AlfalfaGEDB | Msa1256910

M. sativa cultivar zhongmu-4 / Msa1256910

KEGG: K19042
GO: -
PFAM: PF13920.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1256910	XP_024636509.1	90.462	346	14	7	1	341	1	332	0.0	518

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1256910	sp\|Q9FHE4\|BRG1_ARATH	35.610	205	126	3	139	341	93	293	6.05e-29	116

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0037180	Msa1256910	0.824021	1.011359e-53	-8.615850e-47
Msa0037270	Msa1256910	-0.814770	1.300509e-51	-8.615850e-47
Msa0072390	Msa1256910	-0.827405	1.593597e-54	-8.615850e-47
Msa0081120	Msa1256910	-0.831606	1.517937e-55	-8.615850e-47
Msa0092220	Msa1256910	0.823738	1.178527e-53	-8.615850e-47
Msa0105590	Msa1256910	-0.827994	1.150370e-54	-8.615850e-47
Msa0608780	Msa1256910	-0.820645	6.149670e-53	-8.615850e-47
Msa0638300	Msa1256910	-0.811915	5.513041e-51	-8.615850e-47
Msa0649250	Msa1256910	-0.832691	8.180341e-56	-8.615850e-47
Msa0695140	Msa1256910	0.802881	4.545117e-49	-8.615850e-47
Msa0702690	Msa1256910	-0.811003	8.699930e-51	-8.615850e-47
Msa0707880	Msa1256910	-0.830217	3.325246e-55	-8.615850e-47
Msa0721930	Msa1256910	0.800117	1.675461e-48	-8.615850e-47
Msa0732970	Msa1256910	0.809671	1.686343e-50	-8.615850e-47
Msa0733240	Msa1256910	-0.818291	2.116778e-52	-8.615850e-47
Msa0738460	Msa1256910	-0.816198	6.257638e-52	-8.615850e-47
Msa0754020	Msa1256910	-0.805926	1.054513e-49	-8.615850e-47
Msa0762930	Msa1256910	0.804084	2.559859e-49	-8.615850e-47
Msa0769910	Msa1256910	-0.818123	2.309827e-52	-8.615850e-47
Msa0773960	Msa1256910	0.826701	2.347648e-54	-8.615850e-47
Msa0788160	Msa1256910	-0.806205	9.211152e-50	-8.615850e-47
Msa0801920	Msa1256910	-0.806328	8.675855e-50	-8.615850e-47
Msa0802950	Msa1256910	-0.802940	4.418906e-49	-8.615850e-47
Msa0804170	Msa1256910	0.823793	1.143782e-53	-8.615850e-47
Msa0807100	Msa1256910	-0.804773	1.838737e-49	-8.615850e-47
Msa0807870	Msa1256910	-0.804918	1.715395e-49	-8.615850e-47
Msa0823310	Msa1256910	-0.815028	1.140400e-51	-8.615850e-47
Msa0833030	Msa1256910	-0.805276	1.443582e-49	-8.615850e-47
Msa0838120	Msa1256910	-0.803454	3.459157e-49	-8.615850e-47
Msa0842180	Msa1256910	0.817501	3.191042e-52	-8.615850e-47
Msa0132080	Msa1256910	-0.808333	3.260456e-50	-8.615850e-47
Msa0163960	Msa1256910	-0.801297	9.620724e-49	-8.615850e-47
Msa0177320	Msa1256910	0.818515	1.883152e-52	-8.615850e-47
Msa0180100	Msa1256910	-0.801589	8.384894e-49	-8.615850e-47
Msa0206370	Msa1256910	0.806454	8.162002e-50	-8.615850e-47
Msa0211460	Msa1256910	0.824742	6.846635e-54	-8.615850e-47
Msa0220090	Msa1256910	0.823022	1.732229e-53	-8.615850e-47
Msa0225070	Msa1256910	-0.841405	4.846589e-58	-8.615850e-47
Msa0229350	Msa1256910	-0.803186	3.931686e-49	-8.615850e-47
Msa1225140	Msa1256910	-0.805357	1.387810e-49	-8.615850e-47
Msa1225890	Msa1256910	0.819469	1.142381e-52	-8.615850e-47
Msa1228460	Msa1256910	-0.803421	3.514049e-49	-8.615850e-47
Msa1236860	Msa1256910	-0.813053	3.110038e-51	-8.615850e-47
Msa1256910	Msa1273320	0.805292	1.432403e-49	-8.615850e-47
Msa1256910	Msa1281780	0.826391	2.784293e-54	-8.615850e-47
Msa1256910	Msa1286860	-0.825869	3.705048e-54	-8.615850e-47
Msa1256910	Msa1291070	-0.830690	2.548015e-55	-8.615850e-47
Msa1256910	Msa1295210	-0.820687	6.013103e-53	-8.615850e-47
Msa1256910	Msa1297220	-0.841470	4.661456e-58	-8.615850e-47
Msa1256910	Msa1298480	-0.804275	2.336151e-49	-8.615850e-47
Msa1256910	Msa1317240	-0.834801	2.429621e-56	-8.615850e-47
Msa1256910	Msa1323260	-0.868252	7.496379e-66	-8.615850e-47
Msa1256910	Msa1342360	-0.828945	6.780930e-55	-8.615850e-47
Msa1256910	Msa1343450	-0.828245	1.000548e-54	-8.615850e-47
Msa1256910	Msa1350580	-0.811650	6.295319e-51	-8.615850e-47
Msa1256910	Msa1364190	0.810749	9.872737e-51	-8.615850e-47
Msa1256910	Msa1376010	-0.804685	1.918649e-49	-8.615850e-47
Msa1256910	Msa1379270	-0.812045	5.165115e-51	-8.615850e-47
Msa1256910	Msa1407810	0.812433	4.250499e-51	-8.615850e-47
Msa1256910	Msa1408860	-0.806206	9.204855e-50	-8.615850e-47
Msa1256910	Msa1413020	0.819787	9.668545e-53	-8.615850e-47
Msa1256910	Msa1418840	-0.802267	6.084101e-49	-8.615850e-47
Msa1256910	Msa1433360	0.801726	7.857397e-49	-8.615850e-47
Msa1256910	Msa1436660	-0.803383	3.577822e-49	-8.615850e-47
Msa1256910	Msa1437780	0.813668	2.277677e-51	-8.615850e-47
Msa1256910	Msa1452560	-0.814932	1.197397e-51	-8.615850e-47
Msa1256910	Msa1456620	-0.808958	2.397894e-50	-8.615850e-47
Msa1256910	Msa1461660	0.820508	6.610804e-53	-8.615850e-47
Msa0364910	Msa1256910	-0.821655	3.597679e-53	-8.615850e-47
Msa0382020	Msa1256910	-0.816900	4.357256e-52	-8.615850e-47
Msa0386880	Msa1256910	0.815246	1.019695e-51	-8.615850e-47
Msa0388210	Msa1256910	0.807507	4.886783e-50	-8.615850e-47
Msa0403740	Msa1256910	-0.832714	8.075644e-56	-8.615850e-47
Msa0456450	Msa1256910	-0.828035	1.124463e-54	-8.615850e-47
Msa0457070	Msa1256910	-0.819328	1.230319e-52	-8.615850e-47
Msa0471130	Msa1256910	-0.813247	2.819826e-51	-8.615850e-47
Msa1066150	Msa1256910	0.814238	1.705972e-51	-8.615850e-47
Msa1070630	Msa1256910	-0.820151	7.982631e-53	-8.615850e-47
Msa1078600	Msa1256910	-0.802881	4.545117e-49	-8.615850e-47
Msa1078770	Msa1256910	-0.808543	2.940912e-50	-8.615850e-47
Msa1089300	Msa1256910	0.807528	4.836426e-50	-8.615850e-47
