AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0558840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0558840 MtrunA17_Chr8g0378681 97.980 99 2 0 2 100 236 334 3.88e-65 199
Msa0558840 MtrunA17_Chr4g0025631 93.939 99 6 0 2 100 279 377 4.17e-61 190
Msa0558840 MtrunA17_Chr1g0197131 83.838 99 16 0 2 100 35 133 9.44e-55 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0558840 AT5G38720.1 72.000 100 27 1 2 100 207 306 3.26e-45 148
Msa0558840 AT5G38720.3 72.000 100 27 1 2 100 214 313 3.32e-45 148
Msa0558840 AT5G38720.2 72.000 100 27 1 2 100 226 325 6.56e-45 147

Find 21 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGCTGCAGAAAATAAAT+TGG 0.319112 4_1:+57853430 Msa0558840:CDS
GCAATCAACATCATTTAATA+AGG 0.321323 4_1:-57854199 None:intergenic
AAAAGAGAAGCTCAGAGAAA+TGG 0.446445 4_1:+57853500 Msa0558840:CDS
TCCATCCTCCTTCTGCAGCA+AGG 0.468377 4_1:-57853331 None:intergenic
AAGTGGGATTGCTGTGGGCT+CGG 0.474663 4_1:+57853400 Msa0558840:CDS
CCATCCTCCTTCTGCAGCAA+GGG 0.496362 4_1:-57853330 None:intergenic
CGCTGAAAGTGGGATTGCTG+TGG 0.507910 4_1:+57853394 Msa0558840:CDS
AAAACTACTGACGCTGAAAG+TGG 0.530432 4_1:+57853383 Msa0558840:CDS
CCCTTGCTGCAGAAGGAGGA+TGG 0.545811 4_1:+57853330 Msa0558840:CDS
GGAACTGCAGAGCAAATTTG+AGG 0.547063 4_1:+57854129 Msa0558840:CDS
AAAGAAGCCCTTGCTGCAGA+AGG 0.553866 4_1:+57853323 Msa0558840:CDS
TGCTGCAGAAGGAGGATGGA+CGG 0.572937 4_1:+57853334 Msa0558840:CDS
GAAGCCCTTGCTGCAGAAGG+AGG 0.575071 4_1:+57853326 Msa0558840:CDS
AAACTACTGACGCTGAAAGT+GGG 0.579773 4_1:+57853384 Msa0558840:CDS
GCTGAAAGTGGGATTGCTGT+GGG 0.581833 4_1:+57853395 Msa0558840:CDS
GACGGTGGTTCAGCACCACA+AGG 0.611340 4_1:+57853352 Msa0558840:CDS
TGGTTCAGCACCACAAGGGT+AGG 0.619462 4_1:+57853357 Msa0558840:CDS
TGCAACAGTTGAGAGCTGCT+AGG 0.650823 4_1:+57854167 Msa0558840:CDS
ACTGCAGAGCAAATTTGAGG+AGG 0.684181 4_1:+57854132 Msa0558840:CDS
TGCAGAAGGAGGATGGACGG+TGG 0.709610 4_1:+57853337 Msa0558840:CDS
ACGGTGGTTCAGCACCACAA+GGG 0.735362 4_1:+57853353 Msa0558840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGTTTAAGAAATATTA+GGG + chr4_1:57853751-57853770 Msa0558840:intron 10.0%
!!! TTTTAGTTTAAGAAATATTA+GGG + chr4_1:57853751-57853770 Msa0558840:intron 10.0%
!!! CTTTTAGTTTAAGAAATATT+AGG + chr4_1:57853750-57853769 Msa0558840:intron 15.0%
!!! CTTTTAGTTTAAGAAATATT+AGG + chr4_1:57853750-57853769 Msa0558840:intron 15.0%
!!! TAAATTTAAACGAACAGTTT+TGG + chr4_1:57853631-57853650 Msa0558840:intron 20.0%
!!! AACAGTTTTGGTTTAAATAA+TGG + chr4_1:57853643-57853662 Msa0558840:intron 20.0%
