AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0325550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTTGGATGTGTAATATTA+AGG 0.194324 2_4:-33178130 Msa0325550:three_prime_UTR
CCATTGATTTACAATAAATA+AGG 0.239329 2_4:+33178094 None:intergenic
CTGAAAAGAAGCTTCAATTT+AGG 0.255528 2_4:-33178375 Msa0325550:CDS
ATCCCTCCTCTTTGTTAAAC+TGG 0.287597 2_4:+33178268 None:intergenic
CCTTATTTATTGTAAATCAA+TGG 0.342117 2_4:-33178094 Msa0325550:three_prime_UTR
AAGAAAAGATGAAAAGTGTA+TGG 0.395163 2_4:-33178233 Msa0325550:three_prime_UTR
GTACTGTACCTGATACAATA+TGG 0.402588 2_4:+33178401 None:intergenic
CTTATTTATTGTAAATCAAT+GGG 0.402734 2_4:-33178093 Msa0325550:three_prime_UTR
GTGAAAGATAAGAAAGATCT+TGG 0.408538 2_4:-33178318 Msa0325550:three_prime_UTR
GAGGAGAACCATATTGTATC+AGG 0.416982 2_4:-33178409 Msa0325550:CDS
TCTTTCTTATCTTTCACCTT+GGG 0.419428 2_4:+33178324 None:intergenic
ATCTTTCTTATCTTTCACCT+TGG 0.466513 2_4:+33178323 None:intergenic
GTTTAACAAAGAGGAGGGAT+TGG 0.488431 2_4:-33178265 Msa0325550:three_prime_UTR
CAATATGGTTCTCCTCTTCA+CGG 0.490112 2_4:+33178416 None:intergenic
GGTGAAATATGAAAGAGGAT+TGG 0.494887 2_4:-33182067 None:intergenic
TTTCCGTTCCTCCACTCTCA+AGG 0.526382 2_4:+33182027 Msa0325560:five_prime_UTR
GAATATCTTGAGGAATATGT+TGG 0.532265 2_4:-33178441 Msa0325550:CDS
AGAAAAGATGAAAAGTGTAT+GGG 0.540681 2_4:-33178232 Msa0325550:three_prime_UTR
TGAGCCACTATATCATGTTG+TGG 0.552754 2_4:+33178204 None:intergenic
AGGACCACAACATGATATAG+TGG 0.561954 2_4:-33178208 Msa0325550:three_prime_UTR
TACCAGTTTAACAAAGAGGA+GGG 0.568079 2_4:-33178270 Msa0325550:three_prime_UTR
ATAAAGGTGAAATATGAAAG+AGG 0.587394 2_4:-33182072 None:intergenic
GTATTACCAGTTTAACAAAG+AGG 0.598288 2_4:-33178274 Msa0325550:three_prime_UTR
AGAACGAGATACCTTGAGAG+TGG 0.599631 2_4:-33182038 Msa0325550:CDS
TCTTGGAATCATGAAAGTCA+AGG 0.609272 2_4:-33178301 Msa0325550:three_prime_UTR
TTACCAGTTTAACAAAGAGG+AGG 0.617647 2_4:-33178271 Msa0325550:three_prime_UTR
ATACCTTGAGAGTGGAGGAA+CGG 0.630198 2_4:-33182030 Msa0325550:CDS
TCACGTGCAGGAATATCTTG+AGG 0.635000 2_4:-33178451 Msa0325550:intron
AAGATGAAAAGTGTATGGGA+AGG 0.640402 2_4:-33178228 Msa0325550:three_prime_UTR
AATATGTTGGAACCGTGAAG+AGG 0.650450 2_4:-33178428 Msa0325550:CDS
GTCATGACTTGATATACCCA+AGG 0.651402 2_4:-33178340 Msa0325550:exon
ACGAGATACCTTGAGAGTGG+AGG 0.668379 2_4:-33182035 Msa0325550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAATTAAGAAGTAA+CGG - chr2_4:33178986-33179005 Msa0325550:intron 10.0%
!!! TTAAAAGGAATTATTTTATT+AGG - chr2_4:33179106-33179125 Msa0325550:intron 10.0%
!!! TAAAAGGAATTATTTTATTA+GGG - chr2_4:33179107-33179126 Msa0325550:intron 10.0%
