Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41634.t1 | XP_003602738.2 | 95.4 | 216 | 10 | 0 | 1 | 216 | 7 | 222 | 1.50E-119 | 438.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41634.t1 | Q67YC9 | 61.3 | 181 | 63 | 4 | 11 | 188 | 7 | 183 | 7.3e-62 | 238.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41634.t1 | G7J2S4 | 95.4 | 216 | 10 | 0 | 1 | 216 | 7 | 222 | 1.1e-119 | 438.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene41634 | 0.804448 | 2.15E-49 | -1.69E-46 |
| MS.gene049171 | MS.gene41634 | 0.878935 | 1.88E-69 | -1.69E-46 |
| MS.gene049173 | MS.gene41634 | 0.857417 | 1.66E-62 | -1.69E-46 |
| MS.gene049217 | MS.gene41634 | 0.855253 | 7.14E-62 | -1.69E-46 |
| MS.gene049222 | MS.gene41634 | 0.860638 | 1.80E-63 | -1.69E-46 |
| MS.gene049285 | MS.gene41634 | 0.90405 | 1.84E-79 | -1.69E-46 |
| MS.gene049320 | MS.gene41634 | 0.879668 | 1.03E-69 | -1.69E-46 |
| MS.gene049427 | MS.gene41634 | 0.838608 | 2.60E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene41634 | 0.86228 | 5.67E-64 | -1.69E-46 |
| MS.gene049633 | MS.gene41634 | 0.859936 | 2.93E-63 | -1.69E-46 |
| MS.gene049805 | MS.gene41634 | 0.839205 | 1.82E-57 | -1.69E-46 |
| MS.gene05010 | MS.gene41634 | 0.827379 | 1.62E-54 | -1.69E-46 |
| MS.gene050163 | MS.gene41634 | 0.869461 | 3.04E-66 | -1.69E-46 |
| MS.gene050165 | MS.gene41634 | 0.864441 | 1.21E-64 | -1.69E-46 |
| MS.gene050323 | MS.gene41634 | 0.828919 | 6.88E-55 | -1.69E-46 |
| MS.gene050333 | MS.gene41634 | 0.862127 | 6.31E-64 | -1.69E-46 |
| MS.gene050432 | MS.gene41634 | 0.823281 | 1.51E-53 | -1.69E-46 |
| MS.gene050444 | MS.gene41634 | 0.846913 | 1.61E-59 | -1.69E-46 |
| MS.gene050504 | MS.gene41634 | 0.86157 | 9.35E-64 | -1.69E-46 |
| MS.gene050518 | MS.gene41634 | 0.870217 | 1.72E-66 | -1.69E-46 |
| MS.gene050538 | MS.gene41634 | 0.840272 | 9.61E-58 | -1.69E-46 |
| MS.gene050577 | MS.gene41634 | 0.813295 | 2.75E-51 | -1.69E-46 |
| MS.gene050593 | MS.gene41634 | 0.829136 | 6.09E-55 | -1.69E-46 |
| MS.gene050612 | MS.gene41634 | 0.890669 | 7.99E-74 | -1.69E-46 |
| MS.gene050675 | MS.gene41634 | 0.862934 | 3.56E-64 | -1.69E-46 |
| MS.gene050699 | MS.gene41634 | 0.867241 | 1.58E-65 | -1.69E-46 |
| MS.gene050747 | MS.gene41634 | 0.842001 | 3.38E-58 | -1.69E-46 |
| MS.gene050767 | MS.gene41634 | 0.818864 | 1.57E-52 | -1.69E-46 |
| MS.gene050770 | MS.gene41634 | 0.824785 | 6.69E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene41634 | 0.888532 | 5.43E-73 | -1.69E-46 |
| MS.gene05080 | MS.gene41634 | 0.852611 | 4.12E-61 | -1.69E-46 |
| MS.gene05081 | MS.gene41634 | 0.841087 | 5.88E-58 | -1.69E-46 |
| MS.gene050868 | MS.gene41634 | 0.824069 | 9.85E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene41634 | 0.821129 | 4.76E-53 | -1.69E-46 |
| MS.gene050950 | MS.gene41634 | 0.848341 | 6.52E-60 | -1.69E-46 |
| MS.gene050986 | MS.gene41634 | 0.862628 | 4.43E-64 | -1.69E-46 |
| MS.gene05100 | MS.gene41634 | 0.831024 | 2.11E-55 | -1.69E-46 |
| MS.gene05105 | MS.gene41634 | 0.812488 | 4.13E-51 | -1.69E-46 |
| MS.gene051228 | MS.gene41634 | 0.893481 | 6.04E-75 | -1.69E-46 |
