AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0812290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0812290 MtrunA17_Chr4g0020281 100.000 48 0 0 1 48 1 48 5.59e-29 97.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0812290 AT3G52040.1 72.917 48 13 0 1 48 1 48 1.37e-17 69.3

Find 45 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTATGAATCTCAGAATT+TGG 0.189259 5_3:+42242633 None:intergenic
TCCTCCCGTTGTAGTGTTAA+TGG 0.192394 5_3:+42242712 None:intergenic
GAAATGAAAGAATTAGAATT+AGG 0.193966 5_3:+42243815 None:intergenic
GGCATAGAGGGTTGGTTTAA+TGG 0.195375 5_3:-42242581 Msa0812290:three_prime_UTR
TAATCAAGTGGTTGCCAAAA+AGG 0.245471 5_3:+42242449 None:intergenic
AGAATAAAGAGTTAATTTAA+TGG 0.256489 5_3:+42242140 None:intergenic
AATACTAATTATAGAGAAAT+AGG 0.269817 5_3:-42242675 Msa0812290:three_prime_UTR
AAAGAATTAGAATTAGGGTT+AGG 0.316368 5_3:+42243821 None:intergenic
AAATGAAAGAATTAGAATTA+GGG 0.317434 5_3:+42243816 None:intergenic
AACGAAAGTTACAAAGGATA+TGG 0.347960 5_3:-42243580 Msa0812290:CDS
GAATCGATTTCTTCTTAGTT+TGG 0.349646 5_3:+42243745 None:intergenic
GCATTGATGAAAAGCGTTAT+TGG 0.368832 5_3:-42242077 Msa0812290:three_prime_UTR
TCGATTTCGAAATATACAAT+TGG 0.376395 5_3:+42243861 None:intergenic
GAGGGTTGGTTTAATGGCAT+TGG 0.381497 5_3:-42242575 Msa0812290:three_prime_UTR
AATTTACCTTTGCGAGTTTG+AGG 0.415610 5_3:+42243699 None:intergenic
CGATTTCGAAATATACAATT+GGG 0.415895 5_3:+42243862 None:intergenic
AAGAATTAGAATTAGGGTTA+GGG 0.422423 5_3:+42243822 None:intergenic
ATATCCTTTGTAACTTTCGT+TGG 0.460250 5_3:+42243582 None:intergenic
AAAGCTAAGAAATGTGTGTA+TGG 0.464251 5_3:-42242342 Msa0812290:three_prime_UTR
GAAGCCATTAACACTACAAC+GGG 0.464801 5_3:-42242716 Msa0812290:three_prime_UTR
TGGTCATGATATTTGATGAT+TGG 0.472708 5_3:-42242280 Msa0812290:three_prime_UTR
ATGTGTGTATGGACTGTTAC+GGG 0.512971 5_3:-42242331 Msa0812290:three_prime_UTR
ACTTTGCCATGACGATTAGC+AGG 0.529511 5_3:+42243723 None:intergenic
TTAAACCAACCCTCTATGCC+TGG 0.533815 5_3:+42242584 None:intergenic
TTGAGGACACCAGGCATAGA+GGG 0.536884 5_3:-42242593 Msa0812290:three_prime_UTR
GGACACCAGGCATAGAGGGT+TGG 0.554857 5_3:-42242589 Msa0812290:three_prime_UTR
AGGATCAGTTGAACATCTGT+CGG 0.555154 5_3:+42242506 None:intergenic
GTAACTTGTAAGCACTTGTA+CGG 0.563547 5_3:-42242741 Msa0812290:exon
GGATCAGTTGAACATCTGTC+GGG 0.563586 5_3:+42242507 None:intergenic
ATTGTCACATGGGAGTAGAT+AGG 0.566342 5_3:-42242403 Msa0812290:three_prime_UTR
