AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0649350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0649350 MtrunA17_Chr4g0034371 100.000 66 0 0 1 66 479 544 1.01e-39 139
Msa0649350 MtrunA17_Chr4g0034361 98.148 54 1 0 68 121 1 54 3.60e-33 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0649350 AT4G34370.2 47.059 136 48 6 1 121 304 430 1.49e-27 105
Msa0649350 AT4G34370.1 47.059 136 48 6 1 121 471 597 3.12e-27 105
Msa0649350 AT2G16090.2 40.945 127 62 3 1 120 412 532 3.18e-25 99.4
Msa0649350 AT2G16090.1 40.945 127 62 3 1 120 472 592 3.32e-25 99.4
Msa0649350 AT2G16090.4 42.857 133 45 5 1 120 472 586 2.82e-23 94.0
Msa0649350 AT2G16090.5 58.182 55 22 1 1 55 412 465 2.45e-16 73.9
Msa0649350 AT2G16090.3 58.182 55 22 1 1 55 472 525 2.61e-16 73.9
Msa0649350 AT3G27710.1 52.727 55 25 1 1 55 471 524 4.68e-14 67.4
Msa0649350 AT3G27720.1 49.091 55 27 1 1 55 431 484 4.79e-13 64.7

Find 16 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCATCGACAATGATTTAT+TGG 0.189539 4_3:+55980037 Msa0649350:CDS
TCTATGCCCTTTGATTTATA+TGG 0.230617 4_3:-55980095 None:intergenic
CTTCAGAGCTTCCAGTTTGT+TGG 0.314668 4_3:+55980123 Msa0649350:CDS
GCTTTATTGCTCCAACAAAC+TGG 0.371482 4_3:-55980134 None:intergenic
AGAAATGTTACTGAGTGAAA+AGG 0.489380 4_3:+55980804 Msa0649350:intron
GAGCAATAAAGCTGACAATA+CGG 0.496778 4_3:+55980145 Msa0649350:CDS
CGTAAGCGTGCCAGAGAAGA+AGG 0.497557 4_3:+55980901 Msa0649350:CDS
TATAGCACCATATAAATCAA+AGG 0.523492 4_3:+55980088 Msa0649350:CDS
ATAGCACCATATAAATCAAA+GGG 0.529766 4_3:+55980089 Msa0649350:CDS
TTTCTGTCTACAACTTCAGC+TGG 0.532183 4_3:-55980946 None:intergenic
TTATTGGATTCTATTAATCT+CGG 0.532615 4_3:+55980053 Msa0649350:CDS
AGCAATAAAGCTGACAATAC+GGG 0.541090 4_3:+55980146 Msa0649350:CDS
TGTATATATGCTGCAAGCGT+AGG 0.573507 4_3:+55980832 Msa0649350:CDS
CTTAATCAGTCAGAAGAGAG+TGG 0.594834 4_3:+55980868 Msa0649350:CDS
ATTTATATGGTGCTATACTG+TGG 0.634097 4_3:-55980082 None:intergenic
AAGCGTGCCAGAGAAGAAGG+TGG 0.717667 4_3:+55980904 Msa0649350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTGGATTCTATTAATCT+CGG + chr4_3:55980053-55980072 Msa0649350:CDS 20.0%
!!! AAGTAATGGAAAATTAATCA+TGG - chr4_3:55980432-55980451 None:intergenic 20.0%
!! TTAATAAAACCACTGTTAAT+TGG + chr4_3:55980532-55980551 Msa0649350:intron 20.0%
!! TAATAAAACCACTGTTAATT+GGG + chr4_3:55980533-55980552 Msa0649350:intron 20.0%
!! GCTCAAGATATAAATTAATT+TGG - chr4_3:55980714-55980733 None:intergenic 20.0%
!! TTATTGGATTCTATTAATCT+CGG + chr4_3:55980053-55980072 Msa0649350:CDS 20.0%
!!! AAGTAATGGAAAATTAATCA+TGG - chr4_3:55980432-55980451 None:intergenic 20.0%
!! TTAATAAAACCACTGTTAAT+TGG + chr4_3:55980532-55980551 Msa0649350:intron 20.0%
!! TAATAAAACCACTGTTAATT+GGG + chr4_3:55980533-55980552 Msa0649350:intron 20.0%
!! GCTCAAGATATAAATTAATT+TGG - chr4_3:55980714-55980733 None:intergenic 20.0%
! TGAATTCTTATATTGAATGC+AGG + chr4_3:55980009-55980028 Msa0649350:intron 25.0%