| MS.gene051317 | MS.gene41634 | 0.859041 | 5.44E-63 | -1.69E-46 |
| MS.gene051387 | MS.gene41634 | 0.803321 | 3.69E-49 | -1.69E-46 |
| MS.gene051434 | MS.gene41634 | 0.836551 | 8.76E-57 | -1.69E-46 |
| MS.gene051551 | MS.gene41634 | 0.825606 | 4.28E-54 | -1.69E-46 |
| MS.gene051617 | MS.gene41634 | 0.874306 | 7.49E-68 | -1.69E-46 |
| MS.gene051666 | MS.gene41634 | 0.844041 | 9.66E-59 | -1.69E-46 |
| MS.gene051694 | MS.gene41634 | 0.800644 | 1.31E-48 | -1.69E-46 |
| MS.gene051734 | MS.gene41634 | 0.804064 | 2.58E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene41634 | 0.901374 | 2.86E-78 | -1.69E-46 |
| MS.gene051838 | MS.gene41634 | 0.840187 | 1.01E-57 | -1.69E-46 |
| MS.gene051858 | MS.gene41634 | 0.836968 | 6.86E-57 | -1.69E-46 |
| MS.gene051949 | MS.gene41634 | 0.851007 | 1.17E-60 | -1.69E-46 |
| MS.gene052164 | MS.gene41634 | 0.812061 | 5.12E-51 | -1.69E-46 |
| MS.gene052271 | MS.gene41634 | 0.854699 | 1.03E-61 | -1.69E-46 |
| MS.gene052290 | MS.gene41634 | 0.821213 | 4.55E-53 | -1.69E-46 |
| MS.gene052379 | MS.gene41634 | 0.873654 | 1.24E-67 | -1.69E-46 |
| MS.gene052484 | MS.gene41634 | 0.835319 | 1.80E-56 | -1.69E-46 |
| MS.gene052503 | MS.gene41634 | 0.800774 | 1.23E-48 | -1.69E-46 |
| MS.gene052506 | MS.gene41634 | 0.81113 | 8.16E-51 | -1.69E-46 |
| MS.gene052615 | MS.gene41634 | 0.843742 | 1.16E-58 | -1.69E-46 |
| MS.gene052822 | MS.gene41634 | 0.830487 | 2.86E-55 | -1.69E-46 |
| MS.gene052870 | MS.gene41634 | 0.892516 | 1.48E-74 | -1.69E-46 |
| MS.gene052872 | MS.gene41634 | 0.893186 | 7.94E-75 | -1.69E-46 |
| MS.gene052916 | MS.gene41634 | 0.812926 | 3.32E-51 | -1.69E-46 |
| MS.gene052966 | MS.gene41634 | 0.836107 | 1.14E-56 | -1.69E-46 |
| MS.gene053009 | MS.gene41634 | 0.853614 | 2.13E-61 | -1.69E-46 |
| MS.gene053103 | MS.gene41634 | 0.81211 | 5.00E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene41634 | 0.856261 | 3.63E-62 | -1.69E-46 |
| MS.gene053107 | MS.gene41634 | 0.827203 | 1.78E-54 | -1.69E-46 |
| MS.gene053117 | MS.gene41634 | 0.801717 | 7.89E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene41634 | 0.837601 | 4.72E-57 | -1.69E-46 |
| MS.gene053207 | MS.gene41634 | 0.884679 | 1.56E-71 | -1.69E-46 |
| MS.gene053209 | MS.gene41634 | 0.803461 | 3.45E-49 | -1.69E-46 |
| MS.gene053213 | MS.gene41634 | 0.875589 | 2.73E-68 | -1.69E-46 |
| MS.gene053284 | MS.gene41634 | 0.804105 | 2.53E-49 | -1.69E-46 |
| MS.gene053285 | MS.gene41634 | 0.801414 | 9.11E-49 | -1.69E-46 |
| MS.gene053286 | MS.gene41634 | 0.875168 | 3.81E-68 | -1.69E-46 |
| MS.gene053322 | MS.gene41634 | 0.83925 | 1.77E-57 | -1.69E-46 |
| MS.gene053479 | MS.gene41634 | 0.834779 | 2.46E-56 | -1.69E-46 |
| MS.gene053480 | MS.gene41634 | 0.810346 | 1.21E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene41634 | 0.841467 | 4.67E-58 | -1.69E-46 |
| MS.gene053632 | MS.gene41634 | 0.860805 | 1.60E-63 | -1.69E-46 |
| MS.gene053824 | MS.gene41634 | 0.84869 | 5.22E-60 | -1.69E-46 |
| MS.gene053916 | MS.gene41634 | 0.858408 | 8.41E-63 | -1.69E-46 |
| MS.gene054376 | MS.gene41634 | 0.806769 | 7.00E-50 | -1.69E-46 |