!!! AATAATGGAAGATGTTAATT+AGG + chr4_1:57853658-57853677 Msa0558840:intron 20.0%
!!! ATAATGGAAGATGTTAATTA+GGG + chr4_1:57853659-57853678 Msa0558840:intron 20.0%
!! TTAAGAAATATTAGGGTTAT+TGG + chr4_1:57853758-57853777 Msa0558840:intron 20.0%
!!! AAATTATATGATTTTATGCG+AGG + chr4_1:57853970-57853989 Msa0558840:intron 20.0%
!!! TAAATTTAAACGAACAGTTT+TGG + chr4_1:57853631-57853650 Msa0558840:intron 20.0%
!!! AACAGTTTTGGTTTAAATAA+TGG + chr4_1:57853643-57853662 Msa0558840:intron 20.0%
!!! AATAATGGAAGATGTTAATT+AGG + chr4_1:57853658-57853677 Msa0558840:intron 20.0%
!!! ATAATGGAAGATGTTAATTA+GGG + chr4_1:57853659-57853678 Msa0558840:intron 20.0%
!! TTAAGAAATATTAGGGTTAT+TGG + chr4_1:57853758-57853777 Msa0558840:intron 20.0%
!!! AAATTATATGATTTTATGCG+AGG + chr4_1:57853970-57853989 Msa0558840:intron 20.0%
! TCATCTACGATATGAATATA+TGG - chr4_1:57853552-57853571 None:intergenic 25.0%
! TAATGGAAGATGTTAATTAG+GGG + chr4_1:57853660-57853679 Msa0558840:intron 25.0%
! TCATAGAAACCAAGATATTA+GGG - chr4_1:57853843-57853862 None:intergenic 25.0%
! ATCATAGAAACCAAGATATT+AGG - chr4_1:57853844-57853863 None:intergenic 25.0%
!! TTTTGTGAATCTTAACTTAC+TGG - chr4_1:57853881-57853900 None:intergenic 25.0%
! TCATCTACGATATGAATATA+TGG - chr4_1:57853552-57853571 None:intergenic 25.0%
! TAATGGAAGATGTTAATTAG+GGG + chr4_1:57853660-57853679 Msa0558840:intron 25.0%
! TCATAGAAACCAAGATATTA+GGG - chr4_1:57853843-57853862 None:intergenic 25.0%
! ATCATAGAAACCAAGATATT+AGG - chr4_1:57853844-57853863 None:intergenic 25.0%
!! TTTTGTGAATCTTAACTTAC+TGG - chr4_1:57853881-57853900 None:intergenic 25.0%
!! AACTCCTGTTTGTTTCTTTT+TGG - chr4_1:57853456-57853475 None:intergenic 30.0%
AAAAAGAAACAAACAGGAGT+TGG + chr4_1:57853455-57853474 Msa0558840:CDS 30.0%
AATGGAAGATGTTAATTAGG+GGG + chr4_1:57853661-57853680 Msa0558840:intron 30.0%
! CATGCTAATGCAAATTTTGA+TGG - chr4_1:57853697-57853716 None:intergenic 30.0%
ATGATCGTTATCTTGTTGAT+TGG + chr4_1:57853937-57853956 Msa0558840:intron 30.0%
!! AACTCCTGTTTGTTTCTTTT+TGG - chr4_1:57853456-57853475 None:intergenic 30.0%
AAAAAGAAACAAACAGGAGT+TGG + chr4_1:57853455-57853474 Msa0558840:CDS 30.0%
AATGGAAGATGTTAATTAGG+GGG + chr4_1:57853661-57853680 Msa0558840:intron 30.0%
! CATGCTAATGCAAATTTTGA+TGG - chr4_1:57853697-57853716 None:intergenic 30.0%
ATGATCGTTATCTTGTTGAT+TGG + chr4_1:57853937-57853956 Msa0558840:intron 30.0%
AGCTGCTGCAGAAAATAAAT+TGG + chr4_1:57853430-57853449 Msa0558840:CDS 35.0%
TTGGCCAAAAAGAAACAAAC+AGG + chr4_1:57853449-57853468 Msa0558840:CDS 35.0%
