Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42685.t1 | RHN70500.1 | 95.8 | 120 | 5 | 0 | 1 | 120 | 24 | 143 | 3.20E-50 | 207.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42685.t1 | G7J8E2 | 95.8 | 120 | 5 | 0 | 1 | 120 | 1 | 120 | 2.3e-50 | 207.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene42685 | 0.809342 | 1.98E-50 | -1.69E-46 |
| MS.gene049171 | MS.gene42685 | 0.836181 | 1.09E-56 | -1.69E-46 |
| MS.gene049173 | MS.gene42685 | 0.850717 | 1.42E-60 | -1.69E-46 |
| MS.gene049217 | MS.gene42685 | 0.866295 | 3.17E-65 | -1.69E-46 |
| MS.gene049222 | MS.gene42685 | 0.849521 | 3.07E-60 | -1.69E-46 |
| MS.gene049285 | MS.gene42685 | 0.867601 | 1.21E-65 | -1.69E-46 |
| MS.gene049320 | MS.gene42685 | 0.893628 | 5.26E-75 | -1.69E-46 |
| MS.gene049369 | MS.gene42685 | 0.809804 | 1.58E-50 | -1.69E-46 |
| MS.gene04939 | MS.gene42685 | 0.803104 | 4.09E-49 | -1.69E-46 |
| MS.gene049427 | MS.gene42685 | 0.874187 | 8.22E-68 | -1.69E-46 |
| MS.gene049491 | MS.gene42685 | 0.818307 | 2.10E-52 | -1.69E-46 |
| MS.gene049609 | MS.gene42685 | 0.869422 | 3.13E-66 | -1.69E-46 |
| MS.gene049614 | MS.gene42685 | 0.83117 | 1.94E-55 | -1.69E-46 |
| MS.gene049633 | MS.gene42685 | 0.817566 | 3.09E-52 | -1.69E-46 |
| MS.gene049661 | MS.gene42685 | 0.833168 | 6.23E-56 | -1.69E-46 |
| MS.gene049662 | MS.gene42685 | 0.819993 | 8.67E-53 | -1.69E-46 |
| MS.gene049760 | MS.gene42685 | 0.821835 | 3.27E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene42685 | 0.867107 | 1.75E-65 | -1.69E-46 |
| MS.gene050076 | MS.gene42685 | 0.85522 | 7.30E-62 | -1.69E-46 |
| MS.gene05010 | MS.gene42685 | 0.800036 | 1.74E-48 | -1.69E-46 |
| MS.gene050163 | MS.gene42685 | 0.883481 | 4.33E-71 | -1.69E-46 |
| MS.gene050165 | MS.gene42685 | 0.865344 | 6.32E-65 | -1.69E-46 |
| MS.gene050333 | MS.gene42685 | 0.852651 | 4.01E-61 | -1.69E-46 |
| MS.gene050353 | MS.gene42685 | -0.805044 | 1.61E-49 | -1.69E-46 |
| MS.gene050397 | MS.gene42685 | 0.803215 | 3.88E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene42685 | 0.89764 | 1.16E-76 | -1.69E-46 |
| MS.gene050504 | MS.gene42685 | 0.841829 | 3.75E-58 | -1.69E-46 |
| MS.gene050518 | MS.gene42685 | 0.859219 | 4.81E-63 | -1.69E-46 |
| MS.gene050538 | MS.gene42685 | 0.8222 | 2.69E-53 | -1.69E-46 |
| MS.gene050593 | MS.gene42685 | 0.852206 | 5.37E-61 | -1.69E-46 |
| MS.gene050612 | MS.gene42685 | 0.868417 | 6.63E-66 | -1.69E-46 |
| MS.gene050662 | MS.gene42685 | 0.809535 | 1.80E-50 | -1.69E-46 |
| MS.gene050675 | MS.gene42685 | 0.848615 | 5.48E-60 | -1.69E-46 |
| MS.gene050686 | MS.gene42685 | 0.824321 | 8.60E-54 | -1.69E-46 |
| MS.gene050699 | MS.gene42685 | 0.861831 | 7.78E-64 | -1.69E-46 |
| MS.gene050747 | MS.gene42685 | 0.827437 | 1.57E-54 | -1.69E-46 |