!! TATTAATAATATTATTGTTC+GGG - chr2_4:33179676-33179695 Msa0325550:intron 10.0%
!!! GAATTTTAGTTAAAATAATA+AGG + chr2_4:33180088-33180107 None:intergenic 10.0%
!!! CTTTTAATTTTTTTTTTTGA+AGG + chr2_4:33180593-33180612 None:intergenic 10.0%
!! TAATAGATAAGATTAAAAAA+AGG + chr2_4:33181023-33181042 None:intergenic 10.0%
!! ATAGATAAGATTAAAAAAAA+AGG + chr2_4:33181151-33181170 None:intergenic 10.0%
!!! TTTAAGTTTTTTTTTTCATT+CGG + chr2_4:33181235-33181254 None:intergenic 10.0%
!!! TTTCAGTAAATTTACAATTT+TGG + chr2_4:33178680-33178699 None:intergenic 15.0%
!! AATTGTAAATTTACTGAAAT+AGG - chr2_4:33178681-33178700 Msa0325550:intron 15.0%
!! ATTGTAAATTTACTGAAATA+GGG - chr2_4:33178682-33178701 Msa0325550:intron 15.0%
!!! AATTTTTTAAAAAAACCGTT+TGG + chr2_4:33178727-33178746 None:intergenic 15.0%
!!! ATCTAAATTTTATTCCAAAT+TGG + chr2_4:33178947-33178966 None:intergenic 15.0%
!!! AAAAGGAATTATTTTATTAG+GGG - chr2_4:33179108-33179127 Msa0325550:intron 15.0%
!! AATGCTATTAAAATTAAGTA+GGG + chr2_4:33179589-33179608 None:intergenic 15.0%
!! AAATGCTATTAAAATTAAGT+AGG + chr2_4:33179588-33179607 None:intergenic 15.0%
!! AGATAAAAAGTAATAAAATC+CGG + chr2_4:33179374-33179393 None:intergenic 15.0%
!! TTCAAAAATTTAAGAGTTTA+AGG - chr2_4:33179505-33179524 Msa0325550:intron 15.0%
!! AAATGCTATTAAAATTAAGT+AGG - chr2_4:33179588-33179607 Msa0325550:intron 15.0%
!! AATGCTATTAAAATTAAGTA+GGG - chr2_4:33179589-33179608 Msa0325550:intron 15.0%
!! TAAAATAATAAGGGTAAAAA+TGG + chr2_4:33180078-33180097 None:intergenic 15.0%
!! CATATTTACCTTTATAATTT+AGG - chr2_4:33180107-33180126 Msa0325550:intron 15.0%
!! CATTTAAACCTAAATTATAA+AGG + chr2_4:33180118-33180137 None:intergenic 15.0%
!! TTTGTGTTAAAAATTACATT+TGG - chr2_4:33180646-33180665 Msa0325550:intron 15.0%
!!! TTTTTCCATTAAAATTGATT+TGG + chr2_4:33180720-33180739 None:intergenic 15.0%
!! TCTTAAATATTTATATCATC+CGG - chr2_4:33181313-33181332 Msa0325550:intron 15.0%
!!! AATTGGTATAAATTGTTTTA+AGG - chr2_4:33181463-33181482 Msa0325550:intron 15.0%
!!! TATCATTTTTCATTTATCTT+TGG + chr2_4:33181500-33181519 None:intergenic 15.0%
!! CTTATTTATTGTAAATCAAT+GGG - chr2_4:33182044-33182063 Msa0325550:CDS 15.0%
!!! ATTTTAGTAATCTGCAATTT+TGG - chr2_4:33178505-33178524 Msa0325550:intron 20.0%
!!! TCTAAGGTTTTAAAAATAAG+GGG + chr2_4:33178581-33178600 None:intergenic 20.0%
!!! GTCTAAGGTTTTAAAAATAA+GGG + chr2_4:33178582-33178601 None:intergenic 20.0%
!!! TGTCTAAGGTTTTAAAAATA+AGG + chr2_4:33178583-33178602 None:intergenic 20.0%
!!! TTTAAAACCTTAGACATTAT+AGG - chr2_4:33178587-33178606 Msa0325550:intron 20.0%
!! TTAAAACCTTAGACATTATA+GGG - chr2_4:33178588-33178607 Msa0325550:intron 20.0%