Msa1093190	Msa1256910	-0.808933	2.428130e-50	-8.615850e-47
Msa0266140	Msa1256910	-0.852218	5.332999e-61	-8.615850e-47
Msa0267510	Msa1256910	-0.801458	8.919614e-49	-8.615850e-47
Msa0270730	Msa1256910	-0.813194	2.896383e-51	-8.615850e-47
Msa0279810	Msa1256910	0.814843	1.253138e-51	-8.615850e-47
Msa0290800	Msa1256910	0.816659	4.935298e-52	-8.615850e-47
Msa0299030	Msa1256910	0.811111	8.245407e-51	-8.615850e-47
Msa0304060	Msa1256910	-0.845399	4.160738e-59	-8.615850e-47
Msa0308750	Msa1256910	0.803727	3.036711e-49	-8.615850e-47
Msa0316740	Msa1256910	0.803979	2.692251e-49	-8.615850e-47
Msa0319220	Msa1256910	0.816015	6.874868e-52	-8.615850e-47
Msa0328870	Msa1256910	0.841143	5.679936e-58	-8.615850e-47
Msa0354600	Msa1256910	0.806174	9.351495e-50	-8.615850e-47
Msa0364030	Msa1256910	-0.834204	3.431915e-56	-8.615850e-47
Msa1108560	Msa1256910	0.803487	3.406039e-49	-8.615850e-47
Msa1132880	Msa1256910	-0.800298	1.538993e-48	-8.615850e-47
Msa1143860	Msa1256910	-0.805242	1.467088e-49	-8.615850e-47
Msa1158450	Msa1256910	-0.812550	4.007666e-51	-8.615850e-47
Msa1169600	Msa1256910	-0.819429	1.166812e-52	-8.615850e-47
Msa1191530	Msa1256910	-0.806304	8.778943e-50	-8.615850e-47
Msa1205270	Msa1256910	-0.840759	7.164064e-58	-8.615850e-47
Msa1206220	Msa1256910	0.945681	1.935893e-104	-8.615850e-47
Msa1209990	Msa1256910	-0.803916	2.773923e-49	-8.615850e-47
Msa0567330	Msa1256910	0.819277	1.263950e-52	-8.615850e-47
Msa0567570	Msa1256910	0.805331	1.405770e-49	-8.615850e-47
Msa0572730	Msa1256910	-0.804758	1.852599e-49	-8.615850e-47
Msa0576320	Msa1256910	0.816821	4.538751e-52	-8.615850e-47
Msa0576500	Msa1256910	-0.831179	1.932629e-55	-8.615850e-47
Msa0582270	Msa1256910	-0.815390	9.476171e-52	-8.615850e-47
Msa0583390	Msa1256910	0.823536	1.313740e-53	-8.615850e-47
Msa0595530	Msa1256910	0.808246	3.402545e-50	-8.615850e-47
Msa0848670	Msa1256910	-0.825720	4.018657e-54	-8.615850e-47
Msa0852090	Msa1256910	-0.809617	1.732142e-50	-8.615850e-47
Msa0861400	Msa1256910	-0.800281	1.550949e-48	-8.615850e-47
Msa0862230	Msa1256910	-0.817489	3.211638e-52	-8.615850e-47
Msa0868610	Msa1256910	0.800911	1.153906e-48	-8.615850e-47
Msa0873880	Msa1256910	0.805794	1.123929e-49	-8.615850e-47
Msa0898830	Msa1256910	-0.816948	4.249958e-52	-8.615850e-47
Msa0906680	Msa1256910	0.802247	6.141015e-49	-8.615850e-47
Msa0913140	Msa1256910	-0.801980	6.970808e-49	-8.615850e-47
Msa0932140	Msa1256910	0.807882	4.067177e-50	-8.615850e-47
Msa0964400	Msa1256910	-0.809037	2.305922e-50	-8.615850e-47
Msa0967900	Msa1256910	-0.845402	4.153520e-59	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1256910	MtrunA17_Chr4g0055201	90.462	346	14	7	1	341	1	332	0.0	518
Msa1256910	MtrunA17_Chr5g0400041	55.679	361	114	9	1	341	1	335	7.38e-114	333
Msa1256910	MtrunA17_Chr1g0153261	32.510	243	141	4	101	340	118	340	6.10e-32	122
Msa1256910	MtrunA17_Chr4g0043501	32.075	212	136	3	132	341	88	293	1.80e-30	117
Msa1256910	MtrunA17_Chr2g0311981	31.076	251	133	6	128	341	119	366	3.13e-29	115
Msa1256910	MtrunA17_Chr8g0358261	29.858	211	126	3	152	340	99	309	5.27e-26	105
Msa1256910	MtrunA17_Chr4g0018631	28.205	195	128	2	145	339	28	210	2.62e-25	101
Msa1256910	MtrunA17_Chr3g0145641	34.225	187	100	5	154	340	19	182	3.38e-25	100
Msa1256910	MtrunA17_Chr2g0291661	30.348	201	128	3	148	341	124	319	8.55e-24	99.8
Msa1256910	MtrunA17_Chr4g0017931	26.667	240	142	8	112	338	30	248	9.75e-13	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1256910	AT5G47050.1	43.767	361	122	13	1	341	1	300	5.13e-71	223
Msa1256910	AT1G32740.1	41.944	360	140	12	1	340	1	311	6.73e-70	221
Msa1256910	AT4G17680.1	40.678	354	139	10	1	340	18	314	2.97e-69	219
Msa1256910	AT1G32740.4	41.593	339	135	10	21	340	6	300	4.65e-66	211
Msa1256910	AT1G32740.3	42.034	295	119	6	64	340	7	267	3.27e-62	199
Msa1256910	AT1G32740.2	42.034	295	119	6	64	340	7	267	3.27e-62	199
Msa1256910	AT4G17680.2	43.866	269	105	6	86	340	6	242	3.21e-61	196
Msa1256910	AT4G17680.3	47.340	188	79	4	163	340	13	190	3.14e-46	155
Msa1256910	AT1G10650.1	32.000	250	151	5	99	341	102	339	9.84e-40	143
Msa1256910	AT1G10650.2	32.000	250	151	5	99	341	46	283	1.07e-39	142
Msa1256910	AT1G60610.2	29.839	248	157	2	99	340	103	339	7.44e-37	135
Msa1256910	AT1G60610.4	29.839	248	157	2	99	340	103	339	7.44e-37	135
Msa1256910	AT1G60610.1	29.839	248	157	2	99	340	103	339	7.44e-37	135
Msa1256910	AT1G60610.5	29.839	248	157	2	99	340	103	339	7.44e-37	135
Msa1256910	AT1G60610.3	29.839	248	157	2	99	340	103	339	7.44e-37	135
Msa1256910	AT5G45100.2	35.610	205	126	3	139	341	66	266	4.26e-30	115
Msa1256910	AT5G45100.1	35.610	205	126	3	139	341	93	293	6.15e-30	116
Msa1256910	AT1G45976.1	29.167	240	146	3	101	340	110	325	2.42e-29	115
Msa1256910	AT4G19700.1	35.407	209	128	4	136	341	99	303	7.50e-29	113
Msa1256910	AT4G35070.1	33.166	199	119	5	148	340	74	264	3.03e-28	110
Msa1256910	AT4G35070.2	33.166	199	119	5	148	340	19	209	8.67e-28	108
Msa1256910	AT3G12920.1	28.302	212	137	3	143	341	125	334	2.32e-26	107
Msa1256910	AT1G79110.3	29.717	212	129	3	148	341	123	332	7.69e-26	105
Msa1256910	AT1G79110.2	29.717	212	129	3	148	341	145	354	7.91e-26	106
Msa1256910	AT1G79110.1	29.767	215	128	4	148	341	145	357	1.90e-24	102

