-46 |
| MS.gene055748 | MS.gene57034 | 0.880136 | 7.04E-70 | -1.69E-46 |
| MS.gene055750 | MS.gene57034 | 0.858009 | 1.11E-62 | -1.69E-46 |
| MS.gene055751 | MS.gene57034 | 0.892357 | 1.71E-74 | -1.69E-46 |
| MS.gene055776 | MS.gene57034 | 0.888818 | 4.21E-73 | -1.69E-46 |
| MS.gene055849 | MS.gene57034 | 0.849025 | 4.21E-60 | -1.69E-46 |
| MS.gene05589 | MS.gene57034 | 0.847555 | 1.07E-59 | -1.69E-46 |
| MS.gene05592 | MS.gene57034 | 0.801432 | 9.03E-49 | -1.69E-46 |
| MS.gene055941 | MS.gene57034 | 0.842751 | 2.13E-58 | -1.69E-46 |
| MS.gene055942 | MS.gene57034 | -0.820584 | 6.35E-53 | -1.69E-46 |
| MS.gene055956 | MS.gene57034 | -0.834916 | 2.27E-56 | -1.69E-46 |
| MS.gene056083 | MS.gene57034 | 0.851574 | 8.12E-61 | -1.69E-46 |
| MS.gene056374 | MS.gene57034 | 0.873504 | 1.40E-67 | -1.69E-46 |
| MS.gene056504 | MS.gene57034 | 0.890284 | 1.13E-73 | -1.69E-46 |
| MS.gene056510 | MS.gene57034 | 0.888481 | 5.68E-73 | -1.69E-46 |
| MS.gene056549 | MS.gene57034 | 0.839449 | 1.57E-57 | -1.69E-46 |
| MS.gene056550 | MS.gene57034 | -0.814458 | 1.52E-51 | -1.69E-46 |
| MS.gene056610 | MS.gene57034 | 0.802583 | 5.24E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene57034 | 0.915866 | 3.53E-85 | -1.69E-46 |
| MS.gene056692 | MS.gene57034 | 0.879496 | 1.19E-69 | -1.69E-46 |
| MS.gene056761 | MS.gene57034 | -0.825646 | 4.19E-54 | -1.69E-46 |
| MS.gene056898 | MS.gene57034 | 0.841089 | 5.87E-58 | -1.69E-46 |
| MS.gene057129 | MS.gene57034 | 0.84655 | 2.02E-59 | -1.69E-46 |
| MS.gene057148 | MS.gene57034 | 0.800076 | 1.71E-48 | -1.69E-46 |
| MS.gene057246 | MS.gene57034 | 0.809058 | 2.28E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene57034 | 0.811199 | 7.89E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene57034 | 0.85025 | 1.92E-60 | -1.69E-46 |
| MS.gene057658 | MS.gene57034 | 0.814768 | 1.30E-51 | -1.69E-46 |
| MS.gene057831 | MS.gene57034 | 0.850662 | 1.47E-60 | -1.69E-46 |
| MS.gene057986 | MS.gene57034 | 0.83738 | 5.38E-57 | -1.69E-46 |
| MS.gene058268 | MS.gene57034 | -0.820493 | 6.66E-53 | -1.69E-46 |
| MS.gene058269 | MS.gene57034 | -0.819731 | 9.96E-53 | -1.69E-46 |
| MS.gene058334 | MS.gene57034 | -0.829422 | 5.19E-55 | -1.69E-46 |
| MS.gene058335 | MS.gene57034 | 0.830793 | 2.40E-55 | -1.69E-46 |
| MS.gene05837 | MS.gene57034 | 0.823096 | 1.67E-53 | -1.69E-46 |
| MS.gene058419 | MS.gene57034 | 0.910143 | 2.60E-82 | -1.69E-46 |
| MS.gene058544 | MS.gene57034 | 0.870765 | 1.14E-66 | -1.69E-46 |