| MS.gene050767 | MS.gene42685 | 0.897666 | 1.13E-76 | -1.69E-46 |
| MS.gene050770 | MS.gene42685 | 0.897636 | 1.16E-76 | -1.69E-46 |
| MS.gene050772 | MS.gene42685 | 0.891633 | 3.32E-74 | -1.69E-46 |
| MS.gene05080 | MS.gene42685 | 0.826132 | 3.21E-54 | -1.69E-46 |
| MS.gene050950 | MS.gene42685 | 0.833875 | 4.15E-56 | -1.69E-46 |
| MS.gene050986 | MS.gene42685 | 0.850996 | 1.18E-60 | -1.69E-46 |
| MS.gene05100 | MS.gene42685 | 0.855608 | 5.63E-62 | -1.69E-46 |
| MS.gene05101 | MS.gene42685 | 0.836689 | 8.08E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene42685 | 0.854535 | 1.15E-61 | -1.69E-46 |
| MS.gene051228 | MS.gene42685 | 0.890405 | 1.01E-73 | -1.69E-46 |
| MS.gene051387 | MS.gene42685 | 0.810761 | 9.82E-51 | -1.69E-46 |
| MS.gene051434 | MS.gene42685 | 0.816549 | 5.22E-52 | -1.69E-46 |
| MS.gene051551 | MS.gene42685 | 0.83237 | 9.83E-56 | -1.69E-46 |
| MS.gene051617 | MS.gene42685 | 0.820118 | 8.12E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene42685 | 0.857395 | 1.68E-62 | -1.69E-46 |
| MS.gene051692 | MS.gene42685 | 0.842605 | 2.34E-58 | -1.69E-46 |
| MS.gene051694 | MS.gene42685 | 0.809069 | 2.27E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene42685 | 0.919735 | 3.10E-87 | -1.69E-46 |
| MS.gene051858 | MS.gene42685 | 0.854014 | 1.63E-61 | -1.69E-46 |
| MS.gene051949 | MS.gene42685 | 0.807381 | 5.20E-50 | -1.69E-46 |
| MS.gene052078 | MS.gene42685 | 0.806268 | 8.93E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene42685 | 0.901389 | 2.81E-78 | -1.69E-46 |
| MS.gene052290 | MS.gene42685 | 0.844828 | 5.93E-59 | -1.69E-46 |
| MS.gene052310 | MS.gene42685 | 0.81475 | 1.31E-51 | -1.69E-46 |
| MS.gene052322 | MS.gene42685 | 0.803594 | 3.24E-49 | -1.69E-46 |
| MS.gene052379 | MS.gene42685 | 0.872134 | 4.02E-67 | -1.69E-46 |
| MS.gene052421 | MS.gene42685 | 0.811486 | 6.84E-51 | -1.69E-46 |
| MS.gene052481 | MS.gene42685 | 0.837795 | 4.21E-57 | -1.69E-46 |
| MS.gene052484 | MS.gene42685 | 0.873363 | 1.56E-67 | -1.69E-46 |
| MS.gene052485 | MS.gene42685 | 0.80134 | 9.43E-49 | -1.69E-46 |
| MS.gene052510 | MS.gene42685 | 0.800977 | 1.12E-48 | -1.69E-46 |
| MS.gene052615 | MS.gene42685 | 0.875616 | 2.68E-68 | -1.69E-46 |
| MS.gene052670 | MS.gene42685 | 0.800687 | 1.28E-48 | -1.69E-46 |
| MS.gene052916 | MS.gene42685 | 0.844914 | 5.63E-59 | -1.69E-46 |
| MS.gene053009 | MS.gene42685 | 0.883221 | 5.39E-71 | -1.69E-46 |
| MS.gene053103 | MS.gene42685 | 0.861448 | 1.02E-63 | -1.69E-46 |
| MS.gene053104 | MS.gene42685 | 0.898248 | 6.39E-77 | -1.69E-46 |
| MS.gene053107 | MS.gene42685 | 0.814068 | 1.86E-51 | -1.69E-46 |
| MS.gene053207 | MS.gene42685 | 0.886129 | 4.47E-72 | -1.69E-46 |
| MS.gene053209 | MS.gene42685 | 0.858873 | 6.11E-63 | -1.69E-46 |