! TATAGCACCATATAAATCAA+AGG + chr4_3:55980088-55980107 Msa0649350:CDS 25.0%
! ATAGCACCATATAAATCAAA+GGG + chr4_3:55980089-55980108 Msa0649350:CDS 25.0%
! TAAATGTCTATTGTTGGATT+TGG + chr4_3:55980169-55980188 Msa0649350:intron 25.0%
! TATATGCACAATACAAGTAA+TGG - chr4_3:55980446-55980465 None:intergenic 25.0%
!!! CAGTGGTTTTATTAAAATCA+GGG - chr4_3:55980527-55980546 None:intergenic 25.0%
!!! ACAGTGGTTTTATTAAAATC+AGG - chr4_3:55980528-55980547 None:intergenic 25.0%
! TTTCATGATTGAATTGTGTA+TGG + chr4_3:55980567-55980586 Msa0649350:intron 25.0%
! TGAATTCTTATATTGAATGC+AGG + chr4_3:55980009-55980028 Msa0649350:intron 25.0%
! TATAGCACCATATAAATCAA+AGG + chr4_3:55980088-55980107 Msa0649350:CDS 25.0%
! ATAGCACCATATAAATCAAA+GGG + chr4_3:55980089-55980108 Msa0649350:CDS 25.0%
! TAAATGTCTATTGTTGGATT+TGG + chr4_3:55980169-55980188 Msa0649350:intron 25.0%
! TATATGCACAATACAAGTAA+TGG - chr4_3:55980446-55980465 None:intergenic 25.0%
!!! CAGTGGTTTTATTAAAATCA+GGG - chr4_3:55980527-55980546 None:intergenic 25.0%
!!! ACAGTGGTTTTATTAAAATC+AGG - chr4_3:55980528-55980547 None:intergenic 25.0%
! TTTCATGATTGAATTGTGTA+TGG + chr4_3:55980567-55980586 Msa0649350:intron 25.0%
!! ATTTATATGGTGCTATACTG+TGG - chr4_3:55980085-55980104 None:intergenic 30.0%
! TCTATGCCCTTTGATTTATA+TGG - chr4_3:55980098-55980117 None:intergenic 30.0%
ACCTGTTATCAGTAACTAAT+TGG + chr4_3:55980283-55980302 Msa0649350:intron 30.0%
CCTGTTATCAGTAACTAATT+GGG + chr4_3:55980284-55980303 Msa0649350:intron 30.0%
CAGTAACTAATTGGGTTATA+TGG + chr4_3:55980292-55980311 Msa0649350:intron 30.0%
ACTTGTATTGTGCATATATG+TGG + chr4_3:55980448-55980467 Msa0649350:intron 30.0%
CTTGTATTGTGCATATATGT+GGG + chr4_3:55980449-55980468 Msa0649350:intron 30.0%
TTAAAATCAGGGTGAAATCA+AGG - chr4_3:55980516-55980535 None:intergenic 30.0%
TGAATGAATATATTGCATCG+TGG + chr4_3:55980593-55980612 Msa0649350:intron 30.0%
GTTGTTGTAACATGAAAAAG+TGG + chr4_3:55980686-55980705 Msa0649350:intron 30.0%
!! TTTTCTTCAGTTTGACTTTG+TGG + chr4_3:55980775-55980794 Msa0649350:intron 30.0%
AGAAATGTTACTGAGTGAAA+AGG + chr4_3:55980804-55980823 Msa0649350:intron 30.0%
!! ATTTATATGGTGCTATACTG+TGG - chr4_3:55980085-55980104 None:intergenic 30.0%
! TCTATGCCCTTTGATTTATA+TGG - chr4_3:55980098-55980117 None:intergenic 30.0%
ACCTGTTATCAGTAACTAAT+TGG + chr4_3:55980283-55980302 Msa0649350:intron 30.0%
CCTGTTATCAGTAACTAATT+GGG + chr4_3:55980284-55980303 Msa0649350:intron 30.0%
CAGTAACTAATTGGGTTATA+TGG + chr4_3:55980292-55980311 Msa0649350:intron 30.0%
ACTTGTATTGTGCATATATG+TGG + chr4_3:55980448-55980467 Msa0649350:intron 30.0%
CTTGTATTGTGCATATATGT+GGG + chr4_3:55980449-55980468 Msa0649350:intron 30.0%
TTAAAATCAGGGTGAAATCA+AGG - chr4_3:55980516-55980535 None:intergenic 30.0%
TGAATGAATATATTGCATCG+TGG + chr4_3:55980593-55980612 Msa0649350:intron 30.0%
GTTGTTGTAACATGAAAAAG+TGG + chr4_3:55980686-55980705 Msa0649350:intron 30.0%