!!! GAGCTTCTCTTTTTTGAAAG+CGG - chr4_1:57853493-57853512 None:intergenic 35.0%
AAAAGAGAAGCTCAGAGAAA+TGG + chr4_1:57853500-57853519 Msa0558840:CDS 35.0%
ATGGAAGATGTTAATTAGGG+GGG + chr4_1:57853662-57853681 Msa0558840:intron 35.0%
GAGAAACATCACAGAAGATA+AGG - chr4_1:57853816-57853835 None:intergenic 35.0%
TTCTCACTTCCCTAATATCT+TGG + chr4_1:57853831-57853850 Msa0558840:intron 35.0%
ATCTTAACTTACTGGCAGAA+GGG - chr4_1:57853873-57853892 None:intergenic 35.0%
AATCTTAACTTACTGGCAGA+AGG - chr4_1:57853874-57853893 None:intergenic 35.0%
AGCTGCTGCAGAAAATAAAT+TGG + chr4_1:57853430-57853449 Msa0558840:CDS 35.0%
TTGGCCAAAAAGAAACAAAC+AGG + chr4_1:57853449-57853468 Msa0558840:CDS 35.0%
!!! GAGCTTCTCTTTTTTGAAAG+CGG - chr4_1:57853493-57853512 None:intergenic 35.0%
AAAAGAGAAGCTCAGAGAAA+TGG + chr4_1:57853500-57853519 Msa0558840:CDS 35.0%
ATGGAAGATGTTAATTAGGG+GGG + chr4_1:57853662-57853681 Msa0558840:intron 35.0%
GAGAAACATCACAGAAGATA+AGG - chr4_1:57853816-57853835 None:intergenic 35.0%
TTCTCACTTCCCTAATATCT+TGG + chr4_1:57853831-57853850 Msa0558840:intron 35.0%
ATCTTAACTTACTGGCAGAA+GGG - chr4_1:57853873-57853892 None:intergenic 35.0%
AATCTTAACTTACTGGCAGA+AGG - chr4_1:57853874-57853893 None:intergenic 35.0%
! TAGTTTTCTTCCTACCCTTG+TGG - chr4_1:57853370-57853389 None:intergenic 40.0%
AAAACTACTGACGCTGAAAG+TGG + chr4_1:57853383-57853402 Msa0558840:CDS 40.0%
AAACTACTGACGCTGAAAGT+GGG + chr4_1:57853384-57853403 Msa0558840:CDS 40.0%
AAGATGTTAATTAGGGGGGT+TGG + chr4_1:57853666-57853685 Msa0558840:intron 40.0%
AGATGTTAATTAGGGGGGTT+GGG + chr4_1:57853667-57853686 Msa0558840:intron 40.0%
GCTGTGTTAGTCATATAAGC+AGG + chr4_1:57853782-57853801 Msa0558840:intron 40.0%
! TGATTTAAGACAGCGTCTAG+GGG - chr4_1:57854034-57854053 None:intergenic 40.0%
! CTGATTTAAGACAGCGTCTA+GGG - chr4_1:57854035-57854054 None:intergenic 40.0%
! TGTTTTGCTTGCAGAGCTAA+TGG + chr4_1:57854108-57854127 Msa0558840:intron 40.0%
! TAGTTTTCTTCCTACCCTTG+TGG - chr4_1:57853370-57853389 None:intergenic 40.0%
AAAACTACTGACGCTGAAAG+TGG + chr4_1:57853383-57853402 Msa0558840:CDS 40.0%
AAACTACTGACGCTGAAAGT+GGG + chr4_1:57853384-57853403 Msa0558840:CDS 40.0%
AAGATGTTAATTAGGGGGGT+TGG + chr4_1:57853666-57853685 Msa0558840:intron 40.0%
AGATGTTAATTAGGGGGGTT+GGG + chr4_1:57853667-57853686 Msa0558840:intron 40.0%
GCTGTGTTAGTCATATAAGC+AGG + chr4_1:57853782-57853801 Msa0558840:intron 40.0%
! TGATTTAAGACAGCGTCTAG+GGG - chr4_1:57854034-57854053 None:intergenic 40.0%
! CTGATTTAAGACAGCGTCTA+GGG - chr4_1:57854035-57854054 None:intergenic 40.0%