| MS.gene053213 | MS.gene42685 | 0.898346 | 5.80E-77 | -1.69E-46 |
| MS.gene053286 | MS.gene42685 | 0.891036 | 5.73E-74 | -1.69E-46 |
| MS.gene053322 | MS.gene42685 | 0.901799 | 1.86E-78 | -1.69E-46 |
| MS.gene053449 | MS.gene42685 | 0.816936 | 4.28E-52 | -1.69E-46 |
| MS.gene053561 | MS.gene42685 | -0.809138 | 2.19E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene42685 | 0.859412 | 4.21E-63 | -1.69E-46 |
| MS.gene053632 | MS.gene42685 | 0.864782 | 9.49E-65 | -1.69E-46 |
| MS.gene053813 | MS.gene42685 | 0.818437 | 1.96E-52 | -1.69E-46 |
| MS.gene053915 | MS.gene42685 | 0.806848 | 6.74E-50 | -1.69E-46 |
| MS.gene053916 | MS.gene42685 | 0.825872 | 3.70E-54 | -1.69E-46 |
| MS.gene054376 | MS.gene42685 | 0.894742 | 1.85E-75 | -1.69E-46 |
| MS.gene054408 | MS.gene42685 | 0.806906 | 6.55E-50 | -1.69E-46 |
| MS.gene054600 | MS.gene42685 | 0.869251 | 3.56E-66 | -1.69E-46 |
| MS.gene054642 | MS.gene42685 | 0.908789 | 1.16E-81 | -1.69E-46 |
| MS.gene054802 | MS.gene42685 | 0.835715 | 1.43E-56 | -1.69E-46 |
| MS.gene054803 | MS.gene42685 | 0.834774 | 2.47E-56 | -1.69E-46 |
| MS.gene054804 | MS.gene42685 | 0.838877 | 2.21E-57 | -1.69E-46 |
| MS.gene054927 | MS.gene42685 | 0.810848 | 9.40E-51 | -1.69E-46 |
| MS.gene054954 | MS.gene42685 | 0.834465 | 2.95E-56 | -1.69E-46 |
| MS.gene05505 | MS.gene42685 | 0.841927 | 3.53E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene42685 | 0.820531 | 6.53E-53 | -1.69E-46 |
| MS.gene055154 | MS.gene42685 | -0.809117 | 2.22E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene42685 | 0.861117 | 1.29E-63 | -1.69E-46 |
| MS.gene055212 | MS.gene42685 | 0.838845 | 2.26E-57 | -1.69E-46 |
| MS.gene055254 | MS.gene42685 | 0.861922 | 7.30E-64 | -1.69E-46 |
| MS.gene055277 | MS.gene42685 | 0.82902 | 6.50E-55 | -1.69E-46 |
| MS.gene055289 | MS.gene42685 | 0.838873 | 2.22E-57 | -1.69E-46 |
| MS.gene055362 | MS.gene42685 | 0.874836 | 4.94E-68 | -1.69E-46 |
| MS.gene055397 | MS.gene42685 | 0.894503 | 2.32E-75 | -1.69E-46 |
| MS.gene055412 | MS.gene42685 | 0.825189 | 5.37E-54 | -1.69E-46 |
| MS.gene055499 | MS.gene42685 | 0.911262 | 7.42E-83 | -1.69E-46 |
| MS.gene055502 | MS.gene42685 | 0.858066 | 1.06E-62 | -1.69E-46 |
| MS.gene055577 | MS.gene42685 | 0.835339 | 1.78E-56 | -1.69E-46 |
| MS.gene055636 | MS.gene42685 | 0.849814 | 2.54E-60 | -1.69E-46 |
| MS.gene055638 | MS.gene42685 | 0.840114 | 1.06E-57 | -1.69E-46 |
| MS.gene055647 | MS.gene42685 | 0.888439 | 5.90E-73 | -1.69E-46 |
| MS.gene055722 | MS.gene42685 | 0.898377 | 5.63E-77 | -1.69E-46 |
| MS.gene05592 | MS.gene42685 | 0.905945 | 2.51E-80 | -1.69E-46 |
| MS.gene055941 | MS.gene42685 | 0.826596 | 2.49E-54 | -1.69E-46 |
| MS.gene055956 | MS.gene42685 | -0.820526 | 6.55E-53 | -1.69E-46 |
