AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0976250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0976250 MtrunA17_Chr6g0455241 97.701 87 2 0 19 105 56 142 2.32e-60 181
Msa0976250 MtrunA17_Chr6g0455271 95.833 96 4 0 1 96 2 97 2.48e-52 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGCTTGCCTTCTCAAAA+TGG 0.248342 6_4:-10141201 Msa0976250:CDS
TCATGCAGCAAGAATGCTTT+TGG 0.265450 6_4:-10141304 Msa0976250:CDS
ACAACATCATTGAAGATAAA+AGG 0.287321 6_4:-10141390 Msa0976250:CDS
TTGTCTGATCAACAATGATT+TGG 0.352096 6_4:-10141352 Msa0976250:CDS
TAAGTACCACACCTATTGTT+AGG 0.386678 6_4:+10141171 None:intergenic
GTACGATAGCCTTGACGTAT+TGG 0.444086 6_4:+10141222 None:intergenic
CAAACACAAACTGGTAGAAC+TGG 0.449515 6_4:-10141273 Msa0976250:CDS
GATTTGAGTCAAACACAAAC+TGG 0.464256 6_4:-10141282 Msa0976250:CDS
ATGGAGGTGGACCTAACAAT+AGG 0.486497 6_4:-10141182 Msa0976250:CDS
GTAATTGGATCAATAAGATA+AGG 0.536107 6_4:+10141424 None:intergenic
TTGCCTTCTCAAAATGGAGG+TGG 0.550109 6_4:-10141195 Msa0976250:CDS
GCCAAAGAAGAAAATGAACA+AGG 0.614392 6_4:-10141463 None:intergenic
TGCTTGCCTTCTCAAAATGG+AGG 0.620754 6_4:-10141198 Msa0976250:CDS
GGTAGAACTGGTAATAACAA+TGG 0.632835 6_4:-10141261 Msa0976250:CDS
GTTAAGTGTCCAATACGTCA+AGG 0.658926 6_4:-10141231 Msa0976250:CDS
GATAAAAGGTTGTACCTCAG+AGG 0.662136 6_4:-10141376 Msa0976250:CDS
CCAAAGAAGAAAATGAACAA+GGG 0.665121 6_4:-10141462 None:intergenic
GGTGGACCTAACAATAGGTG+TGG 0.694293 6_4:-10141177 Msa0976250:CDS
GTTGATCAGACAATCCTCTG+AGG 0.737752 6_4:+10141362 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTAATTGGATCAATAAGATA+AGG + chr6_4:10141184-10141203 None:intergenic 25.0%
!!! TTGTTTTGCTTAGATGTAAT+TGG + chr6_4:10141199-10141218 None:intergenic 25.0%
! ACAACATCATTGAAGATAAA+AGG - chr6_4:10141215-10141234 Msa0976250:CDS 25.0%
TTGTCTGATCAACAATGATT+TGG - chr6_4:10141253-10141272 Msa0976250:CDS 30.0%
GATTTGAGTCAAACACAAAC+TGG - chr6_4:10141323-10141342 Msa0976250:CDS 35.0%
GGTAGAACTGGTAATAACAA+TGG - chr6_4:10141344-10141363 Msa0976250:CDS 35.0%
TAAGTACCACACCTATTGTT+AGG + chr6_4:10141437-10141456 None:intergenic 35.0%
GATAAAAGGTTGTACCTCAG+AGG - chr6_4:10141229-10141248 Msa0976250:CDS 40.0%
! TCATGCAGCAAGAATGCTTT+TGG - chr6_4:10141301-10141320 Msa0976250:CDS 40.0%
CAAACACAAACTGGTAGAAC+TGG - chr6_4:10141332-10141351 Msa0976250:CDS 40.0%
GTTAAGTGTCCAATACGTCA+AGG - chr6_4:10141374-10141393 Msa0976250:CDS 40.0%
ACTTGCTTGCCTTCTCAAAA+TGG - chr6_4:10141404-10141423 Msa0976250:CDS 40.0%
GTTGATCAGACAATCCTCTG+AGG + chr6_4:10141246-10141265 None:intergenic 45.0%
! GTACGATAGCCTTGACGTAT+TGG + chr6_4:10141386-10141405 None:intergenic 45.0%
TGCTTGCCTTCTCAAAATGG+AGG - chr6_4:10141407-10141426 Msa0976250:CDS 45.0%
TTGCCTTCTCAAAATGGAGG+TGG - chr6_4:10141410-10141429 Msa0976250:CDS 45.0%
ATGGAGGTGGACCTAACAAT+AGG - chr6_4:10141423-10141442 Msa0976250:CDS 45.0%
!! GGTCCACCTCCATTTTGAGA+AGG + chr6_4:10141416-10141435 None:intergenic 50.0%
GGTGGACCTAACAATAGGTG+TGG - chr6_4:10141428-10141447 Msa0976250:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 10141155 10141472 10141155 ID=Msa0976250;Name=Msa0976250
chr6_4 mRNA 10141155 10141472 10141155 ID=Msa0976250-mRNA-1;Parent=Msa0976250;Name=Msa0976250-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|105
chr6_4 exon 10141155 10141472 10141155 ID=Msa0976250-mRNA-1:exon:3732;Parent=Msa0976250-mRNA-1
chr6_4 CDS 10141155 10141472 10141155 ID=Msa0976250-mRNA-1:cds;Parent=Msa0976250-mRNA-1
Gene Sequence

>Msa0976250

ATGAACAAGGGTGTATCACTATTGTTCCTTATCTTATTGATCCAATTACATCTAAGCAAAACAACATCATTGAAGATAAAAGGTTGTACCTCAGAGGATTGTCTGATCAACAATGATTTGGAATCAGAAGAATTTTCTATGAGTTCTCATGCAGCAAGAATGCTTTTGGATTTGAGTCAAACACAAACTGGTAGAACTGGTAATAACAATGGTGCTGCTGTTAAGTGTCCAATACGTCAAGGCTATCGTACTTGCTTGCCTTCTCAAAATGGAGGTGGACCTAACAATAGGTGTGGTACTTATAATAGAGTTTGCTAA

Protein sequence

>Msa0976250

MNKGVSLLFLILLIQLHLSKTTSLKIKGCTSEDCLINNDLESEEFSMSSHAARMLLDLSQTQTGRTGNNNGAAVKCPIRQGYRTCLPSQNGGGPNNRCGTYNRVC*