AlfalfaGEDB | Msa1209990

M. sativa cultivar zhongmu-4 / Msa1209990

KEGG: -
GO: -
PFAM: PF00300.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1209990	G7JPU0	97.333	300	8	0	1	300	1	300	0.0	608

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa1209990	0.882898	7.077305e-71	-8.615850e-47
Msa0000800	Msa1209990	-0.851148	1.071761e-60	-8.615850e-47
Msa0001040	Msa1209990	0.826457	2.685094e-54	-8.615850e-47
Msa0001510	Msa1209990	0.889544	2.202331e-73	-8.615850e-47
Msa0001550	Msa1209990	0.824837	6.500689e-54	-8.615850e-47
Msa0002110	Msa1209990	0.921599	2.910256e-88	-8.615850e-47
Msa0002690	Msa1209990	0.909247	7.031296e-82	-8.615850e-47
Msa0002700	Msa1209990	0.908761	1.200269e-81	-8.615850e-47
Msa0003340	Msa1209990	0.896668	2.958318e-76	-8.615850e-47
Msa0004180	Msa1209990	0.926177	6.708561e-91	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1209990	MtrunA17_Chr4g0059171	97.333	300	8	0	1	300	1	300	0.0	608

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1209990	AT5G62840.1	70.260	269	74	2	32	300	35	297	2.12e-138	393
Msa1209990	AT5G62840.2	76.339	224	53	0	77	300	9	232	2.06e-130	370

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Find 109 sgRNAs with CRISPR-Local

Find 336 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAGTACTATCTTGTG+AGG	0.679514	8_1:-68683259	Msa1209990:CDS
AAACCTCCTCCCATTGATGA+CGG	0.626640	8_1:-68682243	Msa1209990:CDS
AACTTGTCAATCCTTCAGGC+TGG	0.518288	8_1:-68682108	Msa1209990:CDS
AATACAGCTTTCTTGATGCC+CGG	0.469191	8_1:-68682900	Msa1209990:CDS
AATGCATGGAATAGAGGTGA+CGG	0.617806	8_1:-68681592	Msa1209990:three_prime_UTR
AATGGCTACTGGGTCTGAAA+TGG	0.406139	8_1:+68683319	None:intergenic
ACATCTGCAACACTCTCATT+TGG	0.372094	8_1:+68682216	None:intergenic
ACCTTATTATACCAGATAAA+TGG	0.215021	8_1:-68681793	Msa1209990:three_prime_UTR
AGAATCAGAGTTTGAGAGTA+TGG	0.209979	8_1:-68683231	Msa1209990:CDS
AGATAAACCATTATCCACAG+AGG	0.721820	8_1:+68683178	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTCATCGTTGCTTATGATA+AGG	-	chr8_1:68681199-68681218	Msa1209990:three_prime_UTR	30.0%
GTGCTTGTGATAATAATTGT+TGG	-	chr8_1:68681660-68681679	Msa1209990:three_prime_UTR	30.0%
TTACTAGAAGGTTGTTATGT+TGG	-	chr8_1:68681747-68681766	Msa1209990:three_prime_UTR	30.0%
ATATATAATTGCAGAGGCTT+TGG	+	chr8_1:68682744-68682763	None:intergenic	30.0%
TTACTAGTGTCTCAAATTTC+AGG	-	chr8_1:68682811-68682830	Msa1209990:intron	30.0%
TATCTGGTATAATAAGGTGA+AGG	+	chr8_1:68682968-68682987	None:intergenic	30.0%
ATCAAGATATATGCTATAGC+AGG	-	chr8_1:68683237-68683256	Msa1209990:CDS	30.0%
AGATTACTTGTCTTTCAGTT+TGG	-	chr8_1:68683396-68683415	Msa1209990:CDS	30.0%
TTTGAGCTACATTGTGTAAA+TGG	+	chr8_1:68683528-68683547	None:intergenic	30.0%
TTGAACCCTCTCTTATTAAT+TGG	-	chr8_1:68683549-68683568	Msa1209990:five_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	68681173	68683612	68681173	ID=Msa1209990;Name=Msa1209990
chr8_1	mRNA	68681173	68683612	68681173	ID=Msa1209990-mRNA-1;Parent=Msa1209990;Name=Msa1209990-mRNA-1;_AED=0.02;_eAED=0.02;_QI=99\|1\|1\|1\|1\|1\|4\|659\|300
chr8_1	exon	68681173	68681952	68681173	ID=Msa1209990-mRNA-1:exon:22870;Parent=Msa1209990-mRNA-1
chr8_1	exon	68682084	68682298	68682084	ID=Msa1209990-mRNA-1:exon:22869;Parent=Msa1209990-mRNA-1
chr8_1	exon	68682855	68682936	68682855	ID=Msa1209990-mRNA-1:exon:22868;Parent=Msa1209990-mRNA-1
chr8_1	exon	68683029	68683612	68683029	ID=Msa1209990-mRNA-1:exon:22867;Parent=Msa1209990-mRNA-1
chr8_1	five_prime_UTR	68683514	68683612	68683514	ID=Msa1209990-mRNA-1:five_prime_utr;Parent=Msa1209990-mRNA-1
chr8_1	CDS	68683029	68683513	68683029	ID=Msa1209990-mRNA-1:cds;Parent=Msa1209990-mRNA-1
chr8_1	CDS	68682855	68682936	68682855	ID=Msa1209990-mRNA-1:cds;Parent=Msa1209990-mRNA-1
chr8_1	CDS	68682084	68682298	68682084	ID=Msa1209990-mRNA-1:cds;Parent=Msa1209990-mRNA-1
chr8_1	CDS	68681832	68681952	68681832	ID=Msa1209990-mRNA-1:cds;Parent=Msa1209990-mRNA-1
chr8_1	three_prime_UTR	68681173	68681831	68681173	ID=Msa1209990-mRNA-1:three_prime_utr;Parent=Msa1209990-mRNA-1