| MS.gene056083 | MS.gene42685 | 0.85521 | 7.35E-62 | -1.69E-46 |
| MS.gene056191 | MS.gene42685 | 0.818153 | 2.27E-52 | -1.69E-46 |
| MS.gene056374 | MS.gene42685 | 0.902664 | 7.70E-79 | -1.69E-46 |
| MS.gene056410 | MS.gene42685 | 0.817158 | 3.81E-52 | -1.69E-46 |
| MS.gene056422 | MS.gene42685 | 0.809998 | 1.43E-50 | -1.69E-46 |
| MS.gene056448 | MS.gene42685 | 0.800963 | 1.13E-48 | -1.69E-46 |
| MS.gene056487 | MS.gene42685 | 0.807338 | 5.31E-50 | -1.69E-46 |
| MS.gene056510 | MS.gene42685 | 0.825051 | 5.79E-54 | -1.69E-46 |
| MS.gene056511 | MS.gene42685 | 0.811731 | 6.05E-51 | -1.69E-46 |
| MS.gene056549 | MS.gene42685 | 0.80742 | 5.10E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene42685 | 0.85065 | 1.48E-60 | -1.69E-46 |
| MS.gene056657 | MS.gene42685 | 0.884906 | 1.28E-71 | -1.69E-46 |
| MS.gene056897 | MS.gene42685 | 0.86136 | 1.08E-63 | -1.69E-46 |
| MS.gene056946 | MS.gene42685 | 0.80026 | 1.57E-48 | -1.69E-46 |
| MS.gene057011 | MS.gene42685 | 0.852607 | 4.13E-61 | -1.69E-46 |
| MS.gene057076 | MS.gene42685 | 0.846816 | 1.71E-59 | -1.69E-46 |
| MS.gene057124 | MS.gene42685 | 0.816616 | 5.05E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene42685 | 0.916027 | 2.92E-85 | -1.69E-46 |
| MS.gene057146 | MS.gene42685 | 0.84163 | 4.23E-58 | -1.69E-46 |
| MS.gene057196 | MS.gene42685 | 0.813809 | 2.12E-51 | -1.69E-46 |
| MS.gene057246 | MS.gene42685 | 0.830205 | 3.35E-55 | -1.69E-46 |
| MS.gene057295 | MS.gene42685 | 0.821331 | 4.27E-53 | -1.69E-46 |
| MS.gene057331 | MS.gene42685 | 0.8944 | 2.56E-75 | -1.69E-46 |
| MS.gene05735 | MS.gene42685 | 0.823442 | 1.38E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene42685 | 0.889085 | 3.32E-73 | -1.69E-46 |
| MS.gene057926 | MS.gene42685 | 0.820538 | 6.51E-53 | -1.69E-46 |
| MS.gene058006 | MS.gene42685 | 0.82602 | 3.41E-54 | -1.69E-46 |
| MS.gene058019 | MS.gene42685 | 0.854047 | 1.60E-61 | -1.69E-46 |
| MS.gene058130 | MS.gene42685 | 0.843682 | 1.21E-58 | -1.69E-46 |
| MS.gene058293 | MS.gene42685 | 0.825538 | 4.44E-54 | -1.69E-46 |
| MS.gene058323 | MS.gene42685 | 0.840317 | 9.35E-58 | -1.69E-46 |
| MS.gene058419 | MS.gene42685 | 0.892877 | 1.06E-74 | -1.69E-46 |
| MS.gene058544 | MS.gene42685 | 0.842067 | 3.24E-58 | -1.69E-46 |
| MS.gene058660 | MS.gene42685 | 0.847013 | 1.51E-59 | -1.69E-46 |
| MS.gene058723 | MS.gene42685 | 0.880738 | 4.29E-70 | -1.69E-46 |
| MS.gene058773 | MS.gene42685 | 0.85921 | 4.84E-63 | -1.69E-46 |
| MS.gene058838 | MS.gene42685 | 0.80573 | 1.16E-49 | -1.69E-46 |
| MS.gene05883 | MS.gene42685 | 0.825082 | 5.69E-54 | -1.69E-46 |
| MS.gene058840 | MS.gene42685 | -0.818138 | 2.29E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene42685 | 0.866428 | 2.87E-65 | -1.69E-46 |