| MS.gene054408 | MS.gene41634 | 0.817328 | 3.49E-52 | -1.69E-46 |
| MS.gene054595 | MS.gene41634 | 0.824088 | 9.75E-54 | -1.69E-46 |
| MS.gene054600 | MS.gene41634 | 0.8939 | 4.09E-75 | -1.69E-46 |
| MS.gene054642 | MS.gene41634 | 0.904665 | 9.68E-80 | -1.69E-46 |
| MS.gene05470 | MS.gene41634 | 0.806861 | 6.70E-50 | -1.69E-46 |
| MS.gene05494 | MS.gene41634 | 0.819256 | 1.28E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene41634 | 0.876388 | 1.45E-68 | -1.69E-46 |
| MS.gene05505 | MS.gene41634 | 0.833925 | 4.03E-56 | -1.69E-46 |
| MS.gene055206 | MS.gene41634 | 0.88174 | 1.87E-70 | -1.69E-46 |
| MS.gene055212 | MS.gene41634 | 0.83398 | 3.91E-56 | -1.69E-46 |
| MS.gene055224 | MS.gene41634 | 0.840367 | 9.08E-58 | -1.69E-46 |
| MS.gene055254 | MS.gene41634 | 0.801877 | 7.32E-49 | -1.69E-46 |
| MS.gene055289 | MS.gene41634 | 0.83757 | 4.81E-57 | -1.69E-46 |
| MS.gene055362 | MS.gene41634 | 0.874891 | 4.74E-68 | -1.69E-46 |
| MS.gene055397 | MS.gene41634 | 0.835505 | 1.61E-56 | -1.69E-46 |
| MS.gene055412 | MS.gene41634 | 0.803463 | 3.44E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene41634 | 0.81963 | 1.05E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene41634 | 0.868842 | 4.83E-66 | -1.69E-46 |
| MS.gene055577 | MS.gene41634 | 0.870417 | 1.48E-66 | -1.69E-46 |
| MS.gene055636 | MS.gene41634 | 0.852485 | 4.48E-61 | -1.69E-46 |
| MS.gene055638 | MS.gene41634 | 0.840359 | 9.12E-58 | -1.69E-46 |
| MS.gene055647 | MS.gene41634 | 0.842542 | 2.43E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene41634 | 0.861427 | 1.03E-63 | -1.69E-46 |
| MS.gene055748 | MS.gene41634 | 0.858768 | 6.56E-63 | -1.69E-46 |
| MS.gene055750 | MS.gene41634 | 0.82403 | 1.01E-53 | -1.69E-46 |
| MS.gene055751 | MS.gene41634 | 0.854529 | 1.16E-61 | -1.69E-46 |
| MS.gene055776 | MS.gene41634 | 0.813528 | 2.45E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene41634 | 0.813485 | 2.50E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene41634 | 0.887754 | 1.08E-72 | -1.69E-46 |
| MS.gene055941 | MS.gene41634 | 0.848444 | 6.11E-60 | -1.69E-46 |
| MS.gene055956 | MS.gene41634 | -0.822337 | 2.50E-53 | -1.69E-46 |
| MS.gene056083 | MS.gene41634 | 0.8345 | 2.89E-56 | -1.69E-46 |
| MS.gene056374 | MS.gene41634 | 0.857689 | 1.38E-62 | -1.69E-46 |
| MS.gene056449 | MS.gene41634 | 0.832591 | 8.66E-56 | -1.69E-46 |
| MS.gene056487 | MS.gene41634 | 0.804261 | 2.35E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene41634 | 0.83436 | 3.14E-56 | -1.69E-46 |
| MS.gene056510 | MS.gene41634 | 0.866889 | 2.05E-65 | -1.69E-46 |
| MS.gene056657 | MS.gene41634 | 0.888227 | 7.11E-73 | -1.69E-46 |
| MS.gene056692 | MS.gene41634 | 0.842174 | 3.04E-58 | -1.69E-46 |
| MS.gene056897 | MS.gene41634 | 0.863688 | 2.08E-64 | -1.69E-46 |
| MS.gene056898 | MS.gene41634 | 0.856225 | 3.71E-62 | -1.69E-46 |
| MS.gene056947 | MS.gene41634 | 0.828435 | 9.00E-55 | -1.69E-46 |
| MS.gene057011 | MS.gene41634 | 0.827749 | 1.32E-54 | -1.69E-46 |
| MS.gene057076 | MS.gene41634 | 0.840543 | 8.16E-58 | -1.69E-46 |
| MS.gene057129 | MS.gene41634 | 0.864273 | 1.37E-64 | -1.69E-46 |
