AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41633


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41633.t1 MTR_3g098490 96.682 211 7 0 1 211 1 211 4.51e-155 428
MS.gene41633.t1 MTR_3g098500 76.037 217 44 3 1 211 7 221 1.74e-119 338
MS.gene41633.t1 MTR_8g096110 68.617 188 56 2 1 186 1 187 4.51e-93 271
MS.gene41633.t1 MTR_8g096080 70.000 190 52 3 1 186 1 189 1.29e-92 270
MS.gene41633.t1 MTR_8g096100 60.199 201 77 3 1 199 1 200 1.16e-82 245
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41633.t1 AT3G23760 63.684 190 61 3 7 190 7 194 4.87e-86 253
MS.gene41633.t1 AT4G14100 63.978 186 57 3 14 191 7 190 9.43e-85 250

Find 61 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATGGTGCTACCTATTT+AGG 0.222154 3.4:+88360060 MS.gene41633:CDS
CTCCGTCCTAATTGGCTTGA+TGG 0.262701 3.4:+88360045 MS.gene41633:CDS
AAGCGGATCCCTACAGAAAT+TGG 0.272446 3.4:+88359858 MS.gene41633:CDS
TCCGGCCAAGCTGCCGGAAC+TGG 0.316253 3.4:-88359805 None:intergenic
AACTTGTTCTTGACCTAAAT+AGG 0.332003 3.4:-88360073 None:intergenic
ATAATTTCCTATGTAATGTT+TGG 0.333466 3.4:+88360097 MS.gene41633:CDS
CCCTACAGAAATTGGACTTA+TGG 0.343249 3.4:+88359866 MS.gene41633:CDS
TGCAATAGAACACACCTTTA+CGG 0.348173 3.4:+88359735 MS.gene41633:CDS
ACTGTTCGTGGCGATTCTTT+TGG 0.354532 3.4:+88360665 MS.gene41633:CDS
CAACATCATTGAGTTGATCT+TGG 0.364611 3.4:-88359924 None:intergenic
TTGGTATTCTCCGTCCTAAT+TGG 0.369794 3.4:+88360037 MS.gene41633:CDS
GTAGCACCATCAAGCCAATT+AGG 0.370390 3.4:-88360051 None:intergenic
TTGTAGATTACGACCTTGAA+TGG 0.373551 3.4:+88359944 MS.gene41633:CDS
TAATTTCCTATGTAATGTTT+GGG 0.374401 3.4:+88360098 MS.gene41633:CDS
CTCGACGGCGGAGCCAGTTC+CGG 0.389600 3.4:+88359792 MS.gene41633:CDS
GCCAGTTCCGGCAGCTTGGC+CGG 0.421754 3.4:+88359804 MS.gene41633:CDS
GTGTAGAAGATCCACTTAAC+AGG 0.439368 3.4:-88360168 None:intergenic
AACTGCTCCGGCCAAGCTGC+CGG 0.446630 3.4:-88359811 None:intergenic
TTAGAATCTGCTACTGTTCG+TGG 0.447496 3.4:+88360653 MS.gene41633:CDS
ATGTTGAAGTTTCGACCGTT+GGG 0.451295 3.4:-88359898 None:intergenic
GTCTGATTGAAGCAGTATAC+AGG 0.464973 3.4:-88360620 None:intergenic
AAGTAGTACCGTTACCGTAA+AGG 0.468001 3.4:-88359749 None:intergenic
TGCAGTGGCGGTCGGAGGGA+TGG 0.474901 3.4:-88359996 None:intergenic
AGGTTCCATTGGTCCATTCA+AGG 0.477181 3.4:-88359957 None:intergenic
CTTTCTCCCAAACATTACAT+AGG 0.489592 3.4:-88360104 None:intergenic
GATGTTGAAGTTTCGACCGT+TGG 0.491687 3.4:-88359899 None:intergenic