TTTGAGGACACCAGGCATAG+AGG 0.578341 5_3:-42242594 Msa0812290:three_prime_UTR
AGATTCATAGAACGAAGCTA+AGG 0.585176 5_3:-42242623 Msa0812290:three_prime_UTR
TCGATTCCTGCTAATCGTCA+TGG 0.587351 5_3:-42243729 Msa0812290:CDS
GATTCATAGAACGAAGCTAA+GGG 0.611997 5_3:-42242622 Msa0812290:three_prime_UTR
AAAGTACCTCAAACTCGCAA+AGG 0.619602 5_3:-42243705 Msa0812290:intron
GCCATTAACACTACAACGGG+AGG 0.622819 5_3:-42242713 Msa0812290:three_prime_UTR
ACTTGTAAGCACTTGTACGG+TGG 0.626055 5_3:-42242738 Msa0812290:three_prime_UTR
AGATGTTCAACTGATCCTCT+CGG 0.627027 5_3:-42242501 Msa0812290:three_prime_UTR
TATCTGCAGAAGAAGACTGA+GGG 0.633770 5_3:-42242533 Msa0812290:three_prime_UTR
AAGCAAGGAGAATTGTCACA+TGG 0.643229 5_3:-42242414 Msa0812290:three_prime_UTR
AGCAAGGAGAATTGTCACAT+GGG 0.662171 5_3:-42242413 Msa0812290:three_prime_UTR
AATGTGTGTATGGACTGTTA+CGG 0.666756 5_3:-42242332 Msa0812290:three_prime_UTR
GGAAGCCATTAACACTACAA+CGG 0.667610 5_3:-42242717 Msa0812290:three_prime_UTR
GAAGCCAACGAAAGTTACAA+AGG 0.673022 5_3:-42243586 Msa0812290:CDS
ATATCTGCAGAAGAAGACTG+AGG 0.682318 5_3:-42242534 Msa0812290:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAACAATAAAAAAAATAA+TGG + chr5_3:42242263-42242282 None:intergenic 10.0%
!! ATACAATTATCTAATTTAAT+TGG - chr5_3:42243040-42243059 Msa0812290:intron 10.0%
!! TTCAACAATAAAAAAAATAA+TGG + chr5_3:42242263-42242282 None:intergenic 10.0%
!! ATACAATTATCTAATTTAAT+TGG - chr5_3:42243040-42243059 Msa0812290:intron 10.0%
!! AAATGAAAGAATTAGAATTA+GGG + chr5_3:42242117-42242136 None:intergenic 15.0%
!!! TTTAATTTTCAGTTATGATT+TGG - chr5_3:42242448-42242467 Msa0812290:three_prime_UTR 15.0%
!!! TTAATTTTCAGTTATGATTT+GGG - chr5_3:42242449-42242468 Msa0812290:three_prime_UTR 15.0%
!! TATATTAACAGTTTAAACAA+TGG - chr5_3:42243115-42243134 Msa0812290:intron 15.0%
!! AATACTAATTATAGAGAAAT+AGG - chr5_3:42243255-42243274 Msa0812290:intron 15.0%
!!! GAATTTTTTTTTTAATCAAG+TGG + chr5_3:42243496-42243515 None:intergenic 15.0%
!! TTAAAAAAAAAATTCAAGCA+AGG - chr5_3:42243501-42243520 Msa0812290:intron 15.0%
!! AGAATAAAGAGTTAATTTAA+TGG + chr5_3:42243793-42243812 None:intergenic 15.0%
!!! TTTATTCTCTTAAATTTTGT+TGG - chr5_3:42243805-42243824 Msa0812290:five_prime_UTR 15.0%
!! AAATGAAAGAATTAGAATTA+GGG + chr5_3:42242117-42242136 None:intergenic 15.0%
!!! TTTAATTTTCAGTTATGATT+TGG - chr5_3:42242448-42242467 Msa0812290:three_prime_UTR 15.0%
!!! TTAATTTTCAGTTATGATTT+GGG - chr5_3:42242449-42242468 Msa0812290:three_prime_UTR 15.0%