| MS.gene057196 | MS.gene41634 | 0.807961 | 3.91E-50 | -1.69E-46 |
| MS.gene057246 | MS.gene41634 | 0.819791 | 9.65E-53 | -1.69E-46 |
| MS.gene057331 | MS.gene41634 | 0.88769 | 1.14E-72 | -1.69E-46 |
| MS.gene057332 | MS.gene41634 | 0.834698 | 2.58E-56 | -1.69E-46 |
| MS.gene057416 | MS.gene41634 | 0.875969 | 2.03E-68 | -1.69E-46 |
| MS.gene057658 | MS.gene41634 | 0.84893 | 4.48E-60 | -1.69E-46 |
| MS.gene057831 | MS.gene41634 | 0.848771 | 4.96E-60 | -1.69E-46 |
| MS.gene057926 | MS.gene41634 | 0.82071 | 5.94E-53 | -1.69E-46 |
| MS.gene058019 | MS.gene41634 | 0.815722 | 7.99E-52 | -1.69E-46 |
| MS.gene058130 | MS.gene41634 | 0.829948 | 3.87E-55 | -1.69E-46 |
| MS.gene058186 | MS.gene41634 | -0.805218 | 1.48E-49 | -1.69E-46 |
| MS.gene058323 | MS.gene41634 | 0.829797 | 4.21E-55 | -1.69E-46 |
| MS.gene058338 | MS.gene41634 | 0.836836 | 7.41E-57 | -1.69E-46 |
| MS.gene05837 | MS.gene41634 | 0.840417 | 8.80E-58 | -1.69E-46 |
| MS.gene058419 | MS.gene41634 | 0.890222 | 1.20E-73 | -1.69E-46 |
| MS.gene058544 | MS.gene41634 | 0.866717 | 2.33E-65 | -1.69E-46 |
| MS.gene058723 | MS.gene41634 | 0.88514 | 1.05E-71 | -1.69E-46 |
| MS.gene058773 | MS.gene41634 | 0.894307 | 2.79E-75 | -1.69E-46 |
| MS.gene058838 | MS.gene41634 | 0.844626 | 6.73E-59 | -1.69E-46 |
| MS.gene05883 | MS.gene41634 | 0.802782 | 4.76E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene41634 | 0.869371 | 3.26E-66 | -1.69E-46 |
| MS.gene059023 | MS.gene41634 | 0.892269 | 1.86E-74 | -1.69E-46 |
| MS.gene059029 | MS.gene41634 | 0.868188 | 7.86E-66 | -1.69E-46 |
| MS.gene059079 | MS.gene41634 | 0.80996 | 1.46E-50 | -1.69E-46 |
| MS.gene05913 | MS.gene41634 | 0.836716 | 7.95E-57 | -1.69E-46 |
| MS.gene05914 | MS.gene41634 | 0.80783 | 4.17E-50 | -1.69E-46 |
| MS.gene059305 | MS.gene41634 | 0.804637 | 1.96E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene41634 | 0.848836 | 4.75E-60 | -1.69E-46 |
| MS.gene059536 | MS.gene41634 | 0.802231 | 6.19E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene41634 | 0.895868 | 6.37E-76 | -1.69E-46 |
| MS.gene059718 | MS.gene41634 | 0.874894 | 4.73E-68 | -1.69E-46 |
| MS.gene059756 | MS.gene41634 | 0.831892 | 1.29E-55 | -1.69E-46 |
| MS.gene059858 | MS.gene41634 | 0.80571 | 1.17E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene41634 | 0.845172 | 4.79E-59 | -1.69E-46 |
| MS.gene059902 | MS.gene41634 | 0.831053 | 2.08E-55 | -1.69E-46 |
| MS.gene059960 | MS.gene41634 | 0.85261 | 4.12E-61 | -1.69E-46 |
| MS.gene060193 | MS.gene41634 | 0.805805 | 1.12E-49 | -1.69E-46 |
| MS.gene060341 | MS.gene41634 | 0.806483 | 8.05E-50 | -1.69E-46 |
| MS.gene060703 | MS.gene41634 | 0.820371 | 7.11E-53 | -1.69E-46 |
| MS.gene060728 | MS.gene41634 | 0.844081 | 9.43E-59 | -1.69E-46 |
| MS.gene060762 | MS.gene41634 | 0.859652 | 3.57E-63 | -1.69E-46 |
| MS.gene06077 | MS.gene41634 | 0.830498 | 2.84E-55 | -1.69E-46 |
| MS.gene060804 | MS.gene41634 | 0.85531 | 6.87E-62 | -1.69E-46 |
| MS.gene060818 | MS.gene41634 | 0.827009 | 1.98E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene41634 | 0.83232 | 1.01E-55 | -1.69E-46 |