CAAAGTCGATGCTGCTGCAT+AGG 0.503520 3.4:+88360703 MS.gene41633:CDS
CGGAGCCAGTTCCGGCAGCT+TGG 0.510708 3.4:+88359800 MS.gene41633:CDS
AACCTTGCAGTGGCGGTCGG+AGG 0.511934 3.4:-88360001 None:intergenic
ACCTTGCAGTGGCGGTCGGA+GGG 0.512978 3.4:-88360000 None:intergenic
ACCATAAGTCCAATTTCTGT+AGG 0.527172 3.4:-88359867 None:intergenic
TCACTCGAAGGCCTGTTAAG+TGG 0.539058 3.4:+88360157 MS.gene41633:CDS
GACAACCTTGCAGTGGCGGT+CGG 0.543354 3.4:-88360004 None:intergenic
TGCTCATGTGATGACATTTG+AGG 0.548966 3.4:+88360562 MS.gene41633:CDS
GTTAAGTGGATCTTCTACAC+AGG 0.549287 3.4:+88360171 MS.gene41633:CDS
GCTTGAGGATGTGCATTGGC+AGG 0.549400 3.4:+88360595 MS.gene41633:CDS
TCCCTCCGACCGCCACTGCA+AGG 0.553601 3.4:+88359999 MS.gene41633:CDS
GTACATGAAGGAGGTTCCAT+TGG 0.555646 3.4:-88359968 None:intergenic
GCTTCTGTTCATGAATAGTA+CGG 0.566725 3.4:-88359839 None:intergenic
CCATAAGTCCAATTTCTGTA+GGG 0.573008 3.4:-88359866 None:intergenic
CATGGACAACCTTGCAGTGG+CGG 0.573319 3.4:-88360008 None:intergenic
AGTACGGAATGAAACTGCTC+CGG 0.575238 3.4:-88359823 None:intergenic
AATTGGACTTATGGTACGAC+TGG 0.582011 3.4:+88359875 MS.gene41633:CDS
TTATGGTACGACTGGCCCAA+CGG 0.591091 3.4:+88359883 MS.gene41633:CDS
AGAACACACCTTTACGGTAA+CGG 0.593520 3.4:+88359741 MS.gene41633:CDS
CATGTGATGACATTTGAGGT+TGG 0.595333 3.4:+88360566 MS.gene41633:CDS
AAGGTTGTCCATGTTGAAGT+TGG 0.595989 3.4:+88360018 MS.gene41633:CDS
ATGAAGATGTTCTCACTCGA+AGG 0.605789 3.4:+88360145 MS.gene41633:CDS
CACCATCAAGCCAATTAGGA+CGG 0.609769 3.4:-88360047 None:intergenic
TGAGGTTGGAGCAGTGCTTG+AGG 0.612681 3.4:+88360580 MS.gene41633:CDS
TACGACCTTGAATGGACCAA+TGG 0.624357 3.4:+88359952 MS.gene41633:CDS
AAAGGTGTGTTCTATTGCAG+AGG 0.630711 3.4:-88359731 None:intergenic
GTACTATTCATGAACAGAAG+CGG 0.639978 3.4:+88359841 MS.gene41633:CDS
GGGATGGAGAGTGTACATGA+AGG 0.647111 3.4:-88359980 None:intergenic
CAGTGCTTGAGGATGTGCAT+TGG 0.659088 3.4:+88360591 MS.gene41633:CDS
ATGGAGAGTGTACATGAAGG+AGG 0.663920 3.4:-88359977 None:intergenic
GGAGAATACCAACTTCAACA+TGG 0.664515 3.4:-88360026 None:intergenic
TCTCTTCTCACTCTCGACGG+CGG 0.673727 3.4:+88359780 MS.gene41633:CDS
CAACATGGACAACCTTGCAG+TGG 0.677545 3.4:-88360011 None:intergenic
CGTTCTCTTCTCACTCTCGA+CGG 0.680397 3.4:+88359777 MS.gene41633:CDS
CGAGAGTGAGAAGAGAACGA+CGG 0.733666 3.4:-88359773 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTTCCTATGTAATGTT+TGG + chr3.4:88360097-88360116 MS.gene41633:CDS 20.0%