| MS.gene058719 | MS.gene57034 | 0.823009 | 1.74E-53 | -1.69E-46 |
| MS.gene058723 | MS.gene57034 | 0.840928 | 6.47E-58 | -1.69E-46 |
| MS.gene058773 | MS.gene57034 | 0.907208 | 6.49E-81 | -1.69E-46 |
| MS.gene058838 | MS.gene57034 | 0.833793 | 4.35E-56 | -1.69E-46 |
| MS.gene059022 | MS.gene57034 | 0.936824 | 9.34E-98 | -1.69E-46 |
| MS.gene059023 | MS.gene57034 | 0.868351 | 6.96E-66 | -1.69E-46 |
| MS.gene059029 | MS.gene57034 | 0.83857 | 2.66E-57 | -1.69E-46 |
| MS.gene059079 | MS.gene57034 | 0.830815 | 2.37E-55 | -1.69E-46 |
| MS.gene05912 | MS.gene57034 | 0.854557 | 1.14E-61 | -1.69E-46 |
| MS.gene05913 | MS.gene57034 | 0.83328 | 5.84E-56 | -1.69E-46 |
| MS.gene05934 | MS.gene57034 | 0.802144 | 6.45E-49 | -1.69E-46 |
| MS.gene059479 | MS.gene57034 | -0.825115 | 5.59E-54 | -1.69E-46 |
| MS.gene059677 | MS.gene57034 | 0.935866 | 4.32E-97 | -1.69E-46 |
| MS.gene059718 | MS.gene57034 | 0.883874 | 3.10E-71 | -1.69E-46 |
| MS.gene059756 | MS.gene57034 | 0.858536 | 7.70E-63 | -1.69E-46 |
| MS.gene059801 | MS.gene57034 | 0.837014 | 6.67E-57 | -1.69E-46 |
| MS.gene059837 | MS.gene57034 | 0.80592 | 1.06E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene57034 | 0.897648 | 1.15E-76 | -1.69E-46 |
| MS.gene059902 | MS.gene57034 | 0.820613 | 6.25E-53 | -1.69E-46 |
| MS.gene059960 | MS.gene57034 | 0.812857 | 3.43E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene57034 | 0.80601 | 1.01E-49 | -1.69E-46 |
| MS.gene060337 | MS.gene57034 | -0.862709 | 4.18E-64 | -1.69E-46 |
| MS.gene060341 | MS.gene57034 | 0.819681 | 1.02E-52 | -1.69E-46 |
| MS.gene060482 | MS.gene57034 | 0.806822 | 6.83E-50 | -1.69E-46 |
| MS.gene060602 | MS.gene57034 | -0.800785 | 1.22E-48 | -1.69E-46 |
| MS.gene060703 | MS.gene57034 | 0.910426 | 1.90E-82 | -1.69E-46 |
| MS.gene060728 | MS.gene57034 | 0.810826 | 9.50E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene57034 | 0.899154 | 2.62E-77 | -1.69E-46 |
| MS.gene060778 | MS.gene57034 | 0.82579 | 3.87E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene57034 | 0.88814 | 7.69E-73 | -1.69E-46 |
| MS.gene060823 | MS.gene57034 | -0.821511 | 3.88E-53 | -1.69E-46 |
| MS.gene06092 | MS.gene57034 | 0.818379 | 2.02E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene57034 | 0.862592 | 4.54E-64 | -1.69E-46 |
| MS.gene061134 | MS.gene57034 | 0.818612 | 1.79E-52 | -1.69E-46 |
| MS.gene061150 | MS.gene57034 | 0.90055 | 6.55E-78 | -1.69E-46 |