!! TATATTAACAGTTTAAACAA+TGG - chr5_3:42243115-42243134 Msa0812290:intron 15.0%
!! AATACTAATTATAGAGAAAT+AGG - chr5_3:42243255-42243274 Msa0812290:intron 15.0%
!!! GAATTTTTTTTTTAATCAAG+TGG + chr5_3:42243496-42243515 None:intergenic 15.0%
!! TTAAAAAAAAAATTCAAGCA+AGG - chr5_3:42243501-42243520 Msa0812290:intron 15.0%
!! AGAATAAAGAGTTAATTTAA+TGG + chr5_3:42243793-42243812 None:intergenic 15.0%
!!! TTTATTCTCTTAAATTTTGT+TGG - chr5_3:42243805-42243824 Msa0812290:five_prime_UTR 15.0%
!! GAAATGAAAGAATTAGAATT+AGG + chr5_3:42242118-42242137 None:intergenic 20.0%
!! TATCTTATAGGACTTAATTA+GGG + chr5_3:42242745-42242764 None:intergenic 20.0%
!! TTATCTTATAGGACTTAATT+AGG + chr5_3:42242746-42242765 None:intergenic 20.0%
!! CTGTTAATATACATTATCAT+AGG + chr5_3:42243106-42243125 None:intergenic 20.0%
!!! ATATTGACTTAACTCTTTTT+TGG - chr5_3:42243148-42243167 Msa0812290:intron 20.0%
!!! TATTGACTTAACTCTTTTTT+GGG - chr5_3:42243149-42243168 Msa0812290:intron 20.0%
!!! ATTGTGTAAATGATTTTTCA+CGG + chr5_3:42243455-42243474 None:intergenic 20.0%
!!! TATCATAACATTTTAAATCG+TGG - chr5_3:42243630-42243649 Msa0812290:intron 20.0%
!!! TTAATGGTATGAACTTTTTA+AGG + chr5_3:42243777-42243796 None:intergenic 20.0%
!!! TAAATTTTGTTGGATATACA+AGG - chr5_3:42243815-42243834 Msa0812290:five_prime_UTR 20.0%
!! GAAATGAAAGAATTAGAATT+AGG + chr5_3:42242118-42242137 None:intergenic 20.0%
!! TATCTTATAGGACTTAATTA+GGG + chr5_3:42242745-42242764 None:intergenic 20.0%
!! TTATCTTATAGGACTTAATT+AGG + chr5_3:42242746-42242765 None:intergenic 20.0%
!! CTGTTAATATACATTATCAT+AGG + chr5_3:42243106-42243125 None:intergenic 20.0%
!!! ATATTGACTTAACTCTTTTT+TGG - chr5_3:42243148-42243167 Msa0812290:intron 20.0%
!!! TATTGACTTAACTCTTTTTT+GGG - chr5_3:42243149-42243168 Msa0812290:intron 20.0%
!!! ATTGTGTAAATGATTTTTCA+CGG + chr5_3:42243455-42243474 None:intergenic 20.0%
!!! TATCATAACATTTTAAATCG+TGG - chr5_3:42243630-42243649 Msa0812290:intron 20.0%
!!! TTAATGGTATGAACTTTTTA+AGG + chr5_3:42243777-42243796 None:intergenic 20.0%
!!! TAAATTTTGTTGGATATACA+AGG - chr5_3:42243815-42243834 Msa0812290:five_prime_UTR 20.0%
! CGATTTCGAAATATACAATT+GGG + chr5_3:42242071-42242090 None:intergenic 25.0%
! TCGATTTCGAAATATACAAT+TGG + chr5_3:42242072-42242091 None:intergenic 25.0%
! AAGAATTAGAATTAGGGTTA+GGG + chr5_3:42242111-42242130 None:intergenic 25.0%
! AAAGAATTAGAATTAGGGTT+AGG + chr5_3:42242112-42242131 None:intergenic 25.0%