!! AAAAAAAAATCTTGGACATT+TGG + chr2_4:33178634-33178653 None:intergenic 20.0%
!! TTGTAAATTTACTGAAATAG+GGG - chr2_4:33178683-33178702 Msa0325550:intron 20.0%
!! AAAATTGAATTTAAGCCAAA+CGG - chr2_4:33178709-33178728 Msa0325550:intron 20.0%
!!! TAAATTTTATTCCAAATTGG+AGG + chr2_4:33178944-33178963 None:intergenic 20.0%
!! AAACAAATTTGAATTGAGTA+GGG + chr2_4:33179244-33179263 None:intergenic 20.0%
!! TAAACAAATTTGAATTGAGT+AGG + chr2_4:33179245-33179264 None:intergenic 20.0%
!!! AATTTTGCGATAATTTTTTG+CGG + chr2_4:33179339-33179358 None:intergenic 20.0%
!!! ATACAAAAAAATGTGAGTTT+TGG - chr2_4:33179737-33179756 Msa0325550:intron 20.0%
!! GTCATAAAATTGTCATAAAA+TGG - chr2_4:33179823-33179842 Msa0325550:intron 20.0%
!! AAAAATAAGTTATAAACTCG+TGG + chr2_4:33180005-33180024 None:intergenic 20.0%
!!! TTTAGGTTTAAATGTGTAAT+TGG - chr2_4:33180124-33180143 Msa0325550:intron 20.0%
!!! CTTTGAAAAAATATTGGTAT+TGG - chr2_4:33180275-33180294 Msa0325550:intron 20.0%
!!! CTTTGAAAAAATATTGGTAT+TGG - chr2_4:33180275-33180294 Msa0325550:intron 20.0%
!! CTTTGTAAAAATATTGGTAT+TGG + chr2_4:33180389-33180408 None:intergenic 20.0%
!! TATAGGTTTAAATGTGTAAT+TGG + chr2_4:33180539-33180558 None:intergenic 20.0%
!! TAAAATGTTATAATAGTGAC+CGG + chr2_4:33181335-33181354 None:intergenic 20.0%
!! TCATGTTAACATAAAAGATT+AGG - chr2_4:33181604-33181623 Msa0325550:intron 20.0%
!!! CCATTGATTTACAATAAATA+AGG + chr2_4:33182046-33182065 Msa0325560:intron 20.0%
!!! CCTTATTTATTGTAAATCAA+TGG - chr2_4:33182043-33182062 Msa0325550:CDS 20.0%
!!! GTTTGGCTTAAATTCAATTT+TGG + chr2_4:33178710-33178729 None:intergenic 25.0%
!! TAATTGTAGTACTTTGCTTT+TGG - chr2_4:33178889-33178908 Msa0325550:intron 25.0%
!!! CAAGTTTTTTTGTTGAGTTT+CGG - chr2_4:33178917-33178936 Msa0325550:intron 25.0%
! AGTGCAGAAAAACAAAATTA+GGG + chr2_4:33179211-33179230 None:intergenic 25.0%
!! CAAAATTTGATTTCTACATC+CGG - chr2_4:33179352-33179371 Msa0325550:intron 25.0%
!!! TAATCAAACTTTGTTTTAGC+AGG - chr2_4:33179533-33179552 Msa0325550:intron 25.0%
!!! AATCAAACTTTGTTTTAGCA+GGG - chr2_4:33179534-33179553 Msa0325550:intron 25.0%
!! TTTGTTATCAAAGGCTTATT+CGG + chr2_4:33179882-33179901 None:intergenic 25.0%
!!! CAATATTTTTACAAAGTGCA+GGG + chr2_4:33180393-33180412 None:intergenic 25.0%
!!! CAATATTTTTTCAAAGTGCA+GGG + chr2_4:33180271-33180290 None:intergenic 25.0%
!!! CAATATTTTTTCAAAGTGCA+GGG + chr2_4:33180271-33180290 None:intergenic 25.0%
!! CTTTGGAAAAATATTGGTAT+TGG + chr2_4:33180358-33180377 None:intergenic 25.0%
!!! CAATATTTTTACAAAGTGCA+GGG - chr2_4:33180393-33180412 Msa0325550:intron 25.0%
!!! TACTTTTTTTTGTTTGGCAT+TGG + chr2_4:33180420-33180439 None:intergenic 25.0%