| MS.gene060837 | MS.gene41634 | 0.8102 | 1.30E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene41634 | 0.890228 | 1.19E-73 | -1.69E-46 |
| MS.gene060982 | MS.gene41634 | 0.840185 | 1.01E-57 | -1.69E-46 |
| MS.gene061041 | MS.gene41634 | 0.810011 | 1.42E-50 | -1.69E-46 |
| MS.gene061134 | MS.gene41634 | 0.803539 | 3.32E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene41634 | 0.891891 | 2.62E-74 | -1.69E-46 |
| MS.gene061200 | MS.gene41634 | 0.834524 | 2.85E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41634.t1 | MTR_3g098500 | 95.370 | 216 | 10 | 0 | 1 | 216 | 7 | 222 | 1.97e-156 | 432 |
| MS.gene41634.t1 | MTR_3g098490 | 75.229 | 218 | 46 | 3 | 1 | 216 | 1 | 212 | 9.43e-120 | 339 |
| MS.gene41634.t1 | MTR_8g096110 | 64.737 | 190 | 64 | 2 | 1 | 190 | 1 | 187 | 1.81e-85 | 252 |
| MS.gene41634.t1 | MTR_8g096080 | 64.062 | 192 | 64 | 4 | 1 | 190 | 1 | 189 | 3.34e-84 | 249 |
| MS.gene41634.t1 | MTR_8g096100 | 56.316 | 190 | 80 | 2 | 1 | 190 | 1 | 187 | 3.31e-74 | 223 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41634.t1 | AT3G23760 | 61.828 | 186 | 60 | 4 | 12 | 190 | 9 | 190 | 1.27e-80 | 239 |
| MS.gene41634.t1 | AT4G14100 | 60.656 | 183 | 65 | 4 | 11 | 190 | 7 | 185 | 9.61e-78 | 232 |
Find 55 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACTGTTCATGAGGATTT+TGG | 0.136762 | 3.4:+88362373 | MS.gene41634:CDS |
| CGACAGCATTGAGTTGATTT+TGG | 0.167038 | 3.4:-88361656 | None:intergenic |
| GCTCTTGTTCATAAACATTA+AGG | 0.279361 | 3.4:-88361571 | None:intergenic |
| ATAATTTCCTTTGTAATGTT+TGG | 0.290309 | 3.4:+88361829 | MS.gene41634:CDS |
| CTTGAGGGCGCTACGTATTT+AGG | 0.303379 | 3.4:+88361792 | MS.gene41634:CDS |
| TCCGGCCAAACAGCCGGAAT+TGG | 0.340541 | 3.4:-88361537 | None:intergenic |
| GTAGCGCCCTCAAGAAAATT+AGG | 0.340967 | 3.4:-88361783 | None:intergenic |
| CTGAGCCAATTCCGGCTGTT+TGG | 0.366949 | 3.4:+88361532 | MS.gene41634:CDS |
| TTCGATGGCTGAGCCAATTC+CGG | 0.379483 | 3.4:+88361524 | MS.gene41634:CDS |
| GTTAAGTGGTTCTTCTACTC+AGG | 0.396357 | 3.4:+88361903 | MS.gene41634:CDS |
| GAGAGAAGAGAATGACATAT+AGG | 0.411659 | 3.4:-88361488 | None:intergenic |
| GAGTAGAAGAACCACTTAAC+AGG | 0.415932 | 3.4:-88361900 | None:intergenic |
| GGAGAATACCTACAGGAAAC+TGG | 0.417162 | 3.4:-88361758 | None:intergenic |
| GCCAATTCCGGCTGTTTGGC+CGG | 0.420441 | 3.4:+88361536 | MS.gene41634:CDS |
| CTGCAATAGAACAACTATCT+AGG | 0.426683 | 3.4:+88361460 | MS.gene41634:CDS |
| TCCTGCAGAAAGTGGACTTA+TGG | 0.436493 | 3.4:+88361598 | MS.gene41634:CDS |
| TGTCATTCTCTTCTCTCTCT+CGG | 0.441820 | 3.4:+88361494 | MS.gene41634:CDS |
| GTCTGATTGAAGCAGTATAT+AGG | 0.470699 | 3.4:-88362328 | None:intergenic |
| CGCTCATGTAATGACGTTTG+AGG | 0.471029 | 3.4:+88362270 | MS.gene41634:CDS |
| ATGTTGAAGTTTCGACCGTT+AGG | 0.477068 | 3.4:-88361630 | None:intergenic |
| AACTGCTCCGGCCAAACAGC+CGG | 0.482218 | 3.4:-88361543 | None:intergenic |
| CATGTAATGACGTTTGAGGT+TGG | 0.496845 | 3.4:+88362274 | MS.gene41634:CDS |
| TTGTTCTATTGCAGAGGCCA+TGG | 0.498883 | 3.4:-88361451 | None:intergenic |