!! CAGAAATCAAAACTTTTCAA+GGG - chr5_3:42242162-42242181 Msa0812290:three_prime_UTR 25.0%
!! AATTGTGAACTTTTCTTTAC+AGG - chr5_3:42242309-42242328 Msa0812290:three_prime_UTR 25.0%
!! ATTGTGAACTTTTCTTTACA+GGG - chr5_3:42242310-42242329 Msa0812290:three_prime_UTR 25.0%
! GAAAATTAGAGAAAAGCAAA+AGG + chr5_3:42242394-42242413 None:intergenic 25.0%
!!! AATTTGTAGTGTGTTTTTTG+TGG - chr5_3:42242420-42242439 Msa0812290:three_prime_UTR 25.0%
! TTGATAAGCACTTATCTTAT+AGG + chr5_3:42242757-42242776 None:intergenic 25.0%
!!! CATACTTTCTTGTTTTACTT+TGG + chr5_3:42242803-42242822 None:intergenic 25.0%
! CAAAGTAAAACAAGAAAGTA+TGG - chr5_3:42242801-42242820 Msa0812290:intron 25.0%
!! AAGCCATAAATTGTTTTCAT+CGG - chr5_3:42242841-42242860 Msa0812290:intron 25.0%
!!! ATTGACTTAACTCTTTTTTG+GGG - chr5_3:42243150-42243169 Msa0812290:intron 25.0%
! CGATTTCGAAATATACAATT+GGG + chr5_3:42242071-42242090 None:intergenic 25.0%
! TCGATTTCGAAATATACAAT+TGG + chr5_3:42242072-42242091 None:intergenic 25.0%
! AAGAATTAGAATTAGGGTTA+GGG + chr5_3:42242111-42242130 None:intergenic 25.0%
! AAAGAATTAGAATTAGGGTT+AGG + chr5_3:42242112-42242131 None:intergenic 25.0%
!! CAGAAATCAAAACTTTTCAA+GGG - chr5_3:42242162-42242181 Msa0812290:three_prime_UTR 25.0%
!! AATTGTGAACTTTTCTTTAC+AGG - chr5_3:42242309-42242328 Msa0812290:three_prime_UTR 25.0%
!! ATTGTGAACTTTTCTTTACA+GGG - chr5_3:42242310-42242329 Msa0812290:three_prime_UTR 25.0%
! GAAAATTAGAGAAAAGCAAA+AGG + chr5_3:42242394-42242413 None:intergenic 25.0%
!!! AATTTGTAGTGTGTTTTTTG+TGG - chr5_3:42242420-42242439 Msa0812290:three_prime_UTR 25.0%
! TTGATAAGCACTTATCTTAT+AGG + chr5_3:42242757-42242776 None:intergenic 25.0%
!!! CATACTTTCTTGTTTTACTT+TGG + chr5_3:42242803-42242822 None:intergenic 25.0%
! CAAAGTAAAACAAGAAAGTA+TGG - chr5_3:42242801-42242820 Msa0812290:intron 25.0%
!! AAGCCATAAATTGTTTTCAT+CGG - chr5_3:42242841-42242860 Msa0812290:intron 25.0%
!!! ATTGACTTAACTCTTTTTTG+GGG - chr5_3:42243150-42243169 Msa0812290:intron 25.0%
! GCAGAAATCAAAACTTTTCA+AGG - chr5_3:42242161-42242180 Msa0812290:three_prime_UTR 30.0%
! GAATCGATTTCTTCTTAGTT+TGG + chr5_3:42242188-42242207 None:intergenic 30.0%
ATATCCTTTGTAACTTTCGT+TGG + chr5_3:42242351-42242370 None:intergenic 30.0%
AACGAAAGTTACAAAGGATA+TGG - chr5_3:42242350-42242369 Msa0812290:three_prime_UTR 30.0%
!! TTCAGTTATGATTTGGGTTT+TGG - chr5_3:42242455-42242474 Msa0812290:three_prime_UTR 30.0%
CAAAATAAGCTCAATCCAAA+CGG + chr5_3:42242538-42242557 None:intergenic 30.0%