Find 112 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTATTCTGTTTATTTAATT+TGG	0.130401	8_2:+57192102	Msa1256910:three_prime_UTR
CTTGGGCTTCCATGGTTAAC+TGG	0.184505	8_2:-57191484	None:intergenic
GAGAAGCTGCGGCGGCTTCT+TGG	0.211237	8_2:-57191529	None:intergenic
ACAATAACAATAAATTATTT+AGG	0.246703	8_2:-57191894	None:intergenic
GACTTCAACGGTTGAATTCT+TGG	0.271030	8_2:-57191819	None:intergenic
TACATTACCAAATAAAAGTT+TGG	0.303341	8_2:+57192000	Msa1256910:three_prime_UTR
AGAAGCTGCGGCGGCTTCTT+GGG	0.312290	8_2:-57191528	None:intergenic
AGAAAAGGAAGAAGAGAAAG+AGG	0.314812	8_2:+57190325	Msa1256910:five_prime_UTR
CTGCGGCGGCTTCTTGGGCC+CGG	0.322779	8_2:-57191523	None:intergenic
AAGAGGGAGCGAGCAGAATA+AGG	0.327515	8_2:+57190342	Msa1256910:five_prime_UTR
GTTGTTGTTGTTGAGATTGT+TGG	0.334601	8_2:-57191079	None:intergenic
ACATTATTGTTTGTTGCAGC+TGG	0.354145	8_2:-57191559	None:intergenic
TTTAGAAGAAGAATATTTGA+TGG	0.355647	8_2:-57190517	None:intergenic
TCTCTTCTCTCTATTGTTGA+GGG	0.362553	8_2:-57190459	None:intergenic
AGAGAGAAGAGAAGAATAAC+AGG	0.366849	8_2:+57190470	Msa1256910:five_prime_UTR
GCAACTTCCACCACTCTATC+AGG	0.376811	8_2:-57191668	None:intergenic
CAACAACCATCAGGAGCATT+AGG	0.380479	8_2:+57190714	Msa1256910:CDS
TAAACAACAACAACAATAAC+AGG	0.387069	8_2:+57190536	Msa1256910:CDS
TGCGGCGGCTTCTTGGGCCC+GGG	0.389790	8_2:-57191522	None:intergenic
TTACCTGGGTTTGAAGATAT+TGG	0.390243	8_2:-57191187	None:intergenic
CCACTTCTTTCTCTCTTAAC+CGG	0.391453	8_2:-57191412	None:intergenic
CTGCCATACTTGGGCTTCCA+TGG	0.410747	8_2:-57191492	None:intergenic
TAACCATGGAAGCCCAAGTA+TGG	0.415275	8_2:+57191489	Msa1256910:CDS
GAGAAATGATTAATAACGTT+AGG	0.416596	8_2:-57190933	None:intergenic
GGCCAGTAGAGACAACATTA+TGG	0.416627	8_2:-57190998	None:intergenic
GGCGAAGATGCCGGCGGAGG+CGG	0.418507	8_2:+57191590	Msa1256910:CDS
TTCTTGGGAGTAAAGCAAAC+AGG	0.420487	8_2:-57191803	None:intergenic
GCATGAAACGCCGCCTCCGC+CGG	0.421324	8_2:-57191600	None:intergenic
GTGGAAGTTGCGGCGGCGCG+TGG	0.427360	8_2:+57191680	Msa1256910:CDS
ACTTCAACGGTTGAATTCTT+GGG	0.428768	8_2:-57191818	None:intergenic
GCAGAATAAGGTGTCTCTCT+TGG	0.432226	8_2:+57190354	Msa1256910:five_prime_UTR
TTCTCTTCTCTCTATTGTTG+AGG	0.433027	8_2:-57190460	None:intergenic
AGAAATGATTAATAACGTTA+GGG	0.438438	8_2:-57190932	None:intergenic
CTCCATAATGTTGTCTCTAC+TGG	0.443287	8_2:+57190996	Msa1256910:CDS
GTAGTTGTAAAGAAAAGAAA+AGG	0.445047	8_2:+57190310	Msa1256910:five_prime_UTR
GCCTCCGCCGGCATCTTCGC+CGG	0.446322	8_2:-57191588	None:intergenic
ACAAGCTGAGGATGCTGAAT+CGG	0.449705	8_2:+57191634	Msa1256910:CDS
CGGAGGAAGCGGTGGCGCAC+CGG	0.460350	8_2:+57191393	Msa1256910:CDS
CATTATTGTTTGTTGCAGCT+GGG	0.472699	8_2:-57191558	None:intergenic
AAGTATGGCAGGCAAAAGCC+CGG	0.473882	8_2:+57191504	Msa1256910:CDS
TGAATAATTTGAGAAGGTTG+AGG	0.479716	8_2:-57190963	None:intergenic
ACCGGCGAAGATGCCGGCGG+AGG	0.485312	8_2:+57191587	Msa1256910:CDS
ATTGTTCATATACTAGTCTC+TGG	0.486629	8_2:+57191912	Msa1256910:three_prime_UTR
GGTGTAAGTGTGTGTAAGTA+AGG	0.490620	8_2:+57190255	Msa1256910:five_prime_UTR
TTCTCTCTATTGTTGAGGGA+GGG	0.499938	8_2:-57190455	None:intergenic
CGCGGATTTAGAAGCGCGTA+CGG	0.500807	8_2:+57191460	Msa1256910:CDS
CTCTATTGTTGAGGGAGGGA+GGG	0.501072	8_2:-57190451	None:intergenic
ACTAAACGCGGCGGAGGAAG+CGG	0.504819	8_2:+57191382	Msa1256910:CDS
GGCGTTTCATGCGGCGTGGA+AGG	0.505182	8_2:+57191611	Msa1256910:CDS
TCTCTATTGTTGAGGGAGGG+AGG	0.506421	8_2:-57190452	None:intergenic
GAAAAGGAAGAAGAGAAAGA+GGG	0.507244	8_2:+57190326	Msa1256910:five_prime_UTR
AACAGCAGAAATGGACAAGA+AGG	0.511556	8_2:+57190669	Msa1256910:intron
TCTCAAACCGGCGAAGATGC+CGG	0.511840	8_2:+57191581	Msa1256910:CDS
AGGTAGAATAAGAAGAGGCA+AGG	0.520040	8_2:+57190280	Msa1256910:five_prime_UTR
TTGGGCTTCCATGGTTAACT+GGG	0.521256	8_2:-57191483	None:intergenic
GGCGGAGGCGGCGTTTCATG+CGG	0.524962	8_2:+57191602	Msa1256910:CDS
GAAAGATGAATAATTTGAGA+AGG	0.525108	8_2:-57190969	None:intergenic
GCGGCGCGTGGGAAATGTAG+AGG	0.525683	8_2:+57191692	Msa1256910:CDS
TTGTGTTGTTATTGTTGCAT+AGG	0.525698	8_2:-57190776	None:intergenic
CTTCTCTCTATTGTTGAGGG+AGG	0.525887	8_2:-57190456	None:intergenic
GAATCTACGGCGGACATTGG+CGG	0.530434	8_2:+57191331	Msa1256910:CDS
GTGTGAGAAGCGCGTGGCAA+CGG	0.531560	8_2:+57191715	Msa1256910:CDS
AAGTGTGTGTAAGTAAGGTA+AGG	0.536383	8_2:+57190260	Msa1256910:five_prime_UTR
CATGGAAGCCCAAGTATGGC+AGG	0.538619	8_2:+57191493	Msa1256910:CDS
GGCGGACATTGGCGGAGAAG+AGG	0.541874	8_2:+57191339	Msa1256910:CDS
TCTCGCAAGAGAGGAAGAGA+AGG	0.542174	8_2:+57190903	Msa1256910:CDS
GGTAGAATAAGAAGAGGCAA+GGG	0.543321	8_2:+57190281	Msa1256910:five_prime_UTR
CAAACAATAATGTCTCAAAC+CGG	0.545553	8_2:+57191569	Msa1256910:CDS
TTACAACAACAACAACCATC+AGG	0.547918	8_2:+57190705	Msa1256910:CDS
AGAGAATCTACGGCGGACAT+TGG	0.549805	8_2:+57191328	Msa1256910:CDS
TGGAAGTTGCGGCGGCGCGT+GGG	0.555769	8_2:+57191681	Msa1256910:CDS
TAAATCCGCGTTCCTGCGAG+TGG	0.563827	8_2:-57191447	None:intergenic
TGATAGAGTGGTGGAAGTTG+CGG	0.565112	8_2:+57191670	Msa1256910:CDS
TGAGAAGCGCGTGGCAACGG+TGG	0.570009	8_2:+57191718	Msa1256910:CDS
AGGTAAGGTAGAATAAGAAG+AGG	0.572929	8_2:+57190275	Msa1256910:five_prime_UTR
TATTTCAGGGAGAGAATCTA+CGG	0.574428	8_2:+57191318	Msa1256910:intron
GGAGCTACTAAACGCGGCGG+AGG	0.576161	8_2:+57191376	Msa1256910:CDS
CCGCCGCGTTTAGTAGCTCC+CGG	0.576958	8_2:-57191373	None:intergenic
