AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0817120


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817120 MtrunA17_Chr5g0404751 91.026 78 7 0 1 78 1 78 4.46e-48 149
Msa0817120 MtrunA17_Chr2g0299021 48.276 58 30 0 21 78 71 128 8.46e-14 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817120 AT1G77370.1 56.338 71 31 0 8 78 9 79 5.28e-22 83.6
Msa0817120 AT1G77370.2 64.912 57 20 0 24 80 25 81 9.08e-22 84.0
Msa0817120 AT5G20500.1 40.278 72 43 0 7 78 6 77 1.90e-13 62.0

Find 30 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCCATTCTCAAACAAAA+AGG 0.217348 5_3:-51962437 None:intergenic
ATTTCGTTGTCATTGTTAAT+TGG 0.231844 5_3:+51964449 Msa0817120:three_prime_UTR
AAATCAGTGTCGTGTCAAAT+TGG 0.237203 5_3:+51962308 Msa0817120:five_prime_UTR
ATTTGACATTGGTTCTTTGT+AGG 0.328186 5_3:-51964646 None:intergenic
GACACGCGTGGAAAACAATA+AGG 0.342799 5_3:+51962268 None:intergenic
TACGTACGGGCAATAGGATT+TGG 0.352701 5_3:-51962527 None:intergenic
TATATATACGTACGGGCAAT+AGG 0.386425 5_3:-51962533 None:intergenic
GGAAACTGAATTTGAAGCTT+CGG 0.429054 5_3:-51962458 None:intergenic
AATCAGTGTCGTGTCAAATT+GGG 0.440854 5_3:+51962309 Msa0817120:five_prime_UTR
AGCAGTGGAGCTCTAATTGT+TGG 0.456983 5_3:+51964421 Msa0817120:three_prime_UTR
AACAGAACTATTCTATTCAA+TGG 0.462801 5_3:-51964691 None:intergenic
ATCTATAATTTGTATGAATG+TGG 0.490361 5_3:+51964224 Msa0817120:three_prime_UTR
TCTATAATTTGTATGAATGT+GGG 0.491540 5_3:+51964225 Msa0817120:three_prime_UTR
ATTGAATCTATCTCAAGTAA+TGG 0.497398 5_3:+51964594 Msa0817120:three_prime_UTR
CGTGGAAAACAATAAGGGTC+TGG 0.507148 5_3:+51962274 None:intergenic
GGAGAAGATGGTGATTCTGT+TGG 0.515760 5_3:-51962506 None:intergenic
GCAATAGGATTTGGAGAAGA+TGG 0.516584 5_3:-51962518 None:intergenic
AATCAAAGAAGTAAAACTAC+AGG 0.537670 5_3:+51964623 Msa0817120:three_prime_UTR
ACACGCGTGGAAAACAATAA+GGG 0.548482 5_3:+51962269 None:intergenic
GTTTCTGCAATTCACCACTC+TGG 0.562376 5_3:-51964155 None:intergenic
AAGACAAGACAATTTGACAT+TGG 0.563313 5_3:-51964657 None:intergenic
GATTCAATGAAACAATACTC+TGG 0.570833 5_3:-51964579 None:intergenic
CTTCGATATACATGAAAATG+CGG 0.575457 5_3:-51964252 None:intergenic
CTATAATTTGTATGAATGTG+GGG 0.594049 5_3:+51964226 Msa0817120:three_prime_UTR
TTCGATATACATGAAAATGC+GGG 0.595061 5_3:-51964251 None:intergenic
CGAAGGTTTCAAGCTTCTAG+CGG 0.615435 5_3:+51964270 Msa0817120:three_prime_UTR
TGATTGTATGCTTGAGACTG+AGG 0.642233 5_3:+51964192 Msa0817120:CDS
GAAAGTACGATCATGAGCAG+TGG 0.651793 5_3:+51964406 Msa0817120:three_prime_UTR
GAAGAATGAAGATGTTACAG+TGG 0.663384 5_3:+51962377 Msa0817120:exon