! CTTTGTAAAAATATTGGCAT+TGG + chr2_4:33180215-33180234 None:intergenic 25.0%
!!! CAATATTTTTACAAAGTGCT+GGG - chr2_4:33180453-33180472 Msa0325550:intron 25.0%
!! AGAGTCCAAATCAATTTTAA+TGG - chr2_4:33180712-33180731 Msa0325550:intron 25.0%
!! AAAGACATTAGCAATTTTAG+AGG + chr2_4:33180772-33180791 None:intergenic 25.0%
! GAGAACTAATATAAAAGTCA+CGG - chr2_4:33180798-33180817 Msa0325550:intron 25.0%
! AATGAGTTTGAGAACAAAAT+TGG + chr2_4:33180859-33180878 None:intergenic 25.0%
!! GAGAGAAACAAAAGTATATA+CGG + chr2_4:33180970-33180989 None:intergenic 25.0%
! CATTCATTAAATGATGTATG+TGG - chr2_4:33181359-33181378 Msa0325550:intron 25.0%
! TAATAACCAAGTTGTGTAAT+TGG - chr2_4:33181446-33181465 Msa0325550:intron 25.0%
! TTTATACCAATTACACAACT+TGG + chr2_4:33181455-33181474 None:intergenic 25.0%
!! AAATTTTATATATCACGTGC+AGG - chr2_4:33181674-33181693 Msa0325550:intron 25.0%
!! AAGAAAAGATGAAAAGTGTA+TGG - chr2_4:33181904-33181923 Msa0325550:intron 25.0%
!! AGAAAAGATGAAAAGTGTAT+GGG - chr2_4:33181905-33181924 Msa0325550:intron 25.0%
!!! TTCTTTGTAATGTTTTGACT+TGG - chr2_4:33181991-33182010 Msa0325550:CDS 25.0%
AATTTAACAAACCCAAACGT+GGG + chr2_4:33178241-33178260 None:intergenic 30.0%
!! ACGTTTGGGTTTGTTAAATT+GGG - chr2_4:33178241-33178260 Msa0325550:three_prime_UTR 30.0%
AAGATATAATGGTCGTTTAC+TGG + chr2_4:33178357-33178376 None:intergenic 30.0%
AACGACCATTATATCTTCAA+CGG - chr2_4:33178360-33178379 Msa0325550:CDS 30.0%
! ATAATGGTCTTTTACTTACG+TGG - chr2_4:33178428-33178447 Msa0325550:CDS 30.0%
! TAATGGTCTTTTACTTACGT+GGG - chr2_4:33178429-33178448 Msa0325550:CDS 30.0%
AAATAAGGGGACTAAAATTG+CGG + chr2_4:33178568-33178587 None:intergenic 30.0%
TATAGGGTGACTAAATTCAA+TGG - chr2_4:33178604-33178623 Msa0325550:intron 30.0%
! AGCAAAGTACTACAATTACA+AGG + chr2_4:33178887-33178906 None:intergenic 30.0%
AACTCAACAAAAAAACTTGC+AGG + chr2_4:33178916-33178935 None:intergenic 30.0%
!! TACAAGGTCGCTATTTTATA+GGG + chr2_4:33179620-33179639 None:intergenic 30.0%
!! TTACAAGGTCGCTATTTTAT+AGG + chr2_4:33179619-33179638 None:intergenic 30.0%
CAGTGCAGAAAAACAAAATT+AGG + chr2_4:33179212-33179231 None:intergenic 30.0%
CAAGATTAGAACACTATGAA+AGG + chr2_4:33179471-33179490 None:intergenic 30.0%
!! TTACAAGGTCGCTATTTTAT+AGG - chr2_4:33179619-33179638 Msa0325550:intron 30.0%
!! TACAAGGTCGCTATTTTATA+GGG - chr2_4:33179620-33179639 Msa0325550:intron 30.0%
! AGCCTTTGATAACAAAAGTA+AGG - chr2_4:33179886-33179905 Msa0325550:intron 30.0%
! ACCAAGAAATTAGCTTATAG+CGG - chr2_4:33179925-33179944 Msa0325550:intron 30.0%
!!! CACTTTTTTTTGTTTGGCAT+TGG - chr2_4:33180184-33180203 Msa0325550:intron 30.0%
!! CCAATATTTTTACAAAGTGC+AGG + chr2_4:33180392-33180411 None:intergenic 30.0%
