AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56284


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56284.t1 MTR_3g010000 91.358 162 2 1 1 162 1 150 1.43e-104 296
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56284.t1 AT1G55670 70.303 165 41 3 1 162 1 160 1.38e-77 228

Find 23 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTATGATCCAAGTTTCTT+TGG 0.278269 3.1:-5149241 MS.gene56284:CDS
TTGCTCAGGTAAGCCTTGTT+TGG 0.325198 3.1:+5149423 None:intergenic
TATTGTTGCTTACTATATCT+TGG 0.334380 3.1:-5149278 MS.gene56284:CDS
GTTAAAACCAACAGGATCAT+TGG 0.338510 3.1:+5149336 None:intergenic
CCTTGGAAGGACAAGCTTGA+TGG 0.371656 3.1:+5149622 None:intergenic
CAAGGCTTACCTGAGCAAAA+TGG 0.416510 3.1:-5149418 MS.gene56284:CDS
TCAACAAGGTTAAAACCAAC+AGG 0.475786 3.1:+5149328 None:intergenic
CCATCAAGCTTGTCCTTCCA+AGG 0.477711 3.1:-5149622 MS.gene56284:CDS
TTGTGAGAGGGTTGAAACCT+TGG 0.480579 3.1:+5149605 None:intergenic
CTTAAATCCAATGATCCTGT+TGG 0.489692 3.1:-5149343 MS.gene56284:CDS
CTTAAGCTTATCACAAGTGA+AGG 0.506695 3.1:+5149508 None:intergenic
AAATATTAACCAAAGAAACT+TGG 0.511509 3.1:+5149232 None:intergenic
AGCTGGTGACAAACGTGCTA+AGG 0.525842 3.1:-5149380 MS.gene56284:CDS
TAATGCATGAGTTGATATCA+TGG 0.550417 3.1:+5149684 None:intergenic
ATCTTGGCTACTTCTAGCAA+TGG 0.561786 3.1:-5149262 MS.gene56284:CDS
AGAGAAAATGTAGCCAAACA+AGG 0.605177 3.1:-5149436 MS.gene56284:CDS
CTTGTGATAAGCTTAAGCAC+TGG 0.608219 3.1:-5149502 MS.gene56284:CDS
AAGGTTTCAACCCTCTCACA+AGG 0.615424 3.1:-5149603 MS.gene56284:CDS
GAGAGGGTTGAAACCTTGGA+AGG 0.623355 3.1:+5149609 None:intergenic
TTAAGCTTATCACAAGTGAA+GGG 0.638403 3.1:+5149509 None:intergenic
ATGAGTTGATATCATGGATG+AGG 0.640889 3.1:+5149690 None:intergenic
GGTGTGACACACTTTGAAGC+TGG 0.654419 3.1:-5149397 MS.gene56284:CDS
CCCAAGCCAAAACATCAACA+AGG 0.663918 3.1:+5149314 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTTGCTTACTATATCT+TGG - chr3.1:5149662-5149681 MS.gene56284:CDS 25.0%
!! TTTTAACCTTGTTGATGTTT+TGG - chr3.1:5149620-5149639 MS.gene56284:CDS 25.0%
TTAAGCTTATCACAAGTGAA+GGG + chr3.1:5149434-5149453 None:intergenic 30.0%
!! AGTTTTGTGTTTTTGAATGG+TGG + chr3.1:5149286-5149305 None:intergenic 30.0%
!! GTGAGTTTTGTGTTTTTGAA+TGG + chr3.1:5149289-5149308 None:intergenic 30.0%
!! TAATGCATGAGTTGATATCA+TGG + chr3.1:5149259-5149278 None:intergenic 30.0%
!!! ACTGGTCTTTCATTGTTTTT+AGG - chr3.1:5149456-5149475 MS.gene56284:CDS 30.0%
!!! CTTGAAAACCTTTTTTTGCT+TGG + chr3.1:5149387-5149406 None:intergenic 30.0%
!!! GTCTTTCATTGTTTTTAGGA+AGG - chr3.1:5149460-5149479 MS.gene56284:CDS 30.0%
AGAGAAAATGTAGCCAAACA+AGG - chr3.1:5149504-5149523 MS.gene56284:CDS 35.0%
CATTCACACCAAGCAAAAAA+AGG - chr3.1:5149376-5149395 MS.gene56284:CDS 35.0%
CTTAAATCCAATGATCCTGT+TGG - chr3.1:5149597-5149616 MS.gene56284:CDS 35.0%
CTTAAGCTTATCACAAGTGA+AGG + chr3.1:5149435-5149454 None:intergenic 35.0%
GGTTATGATCCAAGTTTCTT+TGG - chr3.1:5149699-5149718 MS.gene56284:CDS 35.0%
GTTAAAACCAACAGGATCAT+TGG + chr3.1:5149607-5149626 None:intergenic 35.0%
TCAACAAGGTTAAAACCAAC+AGG + chr3.1:5149615-5149634 None:intergenic 35.0%
! GTTTGGCTACATTTTCTCTT+TGG + chr3.1:5149503-5149522 None:intergenic 35.0%
!! ATGAGTTGATATCATGGATG+AGG + chr3.1:5149253-5149272 None:intergenic 35.0%
ATCTTGGCTACTTCTAGCAA+TGG - chr3.1:5149678-5149697 MS.gene56284:CDS 40.0%
CTTGTGATAAGCTTAAGCAC+TGG - chr3.1:5149438-5149457 MS.gene56284:CDS 40.0%
! ACTTTTGTTGACCTTGTGAG+AGG + chr3.1:5149351-5149370 None:intergenic 40.0%
! CTTTTGTTGACCTTGTGAGA+GGG + chr3.1:5149350-5149369 None:intergenic 40.0%
!!! ACCTTGTTGATGTTTTGGCT+TGG - chr3.1:5149625-5149644 MS.gene56284:CDS 40.0%
!!! CCTTGTTGATGTTTTGGCTT+GGG - chr3.1:5149626-5149645 MS.gene56284:CDS 40.0%
!!! CTTGTTGATGTTTTGGCTTG+GGG - chr3.1:5149627-5149646 MS.gene56284:CDS 40.0%
AAGGTTTCAACCCTCTCACA+AGG - chr3.1:5149337-5149356 MS.gene56284:CDS 45.0%
CAAGGCTTACCTGAGCAAAA+TGG - chr3.1:5149522-5149541 MS.gene56284:CDS 45.0%
CCCAAGCCAAAACATCAACA+AGG + chr3.1:5149629-5149648 None:intergenic 45.0%
TTGTGAGAGGGTTGAAACCT+TGG + chr3.1:5149338-5149357 None:intergenic 45.0%
! TGTGTCACACCATTTTGCTC+AGG + chr3.1:5149534-5149553 None:intergenic 45.0%
!! TTGCTCAGGTAAGCCTTGTT+TGG + chr3.1:5149520-5149539 None:intergenic 45.0%
!!! GTTTTGGCTTGGGGTTCTTT+AGG - chr3.1:5149636-5149655 MS.gene56284:CDS 45.0%
AGCTGGTGACAAACGTGCTA+AGG - chr3.1:5149560-5149579 MS.gene56284:CDS 50.0%
CCATCAAGCTTGTCCTTCCA+AGG - chr3.1:5149318-5149337 MS.gene56284:CDS 50.0%
CCTTGGAAGGACAAGCTTGA+TGG + chr3.1:5149321-5149340 None:intergenic 50.0%
GAGAGGGTTGAAACCTTGGA+AGG + chr3.1:5149334-5149353 None:intergenic 50.0%
!! GGTGTGACACACTTTGAAGC+TGG - chr3.1:5149543-5149562 MS.gene56284:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 5149237 5149725 5149237 ID=MS.gene56284
chr3.1 mRNA 5149237 5149725 5149237 ID=MS.gene56284.t1;Parent=MS.gene56284
chr3.1 exon 5149237 5149725 5149237 ID=MS.gene56284.t1.exon1;Parent=MS.gene56284.t1
chr3.1 CDS 5149237 5149725 5149237 ID=cds.MS.gene56284.t1;Parent=MS.gene56284.t1
Gene Sequence