! TGTTTTGCTTGCAGAGCTAA+TGG + chr4_1:57854108-57854127 Msa0558840:intron 40.0%
GATGTTAATTAGGGGGGTTG+GGG + chr4_1:57853668-57853687 Msa0558840:intron 45.0%
ATGTTAATTAGGGGGGTTGG+GGG + chr4_1:57853669-57853688 Msa0558840:intron 45.0%
CGAGGCTCATTCATGCTTTA+CGG + chr4_1:57853988-57854007 Msa0558840:intron 45.0%
! GCTGATTTAAGACAGCGTCT+AGG - chr4_1:57854036-57854055 None:intergenic 45.0%
GGAACTGCAGAGCAAATTTG+AGG + chr4_1:57854129-57854148 Msa0558840:CDS 45.0%
ACTGCAGAGCAAATTTGAGG+AGG + chr4_1:57854132-57854151 Msa0558840:CDS 45.0%
GATGTTAATTAGGGGGGTTG+GGG + chr4_1:57853668-57853687 Msa0558840:intron 45.0%
ATGTTAATTAGGGGGGTTGG+GGG + chr4_1:57853669-57853688 Msa0558840:intron 45.0%
CGAGGCTCATTCATGCTTTA+CGG + chr4_1:57853988-57854007 Msa0558840:intron 45.0%
! GCTGATTTAAGACAGCGTCT+AGG - chr4_1:57854036-57854055 None:intergenic 45.0%
GGAACTGCAGAGCAAATTTG+AGG + chr4_1:57854129-57854148 Msa0558840:CDS 45.0%
ACTGCAGAGCAAATTTGAGG+AGG + chr4_1:57854132-57854151 Msa0558840:CDS 45.0%
!!! ATAAAATCATATAATTTTTA+AGG - chr4_1:57853967-57853986 None:intergenic 5.0%
!!! ATAAAATCATATAATTTTTA+AGG - chr4_1:57853967-57853986 None:intergenic 5.0%
AAAGAAGCCCTTGCTGCAGA+AGG + chr4_1:57853323-57853342 Msa0558840:CDS 50.0%
GCTGAAAGTGGGATTGCTGT+GGG + chr4_1:57853395-57853414 Msa0558840:CDS 50.0%
TCTAGGGGTGAGGGAAAATG+GGG - chr4_1:57854019-57854038 None:intergenic 50.0%
GTCTAGGGGTGAGGGAAAAT+GGG - chr4_1:57854020-57854039 None:intergenic 50.0%
TGCAACAGTTGAGAGCTGCT+AGG + chr4_1:57854167-57854186 Msa0558840:CDS 50.0%
AAAGAAGCCCTTGCTGCAGA+AGG + chr4_1:57853323-57853342 Msa0558840:CDS 50.0%
GCTGAAAGTGGGATTGCTGT+GGG + chr4_1:57853395-57853414 Msa0558840:CDS 50.0%
TCTAGGGGTGAGGGAAAATG+GGG - chr4_1:57854019-57854038 None:intergenic 50.0%
GTCTAGGGGTGAGGGAAAAT+GGG - chr4_1:57854020-57854039 None:intergenic 50.0%
TGCAACAGTTGAGAGCTGCT+AGG + chr4_1:57854167-57854186 Msa0558840:CDS 50.0%
CCATCCTCCTTCTGCAGCAA+GGG - chr4_1:57853333-57853352 None:intergenic 55.0%
TCCATCCTCCTTCTGCAGCA+AGG - chr4_1:57853334-57853353 None:intergenic 55.0%
TGCTGCAGAAGGAGGATGGA+CGG + chr4_1:57853334-57853353 Msa0558840:CDS 55.0%
ACGGTGGTTCAGCACCACAA+GGG + chr4_1:57853353-57853372 Msa0558840:CDS 55.0%
TGGTTCAGCACCACAAGGGT+AGG + chr4_1:57853357-57853376 Msa0558840:CDS 55.0%
CGCTGAAAGTGGGATTGCTG+TGG + chr4_1:57853394-57853413 Msa0558840:CDS 55.0%
AAGTGGGATTGCTGTGGGCT+CGG + chr4_1:57853400-57853419 Msa0558840:CDS 55.0%
CGTCTAGGGGTGAGGGAAAA+TGG - chr4_1:57854021-57854040 None:intergenic 55.0%
AAGACAGCGTCTAGGGGTGA+GGG - chr4_1:57854028-57854047 None:intergenic 55.0%