| GCTTGAGGAACCGCATTGGC+AGG | 0.504494 | 3.4:+88362303 | MS.gene41634:CDS |
| GGGATGGAGAGTGTAGATGA+AGG | 0.516088 | 3.4:-88361712 | None:intergenic |
| ACCATAAGTCCACTTTCTGC+AGG | 0.523892 | 3.4:-88361599 | None:intergenic |
| TCGTCGTTTACGACCTCGAA+TGG | 0.528379 | 3.4:+88361676 | MS.gene41634:CDS |
| TGCAATGGCGGTCTGAGGGA+TGG | 0.534618 | 3.4:-88361728 | None:intergenic |
| GACCTTGCAATGGCGGTCTG+AGG | 0.545191 | 3.4:-88361733 | None:intergenic |
| TTATGGTACGACTGGCCTAA+CGG | 0.548138 | 3.4:+88361615 | MS.gene41634:CDS |
| GAGCGGAGTCCTGCAGAAAG+TGG | 0.548813 | 3.4:+88361590 | MS.gene41634:CDS |
| CACTGCTTGAGGAACCGCAT+TGG | 0.549270 | 3.4:+88362299 | MS.gene41634:CDS |
| ACCTTGCAATGGCGGTCTGA+GGG | 0.550186 | 3.4:-88361732 | None:intergenic |
| TCACTCGTAGACCTGTTAAG+TGG | 0.552659 | 3.4:+88361889 | MS.gene41634:CDS |
| TCCCTCAGACCGCCATTGCA+AGG | 0.555841 | 3.4:+88361731 | MS.gene41634:CDS |
| ATTAAGGCATGAAACTGCTC+CGG | 0.562441 | 3.4:-88361555 | None:intergenic |
| AGGTACCGTTGGTCCATTCG+AGG | 0.563492 | 3.4:-88361689 | None:intergenic |
| ATGGAGAGTGTAGATGAAGG+AGG | 0.563676 | 3.4:-88361709 | None:intergenic |
| AAGTGGACTTATGGTACGAC+TGG | 0.577314 | 3.4:+88361607 | MS.gene41634:CDS |
| TGAGGTTGGAGCACTGCTTG+AGG | 0.579016 | 3.4:+88362288 | MS.gene41634:CDS |
| AAAATCCTCATGAACAGTAG+TGG | 0.580647 | 3.4:-88362371 | None:intergenic |
| ACTGGACGACCTTGCAATGG+CGG | 0.580698 | 3.4:-88361740 | None:intergenic |
| TCTCTCGGCTTCATATTCGA+TGG | 0.592702 | 3.4:+88361509 | MS.gene41634:CDS |
| CTTTCGTCCAAACATTACAA+AGG | 0.600135 | 3.4:-88361836 | None:intergenic |
| GTAGATGAAGGAGGTACCGT+TGG | 0.604808 | 3.4:-88361700 | None:intergenic |
| CGCCCTCAAGAAAATTAGGA+CGG | 0.606966 | 3.4:-88361779 | None:intergenic |
| AAGGTCGTCCAGTTTCCTGT+AGG | 0.614277 | 3.4:+88361750 | MS.gene41634:CDS |
| AGATAGTTGTTCTATTGCAG+AGG | 0.619269 | 3.4:-88361457 | None:intergenic |
| TACGACCTCGAATGGACCAA+CGG | 0.625467 | 3.4:+88361684 | MS.gene41634:CDS |
| AGAATCCACTACTGTTCATG+AGG | 0.630604 | 3.4:+88362366 | MS.gene41634:CDS |
| TTAGGACGGAGAATACCTAC+AGG | 0.634745 | 3.4:-88361765 | None:intergenic |
| GAAACTGGACGACCTTGCAA+TGG | 0.636092 | 3.4:-88361743 | None:intergenic |
| CTCAAACGTCATTACATGAG+CGG | 0.651282 | 3.4:-88362269 | None:intergenic |
| TTAATGTTTATGAACAAGAG+CGG | 0.658259 | 3.4:+88361573 | MS.gene41634:CDS |
| TATATAGGAGCCTGCCAATG+CGG | 0.702014 | 3.4:-88362313 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAATTAATAAGCTCAAT+CGG | - | chr3.4:88362138-88362157 | None:intergenic | 15.0% |
| !!! | ATAGTGATAATTTTAATAGA+TGG | + | chr3.4:88362238-88362257 | MS.gene41634:intron | 15.0% |
| !! | ATAATTTCCTTTGTAATGTT+TGG | + | chr3.4:88361829-88361848 | MS.gene41634:CDS | 20.0% |
| !!! | TCTCTTATATTATCACTTTT+GGG | + | chr3.4:88361938-88361957 | MS.gene41634:intron | 20.0% |
| ! | ATGACAAGATAAGCTTAAAT+AGG | - | chr3.4:88361979-88361998 | None:intergenic | 25.0% |
| ! | ATTCACTTCATTCAATATCT+TGG | - | chr3.4:88362170-88362189 | None:intergenic | 25.0% |
| ! | TTAATGTTTATGAACAAGAG+CGG | + | chr3.4:88361573-88361592 | MS.gene41634:CDS | 25.0% |
| !! | CTCTCTTATATTATCACTTT+TGG | + | chr3.4:88361937-88361956 | MS.gene41634:intron | 25.0% |
