AlfalfaGEDB | Msa1205560

M. sativa cultivar zhongmu-4 / Msa1205560

KEGG: -
GO: -
PFAM: PF07534.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1205560	G7JGH6	99.127	229	2	0	1	229	1	229	6.24e-168	471

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000180	Msa1205560	0.814101	1.829121e-51	-8.615850e-47
Msa0000220	Msa1205560	0.881947	1.570603e-70	-8.615850e-47
Msa0001040	Msa1205560	0.811291	7.535035e-51	-8.615850e-47
Msa0001510	Msa1205560	0.858551	7.622746e-63	-8.615850e-47
Msa0001540	Msa1205560	0.833719	4.538436e-56	-8.615850e-47
Msa0002690	Msa1205560	0.840162	1.026447e-57	-8.615850e-47
Msa0002700	Msa1205560	0.831833	1.333979e-55	-8.615850e-47
Msa0003340	Msa1205560	0.804656	1.945001e-49	-8.615850e-47
Msa0004180	Msa1205560	0.822177	2.723641e-53	-8.615850e-47
Msa0004340	Msa1205560	0.826113	3.242358e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1205560	MtrunA17_Chr4g0054781	99.127	229	2	0	1	229	1	229	1.20e-171	471

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1205560	AT1G32520.1	73.640	239	53	7	1	229	1	239	6.73e-121	343

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Find 69 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGCCATGTGTAATAAT+AGG	0.265542	8_1:-61899554	Msa1205560:CDS
AATGGTCTATAATTCGGATT+TGG	0.308738	8_1:-61898788	Msa1205560:three_prime_UTR
ACAAATCACATACTATTTCA+TGG	0.441288	8_1:+61898862	None:intergenic
ACAAGTCCATATTGTTTATT+TGG	0.118956	8_1:-61900137	Msa1205560:CDS
ACAGACAACTCTTTCAAGTT+TGG	0.342835	8_1:-61899527	Msa1205560:CDS
ACCCATAACTGGGGCTAATG+GGG	0.505857	8_1:+61899316	None:intergenic
AGACAAGCCAAGTCTAGATT+GGG	0.460900	8_1:-61899251	Msa1205560:CDS
AGACAATCCCAATCTAGACT+TGG	0.587589	8_1:+61899244	None:intergenic
AGCAAAACCACCCATAACTG+GGG	0.696608	8_1:+61899307	None:intergenic
AGCCCATCAGCCCCGAATTG+CGG	0.532569	8_1:+61899350	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATACGGATAAGATGATGAAT+TGG	-	chr8_1:61898741-61898760	Msa1205560:three_prime_UTR	30.0%
CAAATTCTCTATCATTCCAA+TGG	-	chr8_1:61898843-61898862	Msa1205560:three_prime_UTR	30.0%
GTCCATATTGTTTATTTGGT+TGG	-	chr8_1:61898876-61898895	Msa1205560:three_prime_UTR	30.0%
TGTTTATTTGGTTGGAACAT+AGG	-	chr8_1:61898884-61898903	Msa1205560:three_prime_UTR	30.0%
ACAAATACATGTAAAGCCTA+AGG	+	chr8_1:61899334-61899353	None:intergenic	30.0%
GTGTAACCTATTATTACACA+TGG	+	chr8_1:61899464-61899483	None:intergenic	30.0%
CTTGATCCAAAAGTCTAAAT+CGG	+	chr8_1:61899976-61899995	None:intergenic	30.0%
ATAAGTAGTTGTATCCTTCA+AGG	-	chr8_1:61900181-61900200	Msa1205560:CDS	30.0%
GTATGTAAATGTGTGTCTAA+TGG	-	chr8_1:61900203-61900222	Msa1205560:exon	30.0%
GTGTCTAATGGTCTATAATT+CGG	-	chr8_1:61900215-61900234	Msa1205560:five_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	61898719	61900312	61898719	ID=Msa1205560;Name=Msa1205560
chr8_1	mRNA	61898719	61900312	61898719	ID=Msa1205560-mRNA-1;Parent=Msa1205560;Name=Msa1205560-mRNA-1;_AED=0.01;_eAED=0.01;_QI=108\|1\|1\|1\|1\|1\|2\|417\|229
chr8_1	exon	61898719	61899653	61898719	ID=Msa1205560-mRNA-1:exon:20090;Parent=Msa1205560-mRNA-1
chr8_1	exon	61900033	61900312	61900033	ID=Msa1205560-mRNA-1:exon:20089;Parent=Msa1205560-mRNA-1
chr8_1	five_prime_UTR	61900205	61900312	61900205	ID=Msa1205560-mRNA-1:five_prime_utr;Parent=Msa1205560-mRNA-1
chr8_1	CDS	61900033	61900204	61900033	ID=Msa1205560-mRNA-1:cds;Parent=Msa1205560-mRNA-1
chr8_1	CDS	61899136	61899653	61899136	ID=Msa1205560-mRNA-1:cds;Parent=Msa1205560-mRNA-1
chr8_1	three_prime_UTR	61898719	61899135	61898719	ID=Msa1205560-mRNA-1:three_prime_utr;Parent=Msa1205560-mRNA-1

Gene Sequence

>Msa1205560

ATGGCATCTTGTATCTCAAATTCTCTATCATTCCAATGGAGGAAAACAAGTCCATATTGTTTATTTGGTTGGAACATAGGCAGAAAGAGAGTTGATGACAAACCTCAGATTAAATATCATGATATTGATCTCACTTTCTCAACTTCTCTAGTTAACAAAACATTTTTGAAAGGTAAGGAACTGAAATGCTGCTACAGGGCAACAATTGATGGATTTAGTGCAACAAATTTCCACCAATGTTGTGATTTTAAAGGGCCATGTGTAATAATAGGTTACACAGACAACTCTTTCAAGTTTGGTGCATTTAACCCTGAAGGATATAGAAGCACAGATGATTACTATGAAACATTTGATGCATTTCTATTTTATTGGGTAGAAAATAAAAATGAACCAATAATTTTGCCTAAGGTTGGTGGCAGTGGTGCAGCTCTGTTTGATTATGCTCGTGGTGGGCCGCAATTCGGGGCTGATGGGCTTCTCATCGGGCCCCCATTAGCCCCAGTTATGGGTGGTTTTGCTGGGCCTGATACTAATTCTGGTGTTGGTGACTTGAGACAAGCCAAGTCTAGATTGGGATTGTCTTATGCCAAAAGGGAAGATGGCAAGGAGTCTATTTTTGGTGATGAGTCTAGGGCAACTATTCAAGAAGTAGAAGTGTTTTGTAGCCCCAAAATTGCAAGTTTATACTAG

Protein sequence

>Msa1205560

MASCISNSLSFQWRKTSPYCLFGWNIGRKRVDDKPQIKYHDIDLTFSTSLVNKTFLKGKELKCCYRATIDGFSATNFHQCCDFKGPCVIIGYTDNSFKFGAFNPEGYRSTDDYYETFDAFLFYWVENKNEPIILPKVGGSGAALFDYARGGPQFGADGLLIGPPLAPVMGGFAGPDTNSGVGDLRQAKSRLGLSYAKREDGKESIFGDESRATIQEVEVFCSPKIASLY*