CTCAGTGCTGCAGTCCAGAG+TGG 0.673667 5_3:+51964141 Msa0817120:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTATTGTATTTATTTTTTA+TGG - chr5_3:51964743-51964762 None:intergenic 10.0%
!! AAAAAAAAAAACTGCTTTAA+TGG + chr5_3:51963065-51963084 Msa0817120:intron 15.0%
!!! AAGGGAATATTTATTATTTT+CGG - chr5_3:51963881-51963900 None:intergenic 15.0%
!!! TATTTAGCTTTTAAAGTATA+TGG - chr5_3:51963982-51964001 None:intergenic 15.0%
!! TATCTATATGTCATTATAAA+AGG + chr5_3:51964054-51964073 Msa0817120:intron 15.0%
!!! AATACTTTTTCATGTTTTTT+AGG + chr5_3:51964296-51964315 Msa0817120:three_prime_UTR 15.0%
!!! ATTAAGTTGTTTATCAAACA+AGG - chr5_3:51962754-51962773 None:intergenic 20.0%
!!! TTAAGTTGTTTATACAAACA+AGG - chr5_3:51963467-51963486 None:intergenic 20.0%
!! TCATATAGTTTAAGAACTAA+AGG - chr5_3:51963741-51963760 None:intergenic 20.0%
!! TAAACTATATGAAAATAACG+AGG + chr5_3:51963749-51963768 Msa0817120:intron 20.0%
!!! CTTATATTTTTGGATATAGA+GGG - chr5_3:51963803-51963822 None:intergenic 20.0%
!! CTCTATATCCAAAAATATAA+GGG + chr5_3:51963802-51963821 Msa0817120:intron 20.0%
!!! TGATAGAATTTTTTTATGAG+AGG + chr5_3:51963847-51963866 Msa0817120:intron 20.0%
!!! ATACTTTAAAAGCTAAATAG+AGG + chr5_3:51963983-51964002 Msa0817120:intron 20.0%
!! TATGTCATTATAAAAGGTTA+AGG + chr5_3:51964060-51964079 Msa0817120:intron 20.0%
!!! ATCTATAATTTGTATGAATG+TGG + chr5_3:51964224-51964243 Msa0817120:three_prime_UTR 20.0%
!!! TCTATAATTTGTATGAATGT+GGG + chr5_3:51964225-51964244 Msa0817120:three_prime_UTR 20.0%
!!! ATGTTTTTTAGGTATTTACT+AGG + chr5_3:51964307-51964326 Msa0817120:three_prime_UTR 20.0%
!!! ATGTTTTCATTTTGTTCTAT+AGG + chr5_3:51964384-51964403 Msa0817120:three_prime_UTR 20.0%
!!! AATTGTAGTTTTCAATAATC+TGG + chr5_3:51964477-51964496 Msa0817120:three_prime_UTR 20.0%
! AAGAGAGTTATATATACGTA+CGG - chr5_3:51962544-51962563 None:intergenic 25.0%
! TTCAATTATTATCACGTGTT+AGG + chr5_3:51962569-51962588 Msa0817120:intron 25.0%
! TCAATTATTATCACGTGTTA+GGG + chr5_3:51962570-51962589 Msa0817120:intron 25.0%
! GTTTAATTAATATGCTCTGT+CGG + chr5_3:51962807-51962826 Msa0817120:intron 25.0%
! GTTGTGATATTCATCAATTA+AGG - chr5_3:51962868-51962887 None:intergenic 25.0%
! ATTCAAATAGTATACTCAGT+CGG - chr5_3:51962946-51962965 None:intergenic 25.0%
! GAAATAACCTATACATAAGA+GGG - chr5_3:51962982-51963001 None:intergenic 25.0%
! AGAAATAACCTATACATAAG+AGG - chr5_3:51962983-51963002 None:intergenic 25.0%
!! TTTTCATAAGTGTTTATGTC+AGG + chr5_3:51963150-51963169 Msa0817120:intron 25.0%
! AATGATAACAGTTGTCTATT+TGG - chr5_3:51963198-51963217 None:intergenic 25.0%
! TATGAAAATAACGAGGAATT+TGG + chr5_3:51963756-51963775 Msa0817120:intron 25.0%