CTGGGCTTGTAGAGAAGCTG+CGG	0.587298	8_2:-57191540	None:intergenic
AATTCGCAAAAGCCACTCGC+AGG	0.588745	8_2:+57191435	Msa1256910:CDS
CGGCGCGTGGGAAATGTAGA+GGG	0.594790	8_2:+57191693	Msa1256910:CDS
CAAACACACAAATGCCGACA+AGG	0.601757	8_2:-57191749	None:intergenic
GGCTTGTAGAGAAGCTGCGG+CGG	0.603377	8_2:-57191537	None:intergenic
AGTATGGCAGGCAAAAGCCC+GGG	0.608050	8_2:+57191505	Msa1256910:CDS
AAAAGCCACTCGCAGGAACG+CGG	0.614760	8_2:+57191442	Msa1256910:CDS
AAACGCGGCGGAGGAAGCGG+TGG	0.615069	8_2:+57191385	Msa1256910:CDS
GGTACAAATTCTCGCAAGAG+AGG	0.618293	8_2:+57190894	Msa1256910:intron
AGGCGGCGTTTCATGCGGCG+TGG	0.622888	8_2:+57191607	Msa1256910:CDS
TTATATTGATCCTGATAGAG+TGG	0.624363	8_2:+57191658	Msa1256910:CDS
GATAGTCCTAATGCTCCTGA+TGG	0.626296	8_2:-57190720	None:intergenic
CATGTACATTCATTAACCAA+AGG	0.627810	8_2:-57192045	None:intergenic
ATTTGAGAAACAATTCCTTG+TGG	0.628054	8_2:-57191140	None:intergenic
CTAAGAGAGAAAGACTTCAA+CGG	0.634503	8_2:-57191831	None:intergenic
CGGCGTGGAAGGACAAGCTG+AGG	0.634766	8_2:+57191622	Msa1256910:CDS
TTCTCATCTCTATTACCACA+AGG	0.635335	8_2:+57191125	Msa1256910:CDS
GATCATCGAATGATAAGCGA+AGG	0.636198	8_2:-57191019	None:intergenic
GAAATGATTAATAACGTTAG+GGG	0.644550	8_2:-57190931	None:intergenic
ACAGCAGAAATGGACAAGAA+GGG	0.646393	8_2:+57190670	Msa1256910:intron
ATGACCACATGGTTAACAAG+TGG	0.650500	8_2:+57192065	Msa1256910:three_prime_UTR
TAGAGTGGTGGAAGTTGCGG+CGG	0.650672	8_2:+57191673	Msa1256910:CDS
TATTGATCCTGATAGAGTGG+TGG	0.652755	8_2:+57191661	Msa1256910:CDS
CCGGTTAAGAGAGAAAGAAG+TGG	0.657511	8_2:+57191412	Msa1256910:CDS
TCTTGGGAGTAAAGCAAACA+GGG	0.657653	8_2:-57191802	None:intergenic
AGACCAATATCTTCAAACCC+AGG	0.667723	8_2:+57191184	Msa1256910:CDS
AATGAATGTACATGACCACA+TGG	0.669572	8_2:+57192054	Msa1256910:three_prime_UTR
TAGAGGGTGTGAGAAGCGCG+TGG	0.693712	8_2:+57191709	Msa1256910:CDS
GCGTACGGCCCAGTTAACCA+TGG	0.703157	8_2:+57191475	Msa1256910:CDS
CAAACCGGCGAAGATGCCGG+CGG	0.709631	8_2:+57191584	Msa1256910:CDS
AATGCCACTTGTTAACCATG+TGG	0.717135	8_2:-57192069	None:intergenic
TTACCGGGAGCTACTAAACG+CGG	0.757971	8_2:+57191370	Msa1256910:CDS
TTCAGGGAGAGAATCTACGG+CGG	0.769377	8_2:+57191321	Msa1256910:intron
CCGGGAGCTACTAAACGCGG+CGG	0.776946	8_2:+57191373	Msa1256910:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAATAACAATAAATTATTT+AGG	-	chr8_2:57191897-57191916	None:intergenic	10.0%
!!	TTTATTCTGTTTATTTAATT+TGG	+	chr8_2:57192102-57192121	Msa1256910:three_prime_UTR	10.0%
!!	ACAATAACAATAAATTATTT+AGG	-	chr8_2:57191897-57191916	None:intergenic	10.0%
!!	TTTATTCTGTTTATTTAATT+TGG	+	chr8_2:57192102-57192121	Msa1256910:three_prime_UTR	10.0%
!!!	ATTTTCGATTTATTAGAAAT+TGG	+	chr8_2:57191257-57191276	Msa1256910:intron	15.0%
!!!	AATTTGGTTATTTATTTCTT+TGG	+	chr8_2:57192118-57192137	Msa1256910:three_prime_UTR	15.0%
!!!	ATTTTCGATTTATTAGAAAT+TGG	+	chr8_2:57191257-57191276	Msa1256910:intron	15.0%
!!	TTTAGAAGAAGAATATTTGA+TGG	-	chr8_2:57190520-57190539	None:intergenic	20.0%
!!	TCATATAGAATTGTATTTGA+GGG	-	chr8_2:57190606-57190625	None:intergenic	20.0%
!!	AGAAATGATTAATAACGTTA+GGG	-	chr8_2:57190935-57190954	None:intergenic	20.0%
!!	TTATTAGAAATTGGATAGAA+AGG	+	chr8_2:57191266-57191285	Msa1256910:intron	20.0%
!!	TACATTACCAAATAAAAGTT+TGG	+	chr8_2:57192000-57192019	Msa1256910:three_prime_UTR	20.0%
!!!	ACTAAAACCAAACTTTTATT+TGG	-	chr8_2:57192010-57192029	None:intergenic	20.0%
!!!	TAGATTTTCATTTTTACCTT+TGG	+	chr8_2:57192029-57192048	Msa1256910:three_prime_UTR	20.0%
!!	TTTAGAAGAAGAATATTTGA+TGG	-	chr8_2:57190520-57190539	None:intergenic	20.0%
!!	TCATATAGAATTGTATTTGA+GGG	-	chr8_2:57190606-57190625	None:intergenic	20.0%
!!	AGAAATGATTAATAACGTTA+GGG	-	chr8_2:57190935-57190954	None:intergenic	20.0%
!!	TTATTAGAAATTGGATAGAA+AGG	+	chr8_2:57191266-57191285	Msa1256910:intron	20.0%
!!	TACATTACCAAATAAAAGTT+TGG	+	chr8_2:57192000-57192019	Msa1256910:three_prime_UTR	20.0%
!!!	ACTAAAACCAAACTTTTATT+TGG	-	chr8_2:57192010-57192029	None:intergenic	20.0%
!!!	TAGATTTTCATTTTTACCTT+TGG	+	chr8_2:57192029-57192048	Msa1256910:three_prime_UTR	20.0%
!	GTAGTTGTAAAGAAAAGAAA+AGG	+	chr8_2:57190310-57190329	Msa1256910:five_prime_UTR	25.0%
!	TAAACAACAACAACAATAAC+AGG	+	chr8_2:57190536-57190555	Msa1256910:CDS	25.0%
!	GTCATATAGAATTGTATTTG+AGG	-	chr8_2:57190607-57190626	None:intergenic	25.0%
!	GAAATGATTAATAACGTTAG+GGG	-	chr8_2:57190934-57190953	None:intergenic	25.0%
!	GAGAAATGATTAATAACGTT+AGG	-	chr8_2:57190936-57190955	None:intergenic	25.0%
!	GAAAGATGAATAATTTGAGA+AGG	-	chr8_2:57190972-57190991	None:intergenic	25.0%
!!!	TTGTTTTGTTTTGTTTACCT+GGG	-	chr8_2:57191204-57191223	None:intergenic	25.0%
!!!	TTTGTTTTGTTTTGTTTACC+TGG	-	chr8_2:57191205-57191224	None:intergenic	25.0%
!!	TTTTGAATTTTTATGCGTGA+TGG	+	chr8_2:57191294-57191313	Msa1256910:intron	25.0%
!!	TTTGAATTTTTATGCGTGAT+GGG	+	chr8_2:57191295-57191314	Msa1256910:intron	25.0%
!	GTAGTTGTAAAGAAAAGAAA+AGG	+	chr8_2:57190310-57190329	Msa1256910:five_prime_UTR	25.0%
!	TAAACAACAACAACAATAAC+AGG	+	chr8_2:57190536-57190555	Msa1256910:CDS	25.0%
!	GTCATATAGAATTGTATTTG+AGG	-	chr8_2:57190607-57190626	None:intergenic	25.0%
!	GAAATGATTAATAACGTTAG+GGG	-	chr8_2:57190934-57190953	None:intergenic	25.0%
!	GAGAAATGATTAATAACGTT+AGG	-	chr8_2:57190936-57190955	None:intergenic	25.0%