| MS.gene061171 | MS.gene57034 | 0.82205 | 2.91E-53 | -1.69E-46 |
| MS.gene061200 | MS.gene57034 | 0.821618 | 3.67E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCCTGTTTGATTTCTTTC+CGG | 0.253338 | 1.3:-24368506 | None:intergenic |
| ACTGCAATTTCTTTGTCTTA+TGG | 0.260657 | 1.3:-24368549 | None:intergenic |
| ATCAAGTCCATTCTTCTCTA+TGG | 0.282588 | 1.3:-24368656 | None:intergenic |
| AAGAGAGTTTGGTGGGAAGC+TGG | 0.407615 | 1.3:+24368589 | MS.gene57034:CDS |
| ACCTCCAGTACAAGAGAGTT+TGG | 0.413996 | 1.3:+24368578 | MS.gene57034:CDS |
| CTAGCAACAATTGGTTGAAA+AGG | 0.417846 | 1.3:-24366969 | None:intergenic |
| CTTTGTCTTATGGTTTGAAA+AGG | 0.427226 | 1.3:-24368539 | None:intergenic |
| GTTTGAAAAGGATACTTTGT+TGG | 0.430284 | 1.3:-24368527 | None:intergenic |
| AATAACTTACTAGCAACAAT+TGG | 0.433902 | 1.3:-24366978 | None:intergenic |
| GTTTGATTTCTTTCCGGTGA+AGG | 0.440508 | 1.3:-24368500 | None:intergenic |
| TCACCGGAAAGAAATCAAAC+AGG | 0.457839 | 1.3:+24368503 | MS.gene57034:CDS |
| AAAGCTTCGTTTGTCAACTA+AGG | 0.463346 | 1.3:+24368624 | MS.gene57034:CDS |
| TTCTTGAGTTCTCAGTTAAG+TGG | 0.484465 | 1.3:+24366891 | MS.gene57034:CDS |
| CCCACCAAACTCTCTTGTAC+TGG | 0.486040 | 1.3:-24368582 | None:intergenic |
| TGAAAAGGAGAAGCAACAAT+TGG | 0.492986 | 1.3:-24366954 | None:intergenic |
| TTTGATTTCTTTCCGGTGAA+GGG | 0.493506 | 1.3:-24368499 | None:intergenic |
| AGAGAGTTTGGTGGGAAGCT+GGG | 0.527154 | 1.3:+24368590 | MS.gene57034:CDS |
| TTGAAAACCATAGAGAAGAA+TGG | 0.536514 | 1.3:+24368649 | MS.gene57034:CDS |
| GAGAGAGTGTGTCCCTTCAC+CGG | 0.545165 | 1.3:+24368487 | MS.gene57034:intron |
| CCAGTACAAGAGAGTTTGGT+GGG | 0.583767 | 1.3:+24368582 | MS.gene57034:CDS |
| ACCAAACTCTCTTGTACTGG+AGG | 0.590235 | 1.3:-24368579 | None:intergenic |
| GGAGAAGCAACAATTGGTGA+GGG | 0.617414 | 1.3:-24366948 | None:intergenic |
| GTGGTCTCAGAATCTCGTCT+CGG | 0.624527 | 1.3:+24366910 | MS.gene57034:CDS |
| AGGAGAAGCAACAATTGGTG+AGG | 0.627644 | 1.3:-24366949 | None:intergenic |
| TCCAGTACAAGAGAGTTTGG+TGG | 0.651100 | 1.3:+24368581 | MS.gene57034:CDS |
| CACCGGAAAGAAATCAAACA+GGG | 0.654830 | 1.3:+24368504 | MS.gene57034:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATAAAAATAAAAAAGAA+CGG | - | chr1.3:24367099-24367118 | None:intergenic | 10.0% |
| !! | ATTTACAATAATAATAATCA+GGG | - | chr1.3:24368431-24368450 | None:intergenic | 10.0% |
| !!! | ATTTATTGAAGTTATTTTAA+TGG | + | chr1.3:24367513-24367532 | MS.gene57034:intron | 10.0% |