!!! CCTTATATTTTTGGATATAG+AGG - chr5_3:51963804-51963823 None:intergenic 25.0%
! CCTCTATATCCAAAAATATA+AGG + chr5_3:51963801-51963820 Msa0817120:intron 25.0%
!! ACAAATTTTCACATACTCAA+GGG - chr5_3:51963899-51963918 None:intergenic 25.0%
! TAATGAAGGGATTAAATTGA+AGG + chr5_3:51964015-51964034 Msa0817120:intron 25.0%
!! CTATAATTTGTATGAATGTG+GGG + chr5_3:51964226-51964245 Msa0817120:three_prime_UTR 25.0%
! ATTTCGTTGTCATTGTTAAT+TGG + chr5_3:51964449-51964468 Msa0817120:three_prime_UTR 25.0%
! ATTGAATCTATCTCAAGTAA+TGG + chr5_3:51964594-51964613 Msa0817120:three_prime_UTR 25.0%
! AATCAAAGAAGTAAAACTAC+AGG + chr5_3:51964623-51964642 Msa0817120:three_prime_UTR 25.0%
! AACAGAACTATTCTATTCAA+TGG - chr5_3:51964694-51964713 None:intergenic 25.0%
!!! TGTTTTTGTACCAAACAAAA+TGG + chr5_3:51964710-51964729 Msa0817120:three_prime_UTR 25.0%
CTCAAACAAAAAGGGTAAAA+CGG - chr5_3:51962431-51962450 None:intergenic 30.0%
!! TTACCCTTTTTGTTTGAGAA+TGG + chr5_3:51962433-51962452 Msa0817120:CDS 30.0%
GTTCCATTCTCAAACAAAAA+GGG - chr5_3:51962439-51962458 None:intergenic 30.0%
AGAGAGTTATATATACGTAC+GGG - chr5_3:51962543-51962562 None:intergenic 30.0%
! AATGTTTCTCTGATTTTCGT+AGG + chr5_3:51962611-51962630 Msa0817120:intron 30.0%
AAATCAAGCTCAACAACAAA+AGG - chr5_3:51962682-51962701 None:intergenic 30.0%
TAACAGTTGTCTATTTGGAT+TGG - chr5_3:51963193-51963212 None:intergenic 30.0%
! TTTTGTGCTTGTTTAGATGA+TGG + chr5_3:51963249-51963268 Msa0817120:intron 30.0%
TTTGTGCTTGTTTAGATGAT+GGG + chr5_3:51963250-51963269 Msa0817120:intron 30.0%
!! TTCTTAGACTTTTGCTTTTC+TGG + chr5_3:51963376-51963395 Msa0817120:intron 30.0%
!! TCTTAGACTTTTGCTTTTCT+GGG + chr5_3:51963377-51963396 Msa0817120:intron 30.0%
! TTCTCTCACCCTTATATTTT+TGG - chr5_3:51963813-51963832 None:intergenic 30.0%
! GACAAATTTTCACATACTCA+AGG - chr5_3:51963900-51963919 None:intergenic 30.0%
CATTAGTACTTAAGATGCTA+GGG - chr5_3:51963931-51963950 None:intergenic 30.0%
TCATTAGTACTTAAGATGCT+AGG - chr5_3:51963932-51963951 None:intergenic 30.0%
TCTTAAGTACTAATGAGACA+AGG + chr5_3:51963936-51963955 Msa0817120:intron 30.0%
CTTAAGTACTAATGAGACAA+GGG + chr5_3:51963937-51963956 Msa0817120:intron 30.0%
TTAAGTACTAATGAGACAAG+GGG + chr5_3:51963938-51963957 Msa0817120:intron 30.0%
! AGAGGAGATAATGATAATGA+AGG + chr5_3:51964001-51964020 Msa0817120:intron 30.0%
! GAGGAGATAATGATAATGAA+GGG + chr5_3:51964002-51964021 Msa0817120:intron 30.0%
ATGAGCAAACTTTGTAAGAA+TGG - chr5_3:51964107-51964126 None:intergenic 30.0%
TTCGATATACATGAAAATGC+GGG - chr5_3:51964254-51964273 None:intergenic 30.0%
