AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32656


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32656.t1 MTR_3g106740 98.462 130 2 0 1 130 1 130 1.83e-85 245
MS.gene32656.t1 MTR_1g012690 59.091 154 37 3 1 129 1 153 9.54e-51 158
MS.gene32656.t1 MTR_1g012700 57.317 164 34 3 1 129 1 163 1.26e-50 158
MS.gene32656.t1 MTR_3g106750 73.282 131 30 2 1 129 1 128 1.63e-48 152
MS.gene32656.t1 MTR_1g012710 59.868 152 38 2 1 130 1 151 8.63e-48 150
MS.gene32656.t1 MTR_1g012630 78.571 84 17 1 46 129 127 209 4.82e-40 133
MS.gene32656.t1 MTR_4g108150 52.459 122 53 2 8 129 6 122 5.54e-35 117
MS.gene32656.t1 MTR_4g101260 51.969 127 55 3 8 129 6 131 7.44e-34 115
MS.gene32656.t1 MTR_2g026760 60.360 111 40 4 21 129 18 126 1.19e-33 114
MS.gene32656.t1 MTR_4g101280 63.218 87 30 2 44 129 57 142 2.53e-31 108
MS.gene32656.t1 MTR_4g101330 63.953 86 29 2 45 129 84 168 3.38e-30 107
MS.gene32656.t1 MTR_4g101310 60.000 85 32 2 46 129 50 133 2.55e-29 103
MS.gene32656.t1 MTR_4g101270 58.824 85 32 3 47 130 105 187 1.03e-23 90.9
MS.gene32656.t1 MTR_5g012340 44.828 87 45 2 44 129 92 176 8.45e-23 88.2
MS.gene32656.t1 MTR_5g012380 43.678 87 46 2 44 129 85 169 3.64e-22 86.7
MS.gene32656.t1 MTR_2g026920 45.679 81 41 2 50 130 118 195 2.45e-17 74.3
MS.gene32656.t1 MTR_2g026930 46.250 80 40 2 50 129 122 198 2.47e-17 74.7
MS.gene32656.t1 MTR_3g082100 44.828 87 47 1 44 130 251 336 1.60e-15 71.6
MS.gene32656.t1 MTR_2g026775 48.718 78 34 3 54 129 686 759 2.15e-15 71.6
MS.gene32656.t1 MTR_4g115360 54.023 87 37 3 44 129 148 232 5.27e-13 63.5
MS.gene32656.t1 MTR_2g026820 52.113 71 29 3 52 121 542 608 1.85e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32656.t1 AT2G45180 74.419 86 21 1 44 129 49 133 3.27e-37 123
MS.gene32656.t1 AT1G62510 68.235 85 25 2 46 129 66 149 7.74e-33 113
MS.gene32656.t1 AT4G12490 41.758 182 52 4 1 129 1 181 7.86e-32 111
MS.gene32656.t1 AT4G12480 64.368 87 29 2 44 129 82 167 1.01e-31 110
MS.gene32656.t1 AT4G12470 61.111 90 33 2 42 130 73 161 1.75e-31 110
MS.gene32656.t1 AT5G46890 54.962 131 52 5 1 129 1 126 1.89e-31 108
MS.gene32656.t1 AT4G12500 64.368 87 29 2 44 129 91 176 2.45e-31 110
MS.gene32656.t1 AT5G46900 69.663 89 23 3 41 129 42 126 2.66e-31 108
MS.gene32656.t1 AT1G12090 68.293 82 24 2 49 129 57 137 3.57e-31 108
MS.gene32656.t1 AT4G00165 54.808 104 43 3 29 129 26 128 5.86e-31 107
MS.gene32656.t1 AT4G00165 54.808 104 43 3 29 129 26 128 5.86e-31 107
MS.gene32656.t1 AT4G12520 54.198 131 55 5 1 129 1 128 2.93e-30 105
MS.gene32656.t1 AT4G12510 54.198 131 55 5 1 129 1 128 2.93e-30 105
MS.gene32656.t1 AT4G22460 51.852 108 52 0 23 130 26 133 3.48e-29 103
MS.gene32656.t1 AT4G12550 56.977 86 35 2 45 129 26 110 2.27e-28 100
MS.gene32656.t1 AT1G12100 43.651 126 65 4 7 129 9 131 3.60e-27 98.2
MS.gene32656.t1 AT4G12545 56.180 89 34 3 42 129 23 107 7.49e-26 94.0
MS.gene32656.t1 AT4G12530 47.126 87 45 1 44 130 31 116 9.77e-23 86.3
MS.gene32656.t1 AT3G22142 56.977 86 32 4 47 130 1397 1479 1.04e-22 92.8
MS.gene32656.t1 AT2G10940 55.172 87 36 3 44 129 205 289 1.32e-21 87.4
MS.gene32656.t1 AT2G10940 55.172 87 36 3 44 129 205 289 1.32e-21 87.4
MS.gene32656.t1 AT3G22120 49.412 85 41 2 47 130 267 350 1.48e-18 80.1
MS.gene32656.t1 AT4G15160 50.000 86 40 3 47 130 99 183 1.74e-18 77.4
MS.gene32656.t1 AT3G22120 49.412 85 41 2 47 130 250 333 2.54e-18 79.3
MS.gene32656.t1 AT4G15160 50.000 86 40 3 47 130 181 265 5.52e-18 77.8
MS.gene32656.t1 AT4G22490 37.500 96 53 3 37 130 27 117 6.63e-16 68.9
MS.gene32656.t1 AT1G62500 43.529 85 45 3 47 130 212 294 6.34e-15 69.7