! TAATTAGGGTCTTTTTGGAT+TGG + chr5_3:42242731-42242750 None:intergenic 30.0%
TTATGGCAGTAAATAAGCTT+AGG - chr5_3:42242961-42242980 Msa0812290:intron 30.0%
AACAGTTTAAACAATGGTTG+AGG - chr5_3:42243121-42243140 Msa0812290:intron 30.0%
GTTCTATGAATCTCAGAATT+TGG + chr5_3:42243300-42243319 None:intergenic 30.0%
AAAGCTAAGAAATGTGTGTA+TGG - chr5_3:42243588-42243607 Msa0812290:CDS 30.0%
! TGGTCATGATATTTGATGAT+TGG - chr5_3:42243650-42243669 Msa0812290:intron 30.0%
! GCAGAAATCAAAACTTTTCA+AGG - chr5_3:42242161-42242180 Msa0812290:three_prime_UTR 30.0%
! GAATCGATTTCTTCTTAGTT+TGG + chr5_3:42242188-42242207 None:intergenic 30.0%
ATATCCTTTGTAACTTTCGT+TGG + chr5_3:42242351-42242370 None:intergenic 30.0%
AACGAAAGTTACAAAGGATA+TGG - chr5_3:42242350-42242369 Msa0812290:three_prime_UTR 30.0%
!! TTCAGTTATGATTTGGGTTT+TGG - chr5_3:42242455-42242474 Msa0812290:three_prime_UTR 30.0%
CAAAATAAGCTCAATCCAAA+CGG + chr5_3:42242538-42242557 None:intergenic 30.0%
! TAATTAGGGTCTTTTTGGAT+TGG + chr5_3:42242731-42242750 None:intergenic 30.0%
TTATGGCAGTAAATAAGCTT+AGG - chr5_3:42242961-42242980 Msa0812290:intron 30.0%
AACAGTTTAAACAATGGTTG+AGG - chr5_3:42243121-42243140 Msa0812290:intron 30.0%
GTTCTATGAATCTCAGAATT+TGG + chr5_3:42243300-42243319 None:intergenic 30.0%
AAAGCTAAGAAATGTGTGTA+TGG - chr5_3:42243588-42243607 Msa0812290:CDS 30.0%
! TGGTCATGATATTTGATGAT+TGG - chr5_3:42243650-42243669 Msa0812290:intron 30.0%
AATTTACCTTTGCGAGTTTG+AGG + chr5_3:42242234-42242253 None:intergenic 35.0%
! GAAGTATTCATGTTTCTGCT+TGG - chr5_3:42242495-42242514 Msa0812290:three_prime_UTR 35.0%
CTGCTTGGATAAACAGATTA+AGG - chr5_3:42242510-42242529 Msa0812290:three_prime_UTR 35.0%
! GGACTTAATTAGGGTCTTTT+TGG + chr5_3:42242736-42242755 None:intergenic 35.0%
CAGCCGATGAAAACAATTTA+TGG + chr5_3:42242847-42242866 None:intergenic 35.0%
AAGACACTAGCAAGAGTTTA+TGG - chr5_3:42242944-42242963 Msa0812290:intron 35.0%
AATACACAAGCTTCTGCATT+TGG + chr5_3:42243007-42243026 None:intergenic 35.0%
!!! CTTTTTTGGGGCTTATCTTA+TGG - chr5_3:42243162-42243181 Msa0812290:intron 35.0%
! GTAACTTGTAAGCACTTGTA+CGG - chr5_3:42243189-42243208 Msa0812290:intron 35.0%
AGATTCATAGAACGAAGCTA+AGG - chr5_3:42243307-42243326 Msa0812290:intron 35.0%
GATTCATAGAACGAAGCTAA+GGG - chr5_3:42243308-42243327 Msa0812290:intron 35.0%
AATCAAGTGGTTGCCAAAAA+GGG + chr5_3:42243483-42243502 None:intergenic 35.0%
TAATCAAGTGGTTGCCAAAA+AGG + chr5_3:42243484-42243503 None:intergenic 35.0%