!	GAAAGATGAATAATTTGAGA+AGG	-	chr8_2:57190972-57190991	None:intergenic	25.0%
!!!	TTGTTTTGTTTTGTTTACCT+GGG	-	chr8_2:57191204-57191223	None:intergenic	25.0%
!!!	TTTGTTTTGTTTTGTTTACC+TGG	-	chr8_2:57191205-57191224	None:intergenic	25.0%
!!	TTTTGAATTTTTATGCGTGA+TGG	+	chr8_2:57191294-57191313	Msa1256910:intron	25.0%
!!	TTTGAATTTTTATGCGTGAT+GGG	+	chr8_2:57191295-57191314	Msa1256910:intron	25.0%
	GAACGAGAAAAGAGATTAAT+TGG	-	chr8_2:57190642-57190661	None:intergenic	30.0%
!	TTGTGTTGTTATTGTTGCAT+AGG	-	chr8_2:57190779-57190798	None:intergenic	30.0%
	TGAATAATTTGAGAAGGTTG+AGG	-	chr8_2:57190966-57190985	None:intergenic	30.0%
	ATTTGAGAAACAATTCCTTG+TGG	-	chr8_2:57191143-57191162	None:intergenic	30.0%
	CAAACAATAATGTCTCAAAC+CGG	+	chr8_2:57191569-57191588	Msa1256910:CDS	30.0%
	TTATATTGATCCTGATAGAG+TGG	+	chr8_2:57191658-57191677	Msa1256910:CDS	30.0%
!	ATTGTTCATATACTAGTCTC+TGG	+	chr8_2:57191912-57191931	Msa1256910:three_prime_UTR	30.0%
	CATGTACATTCATTAACCAA+AGG	-	chr8_2:57192048-57192067	None:intergenic	30.0%
	GAACGAGAAAAGAGATTAAT+TGG	-	chr8_2:57190642-57190661	None:intergenic	30.0%
!	TTGTGTTGTTATTGTTGCAT+AGG	-	chr8_2:57190779-57190798	None:intergenic	30.0%
	TGAATAATTTGAGAAGGTTG+AGG	-	chr8_2:57190966-57190985	None:intergenic	30.0%
	ATTTGAGAAACAATTCCTTG+TGG	-	chr8_2:57191143-57191162	None:intergenic	30.0%
	CAAACAATAATGTCTCAAAC+CGG	+	chr8_2:57191569-57191588	Msa1256910:CDS	30.0%
	TTATATTGATCCTGATAGAG+TGG	+	chr8_2:57191658-57191677	Msa1256910:CDS	30.0%
!	ATTGTTCATATACTAGTCTC+TGG	+	chr8_2:57191912-57191931	Msa1256910:three_prime_UTR	30.0%
	CATGTACATTCATTAACCAA+AGG	-	chr8_2:57192048-57192067	None:intergenic	30.0%
	AAGTGTGTGTAAGTAAGGTA+AGG	+	chr8_2:57190260-57190279	Msa1256910:five_prime_UTR	35.0%
	AGGTAAGGTAGAATAAGAAG+AGG	+	chr8_2:57190275-57190294	Msa1256910:five_prime_UTR	35.0%
	AGAAAAGGAAGAAGAGAAAG+AGG	+	chr8_2:57190325-57190344	Msa1256910:five_prime_UTR	35.0%
	GAAAAGGAAGAAGAGAAAGA+GGG	+	chr8_2:57190326-57190345	Msa1256910:five_prime_UTR	35.0%
	TCTCTTCTCTCTATTGTTGA+GGG	-	chr8_2:57190462-57190481	None:intergenic	35.0%
	TTCTCTTCTCTCTATTGTTG+AGG	-	chr8_2:57190463-57190482	None:intergenic	35.0%
	AGAGAGAAGAGAAGAATAAC+AGG	+	chr8_2:57190470-57190489	Msa1256910:five_prime_UTR	35.0%
!	TCTTTTCCTAACAGCAGAAA+TGG	+	chr8_2:57190660-57190679	Msa1256910:intron	35.0%
	TTACAACAACAACAACCATC+AGG	+	chr8_2:57190705-57190724	Msa1256910:CDS	35.0%
!	GTTGTTGTTGTTGAGATTGT+TGG	-	chr8_2:57191082-57191101	None:intergenic	35.0%
	TCTCAACAACAACAACAACA+AGG	+	chr8_2:57191086-57191105	Msa1256910:CDS	35.0%
	TTCTCATCTCTATTACCACA+AGG	+	chr8_2:57191125-57191144	Msa1256910:CDS	35.0%
	TTACCTGGGTTTGAAGATAT+TGG	-	chr8_2:57191190-57191209	None:intergenic	35.0%
	TATTTCAGGGAGAGAATCTA+CGG	+	chr8_2:57191318-57191337	Msa1256910:intron	35.0%
	AGAAGAGGCAAAAACATTAC+CGG	+	chr8_2:57191354-57191373	Msa1256910:CDS	35.0%
!	CATTATTGTTTGTTGCAGCT+GGG	-	chr8_2:57191561-57191580	None:intergenic	35.0%
!	ACATTATTGTTTGTTGCAGC+TGG	-	chr8_2:57191562-57191581	None:intergenic	35.0%
	ACTTCAACGGTTGAATTCTT+GGG	-	chr8_2:57191821-57191840	None:intergenic	35.0%
	CTAAGAGAGAAAGACTTCAA+CGG	-	chr8_2:57191834-57191853	None:intergenic	35.0%
	AATGAATGTACATGACCACA+TGG	+	chr8_2:57192054-57192073	Msa1256910:three_prime_UTR	35.0%
	AAGTGTGTGTAAGTAAGGTA+AGG	+	chr8_2:57190260-57190279	Msa1256910:five_prime_UTR	35.0%
	AGGTAAGGTAGAATAAGAAG+AGG	+	chr8_2:57190275-57190294	Msa1256910:five_prime_UTR	35.0%
	AGAAAAGGAAGAAGAGAAAG+AGG	+	chr8_2:57190325-57190344	Msa1256910:five_prime_UTR	35.0%
	GAAAAGGAAGAAGAGAAAGA+GGG	+	chr8_2:57190326-57190345	Msa1256910:five_prime_UTR	35.0%
	TCTCTTCTCTCTATTGTTGA+GGG	-	chr8_2:57190462-57190481	None:intergenic	35.0%
	TTCTCTTCTCTCTATTGTTG+AGG	-	chr8_2:57190463-57190482	None:intergenic	35.0%
	AGAGAGAAGAGAAGAATAAC+AGG	+	chr8_2:57190470-57190489	Msa1256910:five_prime_UTR	35.0%
!	TCTTTTCCTAACAGCAGAAA+TGG	+	chr8_2:57190660-57190679	Msa1256910:intron	35.0%
	TTACAACAACAACAACCATC+AGG	+	chr8_2:57190705-57190724	Msa1256910:CDS	35.0%
!	GTTGTTGTTGTTGAGATTGT+TGG	-	chr8_2:57191082-57191101	None:intergenic	35.0%
	TCTCAACAACAACAACAACA+AGG	+	chr8_2:57191086-57191105	Msa1256910:CDS	35.0%
	TTCTCATCTCTATTACCACA+AGG	+	chr8_2:57191125-57191144	Msa1256910:CDS	35.0%
	TTACCTGGGTTTGAAGATAT+TGG	-	chr8_2:57191190-57191209	None:intergenic	35.0%
	TATTTCAGGGAGAGAATCTA+CGG	+	chr8_2:57191318-57191337	Msa1256910:intron	35.0%
	AGAAGAGGCAAAAACATTAC+CGG	+	chr8_2:57191354-57191373	Msa1256910:CDS	35.0%
!	CATTATTGTTTGTTGCAGCT+GGG	-	chr8_2:57191561-57191580	None:intergenic	35.0%
!	ACATTATTGTTTGTTGCAGC+TGG	-	chr8_2:57191562-57191581	None:intergenic	35.0%
	ACTTCAACGGTTGAATTCTT+GGG	-	chr8_2:57191821-57191840	None:intergenic	35.0%
	CTAAGAGAGAAAGACTTCAA+CGG	-	chr8_2:57191834-57191853	None:intergenic	35.0%
	AATGAATGTACATGACCACA+TGG	+	chr8_2:57192054-57192073	Msa1256910:three_prime_UTR	35.0%
	GGTGTAAGTGTGTGTAAGTA+AGG	+	chr8_2:57190255-57190274	Msa1256910:five_prime_UTR	40.0%
	AGGTAGAATAAGAAGAGGCA+AGG	+	chr8_2:57190280-57190299	Msa1256910:five_prime_UTR	40.0%
	GGTAGAATAAGAAGAGGCAA+GGG	+	chr8_2:57190281-57190300	Msa1256910:five_prime_UTR	40.0%
	TTCTCTCTATTGTTGAGGGA+GGG	-	chr8_2:57190458-57190477	None:intergenic	40.0%
	TCTTGTCCATTTCTGCTGTT+AGG	-	chr8_2:57190669-57190688	None:intergenic	40.0%