Find 42 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATGCTGTGCATTGATTA+AGG 0.212710 3.1:+84593056 MS.gene32656:CDS
AGCACAAACCCCTAGCTTTA+AGG 0.218235 3.1:-84592983 None:intergenic
GGGTTGGTTAATGTTGTTAT+TGG 0.240421 3.1:+84593015 MS.gene32656:CDS
TCCTTAGGGCATTGACCTTT+GGG 0.253417 3.1:-84592958 None:intergenic
ATCCTTAGGGCATTGACCTT+TGG 0.263717 3.1:-84592959 None:intergenic
GATTAAGGGTTTGGCTGATT+TGG 0.309202 3.1:+84593071 MS.gene32656:CDS
AAGAGAAAGCACTAAAATTA+AGG 0.315935 3.1:-84592854 None:intergenic
TTGTCTTTGCACTGCACTTA+AGG 0.338630 3.1:+84593104 MS.gene32656:CDS
CCTAGCTTTAAGGTATCCTT+AGG 0.341849 3.1:-84592973 None:intergenic
AAATGCTGTGCATTGATTAA+GGG 0.392685 3.1:+84593057 MS.gene32656:CDS
CTGTGCATTGATTAAGGGTT+TGG 0.411029 3.1:+84593062 MS.gene32656:CDS
GAAGGTGTTGGCTTTGGTGA+TGG 0.414445 3.1:-84592913 None:intergenic
CCTTTGGGTGAAGGTGGAAC+AGG 0.427685 3.1:-84592943 None:intergenic
GTTATTGGAAACCCTCCTTC+AGG 0.428430 3.1:+84593030 MS.gene32656:CDS
AGGAGCACGCTGAGCGTGAT+AGG 0.432511 3.1:-84593156 None:intergenic
GGGCATTGACCTTTGGGTGA+AGG 0.436950 3.1:-84592952 None:intergenic
AATTAAGGCAGAGAATTTGT+TGG 0.438270 3.1:-84592839 None:intergenic
GCAGGTGAAGGTGTTGGCTT+TGG 0.458002 3.1:-84592919 None:intergenic
TAAGGGTTTGGCTGATTTGG+AGG 0.464987 3.1:+84593074 MS.gene32656:CDS
ATTGGAATCCTTAGGGACAT+TGG 0.481064 3.1:-84593212 None:intergenic
GGTGAAGGTGGAACAGGAGC+AGG 0.481606 3.1:-84592937 None:intergenic
GCACTTAAGGCTAATGTGCT+TGG 0.487972 3.1:+84593117 MS.gene32656:CDS
CCTGGTTTCCAATGTCCCTA+AGG 0.488513 3.1:+84593204 MS.gene32656:CDS
CCTAAGGATACCTTAAAGCT+AGG 0.505679 3.1:+84592973 MS.gene32656:CDS
CTAAGGATACCTTAAAGCTA+GGG 0.512185 3.1:+84592974 MS.gene32656:CDS
GGTGTTGGCTTTGGTGATGG+TGG 0.522065 3.1:-84592910 None:intergenic
CATTGACCTTTGGGTGAAGG+TGG 0.538821 3.1:-84592949 None:intergenic
CCTTAGGGACATTGGAAACC+AGG 0.543402 3.1:-84593204 None:intergenic
ACATTGGAAACCAGGAGGGA+CGG 0.567925 3.1:-84593196 None:intergenic
CCTGTTCCACCTTCACCCAA+AGG 0.572122 3.1:+84592943 MS.gene32656:CDS
CTAGCTTTAAGGTATCCTTA+GGG 0.574702 3.1:-84592972 None:intergenic
ACAGGAGCAGGTGAAGGTGT+TGG 0.576951 3.1:-84592925 None:intergenic
TGATGGTGGACAATTAGCTT+CGG 0.580942 3.1:-84592896 None:intergenic
ACCCAAAGGTCAATGCCCTA+AGG 0.586732 3.1:+84592957 MS.gene32656:CDS
GGTGGAACAGGAGCAGGTGA+AGG 0.595926 3.1:-84592931 None:intergenic
AGCTTCGGTGAATGTTGAGT+AGG 0.595958 3.1:-84592881 None:intergenic