CTTCGATATACATGAAAATG+CGG - chr5_3:51964255-51964274 None:intergenic 30.0%
GATTCAATGAAACAATACTC+TGG - chr5_3:51964582-51964601 None:intergenic 30.0%
!! ATTTGACATTGGTTCTTTGT+AGG - chr5_3:51964649-51964668 None:intergenic 30.0%
AAGACAAGACAATTTGACAT+TGG - chr5_3:51964660-51964679 None:intergenic 30.0%
AAATCAGTGTCGTGTCAAAT+TGG + chr5_3:51962308-51962327 Msa0817120:five_prime_UTR 35.0%
AATCAGTGTCGTGTCAAATT+GGG + chr5_3:51962309-51962328 Msa0817120:five_prime_UTR 35.0%
GAAGAATGAAGATGTTACAG+TGG + chr5_3:51962377-51962396 Msa0817120:exon 35.0%
GGTTCCATTCTCAAACAAAA+AGG - chr5_3:51962440-51962459 None:intergenic 35.0%
! GGAAACTGAATTTGAAGCTT+CGG - chr5_3:51962461-51962480 None:intergenic 35.0%
!! GATTGCGTTTTGAACAAAAG+CGG - chr5_3:51962482-51962501 None:intergenic 35.0%
TATATATACGTACGGGCAAT+AGG - chr5_3:51962536-51962555 None:intergenic 35.0%
TAAGGAGACAACACAATCAT+CGG - chr5_3:51962850-51962869 None:intergenic 35.0%
! ATTGTTTTCGCGTAAGCTAT+AGG + chr5_3:51963017-51963036 Msa0817120:intron 35.0%
!! AGATGATGGGTATCAAATTC+AGG + chr5_3:51963263-51963282 Msa0817120:intron 35.0%
!! GATGATGGGTATCAAATTCA+GGG + chr5_3:51963264-51963283 Msa0817120:intron 35.0%
! TTTGTCTATGGGAAGCATAT+TGG + chr5_3:51963330-51963349 Msa0817120:intron 35.0%
ATATAAGGGTGAGAGAAGAT+AGG + chr5_3:51963816-51963835 Msa0817120:intron 35.0%
TATAAGGGTGAGAGAAGATA+GGG + chr5_3:51963817-51963836 Msa0817120:intron 35.0%
TTAAGATGCTAGGGATTAAC+AGG - chr5_3:51963922-51963941 None:intergenic 35.0%
TGGATAGAGAAACACAAAGA+GGG - chr5_3:51964087-51964106 None:intergenic 35.0%
ATGGATAGAGAAACACAAAG+AGG - chr5_3:51964088-51964107 None:intergenic 35.0%
! CGCATTTTCATGTATATCGA+AGG + chr5_3:51964253-51964272 Msa0817120:three_prime_UTR 35.0%
!!! ACATGTACTTTTAGCTTGTC+TGG + chr5_3:51964336-51964355 Msa0817120:three_prime_UTR 35.0%
!! TGGTACAAAGCCATTTTGTT+TGG - chr5_3:51964723-51964742 None:intergenic 35.0%
GCAATAGGATTTGGAGAAGA+TGG - chr5_3:51962521-51962540 None:intergenic 40.0%
!! CCTATACATAAGAGGGTATC+AGG - chr5_3:51962975-51962994 None:intergenic 40.0%
CCTGATACCCTCTTATGTAT+AGG + chr5_3:51962972-51962991 Msa0817120:intron 40.0%
ACTGCTTTAATGGTCTGTCT+TGG + chr5_3:51963075-51963094 Msa0817120:intron 40.0%
! TCAAATTCAGGGTGTTCTTC+TGG + chr5_3:51963275-51963294 Msa0817120:intron 40.0%
GGTGTTCTTCTGGATTTGAT+TGG + chr5_3:51963285-51963304 Msa0817120:intron 40.0%
TCCCACAAGTGTTTGTCTAT+GGG + chr5_3:51963319-51963338 Msa0817120:intron 40.0%
TCCCATAGACAAACACTTGT+GGG - chr5_3:51963323-51963342 None:intergenic 40.0%
TTCCCATAGACAAACACTTG+TGG - chr5_3:51963324-51963343 None:intergenic 40.0%