CCTGCACTTTGTAAAAATAT+TGG - chr2_4:33180209-33180228 Msa0325550:intron 30.0%
!!! CACTTTTTTTTGTTTGGCAT+TGG - chr2_4:33180184-33180203 Msa0325550:intron 30.0%
!!! CCAATATTTTTTCAAAGTGC+AGG + chr2_4:33180272-33180291 None:intergenic 30.0%
! CCTGCACTTTGAAAAAATAT+TGG - chr2_4:33180269-33180288 Msa0325550:intron 30.0%
!!! CCAATATTTTTTCAAAGTGC+AGG + chr2_4:33180272-33180291 None:intergenic 30.0%
! CCTGCACTTTGAAAAAATAT+TGG - chr2_4:33180269-33180288 Msa0325550:intron 30.0%
! CAATATTTTTCCAAAGTGCA+GGG - chr2_4:33180362-33180381 Msa0325550:intron 30.0%
CCTGCACTTTGTAAAAATAT+TGG + chr2_4:33180209-33180228 None:intergenic 30.0%
!! CCAATATTTTTACAAAGTGC+AGG - chr2_4:33180392-33180411 Msa0325550:intron 30.0%
! TGCCAAACAAAAAAAAGTAC+AGG - chr2_4:33180421-33180440 Msa0325550:intron 30.0%
! GCCAAACAAAAAAAAGTACA+GGG - chr2_4:33180422-33180441 Msa0325550:intron 30.0%
!!! TCCCTGTACTTTTTTTTGTT+TGG + chr2_4:33180426-33180445 None:intergenic 30.0%
CCAGCACTTTGTAAAAATAT+TGG + chr2_4:33180455-33180474 None:intergenic 30.0%
!! CCAATATTTTTACAAAGTGC+TGG - chr2_4:33180392-33180411 Msa0325550:intron 30.0%
TGTGTTAATGTGCTATCTTT+AGG - chr2_4:33181636-33181655 Msa0325550:intron 30.0%
GAATATCTTGAGGAATATGT+TGG - chr2_4:33181696-33181715 Msa0325550:intron 30.0%
CTGAAAAGAAGCTTCAATTT+AGG - chr2_4:33181762-33181781 Msa0325550:intron 30.0%
TCTTTCTTATCTTTCACCTT+GGG + chr2_4:33181816-33181835 None:intergenic 30.0%
ATCTTTCTTATCTTTCACCT+TGG + chr2_4:33181817-33181836 None:intergenic 30.0%
GTGAAAGATAAGAAAGATCT+TGG - chr2_4:33181819-33181838 Msa0325550:intron 30.0%
GTATTACCAGTTTAACAAAG+AGG - chr2_4:33181863-33181882 Msa0325550:intron 30.0%
GACTTGGATGTGTAATATTA+AGG - chr2_4:33182007-33182026 Msa0325550:CDS 30.0%
!! AAAAAGTGACGGGAGTTTTA+GGG - chr2_4:33178130-33178149 Msa0325550:three_prime_UTR 35.0%
CAATTTAACAAACCCAAACG+TGG + chr2_4:33178242-33178261 None:intergenic 35.0%
!! CACGTTTGGGTTTGTTAAAT+TGG - chr2_4:33178240-33178259 Msa0325550:three_prime_UTR 35.0%
!! TTAGGCCCATTAAGTTGAAA+GGG + chr2_4:33178267-33178286 None:intergenic 35.0%
GAACTCCGTTGAAGATATAA+TGG + chr2_4:33178368-33178387 None:intergenic 35.0%
AGTCAATTGTGTATGTGAGA+AGG - chr2_4:33178453-33178472 Msa0325550:intron 35.0%
GTCAATTGTGTATGTGAGAA+GGG - chr2_4:33178454-33178473 Msa0325550:intron 35.0%
GGTTAGTAACTCTGCAATTT+CGG - chr2_4:33178526-33178545 Msa0325550:intron 35.0%
TAGTCACCCTATAATGTCTA+AGG + chr2_4:33178597-33178616 None:intergenic 35.0%
TCCAATCGTTTCATATCATG+TGG - chr2_4:33179019-33179038 Msa0325550:intron 35.0%
AAGTAGGGACTAAACTTACA+AGG + chr2_4:33179604-33179623 None:intergenic 35.0%
AAGTAGGGACTAAACTTACA+AGG - chr2_4:33179604-33179623 Msa0325550:intron 35.0%