AGCATTTACTACCTGAAGGA+GGG 0.596627 3.1:-84593041 None:intergenic
CAGCATTTACTACCTGAAGG+AGG 0.605436 3.1:-84593042 None:intergenic
TAAGGATACCTTAAAGCTAG+GGG 0.646982 3.1:+84592975 MS.gene32656:CDS
GCACAGCATTTACTACCTGA+AGG 0.650957 3.1:-84593045 None:intergenic
AGGGACATTGGAAACCAGGA+GGG 0.669232 3.1:-84593200 None:intergenic
TAGGGACATTGGAAACCAGG+AGG 0.715268 3.1:-84593201 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAGAAAGCACTAAAATTA+AGG - chr3.1:84592857-84592876 None:intergenic 25.0%
AATTAAGGCAGAGAATTTGT+TGG - chr3.1:84592842-84592861 None:intergenic 30.0%
!! AAATGCTGTGCATTGATTAA+GGG + chr3.1:84593057-84593076 MS.gene32656:CDS 30.0%
!! TAAATGCTGTGCATTGATTA+AGG + chr3.1:84593056-84593075 MS.gene32656:CDS 30.0%
CTAAGGATACCTTAAAGCTA+GGG + chr3.1:84592974-84592993 MS.gene32656:CDS 35.0%
TAAGGATACCTTAAAGCTAG+GGG + chr3.1:84592975-84592994 MS.gene32656:CDS 35.0%
!! CTAGCTTTAAGGTATCCTTA+GGG - chr3.1:84592975-84592994 None:intergenic 35.0%
!! GGGTTGGTTAATGTTGTTAT+TGG + chr3.1:84593015-84593034 MS.gene32656:CDS 35.0%
AGCATTTACTACCTGAAGGA+GGG - chr3.1:84593044-84593063 None:intergenic 40.0%
CCTAAGGATACCTTAAAGCT+AGG + chr3.1:84592973-84592992 MS.gene32656:CDS 40.0%
GATTAAGGGTTTGGCTGATT+TGG + chr3.1:84593071-84593090 MS.gene32656:CDS 40.0%
TTGTCTTTGCACTGCACTTA+AGG + chr3.1:84593104-84593123 MS.gene32656:CDS 40.0%
!! CCTAGCTTTAAGGTATCCTT+AGG - chr3.1:84592976-84592995 None:intergenic 40.0%
!! CTGTGCATTGATTAAGGGTT+TGG + chr3.1:84593062-84593081 MS.gene32656:CDS 40.0%
!! GGGTTTGTGCTGATCTTTTA+GGG + chr3.1:84592995-84593014 MS.gene32656:CDS 40.0%
!! TGATGGTGGACAATTAGCTT+CGG - chr3.1:84592899-84592918 None:intergenic 40.0%
!!! CGGTTTTTTGACATGCACTA+AGG - chr3.1:84593179-84593198 None:intergenic 40.0%
!!! TTGTGCTGATCTTTTAGGGT+TGG + chr3.1:84592999-84593018 MS.gene32656:CDS 40.0%
AGCTTCGGTGAATGTTGAGT+AGG - chr3.1:84592884-84592903 None:intergenic 45.0%
CAGCATTTACTACCTGAAGG+AGG - chr3.1:84593045-84593064 None:intergenic 45.0%
GCACAGCATTTACTACCTGA+AGG - chr3.1:84593048-84593067 None:intergenic 45.0%
GCACTTAAGGCTAATGTGCT+TGG + chr3.1:84593117-84593136 MS.gene32656:CDS 45.0%
GTTATTGGAAACCCTCCTTC+AGG + chr3.1:84593030-84593049 MS.gene32656:CDS 45.0%
!! AGCACAAACCCCTAGCTTTA+AGG - chr3.1:84592986-84593005 None:intergenic 45.0%
!! ATCCTTAGGGCATTGACCTT+TGG - chr3.1:84592962-84592981 None:intergenic 45.0%