| !!! | TTATTTTTATTGTCATATTT+TGG | + | chr1.3:24367106-24367125 | MS.gene57034:intron | 10.0% |
| !! | AAGAAATGTTACAATAATTA+AGG | + | chr1.3:24367668-24367687 | MS.gene57034:intron | 15.0% |
| !! | CAAAAAAGAATTATTACTAT+AGG | - | chr1.3:24368056-24368075 | None:intergenic | 15.0% |
| !! | GATTTACAATAATAATAATC+AGG | - | chr1.3:24368432-24368451 | None:intergenic | 15.0% |
| !! | TATTGTAAATCAATTTAACT+TGG | + | chr1.3:24368441-24368460 | MS.gene57034:intron | 15.0% |
| !!! | AAAAAATAACATATTTCTGT+TGG | + | chr1.3:24367919-24367938 | MS.gene57034:intron | 15.0% |
| !!! | CTTTTATTTTCATAACAATA+GGG | + | chr1.3:24367693-24367712 | MS.gene57034:intron | 15.0% |
| !!! | TATTTTTTCGTATTTTATTG+CGG | + | chr1.3:24367950-24367969 | MS.gene57034:intron | 15.0% |
| !!! | TCCATTAAATAATAAGTTTT+TGG | + | chr1.3:24368301-24368320 | MS.gene57034:intron | 15.0% |
| !!! | TTGCTCTAATTTTAATAAAA+TGG | + | chr1.3:24367358-24367377 | MS.gene57034:intron | 15.0% |
| !! | AACATATGTTCTATACAAAT+AGG | - | chr1.3:24368182-24368201 | None:intergenic | 20.0% |
| !! | ATTTGTATAGAACATATGTT+GGG | + | chr1.3:24368182-24368201 | MS.gene57034:intron | 20.0% |
| !! | CCTATTGTTATGAAAATAAA+AGG | - | chr1.3:24367695-24367714 | None:intergenic | 20.0% |
| !! | GCCAAAAACTTATTATTTAA+TGG | - | chr1.3:24368305-24368324 | None:intergenic | 20.0% |
| !! | GTTTAACACTTATCATTTAA+GGG | - | chr1.3:24368218-24368237 | None:intergenic | 20.0% |
| !! | TATTTGTATAGAACATATGT+TGG | + | chr1.3:24368181-24368200 | MS.gene57034:intron | 20.0% |
| !! | TGTTTAACACTTATCATTTA+AGG | - | chr1.3:24368219-24368238 | None:intergenic | 20.0% |
| !!! | AACCAAATGTGTTTTTTTTT+TGG | - | chr1.3:24368355-24368374 | None:intergenic | 20.0% |
| !!! | ACCAAATGTGTTTTTTTTTT+GGG | - | chr1.3:24368354-24368373 | None:intergenic | 20.0% |
| !!! | CCTTTTATTTTCATAACAAT+AGG | + | chr1.3:24367692-24367711 | MS.gene57034:intron | 20.0% |
| !!! | GTAAACACTAGTAATAATTT+TGG | + | chr1.3:24367246-24367265 | MS.gene57034:intron | 20.0% |
| ! | AAGGAATTATTGCATAAAGA+CGG | - | chr1.3:24367572-24367591 | None:intergenic | 25.0% |
| ! | AATAACTTACTAGCAACAAT+TGG | - | chr1.3:24366981-24367000 | None:intergenic | 25.0% |
| ! | ACCCAAAAAAAAAACACATT+TGG | + | chr1.3:24368350-24368369 | MS.gene57034:intron | 25.0% |
| ! | CTATTAGTTAGAGATATCAT+GGG | + | chr1.3:24368324-24368343 | MS.gene57034:intron | 25.0% |
| ! | TAACACATCTACAAAAAACA+AGG | - | chr1.3:24367591-24367610 | None:intergenic | 25.0% |
| ! | TGCTTAAGAAACTCAATTAT+AGG | - | chr1.3:24368127-24368146 | None:intergenic | 25.0% |