TAAGACAGCGTCTAGGGGTG+AGG - chr4_1:57854029-57854048 None:intergenic 55.0%
CCATCCTCCTTCTGCAGCAA+GGG - chr4_1:57853333-57853352 None:intergenic 55.0%
TCCATCCTCCTTCTGCAGCA+AGG - chr4_1:57853334-57853353 None:intergenic 55.0%
TGCTGCAGAAGGAGGATGGA+CGG + chr4_1:57853334-57853353 Msa0558840:CDS 55.0%
ACGGTGGTTCAGCACCACAA+GGG + chr4_1:57853353-57853372 Msa0558840:CDS 55.0%
TGGTTCAGCACCACAAGGGT+AGG + chr4_1:57853357-57853376 Msa0558840:CDS 55.0%
CGCTGAAAGTGGGATTGCTG+TGG + chr4_1:57853394-57853413 Msa0558840:CDS 55.0%
AAGTGGGATTGCTGTGGGCT+CGG + chr4_1:57853400-57853419 Msa0558840:CDS 55.0%
CGTCTAGGGGTGAGGGAAAA+TGG - chr4_1:57854021-57854040 None:intergenic 55.0%
AAGACAGCGTCTAGGGGTGA+GGG - chr4_1:57854028-57854047 None:intergenic 55.0%
TAAGACAGCGTCTAGGGGTG+AGG - chr4_1:57854029-57854048 None:intergenic 55.0%
GAAGCCCTTGCTGCAGAAGG+AGG + chr4_1:57853326-57853345 Msa0558840:CDS 60.0%
CCCTTGCTGCAGAAGGAGGA+TGG + chr4_1:57853330-57853349 Msa0558840:CDS 60.0%
TGCAGAAGGAGGATGGACGG+TGG + chr4_1:57853337-57853356 Msa0558840:CDS 60.0%
GACGGTGGTTCAGCACCACA+AGG + chr4_1:57853352-57853371 Msa0558840:CDS 60.0%
GAAGCCCTTGCTGCAGAAGG+AGG + chr4_1:57853326-57853345 Msa0558840:CDS 60.0%
CCCTTGCTGCAGAAGGAGGA+TGG + chr4_1:57853330-57853349 Msa0558840:CDS 60.0%
TGCAGAAGGAGGATGGACGG+TGG + chr4_1:57853337-57853356 Msa0558840:CDS 60.0%
GACGGTGGTTCAGCACCACA+AGG + chr4_1:57853352-57853371 Msa0558840:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 57853305 57854207 57853305 ID=Msa0558840;Name=Msa0558840
chr4_1 mRNA 57853305 57854207 57853305 ID=Msa0558840-mRNA-1;Parent=Msa0558840;Name=Msa0558840-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|100
chr4_1 exon 57853305 57853521 57853305 ID=Msa0558840-mRNA-1:exon:17536;Parent=Msa0558840-mRNA-1
chr4_1 exon 57854122 57854207 57854122 ID=Msa0558840-mRNA-1:exon:17537;Parent=Msa0558840-mRNA-1
chr4_1 CDS 57853305 57853521 57853305 ID=Msa0558840-mRNA-1:cds;Parent=Msa0558840-mRNA-1
chr4_1 CDS 57854122 57854207 57854122 ID=Msa0558840-mRNA-1:cds;Parent=Msa0558840-mRNA-1
Gene Sequence

>Msa0558840

ATGCAGGAAAGAAAAGCAAAAGAAGCCCTTGCTGCAGAAGGAGGATGGACGGTGGTTCAGCACCACAAGGGTAGGAAGAAAACTACTGACGCTGAAAGTGGGATTGCTGTGGGCTCGGTTGCTCAAGCTGCTGCAGAAAATAAATTGGCCAAAAAGAAACAAACAGGAGTTGGTCAAGATTTCTACCGCTTTCAAAAAAGAGAAGCTCAGAGAAATGAGCTAATGGAACTGCAGAGCAAATTTGAGGAGGATAAAAAACGTCTGCAACAGTTGAGAGCTGCTAGGAAATTTAGACCTTATTAA

Protein sequence

>Msa0558840

MQERKAKEALAAEGGWTVVQHHKGRKKTTDAESGIAVGSVAQAAAENKLAKKKQTGVGQDFYRFQKREAQRNELMELQSKFEEDKKRLQQLRAARKFRPY*