!! GGGGTTTGTGCTGATCTTTT+AGG + chr3.1:84592994-84593013 MS.gene32656:CDS 45.0%
!! TAAGGGTTTGGCTGATTTGG+AGG + chr3.1:84593074-84593093 MS.gene32656:CDS 45.0%
!! TCCTTAGGGCATTGACCTTT+GGG - chr3.1:84592961-84592980 None:intergenic 45.0%
ACATTGGAAACCAGGAGGGA+CGG - chr3.1:84593199-84593218 None:intergenic 50.0%
ACCCAAAGGTCAATGCCCTA+AGG + chr3.1:84592957-84592976 MS.gene32656:CDS 50.0%
AGGGACATTGGAAACCAGGA+GGG - chr3.1:84593203-84593222 None:intergenic 50.0%
CATTGACCTTTGGGTGAAGG+TGG - chr3.1:84592952-84592971 None:intergenic 50.0%
TAGGGACATTGGAAACCAGG+AGG - chr3.1:84593204-84593223 None:intergenic 50.0%
TGTCAAAAAACCGTCCCTCC+TGG + chr3.1:84593186-84593205 MS.gene32656:CDS 50.0%
!! GAAGGTGTTGGCTTTGGTGA+TGG - chr3.1:84592916-84592935 None:intergenic 50.0%
CCTGTTCCACCTTCACCCAA+AGG + chr3.1:84592943-84592962 MS.gene32656:CDS 55.0%
CCTTTGGGTGAAGGTGGAAC+AGG - chr3.1:84592946-84592965 None:intergenic 55.0%
! ACAGGAGCAGGTGAAGGTGT+TGG - chr3.1:84592928-84592947 None:intergenic 55.0%
!! GCAGGTGAAGGTGTTGGCTT+TGG - chr3.1:84592922-84592941 None:intergenic 55.0%
!! GGGCATTGACCTTTGGGTGA+AGG - chr3.1:84592955-84592974 None:intergenic 55.0%
!! GGTGTTGGCTTTGGTGATGG+TGG - chr3.1:84592913-84592932 None:intergenic 55.0%
GGTGAAGGTGGAACAGGAGC+AGG - chr3.1:84592940-84592959 None:intergenic 60.0%
GGTGGAACAGGAGCAGGTGA+AGG - chr3.1:84592934-84592953 None:intergenic 60.0%
! AGGAGCACGCTGAGCGTGAT+AGG - chr3.1:84593159-84593178 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 84592832 84593224 84592832 ID=MS.gene32656
chr3.1 mRNA 84592832 84593224 84592832 ID=MS.gene32656.t1;Parent=MS.gene32656
chr3.1 exon 84592832 84593224 84592832 ID=MS.gene32656.t1.exon1;Parent=MS.gene32656.t1
chr3.1 CDS 84592832 84593224 84592832 ID=cds.MS.gene32656.t1;Parent=MS.gene32656.t1
Gene Sequence

>MS.gene32656

ATGGCTTCCAACAAATTCTCTGCCTTAATTTTAGTGCTTTCTCTTCTTGCCTACTCAACATTCACCGAAGCTAATTGTCCACCATCACCAAAGCCAACACCTTCACCTGCTCCTGTTCCACCTTCACCCAAAGGTCAATGCCCTAAGGATACCTTAAAGCTAGGGGTTTGTGCTGATCTTTTAGGGTTGGTTAATGTTGTTATTGGAAACCCTCCTTCAGGTAGTAAATGCTGTGCATTGATTAAGGGTTTGGCTGATTTGGAGGCTGCACTTTGTCTTTGCACTGCACTTAAGGCTAATGTGCTTGGAATCAACTTGAACGTGCCTATCACGCTCAGCGTGCTCCTTAGTGCATGTCAAAAAACCGTCCCTCCTGGTTTCCAATGTCCCTAA

Protein sequence

>MS.gene32656.t1

MASNKFSALILVLSLLAYSTFTEANCPPSPKPTPSPAPVPPSPKGQCPKDTLKLGVCADLLGLVNVVIGNPPSGSKCCALIKGLADLEAALCLCTALKANVLGINLNVPITLSVLLSACQKTVPPGFQCP