!! TTTTCTTCAGTTTGACTTTG+TGG + chr4_3:55980775-55980794 Msa0649350:intron 30.0%
AGAAATGTTACTGAGTGAAA+AGG + chr4_3:55980804-55980823 Msa0649350:intron 30.0%
! TAAGTGTTGTGCTTTAGATG+AGG + chr4_3:55979950-55979969 Msa0649350:intron 35.0%
! GTGCATCGACAATGATTTAT+TGG + chr4_3:55980037-55980056 Msa0649350:CDS 35.0%
GAGCAATAAAGCTGACAATA+CGG + chr4_3:55980145-55980164 Msa0649350:CDS 35.0%
AGCAATAAAGCTGACAATAC+GGG + chr4_3:55980146-55980165 Msa0649350:CDS 35.0%
TACGGGTAAATGTCTATTGT+TGG + chr4_3:55980163-55980182 Msa0649350:intron 35.0%
CCCAATTAGTTACTGATAAC+AGG - chr4_3:55980287-55980306 None:intergenic 35.0%
AAAACCACTGTTAATTGGGA+TGG + chr4_3:55980537-55980556 Msa0649350:intron 35.0%
AAATCCATCCCAATTAACAG+TGG - chr4_3:55980544-55980563 None:intergenic 35.0%
! TAAGTGTTGTGCTTTAGATG+AGG + chr4_3:55979950-55979969 Msa0649350:intron 35.0%
! GTGCATCGACAATGATTTAT+TGG + chr4_3:55980037-55980056 Msa0649350:CDS 35.0%
GAGCAATAAAGCTGACAATA+CGG + chr4_3:55980145-55980164 Msa0649350:CDS 35.0%
AGCAATAAAGCTGACAATAC+GGG + chr4_3:55980146-55980165 Msa0649350:CDS 35.0%
TACGGGTAAATGTCTATTGT+TGG + chr4_3:55980163-55980182 Msa0649350:intron 35.0%
CCCAATTAGTTACTGATAAC+AGG - chr4_3:55980287-55980306 None:intergenic 35.0%
AAAACCACTGTTAATTGGGA+TGG + chr4_3:55980537-55980556 Msa0649350:intron 35.0%
AAATCCATCCCAATTAACAG+TGG - chr4_3:55980544-55980563 None:intergenic 35.0%
! GCTTTATTGCTCCAACAAAC+TGG - chr4_3:55980137-55980156 None:intergenic 40.0%
GAAGCAACGGAATAATACGA+GGG - chr4_3:55980221-55980240 None:intergenic 40.0%
AGAAGCAACGGAATAATACG+AGG - chr4_3:55980222-55980241 None:intergenic 40.0%
CAGCATAAAGAGAGAAGCAA+CGG - chr4_3:55980234-55980253 None:intergenic 40.0%
GTCTCAACATGTACACTTTG+TGG + chr4_3:55980348-55980367 Msa0649350:intron 40.0%
TGTACACTTTGTGGTCATGT+TGG + chr4_3:55980357-55980376 Msa0649350:intron 40.0%
TGTGGGAGATCATAGTTGTA+TGG + chr4_3:55980466-55980485 Msa0649350:intron 40.0%
GTGGGAGATCATAGTTGTAT+GGG + chr4_3:55980467-55980486 Msa0649350:intron 40.0%
TGGGAGATCATAGTTGTATG+GGG + chr4_3:55980468-55980487 Msa0649350:intron 40.0%
GATCATAGTTGTATGGGGAT+CGG + chr4_3:55980473-55980492 Msa0649350:intron 40.0%
TGTATATATGCTGCAAGCGT+AGG + chr4_3:55980832-55980851 Msa0649350:CDS 40.0%
CTTAATCAGTCAGAAGAGAG+TGG + chr4_3:55980868-55980887 Msa0649350:CDS 40.0%
AAAAAAGCCACCTTCTTCTC+TGG - chr4_3:55980914-55980933 None:intergenic 40.0%
TTTCTGTCTACAACTTCAGC+TGG - chr4_3:55980949-55980968 None:intergenic 40.0%
! GCTTTATTGCTCCAACAAAC+TGG - chr4_3:55980137-55980156 None:intergenic 40.0%
GAAGCAACGGAATAATACGA+GGG - chr4_3:55980221-55980240 None:intergenic 40.0%
AGAAGCAACGGAATAATACG+AGG - chr4_3:55980222-55980241 None:intergenic 40.0%
CAGCATAAAGAGAGAAGCAA+CGG - chr4_3:55980234-55980253 None:intergenic 40.0%
GTCTCAACATGTACACTTTG+TGG + chr4_3:55980348-55980367 Msa0649350:intron 40.0%
TGTACACTTTGTGGTCATGT+TGG + chr4_3:55980357-55980376 Msa0649350:intron 40.0%