!! ATGATAAACTAATAAGTTCA+AGG - chr3.4:88360426-88360445 None:intergenic 20.0%
!! CTTATATGATAACAATTTGT+CGG + chr3.4:88360319-88360338 MS.gene41633:intron 20.0%
!! TAATTTCCTATGTAATGTTT+GGG + chr3.4:88360098-88360117 MS.gene41633:CDS 20.0%
!! TGATAAACTAATAAGTTCAA+GGG - chr3.4:88360425-88360444 None:intergenic 20.0%
! TGAATTAAGGTTTGATATAC+TGG + chr3.4:88360520-88360539 MS.gene41633:intron 25.0%
!!! TTTGGATTCATTGACTTATT+TGG + chr3.4:88360239-88360258 MS.gene41633:intron 25.0%
AATCCAAACAAACAGTAACT+AGG - chr3.4:88360227-88360246 None:intergenic 30.0%
CAAACCTTAATTCATTTCAC+AGG - chr3.4:88360514-88360533 None:intergenic 30.0%
CATTCCTGTGAAATGAATTA+AGG + chr3.4:88360507-88360526 MS.gene41633:intron 30.0%
GATATACTGGTAATTAATGC+AGG + chr3.4:88360533-88360552 MS.gene41633:intron 30.0%
! AACTTGTTCTTGACCTAAAT+AGG - chr3.4:88360076-88360095 None:intergenic 30.0%
ACCATAAGTCCAATTTCTGT+AGG - chr3.4:88359870-88359889 None:intergenic 35.0%
ACTCCTAGTTACTGTTTGTT+TGG + chr3.4:88360221-88360240 MS.gene41633:intron 35.0%
CCATAAGTCCAATTTCTGTA+GGG - chr3.4:88359869-88359888 None:intergenic 35.0%
CTTTCTCCCAAACATTACAT+AGG - chr3.4:88360107-88360126 None:intergenic 35.0%
GCTTCTGTTCATGAATAGTA+CGG - chr3.4:88359842-88359861 None:intergenic 35.0%
GTACTATTCATGAACAGAAG+CGG + chr3.4:88359841-88359860 MS.gene41633:CDS 35.0%
TGCAATAGAACACACCTTTA+CGG + chr3.4:88359735-88359754 MS.gene41633:CDS 35.0%
TTGTAGATTACGACCTTGAA+TGG + chr3.4:88359944-88359963 MS.gene41633:CDS 35.0%
!! CAACATCATTGAGTTGATCT+TGG - chr3.4:88359927-88359946 None:intergenic 35.0%
!! TCTTTTGGAAGTTTGATGAG+AGG + chr3.4:88360680-88360699 MS.gene41633:CDS 35.0%
AAGGTTGTCCATGTTGAAGT+TGG + chr3.4:88360018-88360037 MS.gene41633:CDS 40.0%
AAGTAGTACCGTTACCGTAA+AGG - chr3.4:88359752-88359771 None:intergenic 40.0%
AATTGGACTTATGGTACGAC+TGG + chr3.4:88359875-88359894 MS.gene41633:CDS 40.0%
AGAACACACCTTTACGGTAA+CGG + chr3.4:88359741-88359760 MS.gene41633:CDS 40.0%
ATGAAGATGTTCTCACTCGA+AGG + chr3.4:88360145-88360164 MS.gene41633:CDS 40.0%
ATGTTGAAGTTTCGACCGTT+GGG - chr3.4:88359901-88359920 None:intergenic 40.0%
CCCTACAGAAATTGGACTTA+TGG + chr3.4:88359866-88359885 MS.gene41633:CDS 40.0%
GGAGAATACCAACTTCAACA+TGG - chr3.4:88360029-88360048 None:intergenic 40.0%
GTCTGATTGAAGCAGTATAC+AGG - chr3.4:88360623-88360642 None:intergenic 40.0%
GTGTAGAAGATCCACTTAAC+AGG - chr3.4:88360171-88360190 None:intergenic 40.0%