| !! | CTTATCTTGTCATACATTTT+GGG | + | chr3.4:88361986-88362005 | MS.gene41634:intron | 25.0% |
| !!! | GTGATAATTTTAATAGATGG+AGG | + | chr3.4:88362241-88362260 | MS.gene41634:intron | 25.0% |
| GCTCTTGTTCATAAACATTA+AGG | - | chr3.4:88361574-88361593 | None:intergenic | 30.0% | |
| ! | GCTTATCTTGTCATACATTT+TGG | + | chr3.4:88361985-88362004 | MS.gene41634:intron | 30.0% |
| !! | TATCACTTTTGGGCTATATT+TGG | + | chr3.4:88361948-88361967 | MS.gene41634:intron | 30.0% |
| AAAATCCTCATGAACAGTAG+TGG | - | chr3.4:88362374-88362393 | None:intergenic | 35.0% | |
| AGATAGTTGTTCTATTGCAG+AGG | - | chr3.4:88361460-88361479 | None:intergenic | 35.0% | |
| CTGCAATAGAACAACTATCT+AGG | + | chr3.4:88361460-88361479 | MS.gene41634:CDS | 35.0% | |
| CTTTCGTCCAAACATTACAA+AGG | - | chr3.4:88361839-88361858 | None:intergenic | 35.0% | |
| GAGAGAAGAGAATGACATAT+AGG | - | chr3.4:88361491-88361510 | None:intergenic | 35.0% | |
| GTCTGATTGAAGCAGTATAT+AGG | - | chr3.4:88362331-88362350 | None:intergenic | 35.0% | |
| ! | ACTACTGTTCATGAGGATTT+TGG | + | chr3.4:88362373-88362392 | MS.gene41634:CDS | 35.0% |
| ! | CTACTGTTCATGAGGATTTT+GGG | + | chr3.4:88362374-88362393 | MS.gene41634:CDS | 35.0% |
| AGAATCCACTACTGTTCATG+AGG | + | chr3.4:88362366-88362385 | MS.gene41634:CDS | 40.0% | |
| ATGTTGAAGTTTCGACCGTT+AGG | - | chr3.4:88361633-88361652 | None:intergenic | 40.0% | |
| ATTAAGGCATGAAACTGCTC+CGG | - | chr3.4:88361558-88361577 | None:intergenic | 40.0% | |
| CTCAAACGTCATTACATGAG+CGG | - | chr3.4:88362272-88362291 | None:intergenic | 40.0% | |
| GAGTAGAAGAACCACTTAAC+AGG | - | chr3.4:88361903-88361922 | None:intergenic | 40.0% | |
| GTTAAGTGGTTCTTCTACTC+AGG | + | chr3.4:88361903-88361922 | MS.gene41634:CDS | 40.0% | |
| TGTCATTCTCTTCTCTCTCT+CGG | + | chr3.4:88361494-88361513 | MS.gene41634:CDS | 40.0% | |
| ! | CATGTAATGACGTTTGAGGT+TGG | + | chr3.4:88362274-88362293 | MS.gene41634:CDS | 40.0% |
| ! | CTCCGTCCTAATTTTCTTGA+GGG | + | chr3.4:88361777-88361796 | MS.gene41634:CDS | 40.0% |
| ! | TCTCCGTCCTAATTTTCTTG+AGG | + | chr3.4:88361776-88361795 | MS.gene41634:CDS | 40.0% |
| !! | GATTTTGGGAGTTTGATGAG+AGG | + | chr3.4:88362388-88362407 | MS.gene41634:CDS | 40.0% |
| !!! | CGACAGCATTGAGTTGATTT+TGG | - | chr3.4:88361659-88361678 | None:intergenic | 40.0% |
| ACCATAAGTCCACTTTCTGC+AGG | - | chr3.4:88361602-88361621 | None:intergenic | 45.0% | |
| CGCCCTCAAGAAAATTAGGA+CGG | - | chr3.4:88361782-88361801 | None:intergenic | 45.0% | |
| CGCTCATGTAATGACGTTTG+AGG | + | chr3.4:88362270-88362289 | MS.gene41634:CDS | 45.0% | |
| GGAGAATACCTACAGGAAAC+TGG | - | chr3.4:88361761-88361780 | None:intergenic | 45.0% | |
| GTAGCGCCCTCAAGAAAATT+AGG | - | chr3.4:88361786-88361805 | None:intergenic | 45.0% | |
| GTGGTTCTTCTACTCAGGTT+TGG | + | chr3.4:88361908-88361927 | MS.gene41634:intron | 45.0% | |
| TATATAGGAGCCTGCCAATG+CGG | - | chr3.4:88362316-88362335 | None:intergenic | 45.0% | |
| TCACTCGTAGACCTGTTAAG+TGG | + | chr3.4:88361889-88361908 | MS.gene41634:CDS | 45.0% | |
| TCTCTCGGCTTCATATTCGA+TGG | + | chr3.4:88361509-88361528 | MS.gene41634:CDS | 45.0% | |