TGTGGGAGATCATAGTTGTA+TGG + chr4_3:55980466-55980485 Msa0649350:intron 40.0%
GTGGGAGATCATAGTTGTAT+GGG + chr4_3:55980467-55980486 Msa0649350:intron 40.0%
TGGGAGATCATAGTTGTATG+GGG + chr4_3:55980468-55980487 Msa0649350:intron 40.0%
GATCATAGTTGTATGGGGAT+CGG + chr4_3:55980473-55980492 Msa0649350:intron 40.0%
TGTATATATGCTGCAAGCGT+AGG + chr4_3:55980832-55980851 Msa0649350:CDS 40.0%
CTTAATCAGTCAGAAGAGAG+TGG + chr4_3:55980868-55980887 Msa0649350:CDS 40.0%
AAAAAAGCCACCTTCTTCTC+TGG - chr4_3:55980914-55980933 None:intergenic 40.0%
TTTCTGTCTACAACTTCAGC+TGG - chr4_3:55980949-55980968 None:intergenic 40.0%
CTTCAGAGCTTCCAGTTTGT+TGG + chr4_3:55980123-55980142 Msa0649350:CDS 45.0%
GGAATAATACGAGGGAGTTG+CGG - chr4_3:55980213-55980232 None:intergenic 45.0%
!!! GTATGGGGATCGGTTAGTTT+TGG + chr4_3:55980483-55980502 Msa0649350:intron 45.0%
CTTCAGAGCTTCCAGTTTGT+TGG + chr4_3:55980123-55980142 Msa0649350:CDS 45.0%
GGAATAATACGAGGGAGTTG+CGG - chr4_3:55980213-55980232 None:intergenic 45.0%
!!! GTATGGGGATCGGTTAGTTT+TGG + chr4_3:55980483-55980502 Msa0649350:intron 45.0%
CGTAAGCGTGCCAGAGAAGA+AGG + chr4_3:55980901-55980920 Msa0649350:CDS 55.0%
AAGCGTGCCAGAGAAGAAGG+TGG + chr4_3:55980904-55980923 Msa0649350:CDS 55.0%
CGTAAGCGTGCCAGAGAAGA+AGG + chr4_3:55980901-55980920 Msa0649350:CDS 55.0%
AAGCGTGCCAGAGAAGAAGG+TGG + chr4_3:55980904-55980923 Msa0649350:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 55979886 55980972 55979886 ID=Msa0649350;Name=Msa0649350
chr4_3 mRNA 55979886 55980972 55979886 ID=Msa0649350-mRNA-1;Parent=Msa0649350;Name=Msa0649350-mRNA-1;_AED=0.38;_eAED=0.38;_QI=1|0.5|0.66|1|1|1|3|0|121
chr4_3 exon 55979886 55979948 55979886 ID=Msa0649350-mRNA-1:exon:14431;Parent=Msa0649350-mRNA-1
chr4_3 exon 55980031 55980167 55980031 ID=Msa0649350-mRNA-1:exon:14432;Parent=Msa0649350-mRNA-1
chr4_3 exon 55980806 55980972 55980806 ID=Msa0649350-mRNA-1:exon:14433;Parent=Msa0649350-mRNA-1
chr4_3 five_prime_UTR 55979886 55979886 55979886 ID=Msa0649350-mRNA-1:five_prime_utr;Parent=Msa0649350-mRNA-1
chr4_3 CDS 55979887 55979948 55979887 ID=Msa0649350-mRNA-1:cds;Parent=Msa0649350-mRNA-1
chr4_3 CDS 55980031 55980167 55980031 ID=Msa0649350-mRNA-1:cds;Parent=Msa0649350-mRNA-1
chr4_3 CDS 55980806 55980972 55980806 ID=Msa0649350-mRNA-1:cds;Parent=Msa0649350-mRNA-1
Gene Sequence

>Msa0649350

ATGGAGATAAGGATGCAGATAATAACTCTGTCTGCACTCATTGATGATCTTTGTAAAAAAATGTATGAGTGCATCGACAATGATTTATTGGATTCTATTAATCTCGGTAGTGTCCACAGTATAGCACCATATAAATCAAAGGGCATAGAGAAAGCTTCAGAGCTTCCAGTTTGTTGGAGCAATAAAGCTGACAATACGGAAATGTTACTGAGTGAAAAGGATTCTTGTATATATGCTGCAAGCGTAGGTGTAACAGCAGAGCTTAATCAGTCAGAAGAGAGTGGATGTTCTTCTCGTAAGCGTGCCAGAGAAGAAGGTGGCTTTTTTGATCTAAACTTGCCAGCTGAAGTTGTAGACAGAAATTGA

Protein sequence

>Msa0649350

MEIRMQIITLSALIDDLCKKMYECIDNDLLDSINLGSVHSIAPYKSKGIEKASELPVCWSNKADNTEMLLSEKDSCIYAASVGVTAELNQSEESGCSSRKRAREEGGFFDLNLPAEVVDRN*