| !! | ATTTTCATAACAATAGGGAT+AGG | + | chr1.3:24367698-24367717 | MS.gene57034:intron | 25.0% |
| !! | TGATTTTGTCAAGTTATTAG+TGG | - | chr1.3:24367797-24367816 | None:intergenic | 25.0% |
| !! | TTCAATCTGAAGTATATTAG+AGG | + | chr1.3:24367639-24367658 | MS.gene57034:intron | 25.0% |
| AACTACATCATTAGTGTCTA+AGG | + | chr1.3:24368392-24368411 | MS.gene57034:intron | 30.0% | |
| AAGTGTTAAACATGCTGTTA+GGG | + | chr1.3:24368227-24368246 | MS.gene57034:intron | 30.0% | |
| ACTATAGGCAAACATAAACT+AGG | - | chr1.3:24368041-24368060 | None:intergenic | 30.0% | |
| ACTGCAATTTCTTTGTCTTA+TGG | - | chr1.3:24368552-24368571 | None:intergenic | 30.0% | |
| ATAAGAGAACATGCATAACA+TGG | + | chr1.3:24368087-24368106 | MS.gene57034:intron | 30.0% | |
| ATATGTCCTTCAGTAAAAAC+AGG | - | chr1.3:24368266-24368285 | None:intergenic | 30.0% | |
| CTTTGTCTTATGGTTTGAAA+AGG | - | chr1.3:24368542-24368561 | None:intergenic | 30.0% | |
| GCTATTAGTTAGAGATATCA+TGG | + | chr1.3:24368323-24368342 | MS.gene57034:intron | 30.0% | |
| GTTTGAAAAGGATACTTTGT+TGG | - | chr1.3:24368530-24368549 | None:intergenic | 30.0% | |
| TAAGTGTTAAACATGCTGTT+AGG | + | chr1.3:24368226-24368245 | MS.gene57034:intron | 30.0% | |
| TAGACACTAATGATGTAGTT+TGG | - | chr1.3:24368392-24368411 | None:intergenic | 30.0% | |
| TTGAAAACCATAGAGAAGAA+TGG | + | chr1.3:24368649-24368668 | MS.gene57034:CDS | 30.0% | |
| TTGTATATCGCAATCAGATA+AGG | - | chr1.3:24367143-24367162 | None:intergenic | 30.0% | |
| ! | TCAGAGTGAATATCGATTAT+TGG | - | chr1.3:24367008-24367027 | None:intergenic | 30.0% |
| ! | TCTGAAGTATATTAGAGGAT+AGG | + | chr1.3:24367644-24367663 | MS.gene57034:intron | 30.0% |
| !! | CAATTTAACTTGGTGTACTT+TGG | + | chr1.3:24368451-24368470 | MS.gene57034:intron | 30.0% |
| !!! | ATAAGACCTGTTTTTACTGA+AGG | + | chr1.3:24368257-24368276 | MS.gene57034:intron | 30.0% |
| AAAGCTTCGTTTGTCAACTA+AGG | + | chr1.3:24368624-24368643 | MS.gene57034:CDS | 35.0% | |
| ACTTGATGCAGTTGCAAAAA+AGG | + | chr1.3:24368672-24368691 | MS.gene57034:CDS | 35.0% | |
| AGGCAAACATAAACTAGGAA+GGG | - | chr1.3:24368036-24368055 | None:intergenic | 35.0% | |
| ATCAAGTCCATTCTTCTCTA+TGG | - | chr1.3:24368659-24368678 | None:intergenic | 35.0% | |
| ATGCTTCACTATTCACTTAC+AGG | - | chr1.3:24367737-24367756 | None:intergenic | 35.0% | |
| CATAACAATAGGGATAGGAA+AGG | + | chr1.3:24367703-24367722 | MS.gene57034:intron | 35.0% | |
| CCATAGTAAGCTATTAAGCA+CGG | - | chr1.3:24367878-24367897 | None:intergenic | 35.0% | |