| MS.gene059023 | MS.gene42685 | 0.806801 | 6.90E-50 | -1.69E-46 |
| MS.gene059029 | MS.gene42685 | 0.892447 | 1.57E-74 | -1.69E-46 |
| MS.gene05913 | MS.gene42685 | 0.842621 | 2.31E-58 | -1.69E-46 |
| MS.gene05934 | MS.gene42685 | 0.823123 | 1.64E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene42685 | 0.800443 | 1.44E-48 | -1.69E-46 |
| MS.gene059455 | MS.gene42685 | 0.801112 | 1.05E-48 | -1.69E-46 |
| MS.gene059536 | MS.gene42685 | 0.890081 | 1.36E-73 | -1.69E-46 |
| MS.gene059676 | MS.gene42685 | 0.802455 | 5.56E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene42685 | 0.872484 | 3.07E-67 | -1.69E-46 |
| MS.gene059688 | MS.gene42685 | 0.852528 | 4.35E-61 | -1.69E-46 |
| MS.gene059692 | MS.gene42685 | 0.854171 | 1.47E-61 | -1.69E-46 |
| MS.gene059695 | MS.gene42685 | 0.852349 | 4.89E-61 | -1.69E-46 |
| MS.gene059711 | MS.gene42685 | 0.832516 | 9.04E-56 | -1.69E-46 |
| MS.gene059718 | MS.gene42685 | 0.875249 | 3.58E-68 | -1.69E-46 |
| MS.gene059858 | MS.gene42685 | 0.831934 | 1.26E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene42685 | 0.831932 | 1.26E-55 | -1.69E-46 |
| MS.gene059902 | MS.gene42685 | 0.856977 | 2.23E-62 | -1.69E-46 |
| MS.gene059960 | MS.gene42685 | 0.891927 | 2.54E-74 | -1.69E-46 |
| MS.gene059974 | MS.gene42685 | 0.806653 | 7.41E-50 | -1.69E-46 |
| MS.gene060193 | MS.gene42685 | 0.812477 | 4.16E-51 | -1.69E-46 |
| MS.gene060341 | MS.gene42685 | 0.810684 | 1.02E-50 | -1.69E-46 |
| MS.gene060356 | MS.gene42685 | 0.82193 | 3.11E-53 | -1.69E-46 |
| MS.gene060403 | MS.gene42685 | 0.804615 | 1.98E-49 | -1.69E-46 |
| MS.gene060482 | MS.gene42685 | 0.834622 | 2.69E-56 | -1.69E-46 |
| MS.gene060703 | MS.gene42685 | 0.807736 | 4.37E-50 | -1.69E-46 |
| MS.gene060727 | MS.gene42685 | 0.812062 | 5.12E-51 | -1.69E-46 |
| MS.gene060728 | MS.gene42685 | 0.824947 | 6.12E-54 | -1.69E-46 |
| MS.gene060762 | MS.gene42685 | 0.828155 | 1.05E-54 | -1.69E-46 |
| MS.gene060804 | MS.gene42685 | 0.889927 | 1.56E-73 | -1.69E-46 |
| MS.gene060818 | MS.gene42685 | 0.88061 | 4.77E-70 | -1.69E-46 |
| MS.gene060835 | MS.gene42685 | 0.84018 | 1.02E-57 | -1.69E-46 |
| MS.gene060837 | MS.gene42685 | 0.83398 | 3.91E-56 | -1.69E-46 |
| MS.gene06092 | MS.gene42685 | 0.845155 | 4.84E-59 | -1.69E-46 |
| MS.gene061041 | MS.gene42685 | 0.856548 | 2.99E-62 | -1.69E-46 |
| MS.gene061051 | MS.gene42685 | -0.817417 | 3.33E-52 | -1.69E-46 |
| MS.gene061134 | MS.gene42685 | 0.839269 | 1.75E-57 | -1.69E-46 |
| MS.gene061150 | MS.gene42685 | 0.853744 | 1.95E-61 | -1.69E-46 |
| MS.gene061171 | MS.gene42685 | 0.801901 | 7.23E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene42685 | 0.898279 | 6.20E-77 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42685.t1 | MTR_3g104950 | 95.833 | 120 | 5 | 0 | 1 | 120 | 1 | 120 | 4.31e-78 | 226 |