!! CGCCTTACTTTTGTTATCAA+AGG + chr2_4:33179891-33179910 None:intergenic 35.0%
TCCGCTATAAGCTAATTTCT+TGG + chr2_4:33179929-33179948 None:intergenic 35.0%
! CAAAATCTGTTGAAAGTGCA+GGG + chr2_4:33180512-33180531 None:intergenic 35.0%
!! CTTTCAACAGATTTTGGCAT+TGG - chr2_4:33180155-33180174 Msa0325550:intron 35.0%
!!! TCCCTGCACTTTTTTTTGTT+TGG - chr2_4:33180178-33180197 Msa0325550:intron 35.0%
! GCCAAACAAAAAAAAGTGCA+GGG + chr2_4:33180182-33180201 None:intergenic 35.0%
! TGCCAAACAAAAAAAAGTGC+AGG + chr2_4:33180183-33180202 None:intergenic 35.0%
!!! TCCCTGCACTTTTTTTTGTT+TGG - chr2_4:33180178-33180197 Msa0325550:intron 35.0%
! GCCAAACAAAAAAAAGTGCA+GGG + chr2_4:33180182-33180201 None:intergenic 35.0%
! TGCCAAACAAAAAAAAGTGC+AGG + chr2_4:33180183-33180202 None:intergenic 35.0%
! CCTGCACTTTGGAAAAATAT+TGG + chr2_4:33180364-33180383 None:intergenic 35.0%
! CCAATATTTTTCCAAAGTGC+AGG - chr2_4:33180361-33180380 Msa0325550:intron 35.0%
!!! GCACTTTTTTTGTTTGGCAT+TGG + chr2_4:33180480-33180499 None:intergenic 35.0%
! ATGCCAAACAAAAAAAGTGC+AGG - chr2_4:33180480-33180499 Msa0325550:intron 35.0%
! TGCCAAACAAAAAAAGTGCA+GGG - chr2_4:33180481-33180500 Msa0325550:intron 35.0%
!! CTTTCAACAGATTTTGGCAT+TGG + chr2_4:33180155-33180174 None:intergenic 35.0%
! CAAAATCTGTTGAAAGTGCA+GGG - chr2_4:33180512-33180531 Msa0325550:intron 35.0%
GTACTGTACCTGATACAATA+TGG + chr2_4:33181739-33181758 None:intergenic 35.0%
TCTTGGAATCATGAAAGTCA+AGG - chr2_4:33181836-33181855 Msa0325550:intron 35.0%
TTACCAGTTTAACAAAGAGG+AGG - chr2_4:33181866-33181885 Msa0325550:intron 35.0%
TACCAGTTTAACAAAGAGGA+GGG - chr2_4:33181867-33181886 Msa0325550:intron 35.0%
! AAGATGAAAAGTGTATGGGA+AGG - chr2_4:33181909-33181928 Msa0325550:intron 35.0%
!! GAAAAAGTGACGGGAGTTTT+AGG - chr2_4:33178129-33178148 Msa0325550:three_prime_UTR 40.0%
ATTGGGCCCTTTCAACTTAA+TGG - chr2_4:33178258-33178277 Msa0325550:three_prime_UTR 40.0%
TTGGGCCCTTTCAACTTAAT+GGG - chr2_4:33178259-33178278 Msa0325550:three_prime_UTR 40.0%
!! CTTAGGCCCATTAAGTTGAA+AGG + chr2_4:33178268-33178287 None:intergenic 40.0%
! AGCCAAAGCGTTTACAACTT+AGG + chr2_4:33178285-33178304 None:intergenic 40.0%
TAATGGTCGTTTACTGGTGT+GGG + chr2_4:33178351-33178370 None:intergenic 40.0%
ATAATGGTCGTTTACTGGTG+TGG + chr2_4:33178352-33178371 None:intergenic 40.0%
CTTCAACGGAGTTCTTATGT+CGG - chr2_4:33178374-33178393 Msa0325550:CDS 40.0%
TTCAACGGAGTTCTTATGTC+GGG - chr2_4:33178375-33178394 Msa0325550:CDS 40.0%
AGGGTGACTAAATTCAATGG+AGG - chr2_4:33178607-33178626 Msa0325550:intron 40.0%
GCCACATGATATGAAACGAT+TGG + chr2_4:33179023-33179042 None:intergenic 40.0%