GTTAAGTGGATCTTCTACAC+AGG + chr3.4:88360171-88360190 MS.gene41633:CDS 40.0%
TGCTCATGTGATGACATTTG+AGG + chr3.4:88360562-88360581 MS.gene41633:CDS 40.0%
TTAGAATCTGCTACTGTTCG+TGG + chr3.4:88360653-88360672 MS.gene41633:CDS 40.0%
TTGGTATTCTCCGTCCTAAT+TGG + chr3.4:88360037-88360056 MS.gene41633:CDS 40.0%
! AAAGGTGTGTTCTATTGCAG+AGG - chr3.4:88359734-88359753 None:intergenic 40.0%
! CATGTGATGACATTTGAGGT+TGG + chr3.4:88360566-88360585 MS.gene41633:CDS 40.0%
!! CTTGATGGTGCTACCTATTT+AGG + chr3.4:88360060-88360079 MS.gene41633:CDS 40.0%
AAGCGGATCCCTACAGAAAT+TGG + chr3.4:88359858-88359877 MS.gene41633:CDS 45.0%
AGGTTCCATTGGTCCATTCA+AGG - chr3.4:88359960-88359979 None:intergenic 45.0%
AGTACGGAATGAAACTGCTC+CGG - chr3.4:88359826-88359845 None:intergenic 45.0%
CACCATCAAGCCAATTAGGA+CGG - chr3.4:88360050-88360069 None:intergenic 45.0%
GATGTTGAAGTTTCGACCGT+TGG - chr3.4:88359902-88359921 None:intergenic 45.0%
GTACATGAAGGAGGTTCCAT+TGG - chr3.4:88359971-88359990 None:intergenic 45.0%
GTAGCACCATCAAGCCAATT+AGG - chr3.4:88360054-88360073 None:intergenic 45.0%
TACGACCTTGAATGGACCAA+TGG + chr3.4:88359952-88359971 MS.gene41633:CDS 45.0%
! ACTGTTCGTGGCGATTCTTT+TGG + chr3.4:88360665-88360684 MS.gene41633:CDS 45.0%
! ATGGAGAGTGTACATGAAGG+AGG - chr3.4:88359980-88359999 None:intergenic 45.0%
CAACATGGACAACCTTGCAG+TGG - chr3.4:88360014-88360033 None:intergenic 50.0%
CAGTGCTTGAGGATGTGCAT+TGG + chr3.4:88360591-88360610 MS.gene41633:CDS 50.0%
CGAGAGTGAGAAGAGAACGA+CGG - chr3.4:88359776-88359795 None:intergenic 50.0%
CGTTCTCTTCTCACTCTCGA+CGG + chr3.4:88359777-88359796 MS.gene41633:CDS 50.0%
TCACTCGAAGGCCTGTTAAG+TGG + chr3.4:88360157-88360176 MS.gene41633:CDS 50.0%
! GGGATGGAGAGTGTACATGA+AGG - chr3.4:88359983-88360002 None:intergenic 50.0%
! TTATGGTACGACTGGCCCAA+CGG + chr3.4:88359883-88359902 MS.gene41633:CDS 50.0%
!! CTCCGTCCTAATTGGCTTGA+TGG + chr3.4:88360045-88360064 MS.gene41633:CDS 50.0%
CATGGACAACCTTGCAGTGG+CGG - chr3.4:88360011-88360030 None:intergenic 55.0%
TCTCTTCTCACTCTCGACGG+CGG + chr3.4:88359780-88359799 MS.gene41633:CDS 55.0%
TGAGGTTGGAGCAGTGCTTG+AGG + chr3.4:88360580-88360599 MS.gene41633:CDS 55.0%
! GCTTGAGGATGTGCATTGGC+AGG + chr3.4:88360595-88360614 MS.gene41633:CDS 55.0%
GACAACCTTGCAGTGGCGGT+CGG - chr3.4:88360007-88360026 None:intergenic 60.0%
AACCTTGCAGTGGCGGTCGG+AGG - chr3.4:88360004-88360023 None:intergenic 65.0%