| MS.gene42685.t1 | MTR_1g015290 | 65.323 | 124 | 38 | 2 | 1 | 119 | 1 | 124 | 1.88e-47 | 149 |
| MS.gene42685.t1 | MTR_4g125600 | 57.025 | 121 | 45 | 5 | 1 | 119 | 1 | 116 | 1.86e-34 | 115 |
| MS.gene42685.t1 | MTR_2g041260 | 54.918 | 122 | 49 | 3 | 1 | 119 | 1 | 119 | 8.41e-24 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42685.t1 | AT2G06520 | 52.427 | 103 | 46 | 1 | 20 | 119 | 13 | 115 | 2.31e-24 | 90.1 |
Find 22 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATACTTGGAGCTGTTAT+TGG | 0.192002 | 3.3:+86332519 | MS.gene42685:CDS |
| TTTGACTGTGTGATCCTCTT+TGG | 0.259959 | 3.3:-86332321 | None:intergenic |
| CTATACTTGGAGCTGTTATT+GGG | 0.283981 | 3.3:+86332520 | MS.gene42685:CDS |
| ATTAATTAAGCTCTCTTAAC+CGG | 0.332183 | 3.3:-86332558 | None:intergenic |
| TTTGGTTGTGCGATGGGTAA+TGG | 0.371937 | 3.3:-86332303 | None:intergenic |
| AGATGCATTAATTTCAACCT+TGG | 0.419109 | 3.3:-86332346 | None:intergenic |
| TAATGCATCTTTGAAAGAAA+AGG | 0.433518 | 3.3:+86332359 | MS.gene42685:CDS |
| CCTGATGTGGCTCATGCAGC+TGG | 0.437171 | 3.3:+86332429 | MS.gene42685:CDS |
| ATCCTCTTTGGTTGTGCGAT+GGG | 0.483107 | 3.3:-86332309 | None:intergenic |
| TGGGGTTTCCAACTTTGATC+CGG | 0.483190 | 3.3:+86332539 | MS.gene42685:CDS |
| CCAGCTGCATGAGCCACATC+AGG | 0.505992 | 3.3:-86332429 | None:intergenic |
| GATCCTCTTTGGTTGTGCGA+TGG | 0.509446 | 3.3:-86332310 | None:intergenic |
| CTCCATGGTTATACCTGATG+TGG | 0.524546 | 3.3:+86332416 | MS.gene42685:CDS |
| TCTCTTAACCGGATCAAAGT+TGG | 0.532912 | 3.3:-86332547 | None:intergenic |
| TGCATCCTTGACAGCCTCCA+TGG | 0.539158 | 3.3:+86332401 | MS.gene42685:CDS |
| GCTGTGCTAACAGCTATACT+TGG | 0.540967 | 3.3:+86332507 | MS.gene42685:CDS |
| TATACTTGGAGCTGTTATTG+GGG | 0.562747 | 3.3:+86332521 | MS.gene42685:CDS |
| TATAACCATGGAGGCTGTCA+AGG | 0.564152 | 3.3:-86332406 | None:intergenic |
| TACCCATCGCACAACCAAAG+AGG | 0.604798 | 3.3:+86332307 | MS.gene42685:CDS |
| GATCACACAGTCAAAATCCA+AGG | 0.634917 | 3.3:+86332329 | MS.gene42685:CDS |
| AGCCACATCAGGTATAACCA+TGG | 0.670542 | 3.3:-86332418 | None:intergenic |
| CACATCAGGTATAACCATGG+AGG | 0.712255 | 3.3:-86332415 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGCATCTTTGAAAGAAA+AGG | + | chr3.3:86332359-86332378 | MS.gene42685:CDS | 25.0% |
| AAATGCTTAGCAAAAAGTTC+TGG | - | chr3.3:86332476-86332495 | None:intergenic | 30.0% | |
| AGATGCATTAATTTCAACCT+TGG | - | chr3.3:86332349-86332368 | None:intergenic | 30.0% | |
| TAATGGCCTGAAAAATGATT+CGG | - | chr3.3:86332289-86332308 | None:intergenic | 30.0% | |
| ! | TTTTTGCTAAGCATTTTTGC+TGG | + | chr3.3:86332480-86332499 | MS.gene42685:CDS | 30.0% |
| !! | ATGGATTTTTCAGCTTGTTT+TGG | - | chr3.3:86332263-86332282 | None:intergenic | 30.0% |