CATATCATGTGGCACAATGA+AGG - chr2_4:33179030-33179049 Msa0325550:intron 40.0%
! CCAAAATCTGTTGAAAGTGC+AGG + chr2_4:33180511-33180530 None:intergenic 40.0%
! CCTGCACTTTCAACAGATTT+TGG - chr2_4:33180149-33180168 Msa0325550:intron 40.0%
!!! GTCCCTGCACTTTTTTTGTT+TGG + chr2_4:33180486-33180505 None:intergenic 40.0%
! CCTGCACTTTCAACAGATTT+TGG + chr2_4:33180149-33180168 None:intergenic 40.0%
! CCAAAATCTGTTGAAAGTGC+AGG - chr2_4:33180511-33180530 Msa0325550:intron 40.0%
ATTCCAGCTGTGTACATACT+AGG - chr2_4:33180824-33180843 Msa0325550:intron 40.0%
ATACCTAGTATGTACACAGC+TGG + chr2_4:33180830-33180849 None:intergenic 40.0%
GCTGTGTACATACTAGGTAT+TGG - chr2_4:33180830-33180849 Msa0325550:intron 40.0%
AATATGTTGGAACCGTGAAG+AGG - chr2_4:33181709-33181728 Msa0325550:intron 40.0%
CAATATGGTTCTCCTCTTCA+CGG + chr2_4:33181724-33181743 None:intergenic 40.0%
GAGGAGAACCATATTGTATC+AGG - chr2_4:33181728-33181747 Msa0325550:intron 40.0%
GTCATGACTTGATATACCCA+AGG - chr2_4:33181797-33181816 Msa0325550:intron 40.0%
ATCCCTCCTCTTTGTTAAAC+TGG + chr2_4:33181872-33181891 None:intergenic 40.0%
GTTTAACAAAGAGGAGGGAT+TGG - chr2_4:33181872-33181891 Msa0325550:intron 40.0%
AGGACCACAACATGATATAG+TGG - chr2_4:33181929-33181948 Msa0325550:intron 40.0%
TGAGCCACTATATCATGTTG+TGG + chr2_4:33181936-33181955 None:intergenic 40.0%
AGAACGAGATACCTTGAGAG+TGG - chr2_4:33178099-33178118 Msa0325550:three_prime_UTR 45.0%
ATACCTTGAGAGTGGAGGAA+CGG - chr2_4:33178107-33178126 Msa0325550:three_prime_UTR 45.0%
TGGAGGAACGGAAAAAGTGA+CGG - chr2_4:33178119-33178138 Msa0325550:three_prime_UTR 45.0%
TGTAACTTGATGCCCACGTT+TGG - chr2_4:33178226-33178245 Msa0325550:three_prime_UTR 45.0%
GTAACTTGATGCCCACGTTT+GGG - chr2_4:33178227-33178246 Msa0325550:three_prime_UTR 45.0%
GGCCTAAGTTGTAAACGCTT+TGG - chr2_4:33178280-33178299 Msa0325550:three_prime_UTR 45.0%
GAGTTCTTATGTCGGGAAGA+TGG - chr2_4:33178382-33178401 Msa0325550:CDS 45.0%
TTCTTATGTCGGGAAGATGG+CGG - chr2_4:33178385-33178404 Msa0325550:CDS 45.0%
TAACTGTCGCTGGGGTATAA+TGG - chr2_4:33178412-33178431 Msa0325550:CDS 45.0%
TGAGTTTCGGTCCTCCAATT+TGG - chr2_4:33178930-33178949 Msa0325550:intron 45.0%
!! AACAAAAGTAAGGCGCTGTC+TGG - chr2_4:33179896-33179915 Msa0325550:intron 45.0%
!! ATATTGGTATTGGTGCCACG+TGG - chr2_4:33180316-33180335 Msa0325550:intron 45.0%
CCACGTGGCATGAAATGATT+GGG - chr2_4:33180331-33180350 Msa0325550:intron 45.0%
TCACGTGCAGGAATATCTTG+AGG - chr2_4:33181686-33181705 Msa0325550:intron 45.0%
!!! TTTTTAAAAAAAAAAAATCT+TGG + chr2_4:33178642-33178661 None:intergenic 5.0%
!!! TTTTAAAAAAAAAAAAAAAG+TGG - chr2_4:33178653-33178672 Msa0325550:intron 5.0%
!!! AATATATATGAATTTTTATT+TGG - chr2_4:33178806-33178825 Msa0325550:intron 5.0%