! AAGCATATTGGTGGATCAGA+TGG + chr5_3:51963342-51963361 Msa0817120:intron 40.0%
TGATTGTATGCTTGAGACTG+AGG + chr5_3:51964192-51964211 Msa0817120:CDS 40.0%
GGAGAAGATGGTGATTCTGT+TGG - chr5_3:51962509-51962528 None:intergenic 45.0%
TACGTACGGGCAATAGGATT+TGG - chr5_3:51962530-51962549 None:intergenic 45.0%
!! GTTGTTGAGCTTGATTTGCG+AGG + chr5_3:51962685-51962704 Msa0817120:intron 45.0%
GTCCCACAAGTGTTTGTCTA+TGG + chr5_3:51963318-51963337 Msa0817120:intron 45.0%
! GTCTATGGGAAGCATATTGG+TGG + chr5_3:51963333-51963352 Msa0817120:intron 45.0%
AGGGATGCATGTTTGAGAGA+GGG - chr5_3:51963784-51963803 None:intergenic 45.0%
GTTTCTGCAATTCACCACTC+TGG - chr5_3:51964158-51964177 None:intergenic 45.0%
CGAAGGTTTCAAGCTTCTAG+CGG + chr5_3:51964270-51964289 Msa0817120:three_prime_UTR 45.0%
! GAAAGTACGATCATGAGCAG+TGG + chr5_3:51964406-51964425 Msa0817120:three_prime_UTR 45.0%
! AGCAGTGGAGCTCTAATTGT+TGG + chr5_3:51964421-51964440 Msa0817120:three_prime_UTR 45.0%
GAGGGATGCATGTTTGAGAG+AGG - chr5_3:51963785-51963804 None:intergenic 50.0%
CTCAGTGCTGCAGTCCAGAG+TGG + chr5_3:51964141-51964160 Msa0817120:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 51962280 51964784 51962280 ID=Msa0817120;Name=Msa0817120
chr5_3 mRNA 51962280 51964784 51962280 ID=Msa0817120-mRNA-1;Parent=Msa0817120;Name=Msa0817120-mRNA-1;_AED=0.21;_eAED=0.21;_QI=102|0.5|0.66|1|0|0.66|3|562|98
chr5_3 exon 51962280 51962542 51962280 ID=Msa0817120-mRNA-1:exon:15606;Parent=Msa0817120-mRNA-1
chr5_3 exon 51962633 51962684 51962633 ID=Msa0817120-mRNA-1:exon:15607;Parent=Msa0817120-mRNA-1
chr5_3 exon 51964139 51964784 51964139 ID=Msa0817120-mRNA-1:exon:15608;Parent=Msa0817120-mRNA-1
chr5_3 five_prime_UTR 51962280 51962381 51962280 ID=Msa0817120-mRNA-1:five_prime_utr;Parent=Msa0817120-mRNA-1
chr5_3 CDS 51962382 51962542 51962382 ID=Msa0817120-mRNA-1:cds;Parent=Msa0817120-mRNA-1
chr5_3 CDS 51962633 51962684 51962633 ID=Msa0817120-mRNA-1:cds;Parent=Msa0817120-mRNA-1
chr5_3 CDS 51964139 51964222 51964139 ID=Msa0817120-mRNA-1:cds;Parent=Msa0817120-mRNA-1
chr5_3 three_prime_UTR 51964223 51964784 51964223 ID=Msa0817120-mRNA-1:three_prime_utr;Parent=Msa0817120-mRNA-1
Gene Sequence

>Msa0817120

ATGAAGATGTTACAGTGGAGCATGTTCATAACTCTCACGTTATTGACCGTTTTACCCTTTTTGTTTGAGAATGGAACCGAAGCTTCAAATTCAGTTTCCGCTTTTGTTCAAAACGCAATCTACTCCAACAGAATCACCATCTTCTCCAAATCCTATTGCCCGTATTGTTTACGTGCTAAACGTGTTTTTGTTGAACTAAATGAGCAACCTTTTATCTCAGTGCTGCAGTCCAGAGTGGTGAATTGCAGAAACTTCTCAAATCAAGTTGATTGTATGCTTGAGACTGAGGAGCACTAG

Protein sequence

>Msa0817120

MKMLQWSMFITLTLLTVLPFLFENGTEASNSVSAFVQNAIYSNRITIFSKSYCPYCLRAKRVFVELNEQPFISVLQSRVVNCRNFSNQVDCMLETEEH*