AACTGCTCCGGCCAAGCTGC+CGG - chr3.4:88359814-88359833 None:intergenic 65.0%
ACCTTGCAGTGGCGGTCGGA+GGG - chr3.4:88360003-88360022 None:intergenic 65.0%
CGGAGCCAGTTCCGGCAGCT+TGG + chr3.4:88359800-88359819 MS.gene41633:CDS 70.0%
CTCGACGGCGGAGCCAGTTC+CGG + chr3.4:88359792-88359811 MS.gene41633:CDS 70.0%
GCCAGTTCCGGCAGCTTGGC+CGG + chr3.4:88359804-88359823 MS.gene41633:CDS 70.0%
TCCCTCCGACCGCCACTGCA+AGG + chr3.4:88359999-88360018 MS.gene41633:CDS 70.0%
TCCGGCCAAGCTGCCGGAAC+TGG - chr3.4:88359808-88359827 None:intergenic 70.0%
TGCAGTGGCGGTCGGAGGGA+TGG - chr3.4:88359999-88360018 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 88359727 88360724 88359727 ID=MS.gene41633
chr3.4 mRNA 88359727 88360724 88359727 ID=MS.gene41633.t1;Parent=MS.gene41633
chr3.4 exon 88359727 88360192 88359727 ID=MS.gene41633.t1.exon1;Parent=MS.gene41633.t1
chr3.4 CDS 88359727 88360192 88359727 ID=cds.MS.gene41633.t1;Parent=MS.gene41633.t1
chr3.4 exon 88360555 88360724 88360555 ID=MS.gene41633.t1.exon2;Parent=MS.gene41633.t1
chr3.4 CDS 88360555 88360724 88360555 ID=cds.MS.gene41633.t1;Parent=MS.gene41633.t1
Gene Sequence

>MS.gene41633

ATGGCCTCTGCAATAGAACACACCTTTACGGTAACGGTACTACTTTCCGTCGTTCTCTTCTCACTCTCGACGGCGGAGCCAGTTCCGGCAGCTTGGCCGGAGCAGTTTCATTCCGTACTATTCATGAACAGAAGCGGATCCCTACAGAAATTGGACTTATGGTACGACTGGCCCAACGGTCGAAACTTCAACATCATCCAAGATCAACTCAATGATGTTGTAGATTACGACCTTGAATGGACCAATGGAACCTCCTTCATGTACACTCTCCATCCCTCCGACCGCCACTGCAAGGTTGTCCATGTTGAAGTTGGTATTCTCCGTCCTAATTGGCTTGATGGTGCTACCTATTTAGGTCAAGAACAAGTTGATAATTTCCTATGTAATGTTTGGGAGAAAGTTGATTTCATTGTGTATTATGAAGATGTTCTCACTCGAAGGCCTGTTAAGTGGATCTTCTACACAGGTTTGTTTACTTCTTTCTTTCTAATATCACTCCTAGTTACTGTTTGTTTGGATTCATTGACTTATTTGGAAGAGTTTATAGAAACAGCTTATTACATGTCAATGAGTTTGTGAAAATAGCTTATAGCTTATATGATAACAATTTGTCGGTATAAAACTATTTGATTTTATTTCTTGTAATATAAAAAACTTGTACATATCACTTGTATAGAAAGAGTTTATGCTGTAAGCCCTTGAACTTATTAGTTTATCATTTTCAAGAGATTGAATGAATGAGAATAAATTTATGATATTATTATTCTTTGAAATGTTGTTCATTCCTGTGAAATGAATTAAGGTTTGATATACTGGTAATTAATGCAGGATACACTGCTCATGTGATGACATTTGAGGTTGGAGCAGTGCTTGAGGATGTGCATTGGCAGGCTCCTGTATACTGCTTCAATCAGACTGAGAATATGTTAGAATCTGCTACTGTTCGTGGCGATTCTTTTGGAAGTTTGATGAGAGGCAAAGTCGATGCTGCTGCATAG

Protein sequence

>MS.gene41633.t1

MASAIEHTFTVTVLLSVVLFSLSTAEPVPAAWPEQFHSVLFMNRSGSLQKLDLWYDWPNGRNFNIIQDQLNDVVDYDLEWTNGTSFMYTLHPSDRHCKVVHVEVGILRPNWLDGATYLGQEQVDNFLCNVWEKVDFIVYYEDVLTRRPVKWIFYTGYTAHVMTFEVGAVLEDVHWQAPVYCFNQTENMLESATVRGDSFGSLMRGKVDAAA