!!! TATTGTTTTAATTTTTTAAA+AGG - chr2_4:33179091-33179110 Msa0325550:intron 5.0%
!! TAAAATTATAAGTAAATTAT+AGG - chr2_4:33179559-33179578 Msa0325550:intron 5.0%
!! AAAATTATAAGTAAATTATA+GGG - chr2_4:33179560-33179579 Msa0325550:intron 5.0%
!! ATTAATAATAACTAATAAAA+GGG + chr2_4:33179663-33179682 None:intergenic 5.0%
!! TATTAATAATAACTAATAAA+AGG + chr2_4:33179664-33179683 None:intergenic 5.0%
!! TTATTAATAATATTATTGTT+CGG - chr2_4:33179675-33179694 Msa0325550:intron 5.0%
!!! AATTTTAGTTAAAATAATAA+GGG + chr2_4:33180087-33180106 None:intergenic 5.0%
!!! TTTAAAGTATAATAAATTAT+AGG + chr2_4:33180556-33180575 None:intergenic 5.0%
!! AAAAAAAAAATTATATGAAA+TGG + chr2_4:33180905-33180924 None:intergenic 5.0%
ACGAGATACCTTGAGAGTGG+AGG - chr2_4:33178102-33178121 Msa0325550:three_prime_UTR 50.0%
TTTCCGTTCCTCCACTCTCA+AGG + chr2_4:33178113-33178132 None:intergenic 50.0%
GGAGGAACGGAAAAAGTGAC+GGG - chr2_4:33178120-33178139 Msa0325550:three_prime_UTR 50.0%
!!! GGGAGTTTTAGGGTTTTGCG+CGG - chr2_4:33178140-33178159 Msa0325550:three_prime_UTR 50.0%
GCCACGTGGCATGAAATGAT+TGG - chr2_4:33180330-33180349 Msa0325550:intron 50.0%
CCCAATCATTTCATGCCACG+TGG + chr2_4:33180334-33180353 None:intergenic 50.0%
!! TGGTATTGGTCCCTGCACTT+TGG + chr2_4:33180289-33180308 None:intergenic 50.0%
TGGCGGCAGTTAACTGTCGC+TGG - chr2_4:33178402-33178421 Msa0325550:CDS 60.0%
GGCGGCAGTTAACTGTCGCT+GGG - chr2_4:33178403-33178422 Msa0325550:CDS 60.0%
GCGGCAGTTAACTGTCGCTG+GGG - chr2_4:33178404-33178423 Msa0325550:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 33178078 33182081 33178078 ID=Msa0325550;Name=Msa0325550
chr2_4 mRNA 33178078 33182081 33178078 ID=Msa0325550-mRNA-1;Parent=Msa0325550;Name=Msa0325550-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0.5|0.5|0|0.5|2|278|69
chr2_4 exon 33178078 33178463 33178078 ID=Msa0325550-mRNA-1:exon:10728;Parent=Msa0325550-mRNA-1
chr2_4 exon 33181980 33182081 33181980 ID=Msa0325550-mRNA-1:exon:10727;Parent=Msa0325550-mRNA-1
chr2_4 CDS 33181980 33182081 33181980 ID=Msa0325550-mRNA-1:cds;Parent=Msa0325550-mRNA-1
chr2_4 CDS 33178356 33178463 33178356 ID=Msa0325550-mRNA-1:cds;Parent=Msa0325550-mRNA-1
chr2_4 three_prime_UTR 33178078 33178355 33178078 ID=Msa0325550-mRNA-1:three_prime_utr;Parent=Msa0325550-mRNA-1
Gene Sequence

>Msa0325550

ATGAAAGAGGATTGGAAGAGAAGAACGAGATACCTTGAGAGTGGAGGAACGGAAAAAGTGACGGGAGTTTTAGGGTTTTGCGCGGAAGAAGAGCAAGAATGTGAATATCTTGAGGAATATGTTGGAACCGTGAAGAGGAGAACCATATTGTATCAGGTACAGTACAAACTGAAAAGAAGCTTCAATTTAGGTTGTTGTCAATTGTCATGA
Protein sequence

>Msa0325550

MKEDWKRRTRYLESGGTEKVTGVLGFCAEEEQECEYLEEYVGTVKRRTILYQVQYKLKRSFNLGCCQLS*