! AATGTGTGTATGGACTGTTA+CGG - chr5_3:42243598-42243617 Msa0812290:CDS 35.0%
!! GCATTGATGAAAAGCGTTAT+TGG - chr5_3:42243853-42243872 Msa0812290:five_prime_UTR 35.0%
AATTTACCTTTGCGAGTTTG+AGG + chr5_3:42242234-42242253 None:intergenic 35.0%
! GAAGTATTCATGTTTCTGCT+TGG - chr5_3:42242495-42242514 Msa0812290:three_prime_UTR 35.0%
CTGCTTGGATAAACAGATTA+AGG - chr5_3:42242510-42242529 Msa0812290:three_prime_UTR 35.0%
! GGACTTAATTAGGGTCTTTT+TGG + chr5_3:42242736-42242755 None:intergenic 35.0%
CAGCCGATGAAAACAATTTA+TGG + chr5_3:42242847-42242866 None:intergenic 35.0%
AAGACACTAGCAAGAGTTTA+TGG - chr5_3:42242944-42242963 Msa0812290:intron 35.0%
AATACACAAGCTTCTGCATT+TGG + chr5_3:42243007-42243026 None:intergenic 35.0%
!!! CTTTTTTGGGGCTTATCTTA+TGG - chr5_3:42243162-42243181 Msa0812290:intron 35.0%
! GTAACTTGTAAGCACTTGTA+CGG - chr5_3:42243189-42243208 Msa0812290:intron 35.0%
AGATTCATAGAACGAAGCTA+AGG - chr5_3:42243307-42243326 Msa0812290:intron 35.0%
GATTCATAGAACGAAGCTAA+GGG - chr5_3:42243308-42243327 Msa0812290:intron 35.0%
AATCAAGTGGTTGCCAAAAA+GGG + chr5_3:42243483-42243502 None:intergenic 35.0%
TAATCAAGTGGTTGCCAAAA+AGG + chr5_3:42243484-42243503 None:intergenic 35.0%
! AATGTGTGTATGGACTGTTA+CGG - chr5_3:42243598-42243617 Msa0812290:CDS 35.0%
!! GCATTGATGAAAAGCGTTAT+TGG - chr5_3:42243853-42243872 Msa0812290:five_prime_UTR 35.0%
! AAAGTACCTCAAACTCGCAA+AGG - chr5_3:42242225-42242244 Msa0812290:three_prime_UTR 40.0%
GAAGCCAACGAAAGTTACAA+AGG - chr5_3:42242344-42242363 Msa0812290:three_prime_UTR 40.0%
AAACAGATTAAGGCTCCGTT+TGG - chr5_3:42242520-42242539 Msa0812290:three_prime_UTR 40.0%
ACAAGCTTCTGCATTTGGAT+CGG + chr5_3:42243002-42243021 None:intergenic 40.0%
GGAAGCCATTAACACTACAA+CGG - chr5_3:42243213-42243232 Msa0812290:intron 40.0%
GAAGCCATTAACACTACAAC+GGG - chr5_3:42243214-42243233 Msa0812290:intron 40.0%
!! GAAGCTAAGGGTTGATTTTG+AGG - chr5_3:42243320-42243339 Msa0812290:intron 40.0%
ATATCTGCAGAAGAAGACTG+AGG - chr5_3:42243396-42243415 Msa0812290:intron 40.0%
TATCTGCAGAAGAAGACTGA+GGG - chr5_3:42243397-42243416 Msa0812290:intron 40.0%
AGGATCAGTTGAACATCTGT+CGG + chr5_3:42243427-42243446 None:intergenic 40.0%
AGATGTTCAACTGATCCTCT+CGG - chr5_3:42243429-42243448 Msa0812290:intron 40.0%
! TACACAATACGCACCCTTTT+TGG - chr5_3:42243467-42243486 Msa0812290:intron 40.0%
AAGCAAGGAGAATTGTCACA+TGG - chr5_3:42243516-42243535 Msa0812290:intron 40.0%
AGCAAGGAGAATTGTCACAT+GGG - chr5_3:42243517-42243536 Msa0812290:intron 40.0%