	AACAGCAGAAATGGACAAGA+AGG	+	chr8_2:57190669-57190688	Msa1256910:intron	40.0%
	ACAGCAGAAATGGACAAGAA+GGG	+	chr8_2:57190670-57190689	Msa1256910:intron	40.0%
	CTCCATAATGTTGTCTCTAC+TGG	+	chr8_2:57190996-57191015	Msa1256910:CDS	40.0%
	GATCATCGAATGATAAGCGA+AGG	-	chr8_2:57191022-57191041	None:intergenic	40.0%
	AGACCAATATCTTCAAACCC+AGG	+	chr8_2:57191184-57191203	Msa1256910:CDS	40.0%
!!	TTATGCGTGATGGGTATTTC+AGG	+	chr8_2:57191304-57191323	Msa1256910:intron	40.0%
!!	TATGCGTGATGGGTATTTCA+GGG	+	chr8_2:57191305-57191324	Msa1256910:intron	40.0%
	GAAGAGGCAAAAACATTACC+GGG	+	chr8_2:57191355-57191374	Msa1256910:CDS	40.0%
	CCACTTCTTTCTCTCTTAAC+CGG	-	chr8_2:57191415-57191434	None:intergenic	40.0%
	TATTGATCCTGATAGAGTGG+TGG	+	chr8_2:57191661-57191680	Msa1256910:CDS	40.0%
	TCTTGGGAGTAAAGCAAACA+GGG	-	chr8_2:57191805-57191824	None:intergenic	40.0%
	TTCTTGGGAGTAAAGCAAAC+AGG	-	chr8_2:57191806-57191825	None:intergenic	40.0%
	GACTTCAACGGTTGAATTCT+TGG	-	chr8_2:57191822-57191841	None:intergenic	40.0%
	ATGACCACATGGTTAACAAG+TGG	+	chr8_2:57192065-57192084	Msa1256910:three_prime_UTR	40.0%
!	AATGCCACTTGTTAACCATG+TGG	-	chr8_2:57192072-57192091	None:intergenic	40.0%
	GGTGTAAGTGTGTGTAAGTA+AGG	+	chr8_2:57190255-57190274	Msa1256910:five_prime_UTR	40.0%
	AGGTAGAATAAGAAGAGGCA+AGG	+	chr8_2:57190280-57190299	Msa1256910:five_prime_UTR	40.0%
	GGTAGAATAAGAAGAGGCAA+GGG	+	chr8_2:57190281-57190300	Msa1256910:five_prime_UTR	40.0%
	TTCTCTCTATTGTTGAGGGA+GGG	-	chr8_2:57190458-57190477	None:intergenic	40.0%
	TCTTGTCCATTTCTGCTGTT+AGG	-	chr8_2:57190669-57190688	None:intergenic	40.0%
	AACAGCAGAAATGGACAAGA+AGG	+	chr8_2:57190669-57190688	Msa1256910:intron	40.0%
	ACAGCAGAAATGGACAAGAA+GGG	+	chr8_2:57190670-57190689	Msa1256910:intron	40.0%
	CTCCATAATGTTGTCTCTAC+TGG	+	chr8_2:57190996-57191015	Msa1256910:CDS	40.0%
	GATCATCGAATGATAAGCGA+AGG	-	chr8_2:57191022-57191041	None:intergenic	40.0%
	AGACCAATATCTTCAAACCC+AGG	+	chr8_2:57191184-57191203	Msa1256910:CDS	40.0%
!!	TTATGCGTGATGGGTATTTC+AGG	+	chr8_2:57191304-57191323	Msa1256910:intron	40.0%
!!	TATGCGTGATGGGTATTTCA+GGG	+	chr8_2:57191305-57191324	Msa1256910:intron	40.0%
	GAAGAGGCAAAAACATTACC+GGG	+	chr8_2:57191355-57191374	Msa1256910:CDS	40.0%
	CCACTTCTTTCTCTCTTAAC+CGG	-	chr8_2:57191415-57191434	None:intergenic	40.0%
	TATTGATCCTGATAGAGTGG+TGG	+	chr8_2:57191661-57191680	Msa1256910:CDS	40.0%
	TCTTGGGAGTAAAGCAAACA+GGG	-	chr8_2:57191805-57191824	None:intergenic	40.0%
	TTCTTGGGAGTAAAGCAAAC+AGG	-	chr8_2:57191806-57191825	None:intergenic	40.0%
	GACTTCAACGGTTGAATTCT+TGG	-	chr8_2:57191822-57191841	None:intergenic	40.0%
	ATGACCACATGGTTAACAAG+TGG	+	chr8_2:57192065-57192084	Msa1256910:three_prime_UTR	40.0%
!	AATGCCACTTGTTAACCATG+TGG	-	chr8_2:57192072-57192091	None:intergenic	40.0%
!	GCAGAATAAGGTGTCTCTCT+TGG	+	chr8_2:57190354-57190373	Msa1256910:five_prime_UTR	45.0%
	CTTCTCTCTATTGTTGAGGG+AGG	-	chr8_2:57190459-57190478	None:intergenic	45.0%
!!	CAACAACCATCAGGAGCATT+AGG	+	chr8_2:57190714-57190733	Msa1256910:CDS	45.0%
	GATAGTCCTAATGCTCCTGA+TGG	-	chr8_2:57190723-57190742	None:intergenic	45.0%
	ATGTCTCAACAATTCACCGC+AGG	+	chr8_2:57190873-57190892	Msa1256910:intron	45.0%
	CTTGCGAGAATTTGTACCTG+CGG	-	chr8_2:57190892-57190911	None:intergenic	45.0%
	GGTACAAATTCTCGCAAGAG+AGG	+	chr8_2:57190894-57190913	Msa1256910:intron	45.0%
	GGCCAGTAGAGACAACATTA+TGG	-	chr8_2:57191001-57191020	None:intergenic	45.0%
	CCGGTTAAGAGAGAAAGAAG+TGG	+	chr8_2:57191412-57191431	Msa1256910:CDS	45.0%
	TTGGGCTTCCATGGTTAACT+GGG	-	chr8_2:57191486-57191505	None:intergenic	45.0%
	TAACCATGGAAGCCCAAGTA+TGG	+	chr8_2:57191489-57191508	Msa1256910:CDS	45.0%
	ACAAGCTGAGGATGCTGAAT+CGG	+	chr8_2:57191634-57191653	Msa1256910:CDS	45.0%
!	TGATAGAGTGGTGGAAGTTG+CGG	+	chr8_2:57191670-57191689	Msa1256910:CDS	45.0%
!!	CGGTGGTTGTTTTACCTTGT+CGG	+	chr8_2:57191735-57191754	Msa1256910:CDS	45.0%
	CAAACACACAAATGCCGACA+AGG	-	chr8_2:57191752-57191771	None:intergenic	45.0%
!	GCAGAATAAGGTGTCTCTCT+TGG	+	chr8_2:57190354-57190373	Msa1256910:five_prime_UTR	45.0%
	CTTCTCTCTATTGTTGAGGG+AGG	-	chr8_2:57190459-57190478	None:intergenic	45.0%
!!	CAACAACCATCAGGAGCATT+AGG	+	chr8_2:57190714-57190733	Msa1256910:CDS	45.0%
	GATAGTCCTAATGCTCCTGA+TGG	-	chr8_2:57190723-57190742	None:intergenic	45.0%
	ATGTCTCAACAATTCACCGC+AGG	+	chr8_2:57190873-57190892	Msa1256910:intron	45.0%
	CTTGCGAGAATTTGTACCTG+CGG	-	chr8_2:57190892-57190911	None:intergenic	45.0%
	GGTACAAATTCTCGCAAGAG+AGG	+	chr8_2:57190894-57190913	Msa1256910:intron	45.0%
	GGCCAGTAGAGACAACATTA+TGG	-	chr8_2:57191001-57191020	None:intergenic	45.0%
	CCGGTTAAGAGAGAAAGAAG+TGG	+	chr8_2:57191412-57191431	Msa1256910:CDS	45.0%
	TTGGGCTTCCATGGTTAACT+GGG	-	chr8_2:57191486-57191505	None:intergenic	45.0%
	TAACCATGGAAGCCCAAGTA+TGG	+	chr8_2:57191489-57191508	Msa1256910:CDS	45.0%
	ACAAGCTGAGGATGCTGAAT+CGG	+	chr8_2:57191634-57191653	Msa1256910:CDS	45.0%
!	TGATAGAGTGGTGGAAGTTG+CGG	+	chr8_2:57191670-57191689	Msa1256910:CDS	45.0%
!!	CGGTGGTTGTTTTACCTTGT+CGG	+	chr8_2:57191735-57191754	Msa1256910:CDS	45.0%
	CAAACACACAAATGCCGACA+AGG	-	chr8_2:57191752-57191771	None:intergenic	45.0%
	AAGAGGGAGCGAGCAGAATA+AGG	+	chr8_2:57190342-57190361	Msa1256910:five_prime_UTR	50.0%
	CTCTATTGTTGAGGGAGGGA+GGG	-	chr8_2:57190454-57190473	None:intergenic	50.0%