| TTAGGACGGAGAATACCTAC+AGG | - | chr3.4:88361768-88361787 | None:intergenic | 45.0% | |
| ! | AAGTGGACTTATGGTACGAC+TGG | + | chr3.4:88361607-88361626 | MS.gene41634:CDS | 45.0% |
| ! | ATGGAGAGTGTAGATGAAGG+AGG | - | chr3.4:88361712-88361731 | None:intergenic | 45.0% |
| ! | TCCTGCAGAAAGTGGACTTA+TGG | + | chr3.4:88361598-88361617 | MS.gene41634:CDS | 45.0% |
| !! | TTATGGTACGACTGGCCTAA+CGG | + | chr3.4:88361615-88361634 | MS.gene41634:CDS | 45.0% |
| AAGGTCGTCCAGTTTCCTGT+AGG | + | chr3.4:88361750-88361769 | MS.gene41634:CDS | 50.0% | |
| GAAACTGGACGACCTTGCAA+TGG | - | chr3.4:88361746-88361765 | None:intergenic | 50.0% | |
| TACGACCTCGAATGGACCAA+CGG | + | chr3.4:88361684-88361703 | MS.gene41634:CDS | 50.0% | |
| TCGTCGTTTACGACCTCGAA+TGG | + | chr3.4:88361676-88361695 | MS.gene41634:CDS | 50.0% | |
| TTCGATGGCTGAGCCAATTC+CGG | + | chr3.4:88361524-88361543 | MS.gene41634:CDS | 50.0% | |
| ! | GGGATGGAGAGTGTAGATGA+AGG | - | chr3.4:88361715-88361734 | None:intergenic | 50.0% |
| !! | CTTGAGGGCGCTACGTATTT+AGG | + | chr3.4:88361792-88361811 | MS.gene41634:CDS | 50.0% |
| !! | GTAGATGAAGGAGGTACCGT+TGG | - | chr3.4:88361703-88361722 | None:intergenic | 50.0% |
| ACCTTGCAATGGCGGTCTGA+GGG | - | chr3.4:88361735-88361754 | None:intergenic | 55.0% | |
| ACTGGACGACCTTGCAATGG+CGG | - | chr3.4:88361743-88361762 | None:intergenic | 55.0% | |
| CACTGCTTGAGGAACCGCAT+TGG | + | chr3.4:88362299-88362318 | MS.gene41634:CDS | 55.0% | |
| CTGAGCCAATTCCGGCTGTT+TGG | + | chr3.4:88361532-88361551 | MS.gene41634:CDS | 55.0% | |
| ! | AGGTACCGTTGGTCCATTCG+AGG | - | chr3.4:88361692-88361711 | None:intergenic | 55.0% |
| !! | TGAGGTTGGAGCACTGCTTG+AGG | + | chr3.4:88362288-88362307 | MS.gene41634:CDS | 55.0% |
| AACTGCTCCGGCCAAACAGC+CGG | - | chr3.4:88361546-88361565 | None:intergenic | 60.0% | |
| GACCTTGCAATGGCGGTCTG+AGG | - | chr3.4:88361736-88361755 | None:intergenic | 60.0% | |
| GAGCGGAGTCCTGCAGAAAG+TGG | + | chr3.4:88361590-88361609 | MS.gene41634:CDS | 60.0% | |
| GCCAATTCCGGCTGTTTGGC+CGG | + | chr3.4:88361536-88361555 | MS.gene41634:CDS | 60.0% | |
| TCCCTCAGACCGCCATTGCA+AGG | + | chr3.4:88361731-88361750 | MS.gene41634:CDS | 60.0% | |
| TCCGGCCAAACAGCCGGAAT+TGG | - | chr3.4:88361540-88361559 | None:intergenic | 60.0% | |
| TGCAATGGCGGTCTGAGGGA+TGG | - | chr3.4:88361731-88361750 | None:intergenic | 60.0% | |
| ! | GCTTGAGGAACCGCATTGGC+AGG | + | chr3.4:88362303-88362322 | MS.gene41634:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 88361453 | 88362435 | 88361453 | ID=MS.gene41634 |
| chr3.4 | mRNA | 88361453 | 88362435 | 88361453 | ID=MS.gene41634.t1;Parent=MS.gene41634 |
| chr3.4 | exon | 88361453 | 88361924 | 88361453 | ID=MS.gene41634.t1.exon1;Parent=MS.gene41634.t1 |
| chr3.4 | CDS | 88361453 | 88361924 | 88361453 | ID=cds.MS.gene41634.t1;Parent=MS.gene41634.t1 |
| chr3.4 | exon | 88362257 | 88362435 | 88362257 | ID=MS.gene41634.t1.exon2;Parent=MS.gene41634.t1 |
| chr3.4 | CDS | 88362257 | 88362435 | 88362257 | ID=cds.MS.gene41634.t1;Parent=MS.gene41634.t1 |
| Gene Sequence |
| Protein sequence |