ATTGTCACATGGGAGTAGAT+AGG - chr5_3:42243527-42243546 Msa0812290:intron 40.0%
! ATGTGTGTATGGACTGTTAC+GGG - chr5_3:42243599-42243618 Msa0812290:CDS 40.0%
! AAAGTACCTCAAACTCGCAA+AGG - chr5_3:42242225-42242244 Msa0812290:three_prime_UTR 40.0%
GAAGCCAACGAAAGTTACAA+AGG - chr5_3:42242344-42242363 Msa0812290:three_prime_UTR 40.0%
AAACAGATTAAGGCTCCGTT+TGG - chr5_3:42242520-42242539 Msa0812290:three_prime_UTR 40.0%
ACAAGCTTCTGCATTTGGAT+CGG + chr5_3:42243002-42243021 None:intergenic 40.0%
GGAAGCCATTAACACTACAA+CGG - chr5_3:42243213-42243232 Msa0812290:intron 40.0%
GAAGCCATTAACACTACAAC+GGG - chr5_3:42243214-42243233 Msa0812290:intron 40.0%
!! GAAGCTAAGGGTTGATTTTG+AGG - chr5_3:42243320-42243339 Msa0812290:intron 40.0%
ATATCTGCAGAAGAAGACTG+AGG - chr5_3:42243396-42243415 Msa0812290:intron 40.0%
TATCTGCAGAAGAAGACTGA+GGG - chr5_3:42243397-42243416 Msa0812290:intron 40.0%
AGGATCAGTTGAACATCTGT+CGG + chr5_3:42243427-42243446 None:intergenic 40.0%
AGATGTTCAACTGATCCTCT+CGG - chr5_3:42243429-42243448 Msa0812290:intron 40.0%
! TACACAATACGCACCCTTTT+TGG - chr5_3:42243467-42243486 Msa0812290:intron 40.0%
AAGCAAGGAGAATTGTCACA+TGG - chr5_3:42243516-42243535 Msa0812290:intron 40.0%
AGCAAGGAGAATTGTCACAT+GGG - chr5_3:42243517-42243536 Msa0812290:intron 40.0%
ATTGTCACATGGGAGTAGAT+AGG - chr5_3:42243527-42243546 Msa0812290:intron 40.0%
! ATGTGTGTATGGACTGTTAC+GGG - chr5_3:42243599-42243618 Msa0812290:CDS 40.0%
TCGATTCCTGCTAATCGTCA+TGG - chr5_3:42242201-42242220 Msa0812290:three_prime_UTR 45.0%
ACTTTGCCATGACGATTAGC+AGG + chr5_3:42242210-42242229 None:intergenic 45.0%
ACTTGTAAGCACTTGTACGG+TGG - chr5_3:42243192-42243211 Msa0812290:intron 45.0%
TCCTCCCGTTGTAGTGTTAA+TGG + chr5_3:42243221-42243240 None:intergenic 45.0%
TTAAACCAACCCTCTATGCC+TGG + chr5_3:42243349-42243368 None:intergenic 45.0%
!! GGCATAGAGGGTTGGTTTAA+TGG - chr5_3:42243349-42243368 Msa0812290:intron 45.0%
!! GAGGGTTGGTTTAATGGCAT+TGG - chr5_3:42243355-42243374 Msa0812290:intron 45.0%
GGATCAGTTGAACATCTGTC+GGG + chr5_3:42243426-42243445 None:intergenic 45.0%
! AATGATTTTTCACGGCCGAG+AGG + chr5_3:42243447-42243466 None:intergenic 45.0%
TCGATTCCTGCTAATCGTCA+TGG - chr5_3:42242201-42242220 Msa0812290:three_prime_UTR 45.0%
ACTTTGCCATGACGATTAGC+AGG + chr5_3:42242210-42242229 None:intergenic 45.0%
ACTTGTAAGCACTTGTACGG+TGG - chr5_3:42243192-42243211 Msa0812290:intron 45.0%
TCCTCCCGTTGTAGTGTTAA+TGG + chr5_3:42243221-42243240 None:intergenic 45.0%