| !! | TGGATTTTTCAGCTTGTTTT+GGG | - | chr3.3:86332262-86332281 | None:intergenic | 30.0% |
| CTATACTTGGAGCTGTTATT+GGG | + | chr3.3:86332520-86332539 | MS.gene42685:CDS | 35.0% | |
| TATACTTGGAGCTGTTATTG+GGG | + | chr3.3:86332521-86332540 | MS.gene42685:CDS | 35.0% | |
| GATCACACAGTCAAAATCCA+AGG | + | chr3.3:86332329-86332348 | MS.gene42685:CDS | 40.0% | |
| GCTATACTTGGAGCTGTTAT+TGG | + | chr3.3:86332519-86332538 | MS.gene42685:CDS | 40.0% | |
| TCTCTTAACCGGATCAAAGT+TGG | - | chr3.3:86332550-86332569 | None:intergenic | 40.0% | |
| TTTGACTGTGTGATCCTCTT+TGG | - | chr3.3:86332324-86332343 | None:intergenic | 40.0% | |
| ! | CTGAAAAATGATTCGGAGGA+TGG | - | chr3.3:86332282-86332301 | None:intergenic | 40.0% |
| ! | TTGCTAAGCATTTTTGCTGG+TGG | + | chr3.3:86332483-86332502 | MS.gene42685:CDS | 40.0% |
| !! | AGCAAAAAGTTCTGGAGAGA+TGG | - | chr3.3:86332468-86332487 | None:intergenic | 40.0% |
| !! | CATCCTCCGAATCATTTTTC+AGG | + | chr3.3:86332280-86332299 | MS.gene42685:CDS | 40.0% |
| AGCCACATCAGGTATAACCA+TGG | - | chr3.3:86332421-86332440 | None:intergenic | 45.0% | |
| ATCCTCTTTGGTTGTGCGAT+GGG | - | chr3.3:86332312-86332331 | None:intergenic | 45.0% | |
| CACATCAGGTATAACCATGG+AGG | - | chr3.3:86332418-86332437 | None:intergenic | 45.0% | |
| CTCCATGGTTATACCTGATG+TGG | + | chr3.3:86332416-86332435 | MS.gene42685:CDS | 45.0% | |
| GCTGTGCTAACAGCTATACT+TGG | + | chr3.3:86332507-86332526 | MS.gene42685:CDS | 45.0% | |
| TATAACCATGGAGGCTGTCA+AGG | - | chr3.3:86332409-86332428 | None:intergenic | 45.0% | |
| TGGGGTTTCCAACTTTGATC+CGG | + | chr3.3:86332539-86332558 | MS.gene42685:CDS | 45.0% | |
| TTTGGTTGTGCGATGGGTAA+TGG | - | chr3.3:86332306-86332325 | None:intergenic | 45.0% | |
| ! | TGGCCTGAAAAATGATTCGG+AGG | - | chr3.3:86332286-86332305 | None:intergenic | 45.0% |
| !! | TTTTGGGTAGCAGAACTCAC+TGG | - | chr3.3:86332246-86332265 | None:intergenic | 45.0% |
| GATCCTCTTTGGTTGTGCGA+TGG | - | chr3.3:86332313-86332332 | None:intergenic | 50.0% | |
| TACCCATCGCACAACCAAAG+AGG | + | chr3.3:86332307-86332326 | MS.gene42685:CDS | 50.0% | |
| TGCATCCTTGACAGCCTCCA+TGG | + | chr3.3:86332401-86332420 | MS.gene42685:CDS | 55.0% | |
| CCAGCTGCATGAGCCACATC+AGG | - | chr3.3:86332432-86332451 | None:intergenic | 60.0% | |
| CCTGATGTGGCTCATGCAGC+TGG | + | chr3.3:86332429-86332448 | MS.gene42685:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 86332213 | 86332575 | 86332213 | ID=MS.gene42685 |
| chr3.3 | mRNA | 86332213 | 86332575 | 86332213 | ID=MS.gene42685.t1;Parent=MS.gene42685 |
| chr3.3 | exon | 86332213 | 86332575 | 86332213 | ID=MS.gene42685.t1.exon1;Parent=MS.gene42685.t1 |
| chr3.3 | CDS | 86332213 | 86332575 | 86332213 | ID=cds.MS.gene42685.t1;Parent=MS.gene42685.t1 |
| Gene Sequence |
| Protein sequence |