	TCTCTATTGTTGAGGGAGGG+AGG	-	chr8_2:57190455-57190474	None:intergenic	50.0%
	TCTCGCAAGAGAGGAAGAGA+AGG	+	chr8_2:57190903-57190922	Msa1256910:CDS	50.0%
	TTCAGGGAGAGAATCTACGG+CGG	+	chr8_2:57191321-57191340	Msa1256910:intron	50.0%
	AGAGAATCTACGGCGGACAT+TGG	+	chr8_2:57191328-57191347	Msa1256910:CDS	50.0%
	TTACCGGGAGCTACTAAACG+CGG	+	chr8_2:57191370-57191389	Msa1256910:CDS	50.0%
	AATTCGCAAAAGCCACTCGC+AGG	+	chr8_2:57191435-57191454	Msa1256910:CDS	50.0%
	CTTGGGCTTCCATGGTTAAC+TGG	-	chr8_2:57191487-57191506	None:intergenic	50.0%
!	GGCTTTTGCCTGCCATACTT+GGG	-	chr8_2:57191504-57191523	None:intergenic	50.0%
	AAGTATGGCAGGCAAAAGCC+CGG	+	chr8_2:57191504-57191523	Msa1256910:CDS	50.0%
	GCAACTTCCACCACTCTATC+AGG	-	chr8_2:57191671-57191690	None:intergenic	50.0%
	AAGAGGGAGCGAGCAGAATA+AGG	+	chr8_2:57190342-57190361	Msa1256910:five_prime_UTR	50.0%
	CTCTATTGTTGAGGGAGGGA+GGG	-	chr8_2:57190454-57190473	None:intergenic	50.0%
	TCTCTATTGTTGAGGGAGGG+AGG	-	chr8_2:57190455-57190474	None:intergenic	50.0%
	TCTCGCAAGAGAGGAAGAGA+AGG	+	chr8_2:57190903-57190922	Msa1256910:CDS	50.0%
	TTCAGGGAGAGAATCTACGG+CGG	+	chr8_2:57191321-57191340	Msa1256910:intron	50.0%
	AGAGAATCTACGGCGGACAT+TGG	+	chr8_2:57191328-57191347	Msa1256910:CDS	50.0%
	TTACCGGGAGCTACTAAACG+CGG	+	chr8_2:57191370-57191389	Msa1256910:CDS	50.0%
	AATTCGCAAAAGCCACTCGC+AGG	+	chr8_2:57191435-57191454	Msa1256910:CDS	50.0%
	CTTGGGCTTCCATGGTTAAC+TGG	-	chr8_2:57191487-57191506	None:intergenic	50.0%
!	GGCTTTTGCCTGCCATACTT+GGG	-	chr8_2:57191504-57191523	None:intergenic	50.0%
	AAGTATGGCAGGCAAAAGCC+CGG	+	chr8_2:57191504-57191523	Msa1256910:CDS	50.0%
	GCAACTTCCACCACTCTATC+AGG	-	chr8_2:57191671-57191690	None:intergenic	50.0%
	GAATCTACGGCGGACATTGG+CGG	+	chr8_2:57191331-57191350	Msa1256910:CDS	55.0%
	AAAAGCCACTCGCAGGAACG+CGG	+	chr8_2:57191442-57191461	Msa1256910:CDS	55.0%
	TAAATCCGCGTTCCTGCGAG+TGG	-	chr8_2:57191450-57191469	None:intergenic	55.0%
!	CGCGGATTTAGAAGCGCGTA+CGG	+	chr8_2:57191460-57191479	Msa1256910:CDS	55.0%
	CTGCCATACTTGGGCTTCCA+TGG	-	chr8_2:57191495-57191514	None:intergenic	55.0%
	CATGGAAGCCCAAGTATGGC+AGG	+	chr8_2:57191493-57191512	Msa1256910:CDS	55.0%
!	GGGCTTTTGCCTGCCATACT+TGG	-	chr8_2:57191505-57191524	None:intergenic	55.0%
	AGTATGGCAGGCAAAAGCCC+GGG	+	chr8_2:57191505-57191524	Msa1256910:CDS	55.0%
	CTGGGCTTGTAGAGAAGCTG+CGG	-	chr8_2:57191543-57191562	None:intergenic	55.0%
	TCTCAAACCGGCGAAGATGC+CGG	+	chr8_2:57191581-57191600	Msa1256910:CDS	55.0%
!	TAGAGTGGTGGAAGTTGCGG+CGG	+	chr8_2:57191673-57191692	Msa1256910:CDS	55.0%
	GAATCTACGGCGGACATTGG+CGG	+	chr8_2:57191331-57191350	Msa1256910:CDS	55.0%
	AAAAGCCACTCGCAGGAACG+CGG	+	chr8_2:57191442-57191461	Msa1256910:CDS	55.0%
	TAAATCCGCGTTCCTGCGAG+TGG	-	chr8_2:57191450-57191469	None:intergenic	55.0%
!	CGCGGATTTAGAAGCGCGTA+CGG	+	chr8_2:57191460-57191479	Msa1256910:CDS	55.0%
	CTGCCATACTTGGGCTTCCA+TGG	-	chr8_2:57191495-57191514	None:intergenic	55.0%
	CATGGAAGCCCAAGTATGGC+AGG	+	chr8_2:57191493-57191512	Msa1256910:CDS	55.0%
!	GGGCTTTTGCCTGCCATACT+TGG	-	chr8_2:57191505-57191524	None:intergenic	55.0%
	AGTATGGCAGGCAAAAGCCC+GGG	+	chr8_2:57191505-57191524	Msa1256910:CDS	55.0%
	CTGGGCTTGTAGAGAAGCTG+CGG	-	chr8_2:57191543-57191562	None:intergenic	55.0%
	TCTCAAACCGGCGAAGATGC+CGG	+	chr8_2:57191581-57191600	Msa1256910:CDS	55.0%
!	TAGAGTGGTGGAAGTTGCGG+CGG	+	chr8_2:57191673-57191692	Msa1256910:CDS	55.0%
	ACTAAACGCGGCGGAGGAAG+CGG	+	chr8_2:57191382-57191401	Msa1256910:CDS	60.0%
	GCGTACGGCCCAGTTAACCA+TGG	+	chr8_2:57191475-57191494	Msa1256910:CDS	60.0%
	AGAAGCTGCGGCGGCTTCTT+GGG	-	chr8_2:57191531-57191550	None:intergenic	60.0%
	GGCTTGTAGAGAAGCTGCGG+CGG	-	chr8_2:57191540-57191559	None:intergenic	60.0%
	CGGCGCGTGGGAAATGTAGA+GGG	+	chr8_2:57191693-57191712	Msa1256910:CDS	60.0%
!	TAGAGGGTGTGAGAAGCGCG+TGG	+	chr8_2:57191709-57191728	Msa1256910:CDS	60.0%
!	GTGTGAGAAGCGCGTGGCAA+CGG	+	chr8_2:57191715-57191734	Msa1256910:CDS	60.0%
	ACTAAACGCGGCGGAGGAAG+CGG	+	chr8_2:57191382-57191401	Msa1256910:CDS	60.0%
	GCGTACGGCCCAGTTAACCA+TGG	+	chr8_2:57191475-57191494	Msa1256910:CDS	60.0%
	AGAAGCTGCGGCGGCTTCTT+GGG	-	chr8_2:57191531-57191550	None:intergenic	60.0%
	GGCTTGTAGAGAAGCTGCGG+CGG	-	chr8_2:57191540-57191559	None:intergenic	60.0%
	CGGCGCGTGGGAAATGTAGA+GGG	+	chr8_2:57191693-57191712	Msa1256910:CDS	60.0%
!	TAGAGGGTGTGAGAAGCGCG+TGG	+	chr8_2:57191709-57191728	Msa1256910:CDS	60.0%
!	GTGTGAGAAGCGCGTGGCAA+CGG	+	chr8_2:57191715-57191734	Msa1256910:CDS	60.0%
	GGCGGACATTGGCGGAGAAG+AGG	+	chr8_2:57191339-57191358	Msa1256910:CDS	65.0%
	CCGCCGCGTTTAGTAGCTCC+CGG	-	chr8_2:57191376-57191395	None:intergenic	65.0%
	CCGGGAGCTACTAAACGCGG+CGG	+	chr8_2:57191373-57191392	Msa1256910:CDS	65.0%
	GGAGCTACTAAACGCGGCGG+AGG	+	chr8_2:57191376-57191395	Msa1256910:CDS	65.0%
	GAGAAGCTGCGGCGGCTTCT+TGG	-	chr8_2:57191532-57191551	None:intergenic	65.0%
	CAAACCGGCGAAGATGCCGG+CGG	+	chr8_2:57191584-57191603	Msa1256910:CDS	65.0%
	GGCGTTTCATGCGGCGTGGA+AGG	+	chr8_2:57191611-57191630	Msa1256910:CDS	65.0%
	CGGCGTGGAAGGACAAGCTG+AGG	+	chr8_2:57191622-57191641	Msa1256910:CDS	65.0%
	GCGGCGCGTGGGAAATGTAG+AGG	+	chr8_2:57191692-57191711	Msa1256910:CDS	65.0%
!	TGAGAAGCGCGTGGCAACGG+TGG	+	chr8_2:57191718-57191737	Msa1256910:CDS	65.0%
	GGCGGACATTGGCGGAGAAG+AGG	+	chr8_2:57191339-57191358	Msa1256910:CDS	65.0%