TTAAACCAACCCTCTATGCC+TGG + chr5_3:42243349-42243368 None:intergenic 45.0%
!! GGCATAGAGGGTTGGTTTAA+TGG - chr5_3:42243349-42243368 Msa0812290:intron 45.0%
!! GAGGGTTGGTTTAATGGCAT+TGG - chr5_3:42243355-42243374 Msa0812290:intron 45.0%
GGATCAGTTGAACATCTGTC+GGG + chr5_3:42243426-42243445 None:intergenic 45.0%
! AATGATTTTTCACGGCCGAG+AGG + chr5_3:42243447-42243466 None:intergenic 45.0%
GCCATTAACACTACAACGGG+AGG - chr5_3:42243217-42243236 Msa0812290:intron 50.0%
!! GGGTTGATTTTGAGGACACC+AGG - chr5_3:42243328-42243347 Msa0812290:intron 50.0%
TTTGAGGACACCAGGCATAG+AGG - chr5_3:42243336-42243355 Msa0812290:intron 50.0%
TTGAGGACACCAGGCATAGA+GGG - chr5_3:42243337-42243356 Msa0812290:intron 50.0%
GCCATTAACACTACAACGGG+AGG - chr5_3:42243217-42243236 Msa0812290:intron 50.0%
!! GGGTTGATTTTGAGGACACC+AGG - chr5_3:42243328-42243347 Msa0812290:intron 50.0%
TTTGAGGACACCAGGCATAG+AGG - chr5_3:42243336-42243355 Msa0812290:intron 50.0%
TTGAGGACACCAGGCATAGA+GGG - chr5_3:42243337-42243356 Msa0812290:intron 50.0%
GGACACCAGGCATAGAGGGT+TGG - chr5_3:42243341-42243360 Msa0812290:intron 60.0%
GGACACCAGGCATAGAGGGT+TGG - chr5_3:42243341-42243360 Msa0812290:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 42242053 42243899 42242053 ID=Msa0812290;Name=Msa0812290
chr5_3 mRNA 42242053 42243899 42242053 ID=Msa0812290-mRNA-1;Parent=Msa0812290;Name=Msa0812290-mRNA-1;_AED=0.19;_eAED=0.19;_QI=103|1|1|1|0.5|0.66|3|707|48
chr5_3 exon 42242053 42242762 42242053 ID=Msa0812290-mRNA-1:exon:12988;Parent=Msa0812290-mRNA-1
chr5_3 exon 42243569 42243621 42243569 ID=Msa0812290-mRNA-1:exon:12987;Parent=Msa0812290-mRNA-1
chr5_3 exon 42243706 42243899 42243706 ID=Msa0812290-mRNA-1:exon:12986;Parent=Msa0812290-mRNA-1
chr5_3 five_prime_UTR 42243797 42243899 42243797 ID=Msa0812290-mRNA-1:five_prime_utr;Parent=Msa0812290-mRNA-1
chr5_3 CDS 42243706 42243796 42243706 ID=Msa0812290-mRNA-1:cds;Parent=Msa0812290-mRNA-1
chr5_3 CDS 42243569 42243621 42243569 ID=Msa0812290-mRNA-1:cds;Parent=Msa0812290-mRNA-1
chr5_3 CDS 42242760 42242762 42242760 ID=Msa0812290-mRNA-1:cds;Parent=Msa0812290-mRNA-1
chr5_3 three_prime_UTR 42242053 42242759 42242053 ID=Msa0812290-mRNA-1:three_prime_utr;Parent=Msa0812290-mRNA-1
Gene Sequence

>Msa0812290

ATGACGCAGAAATCAAAACTTTTCAAGGGCCAAACTAAGAAGAAATCGATTCCTGCTAATCGTCATGGCAAAGTACCTCAAACTCGCAAAGGGAAGAGATTTGTGAAGCCAACGAAAGTTACAAAGGATATGGATGCTGATCGTTAA
Protein sequence

>Msa0812290

MTQKSKLFKGQTKKKSIPANRHGKVPQTRKGKRFVKPTKVTKDMDADR*