| CTAGCAACAATTGGTTGAAA+AGG | - | chr1.3:24366972-24366991 | None:intergenic | 35.0% | |
| TAGGCAAACATAAACTAGGA+AGG | - | chr1.3:24368037-24368056 | None:intergenic | 35.0% | |
| TGAAAAGGAGAAGCAACAAT+TGG | - | chr1.3:24366957-24366976 | None:intergenic | 35.0% | |
| TGTAAGTGAATAGTGAAGCA+TGG | + | chr1.3:24367736-24367755 | MS.gene57034:intron | 35.0% | |
| TGTCAAGTTATTAGTGGTGT+CGG | - | chr1.3:24367791-24367810 | None:intergenic | 35.0% | |
| TTCTTGAGTTCTCAGTTAAG+TGG | + | chr1.3:24366891-24366910 | MS.gene57034:CDS | 35.0% | |
| ! | CTACATACTTTGTTTCTTGC+AGG | + | chr1.3:24367470-24367489 | MS.gene57034:intron | 35.0% |
| ! | TTTGATTTCTTTCCGGTGAA+GGG | - | chr1.3:24368502-24368521 | None:intergenic | 35.0% |
| AATTATATGTGTCCGACACC+GGG | + | chr1.3:24367830-24367849 | MS.gene57034:intron | 40.0% | |
| AGAACATGCATAACATGGTG+TGG | + | chr1.3:24368092-24368111 | MS.gene57034:intron | 40.0% | |
| CAATTATATGTGTCCGACAC+CGG | + | chr1.3:24367829-24367848 | MS.gene57034:intron | 40.0% | |
| CACCGGAAAGAAATCAAACA+GGG | + | chr1.3:24368504-24368523 | MS.gene57034:CDS | 40.0% | |
| CATGTCATTGTCAGTTACCA+TGG | - | chr1.3:24367227-24367246 | None:intergenic | 40.0% | |
| CCGTGCTTAATAGCTTACTA+TGG | + | chr1.3:24367875-24367894 | MS.gene57034:intron | 40.0% | |
| CTGATACGTGTTAGAGACAA+CGG | + | chr1.3:24367420-24367439 | MS.gene57034:intron | 40.0% | |
| TCACCGGAAAGAAATCAAAC+AGG | + | chr1.3:24368503-24368522 | MS.gene57034:CDS | 40.0% | |
| TGATACGTGTTAGAGACAAC+GGG | + | chr1.3:24367421-24367440 | MS.gene57034:intron | 40.0% | |
| ! | GTTTGATTTCTTTCCGGTGA+AGG | - | chr1.3:24368503-24368522 | None:intergenic | 40.0% |
| ! | TCGTATTTTATTGCGGACAG+AGG | + | chr1.3:24367957-24367976 | MS.gene57034:intron | 40.0% |
| ACCAAACTCTCTTGTACTGG+AGG | - | chr1.3:24368582-24368601 | None:intergenic | 45.0% | |
| ACCTCCAGTACAAGAGAGTT+TGG | + | chr1.3:24368578-24368597 | MS.gene57034:CDS | 45.0% | |
| AGGAGAAGCAACAATTGGTG+AGG | - | chr1.3:24366952-24366971 | None:intergenic | 45.0% | |
| CACAAGTCAGTGTCAAACAC+AGG | - | chr1.3:24367307-24367326 | None:intergenic | 45.0% | |
| CCAGTACAAGAGAGTTTGGT+GGG | + | chr1.3:24368582-24368601 | MS.gene57034:CDS | 45.0% | |
| GAATAGTGAAGCATGGACAC+CGG | + | chr1.3:24367743-24367762 | MS.gene57034:intron | 45.0% | |
| GATGCAGTTGCAAAAAAGGC+TGG | + | chr1.3:24368676-24368695 | MS.gene57034:CDS | 45.0% | |
| GGAGAAGCAACAATTGGTGA+GGG | - | chr1.3:24366951-24366970 | None:intergenic | 45.0% | |