	CCGCCGCGTTTAGTAGCTCC+CGG	-	chr8_2:57191376-57191395	None:intergenic	65.0%
	CCGGGAGCTACTAAACGCGG+CGG	+	chr8_2:57191373-57191392	Msa1256910:CDS	65.0%
	GGAGCTACTAAACGCGGCGG+AGG	+	chr8_2:57191376-57191395	Msa1256910:CDS	65.0%
	GAGAAGCTGCGGCGGCTTCT+TGG	-	chr8_2:57191532-57191551	None:intergenic	65.0%
	CAAACCGGCGAAGATGCCGG+CGG	+	chr8_2:57191584-57191603	Msa1256910:CDS	65.0%
	GGCGTTTCATGCGGCGTGGA+AGG	+	chr8_2:57191611-57191630	Msa1256910:CDS	65.0%
	CGGCGTGGAAGGACAAGCTG+AGG	+	chr8_2:57191622-57191641	Msa1256910:CDS	65.0%
	GCGGCGCGTGGGAAATGTAG+AGG	+	chr8_2:57191692-57191711	Msa1256910:CDS	65.0%
!	TGAGAAGCGCGTGGCAACGG+TGG	+	chr8_2:57191718-57191737	Msa1256910:CDS	65.0%
	AAACGCGGCGGAGGAAGCGG+TGG	+	chr8_2:57191385-57191404	Msa1256910:CDS	70.0%
	GCATGAAACGCCGCCTCCGC+CGG	-	chr8_2:57191603-57191622	None:intergenic	70.0%
	GGCGGAGGCGGCGTTTCATG+CGG	+	chr8_2:57191602-57191621	Msa1256910:CDS	70.0%
	AGGCGGCGTTTCATGCGGCG+TGG	+	chr8_2:57191607-57191626	Msa1256910:CDS	70.0%
	TGGAAGTTGCGGCGGCGCGT+GGG	+	chr8_2:57191681-57191700	Msa1256910:CDS	70.0%
	AAACGCGGCGGAGGAAGCGG+TGG	+	chr8_2:57191385-57191404	Msa1256910:CDS	70.0%
	GCATGAAACGCCGCCTCCGC+CGG	-	chr8_2:57191603-57191622	None:intergenic	70.0%
	GGCGGAGGCGGCGTTTCATG+CGG	+	chr8_2:57191602-57191621	Msa1256910:CDS	70.0%
	AGGCGGCGTTTCATGCGGCG+TGG	+	chr8_2:57191607-57191626	Msa1256910:CDS	70.0%
	TGGAAGTTGCGGCGGCGCGT+GGG	+	chr8_2:57191681-57191700	Msa1256910:CDS	70.0%
	CGGAGGAAGCGGTGGCGCAC+CGG	+	chr8_2:57191393-57191412	Msa1256910:CDS	75.0%
	TGCGGCGGCTTCTTGGGCCC+GGG	-	chr8_2:57191525-57191544	None:intergenic	75.0%
	CTGCGGCGGCTTCTTGGGCC+CGG	-	chr8_2:57191526-57191545	None:intergenic	75.0%
	ACCGGCGAAGATGCCGGCGG+AGG	+	chr8_2:57191587-57191606	Msa1256910:CDS	75.0%
	GCCTCCGCCGGCATCTTCGC+CGG	-	chr8_2:57191591-57191610	None:intergenic	75.0%
	GGCGAAGATGCCGGCGGAGG+CGG	+	chr8_2:57191590-57191609	Msa1256910:CDS	75.0%
	GTGGAAGTTGCGGCGGCGCG+TGG	+	chr8_2:57191680-57191699	Msa1256910:CDS	75.0%
	CGGAGGAAGCGGTGGCGCAC+CGG	+	chr8_2:57191393-57191412	Msa1256910:CDS	75.0%
	TGCGGCGGCTTCTTGGGCCC+GGG	-	chr8_2:57191525-57191544	None:intergenic	75.0%
	CTGCGGCGGCTTCTTGGGCC+CGG	-	chr8_2:57191526-57191545	None:intergenic	75.0%
	ACCGGCGAAGATGCCGGCGG+AGG	+	chr8_2:57191587-57191606	Msa1256910:CDS	75.0%
	GCCTCCGCCGGCATCTTCGC+CGG	-	chr8_2:57191591-57191610	None:intergenic	75.0%
	GGCGAAGATGCCGGCGGAGG+CGG	+	chr8_2:57191590-57191609	Msa1256910:CDS	75.0%
	GTGGAAGTTGCGGCGGCGCG+TGG	+	chr8_2:57191680-57191699	Msa1256910:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	57190255	57192138	57190255	ID=Msa1256910;Name=Msa1256910
chr8_2	mRNA	57190255	57192138	57190255	ID=Msa1256910-mRNA-1;Parent=Msa1256910;Name=Msa1256910-mRNA-1;_AED=0.07;_eAED=0.07;_QI=241\|1\|1\|1\|1\|1\|4\|285\|341
chr8_2	exon	57190255	57190557	57190255	ID=Msa1256910-mRNA-1:exon:17647;Parent=Msa1256910-mRNA-1
chr8_2	exon	57190674	57190798	57190674	ID=Msa1256910-mRNA-1:exon:17648;Parent=Msa1256910-mRNA-1
chr8_2	exon	57190895	57191205	57190895	ID=Msa1256910-mRNA-1:exon:17649;Parent=Msa1256910-mRNA-1
chr8_2	exon	57191326	57192138	57191326	ID=Msa1256910-mRNA-1:exon:17650;Parent=Msa1256910-mRNA-1
chr8_2	five_prime_UTR	57190255	57190495	57190255	ID=Msa1256910-mRNA-1:five_prime_utr;Parent=Msa1256910-mRNA-1
chr8_2	CDS	57190496	57190557	57190496	ID=Msa1256910-mRNA-1:cds;Parent=Msa1256910-mRNA-1
chr8_2	CDS	57190674	57190798	57190674	ID=Msa1256910-mRNA-1:cds;Parent=Msa1256910-mRNA-1
chr8_2	CDS	57190895	57191205	57190895	ID=Msa1256910-mRNA-1:cds;Parent=Msa1256910-mRNA-1
chr8_2	CDS	57191326	57191853	57191326	ID=Msa1256910-mRNA-1:cds;Parent=Msa1256910-mRNA-1
chr8_2	three_prime_UTR	57191854	57192138	57191854	ID=Msa1256910-mRNA-1:three_prime_utr;Parent=Msa1256910-mRNA-1

Gene Sequence

>Msa1256910

ATGTCTGTTCAAGCTCAATATCCATCAAATATTCTTCTTCTAAACAACAACAACAATAACAGCAGAAATGGACAAGAAGGGCATGAATACTCTTTACAACAACAACAACCATCAGGAGCATTAGGACTATCACTTCTTGATCAAACTCATCATTCTCATAATATCCTATGCAACAATAACAACACAAGTACAAATTCTCGCAAGAGAGGAAGAGAAGGAACATCCCCTAACGTTATTAATCATTTCTCTTTACAACCTCAACCTTCTCAAATTATTCATCTTTCTCAACTCCATAATGTTGTCTCTACTGGCCTTCGCTTATCATTCGATGATCAACAACAACAAAGATTACAGTTACAACTTCATCAACACCAACAATCTCAACAACAACAACAACAAGGTTGTCACTCTTCATCTTTCTCATCTCTATTACCACAAGGAATTGTTTCTCAAATCAAACAACAACACGATGAATTAGACCAATATCTTCAAACCCAGGGAGAGAATCTACGGCGGACATTGGCGGAGAAGAGGCAAAAACATTACCGGGAGCTACTAAACGCGGCGGAGGAAGCGGTGGCGCACCGGTTAAGAGAGAAAGAAGTGGAATTCGCAAAAGCCACTCGCAGGAACGCGGATTTAGAAGCGCGTACGGCCCAGTTAACCATGGAAGCCCAAGTATGGCAGGCAAAAGCCCGGGCCCAAGAAGCCGCCGCAGCTTCTCTACAAGCCCAGCTGCAACAAACAATAATGTCTCAAACCGGCGAAGATGCCGGCGGAGGCGGCGTTTCATGCGGCGTGGAAGGACAAGCTGAGGATGCTGAATCGGCTTATATTGATCCTGATAGAGTGGTGGAAGTTGCGGCGGCGCGTGGGAAATGTAGAGGGTGTGAGAAGCGCGTGGCAACGGTGGTTGTTTTACCTTGTCGGCATTTGTGTGTTTGTACAGAATGCGATGCGCATTTCAGAGTTTGCCCTGTTTGCTTTACTCCCAAGAATTCAACCGTTGAAGTCTTTCTCTCTTAG

Protein sequence

>Msa1256910

MSVQAQYPSNILLLNNNNNNSRNGQEGHEYSLQQQQPSGALGLSLLDQTHHSHNILCNNNNTSTNSRKRGREGTSPNVINHFSLQPQPSQIIHLSQLHNVVSTGLRLSFDDQQQQRLQLQLHQHQQSQQQQQQGCHSSSFSSLLPQGIVSQIKQQHDELDQYLQTQGENLRRTLAEKRQKHYRELLNAAEEAVAHRLREKEVEFAKATRRNADLEARTAQLTMEAQVWQAKARAQEAAAASLQAQLQQTIMSQTGEDAGGGGVSCGVEGQAEDAESAYIDPDRVVEVAAARGKCRGCEKRVATVVVLPCRHLCVCTECDAHFRVCPVCFTPKNSTVEVFLS*