>MS.gene56284

ATGGCAGCTTCAGCCTCATCCATGATATCAACTCATGCATTAACAACCACCATTCAAAAACACAAAACTCACAATCTCAAGCCATCAAGCTTGTCCTTCCAAGGTTTCAACCCTCTCACAAGGTCAACAAAAGTCAACACATTCACACCAAGCAAAAAAAGGTTTTCAAGTGTTGTTGTCAAAGCTGAACTCAACCCTTCACTTGTGATAAGCTTAAGCACTGGTCTTTCATTGTTTTTAGGAAGGTTTGTGTTTTTCAACTTCCAAAGAGAAAATGTAGCCAAACAAGGCTTACCTGAGCAAAATGGTGTGACACACTTTGAAGCTGGTGACAAACGTGCTAAGGAATATGTTAGCTTGCTTAAATCCAATGATCCTGTTGGTTTTAACCTTGTTGATGTTTTGGCTTGGGGTTCTTTAGGTCATATTGTTGCTTACTATATCTTGGCTACTTCTAGCAATGGTTATGATCCAAGTTTCTTTGGTTAA

Protein sequence

>MS.gene56284.t1

MAASASSMISTHALTTTIQKHKTHNLKPSSLSFQGFNPLTRSTKVNTFTPSKKRFSSVVVKAELNPSLVISLSTGLSLFLGRFVFFNFQRENVAKQGLPEQNGVTHFEAGDKRAKEYVSLLKSNDPVGFNLVDVLAWGSLGHIVAYYILATSSNGYDPSFFG