| GTCACTCAAACGATGAAAGC+TGG | - | chr1.3:24368004-24368023 | None:intergenic | 45.0% | |
| TATTAAGCACGGACACTCCT+AGG | - | chr1.3:24367867-24367886 | None:intergenic | 45.0% | |
| TCCAGTACAAGAGAGTTTGG+TGG | + | chr1.3:24368581-24368600 | MS.gene57034:CDS | 45.0% | |
| ! | CTGTTGCTAGTTTACGTCCA+TGG | + | chr1.3:24367207-24367226 | MS.gene57034:intron | 45.0% |
| ! | GGCCCTGTTTGATTTCTTTC+CGG | - | chr1.3:24368509-24368528 | None:intergenic | 45.0% |
| CCCACCAAACTCTCTTGTAC+TGG | - | chr1.3:24368585-24368604 | None:intergenic | 50.0% | |
| GTGGTCTCAGAATCTCGTCT+CGG | + | chr1.3:24366910-24366929 | MS.gene57034:CDS | 50.0% | |
| ! | AAGAGAGTTTGGTGGGAAGC+TGG | + | chr1.3:24368589-24368608 | MS.gene57034:CDS | 50.0% |
| ! | AGAGAGTTTGGTGGGAAGCT+GGG | + | chr1.3:24368590-24368609 | MS.gene57034:CDS | 50.0% |
| GCACGGACACTCCTAGGATT+AGG | - | chr1.3:24367861-24367880 | None:intergenic | 55.0% | |
| GTTAGTGTCCGTGTCGTGTC+CGG | - | chr1.3:24367765-24367784 | None:intergenic | 55.0% | |
| TCCTAGGATTAGGCGTGTCC+CGG | - | chr1.3:24367851-24367870 | None:intergenic | 55.0% | |
| ! | GAGAGAGTGTGTCCCTTCAC+CGG | + | chr1.3:24368487-24368506 | MS.gene57034:intron | 55.0% |
| ACCGGGACACGCCTAATCCT+AGG | + | chr1.3:24367847-24367866 | MS.gene57034:intron | 60.0% | |
| CATGGACACCGGACACGACA+CGG | + | chr1.3:24367754-24367773 | MS.gene57034:intron | 60.0% | |
| GATTAGGCGTGTCCCGGTGT+CGG | - | chr1.3:24367845-24367864 | None:intergenic | 60.0% | |
| GCTTTGACGCGCAGGCTGTG+TGG | + | chr1.3:24367175-24367194 | MS.gene57034:intron | 65.0% | |
| CGCGCAGGCTGTGTGGAGCA+CGG | + | chr1.3:24367182-24367201 | MS.gene57034:intron | 70.0% | |
| CTGTGCGCGCTTTGACGCGC+AGG | + | chr1.3:24367167-24367186 | MS.gene57034:intron | 70.0% | |
| GCGCAGGCTGTGTGGAGCAC+GGG | + | chr1.3:24367183-24367202 | MS.gene57034:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 24366883 | 24368720 | 24366883 | ID=MS.gene57034 |
| chr1.3 | mRNA | 24366883 | 24368720 | 24366883 | ID=MS.gene57034.t1;Parent=MS.gene57034 |
| chr1.3 | exon | 24366883 | 24366990 | 24366883 | ID=MS.gene57034.t1.exon1;Parent=MS.gene57034.t1 |
| chr1.3 | CDS | 24366883 | 24366990 | 24366883 | ID=cds.MS.gene57034.t1;Parent=MS.gene57034.t1 |
| chr1.3 | exon | 24368488 | 24368720 | 24368488 | ID=MS.gene57034.t1.exon2;Parent=MS.gene57034.t1 |
| chr1.3 | CDS | 24368488 | 24368720 | 24368488 | ID=cds.MS.gene57034.t1;Parent=MS.gene57034.t1 |
| Gene Sequence |
| Protein sequence |