Gene Sequence

>Msa1209990

ATGCTATTAGCACCAAATAACACGTGTCAAACCCTTTCATTTTCTCAGCCAGAACAACCACCATGTCTTCCTCCCAAACAACTCAACAACCTCCTCCACCGTCGCCACCTTCTCACCACAACCGCATTCACCATCTCCTTCAACTTCAACCTCACTTCAAAAACCACACTTCCCATTTCAGACCCAGTAGCCATTGCCAGTGGACTCTTCATCATGCCTCCACCACGCCTTAAAAACCAGTACTATCTTGTGAGGTCTGGAGAATCAGAGTTTGAGAGTATGGGAGTGATAAACACCAACCCTGTGGCGAAAACCTCTGTGGATAATGGTTTATCTGATAGAGGGAAGAAACAGAGTATCAGAGCTGCTTTTGATTTGAAAGAAATGGGTGCTTGTGATAATAATTGTTGGATTTGGCCTGCTATTACTCAGAGAGCTTATCAAACTGCTGAGATTATTGCTTCTGTTAATTCTATTACTAGAAGTTATATTGTTCCGGAATACAGCTTTCTTGATGCCCGGGGATTAGGTGCTTATGAAGGAAAAACTTTAGAATATGTTTCAGAAATATATGCATCAGATGGTATATCTACAAAAATAAAACCTCCTCCCATTGATGACGGAACTCCAAATGAGAGTGTTGCAGATGTTTTTGTTCGTGTGACCCAACTCATGTCAATCCTTGAAACTCAATACGCTGGTGACACAGTCGTTATAGTCTCGCCAGATTCGGATAACTTGTCAATCCTTCAGGCTGGTTTGGTAGGCCTAGACTTGCGAAGGCACAGGGAGTTGTCTTTTGCACCTGGTGAAGTTCGCCAGGTTGATACCAATGACATTCCTACTTACAAGATGCCTCCATCTGCTGTATATAAATGCTCCAAGCTACCAAATTGTAATTAG

Protein sequence

>Msa1209990

MLLAPNNTCQTLSFSQPEQPPCLPPKQLNNLLHRRHLLTTTAFTISFNFNLTSKTTLPISDPVAIASGLFIMPPPRLKNQYYLVRSGESEFESMGVINTNPVAKTSVDNGLSDRGKKQSIRAAFDLKEMGACDNNCWIWPAITQRAYQTAEIIASVNSITRSYIVPEYSFLDARGLGAYEGKTLEYVSEIYASDGISTKIKPPPIDDGTPNESVADVFVRVTQLMSILETQYAGDTVVIVSPDSDNLSILQAGLVGLDLRRHRELSFAPGEVRQVDTNDIPTYKMPPSAVYKCSKLPNCN*